Papers -
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Sobuj Shahidul Islam,Tatsuya Dode,Soma Kawashima,Myu Fukuoka,Takaaki Tsuruoka andKoji Nagahama
Gels 11 ( 7 ) 514 2025.7
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Comprehensive analysis of stability and variability of DNA minimal i-motif structures Invited Reviewed
K. Ashida, A. Kitabayashi, K. Nishiyama, and S. Nakano
Molecules 30 1831 2025.4
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One-Pot Synthesis and Immobilization of Gold Nanoparticles Using Peptidyl Microbeads
Shuhei Yoshida, Koki Yoshida, Taichi Isozaki, Maho Oura, Makoto Ozaki, Takaaki Tsuruoka, Kenji Usui
Molecules 30 1689 2025.4
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Liquid-liquid phase separation induced by i-motif DNA under molecular crowding conditions Invited Reviewed
Ryosuke Suzuki, Mitsuki Tsuruta, Sumit Shil, Kosei Morohashi, Keiko Kawauchi, Daisuke Miyoshia
Polymer J. 2025.4
Authorship：Corresponding author
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Tendon Tissue Regeneration With Cell Orientation Using an Injectable Alginate-Cell Cross-linked Gel Reviewed
Jun Yamaguchi, Kentaro Homan, Tomohiro Onodera, Masatake Matsuoka, Shoutaro Arakawa, Natsumi Ueda, Nana Kawate, Takayuki Nonoyama, Yoshinori Katsuyama, Koji Nagahama, Mitsuru Saito, Norimasa Iwasaki
The American Journal of Sports Medicine 2025.3
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Three- and four-stranded nucleic acid structures and their ligands Invited Reviewed
Yoshiki Hashimoto, Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, and Daisuke Miyoshi
RSC Chemical Biology 6 466 - 491 2025.3
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ケミカルバイオロジーの冒険（１２） 核酸を標的とした低分子・中分子化合物 Invited
堂野主税、勝田陽介、三好大輔
現代化学 648 32 - 39 2025.2
Authorship：Last author
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Roles of Loop Region in Folding Kinetics and Transcription Inhibition of DNA G-Quadruplexes Reviewed
64 609 - 619 2025.2
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Development of a New Structural Transformation Reaction in Cr-Based MOFs Induced by Fluoride Reviewed
Shintaro Tanaka, Ryoya Miyata, Reon Teramoto, Junya Sugimoto, Takaaki Tsuruoka, Kensuke Akamatsu, Yohei Takashima
Chem. Asian J e202401717 2025.2
Authorship：Last author, Corresponding author
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Development of detection system for lead ions in mixture solutions using UV-Vis measurements with peptide immobilized microbeads
Shuhei Yoshida, Koki Yoshida, Yoshio Hamada, Takaaki Tsuruoka, Kenji Usui
Scientific Reports 15 3249 2025.1
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Complex and Non-sequential Current Signatures of a β-Hairpin Peptide Confined in a Nanopore International coauthorship
Misa Yamaji, Mauro Chinappi, Blasco Morozzo della Rocca, Kenji Usui, Ryuji Kawano
Analytical Chemistry 97 2044 - 2051 2025.1
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Roles of Loop Region in Folding Kinetics and Transcription Inhibition of DNA G-Quadruplexes Reviewed
Minori Nakata, Naoki Kosaka, Keiko Kawauchi, Daisuke Miyoshi
Biochemistry 64 609 - 619 2025.1
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Factors affecting liquid-liquid phase separation of RGG peptides with DNA G-Quadruplex Reviewed
Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, Daisuke Miyoshi
ChemMedChem 20 ( 2 ) e202400460 2025.1
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The role of cytosine methylation in regulating the topology and liquid-liquid phase separation of DNA G-quadruplexes Reviewed
Mitsuki Tsuruta, Sumit Shil, Shinya Taniguchi, Keiko Kawauchi, Daisuke Miyoshi
Chemical Science 16 ( 10 ) 4213 - 4225 2025.1
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Sequence-Specific Free Energy Changes in DNA/RNA Induced by a Single LNA-T Modification in Antisense Oligonucleotides Reviewed
Elisa Tomita-Sudo, Tomoka Akita, Nae Sakimoto, Saori Tahara-Takamine, Junji Kawakami
International Journal of Molecular Sciences 25 13240 - 13240 2024.12
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Bioinformatic analysis of actin-binding proteins in the nucleolus during heat shock Invited Reviewed
Shinya Taniguchi, Takeru Torii, Toshiyuki Goto, Kohei Takeuchi, Rine Katsumi, Mako Sumida, Sunmin Lee, Wataru Sugimoto, Masaya Gessho, Katsuhiko Itoh, Hiroaki Hirata, Junji Kawakami, Daisuke Miyoshi, Keiko Kawauchi
Genes 15 1580 2024.12
Authorship：Last author, Corresponding author
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L-Histidine Modulates the Catalytic Activity and Conformational Changes of the HD3 Deoxyribozyme Reviewed
Nae Sakimoto, Hirofumi Imanaka, Elisa Tomita-Sudo, Tomoka Akita, Junji Kawakami
genes 15 1481 2024.11
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NRAS DNA G-quadruplex-targeting molecules for sequence-selective enzyme inhibition Reviewed
Yoshiki Hashimoto, Hiroki Kubo, Keiko Kawauchi, Daisuke Miyoshi
Chem. Commun. 60 13179 - 13182 2024.11
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Detection of Conformational Changes of G4 Aptamer in the Presence of Different Cations using Calorimetry Invited Reviewed
Elisa Tomita-Sudo, Taku Ishigaki, Masako Hirose, Maki Kato, and Junji Kawakami
Netsu Sokutei 51 ( 4 ) 148 - 152 2024.10
Authorship：Last author
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NRAS DNA G-quadruplex-targeting molecules for sequence-selective enzyme inhibition Invited Reviewed
Yoshiki Hashimoto, Hiroki Kubo, Keiko Kawauchi and Daisuke Miyoshi
Chem. Commun. 2024.10
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The role of cytosine methylation in regulating the topology and liquid-liquid phase separation of DNA G-quadruplexes Reviewed
Mitsuki Tsuruta, Sumit Shil, Shinya Taniguchi, Keiko Kawauchi and Daisuke Miyoshi
Chem. Sci. 16 4213 - 4225 2024.10
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Structural transformation of silver(I)-thiolate coordination plymer film at solid-liquid interfaces Reviewed International coauthorship
Myu Fukuoka, Yohei Takashima, Kensuke Akamatsu, Aude Demessence, Takaaki Tsuruoka
CrystEngComm 26 6269 - 6273 2024.10
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Factors Affecting Liquid-Liquid Phase Separation of RGG Peptides with DNA G-Quadruplex Invited Reviewed
Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, Daisuke Miyoshi
ChemMedChem 2024.9
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Bioinformatic Analysis of Actin-Binding Proteins in the Nucleolus During Heat Shock Invited Reviewed
Shinya Taniguchi, Takeru Torii ,Toshiyuki Goto, Kohei Takeuchi, Rine Katsumi, Mako Sumida, Sunmin Lee, Wataru Sugimoto, Masaya Gessho, Katsuhiko Itoh, Hiroaki Hirata, Junji Kawakami, Daisuke Miyoshi and Keiko Kawauchi
Genes 15 ( 12 ) 1580 2024.9
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Non-canonical olfactory pathway activation induces cell fusion of cervical cancer cells. Reviewed International journal
Keigo Araki, Takeru Torii, Kohei Takeuchi, Natsuki Kinoshita, Ryoto Urano, Rinka Nakajima, Yaxuan Zhou, Tokuo Kobayashi, Tadayoshi Hanyu, Kiyoshi Ohtani, Kimiharu Ambe, Keiko Kawauchi
Neoplasia 57 101044 - 101044 2024.8
Authorship：Last author
Multinucleation occurs in various types of advanced cancers and contributes to their malignant characteristics, including anticancer drug resistance. Therefore, inhibiting multinucleation can improve cancer prognosis; however, the molecular mechanisms underlying multinucleation remain elusive. Here, we introduced a genetic mutation in cervical cancer cells to induce cell fusion-mediated multinucleation. The olfactory receptor OR1N2 was heterozygously mutated in these fused cells; the same OR1N2 mutation was detected in multinucleated cells from clinical cervical cancer specimens. The mutation-induced structural change in the OR1N2 protein activated protein kinase A (PKA), which, in turn, mediated the non-canonical olfactory pathway. PKA phosphorylated and activated furin protease, resulting in the cleavage of the fusogenic protein syncytin-1. Because this cleaved form of syncytin-1, processed by furin, participates in cell fusion, furin inhibitors could suppress multinucleation and reduce surviving cell numbers after anticancer drug treatment. The improved anticancer drug efficacy indicates a promising therapeutic approach for advanced cervical cancers.
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Quantitative Effects of the Loop Region on Topology, Thermodynamics, and Cation Binding of DNA G-quadruplexes Reviewed
ACS Omega 9 35028 - 35036 2024.7
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Development of a CaCO3 Precipitation Method Using a Peptide and Microwaves Generated by a Magnetron Reviewed
Fumihiro Kayamori, Hiroyuki Togashi, Natsumi Endo, Makoto Ozaki, Kan Hirao, Yonejiro Arimoto, Ryuji Osawa, Takaaki Tsuruoka, Takahito Imai, Kin-ya Tomizaki, Tomohiro Umetani, Nobuhiro Nakanishi, Kenji Usui
Processes 12 1327 - 1327 2024.6
Publisher：MDPI AG
Microwave applications, such as microwave ovens and mobile phones, are ubiquitous and indispensable in modern society. As the utilization of microwave technology is becoming more widespread, the effects of microwaves on living organisms and physiological processes have received increased attention. This study aimed to investigate the effects of microwaves on calcium carbonate biomineralization as a model biochemical process. A magnetron oscillator was used to generate 2450 MHz microwaves because magnetrons are relatively inexpensive and widespread. We conducted transmission electron microscopy (TEM), atomic force microscopy (AFM), TEM-electron energy-loss spectroscopy (EELS), dynamic light scattering (DLS), and high-performance liquid chromatography (HPLC) measurements to analyze the calcium carbonate precipitates. Our findings showed the formation of string-like precipitates of calcium carbonate upon microwave irradiation from one direction, similar to those obtained using a semiconductor oscillator, as reported previously. This implied that the distribution of the frequency had little effect on the morphology. Furthermore, spherical precipitates were obtained upon microwave irradiation from two directions, indicating that the morphology could be controlled by varying the direction of microwave irradiation. Magnetrons are versatile and also used in large-scale production; thus, this method has potential in medical and industrial applications.
DOI： 10.3390/pr12071327
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Development of a CaCO3 Precipitation Method Using a Peptide and Microwaves Generated by a Magnetron Reviewed
Fumihiro Kayamori Hiroyuki Togashi Natsumi Endo Makoto Ozaki Kan Hirao Yonejiro Arimoto Ryuji Osawa Takaaki Tsuruoka Takahito Imai Kin-ya Tomizaki Tomohiro Umetani Nobuhiro Nakanishi Kenji Usui
Processes 12 ( 7 ) 1327 2024.6
Microwave applications, such as microwave ovens and mobile phones, are ubiquitous and indispensable in modern society. As the utilization of microwave technology is becoming more widespread, the effects of microwaves on living organisms and physiological processes have received increased attention. This study aimed to investigate the effects of microwaves on calcium carbonate biomineralization as a model biochemical process. A magnetron oscillator was used to generate 2450 MHz microwaves because magnetrons are relatively inexpensive and widespread. We conducted transmission electron microscopy (TEM), atomic force microscopy (AFM), TEM-electron energy-loss spectroscopy (EELS), dynamic light scattering (DLS), and high-performance liquid chromatography (HPLC) measurements to analyze the calcium carbonate precipitates. Our findings showed the formation of string-like precipitates of calcium carbonate upon microwave irradiation from one direction, similar to those obtained using a semiconductor oscillator, as reported previously. This implied that the distribution of the frequency had little effect on the morphology. Furthermore, spherical precipitates were obtained upon microwave irradiation from two directions, indicating that the morphology could be controlled by varying the direction of microwave irradiation. Magnetrons are versatile and also used in large-scale production; thus, this method has potential in medical and industrial applications.
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Inhibition of RNA phosphodiester backbone cleavage in the presence of organic cations of different sizes Invited Reviewed International journal
S. Yoshioka, A. Doi, and S. Nakano
ChemBioChem 25 ( 13 ) e202400276 2024.5
Joint Work
Authorship：Last author, Corresponding author Publisher：Wiley
Living cells contain various types of organic cations that may interact with nucleic acids. In order to understand the nucleic acid–binding properties of organic cations of different sizes, we investigated the ability of simple organic cations to inhibit the RNA phosphodiester bond cleavage promoted by Mg2+, Pb2+, and RNA-cleaving serum proteins. Kinetic analysis using chimeric DNA–RNA oligonucleotides showed that the cleavage at ribonucleotide sites was inhibited in the presence of monovalent cations comprising alkyl chains or benzene rings. The comparison of the cleavage rates in the presence of quaternary ammonium and phosphonium ions indicated that the steric hindrance effect of organic cations on their binding to the RNA backbone is significant when the cation size is larger than the phosphate–phosphate distance of a single-stranded nucleic acid. The cleavage inhibition was also observed for ribonucleotides located in long loops but not in short loops of oligonucleotide structures, indicating less efficient binding of bulky cations to structurally constrained regions. These results reveal the unique nucleic acid–binding properties of bulky cations distinct from those of metal ions.
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Interfacial analysis of the ion-transport process controlling the steady-state current in a two-phase electrodeposition system using polyelectrolyte membranes Reviewed
Shunsuke Yamada, Yohei Takashima, Takaaki Tsuruoka, Kensuke Akamatsu
RSC Appl. Interfaces 1 1069 - 1076 2024.5
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Separation of the diastereomers of phosphorothioated siRNAs by anion-exchange chromatography under non-denaturing conditions Reviewed International journal
Hiroyuki Togawa, Takashi Okubo, Kazuki Horiuchi, Takao Yamaguchi, Elisa Tomita-Sudo, Tomoka Akita, Junji Kawakami, Satoshi Obika
Journal of Chromatography A 1721 464847 2024.4
Joint Work
In recent years, several small interfering RNA (siRNA) therapeutics have been approved, and most of them are phosphorothioate (PS)-modified for improving nuclease resistance. This chemical modification induces chirality in the phosphorus atom, leading to the formation of diastereomers. Recent studies have revealed that Sp and Rp configurations of PS modifications of siRNAs have different biological properties, such as nuclease resistance and RNA-induced silencing complex (RISC) loading. These results highlight the importance of determining diastereomeric distribution in quality control. Although various analytical approaches have been used to separate diastereomers (mainly single-stranded oligonucleotides), it becomes more difficult to separate all of them as the number of PS modifications increases. Despite siRNA exhibits efficacy in the double-stranded form, few reports have examined the separation of diastereomers in the double-stranded form. In this study, we investigated the applicability of non-denaturing anion-exchange chromatography (AEX) for the separation of PS-modified siRNA diastereomers. Separation of the four isomers of the two PS bonds tended to improve in the double-stranded form compared to the single-stranded form. In addition, the effects of the analytical conditions and PS-modified position on the separation were evaluated. Moreover, the elution order of the Sp and Rp configurations was confirmed, and the steric difference between them, i.e., the direction of the anionic sulfur atom, appeared to be important for the separation mechanism in non-denaturing AEX. Consequently, all 16 peak tops of the four PS modifications were detected in one sequence, and approximately 30 peak tops were detected out of 64 isomers of six PS bonds, indicating that non-denaturing AEX is a useful technique for the quality control of PS-modified siRNA therapeutics.
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細胞内環境で安定化する核酸構造を標的にした低分子薬の開発 Invited
橋本 佳樹, 川内 敬子, 三好 大輔
MEDCHEM NEWS (日本薬学会） 34 ( 1 ) 36 - 42 2024.4
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Basic protein- and peptide-induced stabilization of long-loop DNA G-quadruplexes Reviewed International journal
K. Tanabe, K. Miyazaki, H. Umeno, M. Takemoto, and S. Nakano
Biochimie 219 110 - 117 2024.4
Joint Work
Authorship：Last author, Corresponding author Publisher：Elsevier
The human genome contains many G-quadruplex-forming sequences, including sequences containing long single-stranded loops that are believed to be unfavorable for G-quadruplex formation. The intracellular environment of biological cells is crowded with proteins with charged surfaces. Understanding the effects of protein-rich environments is important for understanding the formation of G-quadruplexes in an intracellular environment. In this study, we investigated the structural stability of DNA G-quadruplexes in the presence of several types of globular proteins (lysozyme, cytochrome c, bovine serum albumin, myoglobin, histone proteins, and serum proteins), unstructured polypeptides (protamine and poly-l-lysine), and oligopeptides (RGG/RG-domain peptides and short repeated peptides). Thermal melting studies of G-quadruplex-forming oligonucleotides derived from the human telomeric repeat sequence revealed that environments containing high concentrations of proteins and peptides differently affected the G-quadruplex stability according to their loop lengths. We found that weak electrostatic interactions of G-quadruplex loops with basic proteins and peptides improved the stability of long-loop G-quadruplexes and the interactions were strengthened under crowded conditions simulated by dextran. The comparison of the effects of different types of proteins and peptides indicated that excluded volume interactions and structural flexibility of both DNA and polypeptide chains influenced the efficiency of their interactions. This study provides insights into long-loop G-quadruplex stability in a crowded intracellular environment and the recognition of G-quadruplexes by arginine-rich domains of G-quadruplex-binding proteins.
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Hisae Tateishi-Karimata, Keiko Kawauchi, Shuntaro Takahashi, Naoki Sugimoto
Journal of the American Chemical Society 146 ( 12 ) 8005 - 8015 2024.3
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Yohei Takashima, Naoto Tanabe, Shintaro Tanaka, Takaaki Tsuruoka, Kensuke Akamatsu
Crystal Growth & Design 24 ( 4 ) 1766 - 1773 2024.2
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Cr(NO3)3 as a new etching reagent for an Al-based metal-organic framework to control its crystal size and defects Reviewed
Yohei Takashima, Naoto Tanabe, Shintaro Tanaka, Takaaki Tsuruoka, Kensuke Akamatsu
Cryst. Growth Des. 24 1766 - 1773 2024.2
Authorship：Lead author, Corresponding author
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Structure and thermoelectric characterization of p-Type SnTe nanobulk material synthesized by charge compensation chemical reaction Reviewed
Ryosuke Fujiwara, Yuta Ikeda, Takuto Kawaguchi, Yohei Takashima, Takaaki Tsuruoka, Kensuke Akamatsu
Energies 17 190 2024.2
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Inhibitory Effects of Shikonin Dispersion, an Extract of Lithospermum erythrorhizon Encapsulated in β-1,3-1,6 Glucan, on Streptococcus mutans and Non-Mutans Streptococci Reviewed
Ryota Nomura, Yuto Suehiro, Fumikazu Tojo, Saaya Matayoshi, Rena Okawa, Masakazu Hamada, Shuhei Naka, Michiyo Matsumoto-Nakano, Rika Unesaki, Kazuya Koumoto, Keiko Kawauchi, Takahito Nishikata, Tatsuya Akitomo, Chieko Mitsuhata, Masatoshi Yagi, Toshiro Mizoguchi, Koki Fujikawa, Taizo Taniguchi and Kazuhiko Nakano
25 1075 2024.1
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Inhibitory Effects of Shikonin Dispersion, an Extract of Lithospermum erythrorhizon Encapsulated in β -1,3-1,6 Glucan, on Streptococcus mutans and Non-Mutans Streptococci Reviewed
Nomura R, Suehiro Y, Tojo F, Matayoshi S, Okawa R, Hamada M, Naka S, Matsumoto-Nakano M, Unesaki R, Koumoto K, Kawauchi K, Nishikata T, Akitomo T, Mitsuhata C, Yagi M, Mizoguchi T, Fujikawa K, Taniguchi T, Nakano K
International Journal of Molecular Science 25 1075 2024.1
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Inhibitory Effects of Shikonin Dispersion, an Extract of Lithospermum erythrorhizon Encapsulated in β-1,3-1,6 Glucan, on Streptococcus mutans and Non-Mutans Streptococci. Reviewed International journal
Ryota Nomura, Yuto Suehiro, Fumikazu Tojo, Saaya Matayoshi, Rena Okawa, Masakazu Hamada, Shuhei Naka, Michiyo Matsumoto-Nakano, Rika Unesaki, Kazuya Koumoto, Keiko Kawauchi, Takahito Nishikata, Tatsuya Akitomo, Chieko Mitsuhata, Masatoshi Yagi, Toshiro Mizoguchi, Koki Fujikawa, Taizo Taniguchi, Kazuhiko Nakano
International journal of molecular sciences 25 ( 2 ) 2024.1
Shikonin is extracted from the roots of Lithospermum erythrorhizon, and shikonin extracts have been shown to have inhibitory effects on several bacteria. However, shikonin extracts are difficult to formulate because of their poor water solubility. In the present study, we prepared a shikonin dispersion, which was solubilized by the inclusion of β-1,3-1,6 glucan, and analysed the inhibitory effects of this dispersion on Streptococcus mutans and non-mutans streptococci. The shikonin dispersion showed pronounced anti-S. mutans activity, and inhibited growth of and biofilm formation by this bacterium. The shikonin dispersion also showed antimicrobial and antiproliferative effects against non-mutans streptococci. In addition, a clinical trial was conducted in which 20 subjects were asked to brush their teeth for 1 week using either shikonin dispersion-containing or non-containing toothpaste, respectively. The shikonin-containing toothpaste decreased the number of S. mutans in the oral cavity, while no such effect was observed after the use of the shikonin-free toothpaste. These results suggest that shikonin dispersion has an inhibitory effect on S. mutans and non-mutans streptococci, and toothpaste containing shikonin dispersion may be effective in preventing dental caries.
DOI： 10.3390/ijms25021075
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Direct Formation of ZIF-8 Crystal Thin Films on the Surface of a Zinc Ion-Doped Polymer Substrate Reviewed
Takaaki Tsuruoka, Kaito Araki, Kouga Kawauchi, Yohei Takashima, Kensuke Akamatsu
Inorganics 12 ( 1 ) 21 - 21 2024.1
Authorship：Lead author, Corresponding author Publisher：MDPI AG
Thin films of metal–organic frameworks (MOFs) on polymer substrates and MOF/polymer mixed-matrix membranes play crucial roles in advancing the field of gas separation membranes. In this paper, we present a novel method for the direct formation of continuous ZIF-8 crystal films on a polymer substrate doped with Zn²+. Our approach involves ion exchange between the doped zinc ions within the substrate and sodium ions in the presence of a CH3COONa additive, as well as interfacial complexation with eluted zinc ions and 2-methylimidazole (2-MeIM). The key factors affecting the formation of ZIF-8 crystals on the substrate were the concentrations of CH3COONa and 2-MeIM. A time-course analysis revealed that the nucleation rate during the early stages of the reaction significantly affected the surface morphology of the resulting ZIF-8 crystal films. Specifically, a higher nucleation rate led to the formation of continuous small ZIF-8 crystal films. This innovative approach enables the fabrication of densely packed, uniform ZIF-8 crystal films.
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Direct formation of ZIF-8 crystal thin films on the surface of a zinc ion-doped polymer substrate Reviewed
Takaaki Tsuruoka, Kaito Araki, Kouga Kawauchi, Yohei Takashima, Kensuke Akamatsu
Inorganics 12 21 2024.1
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“Interfacial analysis of the ion-transport process controlling the steady-state current in a two-phase electrodeposition system using polyelectrolyte membranes
S. Yamada, Y. Takashima, T. Tsuruoka, K. Akamatsu
RSC Applied Interfaces 2024
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Structural transformation of silver(I)-thiolate coordination polymer film at solid–liquid interfaces
M. Fukuoka, Y. Takashima, K. Akamatsu, A. Demessence, T. Tsuruoka
CrystEngComm 26 6269 - 6273 2024
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Preparation of golf ball-like polymer particles bearing site-selective functional groups
T. Morishita, I. Maitani, T. Michiura, N. Suzuki, T. Suzuki, K. Akamatsu, H. Minami
ACS Appl. Mater.Interfaces in press 2024
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Structure and Thermoelectric Characterization of p-Type SnTe Nanobulk Material Synthesized by Charge Compensation Chemical Reaction
Ryosuke Fujiwara, Yuta Ikeda, Takuto Kawaguchi, Yohei Takashima , Takaaki Tsuruoka and Kensuke Akamatsu
Energies 17 ( 190 ) 2024
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Cr(NO3)3 as a New Etching Reagent for an Al-based Metal-Organic Framework to Control Its Crystal Size and Defects
Y. Takashima, N. Tanabe, S. Tanaka, T. Tsuruoka, K. Akamatsu
Cryst. Growth Des 24 1766 - 1773 2024
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Direct Formation of ZIF-8 Crystal Thin Films on the Surface of a Zinc Ion-Doped Polymer Substrate
T. Tsuruoka, K. Araki, K. Kawauchi, Y. Takashima, K. Akamatsu
Inroganics 12 ( 21 ) 2024
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Shunsuke Yamada, Yohei Takashima, Takaaki Tsuruoka, Kensuke Akamatsu
RSC Applied Interfaces 2024
Publisher：Royal Society of Chemistry (RSC)
An interfacial model has been developed for describing ion transport kinetics at the membrane/solution interface in the new solid-electrodeposition system.
DOI： 10.1039/d4lf00112e
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Asuka Nishibe, Ryohei Akiyoshi, Akinori Saeki, Kazuyoshi Ogasawara, Takaaki Tsuruoka, Daisuke Tanaka
Chemical Communications 2024
Publisher：Royal Society of Chemistry (RSC)
The impact of inorganic (–Cd–S–)<sub>n</sub> assemblies on semiconductive properties is demonstrated by systematically synthesizing one-dimensional Cd(ii) benzenethiolate coordination polymers.
DOI： 10.1039/d3cc05689a
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Ryosuke Fujiwara, Yuta Ikeda, Takuto Kawaguchi, Yohei Takashima, Takaaki Tsuruoka, Kensuke Akamatsu
Energies 17 ( 1 ) 190 - 190 2023.12
Publisher：MDPI AG
SnTe is the most widely studied p-type thermoelectric (TE) alternative to PbTe. In this study, we prepared a nanostructured SnTe bulk material via spark plasma sintering from a precursor synthesized by a chemical precipitation process without using organic molecules. The sintered sample comprised tiny grains (100–300 nm) with high-density grain boundaries. Eventually, because the material would contain no impurities acting as scattering nodes of charge carriers, the material exhibited a relatively high electrical conductivity of 7.07 × 105 Sm−1 at 310 K. The material demonstrated low lattice thermal conductivity (0.87 Wm−1K−1 at 764 K), which can be owing to the increasing phonon scattering at grain boundaries. The maximum ZT was 0.31 at 764 K in the measured temperature range. This study provides a method for the design of phase-pure and surfactant-free SnTe thermoelectric materials that exhibit low lattice thermal conductivity and high carrier mobility using a chemical synthetic approach.
DOI： 10.3390/en17010190
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Loss of p53 function promotes DNA damage-induced formation of nuclear actin filaments Reviewed
Torii T, Sugimoto W, Itoh K, Kinoshita N, Gessho M, Goto T, Uehara I, Nakajima W, Budirahardja Y, Miyoshi D, Nishikata T, Tanaka N, Kawauchi K
Cell Death and Disease 14 766 2023.11
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Comparison of evolutionary relationships between Branchiostoma floridae, Ciona intestinalis, and Homo sapiens globins provide evidence of gene co-option and convergent evolution Reviewed
Yano N, Minamoto T, Yamaguchi H, Goto T, Nishikata T
International Journal of Molecular Science 24 16009 2023.11
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Sequencing of Morpholino Antisense Oligonucleotides Using Electron Capture Dissociation Mass Spectrometry Reviewed International journal
Kaoru Karasawa, Eva Duchoslav, Lyle Burton, Junji Kawakami, Takashi Baba
Analytical Chemistry 95 ( 44 ) 16352 - 16358 2023.10
Joint Work
We report the first sequencing of morpholino antisense oligonucleotides (phosphorodiamidate morpholino oligomers, PMOs) using electron capture dissociation (ECD) mass spectrometry. In this research, we found dissociation of the backbone of 18- to 25-mer PMOs to produce d and z ions as the major ions, and 100% cleavage coverage (sequence coverage) was obtained with these ions. This is a critical contrast with beam-type collision-induced dissociation, which dominantly induces base loss, so it is difficult to obtain sequence information. The results showed that an electron beam energy (typically 15 eV) can be used universally for PMOs with different sequences, lengths, and charge states so that no detailed optimization is required for multiprecursor targeting liquid chromatography coupled with tandem mass spectrometry measurements. We also confirmed that the ECD reaction speed was compatible with the high-performance liquid chromatography time scale. Finally, we demonstrated a liquid chromatography electron capture dissociation tandem mass spectrometry workflow to survey the modification sites of the emulated PMO impurities.
DOI： 10.1021/acs.analchem.3c03621
DOI： 10.1021/acs.analchem.3c03621
Other Link： https://doi.org/10.1021/acs.analchem.3c03621
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Mitochondrial Dynamics of Bcl-2 Family Proteins during 17-β-Estradiol-Induced Apoptosis Correlate with the Malignancy of Endometrial Cancer Cells Reviewed
Takahiro Yaguchi, Misaki Kameno, Hirofumi Taira, and Junji Kawakami
Biochemstry 62 ( 21 ) 3041 - 3049 2023.10
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Comparison of Analysis Data of Model Oligonucleotide Therapeutics Obtained with Different Types of Liquid Chromatograph- Mass Spectrometers Reviewed
Kenji Hirose, Tokuyuki Yoshida, Maki Terasaki, Hiroshi Sezaki, Kaoru Karasawa, Noriyuki Iwasaki, Kentaro Takahara, Naomi Takiguchi, Mitsuaki Sekiguchi, Hirokazu Nankai, Emi Saito, Hideaki Sato, Takashi Osawa, Takao Yamaguchi, Kosuke Ito, Junji Kawakami, Satoshi Obika, and Takao Inoue
Pharmaceutical and Medical Device Regulatory Science 54 439 - 454 2023.10
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Loss of p53 function promotes DNA damage-induced formation of nuclear actin filaments Reviewed International journal
Takeru Torii, Wataru Sugimoto, Katsuhiko Itoh, Natsuki Kinoshita, Masaya Gessho, Toshiyuki Goto, Ikuno Uehara, Wataru Nakajima, Yemima Budirahardja, Daisuke Miyoshi, Takahito Nishikata, Nobuyuki Tanaka, Hiroaki Hirata, Keiko Kawauchi
Cell Death & Disease 14 ( 11 ) 766766 - 766 2023.8
Tumor suppressor p53 plays a central role in response to DNA damage. DNA-damaging agents modulate nuclear actin dynamics, influencing cell behaviors; however, whether p53 affects the formation of nuclear actin filaments remains unclear. In this study, we found that p53 depletion promoted the formation of nuclear actin filaments in response to DNA-damaging agents, such as doxorubicin (DOXO) and etoposide (VP16). Even though the genetic probes used for the detection of nuclear actin filaments exerted a promotive effect on actin polymerization, the detected formation of nuclear actin filaments was highly dependent on both p53 depletion and DNA damage. Whilst active p53 is known to promote caspase-1 expression, the overexpression of caspase-1 reduced DNA damage-induced formation of nuclear actin filaments in p53-depleted cells. In contrast, co-treatment with DOXO and the pan-caspase inhibitor Q-VD-OPh or the caspase-1 inhibitor Z-YVAD-FMK induced the formation of nuclear actin filament formation even in cells bearing wild-type p53. These results suggest that the p53-caspase-1 axis suppresses DNA damage-induced formation of nuclear actin filaments. In addition, we found that the expression of nLifeact-GFP, the filamentous-actin-binding peptide Lifeact fused with the nuclear localization signal (NLS) and GFP, modulated the structure of nuclear actin filaments to be phalloidin-stainable in p53-depleted cells treated with the DNA-damaging agent, altering the chromatin structure and reducing the transcriptional activity. The level of phosphorylated H2AX (γH2AX), a marker of DNA damage, in these cells also reduced upon nLifeact-GFP expression, whilst details of the functional relationship between the formation of nLifeact-GFP-decorated nuclear actin filaments and DNA repair remained to be elucidated. Considering that the loss of p53 is associated with cancer progression, the results of this study raise a possibility that the artificial reinforcement of nuclear actin filaments by nLifeact-GFP may enhance the cytotoxic effect of DNA-damaging agents in aggressive cancer cells through a reduction in gene transcription.
