Papers -
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Miho Takagi-Sato, Koji Morita, Yoshiyuki Onishi, Yuuka Watahiki, Taku Ishigaki, Tomoka Akita, Erisa Tomita, Junji Kawakami, Makoto Koizumi
Nucleosides, Nucleotides & Nucleic Acids 39 ( 6 ) 838 - 852 2020.6
Publisher：Informa UK Limited
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Comparison of macrophage activation using γ-globulin and serum-derived macrophage activating factor. Reviewed
Akamatsu M, Mashiba R, Kawakatsu K, Ishikawa M, Nishikata T.
Anticancer Research 40 ( 8 ) 4707 - 4710 2020.6
Joint Work
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RNA Phase Separation-Mediated Direction of Molecular Trafficking Under Conditions of Molecular Crowding Invited Reviewed
Kazuki Kohata, Daisuke Miyoshi
Biophys. Rev. 12 669 - 676 2020.5
Joint Work
Living cells are highly crowded with large and small biomolecules. The total concentration of biomolecules can reach 400 mg/ml, and 40% of the cell volume is occupied by biomolecules. Droplet formation in cells via liquid-liquid phase separation may play a role in controlling biochemical reactions in this complex molecular environment. Liquid-liquid phase separation generally involves nucleic acids and proteins as anionic and cationic components, respectively. Significant characteristics of droplets, which make them different from protein aggregation or fibril formation, are reversibility of formation and responsiveness to the molecular environment. In this review, we quantitatively describe the molecular environment inside cells and droplets that participate in controlling central dogma reactions. Finally, we discuss the importance of droplets under conditions of molecular crowding within living cells.
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Development of a chromophore-solid phase peptide reaction assay (C-SPRA) for assessing skin sensitization: In vitro Reviewed International journal
Miyazaki, H., Hamada, Y., Takaishi, H., Minamino, Y., Ikeda, H., Mekata, H., Takaishi, M., Yamashita, K., Usui, K.
Analyst 145 3211 - 3216 2020.5
Joint Work
Authorship：Last author, Corresponding author
We developed an in vitro chromophore-solid phase peptide reaction assay (C-SPRA) using microbead-immobilized peptides and chromophores. Peptide-resins (microbeads) reacted with 14 representative chemicals to demonstrate the test's capacity to predict skin sensitization. C-SPRA enables accurate and high-throughput assessments of various chemicals, including poorly water-soluble sensitizers that are regarded as weakly potent by other methods.
DOI： 10.1039/c9an02514f
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Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA Reviewed
H. Tateishi-Karimata, D. Banerjee, T. Ohyama, S. Matsumoto, D. Miyoshi, S. Nakano, and N. Sugimoto
Biochemical and Biophysical Research Communications 525 ( 1 ) 177 - 183 2020.4
Joint Work
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Coordination-Driven Self-Assembly on Polymer Surfaces for Efficient Synthesis of [Au(SPh)]<inf>n</inf> Coordination Polymer-Based Films
Takaaki Tsuruoka, Takashi Ohhashi, Jumpei Watanabe, Rikuto Yamada, Shoya Hirao, Yohei Takashima, Aude Demessence, Shefali Vaidya, Oleksandra Veselska, Alexandra Fateeva, Kensuke Akamatsu
Crystal Growth and Design 20 ( 3 ) 1961 - 1968 2020.3
Joint Work
Publisher：American Chemical Society ({ACS})
We describe a simple bottom-up strategy to generate [Au(SPh)] (SPh: thiophenolate) coordination polymer (CP) based thin films by employing an interfacial self-assembly on a nylon substrate. This method utilizes Au ion-doped nylon film as a precursor of [Au(SPh)] CP and a support substrate to control the kinetics of the reduction of the Au ion from Au(III) to Au(I) and the complexation of Au(I) ions as well as SPh on the surface of the nylon film. The obtained CPs exhibit a fibril morphology with high crystallinity, which leads to a luminescent property. Further, we systematically study the formation mechanism of [Au(SPh)] CPs on the substrates. Amorphous CPs formed at an early stage of reaction are transformed to crystalline CPs based on the rearrangement of components during the reaction. The findings described herein offer novel and interesting openings for the synthesis of Au ion-based CPs that demonstrate desired functions for a wide range of applications. n n n
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Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA. Reviewed International journal
Hisae Tateishi-Karimata, Dipanwita Banerjee, Tatsuya Ohyama, Saki Matsumoto, Daisuke Miyoshi, Shu-Ich Nakano, Naoki Sugimoto
Biochemical and biophysical research communications 2020.2
Telomeric G-quadruplex topology has the ability to regulate telomerase activity, which counteracts the shortening of telomere with successive cell divisions, thereby causing genomic longevity. However, the detailed mechanism of G-quadruplexes topologies formed by telomeric sequences requires further investigation. In this study, we quantitatively investigated the effect of cosolutes, particularly the varying number of hydroxyl groups, on the structural transition between hybrid type and parallel G-quadruplexes formed by telomeric DNA sequences. Cosolutes with one or no hydroxyl groups in the vicinal position more efficiently induced the transition to parallel G-quadruplex from hybrid G-quadruplex than those with more hydroxyl groups. We also examined the effect of cosolute structures on the hydration of G-quadruplex formation; the results indicated that cosolutes with fewer hydroxyl groups lead to the release of greater amount of water during G-quadruplex formation. Molecular dynamics results showed that the parallel G-quadruplex was more dehydrated than the hybrid type G-quadruplex. Generally, a dehydrated structure is favored under crowding condition. Thus, depending on the surrounding cosolutes, the G-quadruplex topology can be controlled by the G-quadruplex hydration state.
