Papers -
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生命を制御するのは遺伝子か環境か－細胞内環境に左右される遺伝子の構造と機能－
三好大輔
高分子 55 ( 5 ) 322 - 325 2006.11
Single Work
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Effect of molecular crowding on DNA polymerase activity
三好大輔
Biotech. J., ( 1 ) 440 - 446 2006.11
Single Work
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Properties of long telomeric DNAs under cell-mimicking conditions
三好大輔
Nucleic Acids Symp. Ser. ( 50 ) 207 - 208 2006.11
Single Work
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The Suppression of Cell and Platelet Adhesion to Star-Shaped Poly(ethylene glycol)-Poly(L-lacitde) Block Copolymer Film, Reviewed
K. Nagahama, Y. Ohya, T. Ouchi,
Macromol. Biosci. 6 ( 6 ) 412 - 419 2006.11
Joint Work
Authorship：Lead author
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Synthesis of Star-Shaped 8arms Poly(ethylene glycol)-Poly(L-lacitde) Block Copolymer and Physical Properties of Its Solution Cast Film as Soft Biomaterial Reviewed
K. Nagahama, Y. Ohya, T. Ouchi,
Polym. J., 38 ( 8 ) 852 - 860 2006.11
Joint Work
Authorship：Lead author
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The roles of cosolutes on the hammerhead ribozyme activity
中野 修一
Nucleic Acids Symp. Ser. 50 81 - 82 2006.11
Joint Work
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The effect of the structure of cosolutes on the DNA duplex formation
中野 修一
Nucleic Acids Symp. Ser. 50 205 - 206 2006.11
Joint Work
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Recognition of the base pair-mimic nucleosides by DNA polymerases
中野 修一
Nucleic Acids Symp. Ser. 50 201 - 202 2006.11
Joint Work
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Protein-fingerprint data mining of a designed alpha-helical peptide array Reviewed
Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara
MOLECULAR BIOSYSTEMS 2 ( 9 ) 417 - 420 2006.9
Publisher：ROYAL SOC CHEMISTRY
The data generated from protein fingerprints with an a-helical peptide array were analyzed using several statistical methods such as hierarchical clustering analysis and principal component analysis to discriminate target proteins. The data generated from protein fingerprints with an a-helical peptide array were analyzed using several statistical methods such as hierarchical clustering analysis and principal component analysis to discriminate target proteins.
DOI： 10.1039/b608875a
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A novel stable RNA pentaloop that interacts specifically with a motif peptide of lambda-N protein（共著） Reviewed
J. Kawakami, H. Tokitoh, Y. Tanabe, and N. Sugimoto
Nucleoside, Nucleotides and Nucleic Acids 25 397 - 416 2006.4
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The negative regulation of p53 by hedgehog signaling Reviewed
Abe Yoshinori, Oda-Sato Eri, Tobiume Kei, Kawauchi Keiko, Okamoto Koji, Taya Yoichi, Tanaka Nobuyuki
CANCER RESEARCH 66 ( 8 ) 2006.4
Joint Work
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A novel peptide microarray for protein detection and analysis utilizing a dry peptide array system Reviewed
K Usui, K Tomizaki, T Ohyama, K Nokihara, H Mihara
MOLECULAR BIOSYSTEMS 2 ( 2 ) 113 - 121 2006.2
Publisher：ROYAL SOC CHEMISTRY
A novel dry peptide microarray system has been constructed that affords a practical solution for protein detection and analysis. This system is an array preparation and assay procedure tinder dry conditions that uses designed peptides as non-immobilized capture agents for the detection of proteins. The system has several advantages that include its portability and ease-Of-Use, as well as the fact that vaporization of sample solutions need not be considered. In this study, various proteins have been characterized with an a-helical peptide mini-library. When proteins were added to the peptide library array, the fluorescent peptides showed different fluorescent intensities depending on their sequences. The patterns of these responses could be regarded as protein fingerprints' (PFPs), which are sufficient to establish the identities of the target proteins. Furthermore, statistical analysis of the resulting PFPs was performed using cluster analysis. The PFPs of the proteins were clustered successfully depending on their families and binding properties. Additionally, the target protein was characterized using a nanolitre system and could be detected down to 1.2 fmol. These Studies imply that the dry peptide array system is a promising too] for detecting and analyzing target proteins. The dry peptide array will play a role in development of high-throughput protein-detecting nano/micro arrays for proteomics and ligand screening studies.
DOI： 10.1039/b514263r
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A PNA-DNA hybridization chip approach for the detection of beta-secretase activity Reviewed
S Sano, KY Tomizaki, K Usui, H Mihara
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 16 ( 3 ) 503 - 506 2006.2
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
Developed was the addressable chip technology based on the PNA-DNA complementary hybridization equipped with short seven-mer PNA-encoded peptides that can be a versatile scaffold to monitor on-chip immunoassays. We also developed and validated a methodology to perform beta-secretase enzyme assay with a highly sensitive fashion, resulting that a peptide substrate tethering dual fluorescent probes allowed us to detect beta-secretase activity 10 times more sensitively than assays in solution. (c) 2005 Elsevier Ltd. All rights reserved.
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A DNA duplex with extremely enhanced thermal stability based on controlled immobilization on gold nanoparticles Reviewed
K. Akamatsu, M. Kimura, Y. Shibata, S. Nakano, D. Miyoshi, H. Nawafune, and N. Sugimoto
Nano Lett. 3 491 - 495 2006.1
Joint Work
DOI： 10.1021/nl0524748
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Use of the polymorphic nature of high-order DNA structures for the design of dynamic and functional nanodevices Invited Reviewed
D. Miyoshi, N. Sugimoto
Recent Devel. Nucleic Acids Res. 2 49 - 70 2006.1
Joint Work
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Use of the polymorphic nature of high-order DNA structures for the design of dynamic and functional nanodevices
D. Miyoshi, N. Sugimoto
Recent Devel. Nucleic Acids Res. 2 49 - 70 2006.1
Joint Work
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Photodimerization of Anthryl Moieties in a Poly(methacrylic acid) Derivative as Reversible Cross-linking Step in Molecular Imprinting
J. Matsui, Y. Ochi, K. Tamaki
Chem. Lett. 80 - 81 2006
Joint Work
Authorship：Lead author
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Molecularly-Imprinted Polymeric Logic Gates Selective for Predetermined Chemical Input Species
松井淳
Chem. Comm. 3217 - 3219 2006
Single Work
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Dynamic redistribution of vasa homolog and exclusion of somatic cell determinants during germ cell specification in Ciona intestinalis.
西方敬人
Development ( 133 ) 2683 - 2693 2006
Single Work
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UV-irradiated cells as a screening system for the antioxidants.
西方敬人
Proceedings of the 13th Biennial Congress of the International Society for Free Radical Research -SFRR 253 - 256 2006
Single Work