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Kenji Usui, Makoto Ozaki, Kan Hirao, Tsubasa Kosaka, Natsumi Endo, Shuhei Yoshida, Shin-ichiro Yokota, Yonejiro Arimoto, Ryuji Osawa, Nobuhiro Nakanishi, Kin-ya Tomizaki, Tomohiro Umetani, Fumihiro Kayamori
Scientific Reports 13 12027 2023.7
Authorship：Last author, Corresponding author
Microwaves are used for diverse applications such as mobile phones, ovens, and therapy devices. However, there are few reports on the effects of microwaves on diseases other than cancer, and on physiological processes. Here, we focused on CaCO3 mineralization as a model of biomineralization and attempted to elucidate the effect of microwaves on CaCO3 mineralization using peptides. We conducted AFM, ζ potential, HPLC, ICP-AES, and relative permittivity measurements. Our findings show that microwaves alter the nanomorphology of the CaCO3 precipitate, from sphere-like particles to string-like structures. Furthermore, microwaves have little effect on the mineralization when the mineralization ability of a peptide is high, but a large effect when the precipitation ability is low. Our findings may be applicable to not only the treatment of teeth and bones but also the development of organic–inorganic nanobiomaterials. This methodology can be expanded to other molecular/atomic reactions under various microwave conditions to alter reaction activity parameters.
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Thixotropic β-Chitin Nanofiber Hydrogel with a Living Body-Responsive Self-Hardening Property and Its Application as a Sprayable Antiadhesion Barrier Reviewed International journal
Koji Nagahama, Kaho Tobiishi, Natsumi Ueda, Tatsuya Dode, Shahidul Islam Sobuj, Fumiya Yuasa, Kazuhiko Yamashita, Takahito Nishikata
ACS Applied Bio Materials 6 ( 7 ) 2636 - 2643 2023.7
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Effect of linearly polarized microwaves on nanomorphology of calcium carbonate mineralization using peptides Reviewed
Kenji Usui, Makoto Ozaki, Kan Hirao, Tsubasa Kosaka, Natsumi Endo, Shuhei Yoshida, Shin-ichiro Yokota, Yonejiro Arimoto, Ryuji Osawa, Nobuhiro Nakanishi, Kin-ya Tomizaki, Tomohiro Umetani, Fumihiro Kayamori
Scientific Reports 13 12027 2023.7
Microwaves are used for diverse applications such as mobile phones, ovens, and therapy devices. However, there are few reports on the effects of microwaves on diseases other than cancer, and on physiological processes. Here, we focused on CaCO3 mineralization as a model of biomineralization and attempted to elucidate the effect of microwaves on CaCO3 mineralization using peptides. We conducted AFM, ζ potential, HPLC, ICP-AES, and relative permittivity measurements. Our findings show that microwaves alter the nanomorphology of the CaCO3 precipitate, from sphere-like particles to string-like structures. Furthermore, microwaves have little effect on the mineralization when the mineralization ability of a peptide is high, but a large effect when the precipitation ability is low. Our findings may be applicable to not only the treatment of teeth and bones but also the development of organic–inorganic nanobiomaterials. This methodology can be expanded to other molecular/atomic reactions under various microwave conditions to alter reaction activity parameters.
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Inhibitory Effect of Adsorption of Streptococcus mutans onto Scallop-Derived Hydroxyapatite Reviewed
Momoko Usuda, Mariko Kametani, Masakazu Hamada, Yuto Suehiro, Saaya Matayoshi, Rena Okawa, Shuhei Naka, Michiyo Matsumoto-Nakano, Tatsuya Akitomo, Chieko Mitsuhata, Kazuya Koumoto, Keiko Kawauchi, Takahito Nishikata, Masatoshi Yagi, Toshiro Mizoguchi, Koki Fujikawa, Taizo Taniguchi, Kazuhiko Nakano and Ryota Nomura
Int J Mol Sci 24 11371 2023.7
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Inhibitory Effect of Adsorption of Streptococcus mutans onto Scallop-Derived Hydroxyapatite Reviewed
Usuda M, Kametani M, Hamada M, Suehiro Y, Matayoshi S, Okawa R, Naka S, Matsumoto-Nakano M, Akitomo T, Mitsuhata C, Koumoto K, Kawauchi K, Nishikata T, Yagi M, Mizoguchi T, Fujikawa K, Taniguchi T, Nakano K, Nomura R
International Journal of Molecular Science 24 11371 2023.7
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Inhibitory Effect of Adsorption of Streptococcus mutans onto Scallop-Derived Hydroxyapatite. Reviewed International journal
Momoko Usuda, Mariko Kametani, Masakazu Hamada, Yuto Suehiro, Saaya Matayoshi, Rena Okawa, Shuhei Naka, Michiyo Matsumoto-Nakano, Tatsuya Akitomo, Chieko Mitsuhata, Kazuya Koumoto, Keiko Kawauchi, Takahito Nishikata, Masatoshi Yagi, Toshiro Mizoguchi, Koki Fujikawa, Taizo Taniguchi, Kazuhiko Nakano, Ryota Nomura
International journal of molecular sciences 24 ( 14 ) 2023.7
Hydroxyapatite adsorbs various substances, but little is known about the effects on oral bacteria of adsorption onto hydroxyapatite derived from scallop shells. In the present study, we analyzed the effects of adsorption of Streptococcus mutans onto scallop-derived hydroxyapatite. When scallop-derived hydroxyapatite was mixed with S. mutans, a high proportion of the bacterial cells adsorbed onto the hydroxyapatite in a time-dependent manner. An RNA sequencing analysis of S. mutans adsorbed onto hydroxyapatite showed that the upregulation of genes resulted in abnormalities in pathways involved in glycogen and histidine metabolism and biosynthesis compared with cells in the absence of hydroxyapatite. S. mutans adsorbed onto hydroxyapatite was not killed, but the growth of the bacteria was inhibited. Electron microscopy showed morphological changes in S. mutans cells adsorbed onto hydroxyapatite. Our results suggest that hydroxyapatite derived from scallop shells showed a high adsorption ability for S. mutans. This hydroxyapatite also caused changes in gene expression related to the metabolic and biosynthetic processes, including the glycogen and histidine of S. mutans, which may result in a morphological change in the surface layer and the inhibition of the growth of the bacteria.
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Thixotropic β‑Chitin Nanofiber Hydrogel with a Living BodyResponsive Self-Hardening Property and Its Application as a Sprayable Antiadhesion Barrier Reviewed
Nagahama K, Tobiishi K, Ueda N, Dode T, Sobuj SI, Yuasa F, Yamashita K, Nishikata T
American Chemical Society Applied Bio Materials 2023 ( 6 ) 2636 - 2643 2023.7
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Co-generation of palladium nanoparticles and phosphate supported on metal-organic frameworks as hydrogenation catalysts Reviewed
Yohei Takashima, Seiko Tetsusashi, Mai Takano, Shintaro Tanaka, Yui Murakami, Takaaki Tsuruoka, Kensuke Akamatsu
Dalton Trans. 52 11158 - 11162 2023.7
Authorship：Lead author, Corresponding author
Other Link： https://pubs.rsc.org/en/content/articlelanding/2023/dt/d2dt04109j
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The iron chelator deferriferrichrysin induces paraptosis via extracellular signal-related kinase activation in cancer cells Invited Reviewed
Natsuki Kinoshita, Masaya Gessho, Takeru Torii, Yukako Ashida, Minori Akamatsu, Alvin Kunyao Guo, Sunmin Lee, Tatsuya Katsuno 3, Wataru Nakajima Yemima Budirahardja, Daisuke Miyoshi, Takehiko Todokoro, Hiroki Ishida, Takahito Nishikata, Keiko Kawauchi
Genes Cells 2023.6
DOI： 10.1111/gtc.13053
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Quantification of the concentration in a droplet formed by liquid–liquid phase separation of G-quadruplex-forming RNA Reviewed
Kohei Yokosawa, Mitsuki Tsuruta, Shinji Kajimoto, Naoki Sugimoto, Daisuke Miyoshi, Takakazu Nakabayashi
Chem. Phys. Lett. 826 140634 - 140634 2023.6
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The iron chelator deferriferrichrysin induces paraptosis via extracellular-signal-regulated kinase activation in cancer cells Reviewed International coauthorship
Kinoshita N, Gessho M, Torii T, Ashida Y, Akamatsu M, Guo AK, Lee S, Katsuno T, Nakajima W, Budirahardja Y, Miyoshi D, Todokoro T, Ishida H, Nishikata T, Kawauchi K
Genes to Cells 28 ( 9 ) 653 - 662 2023.6
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Simple and fast screening for structure-selective G-quadruplex ligands. Reviewed International journal
Yoshiki Hashimoto, Yoshiki Imagawa, Kaho Nagano, Ryuichi Maeda, Naho Nagahama, Takeru Torii, Natsuki Kinoshita, Nagisa Takamiya, Keiko Kawauchi, Hisae Tatesishi-Karimata, Naoki Sugimoto, Daisuke Miyoshi
Chemical communications (Cambridge, England) 59 ( 33 ) 4891 - 4894 2023.4
Joint Work
Structural selectivity of G-quadruplex ligands is essential for cellular applications since there is an excess of nucleic acids forming duplex structures compared to G-quadruplex structures in living cells. In this study, we developed new structure-selective G-quadruplex ligands utilizing a simple and fast screening system. The affinity, selectivity, enzymatic inhibitory activity and cytotoxicity of the structure-selective G-quadruplex ligands were demonstrated along with a structural selectivity-cytotoxicity relationship of G-quadruplex ligands.
DOI： 10.1039/d3cc00556a
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Not all 2',4'-bridged modifications stabilize DNA/RNA duplexes Reviewed
Tomoka Akita, Elisa Tomita-Sudo, Shin Itoh, Nae Sakimoto, Takeshi Masuda, Akifumi Nakamura, Yoshiyuki Onishi, Makoto Koizumi Junji Kawakami
Nucleosides, Nucleotides & Nucleic Acids 2023.3
Joint Work
2’,4’-Bridged modifications such as 2’-O,4’-C-methylene-bridged nucleotides (LNAs) and 2’-O,4’-C-ethylene-bridged nucleotides (ENAs) provide high binding affinity for duplex formation. Stabilization by the introduction of the bridged nucleic acids is considered to be due to pre-organization. In this study, we found that the introduction of 2’,4’-C-bridged 2’-deoxynucleotides (CRNs; Conformationally Restricted Nucleotides) into DNA/RNA duplexes leads to destabilization, as opposed to the previously accepted notion that 2’,4’-bridged modifications always lead to stabilization.
Other Link： https://www.tandfonline.com/doi/full/10.1080/15257770.2023.2232414
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Biomolecular Liquid–Liquid Phase Separation for Biotechnology Invited Reviewed
Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi and Daisuke Miyoshi
BioTech 12 ( 2 ) 26 2023.3
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Construction of a Method to Design Fibril-Forming Peptides Applied to Biomaterials from 20 Beta-Sheet Peptides by Statistical Analysis Reviewed
Kazuya Iwata, Taisei Terao, Akira Takekawa, Tomohiro Umetani, Kenji Usui
Peptide Science 2022 141 - 142 2023.3
Authorship：Last author, Corresponding author
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High-performance bulk Bi0.4Sb1.6Te3.0 thermoelectrics prepared from nanocrystal precursor synthesized via chemical precipitation Reviewed
Ryosuke Fujiwara, Yohei Takashima, Takaaki Tsuruoka, Muneyuki Naito, Junya Murai, Kensuke Akamatsu
Journal of Solid State Chemistry 319 123777 - 123777 2023.3
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Direct generation of polypyrrole-coated palladium nanoparticles inside a metal-organic framework for a semihydrogenation catalyst Reviewed
Yohei Takashima, Seiko Tetsusashi, Shintaro Tanaka, Takaaki Tsuruoka, Kensuke Akamatsu
RSC Adv. 13 7464 - 7467 2023.3
Authorship：Lead author
Other Link： https://pubs.rsc.org/en/content/articlelanding/2023/ra/d2ra08190c
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Simple and fast screening for structure-selective G-quadruplex ligands Reviewed International journal
Yoshiki Hashimoto, Yoshiki Imagawa, Kaho Nagano, Ryuichi Maeda, Naho Nagahama, Takeru Torii, Natsuki Kinoshita, Nagisa Takamiya, Keiko Kawauchi, Hisae Tatesishi-Karimata, Naoki Sugimoto and Daisuke Miyoshi
Chem. Commun. 59 ( 33 ) 4891 - 4894 2023.2
Authorship：Corresponding author
Structural selectivity of G-quadruplex ligands is essential for cellular applications since there is an excess of nucleic acids forming duplex structures compared to G-quadruplex structures in living cells. In this study, we developed new structure-selective G-quadruplex ligands utilizing a simple and fast screening system. The affinity, selectivity, enzymatic inhibitory activity and cytotoxicity of the structure-selective G-quadruplex ligands were demonstrated along with a structural selectivity-cytotoxicity relationship of G-quadruplex ligands.
DOI： 10.1039/D3CC00556A
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Morphological Evidence for Novel Roles of Microtubules in Macrophage Phagocytosis Reviewed
Seta Y, Kawakatsu K, Degawa S, Goto T, Nishikata T
International Journal of Molecular Science 24 1373 2023.1
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RNA 高次構造を標的とした光線力学療法の展望 Invited
取井猛流・木下菜月・橋本佳樹・杉本渉・川内敬子・三 好大輔
38 ( 13 ) 1154 - 1156 2023
Authorship：Last author, Corresponding author
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グアニン四重らせん構造による転移因子LINE-1の発現抑制名
月生 雅也, 李 先民, Yemima Suryani Budirahardja, 鶴田 充生, 橋本 佳樹, 高宮 渚, 木下 菜月, 建石 寿枝, 杉本 直己, 三好 大輔, 川内 敬子
日本女性科学者の会学術誌 23 48 - 48 2023
Publisher：(一社)日本女性科学者の会
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High-performance bulk Bi0.4Sb1.6Te3.0 thermoelectrics prepared from nanocrystal precursor synthesized via chemical precipitation
R. Fujiwara, Y.Takashima, T. Tsuruoka, M. Naito, J. Murai, K. Akamatsu
Journal of Solid State Chemistry 319 123777 2023
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“Direct generation of polypyrrole-coated palladium nanoparticles inside a metal-organic framework for a semihydrogenation catalyst
Y. Takashima, S. Tetsusashi, S. Tanaka, T. Tsuruoka, K. Akamatsu
RSC Adv 13 7464 - 7467 2023
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“Co-generation of palladium nanoparticles and phosphate supported on metal-organic frameworks as hydrogenation catalysts
Y. Takashima, S. Tetsusashi, M. Takano, S. Tanaka, Y. Murakami, T. Tsuruoka, K. Akamatsu
Dalton Trans 52 11158 - 11162 2023
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Yohei Takashima, Seiko Tetsusashi, Shintaro Tanaka, Takaaki Tsuruoka, Kensuke Akamatsu
RSC Advances 13 ( 11 ) 7464 - 7467 2023
Publisher：Royal Society of Chemistry (RSC)
Polypyrrole-coated palladium nanoparticles could be directly generated inside a metal–organic frameworks. The resulting MOF composites exhibited higher semihydrogenation capability than the analog composite without polypyrrole coating.
DOI： 10.1039/d2ra08190c
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Yohei Takashima, Seiko Tetsusashi, Mai Takano, Shintaro Tanaka, Yui Murakami, Takaaki Tsuruoka, Kensuke Akamatsu
Dalton Transactions 52 ( 32 ) 11158 - 11162 2023
Publisher：Royal Society of Chemistry (RSC)
In this study, we demonstrated the direct synthesis of sodium dihydrogen phosphate (PA) containing palladium nanoparticles (PdNPs) supported on a metal–organic framework (MOF) for hydrogenation catalysis.
DOI： 10.1039/d2dt04109j
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Evaluation of thermal stability of DNA oligonucleotide structures embedded in hydrogels Invited Reviewed International journal
D. Yamaguchi, M. Yoshida, S. Nakano
DNA 2 ( 4 ) 302 - 313 2022.12
Joint Work
Authorship：Last author, Corresponding author Publisher：MDPI
Understanding the self-assembly and hybridization properties of DNA oligonucleotides in confined spaces can help to improve their applications in biotechnology and nanotechnology. This study investigates the effects of spatial confinement in the pores of hydrogels on the thermal stability of DNA oligonucleotide structures. The preparation of oligonucleotides embedded in agarose gels was simple, whereas the preparation of oligonucleotides embedded in polyacrylamide gels was required to remove unpolymerized monomers. In the latter case, a method for rehydrating a washed dry gel with a buffer solution containing oligonucleotides was developed. Fluorescence measurements of oligonucleotides bearing fluorescent probes revealed no significant influence of the internal environment of the gel pores on the stability of DNA duplex, hairpin, and G-quadruplex structures. Moreover, the effects of poly(ethylene glycol) on the stability of DNA structures in the gels were similar to those in solutions. It is likely that the oligonucleotides are not strongly constrained in the gels and may be preferentially located in a water-rich environment in the gel matrix. The gel preparation was also applied to the assessment of the stability of DNA structures under the conditions of a reduced number of water molecules. The studies using hydrogels provide insights into the ability of self-assembly and hybridization of oligonucleotides in confined environments and under low-water-content conditions.
DOI： 10.3390/dna2040021
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Controlling liquid-liquid phase separation of G-quadruplex-forming RNAs in a sequence-specific manner. Reviewed International journal
Mitsuki Tsuruta, Takeru Torii, Kazuki Kohata, Keiko Kawauchi, Hisae Tateishi-Karimata, Naoki Sugimoto, Daisuke Miyoshi
Chemical communications (Cambridge, England) 58 ( 93 ) 12931 - 12934 2022.11
Joint Work
We constructed a minimum liquid-liquid phase separation model system to form liquid droplets using only G-quadruplex-forming oligonucleotides and R- and G-rich oligopeptides. We found that the G-quadruplex structure is an essential component for RNA to form droplets with the peptide. Based on this model system and our findings, droplet redissolution via structure transition from a G-quadruplex to a duplex was achieved in a sequence-specific manner.
DOI： 10.1039/d2cc04366a
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Covalent Stem Cell-Combining Injectable Materials with Enhanced Stemness and Controlled Differentiation In Vivo Reviewed
Natsumi Ueda, Shiho Sawada, Fumiya Yuasa, Karen Kato, Koji Nagahama
ACS Applied Materials & Interfaces 14 ( 47 ) 52618 - 52633 2022.11
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ポリエチレングリコールによるアトピー性皮膚炎予防の可能性
厳原美穂、甲元一也
Fragrance Journal 50 ( 11 ) 54 - 57 2022.11
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Controlling liquid–liquid phase separation of G-quadruplex-forming RNAs in a sequence-specific manner Reviewed International journal
Mitsuki Tsuruta, Takeru Torii, Kazuki Kohata, Keiko Kawauchi, Hisae Tateishi-Karimata, Naoki Sugimoto and Daisuke Miyoshi
Chem. Commun. 58 ( 93 ) 12931 - 12934 2022.11
We constructed a minimum liquid-liquid phase separation model system to form liquid droplets using only G-quadruplex-forming oligonucleotides and R- and G-rich oligopeptides. We found that the G-quadruplex structure is an essential component for RNA to form droplets with the peptide. Based on this model system and our findings, droplet redissolution via structure transition from a G-quadruplex to a duplex was achieved in a sequence-specific manner.
DOI： 10.1039/D2CC04366A
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Two-Dimensional Metal–Organic Framework-Based Cellular Scaffolds with High Protein Adsorption, Retention, and Replenishment Capabilities Reviewed
Tokitaka Katayama, Shintaro Tanaka, Takaaki Tsuruoka, and Koji Nagahama
ACS Applied Materials & Interfaces 14 ( 30 ) 34443 - 34454 2022.8
Authorship：Last author, Corresponding author
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Two-Dimensional Metal–Organic Framework-Based Cellular Scaffolds with High Protein Adsorption, Retention, and Replenishment Capabilities Reviewed
Tokitaka Katayama, Shintaro Tanaka, Takaaki Tsuruoka, Koji Nagahama
ACS Applied Materials & Interfaces 2022.8
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Evaluation of weak interactions of proteins and organic cations with DNA duplex structures Reviewed International journal
R. Morimoto, M. Horita, D. Yamaguchi, H. Nakai, and S. Nakano
Biophys. J. 121 ( 15 ) 2873 - 2881 2022.8
Joint Work
Authorship：Last author, Corresponding author Publisher：Cell Press
Molecular interactions and reactions in living cells occur with high background concentrations of organic compounds including proteins. Uncharged water-soluble polymers are commonly used cosolutes in studies on molecular crowding, and most studies argue about the effects of intracellular crowding based on results obtained using polymer cosolutes. Further investigations using protein crowders and organic cations are important in understanding the effects of cellular environments on nucleic acids with negatively charged surfaces. We assessed the effects of using model globular proteins, serum proteins, histone proteins, structurally flexible polypeptides, di- and polyamines, and uncharged polymers. Thermal stability analysis of DNA oligonucleotide structures revealed that unlike conventional polymer cosolutes, basic globular proteins (lysozyme and cytochrome c) at high concentrations stabilized long internal and bulge loop structures but not fully matched duplexes. The selective stabilization of long loop structures suggests preferential binding to unpaired nucleotides in loops through weak electrostatic interactions. Furthermore, the ability of the proteins to stabilize the loop structures was enhanced under macromolecular crowding conditions. Remarkably, the effects of basic proteins on the stability of fully matched duplexes were dissimilar to those of basic amino-acid-rich polypeptides and polyamines. This study provides new insights into the interaction of nucleic acid structures with organic cations.
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細胞内環境で安定化される核酸非構造を狙った分子標的薬の開発 Invited
川内敬子・橋本佳樹・杉本 渉・三好大輔
バイオマテリアル = Journal of Japanese Society for Biomaterials : 生体材料 40 ( 3 ) 206 - 211 2022.7
Authorship：Corresponding author
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核酸の化学修飾と構造や相分離能の相関 Invited
鶴田 充生・取井 猛流・杉本 渉・川内 敬子・三好 大輔
月刊「細胞」（ニューサイエンス社） 53 ( 14 ) 910 - 913 2022.6
Authorship：Last author, Corresponding author
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Exportin-inspired artificial cell nuclear-exporting nanosystems Reviewed
Mitsuo Inui , Yuta Hamada , Nana Sejima , Natsumi Ueda , Tokitaka Katayama , Kimika Ono, Koji Nagahama
Nanoscale Advances 4 2637 - 2641 2022.5
Authorship：Last author, Corresponding author
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Significant structural change in human c-Myc promoter G-quadruplex Reviewed International coauthorship
Nikita Kundu, Taniya Sharma, Sarvpreet Kaur, Mamta Singh, Vinit Kumar, Uttam Sharma, Aklank Jain, Jadala Shankaraswamy, Daisuke Miyoshi, Sarika Saxena
RSC Adv. 12 7594 - 7604 2022.4
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Kimika Ono, Manami Sumiya, Naohiro Yoshinobu, Tatsuya Dode, Tokitaka Katayama, Natsumi Ueda, Koji Nagahama
ACS Applied Bio Materials 5 ( 2 ) 471 - 482 2022.2
Authorship：Last author, Corresponding author
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Toshiyuki Goto, Shuhei Torii, Aoi Kondo, Kazumasa Kanda, Junji Kawakami, Yosky Kataoka, Takahito Nishikata
Journal of Developmental Biology 10 ( 1 ) 10 - 10 2022.2
Publisher：MDPI AG
In many animal species, the body axis is determined by the relocalization of maternal determinants, organelles, or unique cell populations in a cytoskeleton-dependent manner. In the ascidian first cell cycle, the myoplasm, including mitochondria, endoplasmic reticulum (ER), and maternal mRNAs, move to the future posterior side concomitantly (called ooplasmic segregation or cytoplasmic and cortical reorganization). This translocation consists of first and second phases depending on the actin and microtubule, respectively. However, the transition from first to second phase, that is, translocation of myoplasmic components from microfilaments to microtubules, has been poorly investigated. In this study, we analyzed the relationship between these cytoskeletons and myoplasmic components during the first cell cycle and their role in morphogenesis by inhibitor experiments. Owing to our improved visualization techniques, there was unexpected F-actin accumulation at the vegetal pole during this transition period. When this F-actin was depolymerized, the microtubule structure was strongly affected, the myoplasmic components, including maternal mRNA, were mislocalized, and the anteroposterior axis formation was disordered. These results suggested the importance of F-actin during the first cell cycle and the existence of interactions between microfilaments and microtubules, implying the enigmatic mechanism of ooplasmic segregation. Solving this mystery leads us to an improved understanding of ascidian early development.
DOI： 10.3390/jdb10010010
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Dynamic Changes in the Aassociation between Maternal mRNAs and Endoplasmic Reticulum during Ascidian Early Embryogenesis. Reviewed
Goto T, Torii S, Kondo A, Yagi H, Suekane M, Kataoka Y, Nishikata T
Development Genes and Evolution 232 ( 1 ) 1 - 14 2022.2
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Actin Filament in the First Cell Cycle Contributes to the Determination of the Anteroposterior Axis in Ascidian Development. Reviewed
Goto T, Torii S, Kondo A, Kanda K, Kataoka Y, Nishikata T
Journal of Developmental Biology 10 ( 1 ) 10 - 23 2022.2
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Intramolecular G-quadruplex-hairpin loop structure competition of a GC-rich exon region in the TMPRSS2 gene Reviewed
Chem. Commun. 58 48 - 51 2022.1
Authorship：Corresponding author
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De novo design of a nanopore for single-molecule detection that incorporates a β-hairpin peptide. Reviewed International coauthorship International journal
Keisuke Shimizu, Batsaikhan Mijiddorj, Masataka Usami, Ikuro Mizoguchi, Shuhei Yoshida, Shiori Akayama, Yoshio Hamada, Akifumi Ohyama, Kenji Usui, Izuru Kawamura, Ryuji Kawano
Nature nanotechnology 17 67 - 75 2022.1
The amino-acid sequence of a protein encodes information on its three-dimensional structure and specific functionality. De novo design has emerged as a method to manipulate the primary structure for the development of artificial proteins and peptides with desired functionality. This paper describes the de novo design of a pore-forming peptide, named SV28, that has a β-hairpin structure and assembles to form a stable nanopore in a bilayer lipid membrane. This large synthetic nanopore is an entirely artificial device for practical applications. The peptide forms multidispersely sized nanopore structures ranging from 1.7 to 6.3 nm in diameter and can detect DNAs. To form a monodispersely sized nanopore, we redesigned the SV28 by introducing a glycine-kink mutation. The resulting redesigned peptide forms a monodisperse pore with a diameter of 1.7 nm leading to detection of a single polypeptide chain. Such de novo design of a β-hairpin peptide has the potential to create artificial nanopores, which can be size adjusted to a target molecule.
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Chemical synthesis of single nanometer-sized Bi2−xSbxTe3.0 nanocrystals via direct precipitation process Reviewed
Ryosuke Fujiwara, Yohei Takashima, Takaaki Tsuruoka, Muneyuki Naito, Junya Murai, Kensuke Akamatsu
Results in Chemistry 4 100485 - 100485 2022.1
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Wataru Sugimoto, Natsuki Kinoshita, Minori Nakata, Tatsuya Ohyama, Hisae Tateishi-Karimata, Takahito Nishikata, Naoki Sugimoto, Daisuke Miyoshi, Keiko Kawauchi
Chemical Communications 58 ( 1 ) 48 - 51 2022
Publisher：Royal Society of Chemistry (RSC)
We identified cytosine-rich regions adjacent to guanine-rich regions in the TMPRSS2 gene, which showed structural competition between a G-quadruplex and a hairpin loop. Furthermore, this competition significantly affected transcription efficiency.
DOI： 10.1039/d1cc05523b
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核酸の四重らせん構造を狙った治療薬の展望 Invited
37 ( 4 ) 52 - 55 2022
Authorship：Corresponding author
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rDNAおよびrRNAが形成する四重らせん構造による核小体機能の制御 Invited
川内敬子、取井猛流、月生雅也、木下菜月、三好大輔
月刊細胞 54 ( 12 ) 36 - 39 2022
Authorship：Corresponding author
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Rational and site-selective formation of coordination polymer consisiting of d10 coinage metal ions with thiolate ligands using metal ion-doped polymer substrate
T. Tsuruoka, Y. Miyashita, R. Yoshino, M. Fukuoka, S. Hirao, Y. Takashima, A. Demessence, K. Akamatsu
RSC Adv 12 3716 - 3720 2022
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Chemical synthesis of single nanometer-sized Bi2-xSbxTe3.0 nanocrystals via direct precipitation process
R. Fujiwara, Y.Takashima, T. Tsuruoka, M. Naito, J. Murai, K. Akamatsu
Chemistry 4 100485 2022
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ポリイミド基板上への選択的金属有機構造体の形成技術
福岡美海、江口慧一、髙嶋洋平、赤松謙祐、鶴岡孝章
ポリイミドの高機能設計と応用技術 6 ( 6 ) 487 - 495 2022
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Cooperative catalysis in a metal–organic framework <i>via</i> post-synthetic immobilisation Reviewed
Yohei Takashima, Yuya Suwaki, Takaaki Tsuruoka, Kensuke Akamatsu
Dalton Transactions 51 ( 24 ) 9229 - 9232 2022
Publisher：Royal Society of Chemistry (RSC)
A Co-salen complex and a MOF were successfully hybridized by post-synthetic immobilization for a cooperative catalytic system.
DOI： 10.1039/d2dt01544g
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Rational and site-selective formation of coordination polymers consisting of d<sup>10</sup> coinage metal ions with thiolate ligands using a metal ion-doped polymer substrate Reviewed International coauthorship
Takaaki Tsuruoka, Yuri Miyashita, Ryuki Yoshino, Myu Fukuoka, Shoya Hirao, Yohei Takashima, Aude Demessence, Kensuke Akamatsu
RSC Advances 12 ( 6 ) 3716 - 3720 2022
Authorship：Lead author, Corresponding author Publisher：Royal Society of Chemistry (RSC)
We report an interfacial approach for fabricating coordination polymer (CP) crystal films and patterns on a polymer surface using a metal ion-doped polymer substrate.
DOI： 10.1039/d2ra00269h
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ポリイミド基板上への選択的金属有機構造体の形成技術
福岡美海、江口慧一、髙嶋洋平、赤松謙祐、鶴岡孝章
ポリイミドの高機能設計と応用技術 6 ( 6 ) 487 - 495 2022
Authorship：Corresponding author
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Rational and site-selective formation of coordination polymer consisiting of d10 coinage metal ions with thiolate ligands using metal ion-doped polymer substrate Reviewed International coauthorship
T. Tsuruoka, Y. Miyashita, R. Yoshino, M. Fukuoka, S. Hirao, Y. Takashima, A. Demessence, K. Akamatsu
RSC Adv 12 3716 - 3720 2022
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Anti-Malignant Effect of Tensile Loading to Adherens Junctions in Cutaneous Squamous Cell Carcinoma Cells Reviewed International journal
Dobrokhotov O, Sunagawa M, Torii T, Mii S, Kawauch K, Enomoto A, Sokabe M, Hirata H.
Frontiers in Cell and Developmental Biology 11 2021.11
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Injectable Biocatalytic Nanocomposite Hydrogel Factories for Focal Enzyme-Prodrug Cancer Therapy Reviewed
Kimika Ono, Hiroyuki Hashimoto, Tokitaka Katayama, Natsumi Ueda, Koji Nagahama
Biomacromolecules 22 ( 10 ) 4217 - 4227 2021.10
Authorship：Last author, Corresponding author
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Supercapacitor electrode with high charge density based on boron-doped porous carbon derived from covalent organic frameworks Reviewed
Shigeyuki Umezawa, Takashi Douura, Koji Yoshikawa, Yohei Takashima, Mika Yoneda, Kazuma Gotoh, Vlad Stolojan, S. Ravi P. Silva, Yasuhiko Hayashi, Daisuke Tanaka
Carbon 184 418 - 425 2021.10
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Koji Nagahama, Seika Aoyama, Natsumi Ueda, Yuka Kimura, Tokitaka Katayama, Kimika Ono
ACS Macro Letters 10 ( 8 ) 1073 - 1079 2021.8
Authorship：Lead author, Corresponding author
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Involvement of Annexin A2 in Serum-MAF Dependent Phagocytic Activation of Macrophages. Reviewed
Kawakatsu K, Seta Y, Maemura M, Nishikata T.