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Solubilization of poorly water-soluble bioactive molecules in neutral aqueous media by complexation with renatured beta-1,3-1,6-glucan nanoparticles Reviewed International journal
Ayumu Kodama, Akifumi Nakagawa, Yuki Nonoguchi, Haruka Sakurai, Chieko Yano, Toshio Suzuki, Kazuya Koumoto
BIOPOLYMERS 111 ( 4 ) e23349 2020.2
Joint Work
Publisher：WILEY
The design of scaffolds for solubilizing/dispersing poorly water-soluble bioactive molecules in neutral aqueous media is a major challenge of functional food, pharmaceuticals, and cosmetics development, as highlighted by the plethora of corresponding solubilization/dispersion strategies. Herein, renatured beta-1,3-1,6-glucan (r-glucan) nanoparticles prepared by neutralization of alkali-denatured beta-1,3-1,6-glucan and subsequent centrifugation are used as a host to disperse water-insoluble bioactive molecules (curcumin, all-trans-retinoic acid, and rebamipide) by simple mixing of host and guest solutions. Curcumin in the r-glucan cavity is found to be stacked in the form of J-aggregates and twisted along the helix, and is demonstrated to be retained for significantly longer than curcumin in the corresponding gamma-cyclodextrin (gamma-CD) complex. Specifically, curcumin incorporated in gamma-CD is released within 5.5 hours, whereas that in the r-glucan complex is released very slowly, with 12% of curcumin in the latter complex retained after 31-day incubation at 37 degrees C. Thus, inclusion protocol simplicity and slow release ability make r-glucan nanoparticles a potential carrier scaffold for various applications.
DOI： 10.1002/bip.23349
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MMP24 as a Target of YAP is a Potential Prognostic Factor in Cancer Patients Reviewed International journal
Wataru Sugimoto, Katsuhiko Ito, Hiroaki Hirata, Yoshinori Abe, Takeru Torii, Yasumasa Mitsui, Yemima Budirahardja, Nobuyuki Tanaka, Keiko Kawauchi
Bioengineering 7 ( 18 ) 1 - 12 2020.2
Joint Work
Authorship：Last author, Corresponding author
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Therapeutic effect of intravenously administered autologous adipose-derived stem cells on chronic stage stroke patients. Reviewed
7 070 2020
Joint Work
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Coordination-Driven Self-Assembly on Polymer Surface for Efficient Synthesis of [Au(SPh)]n Coordination Polymer-Based Films
T. Tsuruoka, T. Ohhashi, J. Watanabe, R. Yamda, S. Hirao, T. Takashima, A. Demessence, S. Vaidya, O. Veselska, A. Fateeva, K. Akamatsu
Cryst. Growth 1961 - 1968 2020
Single Work
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Controlled syntheses of Ag nanoparticles inside MOFs by using amine-boranes as vapour phase reductants Reviewed
Yohei Takashima, Yasushi Sato, Takaaki Tsuruoka, Kensuke Akamatsu
Dalton Transactions 2020
Joint Work
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Efficacy of Fermented Extract Obtained from Multiple-Processed Fermentation Technology of Thermophilic Bacteria and Yeast Reviewed
Daichi Kawano, Zhengzheng Liao, Jing Nie, Akira Date, Eduardo Perez, Jose Fernandez, Corey Webb, Kristen Huber, Jeffry B. Stock, Junji Kawakami, Zihua Fu
JOURNAL OF JAPANESE COSMETIC SCIENCE SOCIETY 44 ( 3 ) 194 - 202 2020
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Novel purification process for amyloid beta peptide(1-40) Invited Reviewed
Usui, K., Yokota, S.-I., Iwata, K., Hamada, Y.
Processes 8 2020
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Mass spectrometry-based solid phase peptide reaction assay for detecting allergenicity using an immobilized peptide-conjugating photo-cleavable linker Reviewed International journal
Miyazaki, H., Samejima, Y., Iwata, K., Minamino, Y., Hikida, S., Ariumi, H., Ikeda, H., Hamada, Y., Yamashita, K., Usui, K.
