Papers -
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Combined Effects of Methylated Cytosine and Molecular Crowding on the Thermodynamic Stability of DNA Duplexes Invited Reviewed
Combined Effects of Methylated Cytosine, Molecular Crowding, on, the Thermodynamic Stability of, DNA Duplexes
Int. J. Mol. Sci. 22 ( 2 ) 947 2021.1
Joint Work
DOI： 10.3390/ijms22020947
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Exploration of structural transition phenomenon in flexible metal-organic framework formed on polymer substrate
S. Hirao, R. Hamagami, T. Ohhashi, K. Eguchi, N. Kubo, Y. Takashima, K. Akamatsu, T. Tsuruoka
CrystEngComm 23 8498 - 8505 2021
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Precisely Controlled Reproducible Synthesis of Palladium Nanoparticles inside Metal-Organic Frameworks with H2 Gas as Reductant: Effects of Framework Crystallinity and H2 Gas
Y. Takashima, Y. Sato, N.Kubo, T. Tsuruoka, K. Akamatsu
Chem. Lett 50 244 - 247 2021
Joint Work
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Controlling Interfacial Ion-Transport Kinetics Using Polyelectrolyte Membranes for Additive- and Effluent-free, High-Performance Electrodeposition Reviewed
Kensuke Akamatsu, Shu-ichi Nakano, Koshi Kimura, Yohei Takashima, Takaaki Tsuruoka, Hidemi Nawafune, Yuki Sato, Junya Murai, Hiroshi Yanagimoto
ACS Applied Materials&Interfaces 2021
Joint Work
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Ozaki, M., Yoshida, S., Tsuruoka, T., Usui, K.
Chemical Communications 57 2021
Joint Work
Authorship：Last author, Corresponding author
We developed a system to directly produce gold nanoparticles in cells by intracellular mineralization in lower concentration than conventional methods using a peptide consisting of a cell-penetrating sequence and a gold ion-binding sequence. Furthermore, we could control the uniquely shaped gold nanostructures that were produced by changing peptide structures.
DOI： 10.1039/d0cc06117d
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Elemental composition control of gold-titania nanocomposites by site-specific mineralization using artificial peptides and DNA Reviewed International journal
Ozaki, M., Imai, T., Tsuruoka, T., Sakashita, S., Tomizaki, K.-Y., Usui, K.
Communications Chemistry 4 2021
Joint Work
Authorship：Last author, Corresponding author
Biomineralization, the precipitation of various inorganic compounds in biological systems, can be regulated in terms of the size, morphology, and crystal structure of these compounds by biomolecules such as proteins and peptides. However, it is difficult to construct complex inorganic nanostructures because they precipitate randomly in solution. Here, we report that the elemental composition of inorganic nanocomposites can be controlled by site-specific mineralization by changing the number of two inorganic-precipitating peptides bound to DNA. With a focus on gold and titania, we constructed a gold-titania photocatalyst that responds to visible light excitation. Both microscale and macroscale observations revealed that the elemental composition of this gold-titania nanocomposite can be controlled in several ten nm by changing the DNA length and the number of peptide binding sites on the DNA. Furthermore, photocatalytic activity and cell death induction effect under visible light (>450 nm) irradiation of the manufactured gold-titania nanocomposite was higher than that of commercial gold-titania and titania. Thus, we have succeeded in forming titania precipitates on a DNA terminus and gold precipitates site-specifically on double-stranded DNA as intended. Such nanometer-scale control of biomineralization represent a powerful and efficient tool for use in nanotechnology, electronics, ecology, medical science, and biotechnology.
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Shoya Hirao, Ruho Hamagami, Takashi Ohhashi, Keiichi Eguchi, Neo Kubo, Yohei Takashima, Kensuke Akamatsu, Takaaki Tsuruoka
CrystEngComm 23 ( 48 ) 8498 - 8505 2021
Authorship：Last author, Corresponding author Publisher：Royal Society of Chemistry (RSC)
We investigate the structural transition of directly formed flexible MOF crystals on a polymer substrate.