Anticancer Research 41 ( 8 ) 4089 - 4092 2021.8
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ウォッシャーディスインフェクターのすすぎ排水中に含まれる洗浄剤残留量の測定によるすすぎ性能評価 Reviewed
三軒隼人、藤田敏、川田原瑠勇、武川公、臼井健二、原田陽滋
医療機器学 91 315 - 332 2021.8
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Enhancement of the Catalytic Activity of Hammerhead Ribozymes by Organic Cations. Reviewed International journal
Shu-Ichi Nakano, Hirofumi Yamashita, Naoki Sugimoto
ChemBioChem 22 ( 17 ) 2721 - 2728 2021.7
Joint Work
Authorship：Lead author, Corresponding author
Catalytic turnover is important for the application of ribozymes to biotechnology. However, the turnover is often impaired because of the intrinsic high stability of base pairs with cleaved RNA products. Here, organic cations were used as additives to improve the catalytic performance of hammerhead ribozyme constructs that exhibit different kinetic behaviors. Kinetic analysis of substrate cleavage demonstrated that bulky cations, specifically tetra-substituted ammonium ions containing pentyl groups or a benzyl group, have the ability to greatly increase the turnover rate of the ribozymes. Thermal stability analysis of RNA structures revealed that the bulky cations promote the dissociation of cleaved products and refolding of incorrectly folded structures with small disruption of the catalytic structure. The use of bulky cations is a convenient method for enhancing the catalytic activity of hammerhead ribozymes, and the approach may be useful for advancing ribozyme technologies.
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Zayed, M., Iohara, K., Watanabe, H., Ishikawa, M., Tominaga, M. and Nakashima, M.
Stem Cell Res Ther. 12 ( 302 ) 2021.5
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Detection of Intracellular Reactive Oxidative Species Using the Fluorescent Probe Hydroxyphenyl Fluorescein Invited Reviewed
Wataru Sugimoto, Daisuke Miyoshi, Keiko Kawauchi
Methods in Molecular Biology 2274 207 - 215 2021.5
Publisher：Humana Press Inc.
Various fluorescent probes for the detection of intracellular reactive oxidative species (ROS) have been developed because ROS levels are closely associated with cellular states. Here, we describe a method for detection of intracellular ROS in living cells using the fluorescent probe, hydroxyphenyl fluorescein (HPF), which detects hydroxyl radicals and peroxynitrite. NIH3T3 cells and p53 knockout (p53−/−) mouse embryonic fibroblasts (MEFs) were transformed by expressing oncogenic RAS using a retrovirus system. The cells were treated with HPF at 37 °C for 30 min, and subsequently, images were acquired using a confocal fluorescence microscope at an excitation wavelength of 488 nm after washing with PBS.
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Molecularly targeted photodynamic therapy for cancer mRNAs Invited Reviewed
杉本渉, 橋本佳樹, 川内敬子, 三好大輔
Bio Clinica 36 ( 5 ) 65 - 67 2021.5
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Controlling interfacial ion-transport kinetics using polyelectrolyte membranes for additive- and effluent-free, high-performance electrodeposition Reviewed International journal
K. Akamatsu, S. Nakano, K. Kimura, Y. Takashima, T. Tsuruoka, H. Nawafune, Y. Sato, J. Murai, and H. Yanagimoto
ACS Appl. Mater. Interfaces 13 ( 11 ) 13896 - 13906 2021.3
Joint Work
Publisher：ACS Publications
The development of high-performance, environmentally friendly electrodeposition processes is critical for emerging coating technologies because current technologies use highly complex baths containing metal salts, supporting electrolytes, and various kinds of organic additives, which are problematic from both environmental and cost perspectives. Here, we show that a 200 μm-thin polyelectrolyte membrane sandwiched between electrodes effectively concentrates metal ions through interfacial penetration, which increases the conductance between the electrodes to 0.30 S and realizes solid-state electrodeposition that produces no mist, sludge, or even waste effluent. Both, experimental results and theoretical calculations, reveal that electrodeposition is controlled by ion penetration at the solution/polyelectrolyte interface, providing an intrinsically different ion-transport mechanism to that of conventional diffusion-controlled electrodeposition. The setup, which includes 0.50 mol L–1 copper sulfate and no additives, delivers a maximum current density of 300 mA cm–2, which is nearly fivefold higher than that of a current commercial plating bath containing organic additives.
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核小体ストレスにより形成するrDNA G-quadruplex集合体とその機能
杉本 渉, 桑本 慎, 建石 寿枝, 岩根 敦子, 高橋 宏隆, 三好 大輔, 川内 敬子
日本薬学会年会要旨集 141年会 29V05 - am10S 2021.3
Publisher：(公社)日本薬学会
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Covalent cell-loading injectable hydrogel scaffold significantly promotes tissue regeneration in vivo compared with a conventional physical cell-loading hydrogel scaffold Reviewed
Yuka Kimura, Seika Aoyama, Natsumi Ueda, Tokitaka Katayama, Kimika Ono, Koji Nagahama
Advanced Biology 5 2000106 2021.2
Joint Work
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Hydrostatic Pressure-Regulated Cellular Calcium Responses. Reviewed International journal
Minami Fukuchi, Kotaro Oyama, Hiroaki Mizuno, Akihisa Miyagawa, Kazuya Koumoto, Gaku Fukuhara
Langmuir : the ACS journal of surfaces and colloids 37 ( 2 ) 820 - 826 2021.1
Joint Work
Hydrostatic pressure control has attracted much attention and presents a still challenging objective from mechanobiological viewpoints. Herein, we reveal the calcium entry processes in HeLa cells by means of hydrostatic pressure spectroscopy. The steady-state fluorescence spectral data comprehensively elucidated the factors controlling the outcomes of the hydrostatic pressure-stimulated calcium entry behavior. The present work leads to a new perspective on ion regulations in living cells and an attractive alternative to conventional mechanostimuli.
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Combined Effects of Methylated Cytosine and Molecular Crowding on the Thermodynamic Stability of DNA Duplexes Invited Reviewed
Combined Effects of Methylated Cytosine, Molecular Crowding, on, the Thermodynamic Stability of, DNA Duplexes
Int. J. Mol. Sci. 22 ( 2 ) 947 2021.1
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Exploration of structural transition phenomenon in flexible metal-organic framework formed on polymer substrate
S. Hirao, R. Hamagami, T. Ohhashi, K. Eguchi, N. Kubo, Y. Takashima, K. Akamatsu, T. Tsuruoka
CrystEngComm 23 8498 - 8505 2021
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Precisely Controlled Reproducible Synthesis of Palladium Nanoparticles inside Metal-Organic Frameworks with H2 Gas as Reductant: Effects of Framework Crystallinity and H2 Gas
Y. Takashima, Y. Sato, N.Kubo, T. Tsuruoka, K. Akamatsu
Chem. Lett 50 244 - 247 2021
Joint Work
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Controlling Interfacial Ion-Transport Kinetics Using Polyelectrolyte Membranes for Additive- and Effluent-free, High-Performance Electrodeposition Reviewed
Kensuke Akamatsu, Shu-ichi Nakano, Koshi Kimura, Yohei Takashima, Takaaki Tsuruoka, Hidemi Nawafune, Yuki Sato, Junya Murai, Hiroshi Yanagimoto
ACS Applied Materials&Interfaces 2021
Joint Work
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Ozaki, M., Yoshida, S., Tsuruoka, T., Usui, K.
Chemical Communications 57 2021
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Ozaki, M., Imai, T., Tsuruoka, T., Sakashita, S., Tomizaki, K.-Y., Usui, K.
Communications Chemistry 4 2021
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Shoya Hirao, Ruho Hamagami, Takashi Ohhashi, Keiichi Eguchi, Neo Kubo, Yohei Takashima, Kensuke Akamatsu, Takaaki Tsuruoka
CrystEngComm 23 ( 48 ) 8498 - 8505 2021
Authorship：Last author, Corresponding author Publisher：Royal Society of Chemistry (RSC)
We investigate the structural transition of directly formed flexible MOF crystals on a polymer substrate.
DOI： 10.1039/d1ce01383a
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Precisely Controlled Reproducible Synthesis of Palladium Nanoparticles inside Metal-Organic Frameworks with H2 Gas as Reductant: Effects of Framework Crystallinity and H2 Gas Reviewed
Y. Takashima, Y. Sato, N.Kubo, T. Tsuruoka, K. Akamatsu
Chem. Lett 50 244 - 247 2021
Joint Work
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Intracellular mineralization of gold nanoparticles using gold ion-binding peptides with cell-penetrating ability Reviewed
Makoto Ozaki, Shuhei Yoshida, Takaaki Tsuruoka, Kenji Usui
Chemical Communications 2021
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Photosensitizers Based on G-Quadruplex Ligand for Cancer Photodynamic Therapy Invited International journal
Keiko Kawauchi, Ryoto Urano, Natsuki Kinoshita, Shin Kuwamoto, Takeru Torii, Yoshiki Hashimoto, Shinya Taniguchi, Mitsuki Tsuruta, Daisuke Miyoshi
Genes 11 ( 11 ) 1340 2020.11
Joint Work
G-quadruplex (G4) is the non-canonical secondary structure of DNA and RNA formed by guanine-rich sequences. G4-forming sequences are abundantly located in telomeric regions and in the promoter and untranslated regions (UTR) of cancer-related genes, such as RAS and MYC. Extensive research has suggested that G4 is a potential molecular target for cancer therapy. Here, we reviewed G4 ligands as photosensitizers for cancer photodynamic therapy (PDT), which is a minimally invasive therapeutic approach. The photosensitizers, such as porphyrins, were found to be highly toxic against cancer cells via the generation of reactive oxidative species (ROS) upon photo-irradiation. Several porphyrin derivatives and analogs, such as phthalocyanines, which can generate ROS upon photo-irradiation, have been reported to act as G4 ligands. Therefore, they have been implicated as promising photosensitizers that can selectively break down cancer-related DNA and RNA forming G4. In this review, we majorly focused on the potential application of G4 ligands as photosensitizers, which would provide a novel strategy for PDT, especially molecularly targeted PDT (mtPDT).
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Precisely Controlled Reproducible Synthesis of Palladium Nanoparticles inside Metal-Organic Frameworks with H2 Gas as Reductant: Effects of Framework Crystallinity and H2 Gas Pressure Reviewed
Yohei Takashima, Yasushi Sato, Neo Kubo, Takaaki Tsuruoka, Kensuke Akamatsu
Chem. Lett. 50 ( 2 ) 244 - 247 2020.11
Authorship：Lead author, Corresponding author
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TakahiroYamauchi, Takafumi Kubodera, Daisuke Miyoshi, Naoki Sugimoto, Shuji Hirohata
Journal of Bioscience and Bioengineering 2020.10
Joint Work
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Mechanism toward Turn-on of Polysaccharide-Porphyrin Complexes for Fluorescence Probes and Photosensitizers in Photodynamic Therapy in Living Cells Reviewed International journal
Shodai Hino, Rikushi Funada, Kouta Sugikawa, Riku Kawasaki, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Atsushi Ikeda
CHEMMEDCHEM 15 ( 1 ) 1 - 12 2020.10
Joint Work
Publisher：WILEY-V C H VERLAG GMBH
beta-(1,3-1,6)-D-Glucan, lambda-carrageenan, tamarind gum, and pullulan can dissolve various porphyrin derivatives via the formation of complexes in water using a high-speed vibration milling method. The aqueous solutions of the resulting complexes exhibit long-term stability. Despite the adverse effects of the self-quenching process, notable fluorescence and improved photodynamic activity of the polysaccharide-complexed porphyrin derivatives were observed in the presence of liposomes, micelles, cyclodextrins, and HeLa cells. It was noted that the type of porphyrins was more important than the type of polysaccharides present in the complex. Porphyrin self-aggregates were monodispersed in the lipid membranes of the liposomes and lysosomes. The polysaccharide-complexed porphyrin derivatives showed increased photodynamic activity toward HeLa cells under photoirradiation between 610 and 740 nm.
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Novel Strategy of Photodynamic Therapy Targeting RAS mRNA with G-Quadruplex Ligands for Cancer Treatment Invited Reviewed International journal
Takeru Torii, Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi
Journal of Data Mining in Genomics & Proteomics 11 ( 2 ) 2020.8
Joint Work
Authorship：Corresponding author
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Dermal fibroblasts internalize phosphatidylserine-exposed secretory melanosome clusters and apoptotic melanocytes. Reviewed
21 ( 16 ) 5789 2020.8
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Modification of the Release Rate of Curcumin from the Cavity of Renatured beta-1,3-1,6-Glucan Nanoparticles by Changing the Molecular Weight of the Constituent Polysaccharide Chains Reviewed
Yuki Nonoguchi, Ayumu Kodama, Rika Unesaki, Nanako Doi, Toshio Suzuki, Kazuya Koumoto
CHEMISTRY LETTERS 49 ( 10 ) 1150 - 1153 2020.7
Joint Work
Publisher：CHEMICAL SOC JAPAN
We prepared renatured beta-1,3-1,6-glucan nanoparticles from molecular-weight-fractionated beta-1,3-1,6-glucans to analyze the molecular effects on the inclusion and release of the guest molecule curcumin. Glucan molecular weight strongly affects the amount of curcumin included in the complexes and its release rate. We found that the slow release rate of the incorporated curcumin is attributed not only to the stabilized stacking structure but also the slow migration between the internal and surface cavities characteristic of polymeric materials.
DOI： 10.1246/cl.200366
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Photodynamic therapy targeting RAS mRNA
Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi
Medical Science Digest 46 ( 9 ) 26 - 27 2020.7
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Competing Roles of Two Kinds of Ligand during Nonclassical Crystallization of Pillared-Layer Metal-Organic Frameworks Elucidated Using Microfluidic Systems
Yoko Tanaka, Yu Kitamura, Ryuji Kawano, Kan Shoji, Moe Hiratani, Tetsuo Honma, Hikaru Takaya, Hirofumi Yoshikawa, Takaaki Tsuruoka, Daisuke Tanaka
Chemistry - A European Journal 26 ( 41 ) 8889 - 8896 2020.7
© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim To diversify metal-organic frameworks (MOFs), multi-component MOFs constructed from more than two kinds of bridging ligand have been actively investigated due to the high degree of design freedom afforded by the combination of multiple ligands. Predicting the synthesis conditions for such MOFs requires an understanding of the crystallization mechanism, which has so far remained elusive. In this context, microflow systems are efficient tools for capturing non-equilibrium states as they facilitate precise and efficient mixing with reaction times that correspond to the distance from the mixing point, thus enabling reliable control of non-equilibrium crystallization processes. Herein, we prepared coordination polymers with pillared-layer structures and observed the intermediates in the syntheses with an in-situ measurement system that combines microflow reaction with UV/Vis and X-ray absorption fine-structure spectroscopies, thereby enabling their rapid nucleation to be monitored. Based on the results, a three-step nonclassical nucleation mechanism involving two kinds of intermediate is proposed.
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Novel Strategy of Photodynamic Therapy Targeting RAS mRNA with G-Quadruplex Ligands for Cancer Treatment Invited Reviewed
Takeru Torii, Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi
Journal of Data Mining in Genomics & Proteomics 2020.6
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Miho Takagi-Sato, Koji Morita, Yoshiyuki Onishi, Yuuka Watahiki, Taku Ishigaki, Tomoka Akita, Erisa Tomita, Junji Kawakami, Makoto Koizumi
Nucleosides, Nucleotides & Nucleic Acids 39 ( 6 ) 838 - 852 2020.6
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Comparison of macrophage activation using γ-globulin and serum-derived macrophage activating factor. Reviewed
Akamatsu M, Mashiba R, Kawakatsu K, Ishikawa M, Nishikata T.
Anticancer Research 40 ( 8 ) 4707 - 4710 2020.6
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RNA Phase Separation-Mediated Direction of Molecular Trafficking Under Conditions of Molecular Crowding Invited Reviewed
Kazuki Kohata, Daisuke Miyoshi
Biophys. Rev. 12 669 - 676 2020.5
Joint Work
Living cells are highly crowded with large and small biomolecules. The total concentration of biomolecules can reach 400 mg/ml, and 40% of the cell volume is occupied by biomolecules. Droplet formation in cells via liquid-liquid phase separation may play a role in controlling biochemical reactions in this complex molecular environment. Liquid-liquid phase separation generally involves nucleic acids and proteins as anionic and cationic components, respectively. Significant characteristics of droplets, which make them different from protein aggregation or fibril formation, are reversibility of formation and responsiveness to the molecular environment. In this review, we quantitatively describe the molecular environment inside cells and droplets that participate in controlling central dogma reactions. Finally, we discuss the importance of droplets under conditions of molecular crowding within living cells.
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Miyazaki, H., Hamada, Y., Takaishi, H., Minamino, Y., Ikeda, H., Mekata, H., Takaishi, M., Yamashita, K., Usui, K.
Analyst 145 3211 - 3216 2020.5
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Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA Reviewed
H. Tateishi-Karimata, D. Banerjee, T. Ohyama, S. Matsumoto, D. Miyoshi, S. Nakano, and N. Sugimoto
Biochemical and Biophysical Research Communications 525 ( 1 ) 177 - 183 2020.4
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Coordination-Driven Self-Assembly on Polymer Surfaces for Efficient Synthesis of [Au(SPh)]<inf>n</inf> Coordination Polymer-Based Films
Takaaki Tsuruoka, Takashi Ohhashi, Jumpei Watanabe, Rikuto Yamada, Shoya Hirao, Yohei Takashima, Aude Demessence, Shefali Vaidya, Oleksandra Veselska, Alexandra Fateeva, Kensuke Akamatsu
Crystal Growth and Design 20 ( 3 ) 1961 - 1968 2020.3
Joint Work
Publisher：American Chemical Society ({ACS})
We describe a simple bottom-up strategy to generate [Au(SPh)] (SPh: thiophenolate) coordination polymer (CP) based thin films by employing an interfacial self-assembly on a nylon substrate. This method utilizes Au ion-doped nylon film as a precursor of [Au(SPh)] CP and a support substrate to control the kinetics of the reduction of the Au ion from Au(III) to Au(I) and the complexation of Au(I) ions as well as SPh on the surface of the nylon film. The obtained CPs exhibit a fibril morphology with high crystallinity, which leads to a luminescent property. Further, we systematically study the formation mechanism of [Au(SPh)] CPs on the substrates. Amorphous CPs formed at an early stage of reaction are transformed to crystalline CPs based on the rearrangement of components during the reaction. The findings described herein offer novel and interesting openings for the synthesis of Au ion-based CPs that demonstrate desired functions for a wide range of applications. n n n
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Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA. Reviewed International journal
Hisae Tateishi-Karimata, Dipanwita Banerjee, Tatsuya Ohyama, Saki Matsumoto, Daisuke Miyoshi, Shu-Ich Nakano, Naoki Sugimoto
Biochemical and biophysical research communications 2020.2
Telomeric G-quadruplex topology has the ability to regulate telomerase activity, which counteracts the shortening of telomere with successive cell divisions, thereby causing genomic longevity. However, the detailed mechanism of G-quadruplexes topologies formed by telomeric sequences requires further investigation. In this study, we quantitatively investigated the effect of cosolutes, particularly the varying number of hydroxyl groups, on the structural transition between hybrid type and parallel G-quadruplexes formed by telomeric DNA sequences. Cosolutes with one or no hydroxyl groups in the vicinal position more efficiently induced the transition to parallel G-quadruplex from hybrid G-quadruplex than those with more hydroxyl groups. We also examined the effect of cosolute structures on the hydration of G-quadruplex formation; the results indicated that cosolutes with fewer hydroxyl groups lead to the release of greater amount of water during G-quadruplex formation. Molecular dynamics results showed that the parallel G-quadruplex was more dehydrated than the hybrid type G-quadruplex. Generally, a dehydrated structure is favored under crowding condition. Thus, depending on the surrounding cosolutes, the G-quadruplex topology can be controlled by the G-quadruplex hydration state.
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Solubilization of poorly water-soluble bioactive molecules in neutral aqueous media by complexation with renatured beta-1,3-1,6-glucan nanoparticles Reviewed International journal
Ayumu Kodama, Akifumi Nakagawa, Yuki Nonoguchi, Haruka Sakurai, Chieko Yano, Toshio Suzuki, Kazuya Koumoto
BIOPOLYMERS 111 ( 4 ) e23349 2020.2
Joint Work
Publisher：WILEY
The design of scaffolds for solubilizing/dispersing poorly water-soluble bioactive molecules in neutral aqueous media is a major challenge of functional food, pharmaceuticals, and cosmetics development, as highlighted by the plethora of corresponding solubilization/dispersion strategies. Herein, renatured beta-1,3-1,6-glucan (r-glucan) nanoparticles prepared by neutralization of alkali-denatured beta-1,3-1,6-glucan and subsequent centrifugation are used as a host to disperse water-insoluble bioactive molecules (curcumin, all-trans-retinoic acid, and rebamipide) by simple mixing of host and guest solutions. Curcumin in the r-glucan cavity is found to be stacked in the form of J-aggregates and twisted along the helix, and is demonstrated to be retained for significantly longer than curcumin in the corresponding gamma-cyclodextrin (gamma-CD) complex. Specifically, curcumin incorporated in gamma-CD is released within 5.5 hours, whereas that in the r-glucan complex is released very slowly, with 12% of curcumin in the latter complex retained after 31-day incubation at 37 degrees C. Thus, inclusion protocol simplicity and slow release ability make r-glucan nanoparticles a potential carrier scaffold for various applications.
DOI： 10.1002/bip.23349
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MMP24 as a Target of YAP is a Potential Prognostic Factor in Cancer Patients Reviewed International journal
Wataru Sugimoto, Katsuhiko Ito, Hiroaki Hirata, Yoshinori Abe, Takeru Torii, Yasumasa Mitsui, Yemima Budirahardja, Nobuyuki Tanaka, Keiko Kawauchi
Bioengineering 7 ( 18 ) 1 - 12 2020.2
Joint Work
Authorship：Last author, Corresponding author
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Therapeutic effect of intravenously administered autologous adipose-derived stem cells on chronic stage stroke patients. Reviewed
7 070 2020
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Coordination-Driven Self-Assembly on Polymer Surface for Efficient Synthesis of [Au(SPh)]n Coordination Polymer-Based Films
T. Tsuruoka, T. Ohhashi, J. Watanabe, R. Yamda, S. Hirao, T. Takashima, A. Demessence, S. Vaidya, O. Veselska, A. Fateeva, K. Akamatsu
Cryst. Growth 1961 - 1968 2020
Single Work
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Controlled syntheses of Ag nanoparticles inside MOFs by using amine-boranes as vapour phase reductants Reviewed
Yohei Takashima, Yasushi Sato, Takaaki Tsuruoka, Kensuke Akamatsu
Dalton Transactions 2020
Joint Work
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Efficacy of Fermented Extract Obtained from Multiple-Processed Fermentation Technology of Thermophilic Bacteria and Yeast Reviewed
Daichi Kawano, Zhengzheng Liao, Jing Nie, Akira Date, Eduardo Perez, Jose Fernandez, Corey Webb, Kristen Huber, Jeffry B. Stock, Junji Kawakami, Zihua Fu
JOURNAL OF JAPANESE COSMETIC SCIENCE SOCIETY 44 ( 3 ) 194 - 202 2020
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Different reactivity of Sp and Rp isomers of phosphorothioate-modified oligonucleotides in a duplex structure Reviewed
Md Ariful Islam, Aki Fujisaka, Junji Kawakami, Takao Yamaguchi, Satoshi Obika
Bioorg. Med. Chem. Lett. 30 ( 14 ) 127166 - 127166 2020
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Novel purification process for amyloid beta peptide(1-40) Invited Reviewed
Usui, K., Yokota, S.-I., Iwata, K., Hamada, Y.
Processes 8 2020
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Miyazaki, H., Samejima, Y., Iwata, K., Minamino, Y., Hikida, S., Ariumi, H., Ikeda, H., Hamada, Y., Yamashita, K., Usui, K.
International Journal of Molecular Sciences 21 2020
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Miyazaki, H., Takaishi, H., Ikeda, H., Ariumi, H., Hamada, Y., Yamashita, K., Usui, K.
Processes 8 2020
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Effect of tryptophan residues on gold mineralization by a gold reducing peptide Reviewed
Ozaki, M., Yoshida, S., Oura, M., Tsuruoka, T., Usui, K.
RSC Advances 10 2020
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Bioinspired cell nuclear nanotransporters generated by self‐assembly of amphiphilic polysaccharide‐amino acid derivatives conjugates Reviewed
Koji Nagahama, Yoshinori Sano, Mitsuo Inui, Seika Aoyama, Tokitaka Katayama, Kimika Ono
Advanced Biosystems 4 1900189 2020
Joint Work
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美容医療 new horizon －幹細胞への期待－ Invited Reviewed
市橋正光、長濱宏治
日本香粧品学会誌 44 30 - 35 2020
Joint Work
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細胞移植用インジェクタブルゲルの開発 Invited Reviewed
長濱宏治
Precision Medicine 3 724 - 728 2020
Single Work
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A thin hydrogel barrier linked onto cell surface sialic acids through covalent bonds induces cancer cell death in vivo Reviewed
Kimika Ono, Yuka Sanada, Yuka Kimura, Seika Aoyama, Natsumi Ueda, Tokitaka Katayama, Koji Nagahama
Biomaterials Science 8 577 - 585 2020
Joint Work
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Yohei Takashima, Yasushi Sato, Takaaki Tsuruoka, Kensuke Akamatsu
Dalton Transactions 49 ( 47 ) 17169 - 17172 2020
Joint Work
Publisher：Royal Society of Chemistry ({RSC})
<p>In this work, by using a series of amine–boranes as vapour phase reductants, we succeeded in synthesizing Ag nanoparticles in a controlled manner inside MOFs.</p>
DOI： 10.1039/D0DT03259J
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Effect of tryptophan residues on gold mineralization by a gold reducing peptide
Makoto Ozaki, Shuhei Yoshida, Maho Oura, Takaaki Tsuruoka, Kenji Usui
RSC Advances 2020
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Flexible and luminescent fibers of a 1D Au(i)–thiophenolate coordination polymer and formation of gold nanoparticle-based composite materials for SERS
Shefali Vaidya, Oleksandra Veselska, Antonii Zhadan, Marlène Daniel, Gilles Ledoux, Alexandra Fateeva, Takaaki Tsuruoka, Aude Demessence
Journal of Materials Chemistry C 2020
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Y. Takashima, Y. Sato, T. Tsuruoka, K. Akamatsu
Dalton Trans 49 ( 47 ) 17169 - 17172 2020
Joint Work
Authorship：Lead author, Corresponding author Publisher：Royal Society of Chemistry (RSC)
<p>In this work, by using a series of amine–boranes as vapour phase reductants, we succeeded in synthesizing Ag nanoparticles in a controlled manner inside MOFs.</p>
DOI： 10.1039/d0dt03259j
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Dermal fibroblasts internalize phosphatidylserine-exposed secretory melanosome clusters and apoptotic melanocytes. Reviewed
Ando, H., Yoshimoto, S., Yoshida, M., Shimoda, N., Tadokoro, R., Kohda, H., Ishikawa, M., Nshikata, T., Katayama, B., Ozawa, T., Tsuruta, D., Mizutani, K., Yagi, M. and Ichihashi, M.
Int J Mol Sci. 21 ( 5789 ) 2020
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Comparison of macrophage activation using γ-Globulin and serum-derived macrophage activating factor. Reviewed
12. Akamatsu, M., Mashiba, R., Kawakatsu, K., Ishikawa, M. and Nishikata, T.
Anticancer Res. 40 ( 8 ) 4707 - 4710 2020
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Improved Stability and Photodynamic Activity of Water-Soluble 5,15-Diazaporphyrins Incorporated in beta-(1,3-1,6)-d-Glucan with On-Off Switch Reviewed International journal
Shodai Hino, Shuhei Satake, Hideyuki Shinmori, Shigeki Kawabata, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Kouta Sugikawa, Riku Kawasaki, Atsushi Ikeda
CHEMISTRY-AN ASIAN JOURNAL 15 ( 3 ) 365 - 370 2019.12
Joint Work
Publisher：WILEY-V C H VERLAG GMBH
5,15-Diazaporphyrins, which have a large absorption at wavelengths over 600 nm, were dissolved in water by complex formation with beta-(1,3-1,6)-d-glucans. Aqueous solutions of these complexes were relatively stable compared with their trimethyl-beta-cyclodextrin-complexed analogues. beta-Glucan-complexed diazaporphyrins showed quenched fluorescence and had low singlet-oxygen-generation abilities owing to random self-aggregation. However, external stimuli, such as the presence of liposomes or intracellular uptake, restored the fluorescence and singlet-oxygen-generation abilities of beta-glucan-complexed diazaporphyrins. Consequently, beta-glucan-complexed diazaporphyrins showed very high photodynamic activities toward HeLa cells.
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Shikhar Tyagi, Sarika Saxena, Nikita Kundu, Taniya Sharma, Amlan Chakraborty, Sarvpreet Kaur, Daisuke Miyoshi, Jadala Shankaraswamy
RSC Advances 9 40255 - 40262 2019.12
Joint Work
We described a novel synthetic peptide in which a glutamine residue binds through hydrogen bonding to a guanine-base and a trytophan residue intercalates with K+ resulting in stabilization of a human telomeric G-quadruplex with high selectivity over its complementary c-rich strand and a double-stranded DNA and its complementary C-rich strand. This peptide offers great potential for cancer treatment by inhibiting the telomere extension by telomerase.
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Structural effect of amine N-oxides on the facilitation of alpha-glucosidase-catalyzed hydrolysis reactions Reviewed International journal
Takuma Aoki, Yuichi Nakagawa, Ryutaro Genjima, Kazuya Koumoto
BIOPROCESS AND BIOSYSTEMS ENGINEERING 43 ( 3 ) 541 - 548 2019.11
Joint Work
Publisher：SPRINGER
Activation and stabilization of enzymes is an important issue in their industrial application. We recently reported that synthetic betaines, derived from cellular metabolites, structure-dependently increased the activity and stability of various enzymes including hydrolases, oxidases, and synthetases simply by mixing them into the reaction buffer. In this report, we focus on amine N-oxides, which are similarly important metabolites in cells with a highly polarized N-oxide bond, and investigate their enzyme stabilization and activation behavior. It was revealed that synthetic amine N-oxides structure-dependently activate alpha-glucosidase-catalyzed hydrolysis reactions similarly to betaines. The subsequent comparison of the kinetic parameters, the optimal concentration range for activation, and the maximal activity, suggested that amine N-oxides facilitate hydrolysis reactions via the same mechanism as betaines, because no differences were confirmed. However, the enzyme stabilization effect of amine N-oxides was slightly superior to that of betaines and the temporal stability of the enzyme in aqueous solutions was higher in the low amine N-oxide concentration range. The rheological properties, CD spectra, and dynamic fluorescence quenching experiments suggested that the suppression of unfavorable conformational perturbation was related to the difference in the hydration environments provided by the surrounding water molecules. Thus, we clarified that amine N-oxides facilitate enzyme reactions as a result of their similarity to betaines and provide a superior stabilizing effect for enzymes. Amine N-oxides show potential for application in enzyme storage and long-term reactions.
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Turn-on fluorescence and photodynamic activity of beta-(1,3-1,6)-d-glucan-complexed porphyrin derivatives inside HeLa cells Reviewed International journal
Shodai Hino, Rikushi Funada, Kouta Sugikawa, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Atsushi Ikeda
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES 18 ( 12 ) 2854 - 2858 2019.11
Joint Work
Publisher：ROYAL SOC CHEMISTRY
Fluorescence intensities of water-soluble beta-(1,3-1,6)-d-glucan (beta-1,3-glucan)-complexed porphyrin derivatives were very weak as a result of self-quenching. However, beta-1,3-glucan-complexed tetra(aminophenyl)porphyrin exhibited 'off-state' to 'on-state' fluorescence switching activity by intracellular uptake. Furthermore, the internalised complex showed a high level of photodynamic activity toward HeLa cells under photoirradiation at long wavelengths.
DOI： 10.1039/C9PP00208A
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Bulky cations greatly increase the turnover of a native hammerhead ribozyme Reviewed
S. Nakano, H. Yamashita, K. Tanabe, N. Sugimoto
9 35820 - 35824 2019.11
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The RAS mRNA G-quadruplex: making undruggable cancer targets druggable
Keiko Kawauchi, Wataru Sugimoto, Daisuke Miyoshi
The Atlas of Science 2019.10
Joint Work
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DMPK is a New Candidate Mediator of Tumor Suppressor p53-Dependent Cell Death. Reviewed
Itoh K, Ebata T, Hirata H, Torii T, Sugimoto W, Onodera K, Nakajima W, Uehara I, Okuzaki D, Yamauchi S, Budirahardja Y, Nishikata T, Tanaka N, Kawauchi K.
Molecules 24 ( 17 ) E3175 2019.9
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Mechanical regulation of bone homeostasis through p130Cas-mediated alleviation of NF-κB activity. Reviewed International journal
Miyazaki T, Zhao Z, Ichihara Y, Yoshino D, Imamura T, Sawada K, Hayano S, Kamioka H, Mori S, Hirata H, Araki K, Kawauchi K, Shigemoto K, Tanaka S, Bonewald FL, Honda H, Shinohara M, Nagao M, Ogata T, Harada I, Sawada Y.