International Journal of Molecular Sciences 21 2020
Joint Work
Authorship：Last author, Corresponding author
The biological process of skin sensitization depends on the ability of a sensitizer to modify endogenous proteins. A direct peptide reactivity assay (DPRA), based on the biological process of skin sensitization, was developed as an alternative to controversial animal experiments. Although DPRA has been endorsed by industries and is internationally accepted as promising, it has several drawbacks, such as incompatibility with hydrophobic chemicals, inability to perform detailed reaction analysis, and ability to evaluate only single components. Here, we demonstrated that sensitizers and peptide adducts can be easily identified using a mass spectrometry-based solid-phase peptide reaction assay (M-SPRA). We synthesized peptides with a photo-cleavable linker immobilized on resins. We showed the potential of M-SPRA in predicting skin sensitization by measuring the peptide adducts that were selectively eluted from the resin after cleaving the linker post-reaction. M-SPRA provides more detailed information regarding chemical reactivity and accurate assessment of test samples, including mixtures. M-SPRA may be helpful for understanding the binding mechanism of sensitizers (toxicology), which may assist in further refining reactivity assays and aiding in the interpretation of reactivity data.
DOI： 10.3390/ijms21218332
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Synthesis of peptide-immobilized magnetic beads, and peptide reactivity assay for assessing skin sensitization utilizing chromophore Reviewed International journal
Miyazaki, H., Takaishi, H., Ikeda, H., Ariumi, H., Hamada, Y., Yamashita, K., Usui, K.
Processes 8 2020
Joint Work
Authorship：Last author, Corresponding author
DPRA (direct peptide reactivity assay) and ADRA (amino acid derivative reactivity assay), which are based on the biological events of skin sensitization, were developed as alternatives to the controversial animal experiments. These assays are described in the OECD (Organization for Economic Co-operation and Development) guideline, Test No. 442C. Although these assays have been endorsed by the industries and internationally accepted as promising and effective tests for in vitro skin sensitization, they suffer from several drawbacks, such as incompatibility with hydrophobic chemicals and complicated sample processing. Here, we demonstrated a chromophore-based solid phase peptide reaction assay in vitro using peptides immobilized on magnetic beads (C-SPRA-MB). We successfully synthesized lysine (Lys) and cysteine (Cys) immobilized on magnetic microbeads. However, Cys immobilized magnetic microbeads showed gradual decomposition of the magnetic beads due to SH oxidation. Using Lys immobilized magnetic microbeads, we demonstrated the capacity of C-SPRA-MB to predict skin sensitization by measuring free amino groups of the Lys after reaction with test chemicals. First, the free amines on the microbeads were reacted with bromophenol blue (BB). Then, by treatment with a saturated solution of Lys, the bound BBs were released and quantified. C-SPRA-MB provides high-throughput and accurate assays for assessments of chemicals, including with low-potency as skin sensitizers and poor water solubility. C-SPRA-MB may be useful for effective prediction of their skin sensitization potential in the process of compound screening, especially in the case of misclassified by DPRA and ADRA. Thus, C-SPRA-MB can be applied to assessing the sensitization potential of medical, pharmaceutical, cosmetics, and industrial compounds.
DOI： 10.3390/pr8101257
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Effect of tryptophan residues on gold mineralization by a gold reducing peptide Reviewed International journal
Ozaki, M., Yoshida, S., Oura, M., Tsuruoka, T., Usui, K.
RSC Advances 10 2020
Joint Work
Authorship：Last author, Corresponding author
AuBP1, obtained by phage display selection, was previously shown to produce gold nanoparticles without reducing agents. The tryptophan (Trp) residue located at the N-terminus of this peptide contributes to the reduction of Au3+ to Au0 and is involved in the nucleation and crystal growth of gold nanoparticles. However, clear guidelines for relationships between the number of Trp residues in the peptide and its gold reducing ability have not been established. We focused on gold mineralization and attempted to elucidate aspects of the underlying mechanism. We performed a detailed evaluation of the effects of modifying the N-terminus of the core sequence on gold mineralization without reducing agents. Besides, advantages of utilizing peptides in manufacturing gold nanoparticles are shown. UV-Vis measurements, TEM observations, and kinetic analyses were used to show that increasing the number of Trp residues in the peptide increases the reducing ability, causing predominance of the nucleation reaction and the production of small gold nanoparticles. In addition, these peptides also had the ability as a dispersant to protect the surface of gold nanoparticles. Furthermore, the catalytic activity of mineralized gold nanoparticles with peptides was higher than that of a commercial gold nanoparticle. This study should help to elucidate the relationship between peptide sequence and mineralization ability for use in materials chemistry.
DOI： 10.1039/d0ra07098j
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Bioinspired cell nuclear nanotransporters generated by self‐assembly of amphiphilic polysaccharide‐amino acid derivatives conjugates Reviewed
Koji Nagahama, Yoshinori Sano, Mitsuo Inui, Seika Aoyama, Tokitaka Katayama, Kimika Ono
Advanced Biosystems 4 1900189 2020
Joint Work
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美容医療 new horizon －幹細胞への期待－ Invited Reviewed
市橋正光、長濱宏治
日本香粧品学会誌 44 30 - 35 2020
Joint Work
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細胞移植用インジェクタブルゲルの開発 Invited Reviewed
長濱宏治
Precision Medicine 3 724 - 728 2020
Single Work