DOI： 10.1039/d1ce01383a
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Precisely Controlled Reproducible Synthesis of Palladium Nanoparticles inside Metal-Organic Frameworks with H2 Gas as Reductant: Effects of Framework Crystallinity and H2 Gas Reviewed
Y. Takashima, Y. Sato, N.Kubo, T. Tsuruoka, K. Akamatsu
Chem. Lett 50 244 - 247 2021
Joint Work
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Intracellular mineralization of gold nanoparticles using gold ion-binding peptides with cell-penetrating ability Reviewed
Makoto Ozaki, Shuhei Yoshida, Takaaki Tsuruoka, Kenji Usui
Chemical Communications 2021
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Photosensitizers Based on G-Quadruplex Ligand for Cancer Photodynamic Therapy Invited International journal
Keiko Kawauchi, Ryoto Urano, Natsuki Kinoshita, Shin Kuwamoto, Takeru Torii, Yoshiki Hashimoto, Shinya Taniguchi, Mitsuki Tsuruta, Daisuke Miyoshi
Genes 11 ( 11 ) 1340 2020.11
Joint Work
G-quadruplex (G4) is the non-canonical secondary structure of DNA and RNA formed by guanine-rich sequences. G4-forming sequences are abundantly located in telomeric regions and in the promoter and untranslated regions (UTR) of cancer-related genes, such as RAS and MYC. Extensive research has suggested that G4 is a potential molecular target for cancer therapy. Here, we reviewed G4 ligands as photosensitizers for cancer photodynamic therapy (PDT), which is a minimally invasive therapeutic approach. The photosensitizers, such as porphyrins, were found to be highly toxic against cancer cells via the generation of reactive oxidative species (ROS) upon photo-irradiation. Several porphyrin derivatives and analogs, such as phthalocyanines, which can generate ROS upon photo-irradiation, have been reported to act as G4 ligands. Therefore, they have been implicated as promising photosensitizers that can selectively break down cancer-related DNA and RNA forming G4. In this review, we majorly focused on the potential application of G4 ligands as photosensitizers, which would provide a novel strategy for PDT, especially molecularly targeted PDT (mtPDT).
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Precisely Controlled Reproducible Synthesis of Palladium Nanoparticles inside Metal-Organic Frameworks with H2 Gas as Reductant: Effects of Framework Crystallinity and H2 Gas Pressure Reviewed
Yohei Takashima, Yasushi Sato, Neo Kubo, Takaaki Tsuruoka, Kensuke Akamatsu
Chem. Lett. 50 ( 2 ) 244 - 247 2020.11
Authorship：Lead author, Corresponding author
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TakahiroYamauchi, Takafumi Kubodera, Daisuke Miyoshi, Naoki Sugimoto, Shuji Hirohata
Journal of Bioscience and Bioengineering 2020.10
Joint Work
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Mechanism toward Turn-on of Polysaccharide-Porphyrin Complexes for Fluorescence Probes and Photosensitizers in Photodynamic Therapy in Living Cells Reviewed International journal
Shodai Hino, Rikushi Funada, Kouta Sugikawa, Riku Kawasaki, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Atsushi Ikeda
CHEMMEDCHEM 15 ( 1 ) 1 - 12 2020.10
Joint Work
Publisher：WILEY-V C H VERLAG GMBH
beta-(1,3-1,6)-D-Glucan, lambda-carrageenan, tamarind gum, and pullulan can dissolve various porphyrin derivatives via the formation of complexes in water using a high-speed vibration milling method. The aqueous solutions of the resulting complexes exhibit long-term stability. Despite the adverse effects of the self-quenching process, notable fluorescence and improved photodynamic activity of the polysaccharide-complexed porphyrin derivatives were observed in the presence of liposomes, micelles, cyclodextrins, and HeLa cells. It was noted that the type of porphyrins was more important than the type of polysaccharides present in the complex. Porphyrin self-aggregates were monodispersed in the lipid membranes of the liposomes and lysosomes. The polysaccharide-complexed porphyrin derivatives showed increased photodynamic activity toward HeLa cells under photoirradiation between 610 and 740 nm.