Science Adv. 5 2019.9
Joint Work
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Stabilization of DNA loop structures by large cations Reviewed
S. Nakano, T. Ayusawa, Y. Tanino, N. Sugimoto
J. Phys. Chem. B 123 7687 - 7694 2019.8
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研究課題設定を重視した科学技術人材育成プログラムROOTのデザインと実装
伊藤 真之, 臼井 英之, 長野 寛之, 巳波 弘佳, 甲元 一也, 蛯名 邦禎
日本科学教育学会年会論文集 43 399 - 400 2019.8
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ケミカルバイオロジー2.0
三好大輔, 山東信介, 清中茂樹, 花岡健二郎, 後藤佑樹
現代化学 581 18 - 25 2019.7
Joint Work
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Formation of Metal-Organic Frameworks on Metal Ion-Doped Polymer Substrate: In-Depth Time-Course Analysis using Scanning Electron Microscopy Reviewed
T. Tsuruoka, M. Hata, S. Hirao, T. Ohhashi, Y. Takashima, K. Akamatsu
Langmuir 35 10390 - 10396 2019.7
Joint Work
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Control of the nucleation and growth processes of metal-organic frameworks using a metal ion-doped polymer substrate for the construction of continuous films Reviewed
T. Ohhashi, T. Tsuruoka, Y. Takashima, K. Akamatsu
CrystEngComm 21 4851 - 4854 2019.7
Joint Work
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Characteristic morphological changes and rapid actin accumulation in serum-MAF-treated macrophages. Reviewed
Kawakatsu K, Ishikawa M, Mashiba R, Tran NK, Akamatsu M, Nishikata T
Anticancer Research 39 ( 8 ) 4533 - 4537 2019.6
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A case of chronic actinic dermatitis that responded completely to treatment with oral colostrum-macrophage activating factor (colostrum-MAF). Reviewed
Ichihashi M, Nakamura Y, Muto M, Nishikata T, Inui T, Uto Y.
Photodermatol Photoimmunol Photomed 2019.5
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細胞の分子夾雑環境における核酸構造と核酸構造リガンドの挙動 ～細胞内でも望みの機能を発現する分子の合理設計に向けて～
三好大輔
生命化学研究レター 58 ( 1 ) 17 - 21 2019.4
Single Work
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Enhancement of exon skipping activity by reduction in the secondary structure content of LNA-based splice-switching oligonucleotides Reviewed
T. Shimo, K. Tachibana, Y. Kawawaki, Y. Watahiki, T. Ishigaki, Y. Nakatsuji, T. Hara, J. Kawakami and S. Obika
Chem. Commun. 55 6850 - 6853 2019.4
Joint Work
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Integrated Synthesis of Gold Nanoparticles Coated with Polyoxometalate Clusters Reviewed
Sergio Martin, Yohei Takashima, Chang-Gen Lin, Yu-Fei Song, Haralampos N. Miras, Leroy Cronin
Inorganic Chemistry 58 ( 7 ) 4110 - 4116 2019.3
Joint Work
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Massive cytoplasmic transport and microtubule organization in fertilized chordate eggs. Reviewed
Nishikata T, Goto T, Yagi H, Ishii H.
Developmental Biology 448 ( 2 ) 154 - 160 2019.2
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Non-centrosomal microtubule structures regulated by egg activation signaling contribute to cytoplasmic and cortical reorganization in the ascidian egg. Reviewed
Goto T, Kanda K, Nishikata T.
Developmental Biology 448 ( 2 ) 161 - 172 2019.2
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Osmolyte-Enhanced Protein Synthesis Activity of a Reconstituted Translation System Reviewed
Yoshiki Moriizumi, Kazuhito V. Tabata, Daisuke Miyoshi, Hiroyuki Noji
ACS Synth Biol. 8 ( 3 ) 557 - 567 2019.2
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Mitochondrial protein E2F3d, a distinctive E2F3 product, mediates hypoxia-induced mitophagy in cancer cells. Reviewed International journal
Araki K, Kawauchi K, Sugimoto W, Tsuda D, Oda H, Yoshida R, Ohtani K.
Communications Biol. 6 550 - 561 2019.1
Joint Work
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Formation of Metal-Organic Frameworks on Metal Ion-Doped Polymer Substrate: In-Depth Time-Course Analysis using Scanning Electron Microscopy Reviewed
T. Tsuruoka, M. Hata, S. Hirao, T. Ohhashi, T. Takashima, K. Akamatsu
Langmuir 35 10390 - 10396 2019
Joint Work
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Control of the nucleation and growth processes of metal-organic frameworks using a metal ion-doped polymer substrate for the construction of continuous films Reviewed
T. Ohhashi, T. Tsuruoka, T. Takashima, K. Akamatsu
CrystEngComm 21 4851 - 4854 2019
Joint Work
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固液界面での選択的MOF形成
鶴岡孝章、平尾翔也、髙嶋洋平、赤松謙祐
日本接着学会誌 55 301 - 307 2019
Joint Work
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細胞移植用スマートゲルの開発 Invited
長濱宏治、小野公佳、木村友香、青山青海
ケミカルエンジニヤリング 64 17 - 26 2019
Joint Work
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Formation of Metal-Organic Frameworks on Metal Ion-Doped Polymer Substrate: In-Depth Time-Course Analysis using Scanning Electron Microscopy Reviewed
T. Tsuruoka, M. Hata, S. Hirao, T. Ohhashi, T. Takashima, K. Akamatsu
Langmuir 35 10390 - 10396 2019
Joint Work
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Control of the nucleation and growth processes of metal-organic frameworks using a metal ion-doped polymer substrate for the construction of continuous films Reviewed
T. Ohhashi, T. Tsuruoka, T. Takashima, K. Akamatsu
CrystEngComm 21 4851 - 4854 2019
Joint Work
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固液界面での選択的MOF形成
鶴岡孝章、平尾翔也、髙嶋洋平、赤松謙祐
日本接着学会誌 55 301 - 307 2019
Joint Work
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Metal sensitive and DNA concentration dependent structural rearrangement of short oligonucleotide into large suprastructures Reviewed
J. Shankaraswamy, Shikhar Tyagi, Anju Singh, Daisuke Miyoshi, Sarika Saxena
Journal of Biomolecular Structure and Dynamics 37 ( 9 ) 2211 - 2218 2018.12
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Wrapping Flexible Metal-Organic Framework with Organic Polymers via Site-Specific Radical Polymerization from Its Crystal Surface Invited Reviewed
Yohei Takashima, Kenji Fukudome, Atsuto Horikoshi, Takaaki Tsuruoka, Kensuke Akamatsu
Polyhedron 155 275 - 280 2018.11
Joint Work
Authorship：Lead author
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Yohei Takashima, Kenji Fukudome, Atsuto Horikoshi, Takaaki Tsuruoka, Kensuke Akamatsu
Polyhedron 155 275 - 280 2018.11
Joint Work
© 2018 Elsevier Ltd In this study, we succeeded in the crystal surface modification of metal–organic framework (MOF) with an organic polymer by site-specific radical polymerization. A MOF was selected with interpenetrated structure containing a naphthalenediimide moiety with electron accepting capability. N, N-dimethylaniline, as an electron donating guest, induced electron transfer to the napthalenediimide moiety, generating radical anions on the pore and crystal surfaces, initiating radical polymerization. The resulting composite was examined in detail with XRD, TG, TEM, ICP, IR, gas adsorption, and fluorescent measurements, demonstrating that an organic polymer was generated only on the crystal surface, preserving the original porous property. In addition, higher structural stability in response to H2O was observed after polymer wrapping.
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Anti-inflammatory effect of 5-aminolevulinic acid on macrophage in vitro. Reviewed
Ishikawa M, Mashiba R, Kawakatsu K, Nakagawa H, Kitajima Y, Ishizuka M, Nishikata T.
ALA-Porphyrin Science 7 ( 1 ) 3 - 11 2018.9
Joint Work
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Gold-Titania Nanocatalyst Generated by Mineralization Using Two Artificial Peptides with DNA Reviewed
Ozaki Makoto, Tomizaki Kin-Ya, Hamada Yoshio, Usui Kenji
JOURNAL OF PEPTIDE SCIENCE 24 S101 2018.9
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Effects of platinum and palladium nanocolloid on macrophage polarization in relevance to repigmentation of vitiligo. Reviewed
Minamiyama Y, Ishikawa M, Shibata T, Tsuji G, Nishikata T, Takemura S, Ichihashi M.
J Cutan Immunol Allergy 1 139 - 146 2018.8
Joint Work
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巻頭言 変化は突然に ―学会参加のススメ―
三好大輔
日本化学会 生体機能関連化学部会 ニュースレター 33 ( 2 ) 2 - 3 2018.8
Single Work
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Phagocytic activation of macrophages with serum MAF depends on engulfment efficiency and not migratory activity. Reviewed
Mashiba R, Ishikawa M, Sumiya YU, Kawakatsu K, Tran NK, Nishikata T.
Anticancer Res. 38 ( 7 ) 4295 - 4298 2018.7
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An anionic phthalocyanine decreases NRAS expression by breaking down its RNA G-quadruplex Reviewed
Keiko Kawauchi, Wataru Sugimoto, Takatoshi Yasui, Kohei Murata, Katsuhiko Itoh, Kazuki Takagi, Takaaki Tsuruoka, Kensuke Akamatsu, Hisae Tateishi-Karimata, Naoki Sugimoto, and Daisuke Miyoshi
Nature Communicationsvolume 9 2271 2018.6
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An anionic phthalocyanine decreases NRAS expression by breaking down its RNA G-quadruplex Reviewed International journal
Kawauchi K, Sugimoto W, Yasui T, Murata K, Itoh K, Takagi K, Tsuruoka T, Akamatsu K, Tateishi-Karimata H, Sugimoto N, Miyoshi D.
Nature Commun. 9 2271 2018.6
Joint Work
Authorship：Lead author, Corresponding author
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Living functional hydrogels generated by bioorthogonal cross-linking reactions of azide-modified cells with alkyne-modified polymers Reviewed
K. Nagahama, Y. Kimura, A. Takemoto
Nature Communications ( 9 ) 2195 2018.6
Joint Work
Authorship：Lead author
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Thermal stability and conformation of DNA and proteins under the confined condition in the matrix of hydrogels
S. Nakano, D. Yamaguchi, and N. Sugimoto
Mol. Biol. Rep. ４5 403 - 411 2018.4
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Kenji Usui, Shin-Ichiro Yokota, Makoto Ozaki, Shungo Sakashita, Takahito Imai, Kin-Ya Tomizaki
Protein and Peptide Letters 25 42 - 47 2018.4
Joint Work
Authorship：Lead author Publisher：Bentham Science Publishers B.V.
Background: A core sequence (the 9 C-terminal residues) of calcification-associated peptide (CAP-1) isolated from the exoskeleton of the red swamp crayfish was previously shown to control calcium carbonate precipitation with chitin. In addition, a modified core sequence in which the phosphorylated serine at the N terminus is replaced with serine exhibits was also previously shown to alter precipitation characteristics with chitin. Objectives: We focused on calcium carbonate precipitation and attempted to elucidate aspects of the mechanism underlying mineralization. We attempted to evaluate in detail the effects of modifying the N-terminus in the core sequence on calcium carbonate mineralization without chitin. Methods: The peptide modifications included phosphorylation, dephosphorylation, and a free or acetylated Nterminus. The peptides were synthesized manually on Wang resin using the DIPCI-DMAP method for the first residue, and Fmoc solid phase peptide synthesis with HBTU-HOBt for the subsequent residues. Prior to calcium carbonate precipitation, calcium carbonate was suspended in MilliQ water. Carbon dioxide gas was bubbled into the stirred suspension, then the remaining solid CaCO3 was removed by filtration. The concentration of calcium ions in the solution was determined by standard titration with ethylenediaminetetraacetate. Calcium carbonate precipitation was conducted in a micro tube for 3 h at 37°C. We used the micro-scale techniques AFM (atomic force microscopy) and TEM (transmission electron microscopy), and the macro-scale techniques chelate titration, HPLC, gel filtration, CD (circular dichroism) and DLS (dynamic light scattering). Results: We determined the morphologies of the calcium carbonate deposits using AFM and TEM. The pS peptide provided the best control of the shape and size of the calcium carbonate round particles. The acetylated peptides (Ac-S and Ac-pS) provided bigger particles with various shapes. S peptide provided a mixture of bigger particles and amorphous particles. We verified these findings using DLS. All the peptide samples produced nanostructures of the expected size in agreement with the AFM and TEM results. We estimated the abilities of these peptides to precipitate calcium carbonate by determining the residual calcium hydrogen carbonate concentration by standard titration with ethylenediaminetetraacetate after calcium carbonate precipitation. The Ac-pS peptide showed the lowest residual calcium hydrogen carbonate concentration whereas the S peptide showed the highest, suggesting that the precipitating activities of these peptides towards calcium carbonate correlated with peptide net charge. Then the gel filtration results showed a large oligomer peak and a small oligomer/monomer peak for all peptide samples in agreement with the AFM, TEM and DLS results. CD measurements showed that all the peptides formed random-coil-like structures. Thus, we used both macro-and micro-observation techniques such as chelate titration, DLS, AFM and TEM to show that the calcium carbonate precipitating activities of four derivatives of the core sequence of CAP-1 may correlate with the peptide net charge. Conclusion: These peptides mainly act as a catalyst rather than as a binder or component of the calcium carbonate deposits (as a template). On the other hand, the morphologies of the calcium carbonate deposits appeared to be dependent on the ability of the peptide to assemble and act as a template. Consequently, elucidating the relationship between peptide sequence and the ability of the peptide to assemble would be indispensable for controlling precipitate morphologies in the near future. This knowledge would provide important clues for elucidating the relationship between peptide sequence and mineralization ability, including deposit morphology and precipitating activity, for use in nanobiochemistry and materials chemistry research.
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Yuki Tominaga, Kenji Usui, Akiyoshi Hirata, Hiro-O Ito, Kiyoshi Nokihara
Bioorganic and Medicinal Chemistry 26 3210 - 3216 2018.4
Joint Work
Authorship：Lead author Publisher：Elsevier Ltd
A fundamental method has been developed focusing on a facile and rapid examination of periodontal disease. Periodontal disease is an oral disease thought to affect 80% of adults, and early detection with treatment is desirable for the improvement of the quality of life. Unfortunately conventional methods are not consistent as the disease is caused by a number of undefined bacteria and detection relies on the skills of the dentist. Thus an objective detection system is required. We have performed an experiment on saliva using a novel biodetection system, designated PepTenChip®. A disease model for saliva was prepared using a specimen from a healthy subject and a mixture of hemoglobin (f-Hb) and lactate dehydrogenase (LDH), which is used as a periodontal disease marker protein with healthy saliva. PepTenChip® is a peptide microarray in which fluorescent labelled structured peptides are immobilized on a novel amorphous carbon substrate. Since the peptides used as capture molecules are fluorescently labelled, labeling of analytes is not necessary. The fluorescence intensity change before and after application of analytes are detected rather than the ON/OFF detection common to conventional microarrays using a set of antigen–antibody. The fluorescence intensity value changes according to the concentration of captured protein allowing the generation of protein fingerprint (PFP) and dendrograms. The present method does not rely on a “one to one” interaction, unlike conventional biodetection, and advantages can be envisaged in the case of an undefined or unknown cause of disease. The statistical analyses, such as multivariate analyses, allow classification of the type of proteins added in saliva as mimetics of disease. PepTenChip® system is useful and convenient for examination of periodontal disease in health care.
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Peptides for Silica Precipitation: Amino Acid Sequences for Directing Mineralization Reviewed
Makoto Ozaki, Shungo Sakashita, Yoshio Hamada, Kenji Usui
Protein & Peptide Letters 25 15 - 24 2018.4
Joint Work
Publisher：Bentham Science Publishers B.V.
Background: Peptides are promising compounds for use in inorganic or organic-inorganic hybrid syntheses (mineralization) and offer several advantages over proteins. Meanwhile, silica-based nanomaterials have been extensively investigated for many years because of their potential application in a diverse range of technologies, including catalysis, sensing, separation, enzyme immobilization, and gene and drug delivery. Considerable progress has been made over the past decade in understanding the molecular mechanisms underpinning biosilicification and the biomimetic synthesis of patterned nanosilica using peptides. Objectives: This mini-review focuses on various peptide sequences, especially short peptide sequences (30 residues or less), for silica mineralization. Methods: We first briefly review early studies on silica mineralization using proteins to provide background information. This is followed by a discussion of promising peptide sequences and attempts to discern the relationship between amino acid sequence, their potential for mineralization, and the properties of the mineral product. Results: The synthetic control of silica mineralization using engineered proteins, such as recombinant silicateins and silaffins, was inspired by silica biomineralization by natural proteins from organisms (sponges, diatoms, and plants). Concurrently, several papers described the utility of well-structured protein assemblies as templates for silica mineralization. These template-directed syntheses of well-structured silica deposits were first conducted using natural proteins or protein assemblies such as collagen fibers and virus hollow protein tubes. Then we reviewed a selection of short peptides (30 residues or less) that had been successfully used for silica mineralization. Almost all peptides developed to date can be sorted by classification like proteins (synthetic control of silica mineralization or utility of templates for silica mineralization): the first class of peptides is used for peptide-directed synthesis, and the second is used for template-directed synthesis after the peptides have assembled and formed nanostructure such as fibers and tubes. The presented peptides were classified and arranged according to the classification. Additionally, we briefly introduced silica mineralization triggered by the combination of short silica-precipitating peptides and template molecules. Conclusion: In this mini-review we focused on various peptide sequences, especially short peptide sequences of 30 residues or less, designed for silica mineralization. The peptides have been used both for peptide-directed silica mineralization and for template-directed silica mineralization. The recent advances in peptide-driven mineralization reviewed here suggest that it will soon be possible to completely control the silica mineralization process using peptides. Mineralization systems using peptides will provide researchers with new tools for controlling various inorganic syntheses and the production of organic-inorganic materials for nanobiochemistry and materials chemistry research.
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Editorial: Organic-Inorganic Hybrid Materials and Their Applications Reviewed
Kin-ya Tomizaki, Yoshio Hamada, Kenji Usui
Protein & Peptide Letters 25 2 - 3 2018.4
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Substrate rigidity-dependent positive feedback regulation between YAP and ROCK2. Reviewed International journal
Sugimoto W, Itoh K, Mitsui Y, Ebata T, Fujita H, Hirata H, Kawauchi K
Cell adhesion & migration 12 ( 2 ) 101 - 108 2018.3
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Nanocomposite injectable gels capable of self-replenishing regenerative extracellular microenvironments for in vivo tissue engineering Reviewed
K. Nagahama, N. Oyama, K. Ono, A. Hotta, K. Kawauchi, T. Nishikata
Biomater Sci. 6 ( 3 ) 550 - 561 2018.2
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Nanocomposite injectable gels capable of self-replenishing regenerative extracellular microenvironments for in vivo tissue engineering Reviewed International journal
Koji Nagahama, Naho Oyama, Kimika Ono, Atsushi Hotta, Keiko Kawauchi, Takahito Nishikata
Biomater Sci. 6 550 - 561 2018.2
Joint Work
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A high-throughput quantitative assay system for macrophage phagocytic activity. Reviewed
Mami Ishikawa, Riho Mashiba, Kumpei Kawakatsu, Ngoc Kiet Tran, Takahito Nishikata
macrophage 2018.2
Joint Work
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Nanocomposite injectable gels capable of self-replenishing regenerative extracellular microenvironments for in vivo tissue engineering Reviewed
K. Nagahama, N. Oyama, K. Ono, A. Hotta, K. Kawauchi, T. Nishikata
Biomaterials Science ( 6 ) 550 - 561 2018.2
Joint Work
Authorship：Lead author
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Destabilization of DNA G-quadruplexes by chemical environment changes during tumor progression facilitates transcription Reviewed International journal
Hisae Tateishi-Karimata, Keiko Kawauchi, Naoki Sugimoto
J Am Chem Soc. 140 642 - 651 2018.1
Joint Work
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Crystal conversion between metal-organic frameworks with different crystal topologies for efficient crystal design on two-dimensional substrates Reviewed
T. Tsuruoka, K. Inoue, A. Miyanaga, K. Tobiishi, T. Ohhashi, M. Hata, Y. Takashima, K. Akamatsu
J. Cryst. Growth 487 1 - 7 2018
Joint Work
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ポリイミドフィルムの表面改質と銅薄膜のダイレクトめっき技術
髙嶋洋平、鶴岡孝章、赤松謙祐
MATERIAL STAGE 18 29 - 33 2018
Joint Work
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Wrapping Flexible Metal-Organic Framework with Organic Polymers via Site-Specific Radical Polymerization from Its Crystal Surface
Y. Takashima, K. Fukudome, A. Horikoshi, T. Tsuruoka, K. Akamats
Polyhedron 155 275 - 280 2018
Joint Work
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Controlling the Orientation of Metal−Organic Framework Crystals by an Interfacial Growth Approach Using a Metal Ion-Doped Polymer Substrate
T. Ohhashi, T. Tsuruoka, S. Fujimoto, Y. Takashima, K. Akamatsu
Cryst. Growth Des 18 402 - 408 2018
Joint Work
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Controlling the Orientation of Metal−Organic Framework Crystals by an Interfacial Growth Approach Using a Metal Ion-Doped Polymer Substrate
T. Ohhashi, T. Tsuruoka, S. Fujimoto, Y. Takashima, K. Akamatsu
Cryst. Growth Des 18 402 - 408 2018
Joint Work
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ポリイミドフィルムの表面改質と銅薄膜のダイレクトめっき技術
MATERIAL STAGE 18 29 - 33 2018
Single Work
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Wrapping Flexible Metal-Organic Framework with Organic Polymers via Site-Specific Radical Polymerization from Its Crystal Surface
Y. Takashima, K. Fukudome, A. Horikoshi, T. Tsuruoka, K. Akamatsu
Polyhedron 155 275 - 280 2018
Joint Work
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Design of anti-alzheimer’s disease agents focusing on a specific interaction with target biomolecules Reviewed
Yoshio Hamada, Kenji Usui
Neuromethods 132 207 - 228 2018
Joint Work
Publisher：Humana Press Inc.
Alzheimer’s disease (AD) is the most common cause of dementia, characterized by progressive intellectual deterioration. Amyloid β peptide (Aβ), the main component of senile plaques in the brains of patients with AD, is formed from amyloid precursor protein (APP) by two processing enzymes. According to the amyloid hypothesis, a processing enzyme β-secretase (BACE1
β-site APP cleaving enzyme) that triggers Aβ formation in the rate-limiting first step of Aβ processing appears to be a promising molecular target for therapeutic intervention in AD. Many researchers have revealed BACE1 inhibitors for the AD treatment. Early BACE1 inhibitors were designed based on the first reported X-ray crystal structure, 1FKN, of a complex between recombinant BACE1 and inhibitor OM99-2. Although OM99-2 seemed to interact with BACE1-Arg235 side chain by hydrogen bonding, we found that a quantum chemical interaction, such as σ-π interaction or π-π stacking, plays a critical role in BACE1 inhibition mechanism. Moreover, we proposed a novel “electron-donor bioisostere” concept in drug discovery study and designed potent BACE1 inhibitors using this concept. -
Channel current analysis estimates the pore-formation and the penetration of transmembrane peptides Reviewed
Sekiya, Y., Sakashita, S., Shimizu, K., Usui, K., Kawano, R.
Analyst 143 ( 15 ) 2018
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Crystal conversion between metal-organic frameworks with different crystal topologies for efficient crystal design on two-dimensional substrates Reviewed
T. Tsuruoka, K. Inoue, A. Miyanaga, K. Tobiishi, T. Ohhashi, M. Hata, Y. Takashima, K. Akamatsu
J. Cryst. Growth 487 1 - 7 2018
Joint Work
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Controlling the Orientation of Metal−Organic Framework Crystals by an Interfacial Growth Approach Using a Metal Ion-Doped Polymer Substrate Reviewed
T. Ohhashi, T. Tsuruoka, S. Fujimoto, Y. Takashima, K. Akamatsu
Cryst. Growth Des 18 402 - 408 2018
Joint Work
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Wrapping Flexible Metal-Organic Framework with Organic Polymers via Site-Specific Radical Polymerization from Its Crystal Surface
Y. Takashima, K. Fukudome, A. Horikoshi, T. Tsuruoka, K. Akamatsu
Polyhedron 155 275 - 280 2018
Joint Work
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ポリイミドフィルムの表面改質と銅薄膜のダイレクトめっき技術
髙嶋洋平、鶴岡孝章、赤松謙祐
MATERIAL STAGE 18 29 - 33 2018
Joint Work
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Controlling the Orientation of Metal-Organic Framework Crystals by an Interfacial Growth Approach Using a Metal Ion-Doped Polymer Substrate Reviewed
Takashi Ohhashi, Takaaki Tsuruoka, Seiya Fujimoto, Yohei Takashima, Kensuke Akamatsu
Cryst. Growth Des. 18 402 - 408 2018
Joint Work
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Crystal Conversion between Metal-Organic Frameworks with Different Crystal Topologies for Efficient Crystal Design on Two Dimensional Substrates Reviewed
Takaaki Tsuruoka, Kohei Inoue, Ayumi Miyanaga, Kaho Tobiishi, Takashi Ohhashi, Manami Hata, Yohei Takashima, Kensuke Akamatsu
J. Cryst. Growth 487 1 - 7 2018
Joint Work
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Tuning Light Emission towards White Light from a Naphthalenediimide-Based Entangled Metal-Organic Framework by Mixing Aromatic Guest Molecules Invited Reviewed
Rebeca Sola-Llano, Virginia Martínez-Martínez, Shuhei Furukawa, Yohei Takashima, Iñigo López-Arbeloa
Polymers 10 188 2018
Joint Work
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Modulation of the ribozyme and deoxyribozyme activities using tetraalkylammonium ions Reviewed
S. Nakano, T. Watabe, and N. Sugimoto
ChemPhysChem 18 ( 24 ) 3614 - 3619 2017.12
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生体分子の挙動解析研究を目標としたマイクロ波照射システムの開発 ～ペプチドのバイオミネラリゼーションにおけるマイクロ波影響解析をモデルとして～ Reviewed
臼井健二, 富樫浩行, 圓東那津実, 尾崎誠, 有本米次郎, 裏鍛武史, 大沢隆二, 皆木幸一, 中西伸浩, 梅谷智弘
日本電磁波エネルギー応用学会論文誌 17 - 24 2017.12
Joint Work
Authorship：Lead author
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Catalytic activities of ribozymes and DNAzymes in water and mixed aqueous media Invited Reviewed
S. Nakano, M. Horita, M. Kobayashi, and N. Sugimoto
Catalysis 7 ( 12 ) 355 - 348 2017.11
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DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity Reviewed
Kenji Usu, Arisa Okada, Shungo Sakashita, Masayuki Shimooka, Takaaki Tsuruoka, Shu-ichi Nakano, Daisuke Miyoshi, Tsukasa Mashima, Masato Katahira and Yoshio Hamada
Molecules 22 1991 2017.11
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Influence of intracellular environment on allosteric ribozyme activity
Misaki Kameno, Mika Sawada, Nae Sakimoto and Junji Kawakami
Proc. 44th Intl. Symp. Nucleic Acid Chemistry 178 - 179 2017.11
Joint Work
Authorship：Lead author
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Quantification of stabilization effect of co-solutes on RNA tertiary interaction
Natsumi Sasaki, Daisuke Miyoshi and Junji Kawakami
Proc. 44th Intl. Symp. Nucleic Acid Chemistry 174 - 175 2017.11
Joint Work
Authorship：Lead author
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Non-Covalent Loading of Anti-Cancer Doxorubicin by Modularizable Peptide Self-Assemblies for a Nanoscale Drug Carrier Reviewed
Kin-ya Tomizaki, Kohei Kishioka, Shunsuke Kataoka, Makoto Miyatani, Takuya Ikeda, Mami Komada, Takahito Imai, Kenji Usui
MOLECULES 22 ( 11 ) 1916 2017.11
Joint Work
Authorship：Lead author Publisher：MDPI AG
We prepared nanoscale, modularizable, self-assembled peptide nanoarchitectures with diameters less of than 20 nm by combining beta-sheet-forming peptides tethering a cell-penetrating peptide or a nuclear localization signal sequence. We also found that doxorubicin (Dox), an anti-cancer drug, was non-covalently accommodated by the assemblies at a ratio of one Dox molecule per ten peptides. The Dox-loaded peptide assemblies facilitated cellular uptake and subsequent nuclear localization in HeLa cells, and induced cell death even at low Dox concentrations. This peptide nanocarrier motif is a promising platform for a biocompatible drug delivery system by altering the targeting head groups of the carrier peptides.
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細胞環境化学模倣実験系におけるDNAの構造とその熱力学的安定性
三好大輔
日本核酸化学会誌 1 13 - 19 2017.10
Single Work
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Newly characterized interaction stabilizes DNA structure: oligoethylene glycols stabilize G-quadruplexes CH–π interactions Reviewed
H. Tateishi-Karimata, T. Ohyama, T. Muraoka, P. Podbevsek, A. M. Wawro, S. Tanaka, S. Nakano, K. Kinbara, J. Plavec, N. Sugimoto
Nucleic Acids Res. 45 7021 - 7030 2017.7
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Fluorescent and luminescent fusion proteins for analyses of amyloid beta peptide aggregation Reviewed
Kenji Usui, Masayasu Mie, Takashi Andou, Hisakazu Mihara, Eiry Kobatake
JOURNAL OF PEPTIDE SCIENCE 23 ( 7-8 ) 659 - 665 2017.7
Joint Work
Authorship：Lead author Publisher：WILEY
The amyloid beta (A) peptide is regarded as a causative agent of Alzheimer's disease. In this study, fluorescent and luminescent fusion proteins were constructed to analyze A aggregation. A system was developed to monitor changes in luminescence that provides information about A aggregation. In the presence of monomeric A, the fusion protein exhibits higher luminescence intensity, and the luminescence intensity is diminished after aggregation of the fusion protein and A. In contrast, the fluorescence is sustained in the presence of A. In the absence of A, the fusion protein self-aggregates, and its luminescence and fluorescence are quenched, thus decreasing the background fluorescence and enhancing the detection of A inside and outside the cells. The ratio of the luminescence intensity to the fluorescence intensity would allow the aggregation degrees of A to be distinguished. This study would be a promising method for analyzing the aggregation state of a particular amyloid protein/peptide (monomer, oligomer, or fibril), as well as the distribution of the amyloid protein/peptide within and at the cell surface, by using a single fusion protein. Copyright (c) 2017 European Peptide Society and John Wiley & Sons, Ltd.
