Papers -
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Cryoprotective ability of betaine-type metabolite analogs during freezing denaturation of enzymes Reviewed
Yuichi Nakagawa, Masahiro Sota, Kazuya Koumoto
Biotechnology Letters 37 1607 - 1613 2015
Joint Work
Authorship：Lead author
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Immunestimulating Activity of Lactobacilli-mimicking Microparticles modified withPolysaccharide-Peptidoglycan Complex derived from L. casei Shirota Strain Invited
Kazuya Koumoto, Koji Nagahama, Jun Matsui
BIOINDUSTRY 32 ( 9 ) 42 - 47 2015
Joint Work
Authorship：Lead author
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Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size Reviewed
Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
Bioconjugate Chemistry 26 ( 8 ) 1775 - 1781 2015
Joint Work
Authorship：Lead author
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Significance of anionic functional group in betaine-type metabolite analogs on the facilitation of enzyme reactions International journal
Yuichi Nakagawa, Kotomi Takagi, Ryutaro Genjima, Kazuya Koumoto
BIOPROCESS AND BIOSYSTEMS ENGINEERING 38 ( 9 ) 1811 - 1817 2015
Joint Work
Authorship：Lead author Publisher：SPRINGER
Using synthetic sulfobetaine library, the enzyme activation behavior has been investigated. Comparison of enzyme activation behavior revealed that sulfobetaines equally facilitate enzyme reactions, being consistent with that of carboxybetaines. The subsequent kinetic and solution property analyses clarified that both the kinetic parameter and hydration property changes are identical with those of carboxybetaines, indicating that the difference in the anionic functional group of the betaine structure scarcely affects the enzyme activation. On the other hand, comparison of carboxy- or sulfo-betaines with tetraalkylammonium salts, whose counteranion binds to the ammonium cation intermolecularly, revealed that the activation ability for enzymes of tetraalkylammonium salts is considerably smaller than that of carboxy- or sulfo-betaines. These findings give us a hint to design the useful betaine-type enzyme activators.
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Enhanced Chromogenic Sensitivity of Horseradish Peroxidase-Catalyzed Oxidative Reactions in the Presence of Betaine-Type Metabolite Analogs Reviewed
Kotomi Takagi, Yasuhiro Kashima, Satoshi Fujii, Kazuya Koumoto
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 88 ( 8 ) 1074 - 1082 2015
Joint Work
Authorship：Lead author Publisher：CHEMICAL SOC JAPAN
Horseradish peroxidase (HRP), a well-known oxidase, is frequently used in the diagnostic field as a labeling enzyme, where it amplifies the substrate binding signal of antibodies. Though practical for detecting moderate amounts of substrate, there is strong demand for further development of its sensitivity to detect minuscule quantities of biomarkers. Recently, we found that betaine-type cellular metabolite analogs facilitate enzymatic hydrolysis just by dissolving them into the reaction buffer. In the present study, using the analog (2-(N,N,N-tri-n-butylammonium) acetate) and various colorimetric substrates of HRP, we investigated the activation behavior of HRP. As a result, the analog structure- and concentration-dependently facilitated the various HRP-catalyzed oxidative reactions. Interestingly, the analog facilitated not only the reaction rate but also the chromogenic sensitivity. Kinetic and structural analyses revealed that the increased chromogenic sensitivity is related to the enhancement of the conformational flexibility in the substrate binding site in HRP by addition of the analog, which diminishes the binding affinity between HRP and large substrates. The finding serves to create practical applications of the analogs for increased detection sensitivity of HRP-related clinical agents.