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Novel Strategy of Photodynamic Therapy Targeting RAS mRNA with G-Quadruplex Ligands for Cancer Treatment Invited Reviewed International journal
Takeru Torii, Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi
Journal of Data Mining in Genomics & Proteomics 11 ( 2 ) 2020.8
Joint Work
Authorship：Corresponding author
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Dermal fibroblasts internalize phosphatidylserine-exposed secretory melanosome clusters and apoptotic melanocytes. Reviewed
21 ( 16 ) 5789 2020.8
Joint Work
DOI： 10.3390/ijms21165789
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Modification of the Release Rate of Curcumin from the Cavity of Renatured beta-1,3-1,6-Glucan Nanoparticles by Changing the Molecular Weight of the Constituent Polysaccharide Chains Reviewed
Yuki Nonoguchi, Ayumu Kodama, Rika Unesaki, Nanako Doi, Toshio Suzuki, Kazuya Koumoto
CHEMISTRY LETTERS 49 ( 10 ) 1150 - 1153 2020.7
Joint Work
Publisher：CHEMICAL SOC JAPAN
We prepared renatured beta-1,3-1,6-glucan nanoparticles from molecular-weight-fractionated beta-1,3-1,6-glucans to analyze the molecular effects on the inclusion and release of the guest molecule curcumin. Glucan molecular weight strongly affects the amount of curcumin included in the complexes and its release rate. We found that the slow release rate of the incorporated curcumin is attributed not only to the stabilized stacking structure but also the slow migration between the internal and surface cavities characteristic of polymeric materials.
DOI： 10.1246/cl.200366
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Photodynamic therapy targeting RAS mRNA
Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi
Medical Science Digest 46 ( 9 ) 26 - 27 2020.7
Joint Work
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Competing Roles of Two Kinds of Ligand during Nonclassical Crystallization of Pillared-Layer Metal-Organic Frameworks Elucidated Using Microfluidic Systems
Yoko Tanaka, Yu Kitamura, Ryuji Kawano, Kan Shoji, Moe Hiratani, Tetsuo Honma, Hikaru Takaya, Hirofumi Yoshikawa, Takaaki Tsuruoka, Daisuke Tanaka
Chemistry - A European Journal 26 ( 41 ) 8889 - 8896 2020.7
© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim To diversify metal-organic frameworks (MOFs), multi-component MOFs constructed from more than two kinds of bridging ligand have been actively investigated due to the high degree of design freedom afforded by the combination of multiple ligands. Predicting the synthesis conditions for such MOFs requires an understanding of the crystallization mechanism, which has so far remained elusive. In this context, microflow systems are efficient tools for capturing non-equilibrium states as they facilitate precise and efficient mixing with reaction times that correspond to the distance from the mixing point, thus enabling reliable control of non-equilibrium crystallization processes. Herein, we prepared coordination polymers with pillared-layer structures and observed the intermediates in the syntheses with an in-situ measurement system that combines microflow reaction with UV/Vis and X-ray absorption fine-structure spectroscopies, thereby enabling their rapid nucleation to be monitored. Based on the results, a three-step nonclassical nucleation mechanism involving two kinds of intermediate is proposed.
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Different reactivity of Sp and Rp isomers of phosphorothioate-modified oligonucleotides in a duplex structure Reviewed
Md Ariful Islam, Aki Fujisaka, Junji Kawakami, Takao Yamaguchi, Satoshi Obika
Bioorganic Medicinal Chemistry Letters 30 ( 14 ) 127166 - 127166 2020.7
Publisher：Elsevier BV
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Novel Strategy of Photodynamic Therapy Targeting RAS mRNA with G-Quadruplex Ligands for Cancer Treatment Invited Reviewed
Takeru Torii, Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi
Journal of Data Mining in Genomics & Proteomics 2020.6
Joint Work