DOI： 10.1002/psc.3003
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Quantitative relationship between chemical properties and bioactivities of anti-microRNA oligonucleotides targeted to tumor-associated microRNA-21 Reviewed
Koji Nagahama, Kenta Iseda, Daichi Kawano, Junji Kawakami
BIOCHIMIE 137 124 - 131 2017.6
Joint Work
Authorship：Last author, Corresponding author Publisher：ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Synthetic anti-microRNA oligonucleotides (AMOs) are promising drug candidates to inactivate disease related microRNAs because of their sequence-specific binding to their targets and the variety of chemical modifications available. Over the last decade, the qualitative relationships between the chemical properties of AMOs and bioactivity (inactivation of their target miRNAs) have been studied to enhance their bioactivity. On the other hand, in real-world drug development, drugs must be designed case-by case, taking many factors into account. Thus, in order to design AMOs that target specific miRNA, understanding the quantitative relationship between the chemical properties of AMOs and inactivation of their target miRNA is necessary. Here, we aimed to find the specific quantitative relationship of AMOs targeted to tumor-associated miR-21 through direct comparison of their inactivation efficacies with systematically varied chemical properties, including sequence-specific binding affinity, nuclease resistance, and RNase H activation. As a result, we newly found the quantitative relationships; (1) sequence specific binding affinity of AMOs against miR-21 is the main determining factor for inactivation efficacy, (2) nuclease resistance of AMOs impacts their miR-21 inactivation efficacy acting cooperatively with the binding affinity, although nuclease resistance alone does not affect the miRNA inactivation efficacy, and (3) RNase H activation is unnecessary. This study also demonstrates the utility of the obtained relationship for the design of AMO-based drugs targeted to miR-21, through cell-based analyses. Thus, the obtained quantitative relationship would make it possible to predict the miR-21 inactivation efficacy of AMOs which are newly designed. (C) 2017 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
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Quantitative relationship between chemical properties and bioactivities of anti-microRNA oligonucleotides targeted to tumor-associated microRNA-21 Reviewed
Koji Nagahama, Kenta Iseda, Daichi Kawano, Junji Kawakami
Biochimie 137 124 - 131 2017.6
Joint Work
Authorship：Lead author
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NF-kappaB prevents oncogenic Ras-induced b-actin cleavage in p53-deficient cells Reviewed
Wataru Sugimoto, Katsuhiko Itoh, Toshiya Kotari, Alvin K. Guo,Takahiro Ebata, Hiroaki Hirata, Keiko Kawauchi
International Journal of Cancer & Cellular Biology Research 2 ( 1 ) 15 - 18 2017.5
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Highly Sensitive Telomerase Assay Insusceptible to Telomerase and Polymerase Chain Reaction Inhibitors for Cervical Cancer Screening Using Scraped Cells. Reviewed
Hidenobu Yaku , Yoshio Yoshida, Hidehiko Okazawa, Yasushi Kiyono, Yuko Fujita, Daisuke Miyoshi
Anal. Chem. 89 ( 13 ) 6948 - 6953 2017.5
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PEG-based nanocomposite hydrogel: Thermoresponsive sol-gel transition controlled by PLGA-PEG-PLGA molecular weight and solute concentration Reviewed
Makoto Miyazaki, Tomoki Maeda, Kenji Hirashima, Naruki Kurokawa, Koji Nagahama, Atsushi Hotta
Polymer 115 246 - 254 2017.4
Joint Work
Authorship：Lead author
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Quantitative relationship between chemical properties and bioactivities of anti-microRNA oligonucleotides targeted to tumor-associated microRNA-21 Reviewed
Koji Nagahama, Kenta Iseda, Daichi Kawano, Junji Kawakami
Biochimie 137 124 - 131 2017.3
Joint Work
Authorship：Lead author
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Water Solubilization of Fullerene Derivatives by beta-(1,3-1,6)-D-Glucan and Their Photodynamic Activities toward Macrophages Reviewed International journal
Atsushi Ikeda, Tatsuya Iizuka, Naotake Maekubo, Kazuyuki Nobusawa, Kouta Sugikawa, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Motofusa Akiyama
CHEMISTRY-AN ASIAN JOURNAL 12 ( 10 ) 1069 - 1074 2017.3
Joint Work
Publisher：WILEY-V C H VERLAG GMBH
Anionic and neutral fullerene derivatives were dissolved in water by using beta-(1,3-1,6)-D-glucan (beta-1,3-glucan) as a solubilizing agent. In the water-solubilized complexes, the concentrations of fullerene derivatives were approximate to 0.30 mm and the average particle sizes were approximate to 90 nm. The beta-1,3-glucan-complexed fullerene derivative with a carboxylic acid was found to have higher photodynamic activity toward macrophages under visible-light irradiation (lambda > 610 nm) than other beta-1,3-glucan-complexed fullerene derivatives. This result suggests that carboxylic acid moieties in the complex enhance the binding affinity with beta-1,3-glucan receptors on the surface of macrophages when the beta-1,3-glucan is recognized. In contrast, all beta-1,3-glucan-complexed fullerene derivatives showed no photodynamic activity toward HeLa cells under the same conditions.
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Development of Peptide Microarray to Detect the Protein in Saliva for Periodontal Disease Test Reviewed
Yuki Tominaga, Kenji Usui, Akiyoshi Hirata, Atsushi Kitagawa, Hiro-O Ito and Kiyoshi Nokihara
Peptide Science 2016 131 - 132 2017.3
Joint Work
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Formation of beta-(1,3-1,6)-D-glucan-complexed [70]fullerene and its photodynamic activity towards macrophages Reviewed International journal
Atsushi Ikeda, Motofusa Akiyama, Kouta Sugikawa, Kazuya Koumoto, Yuta Kashijima, Jiawei Li, Toshio Suzuki, Takeshi Nagasaki
ORGANIC & BIOMOLECULAR CHEMISTRY 15 ( 9 ) 1990 - 1997 2017.2
Joint Work
Publisher：ROYAL SOC CHEMISTRY
[70] Fullerene was dissolved in water by complexation with beta-1,3-glucan using a mechanochemical highspeed vibration milling apparatus. The photodynamic activity of beta-1,3-glucan-complexed C-70 was highly dependent on the expression level of dectin-1 on the cell surfaces of macrophages. The photodynamic activity increased as a result of a synergistic effect between beta-1,3-glucan-complexed 1 '-acetoxychavicol acetate and the C-70 complex.
DOI： 10.1039/C6OB02747D
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Cell and Molecular Mechanics in Health and Disease Invited Reviewed
BioMed Research International Article ID 2860241 2017.2
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Nicotine-Selective Polymeric Adsorbent Obtained by Molecular Imprinting with Excess Use of Itaconic Acid Reviewed
Haruka Nogami, Yuma Nakahori, Takashi Murashima, Jun Matsui
Chromatography 38 15 - 21 2017.1
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Substrate stiffness influences doxorubicin- induced p53 activation via ROCK2 expression Reviewed
Ebata T, Mitsui Y, Sugimoto W, Maeda M, Araki K, Machiyama H, Harada I, Sawada Y, Fujita H, Hirata H, Kawauchi K
BioMed Research International 2017.1
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Unexpected Position-Dependent Effects of Ribose G‑Quartets in G‑Quadruplexes Reviewed
J. Zhou, S. Amrane, F. Rosu, G.F. Salgado, Y. Bian, H. Tateishi-Karimata, E. Largy, D. N. Korkut, A. Bourdoncle, D. Miyoshi, J. Zhang, H. Ju, W. Wang, N. Sugimoto, V. Gabelica, and J. Mergny
J. Am. Chem. Soc. 139 7768 - 7779 2017
Joint Work
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Selective and Robust Stabilization of Triplex DNA Structures Using Cationic Comb-type Copolymers. Reviewed
Yamayoshi A, Miyoshi D, Zouzumi YK, Matsuyama Y, Ariyoshi J, Shimada N, Murakami A, Wada T, Maruyama A
J Phys Chem B 121 ( 16 ) 4015 - 4022 2017
Joint Work
Authorship：Lead author
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Nicotine-selective Polymeric Adsorbent Obtained by Molecular Imprinting with Excess Use of Itaconic Acid Reviewed
Haruka Nogami, Yuma Nakahori, Takashi Murashima, Jun Matsui
Chromatography 38 ( 1 ) 15 - 21 2017
Authorship：Corresponding author
Currently, nicotine is mostly analyzed by chromatography with pretreatment such as solid phase extraction (SPE). One of effective pretreatment techniques would be affinity extraction; however, there is no practical biomolecular affinity media available for SPE of nicotine. Molecular imprinting has been studied as a methodology for producing nicotine-selective synthetic affinity media, which relies on the formation of complex species between a target molecule (as template) and functional monomer; therefore, the selection of functional monomer is greatly important for obtaining molecularly imprinted polymers (MIPs) with high affinity and selectivity. In this study, itaconic acid (IA), which bears two carboxyl groups, was used as functional monomer in the synthesis of nicotine selective-MIPs. Other acidic monomers, such as methacrylic acid (MA), 2-(trifluoromethyl)acrylic acid (FM) and methyl itaconate (MI) were compared with IA to evaluate their usefulness as functional monomer. In chromatographic tests, the retention factor for nicotine on a MIP synthesized with itaconic acid (IP-IA16) was 40.4, which was 3.1 times that on a non-imprinted polymer (BP-IA16), while the retention factor on MIPs with the other monomers was 21.6 or less and was 1.1 times that on corresponding non-imprint blank polymers (BPs). In the selectivity test using cotinine, 3-methylpyridine and N-methylpyrrolidine as reference compounds, IP-IA16 showed the largest retention factor for nicotine, which was more than 4.4 times that of the other compounds, suggesting that nicotine-recognition sites were formed in MIPs by the molecular imprinting using IA as functional monomer.
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Microtubule array observed in the posterior-vegetal cortex during cytoplasmic and cortical reorganization of the ascidian egg. Reviewed
Hirokazu Ishii, Toshiyuki Goto and Takahito Nishikata
Growth and Differentiation 59 648 - 656 2017
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An integrated function system using metal nanoparticle@mesoporous silica@metal-organic framework hybrids Reviewed
T. Ohhashi, T. Tsuruoka*, K. Inoue, Y. Takashima, S. Horike, K. Akamatsu
Micropor. Mesopor. Mater 245 104 - 108 2017
Joint Work
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Rational composition control of mixed-lanthanide metal-organic frameworks by an interfacial reaction with metal ion-doped polymer substrates Reviewed
T. Tsuruoka*, A. Miyanaga, T. Ohhashi, M. Hata, Y. Takashima, K. Akamatsu
J. Solid State Chem 253 43 - 46 2017
Joint Work
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An Integrated Function System Using Metal Nanoparticle@Mesoporous Silica@Metal-Organic Framework Hybrids
T. Ohhashi, T. Tsuruoka, K. Inoue, Y. Takashima, S. Horike, K. Akamatsu
Micropor. Mesopor. Mater 245 104 - 108 2017
Joint Work
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Rational composition control of mixed-lanthanide metal-organic frameworks by an interfacial reaction with metal ion-doped polymer substrates
T. Tsuruoka, A. Miyanaga, T. Ohhashi, M. Hata, Y. Takashima, K. Akamatsu
J. Solid State Chem 253 43 - 46 2017
Joint Work
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Ionic liquid/metal-organic framework hybrid generated by ion-exchange reaction: Synthesis and unique catalytic activity
Y. Takashima, M. Yokoyama, A. Horikoshi, Y. Sato, T. Tsuruoka, K. Akamatsu
New J. Chem 41 14409 - 14413 2017
Joint Work
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In situ generation and immobilization of an activated Rh complex catalyst in a metal-organic framework for hydrogenation under low H2 pressure
Y. Takashima, Y. Fukuhara, Y. Sato, T. Tsuruoka, K. Akamatsu
Eur. J. Inorg. Chem 45 5344 - 5349 2017
Joint Work
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Influence of intracellular environment on allosteric ribozyme activity
M. Kameno, M. Sawada, N. Sakimoto, J. Kawakami
Proc. 44th Intl. Symp. Nucleic Acid Chemistry 178 - 179 2017
Joint Work
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Quantification of stabilization effect of co-solutes on RNA tertiary interaction
N. Sasaki, D. Miyoshi, J. Kawakami
Proc. 44th Intl. Symp. Nucleic Acid Chemistry 174 - 175 2017
Joint Work
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An integrated function system using metal nanoparticle@mesoporous silica@metal-organic framework hybrids Reviewed
T. Ohhashi, T. Tsuruoka*, K. Inoue, Y. Takashima, S. Horike, K. Akamatsu
Micropor. Mesopor. Mater 245 104 - 108 2017
Joint Work
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Rational composition control of mixed-lanthanide metal-organic frameworks by an interfacial reaction with metal ion-doped polymer substrates Reviewed
T. Tsuruoka*, A. Miyanaga, T. Ohhashi, M. Hata, Y. Takashima, K. Akamatsu
J. Solid State Chem 253 43 - 46 2017
Joint Work
Authorship：Lead author
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An integrated function system using metal nanoparticle@mesoporous silica@metal-organic framework hybrids Reviewed
T. Ohhashi, T. Tsuruoka*, K. Inoue, Y. Takashima, S. Horike, K. Akamatsu
Micropor. Mesopor. Mater 245 104 - 108 2017
Joint Work
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Ionic Liquid/Metal-Organic Framework Hybrid Generated by Ion-Exchange Reaction: Synthesis and Unique Catalytic Activity Reviewed
Yohei Takashima*, Mai Yokoyama, Atsuto Horikoshi, Yasushi Sato, Takaaki Tsuruoka, Kensuke Akamatsu
New J. Chem. 41 14409 - 14413 2017
Joint Work
Authorship：Lead author
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In Situ Generation and Immobilization of an Activated Rh Complex Catalyst in a Metal-Organic Framework for Hydrogenation at Low H2 Pressure Reviewed
Yohei Takashima*, Yoshimasa Fukuhara, Yasushi Sato, Takaaki Tsuruoka, Kensuke Akamatsu
Eur. J. Inorg. Chem 45 5344 - 5349 2017
Joint Work
Authorship：Lead author
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Rational composition control of mixed-lanthanide metal-organic frameworks by an interfacial reaction with metal ion-doped polymer substrates Reviewed
T. Tsuruoka*, A. Miyanaga, T. Ohhashi, M. Hata, Y. Takashima, K. Akamatsu
J. Solid State Chem 253 43 - 46 2017
Joint Work
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Model studies of the effects of intracellular crowding on nucleic acid interactions Invited Reviewed
13 32 - 41 2016.12
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Elucidation of a “Signal-ON” Mechanism of Aggregation-Induced Emission Dye-Labelled DNA/DNA Duplexes and Application of a Repeat DNA Detection Method Reviewed
Takashi Murashima, Koji Kawamura, Ai Matsumoto
Medicinal Chemistry 6 704 - 709 2016.12
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Functional characterization of aconitase X as a cis-3-hydroxy-L-proline dehydratase Reviewed
Seiya Watanabe, Kunihiko Tajima, Satoshi Fujii, Fumiyasu Fukumori, Ryotaro Hara, Rio Fukuda, Mao Miyazaki, Kuniki Kino, Yasuo Watanabe
Scientific Reports 6 1 - 12 2016.12
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Unusual Colorimetric Change for Alkane Solvents with a Porous Coordination Framework
Takashima Yohei, Sato Yasushi, Tsuruoka Takaaki, Akamatsu Kensuke
INORGANIC CHEMISTRY 55 ( 22 ) 11617 - 11620 2016.11
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Effects of trimethylamine N-oxide and urea on DNA duplex and G-quadruplex Reviewed
Y. Ueda, Y. Zouzumi, A. Maruyama, S. Nakano, N. Sugimoto, and D. Miyoshi
Sci. Tech. Adv. Mat. 17 753 - 759 2016.11
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細胞核DDSのためのナノトランスポーターの設計 Invited Reviewed
長濱宏治
Drug Delivery System 31 482 - 483 2016.11
Single Work
Authorship：Lead author
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Lipid-membrane-incorporated arylboronate esters as agents for boron neutron capture therapy. Reviewed International journal
Masafumi Ueda, Kengo Ashizawa, Kouta Sugikawa, Kazuya Koumoto, Takeshi Nagasaki, Atsushi Ikeda
Organic & biomolecular chemistry 15 ( 7 ) 1565 - 1569 2016.10
Joint Work
Publisher：ROYAL SOC CHEMISTRY
Arylboronate esters bearing methyl groups in both of their ortho positions were stably incorporated into lipid membranes at high concentrations without undergoing hydrolysis to the corresponding boronic acids. This method could be used in combination with previous methods to increase the maximum ratio of boron atoms in liposomal boron carriers.
DOI： 10.1039/C6OB02142E
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Thermal stability of RNA structures with bulky cations in mixed aqueous solutions Reviewed
S. Nakano, Y. Tanino, H. Hirayama, and N. Sugimoto
Biophys. J. 111 1350 - 1360 2016.10
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MEKK1-dependent phosphorylation of calponin-3 tunes cell contractility. Reviewed
Hirata H, Ku WC, Yip AK, Ursekar CP, Kawauchi K, Roy A, Guo AK, Vedula SR, Harada I, Chiam KH, Ishihama Y, Lim CT, Sawada Y, Sokabe M
Journal of Cell Science 129 ( 19 ) 3574 - 3582 2016.10
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A Systematic Study on Molecular Mechanism of Pore-Forming Peptides for Discovering Antimicrobial Medicine
Y. Sekiya, H. Watanabe, K. Usui, R. Kawano
Proceedings of MicroTAS 2016 595 - 596 2016.10
Joint Work
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Increased yield of beta-glucosidase-catalyzed hydrolysis reactions in the presence of betaine-type metabolite analog Reviewed International journal
Shiro Sehata, Ryojun Suzuki, Kazuya Koumoto
BIOPROCESS AND BIOSYSTEMS ENGINEERING 40 ( 1 ) 153 - 159 2016.9
Joint Work
Publisher：SPRINGER
beta-Glucosidases (EC 3.2.1.21), abundant enzymes distributed in animals, plants and microorganism, has been generating lots of attentions for bioethanol production from cellulosic biomass. In this study, using three different origins of beta-glucosidases, glucose productivity of beta-glucosidase-catalyzed hydrolysis reactions in the presence of synthetic betaine-type metabolite analog (2-N,N,N-tri-n-butylammonium) acetate, was investigated. By the addition of the analog, the hydrolysis yields for all beta-glucosidases was highly improved from 4-13 to 64-100 %. To understand the factors affecting on the yield enhancements, the kinetic parameters, inhibition constants of end-product and temporal stability of beta-glucosidases were compared. As a result, enhancement of the yields is mainly related to the increase in the temporal stability of beta-glucosidases in the presence of the analog. The present findings lead to not only improve the glucose productivity of beta-glucosidase-catalyzed hydrolysis reaction toward bioethanol production but also apply to a new stabilization method for various unstable enzymes.
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Local thermodynamics of the water molecules around single- and double-stranded DNA studied by grid inhomogeneous solvation theory Reviewed
M. Nakano, H. Tateishi-Karimata, S. Tanaka, F. Tamab, O. Miyashita, S. Nakano, and N. Sugimoto
Chem. Phys. Lett. 660 ( 1 ) 250 - 255 2016.9
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Characterization of functional RNA to perform self-cleavage reaction under low pH condition
N. Sakimoto, M. Kameno, N. Sasaki, J. Kawakami
Proc. 43rd Intl. Symp. Nucleic Acid Chemistry 284 - 285 2016.9
Joint Work
Authorship：Lead author
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Discovery of a new function of curcumin which enhances its anticancer therapeutic potency Reviewed
Koji Nagahama, Tomoya Utsumi, Takayuki Kumano, Saeko Maekawa, Naho Oyama, Junji Kawakami
Scientific Reports 6 30962 2016.8
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Curcumisome nanovesicles generated by self-assembly of curcumin amphiphiles toward cancer theranostics Reviewed
K. Nagahama, T. Utsumi, T. Kumano, S. Maekawa, N. Oyama, J. Kawakami
Scientific Reports 6 30962 2016.8
Joint Work
Authorship：Lead author
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Macrophages Exhibit a Large Repertoire of Activation States via Multiple Mechanisms of Macrophage-activating Factors. Reviewed
Sumiya Y.U., Inoue T., Ishikawa M., Inui T., Kuchiike D., Kubo K., Uto Y., Nishikata T.
Anticancer Res. 36 ( 7 ) 3619 - 3623 2016.7
Joint Work
Authorship：Lead author
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Case Report: GcMAF Treatment in a Patient with Multiple Sclerosis. Reviewed
Inui T., Katsuura G., Kubo K., Kuchiike D., Chenery L., Uto Y., Nishikata T., Mette M.
Anticancer Res. 36 ( 7 ) 3771 - 3774 2016.7
Joint Work
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Case Report: A Non-small Cell Lung Cancer Patient Treated with GcMAF, Sonodynamic Therapy and Tumor Treating Fields. Reviewed
Inui T., Amitani H., Kubo K., Kuchiike D., Uto Y., Nishikata T., Mette M.
Anticancer Res. 36 ( 7 ) 3767 - 6770 2016.7
Joint Work
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Use of a ureido-substituted deoxycytidine module for DNA assemblies Invited Reviewed
S. Nakano, H. Oka, M. Fujii, and N. Sugimoto
Nucleosides, Nucleotides and Nucleic Acids 35 ( 7 ) 370 - 378 2016.5
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A reversible B-A transition of DNA duplexes induced by synthetic cationic copolymers Invited Reviewed
M. Yamaguchi, Y. Zouzumi, N. Shimada, S. Nakano, N. Sugimoto, A. Maruyama, and D. Miyoshi
Chem. Commun. 52 7446 - 7449 2016.4
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Site-Specific Control of Silica Mineralization on DNA Using a Designed Peptide Reviewed
M. Ozaki, K. Nagai, H. Nishiyama, T. Tsuruoka, S. Fujii, T. Endoh, T. Imai, K.-y. Tomizaki, K. Usui
Chem. Commun. 52 4010 - 4013 2016.3
Joint Work
Authorship：Lead author
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Site-specific control of silica mineralization on DNA using a designed peptide Reviewed
Makoto Ozaki, Kazuma Nagai, Hiroto Nishiyama, Takaaki Tsuruoka, Satoshi Fujii, Tamaki Endoh, Takahito Imai, Kin-ya Tomizaki, Kenji Usui
Chemical Communications 52 ( 21 ) 4010 - 4013 2016.3
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Development of Designed Peptides with Secondary Structure for Use in Nanobiotechnology Invited Reviewed
K.Usui
Peptide Science 2015 5 - 8 2016.3
Single Work
Authorship：Lead author
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The structural stability and catalytic activity of DNA and RNA oligonucleotides in the presence of organic solvents Invited Reviewed
S. Nakano and N. Sugimoto
Biophys. Rev. 8 ( 1 ) 11 - 23 2016.1
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Reevaluation of the stability of G-quadruplex structures under crowding conditions Reviewed
Jun Zhou, Hisae Tateishi-Karimata, Jean-Louis Mergny, Mingpan Cheng, Zhaochi Feng, Daisuke Miyoshi, Naoki Sugimoto, Can Li
Biochimie 121 204 - 208 2016
Joint Work
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A reversible B-A transition of DNA duplexes induced by synthetic cationic copolymers Reviewed
Nonoka Yamaguchi, Yu-ki Zouzumi, Naohiko Shimada, Shuichi Nakano, Naoki Sugimoto, Atsushi Maruyama, Daisuke Miyoshi
Chem. Commun. 52 7446 - 7449 2016
Joint Work
Authorship：Lead author
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In situ synthesis of Cu/Ni alloy nanoparticles embedded in thin polymer layers Reviewed
R. Shimizu, T. Kawakami, Y. Takashima, T. Tsuruoka, K. Akamatsu
RSC Adv 6 18895 - 18898 2016
Joint Work
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Morphology Control of Metal-Organic Frameworks Based on Paddle-Wheel Units on Ion-Doped Polymer Substrate Using An Interfacial Growth Approach Reviewed
T. Tsuruoka*, K. Mantani, A. Miyanaga, T. Matsuyama, T. Ohhashi, Y. Takashima, K. Akamatsu
Langmuir 32 6068 - 6073 2016
Joint Work
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Interfacial Synthetic Approach for Constructing Metal-Organic Framework Crystals Using Metal Ion-Doped Polymer Substrate Reviewed
T. Tsuruoka*, M. Kumano, K. Mantani, T. Matsuyama, A. Miyanaga, T. Ohhashi, Y. Takashima, H. Minami, T. Suzuki, K. Imagawa, K. Akamatsu
Cryst. Growth Des 16 2472 - 2476 2016
Joint Work
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Highly Sensitive Plasmonic Optical Sensors Based on Gold Core-Satellite Nanostructures Immobilized on Glass Substrates Reviewed
K. Ode, M. Honjo, Y. Takashima, T. Tsuruoka, K. Akamatsu
ACS Appl. Mater. Interfaces 8 20522 - 20526 2016
Joint Work
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Unusual Colorimetric Change for Alkane Solvents with Porous Coordination Framework Reviewed
Y. Takashima, Y. Sato, T. Tsuruoka, K. Akamatsu
Inorg. Chem 55 11617 - 11620 2016
Joint Work
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Site-selective growth of metal-organic frameworks using an interfacial growth approach combined with VUV photolithography Reviewed
T. Tsuruoka*, T. Matsuyama, A. Miyanga, T. Ohhashi, Y. Takashima, K. Akamatsu
RSC Adv 6 77297 - 77300 2016
Joint Work
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Highly Sensitive Plasmonic Optical Sensors Based on Gold Core−Satellite Nanostructures Immobilized on Glass Substrates
K. Ode, M. Honjo, Y. Takashima, T. Tsuruoka, K. Akamatsu
ACS Appli. Mater. Interfaces 8 20522 - 20526 2016
Joint Work
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Unusual Colorimetric Change for Alkane Solvents with a PorousCoordination Framework
Y. Takashima, Y. Sato, T. Tsuruoka, K. Akamatsu
Inorg. Chem 55 11617 - 11620 2016
Joint Work
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Site-Selective Growth of Metal–Organic Frameworks Using An Interfacial Growth Approach Combined with VUV Photolithography
T. Tsuruoka, T. Matsuyama, A. Miyanaga, T. Ohhashi, Y. Takashima, K. Akamatsu
RSC Advances 6 77297 - 77300 2016
Joint Work
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Morphology Control of Metal-Organic Frameworks Based on Paddle-Wheel Units on Ion-Doped Polymer Substrate Using An Interfacial Growth Approach
T. Tsuruoka, K. Mantani, A. Miyanaga, T. Matsuyama, T. Ohhashi, Y. Takashima, K. Akamatsu
Langmuir 32 6068 - 6073 2016
Joint Work
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Interfacial Synthetic Approach for Constructing Metal-Organic Framework Crystals Using Metal Ion-Doped Polymer Substrate
T. Tsuruoka, M. Kumano, K. Mantani, T. Matsuyama, A. Miyanaga, T. Ohhashi, Y. Takashima, H. Minami, T. Suzuki, K. Imagawa, K. Akamatsu
Cryst. Growth Des 16 2472 - 2476 2016
Joint Work
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In Situ Synthesis of Cu/Ni Alloy Nanoparticles Embedded in Thin Polymer Layers
R. Shimizu, T. Kawakami, Y. Takashima, T. Tsuruoka, K. Akamatsu
RSC Advances 6 18895 - 18898 2016
Joint Work
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Characterization of functional RNA to perform self-cleavage reaction under low pH condition
N. Sakimoto, M. Kameno, N. Sasaki, J. Kawakami
Proc. 43rd Intl. Symp. Nucleic Acid Chemistry 284 - 285 2016
Joint Work
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Discovery of a new function of curcumin which enhances its anticancer therapeutic potency
K. Nagahama, T. Utsumi, T. Kumano, S. Maekawa, N. Oyama, J. Kawakami
Scientific Reports 6 30962 2016
Joint Work
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A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis. Reviewed
Usui K, Tomizaki KY, Mihara H
Methods in molecular biology (Clifton, N.J.) 1352 199 - 210 2016
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Site-specific control of silica mineralization on DNA using a designed peptide Reviewed
Makoto Ozaki, Kazuma Nagai, Hiroto Nishiyama, Takaaki Tsuruoka, Satoshi Fujii, Tamaki Endoh, Takahito Imai, Kin-ya Tomizaki, Kenji Usui
CHEMICAL COMMUNICATIONS 52 ( 21 ) 4010 - 4013 2016
Publisher：ROYAL SOC CHEMISTRY
We developed a site-specific method for precipitating inorganic compounds using organic compounds, DNA, and designed peptides with peptide nucleic acids (PNAs). Such a system for site-specific precipitation represents a powerful tool for use in nanobiochemistry and materials chemistry.
DOI： 10.1039/c5cc07870a
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Site-specific control of multiple mineralizations using a designed peptide and DNA Reviewed
Kenji Usui, Makoto Ozaki, Aoi Yamada, Yoshio Hamada, Takaaki Tsuruoka, Takahito Imai, Kin-ya Tomizaki
NANOSCALE 8 ( 39 ) 17081 - 17084 2016
Publisher：ROYAL SOC CHEMISTRY
We have developed a site-specific method for precipitating multiple inorganic compounds using target DNA and a designed peptide consisting of a peptide nucleic acid (PNA) sequence and an inorganic compound-precipitating sequence. This system for controlled site-specific precipitation represents a powerful tool for use in nanobiotechnology and materials science.
DOI： 10.1039/c6nr03468c
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Site-specific control of silica mineralization on DNA using a designed peptide Reviewed
M. Ozaki, K. Nagai, H. Nishiyama, T. Tsuruoka, S. Fujii, T. Endoh, T. Imai, K. Tomizaki, K. Usui
Chem. Commun 52 4010 - 4010 2016
Joint Work
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Morphology Control of Metal-Organic Frameworks Based on Paddle-Wheel Units on Ion-Doped Polymer Substrate Using An Interfacial Growth Approach Reviewed
T. Tsuruoka*, K. Mantani, A. Miyanaga, T. Matsuyama, T. Ohhashi, Y. Takashima, K. Akamatsu
Langmuir 32 6068 - 6073 2016
Joint Work
Authorship：Lead author
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Interfacial Synthetic Approach for Constructing Metal-Organic Framework Crystals Using Metal Ion-Doped Polymer Substrate Reviewed
T. Tsuruoka, M. Kumano, K. Mantani, T. Matsuyama, A. Miyanaga, T. Ohhashi, Y. Takashima, H. Minami, T. Suzuki, K. Imagawa, K. Akamatsu
Cryst. Growth Des 16 2472 - 2476 2016
Joint Work
Authorship：Lead author
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In situ synthesis of Cu/Ni alloy nanoparticles embedded in thin polymer layers Reviewed
R. Shimizu, T. Kawakami, Y. Takashima, T. Tsuruoka, K. Akamatsu
RSC Adv 6 18895 - 18898 2016
Joint Work
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Unusual Colorimetric Change for Alkane Solvents with Porous Coordination Framework Reviewed
Y.Takashima, Y.Sato,
Inorg.Chem 55 11617 - 11620 2016
Single Work
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Site-specific control of multiple mineralizations using a designed peptide and DNA Reviewed
K. Usui, M. Ozaki, A. Yamada, Y. Hamada, T. Tsuruoka, T. Imai, K. Tomizaki
Nanoscale 8 17081 - 17084 2016
Joint Work
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Site-selective growth of metal-organic frameworks using an interfacial growth approach combined with VUV photolithography Reviewed
T. Tsuruoka*, T. Matsuyama, A. Miyanga, T. Ohhashi, Y. Takashima, K. Akamatsu
RSC Adv. 6 77297 - 77300 2016
Joint Work
Authorship：Lead author
-
Highly Sensitive Plasmonic Optical Sensors Based on Gold Core-Satellite Nanostructures Immobilized on Glass Substrates Reviewed
K. Ode, M. Honjo, Y. Takashima, T. Tsuruoka, K. Akamatsu
ACS Appl. Mater.Interfaces 8 20522 - 20526 2016
Joint Work
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Functions of the Tumor Suppressors p53 and Rb in Actin Cytoskeleton Remodeling.
Ebata T, Hirata H, Kawauchi K
BioMed research international 2016 9231057 2016
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In situ synthesis of Cu/Ni alloy nanoparticles embedded in thin polymer layers Reviewed
R. Shimizu, T. Kawakami, Y. Takashima, T. Tsuruoka, K. Akamatsu
RSC Adv 6 18895 - 18898 2016
Joint Work
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Shrink Wrapping Redox-Active Crystals of Polyoxometalate Open Frameworks with Organic Polymers via Crystal Induced Polymerisation Reviewed
Yohei Takashima, Haralampos N Miras, Stefan Glatzel, Leroy Cronin
Chem. Commun 52 7794 - 7797 2016
Joint Work
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Interfacial Synthetic Approach for Constructing Metal-Organic Framework Crystals Using Metal Ion-Doped Polymer Substrate Reviewed
T. Tsuruoka*, M. Kumano, K. Mantani, T. Matsuyama, A. Miyanaga, T. Ohhashi, Y. Takashima, H. Minami, T. Suzuki, K. Imagawa, K. Akamatsu
Cryst. Growth Des 16 2472 - 2476 2016
Joint Work
-
Morphology Control of Metal-Organic Frameworks Based on Paddle-Wheel Units on Ion-Doped Polymer Substrate Using An Interfacial Growth Approach Reviewed
T. Tsuruoka*, K. Mantani, A. Miyanaga, T. Matsuyama, T. Ohhashi, Y. Takashima, K. Akamatsu
Langmuir 32 6068 - 6073 2016
Joint Work
-
Highly Sensitive Plasmonic Optical Sensors Based on Gold Core-Satellite Nanostructures Immobilized on Glass Substrates Reviewed
K. Ode, M. Honjo, Y. Takashima, T. Tsuruoka, K. Akamatsu
ACS Appl. Mater. Interfaces 8 20522 - 20526 2016
Joint Work
-
Unusual Colorimetric Change for Alkane Solvents with Porous Coordination Framework Reviewed
Y. Takashima, Y. Sato, T. Tsuruoka, K. Akamatsu
Inorg. Chem 55 11617 - 11620 2016
Joint Work
Authorship：Lead author
-
Site-selective growth of metal-organic frameworks using an interfacial growth approach combined with VUV photolithography Reviewed
T. Tsuruoka*, T. Matsuyama, A. Miyanga, T. Ohhashi, Y. Takashima, K. Akamatsu
RSC Adv 6 77297 - 77300 2016
Joint Work
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Electrochemical quantification of the Alzheimer's disease amyloid-β(1-40) using amyloid-β fibrillization promoting peptide Reviewed
Satoshi Fujii, Daiki Sono, Kaoru Matsubara, Hitoshi Abe
Sensing and Bio-Sensing Research 6 7 - 12 2015.12
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Facile Quantification of Alzheimer's Disease Amyloid-β Based on Aggregation-Induced Emission Reviewed
Koji Kawamura, Runa Nakayama, Ai Matsumoto, Satoshi Fujii, Takashi Murashima
Journal of Pharmaceutical and Medicinal Research 1 ( 1 ) 27 - 30 2015.12
Joint Work
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ATP-Binding Peptide-Hydrogel Composite Synthesized by Molecular Imprinting on Beads Reviewed
Ayana Takata, Kenji Usui, Jun Matsui
Molecular Imprinting 3 65 - 70 2015.12
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Facile Quantification of Alzheimer’s Disease Amyloid-β Based On Aggregation-Induced Emission
Koji Kawamura, Runa Nakayama, Ai Matsumoto, Satoshi Fujii, Takashi Murashima
Journal of Pharmaceutical and Medicinal Research 1 ( 1 ) 27 - 30 2015.11
Joint Work
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Thermodynamic properties of water molecules in the presence of cosolute depend on DNA structure: a study using grid inhomogeneous solvation theory Reviewed
M. Nakano, H. Tateishi-Karimata, S. Tanaka, F. Tama, O. Miyashita, S. Nakano, and N. Sugimoto
Nucleic Acids Res. 43 10114 - 10125 2015.11
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A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis Reviewed
K. Usui, K.-y. Tomizaki, H. Mihara
Methods. Mol. Biol. 1352 199 - 210 2015.11
Joint Work
Authorship：Lead author
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p53 regulates cytoskeleton remodeling to suppress tumor progression.