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DNA structures under molecular crowding conditions with a phosphorylcholine derivative (MPC) Reviewed
Yu-mi Ueda, Tomohiro Konno, Kazuhiko Ishihara, Naoki Sugimoto, Daisuke Miyoshi
Transact. Mat. Res. Soc. Japan 40 99 - 102 2015
Joint Work
Authorship：Lead author
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Effects of background anionic compounds on the activity of the hammerhead ribozyme in Mg2+-unsaturated solutions Reviewed
Shu-ichi Nakano, Yuichi Kitagawa, Daisuke Miyoshi, Naoki Sugimoto
J. Biol. Inorg. Chem. 20 1049 - 1058 2015
Joint Work
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Stabilization of DNA Structures With Poly(ethylene sodium phosphate) Reviewed
R. Moriyama, Y. Iwasaki, D. Miyoshi
J. Phys. Chem. B 119 11969 - 11977 2015
Joint Work
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Effects of cosolvents on the folding and catalytic activities of the hammerhead ribozyme Reviewed
S. Nakano, Y. Kitagawa, H. Yamashita, D. Miyoshi, N. Sugimoto
ChemBioChem 16 1803 - 1810 2015
Joint Work
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A mRNA-responsive G-quadruplex-based drug release system Invited Reviewed
Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, and Naoki Sugimoto
Sensors 15 ( 4 ) 9388 - 9403 2015
Joint Work
Authorship：Lead author
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DNA G-quadruplex detection system employing a protein fibril ligand Invited Reviewed
Ryuichi Maeda, Hidenobu Yaku, Takakazu Nakabayashi, Takashi Murashima, Naoki Sugimoto, Daisuke Miyoshi
Telomere and Telomerase 2 e691 2015
Joint Work
Authorship：Lead author
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DNA structures under molecular crowding conditions with a phosphorylcholine derivative (MPC)
Yu-mi Ueda, Tomohiro Konno, Kazuhiko Ishihara, Naoki Sugimoto, Daisuke Miyoshi
Transact. Mat. Res. Soc. Japan 40 99 - 102 2015
Joint Work
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Stabilization of DNA Structures With Poly(ethylene sodium phosphate)
R. Moriyama, Y. Iwasaki, D. Miyoshi
J. Phys. Chem. B 119 11969 - 11977 2015
Joint Work
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Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface Reviewed
T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu
J. Colloid Interface Sci 451 212 - 215 2015
Joint Work
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Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface Reviewed
T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu
J. Colloid Interface Sci 451 212 - 215 2015
Joint Work
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Metal Nanocrystal/Metal Organic Framework Core/Shgell Nanostructure From Selective Self-Assembly Induced By Localization of Metal Ion Precursors on Nanocrystal Surface
T. Ohhashi, T. Tsuruoka, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu
J. Colloid Interface Sci 451 212 - 215 2015
Joint Work
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Comparison of Kinetic and Thermodynamic Analyses for Duplex Formation
E. Tomita, M. Sumimoto, S. Itoh, M. Takashima, and J. Kawakami
142 - 143 2015
Joint Work
Authorship：Last author, Corresponding author
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Curcumisome nanovesicles generated by self-assembly of curcumin amphiphiles toward cancer theranostics Reviewed
Koji Nagahama, Takayuki Kumano, Naho Oyama, Junji Kawakami
BIOMATERIALS SCIENCE 3 ( 12 ) 1566 - 1578 2015
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Control of guanine-rich DNA secondary structures depending on the protease activity using a designed PNA peptide Reviewed
Kenji Usui, Arisa Okada, Keita Kobayashi, Naoki Sugimoto
ORGANIC & BIOMOLECULAR CHEMISTRY 13 ( 7 ) 2022 - 2025 2015
Publisher：ROYAL SOC CHEMISTRY
We constructed a regulation system for DNA secondary structure formation of G-rich sequences using a designed PNA peptide exhibiting an on-to-off switching functionality, depending on the protease activity. This study introduces the new concept of a simple and powerful system for regulating quadruplex-related important biological events.
DOI： 10.1039/c4ob02535k
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Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface Reviewed
T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu
J. Colloid Interface Sci. 451 212 - 215 2015
Joint Work