Araki K, Ebata T, Guo AK, Tobiume K, Wolf SJ, Kawauchi K
Cellular and molecular life sciences : CMLS 72 ( 21 ) 4077 - 4094 2015.11
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Facile DNA Detection Based on Fluorescence Switching of A Hydrophobic AIE Dye-Labeled Peptide Nucleic Acid Probe by Aggregation/Disaggregation Reviewed
K. Kawamura, A. Matsumoto, T. Murashima
International Journal of Medical Nano Research 2 ( 2 ) 2015.9
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Cryoprotective ability of betaine-type metabolite analogs during freezing denaturation of enzymes International journal
Yuichi Nakagawa, Masahiro Sota, Kazuya Koumoto
BIOTECHNOLOGY LETTERS 37 ( 8 ) 1607 - 1613 2015.8
Publisher：SPRINGER
To evaluate an analog library of betaine-type cellular metabolites, which are naturally found in polar fish for survival in subzero temperatures, for preventing denaturation of enzymes during freezing.Comparison of the cryoprotective ability of reported cryoprotectants, such as dimethylsulfoxide, glycerol, ectoine, hydroxyectoine, and trehalose, with betaine-type analogs using alpha-glucosidase revealed that analogs introducing C-3-C-6 alkyl chains into an ammonium cation retained 20 % higher activity than the control cryoprotectants at the same concentration. In particular, the analog possessing triplicate n-butyl chains showed a profound effect. It allowed retention of enzyme activity to 95 % even after 100 freeze-thaw cycles, while addition of the control cryoprotectants decreased the activity to 10-20 %. The cryoprotective ability of betaine-type analogs can be applied not only to alpha-glucosidase but also other enzymes such as beta-glucosidase, alkaline phosphatase, lactose dehydrogenase, sulfatase, and horseradish peroxidase.Synthetic betaine-type metabolite analogs possess practicable cryoprotective ability for various enzymes, and are considerably superior to previously reported cryoprotectants.
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Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size Reviewed International journal
Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
BIOCONJUGATE CHEMISTRY 26 ( 8 ) 1775 - 1781 2015.8
Publisher：AMER CHEMICAL SOC
The design and synthesis of materials capable of activating the immune system in a safe manner is of great interest in immunology and related fields. Lactobacilli activate the innate immune system of a host when acting as probiotics. Here, we constructed lactobacilli-mimicking materials in which polysaccharide peptidoglycan complexes (PS PGs) derived from lactobacilli were covalently conjugated to the surfaces of polymeric microparticles with a wide variety of sizes, ranging from 200 nm to 3 mu m. The artificial lactobacilli successfully stimulated macrophages without cytotoxicity. Importantly, we found that the size of artificial lactobacilli strongly influenced their immunostimulating activities, and that artificial lactobacilli of 1 mu m exhibited 10-fold higher activity than natural lactobacilli. One major advantage of the artificial lactobacilli is facile control of size, which cannot be changed in natural lactobacilli. These findings provide new insights into the design of materials for immunology as well as the molecular biology of lactobacillus.
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Establishment of a macrophage-activating factor (MAF) assay system using human-monocytic cell line, THP-1. Reviewed
Inoue T., Ishikawa M., Sumiya Y., Kohda H, Inui T., Kuchiike D., Kubo K., Uto Y and Nishikata T.
Anticancer Research 35 4441 - 4445 2015.8
Joint Work
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Oral Colostrum Macrophage-activating Factor for Serious Infection and Chronic Fatigue Syndrome: Three Case Reports. Reviewed
Inui T., Kubo K., Kuchiike D., Uto Y., Nishikata T., Sakamoto N., Mette M.
Anticancer Research 35 ( 8 ) 4545 - 4549 2015.8
Joint Work
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Macrophage Activation Mechanisms in Human Monocytic Cell Line-derived Macrophages. Reviewed
Sumiya Y, Ishikawa M, Inoue T, Inui T, Kuchiike D, Kubo K, Uto Y, Nishikata T
Anticancer research 35 ( 8 ) 4447 - 4451 2015.8
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Establishment of a Macrophage-activating Factor Assay System Using the Human Monocytic Cell Line THP-1 Reviewed
Takahiro Inoue, Mami Ishikawa, Yu Sumiya, Haruka Kohda, Toshio Inui, Daisuke Kuchiike, Kentaro Kubo, Yoshihiro Uto, Takahito Nishikata
ANTICANCER RESEARCH 35 ( 8 ) 4441 - 4445 2015.8
Joint Work
Publisher：INT INST ANTICANCER RESEARCH
Background: As the mechanism of macrophage activation is not well-understood, standardization of an assay system for measuring phagocytic activities of macrophages will be useful for research on macrophages. Previously, we established a novel standardized macrophage-activating factor (MAF) assay system using U937. Materials and Methods: Using the human monocytic cell line THP-1, another standardized MAF assay system was established. Characteristic gene expression of U937- and THP-1-derived macrophages was compared by gene expression microarray analysis. Results: Both U937- and THP-1-derived macrophages showed obvious phagocytic activities with unique characteristics and, therefore, could not be assigned to a single sub-type. Conclusion: Activation of macrophages is an intricate cellular process. Comparison of our two novel assay systems provides new insights into macrophage activation mechanisms.
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Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size Reviewed
Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, and Jun Matsui
Bioconjugate Chemistry 26 1775 - 1781 2015.8
Joint Work
Authorship：Lead author
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Effects of background anionic compounds on the activity of the hammerhead ribozyme in Mg2+-unsaturated solutions Reviewed
S. Nakano, Y. Kitagawa, D. Miyoshi, and N. Sugimoto
J. Biol. Inorg. Chem 20 ( 6 ) 1049 - 1058 2015.7
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Effects of cosolvents on the folding and catalytic activities of the hammerhead ribozyme Reviewed
S. Nakano, Y. Kitagawa, H. Yamashita, D. Miyoshi, and N. Sugimoto
ChemBioChem 16 ( 12 ) 1803 - 1810 2015.7
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生命分子－合成高分子ハイブリッドの開発とナノバイオマテリアル，その可能性 Invited
長濱宏治
Materials Stage 7 18 - 21 2015.7
Single Work
Authorship：Lead author
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Anticancer drug-based multifunctional nanogels through self-assembly of dextran-curcumin conjugates toward cancer theranostics Reviewed
Koji Nagahama, Yoshinori Sano, Takayuki Kumano
Bioorganic & Medical Chemistry Letters 25 2519 - 2522 2015.6
Joint Work
Authorship：Lead author
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Effects of four-week feed restriction on toxicological parameters in beagle dogs. Reviewed
Takamatsu K., Yamashita H., Satake S., Kazusa K., Tabata H., and Nishikata T.
Experimental Animals 64 269 - 280 2015.5
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A mRNA-responsive G-quadruplex-based drug release system Reviewed
H. Yaku, T. Murashima, D. Miyoshi, N. Sugimoto
Sensors 15 ( 4 ) 9388 - 9403 2015.4
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Label and Label-Free Detection Techniques for Protein Microarrays. Reviewed International coauthorship
Syahir A, Usui K, Tomizaki KY, Kajikawa K, Mihara H
Microarrays (Basel, Switzerland) 4 ( 4 ) 228 - 244 2015.4
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DNA G-quadruplex detection system employing a protein fibril ligand Reviewed
R. Maeda, H. Yaku, T. Nakabayashi, T. Murashima, N. Sugimoto, D. Miyoshi
Telomere and Telomerase 2 2015.3
Joint Work
DOI： 10.14800/tt.691
Other Link： http://www.smartscitech.com/index.php/TT/article/view/691/pdf_6
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Mechanistic study of unusual enzyme activation caused by addition of inert betaine-type metabolite and the analogs
Yuichi Nakagawa, Kazuya Koumoto
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 249 2015.3
Publisher：AMER CHEMICAL SOC
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Self-Assembling Polymer Micelle/Clay Nanodisk/Doxorubicin Hybrid Injectable Gels for Safe and Efficient Focal Treatment of Cancer Reviewed
Koji Nagahama, Daichi Kawano, Naho Oyama, Ayaka Takemoto, Takayuki Kumano, Junji Kawakami
BIOMACROMOLECULES 16 ( 3 ) 880 - 889 2015.3
Joint Work
Publisher：AMER CHEMICAL SOC
The purpose of this study was to fabricate a safe and effective doxorubicin (DOX)-delivery system for focal cancer chemotherapy. A novel biodegradable injectable gel was developed through self-assembly of poly(D,L-lactide-co-glycolide)-b-poly(ethylene glycol)-b-poly(D,L-lactide-co-glycolide) (PLGA-PEG-PLGA) copolymer micelles, clay nanodisks (CNDs), and DOX. We discovered that DOX loaded in the hybrid gels acts as an anticancer drug and as a building block to organize new gel networks. Accordingly, long-term sustained release of DOX from hybrid injectable gels without initial burst release was achieved. Moreover, it was revealed that the DOX incorporated into gel networks controls its own release profile. This hybrid injectable gel is a self-controlled drug release system, which is a novel concept in controlled drug release. Importantly, a single injection of PLGA-PEG-PLGA/CND/DOX hybrid gel provides long-term sustained antitumor activity in vivo against human xenograft tumors in mice, suggesting the potential of hybrid gels as a valuable local DOX-delivery platform for cancer focal therapy.
DOI： 10.1021/bm5017805
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Site-Specific Mineralization of Silica and Calcium on DNAs Using a Designed Peptide Reviewed
K. Usui, H. Nishiyama, A. Yamada, M. Ozaki, T. Tsuruoka, K.-y. Tomizaki
Peptide science 2014 325 - 326 2015.3
Joint Work
Authorship：Lead author
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Self-Assembling Polymer Micelle/Clay Nanodisk/Doxorubicin Hybrid Injectable Gels for Safe and Efficient Focal Treatment of Cancer Reviewed
Koji Nagahama, Daichi Kawano, Naho Oyama, Ayaka Takemoto, Takayuki Kumano, Junji Kawakami
Biomacromolecules 16 880 - 889 2015.3
Joint Work
Authorship：Lead author
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Synthesis and lmmunestimulating Activity of Lactobacilli-Originated Polysaccharide-Polymeric Microparticle Conjugates Reviewed International journal
Koji Nagahama, Takayuki Kumano, Tsubasa Nakata, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
LANGMUIR 31 ( 4 ) 1489 - 1495 2015.2
Publisher：AMER CHEMICAL SOC
The design and synthesis of biomaterials capable of activating the immune system are of interest in immunology-related fields because of their ability to tune up the immune defenses of the host. Lactobacilli are a major constituent of normal human indigenous flora, and some specific strains are known to activate the immune system of the host as probiotics. In this study, we first fabricated novel biohybrid materials in which lactobacilli (L. casei strain Shirota, LcS)-originated polysaccharide-peptidoglycan complexes (PS-PGs) are conjugated with polymeric microparticles (MPs). PS-PGs conjugated onto polymeric MPs surfaces bound its specific antibody, suggesting that PS-PGs kept their original molecular recognition ability. The PS-PGs-based hybrid MPs with an appropriate density of conjugated PS-PGs effectively induced high levels of IL-12 production from macrophages without cytotoxicity. These results suggest that LcS-originated PS-PGs could be available bio-originated materials for developing novel biomaterials capable of activating the immune system in a safe manner.
DOI： 10.1021/la5041757
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Synthesis and Immunestimulating Activity of Lactobacilli-Originated Polysaccharide-Polymeric Microparticle Conjugates Reviewed
Koji Nagahama, Takayuki Kumano, Tsubasa Nakata, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, and Jun Matsui
Langmuir 31 1489 - 1495 2015.1
Joint Work
Authorship：Lead author
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Synthesis and Immunestimulating Activity of Lactobacilli-Originated Polysaccharide-Polymeric Microparticle Conjugates Reviewed
Koji Nagahama, Takayuki Kumano, Tsubasa Nakata, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
Langmuir 31 1489 - 1495 2015
Joint Work
Authorship：Lead author
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Cryoprotective ability of betaine-type metabolite analogs during freezing denaturation of enzymes Reviewed
Yuichi Nakagawa, Masahiro Sota, Kazuya Koumoto
Biotechnology Letters 37 1607 - 1613 2015
Joint Work
Authorship：Lead author
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Immunestimulating Activity of Lactobacilli-mimicking Microparticles modified withPolysaccharide-Peptidoglycan Complex derived from L. casei Shirota Strain Invited
Kazuya Koumoto, Koji Nagahama, Jun Matsui
BIOINDUSTRY 32 ( 9 ) 42 - 47 2015
Joint Work
Authorship：Lead author
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Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size Reviewed
Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
Bioconjugate Chemistry 26 ( 8 ) 1775 - 1781 2015
Joint Work
Authorship：Lead author
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Significance of anionic functional group in betaine-type metabolite analogs on the facilitation of enzyme reactions International journal
Yuichi Nakagawa, Kotomi Takagi, Ryutaro Genjima, Kazuya Koumoto
BIOPROCESS AND BIOSYSTEMS ENGINEERING 38 ( 9 ) 1811 - 1817 2015
Joint Work
Authorship：Lead author Publisher：SPRINGER
Using synthetic sulfobetaine library, the enzyme activation behavior has been investigated. Comparison of enzyme activation behavior revealed that sulfobetaines equally facilitate enzyme reactions, being consistent with that of carboxybetaines. The subsequent kinetic and solution property analyses clarified that both the kinetic parameter and hydration property changes are identical with those of carboxybetaines, indicating that the difference in the anionic functional group of the betaine structure scarcely affects the enzyme activation. On the other hand, comparison of carboxy- or sulfo-betaines with tetraalkylammonium salts, whose counteranion binds to the ammonium cation intermolecularly, revealed that the activation ability for enzymes of tetraalkylammonium salts is considerably smaller than that of carboxy- or sulfo-betaines. These findings give us a hint to design the useful betaine-type enzyme activators.
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Enhanced Chromogenic Sensitivity of Horseradish Peroxidase-Catalyzed Oxidative Reactions in the Presence of Betaine-Type Metabolite Analogs Reviewed
Kotomi Takagi, Yasuhiro Kashima, Satoshi Fujii, Kazuya Koumoto
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 88 ( 8 ) 1074 - 1082 2015
Joint Work
Authorship：Lead author Publisher：CHEMICAL SOC JAPAN
Horseradish peroxidase (HRP), a well-known oxidase, is frequently used in the diagnostic field as a labeling enzyme, where it amplifies the substrate binding signal of antibodies. Though practical for detecting moderate amounts of substrate, there is strong demand for further development of its sensitivity to detect minuscule quantities of biomarkers. Recently, we found that betaine-type cellular metabolite analogs facilitate enzymatic hydrolysis just by dissolving them into the reaction buffer. In the present study, using the analog (2-(N,N,N-tri-n-butylammonium) acetate) and various colorimetric substrates of HRP, we investigated the activation behavior of HRP. As a result, the analog structure- and concentration-dependently facilitated the various HRP-catalyzed oxidative reactions. Interestingly, the analog facilitated not only the reaction rate but also the chromogenic sensitivity. Kinetic and structural analyses revealed that the increased chromogenic sensitivity is related to the enhancement of the conformational flexibility in the substrate binding site in HRP by addition of the analog, which diminishes the binding affinity between HRP and large substrates. The finding serves to create practical applications of the analogs for increased detection sensitivity of HRP-related clinical agents.
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DNA structures under molecular crowding conditions with a phosphorylcholine derivative (MPC) Reviewed
Yu-mi Ueda, Tomohiro Konno, Kazuhiko Ishihara, Naoki Sugimoto, Daisuke Miyoshi
Transact. Mat. Res. Soc. Japan 40 99 - 102 2015
Joint Work
Authorship：Lead author
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Effects of background anionic compounds on the activity of the hammerhead ribozyme in Mg2+-unsaturated solutions Reviewed
Shu-ichi Nakano, Yuichi Kitagawa, Daisuke Miyoshi, Naoki Sugimoto
J. Biol. Inorg. Chem. 20 1049 - 1058 2015
Joint Work
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Stabilization of DNA Structures With Poly(ethylene sodium phosphate) Reviewed
R. Moriyama, Y. Iwasaki, D. Miyoshi
J. Phys. Chem. B 119 11969 - 11977 2015
Joint Work
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Effects of cosolvents on the folding and catalytic activities of the hammerhead ribozyme Reviewed
S. Nakano, Y. Kitagawa, H. Yamashita, D. Miyoshi, N. Sugimoto
ChemBioChem 16 1803 - 1810 2015
Joint Work
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A mRNA-responsive G-quadruplex-based drug release system Invited Reviewed
Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, and Naoki Sugimoto
Sensors 15 ( 4 ) 9388 - 9403 2015
Joint Work
Authorship：Lead author
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DNA G-quadruplex detection system employing a protein fibril ligand Invited Reviewed
Ryuichi Maeda, Hidenobu Yaku, Takakazu Nakabayashi, Takashi Murashima, Naoki Sugimoto, Daisuke Miyoshi
Telomere and Telomerase 2 e691 2015
Joint Work
Authorship：Lead author
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DNA structures under molecular crowding conditions with a phosphorylcholine derivative (MPC)
Yu-mi Ueda, Tomohiro Konno, Kazuhiko Ishihara, Naoki Sugimoto, Daisuke Miyoshi
Transact. Mat. Res. Soc. Japan 40 99 - 102 2015
Joint Work
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Stabilization of DNA Structures With Poly(ethylene sodium phosphate)
R. Moriyama, Y. Iwasaki, D. Miyoshi
J. Phys. Chem. B 119 11969 - 11977 2015
Joint Work
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Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface Reviewed
T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu
J. Colloid Interface Sci 451 212 - 215 2015
Joint Work
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Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface Reviewed
T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu
J. Colloid Interface Sci 451 212 - 215 2015
Joint Work
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Metal Nanocrystal/Metal Organic Framework Core/Shgell Nanostructure From Selective Self-Assembly Induced By Localization of Metal Ion Precursors on Nanocrystal Surface
T. Ohhashi, T. Tsuruoka, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu
J. Colloid Interface Sci 451 212 - 215 2015
Joint Work
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Comparison of Kinetic and Thermodynamic Analyses for Duplex Formation
E. Tomita, M. Sumimoto, S. Itoh, M. Takashima, and J. Kawakami
Proc. 42nd Intl. Symp. Nucleic Acid Chemistry 142 - 143 2015
Joint Work
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Curcumisome nanovesicles generated by self-assembly of curcumin amphiphiles toward cancer theranostics
Koji Nagahama, Takayuki Kumano, Naho Oyama, Junji Kawakami
BIOMATERIALS SCIENCE 3 ( 12 ) 1566 - 1578 2015
Joint Work
Publisher：ROYAL SOC CHEMISTRY
Curcumin (CCM) is an important molecule for achieving cancer theranostics because CCM is a naturally-occurring biocompatible material that exhibits both anticancer activity and strong fluorescence property that can be used for bio-imaging. However, CCM has never been utilized in clinical trials due to its extremely low water solubility, its rapid hydrolysis in aqueous conditions at neutral pH, and its low cellular uptake into cancer cells. Taking advantage of the strong hydrophobicity, p-conjugated frameworks, and ketone and enol groups that generate hydrogen bonds in CCM, we herein fabricated novel CCM-based biodegradable nanovesicles, which we termed as "curcumisome", through the self-assembly of amphiphilic CCM-poly(ethylene glycol) conjugates in aqueous media to develop multifunctional nanobiomaterials for use in cancer theranostics. A high CCM loading content in the curcumisomes was achieved, and the curcumisomes showed high water dispersibility with improved hydrolysis resistance. Importantly, the curcumisomes were effectively internalized into cancer cells and exhibited strong fluorescence for a long period, which is favorable for cancer cell imaging, although only a small amount of the curcumisomes penetrated into normal cells and showed very weak fluorescence. Moreover, curcumisomes effectively induced apoptosis of cancer cells. Thus, curcumisomes may act as multifunctional nanobiomaterials for the development of CCM-based cancer theranostics.
DOI： 10.1039/c5bm00212e
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Control of guanine-rich DNA secondary structures depending on the protease activity using a designed PNA peptide Reviewed
Kenji Usui, Arisa Okada, Keita Kobayashi, Naoki Sugimoto
ORGANIC & BIOMOLECULAR CHEMISTRY 13 ( 7 ) 2022 - 2025 2015
Publisher：ROYAL SOC CHEMISTRY
We constructed a regulation system for DNA secondary structure formation of G-rich sequences using a designed PNA peptide exhibiting an on-to-off switching functionality, depending on the protease activity. This study introduces the new concept of a simple and powerful system for regulating quadruplex-related important biological events.
DOI： 10.1039/c4ob02535k
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Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface Reviewed
T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu
J. Colloid Interface Sci. 451 212 - 215 2015
Joint Work
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Site-Specific Mineralization of Silica and Calcium on DNAs Using a Designed Peptide Reviewed
K. Usui, H. Nishiyama, A. Yamada, M. Ozaki, T. Tsuruoka, K.-y. Tomizaki
Peptide Science 325 - 326 2015
Joint Work
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Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface Reviewed
T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu
J. Colloid Interface Sci 451 212 - 215 2015
Joint Work
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Direct Fabrication of Metal Patterns on Resin Substrate by Combining Imprint and Electrochemical Lithography Reviewed
Yusuke Kimura, Ryosuke Fujiwara, Takaaki Tsuruoka, Yohei Takashima, Hidemi Nawafune, Hiroshi Yanagimoto, Kensuke Akamatsu
J. Surf. Finish. Soc. Jpn 66 18 - 19 2015
Joint Work
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Dependence of Crystal Size on the Catalytic Performance of a Porous Coordination Polymer Reviewed
Tomokazu Kiyonaga, Masakazu Higuchi, Takashi Kajiwara, Yohei Takashima, Jingui Duan, Kazuro Nagashima, Susumu Kitagawa
Chem. Commun 51 2728 - 2730 2015
Joint Work
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Preparation of hydrogels for the study of the effects of spatial confinement on DNA Reviewed
S. Nakano, M. Yoshida, D. Yamaguchi, and N. Sugimoto
Trans. Mat. Res. Soc. Jpn. 39 435 - 438 2014.12
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A novel ribozyme core to perform self-cleavage reaction
S. Tanaka, N. Sakimoto, M. Kameno, N. Sasaki, and J. Kawakami
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 340 - 341 2014.11
Joint Work
Authorship：Lead author
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Thermodynamic Features Provided by an ENA Introduction to Nucleic Acid Drugs
E. Tomita, M. Sumimoto, and J. Kawakami
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 188 - 189 2014.11
Joint Work
Authorship：Lead author
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Effects of oligonucleotide length and locked nucleic acid content on splice-switching oligonucleotide activity
T. Shimo, K. Tachibana, K. Saito, T. Yoshida, E. Tomita, R. Waki, T. Yamamoto, T. Doi, T. Inoue, J. Kawakami, and S. Obika
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 332 - 333 2014.11
Joint Work
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Understanding p53: new insights into tumor suppression.
Kawauchi K, Wolf SJ
Expert review of anticancer therapy 14 ( 10 ) 1101 - 1103 2014.10
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Mechanistic study on the facilitation of enzymatic hydrolysis by alpha-glucosidase in the presence of betaine-type metabolite analogs Reviewed
Yuichi Nakagawa, Shiro Sehata, Satoshi Fujii, Hiroaki Yamamoto, Akihiko Tsuda, Kazuya Koumoto
TETRAHEDRON 70 ( 35 ) 5895 - 5903 2014.9
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
Recently we reported that betaine-type metabolite analogs structure-dependently facilitate enzymatic hydrolysis reaction for alpha-glucosidases, beta-glucosidases, and alkaliphosphatases. To understand the facilitation mechanism for enzymes, in this study we expanded the analog library and measured the properties of analog solutions. The structural investigation on alpha-glucosidase-mediated hydrolysis reaction indicated that suitable structures to facilitate the enzyme reaction efficiently should have the ammonium cation in the betaine structure possess triplicate aliphatic chains from Cl to C7 without any polar functional groups. Analyses of the solution properties revealed that such analogs possess a large hydration layer with low water density. Such a specific hydration environment is generated by the characteristic structure of the betaine-type metabolite analogs. The characteristic hydration indirectly regulates enzyme activity and stability. These findings not only increase our understanding enzyme activation by betaine-type metabolite analogs, but also will contribute to the molecular design of enzyme regulators. (C) 2014 Elsevier Ltd. All rights reserved.
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Refinement and verification of test conditions for colony-forming unit assay in dog bone marrow cells with preservable colony specimens. Reviewed
Takamatsu K., Wakata A., Hirota R., Tabata H., and Nishikata T.
Comparative Clinical Pathology 23 ( 5 ) 1133 - 1139 2014.9
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Cellular differentiation assessments by measuring the degree of cellular internalization and membrane adsorption using designed peptides Reviewed
Kenji Usui, Takuya Kikuchi, Kunio Kikuchi, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 24 ( 17 ) 4129 - 4131 2014.9
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
We demonstrate examples of cellular differentiation assessments, including cellular neurite outgrowth and fat cell maturation, by measuring the degree of membrane adsorption or cellular internalization using designed peptides. Because changes in the cellular membrane and cytosol during differentiation were shown to influence membrane adsorption and cellular internalization, we could successfully evaluate the extent of differentiation simply like stain indicators. (C) 2014 Elsevier Ltd. All rights reserved.
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Roles of the amino group of purine bases in the thermodynamic stability of DNA base pairing Reviewed
S. Nakano and N. Sugimoto
Molecules 19 ( 8 ) 11613 - 11627 2014.8
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Case Report: A Breast Cancer Patient Treated with GcMAF, Sonodynamic Therapy and Hormone Therapy. Reviewed
Inui T, Makita K, Miura H, Matsuda A. Kuchiike D, Kubo K, Mette M, Uto Y, Nishikata T, Hori H, and Sakamoto N.
Anticancer Research 34 ( 8 ) 4589 - 4593 2014.8
Joint Work
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Dangling ends perturb the stability of RNA duplexes responsive to surrounding conditions Reviewed
H. Tateishi-Karimata, S. Pramanik, S. Nakano, D. Miyoshi, and N. Sugimoto
ChemMedChem 9 ( 9 ) 2150 - 2155 2014.7
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Hammerhead ribozyme activity and oligonucleotide duplex stability in mixed solutions of water and organic compounds Reviewed
S. Nakano, Y. Kitagawa, D. Miyoshi, N. Sugimoto
FEBS Open Bio 4 643 - 650 2014.7
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Drastic stabilization of parallel DNA hybridizations by a polylysine comb-type copolymer with hydrophilic graft chain Reviewed
D. Miyoshi, Y. Ueda, N. Shimada, S. Nakano, N. Sugimoto, and A. Maruyama
ChemMedChem 9 ( 9 ) 2156 - 2163 2014.7
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A Designed Small Protein For Controlling Site-Specific Mineralization Of Silica And Calcium On Dnas Reviewed
Kenji Usui, Kazuma Nagai, Hiroto Nishiyama, Aoi Yamada, Makoto Ozaki, Takaaki Tsuruoka, Kin-ya Tomizaki
PROTEIN SCIENCE 23 143 - 143 2014.7
Joint Work
Publisher：WILEY-BLACKWELL
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Design and evaluation of locked nucleic acid-based splice-switching oligonucleotides in vitro Reviewed
T. Shimo, K. Tachibana, K. Saito, T. Yoshida, E. Tomita, R. Waki, T. Yamamoto, T. Doi, T. Inoue, J. Kawakami and S. Obika
Nucleic Acids Res. 42 8174 - 8187 2014.6
Joint Work
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A nanocomposite approach to develop biodegradable thermogels exhibiting excellent cell-compatibility for injectable cell delivery Reviewed
N. Oyama, H. Minami, D. Kawano, M. Miyazaki, T. Maeda, K. Toma, A. Hotta, K. Nagahama
Biomaterials Science 2 ( 8 ) 1057 - 1062 2014.6
Joint Work
Authorship：Lead author
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Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers.
Higuchi S, Watanabe TM, Kawauchi K, Ichimura T, Fujita H
Journal of bioscience and bioengineering 117 ( 6 ) 749 - 755 2014.6
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Refinement and verification of test conditions for colony-forming unit assay in rat bone marrow cells with preservable colony specimens Reviewed
Takamatsu K., Wakata A., Tabata H., and Nishikata T.
Comparative Clinical Pathology 23 ( 3 ) 569 - 575 2014.5
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In vitro assays predictive of telomerase inhibitory effect of G-quadruplex ligands in cell nuclei Reviewed
H. Yaku, T. Murashima, D. Miyoshi, N. Sugimoto
The Journal of Physical Chemistry B 118 ( 10 ) 2605 - 2614 2014.3
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Effects of molecular crowding on the structures, interactions, and functions of nucleic acids Reviewed
S. Nakano, D. Miyoshi, and N. Sugimoto
Chem. Rev. 114 2733 - 2758 2014.3
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Site-Specific Control of Silica Mineralization on DNA Using Designed Peptides Reviewed
K. Usui, K. Nagai, H. Nishiyama, A. yamada, T. Tsuruoka, S. Fujii, K.-y. Tomizaki
Peptide Science 2013 2415 - 2416 2014.3
Joint Work
Authorship：Lead author
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A simple "add and measure" FRET-based telomeric tandem repeat sequence detection and telomerase assay method Reviewed
K. Kawamura, H. Yaku, D. Miyoshi, T. Murashima
Organic & Biomolecular Chemistry 12 ( 6 ) 936 - 941 2014.2
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Peptides targeting G-quadruplex structures Reviewed
Kenji Usui, Arisa Okada
Chemical Biology of Nucleic Acids: Fundamentals and Clinical Applications 459 - 475 2014.1
Publisher：Springer Berlin Heidelberg
Research in recent decades has revealed that some DNA and RNA secondary structures modulate a variety of cellular events. One secondary structure, the Guanine(G)-quadruplex, also regulates various cellular events that are mostly related to serious diseases. Systems capable of controlling DNA and RNA G-quadruplex structures would therefore be useful for the modulation of various cellular events to produce biological effects. Because of their biological importance, many G-quadruplex-targeting compounds have been described. However, the next generation of targeting molecules should exhibit increased G-quadruplex sequence specificity, a higher structure-inducing or -collapsing ability, and a greater degree of functionality, including on–off switches of binding ability and cellular penetration. Peptides might be good candidates for these next-generation Gquadruplex- targeting molecules due to the following advantages: (1) their easy design and synthesis, (2) their ability to mimic protein–G–quadruplex interactions, (3) the possibility of employing artificial amino acids in addition to naturally occurring amino acids, and (4) the ability to combine G-quadruplex-binding sequences with other functional sequences. Accordingly, several peptide-based compounds, such as furan-based cyclic peptides, PNA-conjugated peptides, and small molecule-peptide conjugates, have been developed. In this chapter, we introduce all these peptide ligands and describe most of the approaches for targeting G-quadruplex structures. We then conclude that peptides are among the most promising functional ligands for G-quadruplexes to control various biological events in next-generation approaches.
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Mechanistic study on the facilitation of enzymatic hydrolysis by α-glucosidase in the presence of betaine-type metabolite analogs Reviewed
Yuichi Nakagawa, Shiro Sehata, Satoshi Fujii, Hiroaki Yamamoto, Akihiko Tsuda, Kazuya Koumoto
Tetrahedron 70 5895 - 5903 2014
Joint Work
Authorship：Lead author
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Reduced Graphene Oxide Upconversion Nanoparticle Hybrid for Electrochemiluminescent Sensing of a Prognostic Indicator in Early-Stage Cancer Reviewed
Li Wu, Jiasi Wang, Meili Yin, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, Xiaogang Qu
Small 10 330 - 336 2014
Joint Work
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A fluorescent probe for detection of an intracellular prognostic indicator in early-stage cancer (10.1039/c4cc08789e Reviewed
Haiwei Ji, Yijia Guan, L Wu, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto Xiaogang Qu
Chem. Commun. 51 1479 - 1482 2014
Joint Work
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Preparation of hydrogels for the study of the effects of spatial confinement on DNA Reviewed
Shu-ichi Nakano, Masatoshi Yoshida, Daisuke Yamaguchi, Naoki Sugimoto
Transact. Mat. Res. Soc. Japan 39 435 - 438 2014
Joint Work
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Dangling ends perturb the stability of RNA duplex responsive to surrounding condition Invited Reviewed
H. Tateishi-Karimata, S. Pramanik, S. Nakano, D. Miyoshi and N. Sugimoto
ChemMedChem 9 ( 9 ) 2150 - 2155 2014
Joint Work
-
Hammerhead ribozyme activity and oligonucleotide duplex stability in mixed solutions of water and organic compounds Reviewed
Shu-ichi Nakano, Yuichi Kitagawa, Daisuke Miyoshi, Naoki Sugimoto
FEBS Open Bio 4 643 - 650 2014
Joint Work
-
Drastic stabilization of parallel DNA hybridizations by a polylysine comb-type copolymer with hydrophilic graft chain Reviewed
Daisuke Miyoshi, Yu-mi Ueda, Naohiko Shimada, Shu-ichi Nakano, Naoki Sugimoto, Atsushi Maruyama
ChemMedChem 9 ( 9 ) 2156 - 2163 2014
Joint Work
Authorship：Lead author
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Biosensors Based on Graphene and Biomolecules Invited Reviewed
Bioscience and Bioindustry 72 ( 2 ) 117 - 120 2014
Joint Work
Authorship：Lead author
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measure” FRET-based telomeric tandem repeat sequence detection and telomerase assay method Reviewed
Koji Kawamura, Hidenobu Yaku, Daisuke Miyoshi, Takashi Murashima
Org. Biomol. Chem. 12 934 - 941 2014
Joint Work
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Effects of Molecular Crowding on the Structures, Interactions, and Functions of Nucleic Acids Reviewed
Shu-ichi Nakano, Daisuke Miyoshi, Naoki Sugimoto
Chem. Rev. 114 ( 5 ) 2733 - 2758 2014
Joint Work
Authorship：Lead author
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In Vitro Assays Predicting of Telomerase Inhibitory Effect of G-Quadruplex Ligands in Cell Nuclei Reviewed
Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, Naoki Sugimoto
J. Phys. Chem. B 118 2605 - 2614 2014
Joint Work
Authorship：Lead author
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Preparation of hydrogels for the study of the effects of spatial confinement on DNA
Shu-ichi Nakano, Masatoshi Yoshida, Daisuke Yamaguchi, Naoki Sugimoto
Transact. Mat. Res. Soc. Japan 39 435 - 438 2014
Joint Work
-
Biosensors Based on Graphene and Biomolecules
Bioscience and Bioindustry 72 ( 2 ) 117 - 120 2014
Joint Work
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Synthesis and Chromatographic Evaluation of PFOS-selective Polymer by Molecular Imprinting
Rei Sakamoto, Keito Yuasa, Jun Matsui
Chromatography 35 ( Supplement1 ) 81 - 82 2014
Joint Work
Authorship：Lead author
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In Situ Synthesis of Metal/Polymer Nanocomposite Thin Films on Glass Substrates by using Highly Cross-Linked Polymer Matrices with Tailorable Ion Exchange Capabilities
Isao Toda, Takaaki Tsuruoka, Jun Matsui, Takashi Murashima, Hidemi Nawafune, Kensuke Akamatsu
RSC Advances 4 4723 - 4726 2014
Joint Work
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Synthesis and Chromatographic Evaluation of Affinity Polymer Having Zinc Porphyrin Moieties
Kota Matsuo, Yuki Minetoki, Jun Matsui
Chromatography 35 ( Supplement1 ) 79 - 80 2014
Joint Work
Authorship：Lead author
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Mitochondrial inhibitor sodium azide inhibits the reorganization of mitochondria-rich cytoplasm and the establishment of the anteroposterior axis in ascidian embryo Reviewed
Ishii H., Shirai T., Makino C. and Nishikata T
Devl. Growth Differ. 56 175 - 188 2014
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A Novel Assay System for Macrophage Activating Factor Activity Using a Human U937 Cell Line. Reviewed
Ishikawa M, Inoue T, Inui T, Kuchiike D, Kubo K, Uto Y, and Nishikata T,
Anticancer Research 34 ( 8 ) 4577 - 4581 2014
Joint Work
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Simultaneous Expression of Cancer Stem Cell-like Properties and Cancer-associated Fibroblast-like Properties in a Primary Culture of Breast Cancer Cells Reviewed
Ishikawa M, Inoue T, Shirai T, Takamatsu K, Kunihiro S, Ishii H, and Nishikata T
Cancers 6 1570 - 1578 2014
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A Facile Template Synthesis of Asymmetric Gold Silica Heteronanoparticles Reviewed
R. Miyanohata, T. Matsushita, T. Tsuruoka, H. Nawafune, K. Akamatsu
J. Colloid Interface Sci 416 147 - 150 2014
Joint Work
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Electroless nickel plating on polymer particles Reviewed
S. Fujii, H. Hamasaki, H. Takeoka, T. Tsuruoka, K. Akamatsu, Y. Nakamura
J. Colloid Interface Sci 430 47 - 55 2014
Joint Work
-
Direct fabrication of metal patterns on resin substrate by combining imprint and electrochemical lithography Reviewed
Y. Kimura, R. Fujiwara, T. Tsuruoka, Y. Takashima, H. Nawafune, H. Yanagimoto, K. Akamatsu
Journal of The Surface Finishing Society of Japan 66 18 - 19 2014
Joint Work
-
Electroless Deposition of Tin Using Contact with Zinc Powder in the Bath Reviewed
E. Uchida, K. Tanaka, A. Kawabata, S. Nabeshima, T. Tsuruoka, K. Akamatsu, H. Nawafune
Journal of The Surface Finishing Society of Japan 65 453 - 457 2014
Joint Work
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Fluorescence Enhancement of Immobilized CdTe Nanocrystals on Metal Nanocrystals-Modified Substrate by Dipole Coupling Reviewed
T. Tsuruoka, M. Yamashita, M. Kumano, K. Uwajima, H. Nawafune, K. Akamatsu
Trans. Mater. Res. Soc. Jpn. 39 481 - 484 2014
Joint Work
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Gold nanoparticle satellite nanostructures on glass substrate for dual-responsive optical sensing of organic liquids Reviewed
Y. Tanaka, A. Matsumoto, T. Tsuruoka, H. Nawafune, K. Akamatsu
Chem. Lett 43 1084 - 1085 2014
Joint Work
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Electroless nickel plating on polymer particles Reviewed
S. Fujii, H. Hamasaki, H. Takeoka, T. Tsuruoka, K. Akamatsu, Y. Nakamura
J. Colloid Interface Sci 430 47 - 55 2014
Joint Work
-
Direct fabrication of metal patterns on resin substrate by combining imprint and electrochemical lithography Reviewed
Y. Kimura, R. Fujiwara, T. Tsuruoka, Y. Takashima, H. Nawafune, H. Yanagimoto, K. Akamatsu
Journal of The Surface Finishing Society of Japan 66 18 - 19 2014
Joint Work
-
Electroless Deposition of Tin Using Contact with Zinc Powder in the Bath Reviewed
E. Uchida, K. Tanaka, A. Kawabata, S. Nabeshima, T. Tsuruoka, K. Akamatsu, H. Nawafune
Journal of The Surface Finishing Society of Japan 65 453 - 457 2014
Joint Work
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Fluorescence Enhancement of Immobilized CdTe Nanocrystals on Metal Nanocrystals-Modified Substrate by Dipole Coupling Reviewed
T. Tsuruoka, M. Yamashita, M. Kumano, K. Uwajima, H. Nawafune, K. Akamatsu
Trans. Mater. Res. Soc. Jpn. 39 481 - 484 2014
Joint Work
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Gold nanoparticle satellite nanostructures on glass substrate for dual-responsive optical sensing of organic liquids Reviewed
Y. Tanaka, A. Matsumoto, T. Tsuruoka, H. Nawafune, K. Akamatsu
Chem. Lett 43 1084 - 1085 2014
Joint Work
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Controlled Synthesis of Metal-Organic Framework Films on Metal Nanoparticles by the Versatile Layer-by-Layer Assembly Approach Reviewed
T. Ohhashi, T. Tsuruoka, H. Nawafune, K. Akamatsu
Trans. Mater. Res. Soc. Jpn 39 153 - 156 2014
Joint Work
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All‐Inorganic Water-Dispersible Silicon Quantum Dots: Highly Efficient Near-Infrared Luminescence in a Wide pH range
H. Sugimoto, M. Fujii, Y. Fukuda, K. Imakita, K. Akamatsu
Nanoscale 6 122 - 126 2014
Joint Work
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Electroless Bismuth Plating as a Peripheral Technology for SiC Power Devices Reviewed
E. Uchida, K. Tanaka, M. Okada, T. Tsuruoka, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn 39 47 - 51 2014
Joint Work
-
In Situ Synthesis of Metal/Polymer Nanocomposite Thin Films on Glass Substrates by using Highly Cross-Linked Polymer Matrices with Tailorable ion exchange capabilities Reviewed
I. Toda, T. Tsuruoka, J. Matsui, T. Murashima, H. Nawafune, K. Akamatsu
RSC Advances 4 4723 - 4726 2014
Joint Work
-
A Facile Template Synthesis of Asymmetric Gold Silica Heteronanoparticles
R. Miyanohata, T. Matsushita, T. Tsuruoka, H. Nawafune, K. Akamatsu
J. Colloid Interface Sci 416 147 - 150 2014
Joint Work
-
In situ Synthesis of Metal/Polymer Nanocomposite Thin Films on Glass Substrates by using Highly Cross-linked Polymer Matrices with Tailorable Ion Exchange Capabilities
I. Toda, T. Tsuruoka, J. Matsui, T. Murashima, H. Nawafune, K. Akamatsu
RSC Advances 3 16243 - 16246 2014
Joint Work
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Gold nanoparticle satellite nanostructures on glass substrate for dual-responsive optical sensing of organic liquids
Y. Tanaka, A. Matsumoto, T. Tsuruoka, H. Nawafune, K. Akamatsu
Chem. Lett 43 1084 - 1085 2014
Joint Work
-
Fluorescence Enhancement of Immobilized CdTe Nanocrystals on Metal-Nanocrystals-Modified Substrate by Dipole Coupling
T. Tsuruoka, M. Yamashita, M. Kumano, K, Uwajima, Nawafune, K. Akamatsu
Trans. Mater. Res. Soc. Jpn 39 481 - 484 2014
Joint Work
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Electroless Bismuth Plating as a Peripheral Technology for SiC Power Devices
E. Uchida, K. Tanaka, M. Okada, T. Tsuruoka, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn 39 47 - 51 2014
Joint Work
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Controlled Synthesis of Metal-Organic Framework Films on Metal Nanoparticles by the Versatile Layer-by-Layer Assembly Approach
T. Ohhashi, T. Tsuruoka, H. Nawafune, K. Akamatsu
Trans. Mater. Res. Soc. Jpn 39 153 - 156 2014
Joint Work
-
All-Inorganic Water-Dispersible Silicon Quantum Dots: Highly Efficient Near-Infrared Luminescence in a Wide pH range
H. Sugimoto, M. Fujii, Y. Fukuda, K. Imakita, K. Akamatsu
Nanoscale 6 122 - 126 2014
Joint Work
-
Thermodynamic Features Provided by an ENA Introduction to Nucleic Acid Drugs
E. Tomita, M. Sumimoto, J. Kawakami
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 188 - 189 2014
Joint Work
-
Mechanistic study on the facilitation of enzymatic hydrolysis by alpha-glucosidase in the presence of betaine-type metabolic analogs Reviewed
Yuichi Nakagawa, Shiro Sehata, Satoshi Fujii, Hiroaki Yamamoto, Akihiko Tsuda, Kazuya Koumoto
Tetrahedron 70 5895 - 5903 2014
Joint Work
-
Site-Specific Control of Silica Mineralization on DNA Using Designed Peptides Reviewed
K. Usui, K. Nagai, H. Nishiyama, A. Yamada, T. Tsuruoka, S. Fujii, K-y. Tomizaki
Peptide Science 2415 - 2416 2014
Joint Work
-
Electroless nickel plating on polymer particles Reviewed
S. Fujii, H. Hamasaki, H. Takeoka, T. Tsuruoka, K. Akamatsu, Y. Nakamura
J. Colloid Interface Sci. 430 47 - 55 2014
Joint Work
-
Direct fabrication of metal patterns on resin substrate by combining imprint and electrochemical lithography Reviewed
Y. Kimura, R. Fujiwara, T. Tsuruoka, Y. Takashima, H. Nawafune, H. Yanagimoto, K. Akamatsu
Journal of The Surface Finishing Society of Japan 66 18 - 19 2014
Joint Work
-
Electroless Deposition of Tin Using Contact with Zinc Powder in the Bath Reviewed
E. Uchida, K. Tanaka, A. Kawabata, S. Nabeshima, T. Tsuruoka, K. Akamatsu, H. Nawafune
Journal of The Surface Finishing Society of Japan 65 453 - 457 2014
Joint Work
-
Fluorescence Enhancement of Immobilized CdTe Nanocrystals on Metal Nanocrystals-Modified Substrate by Dipole Coupling Reviewed
T. Tsuruoka, M. Yamashita, M. Kumano, K. Uwajima, H. Nawafune, K. Akamatsu
Trans. Mater. Res. Soc.Jpn 39 481 - 484 2014
Joint Work
-
Gold nanoparticle satellite nanostructures on glass substrate for dual-responsive optical sensing of organic liquids Reviewed
Y. Tanaka, A. Matsumoto, T. Tsuruoka, H. Nawafune, K. Akamatsu
Chem. Lett 43 1084 - 1085 2014
Joint Work
-
Controlled Synthesis of Metal-Organic Framework Films on Metal Nanoparticles by the Versatile Layer-by-Layer Assembly Approach Reviewed
T.Ohhashi, T. Tsuruoka, H. Nawafune, K. Akamatsu
Trans. Mater. Res. Soc.Jpn 39 153 - 156 2014
Joint Work
-
Electroless Bismuth Plating as a Peripheral Technology for SiC Power Devices Reviewed
E. Uchida, K. Tanaka, M. Okada, T. Tsuruoka, K. Akamatsu H. Nawafune
Trans. Mater. Res. Soc.Jpn 39 47 - 51 2014
Joint Work
-
In Situ Synthesis of Metal/Polymer Nanocomposite Thin Films on Glass Substrates by using Highly Cross-Linked Polymer Matrices with Tailorable ion exchange capabilities Reviewed
I.Toda, T.Tsuruoka, J. Matsui, T. Murashima, H. Nawafune, K. Akamatsu
RSC Adovances 4 4723 - 4726 2014
Joint Work
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A Facile Template Synthesis of Asymmetric Gold Silica Heteronanoparticles Reviewed
R. Miyanohata, T. Mstsushita, T. Tsuruoka, H. Nawafune, K. Akamatsu
J. Colloid Interface Sci. 416 147 - 150 2014
Joint Work
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Towards Imaging Electron Density inside Metal-Organic Framework Structures Reviewed
Yohei Takashima, De-Liang Long, Leroy Cronin
Chem. Commun 50 2271 - 2274 2014
Joint Work
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Enhanced Phosphorescence Emission by Incorporating Aromatic Halides into an Entangled Coordination Framework Based on Naphthalenediimide Reviewed
Virginia Mart?nez-Mart?nez, Rebeca Sola Llano, Shuhei Furukawa, Yohei Takashima, I?igo L?pez Arbeloa, Susumu Kitagawa
ChemPhysChem 15 2517 - 2521 2014
Joint Work
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In situ synthesis of metal/polymer nanocomposite thin films on glass substrates by using highly cross-linked polymer matrices with tailorable ion exchange capabilities Reviewed
I. Toda, T. Tsuruoka, J. Matsui, T. Murashima, H. Nawafune, K. Akamatsu
RSC Advances 4 4723 - 4726 2013.12
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Control of Site-Specific Silica Precipitation Using PNA Peptides and DNAs Reviewed
K. Usui, H. Nishiyama, K. Nagai, T. Tsuruoka, S. Fujii, K.-y. Tomizaki
Peptides 2013 162 - 163 2013.12
Joint Work
Authorship：Lead author
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Designed Peptide Libraries for Cell Analyzing Microarrays Reviewed
H. Mihara, K. Usui, H. Tsutsumi, K. Nokihara
Peptides 2013 22 - 23 2013.12
Joint Work
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Peptide-DNA Hybrid G-Quadruplex Structures for Regulation of Protein Expression Depending on Protease Activity Reviewed
A. Okada, M. Taniguchi, K. Usui, N. Sugimoto
Peptides 2013 180 - 181 2013.12
Joint Work
Authorship：Lead author
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Cytoplasmic translocation of the retinoblastoma protein disrupts sarcomeric organization.
Araki K, Kawauchi K, Hirata H, Yamamoto M, Taya Y
eLife 2 e01228 2013.12
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Positional Effect of 2',4'-BNA/LNA Introduction into DNA/RNA Duplexes on Thermodynamic Parameters
S. Tahara, E. Tomita, H. Nagai, S. Obika, and J. Kawakami
Proc. 40th Intl. Symp. Nucleic Acid Chemistry 190 - 191 2013.11
Joint Work
Authorship：Lead author
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A highly sensitive telomerase activity assay that eliminates false-negative results caused by PCR inhibitors Reviewed
H. Yaku, T. Murashima, D. Miyoshi, N. Sugimoto
Molecules 18 ( 10 ) 11751 - 11767 2013.9
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Multiple and cooperative binding of fluorescence light-up probe thioflavin t with human telomere DNA G-quadruplex Reviewed
V. Gabelica, R. Maeda, T. Fujimoto, H. Yaku, T. Murashima, N. Sugimoto, D. Miyoshi
Biochemistry 52 ( 33 ) 5620 - 5628 2013.7
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Conformational changes of the phenyl and naphthyl isocyanate-DNA adducts during DNA replication and by minor groove binding molecules Reviewed
S. Nakano, Y. Uotani, Y. Sato, H. Oka, M. Fujii, and N. Sugimoto
Nucleic Acids Res. 41 8581 - 8590 2013.7
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In situ template synthesis of one-dimensional gold nanoparticle arrays in organic nanowires Reviewed
T. Matsushita, Y. Fukumoto, T. Kawakami, T. Tsuruoka, T. Murashima, T. Yanagishita, H. Masuda, H. Nawafune, K. Akamatsu
RSC Advances 3 16243 - 16246 2013.7
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Quantitative analyses of nucleic Acid stability under the molecular crowding condition induced by cosolutes Invited Reviewed
Tateishi-Karimata H, Nakano S, Sugimoto N.
Curr Protoc Nucleic Acid Chem. Chapter 7 Unit7.19 2013.6
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Systematic screening of the cellular uptake of designed alpha-helix peptides Reviewed
Kenji Usui, Takuya Kikuchi, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
BIOORGANIC & MEDICINAL CHEMISTRY 21 ( 9 ) 2560 - 2567 2013.5
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
The cellular penetration (CP) activity of functional molecules has attracted significant attention as one of the most promising new approaches for drug delivery. In particular, cell-penetrating peptides (CPPs) have been studied extensively in cellular engineering. Because there have been few large-scale systematic studies to identify peptide sequences with optimal CP activity or that are suitable for further applications in cell engineering, such as cell-specific penetration and cell-selective culture, we screened and compared the cellular uptake (CU) activity of 54 systematically designed a-helical peptides in HeLa cells. Furthermore, the CU activity of 24 designed peptides was examined in four cell lines using a cell fingerprinting technique and statistical approaches. The CU activities in various cells depended on amino acid residues of peptide sequences as well as charge, a-helical content and hydrophobicity of the peptides. Notably, the mutation of a single residue significantly altered the CU ability of a peptide, highlighting the variability of cell uptake mechanisms. Moreover, these results demonstrated the feasibility of cell-selective culture by conducting cell-selective permeation and death in cultures containing two cell types. These studies may lead to further peptide library design and screening for new classes of CPPs with useful functions. (C) 2013 Elsevier Ltd. All rights reserved.
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A peptide release system using a photo-cleavable linker in a cell array format for cell-toxicity analysis Invited Reviewed
Takashi Kakiyama, Kenji Usui, Kin-ya Tomizaki, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
POLYMER JOURNAL 45 ( 5 ) 525 - 539 2013.5
Joint Work
Authorship：Lead author Publisher：NATURE PUBLISHING GROUP
We constructed a novel peptide-array format system for cellular toxicity analysis. In this system, a peptide was immobilized on a conventional 96-well plate bottom via a photo-cleavable linker. Once UV light irradiated the desired wells, the peptide was released from the bottom. As a result, the cytotoxic behavior of the peptide could be monitored. Immobilization and light-irradiation conditions were optimized. The immobilized peptide showed no cytotoxicity; therefore, the cells could be cultured on the peptide-immobilizing plate from the beginning of the experiment. Cell-toxicity assays with this system for three cell types were performed. All cell types showed similar to 25% lowering of viability with the photo-released 5-(and-6)-carboxytetramethylrhodamine (TMR)-GKLAKLAKKLAKLAKKLAKLAKGC (TMR-KLA-C) peptide compared with the non-coated plate. This relative toxicity nearly corresponded to that of similar to 10 mu M TMR-KLA-C in solution, and we found that the released peptide concentration per well was similar to 10 mu M at 60 min irradiation. Throughout this study, we successfully immobilized peptides via the photo-cleavable linker, released them by UV irradiation spatiotemporally and conducted the cell-toxicity assay. This study implies that the peptide photo-releasing array system will allow the realization of high-throughput cell arrays for cellomics analyses and cell-based phenotypic drug screenings.
DOI： 10.1038/pj.2013.20
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Biodegradable polymers exhibiting temperature-responsive sol-gel transition as injectable biomedical materials Reviewed
Koji Nagahama, Akihiro Takahashi, Yuichi Ohya
Reactive and Functional Polymers ( 73 ) 979 - 985 2013.5
Joint Work
Authorship：Lead author
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Study on effects of molecular crowding on G-quadruplex-ligand binding and ligand-mediated telomerase inhibition Reviewed
H. Yaku, T. Murashima, H. Tateishi-Karimata, S. –I. Nakano, D. Miyoshi, N. Sugimoto
Methods 64 ( 1 ) 19 - 27 2013.4
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A PNA Peptide for the Control of Site-Specific Silica Precipitation on DNA Reviewed
Kenji Usui, Kazuma Nagai, Hiroto Nishiyama, Takaaki Tsuruoka, Satoshi Fujii
Peptide Science 2012 375 - 376 2013.3
Joint Work
Authorship：Lead author
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Construction of Artificial Peptides for Control of DNA G-quadruplex Structures Depending on Protease Activity toward Regulation of Protein Expression Reviewed
Arisa Okada, Kenji Usui, Mai Taniguchi, Keita Kobayashi, Naoki Sugimoto
Peptide Science 2012 285 - 286 2013.3
Joint Work
Authorship：Lead author
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Enhanced stereocomplex formation of enantiomeric polylactides grafted on a polyrotaxane platform Reviewed
Koji Nagahama, Rie Aoki, Toshifumi Saito, Tatsuro Ouchi, Yuichi Ohya and Nobuhiko Yui
Polymer Chemistry ( 4 ) 1769 - 1773 2013.3
Joint Work
Authorship：Lead author
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Design of Biodegradable Injectable Polymers Exhibiting Temperature-Responsive Sol-Gel Transition Reviewed
Y. Ohya, H. Suzuki, K. Nagahama, A. Takahashi, T. Ouchi, A. Kuzuya
Advances in Science and Technology ( 86 ) 9 - 16 2013.3
Joint Work
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Thermodynamics - Hydration Relationships within Loops that Affect G-Quadruplexes under Molecular Crowding Conditions Reviewed
Takeshi Fujimoto, Shu-ichi Nakano, Naoki Sugimoto, Daisuke Miyoshi
J. Phys. Chem. B 117 963 - 972 2013.1
Joint Work
Authorship：Lead author
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Thermodynamics-hydration relationships within loops that affect G-quadruplexes under molecular crowding conditions Reviewed
T. Fujimoto, S. Nakano, N. Sugimoto, and D. Miyoshi
J. Phys. Chem. B 117 ( 4 ) 963 - 972 2013.1
Joint Work
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Biochemical purification of mitochondrial HSP60 and ATPsynthase from ascidian eggs: Implications for antibody specificity Reviewed
Janet Chenevert, Gerard Pruliere, Hirokazu Ishii, Christian Sardet, Takahito Nishikata
PLoS One 8 ( 1 ) e52996 2013.1
Joint Work
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Multiple and cooperative binding of fluorescence light-up probe Thioflavin T with human telomere DNA G-quadruplex Reviewed
Valerie Gabelica, Ryuichi Maeda, Takeshi Fujimoto, Hidenobu Yaku, Takashi Murashima, Naoki Sugimoto, and Daisuke Miyoshi
Biochemistry 52 5620 - 5628 2013
Joint Work
Authorship：Lead author
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A Highly Sensitive Telomerase Activity Assay that Eliminates False-Negative Results Caused by PCR Inhibitors Reviewed
Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, and Naoki Sugimoto
Molecules 18 ( 10 ) 11751 - 11767 2013
Joint Work
Authorship：Lead author
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Sequence and Solvent Effects on Telomeric DNA Bimolecular G-Quadruplex Folding Kinetics Reviewed
Adrien Marchand, Ruben Ferreira, Hisae Tateishi-Karimata, Daisuke Miyoshi, Naoki Sugimoto, Valerie Gabelica
J. Phys. Chem. B 117 ( 41 ) 12391 - 12401 2013
Joint Work
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Recognition of Perfluorooctanoic Acid (PFOA) by Fluorous Molecularly Imprinted Polymer
34 ( Supplement1 ) 21 - 22 2013
Joint Work
Authorship：Lead author
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Application of a Reflectometric Interference Spectroscopic Device (RIfS) with a Polymer-gel Coated SiN Chip to an HPLC Detector
Yuma Nakahori, Yusuke Sugahara, Jun Matsui
Chromatography 34 ( Supplement1 ) 51 - 52 2013
Joint Work
Authorship：Lead author
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Primary culture of breast cancer: A model system for epithelial-mesenchymal transition and cancer stem cells Reviewed
Nishikata T., Ishikawa M., Matsuyama T., Takamatsu K., Fukuhara T., and Konishi Y.
Anticancer Research 33 2867 - 2874 2013
Joint Work
Authorship：Lead author
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Purification of mitochondrial proteins HSP60 and ATP synthase from ascidian eggs: implications for antibody specificity. Reviewed
Chenevert J, Pruliere G, Ishii H, Sardet C, Nishikata T
PloS one 8 ( 1 ) e52996 2013
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Phosphorus and Boron Codoped Colloidal Silicon Nanocrystals with Inorganic Atomic Ligandas Reviewed
H.Sugimoto, M.Fujii, K.Imakita, S.Hayashi, K.Akamatsu
J.Phys Chem.C. 117 6807 - 6813 2013
Joint Work
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Synthesis of Polystyrene-based Nanocomposite Thin Films with Domain Structure Consisting of Au Nanoparticles Reviewed
T. Tsuruoka, S. Kumazaki, I. Osaka, H. Nawafune, K. Akamatsu
Journal of Physics: Conference Series 417 012020 - 012020 2013
Joint Work
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In Situ Template Synthesis of One-dimensional Gold Nanoparticle Arrays in Organic Nanowires Reviewed
T. Matsushita, Y. Fukumoto, T. Kawakami, T. Tsuruoka, T. Murashima, T. Yanagishita, H. Masuda, H. Nawafune, K. Akamatsu
RSC Advances 3 16243 - 16246 2013
Joint Work
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Codoping n- and p-type impurities in colloidal silicon nanocrystals:controlling luminescence energy from below bulk band gap to visible range
H. Sugimoto, M. Fujii, K. Imakita, S. Hayashi, K Akamatsu
J. Phys. Chem C.117(22) 11850 - 11857 2013
Joint Work
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Shape Memory Nanopores Induced in Coordination Frameworks by Crystal Downsizing
Y. Sakata, S. Furukawa, M. Kondo, K. Hirai, N. Horike, Y.Takashima, H. Uehara, N. Louvain, M. Meilikhov, T. Tsuruoka, S. Isoda, W. Kosaka, O. Sakata, S. Kitagawa
Science 339 193 - 196 2013
Joint Work
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Phosphorus and Boron Codoped Colloidal Silicon Nanocrystals with Inorganic Atomic Ligands
H. Sugimoto, M. Fujii, K. Imakita, S. Hayashi, K Akamatsu
J. Phys. Chem C.117(13) 6807 - 6813 2013
Joint Work
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In Situ Template Synthesis of One-dimensional Gold Nanoparticle Arrays in Organic Nanowires
T. Matsushita, Y. Fukumoto, T. Kawakami, T. Tsuruoka, T. Murashima, T. Yanagishita, H. Masuda, H. Nawafune, K. Akamatsu
RSC Advances 3 16243 - 16246 2013
Joint Work
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Specific recognition of cytosine by hypoxanthine in pyrrolidinyl peptide nucleic acid Reviewed
C. Vilaivan, W. Srinarang, N. Yotapan, W. Mansawat, C. Boonlua, J. Kawakami, Y. Yamaguchi, Y. Tanaka, and T. Vilaivan
Org. Biomol. Chem. 11 2310 - 2317 2013
Joint Work
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Control of Site-Specific Silica Precipitation Using PNA Peptides and DNAs Reviewed
Kenji Usui, Hiroto Nishiyama, Kazuma Nagai, Takaaki Tsuruoka, Satoshi Fujii, Kin-ya Tomizaki
Peptide Science 162 - 163 2013
Joint Work
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A novel array format for monitoring cellular uptake using a photo-cleavable linker for peptide release Reviewed
Kenji Usui, Takuya Kikuchi, Kin-ya Tomizaki, Takashi Kakiyama, Hisakazu Mihara
CHEMICAL COMMUNICATIONS 49 ( 57 ) 6394 - 6396 2013
Publisher：ROYAL SOC CHEMISTRY
We developed a novel peptide array format incorporating a photo-cleavable linker for monitoring cellular uptake. Model peptides were successfully immobilised via the photo-cleavable linker onto conventional plates and could be released spatiotemporally using UV irradiation. Incorporation of confocal microscopy allowed for detailed real-time monitoring of cellular internalisation of peptides.
DOI： 10.1039/c3cc41632a
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Shape Memory Nanopores Induced in Coordination Frameworks by Crystal Downsizing Reviewed
Y.Sakata, S. Fukumoto, M. Kondo, K. Hirai, N. Horike, Y. Takashima, H.Uehara, N. Louvain, M.Meilikhov, T.Tsuruoka, S.Isoda, W.Kosaka, O.Sakata, S.Kitagawa
Science 339 193 - 196 2013
Joint Work
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A PNA Peptide for the Control of Site-Specific Silica Precipitation on DNA Reviewed
K. Usui, K. Nagai, H. Nishiyama, T. Tsuruoka, Satoshi Fujii
Peptide Science 375 - 376 2013
Joint Work
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Control of Site-Specific Silica Precipitation Using PNA Peptides and DNAs Reviewed
K. Usui, H. Nishiyama, K. Nagai, T. Tsuruoka, S. Fujii K-y. Tomizaki
Peptide 162 - 162 2013
Joint Work
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Shape Memory Nanopores Induced in Coordination Frameworks by Crystal Downsizing Reviewed
Yoko Sakata, Shuhei Furukawa, Mio Kondo, Kenji Hirai, Nao Horike, Yohei Takashima, Hiromitsu Uehara, Nicolas Louvain, Mikhail Meilikhov, Takaaki Tsuruoka, Seiji Isoda, Wataru Kosaka, Osami Sakata, Susumu Kitagawa
Science 339 193 - 196 2013
Joint Work
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Base-pairing selectivity of a ureido-linked phenyl-2'-deoxycytidine derivative Reviewed
Nakano S, Oka H, Yamaguchi D, Fujii M, Sugimoto N.
Org Biomol Chem 10 ( 48 ) 9664 - 9670 2012.12
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The beads-on-a-string structure of long telomeric DNAs under molecular crowding conditions Reviewed
Haiqing Yu, Gu Xiaobo Gu, Shu-ichi Nakano, Daisuke Miyoshi, Naoki Sugimoto
J. Am. Chem. Soc. (Higthlighted as the cover and introduced in the Spotslights on Recent JACS Publications) 134 20060 - 20069 2012.12
Joint Work
Authorship：Lead author
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The beads-on-a-string structure of long telomeric DNAs under molecular crowding conditions Reviewed
Yu H, Gu X, Nakano S, Miyoshi D, Sugimoto N.
J Am Chem Soc. 134 ( 49 ) 20060 - 20069 2012.12
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Aptamer carbon nanodot sandwich used for fluorescent detection of protein Reviewed
Bailu Xu, Chuanqi Zhao, Weili Wei, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, and Xiaogang Qu
Analyst 137 5483 - 5486 2012.12
Joint Work
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Cytosolic subunits of ATP synthase are localized to the cortical endoplasmic reticulum-rich domain of the ascidian egg myoplasm. Reviewed
Hirokazu Ishii, Shiori Kunihiro, Manabu Tanaka, Kyoko Hatano and Takahito Nishikata
Devlopment, Growth and Differentiation 54 753 - 766 2012.12
Joint Work
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Construction of a trans-acting system of a L-histidine dependent ribozyme based on the secondary structure
S. Tanaka, Y. Yamaguchi, R. Takamine, and J. Kawakami
Proc. 39th Intl. Symp. Nucleic Acid Chemistry 234 - 235 2012.11
Joint Work
Authorship：Lead author
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Myosin II-dependent exclusion of CD45 from the site of Fcγ receptor activation during phagocytosis.
Yamauchi S, Kawauchi K, Sawada Y
FEBS letters 586 ( 19 ) 3229 - 3235 2012.9
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Mechanistic Study on Microstructural Tuning of Metal Nanoparticle/ Polymer Composite Thin Layers: Hydrogenation and Decomposition of Polyimide Matrices Catalyzed by Embedded Nickel Nanoparticles Reviewed
Kensuke Akamatsu , Maki Fujii , Takaaki Tsuruoka , Shu-ichi Nakano , Takashi Murashima , and Hidemi Nawafune
The Journal of Physical Chemistry C 116 ( 33 ) 17947 - 17954 2012.8
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Mechanistic study on microstructural tuning of metal nanoparticle/polymer composite thin layers: Hydrogenation and decomposition of polyimide matrices catalyzed by embedded nickel nanoparticles. Reviewed
K. Akamatsu, M. Fujii, T. Tsuruoka, S. Nakano, T. Murashima, and H. Nawafune
J. Phys. Chem. C 116 17947 - 17954 2012.8
Joint Work
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Specific Binding of Anionic Porphyrin and Phthalocyanine to the G-quadruplex Invited Reviewed
Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, Naoki
Molecules 17 10586 - 10613 2012.8
Joint Work
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p130Cas-dependent actin remodelling regulates myogenic differentiation.
Kawauchi K, Tan WW, Araki K, Abu Bakar FB, Kim M, Fujita H, Hirata H, Sawada Y
The Biochemical journal 445 ( 3 ) 323 - 332 2012.8
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Dimerization of nucleic acid hairpins in the conditions caused by neutral cosolutes Reviewed
S. Nakano, H. Hirayama, D. Miyoshi, and N. Sugimoto
J. Phys. Chem. B 116 ( 25 ) 7406 - 7415 2012.6
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Monodispersed Moleculary Imprinted Polymer Beads with Enhanced Atranzine Retention Ability Synthesized with Polymeric Diluents
Tsuyoshi Nagata, Shou Goji, Kensuke Akamatsu, Hidemi Nawafune, Jun Matsui
Analytical Letters 9 572 - 578 2012.6
Joint Work
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Colorimetric Detection of Perfluorooctanoic Acid (PFOA) Utilizing Polystyrene-Modified Gold Nanoparticles Reviewed
M. Takayose, K. Akamatsu, H. Nawafune, T. Murashima, J. Matsui
Analytical Letters 45 ( 18 ) 2856 - 2864 2012.5
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Hydration changes upon DNA folding studied by osmotic stress experiments Reviewed
S. Nakano, D. Yamaguchi, H. Tateishi-Karimata, D. Miyoshi, and N. Sugimoto
Biophys. J. 102 ( 12 ) 2808 - 2817 2012.5
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Biodegradable Stereocomplex Materials of Polylactide-grafted Dextran Exhibiting Soft and Tough Properties in Dry and Wet States Reviewed
K. Nagahama, K. Shimizu, S. Ichimura, A. Takahashi, T. Ouchi, Y. Ohya
Journal of Polymer Science Part A: Polymer Chemistry ( 50 ) 2669 - 2676 2012.5
Joint Work
Authorship：Lead author
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Biodegradable Polymeric Assemblies for Biomedical Materials Invited Reviewed
Y. Ohya, A. Takahashi, K. Nagahama
Advances in Polymer Science ( 247 ) 65 - 114 2012.4
Joint Work
Authorship：Lead author
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Screening of Designed Peptides for Binders to a DNA G-Quadruplex Structure Reviewed
N. Matsui, K. Kobayashi, K. Usui
Peptide Science 2011 2011 315 - 316 2012.3
Joint Work
Authorship：Lead author
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Development of PNA-Peptides Controlling DNA G-Quadruplex Structures Depending on Protease Activity Reviewed
K. Usui, K. Kobayashi, N. Sugimoto
Peptide Science 2011 2012 315 - 316 2012.3
Joint Work
Authorship：Lead author
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De Novo Designed Nitric Oxide Sensor Protein consisted of Iron Complex-Peptide Conjugate Reviewed
H. Miyazaki, K. Usui, S. Fujii
Peptide Science 2011 2011 303 - 304 2012.3
Joint Work
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Chromatin plasticity as a differentiation index during muscle differentiation of C2C12 myoblasts.
Watanabe TM, Higuchi S, Kawauchi K, Tsukasaki Y, Ichimura T, Fujita H
Biochemical and biophysical research communications 418 ( 4 ) 742 - 747 2012.2
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Detection of a Prognostic Indicator in Early-Stage Cancer Using Functionalized Graphene-Based Peptide Sensors Reviewed
Lingyan Feng, Li Wu, Jiasi Wang, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, Xiaogang Qu
Advanced Materials 24 ( 1 ) 125 - 131 2012.1
Joint Work
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Colorimetric Detection of Perfluorooctanoic Acid (PFOA) Utilizing Polystyrene-Modified Gold Nanoparticles Reviewed
Megumi Takayose, Kensuke Akamatsu, Hidemi Nawafune, Takashi Murashima, Jun Matsui
Analytical Letters 2012
Joint Work
Authorship：Lead author
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A fluorous synthetic receptor that recognizes perfluorooctanoic acid (PFOA) via fluorous interaction obtained by molecular imprinting Reviewed
Megumi Takayose, Kenji Nishimoto, Jun Matsui
Analyst 2012
Joint Work
Authorship：Lead author
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Monodispersed Molecularly Imprinted Polymer Beads with Enhanced Atrazine Retention Ability Synthesized with Polymeric Diluents Reviewed
Tsuyoshi Nagata, Shou Goji, Kensuke Akamatsu, Hidemi Nawafune, Jun Matsui
Analytical Letters 2012
Joint Work
Authorship：Lead author
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ポリイミド樹脂/金属間におけるナノ構造界面の構築と樹脂上への金属パターン形成
福本ユリナ、谷山智紀、鶴岡孝章、縄舟秀美、赤松謙祐
エレクトロニクス実装学会誌 15 534 - 540 2012
Joint Work
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Mechanistic Study on Microstructural Tuning of Metal Nanoparticle/Polymer Composite Thin Layers:Hydrogenation and Decomposition of Polyimide Matrices Ctalyzed By Embedded Nickel Nanoparticles
K. Akamatsu, M. Fujii, T. Tsuruoka, S. Nakano, T. Murashima, H. Nawafune
J. Phys. Chem. C 116 1794 - 1795 2012
Joint Work
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In situ synehesis of composite nanowires consisting of metal nanoparticles in ion-exchangeable polymer matrix using porous alumina tenplates Reviewed
Y.Fukumoto, T.Tsuruoka, T.Yangishita, H.Masuda, H.Nawafune, K. Akamatsu
Ttrans.Mater.Res.Soc.Jpn 37 185 - 188 2012
Joint Work
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Synthesis of Au Nanocrustals/Porous Coordination Polymer Composites by Preferential Self-Assembly of Frameworks Reviewed
H.Kawasaki, M.Kumano, M. Yamashita, T. Tsuruoka, H.Nawafune, K.Akamatsu
Trans. Mater. Res. Soc. Jpn 37 181 - 184 2012
Joint Work
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Enhancement of upconversion luminescence of AI2O3, Er3+thin films by small assemblies of Au nanorods Reviewed
T.Nakano, M. Fujii, K. Imakita, S. Hayashi, T.Tsuruoka, K. Akamatsu
Trans. Mater. Res. Soc. Jpn 38 449 - 452 2012
Joint Work
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Colorimetric Detection of Perfluorooctanoic Acid (PHOA) Utilizing Polystyrene-Modified Gold Nanoparticles
Megumi Takayose, Kensuke Akamatsu, Hidemi Nawafune, Takashi Murashima, Jun Matsui
Analytical Letters 18 2856 - 2864 2012
Joint Work
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All‐Inorganic Near-Infrared Luminescent Colloidal Silicon Nanocrystals:High Dispersibility in Polar liquid by Phosphorus and Boron Codoping
H. Sugimoto, M. Fujii, K. Imakita, S. Hayashi, K. Akamatsu
J. Phys. Chem. C 116 17969 - 17974 2012
Joint Work
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All-Inorganic Near-Infrared Luminescent Colloidal Silicon Nanocrystals:High Dispersibility in Polar Liquid by Phosphorus and Boron Codoping
Fujii, K. Imakita, S. Hayashi, K. Akamatsu
J. Phys. Chem C.116(33) 17969 - 17974 2012
Joint Work
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ポリイミド樹脂/金属間におけるナノ構造界面の構築と樹脂上への金属パターン形成
福本ユリナ、谷山智紀、鶴岡孝章、縄舟秀美、赤松謙祐
エレクトロニクス実装学会誌 15 534 - 540 2012
Single Work
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Synthesis of Polystyrene-based Nanocomposite Thin Films with Domain Structure Consisting of Au Nano
T. Tsuruoka, S. Kumazaki, I. Osaka, H. Nawafune, K. Akamatsu
Journal of Physics 2012
Joint Work
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Synthesis of Au Nanocrystals/Porous Coordination Polymer Composites by Preferential Self-Assembly of Frameworks
H. Kawasaki, M. Kumano, M. Yamashita, T. Tsuruoka, H. Nawafune, K. Akamatsu
Trans. Mater. Res. Soc. Jpn 37 181 - 184 2012
Joint Work
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Monodispersed Molecularly Imprinted Polymer Beads with Enhanced Atranzine Retention Ability Synthesized with Polymeric Diluents
Tsuyoshi Nagata, Shou Goji, Kensuke Akamatsu, Hidemi Nawafune, Jun Matsui
Anal. Lett 9 572 - 578 2012
Joint Work
-
Mechanistic Study on Microstructural Tuning of Metal Nanoparticle/Polymer Composite Thin Layers: Hydrogenation and Decomposition of Polyimide Matrices Ctalyzed By Embedded Nickel Nanoparticles
K. Akamatsu, M. Fujii, T. Tsuruoka, S. Nakano, T. Murashima, H. Nawafune
J. Phys. Chem C.116 1794 - 1795 2012
Joint Work
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In situ synthesis of composite nanowires consisting of metal nanoparticles in ion-exchangeable polymer matrix using porous alumina tenplates
Y. Fukumoto, T. Tsuruoka, T. Yangishita, H. Masuda, H. Nawafune, K. Akamatsu
Trans. Mater. Res. Soc. Jpn 37 185 - 188 2012
Joint Work
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Enhancement of upconversion Iuminescence of Al2O3 : Er3+, Yb3+ thin films by small assemblies of Au nanorods
T.Nakano, M.Fujii, K. Imakita, S. Hayashi, T. Tsuruoka, K. Akamatsu
Trans. Mater. Res. Soc. Jpn 38 449 - 452 2012
Joint Work
-
Colorimetric Detection of Perfluorooctanoic Acid (PFOA)Utilizing Polystyrene-Modified Gold Nanoparticles
Megumi Takayose, Kensuke Akamatsu, HIdemi Nawafune, Takashi Murashima, Jun Matsui
Anal. Lett 18 2856 - 2864 2012
Joint Work
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Phthalocyanines: A new class of G-quadruplex-ligands with many potential applications（共著） Reviewed
Yaku, H., Fujimoto, T., Murashima, T., Miyoshi, D., Sugimoto, N.
Chemical Communications 48 ( 50 ) 6203 - 6216 2012
Joint Work
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Specific binding of anionic porphyrin and phthalocyanine to the G-quadruplex with a variety of in Vitro and in Vivo applications Reviewed
H. Yaku, T. Murashima, D. Miyoshi, N. Sugimoto
Molecules 17 10586 - 10613 2012
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Mechanistic Study on Microstructural Tuning of Metal Nanoparticle/Polymer Composite Thin Layers:Hydrogenation and Decomposition of Polyimide Matrices Ctalyzed By Embedded Nickel Nanopoarticles Reviewed
K. Akamatsu, M. Fujii, T. Tsuruoka, S. Nakano, T. Murashima, H. Nawafune
J. Phys. Chem. C 2012
Joint Work
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Synthesis of Polystyrene-based Nanocomposite Thin Films with Domain Structure Consisting if Au Nanoparticles Reviewed
T. Tsuruoka, S. Kumazaki, I. Osaka, H. Nawafune, K.Akamatsu
J. Physics 2012
Joint Work
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ポリイミド樹脂/金属間におけるナノ構造界面の構造と樹脂上への金属パターン形成 Reviewed
福本ユリナ、谷山智紀、鶴岡孝章、縄舟秀美、赤松謙祐
エレクトロニクス実装学会誌 15 534 - 540 2012
Joint Work
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Charge Transfer and Exciplex Emissions from a Naphthalenediimide Entangled Coordination Framework accommodating Various Aromatic Guests Reviewed
Virginia Mart?nez-Mart?nez, Shuhei Furukawa, Yohei Takashima, I?igo L?pez Arbeloa, Susumu Kitagawa
J. Phys. Chem 116 26084 - 26090 2012
Joint Work
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DNA devises responding to cellular environmental factors Invited
D. Miyoshi
Chemistry and chemical industry (Chemical Society of Japan) 64 ( 12 ) 956 - 956 2011.12
Single Work
Authorship：Lead author
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Effects of protein binding on DNA G-quadruplex structures Invited
S. Nagatoishi, D. Miyoshi and N. Sugimoto
Int. Rev. Biophys. Chem. 2 129 - 134 2011.12
Joint Work
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Synthesis and Application of Functional Nucleic Acids Invited
Daisuke Miyoshi, Friedrich C. Simmel, Souvik Maiti, and Luis A. Marky
Journal of Nucleic Acids 2011 547245 2011.12
Joint Work
Authorship：Lead author
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DNA devises responding to cellular environmental factors
D. Miyoshi
Chemistry and chemical industry (Chemical Society of Japan) 64 ( 12 ) 956 - 956 2011.12
Single Work
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Effect of Nickeｌ(II) ion on Copper Electrodeposition from Ethylenediamine Complex Bath
H. Arimura, T. Shigematsu, T. Tsuruoka, K. Akamatsu, H. Nawafune
Journal of The Surface Finishing Society of Japan 62 708 - 711 2011.12
Joint Work
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Effect of Nickel(Ⅱ）ion on Copper Electrodeposition from Ethylenediamine Complex Bath
H. Arimura, T. Shigematsu, T. Tsuruoka, K. Akamatsu
Journal of The Surface Finishing Society of Japan 62 708 - 711 2011.12
Joint Work
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Rational design of a new IMP aptamer based on a TPP riboswitch and a hypoxanthine aptamer Reviewed
Misato Tokui, Takahiro Yamauchi, Daisuke Miyoshi, Hisaya Kamiya, Akira Nishimura, Naoki Sugimoto
Chemistry Letters 40 ( 11 ) 1313 - 1314 2011.11
Joint Work
Authorship：Lead author
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Measurements of the binding of a large protein using a substrate density-controlled DNA chip Reviewed
S. Nakano, T. Kanzaki, M. Nakano, D. Miyoshi, N. Sugimoto
Analytical Chemistry 83 6368 - 6372 2011.11
Joint Work
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Structure-function relationship of a histidine dependent ribozyme
Y. Yamaguchi, Y. Ishibashi, S. Tanaka, A. Tanigawa, I. Matsunaga, and J. Kawakami
Proc. 38th Intl. Symp. Nucleic Acids Chemistry 194 - 195 2011.11
Joint Work
Authorship：Lead author
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Cell fingerprint patterns using designed alpha-helical peptides to screen for cell-specific toxicity Reviewed
Kenji Usui, Takashi Kakiyama, Kin-ya Tomizaki, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 21 ( 21 ) 6281 - 6284 2011.11
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
We conducted cell-based cytotoxicity screening of a 101-membered alpha-helical peptide library using cell fingerprints (CFPs). The CFP data suggested that there is a relationship between cytotoxicity and peptide characteristics, such as hydrophobicity, charge, and amino acid composition. In spite of the small size of the library used in this study, several peptides demonstrated cell-specific toxicity. The strategy of combining a designed peptide library with CFP thus shows real promise for peptide-based screening with cells. (C) 2011 Elsevier Ltd. All rights reserved.
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Triplet analysis that identifies unpaired regions of functional RNAs（共著） Reviewed
J. Kawakami, Y. Yamaguchi, and N. Sugimoto
Journal of Nucleic Acids 2011 doi:10.4061/2011/471843 2011.10
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The effects of molecular crowding on the structure and stability of G-quadruplexes with an abasic site Invited Reviewed
T. Fujimoto, S. Nakano, D. Miyoshi, and N. Sugimoto
Journal of Nucleic Acids (Hindawl Publishing Corporation) 2011 ( 857149 ) 857149 2011.9
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A Novel Nitric Oxide Sensor Using Fluorescenct Peptides Attached to Iron Complexes
Hiroshi Miyazaki, Kenji Usui, Satoshi Fujii
Peptides: Building Bridges. Proceeding of the Twenty-Second American Peptide Symposium 224 - 225 2011.9
Joint Work
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Graphene biosensors Invited
D. Miyoshi, N. Sugimoto
Chemistry 66 ( 8 ) 30 - 35 2011.9
Joint Work
Authorship：Lead author
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The effects of molecular crowding on the structure and stability of G-quadruplexes with an abasic site Reviewed
Takeshi Fujimoto, Shu Ichi Nakano, Daisuke Miyoshi, and Naoki Sugimoto
J. Nucleic Acids 2011 857149 2011.9
Joint Work
Authorship：Lead author
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Graphene biosensors
D. Miyoshi, N. Sugimoto
Chemistry 66 ( 8 ) 30 - 35 2011.9
Joint Work
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Fabrication of Silver Patterns on Polyimide Films Based on Solid-Phase Electrochemical Constructive Lithography Using Ion-Exchangeable Precursor Layers
K. Akamatsu, Y. Fukumoto, T. Taniyama, T. Tsuruoka, H. Yanagimoto, H. Nawafune
Langmuir 27 11761 - 11766 2011.9
Joint Work
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Contlloed Self-Assembly of Metal-Organic Frameworks on Metal Nanoparticles for Efficient Synthesis of Hybrid Nanostructures
T. Tsuruoka, H. Kawasaki, H. Nawafune, K. Akamatsu
Contlloed Self-Assembly of Metal-Organic Frameworks on Metal Nanoparticles for Efficient Synthesis of Hybrid Nanostructures 3 3788 - 3791 2011.9
Joint Work
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Contlloed Self-Assembly of Metaｌ-Organic Frameworks on Metal Nanoparticle for Efficient Synthesis of Hybrid Nanostructures Reviewed
T.Tsuruoka, H.Kawasaki, H. Nawafune, K. Akamatsu
ACS Appl. Mater. Interfaces 3 3788 - 3791 2011.9
Joint Work
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Fabrication of Silver Patterns on Polyimide Films Based on Solid-Phase Electrochemical Constructive Lithography Using Ion-Exchangeable Precursor Layers
K. Akamatsu, Y. Fukumoto, T. Taniyama, T. Tsuruoka, H.Yanagimoto, H. Nawafune
Langmuir 27 11761 - 11766 2011.9
Joint Work
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Measurements of the binding of a large protein using a substrate density-controlled DNA chip Reviewed
S. Nakano, T. Kanzaki, M. Nakano, D. Miyoshi, and N. Sugimoto
Anal. Chem. 83 ( 16 ) 6768 - 6372 2011.8
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Effect of locked nucleic acid modifications on the thermal stability of noncanonical DNA structure Reviewed
J. Bhattacharyya, S. Maiti, S. Muhuri, S. Nakano, D. Miyoshi, and N. Sugimoto
Biochemistry 50 ( 34 ) 7414 - 7425 2011.8
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Label-free colorimetric and quantitative detection of cancer marker protein using noncrosslinking aggregation of Au/Ag nanoparticles induced by target-specific peptide probe Reviewed
Xiaohui Wang, Li Wu, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, and Xiaogang Qu
Biosensors and Bioelectronics 26 4804 - 4809 2011.8
Joint Work
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In situ generation of silver nanoparticles in ion-exchangeable polymer photonic crystals
T. Tsuruoka, S. Kumazaki, H. Nawafune, K. Akamatsu
Chem. Lett. 40 1233 - 1234 2011.8
Joint Work
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In situ generation of silver nanoparticles in ion-exchangeable polymer photonic crystals Reviewed
T.Tsuruoka, S.Kumazaki, H. Nawafune, K. Akamatsu
Chem. Lett. 40 1233 - 1234 2011.8
Joint Work
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Use of nucleic acid analogs for the study of nucleic acid interactions Invited Reviewed
S. Nakano, M. Fujii, and N. Sugimoto
Journal of Nucleic Acids 2011 ( 967098 ) 967098 2011.7
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Monolayer-Functionalized CｄTe Nanocrystals with Controlled Fluorescent Properties Based on Chemical Surface Modification
T.Tsuruoka, M.Sugiyama, R.Takahashi, H. Nawafune, K.Akamatsu
Trans.Mater.Res.Soc.Jpn 36 183 - 186 2011.6
Joint Work
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Cellular zwitterionic metabolite analogs simultaneously enhance reaction rate, thermostability, salt tolerance, and substrate specificity of alpha-glucosidase Reviewed
Eisuke Deguchi, Kazuya Koumoto
Bioorganic and Medicinal Chemistry 19 3128 - 3134 2011.4
Joint Work
Authorship：Lead author
天然の代謝産物を模倣し、化学的に合成した代謝産物アナログがα−グルコシダーゼ、β−グルコシダーゼ、アルカリフォスファターゼといった加水分解酵素の活性（反応速度、耐熱性、耐塩性、基質選択性等）を溶液に共存させるだけで向上させることを明らかとした。
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Thermodynamic stability of Hoogsteen and Watson-Crick base pairs in the presence of histone H3-mimicking peptide Reviewed
S. Pramanik, K. Nakamura, K. Usui, S. Nakano, S. Saxena, J. Matsui, D. Miyoshi and N. Sugimoto
Chem. Commun. 47 ( 10 ) 2790 - 2792 2011.2
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Thermodynamic Stability of Hoogsteen and Watson-Crick Base Pairs in the Presence of Histone H3-Mimicking Peptide Reviewed
Smritimoy Pramanik, Kaori Nakamura, Kenji Usui, Shu-ichi Nakano, Sarika Saxena, Jun Matsuia, Daisuke Miyoshi, Naoki Sugimoto
Chem. Commun. 47 2790 - 2792 2011.2
Joint Work
Authorship：Lead author
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Biodegradable Nano-aggregates of Star-Shaped 8-arm PEG-PLLA Block Co-polymers for Encapsulation of Water-Soluble Macromolecules Reviewed
K. Nagahama, T. Saito, T. Ouchi, Y. Ohya
Journal of Biomaterials Science, Polymer Edition 22 407 2011.1
Joint Work
Authorship：Lead author
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Thermodynamic Stability of Hoogsteen and Watson-Crick Base Pairs in the Presence of Histone H3-Mimicking Peptide Reviewed
Smritimoy Pramanik, Kaori Nakamura, Kenji Usui, Shu-ichi Nakano, Sarika Saxena, Jun Matsui, Daisuke Miyoshi, Naoki Sugimoto
Chemical Communications 2011
Joint Work
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Direct Detection of Thrombin Binding to 8-Bromodeoxyguanosine-Modified Aptamer: Effects of Modification on Affinity and Kinetics Reviewed
S. Goji, J. Matsui
Journal of Nucleic Acids 2011
Joint Work
Authorship：Lead author
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Monolayer-Functionalized CdTe Nanocrystals with Controlled Fluorescent Properties Based on Chemical Surface Modification
T. Tsuruoka, M. Sugiyama, R. Takahashi, H. Nawafune, K. Akamatsu
Trans. Mater. Res. Soc. Jpn 36 183 - 186 2011
Joint Work
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Synthesis of Au Nanocrystals/Porous Coordination Polymer Compoosites by Preferential Self-Assembly of Frameworks
H. Kawasaki, M. Kumano, M. Yamashita, T. Tsuruoka, H. Nawafune, K. Akamatsu
Trans. Mater. Res. Soc. Jpn. 37 181 - 184 2011
Joint Work
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In situ synthesis of comosite nanowires consisting of metal nanoparticles in ion-exchangeable polymer matrix using porous alumina tenplates
Y. Fukumoto, T. Tsuruoka, T.Yanagishita, H. Masuda, H. Nawafune, K. Akamatsu
Trans. Mater. Res. Soc. Jpn. 37 185 - 188 2011
Joint Work
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Contlloed Self-Assembly of Metal-Organic Frameworks on Metal Nanoparticles for Efficient Synthesis of Hybrid Nanostructures
T. Tsuruoka, H. Kawasaki, H. Nawafune, K. Akamatsu
ACS Appl. Mater. Interfaces 3 3788 - 3791 2011
Joint Work
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Morphology Design of Porous Coordination Polymer Crystals by Coordination Modulation
A. Umemura, S. Diring, S. Furukawa, H. Uehara, T. Tsuruoka, S. Kitagawa
J. Am.Chem.Soc 133 15506 - 15513 2011
Joint Work
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Monolayer-Functionalized CdTe Nanocrystals with Controlled Fluorescent Properties Based on Chemical Surface Modification
T. Tsuruoka, M. Sugiyama, R. Takahashi, H. Nawafune, K. Akamatsu
Trans. Mater. Res. Soc. Jpn 36 183 - 186 2011
Joint Work
-
In situ generation of silver nanoparticles in ion-exchangeable polymer photonic crystals
T. Tsuruoka, S. Kumazaki, H. Nawafune, K. Akamatsu
Chem. Lett 40 1233 - 1234 2011
Joint Work
-
Fabrication of Silver Patterns on Polyimide Films Based on Solid-Phase Electrochemical Constructive Lithography Using Ion-Exchangeable Precursor Layers
K. Akamatsu, Y. Fukumoto, T. Taniyama, T. Tsuruoka, H. Yanagimoto, H. Nawafune
Langmuir 27 11761 - 11766 2011
Joint Work
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Effect of Nickel(II) ion on Copper Electrodeposition from Ethylenediamine Complex Bath
H. Arimura, T. Shigematsu, T. Tsuruoka, K. Akamatsu, H. Nawafune
Journal of The Surface Finishing Society of Japan 62 708 - 711 2011
Joint Work
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Pyrrolidinyl peptide nucleic acid with α/β-peptide backbone - A conformationally constrained PNA with unusual hybridization properties
C. Vilaivan, C. Srisuwannaket, C. Ananthanawat, C. Suparpprom, J. Kawakami, Y. Yamaguchi, Y. Tanaka, T. Vilaivan
Artificial DNA: PNA & XNA 2 11 2011
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Thermodynamic stability of Hoogsteen and Watson-Crick base pairs in the presence of histone H3-mimicking peptide Reviewed International coauthorship
Smritimoy Pramanik, Kaori Nakamura, Kenji Usui, Shu-ichi Nakano, Sarika Saxena, Jun Matsui, Daisuke Miyoshi, Naoki Sugimoto
CHEMICAL COMMUNICATIONS 47 ( 10 ) 2790 - 2792 2011
Publisher：ROYAL SOC CHEMISTRY
We found that Hoogsteen base pairs were stabilized by molecular crowding and a histone H3-mimicking peptide, which was not observed for Watson-Crick base pairs. Our findings demonstrate that the type of DNA base pair is critical for the interaction between DNA and histones.
DOI： 10.1039/c0cc05776b
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Use of a designed peptide library to screen for binders to a particular DNA G-quadruplex sequence Reviewed
Keita Kobayashi, Noriko Matsui, Kenji Usui
Journal of Nucleic Acids 2011 572873 2011
We demonstrated a method to screen for binders to a particular G-quadruplex sequence using easily designed short peptides consisting of naturally occurring amino acids and mining of binding data using statistical methods such as hierarchical clustering analysis (HCA). Despite the small size of the library used in this study, candidates of specific binders were identified. In addition, a selected peptide stabilized the G-quadruplex structure of a DNA oligonucleotide derived from the promoter region of the protooncogene c-MYC. This study illustrates how a peptide library can be designed and presents a screening guideline for construction of G-quadruplex binders. Such G-quadruplex peptide binders could be functionally modified to enable switching, cellular penetration, and organelle-targeting for cell and tissue engineering. © 2011 Keita Kobayashi et al.
DOI： 10.4061/2011/572873
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PNA-Peptide Conjugates for Regulation of DNA and RNA G-Quadruplex Structures Depending on a Particular Protease Concentration Reviewed
Kenji Usui, Keita Kobayashi, Naoki Sugimoto
Peptides 2010 2010 608 - 609 2011
Joint Work
Authorship：Lead author
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Nanomolar aggregation of amyloid-beta peptides covalently and site-specifically modified by cholesterol oxidation products Reviewed International coauthorship
Kenji Usui, Evan T. Powers, Johan F. Paulsson, Sarah J. Siegel & Jeffery W. Kelly
Peptides 2008 2008 588 - 589 2011
Joint Work
Authorship：Lead author
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Fluorescent and Luminescent Fusion Proteins for Detection of Amyloid Beta Peptide Localization and Aggregation Reviewed
Kenji Usui, Masayasu Mie, Takashi Andou, Naoki Sugimoto, Hisakazu Mihara, Eiry Kobatake
Peptides 2010 2010 488 - 489 2011
Joint Work
Authorship：Lead author
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Designed Peptide Libraries for Protein and Cell analyses Reviewed
K. Kikuchi, K. Usui, K.-Y. Tomizaki, T. Takahashi, H. Mihara
Peptide Science 2010 2010 21 - 21 2011
Joint Work
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A Novel Nitric Oxide Sensor Using Fluorescent Peptides Attached to Iron Complexes Reviewed
Hiroshi Miyazaki, Kenji Usui, Satoshi Fujii
Peptides 2011 2011 224 - 255 2011
Joint Work
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A PNA Peptide Inducing DNAs to Form G-Quadruplex Structures Depending on a Protease Activity Reviewed
Kenji Usui, Keita Kobayashi, Naoki Sugimoto
Peptides 2011 2011 152 - 153 2011
Joint Work
Authorship：Lead author
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Nanomolar Aggregation and Neurotoxicity of Amyloid beta Peptides with Site-Specific Modification of Oxidized Cholesterol Reviewed International coauthorship
K. Usui, J. D. Hulleman, J. F. Paulsson, S. J. Siegel, E. T. Powers, J. W. Kelly
Peptide Science 2010 2010 169 - 169 2011
Joint Work
Authorship：Lead author
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Synthesis of Au Nanocrystals/Porous Coordination Polymer Compoosites by Perferential Self-Assembly of Frameworks Reviewed
H. Kawasaki, M. Kumano, M. Yamashita, T. Tsuruoka, H. Nawafune, K. Akamatsu
Trans. Mater. Res. Soc. Jpn. 37 181 - 184 2011
Joint Work
-
In situ synthesis of comosite nanowires consisting of metal nanoparticles in ion-exchangeable polymer matrix using porous alumina tenplates
Y. Fukumoto, T. Tsuruoka, T.Yanagishita, H. Masuda, H. Nawafune, K. Akamatsu
Trans. Mater. Res. Soc. Jpn. 37 185 - 188 2011
Joint Work
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Molecular Decoding using Luminescence from an Entangled Porous Framework Reviewed
Yohei Takashima, Virginia Mart?nez Mart?nez, Shuhei Furukawa, Mio Kondo, Satoru Shimomura, Hiromitsu Uehara, Masashi Nakahama, Kunihisa Sugimoto, Susumu Kitagawa
Nature Commun 2 168 - 168 2011
Joint Work
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Thermodynamically controlled coordination-engineering of novel 2D cadmium thiolate coordination polymers Reviewed
Nicolas Louvain, Yohei Takashima, Susumu Kitagawa, Shuhei Furukawa
New J. Chem. 35 1265 - 1269 2011
Joint Work
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Control of the Charge-Transfer Interaction between a Flexible Porous Coordination Host and Aromatic Guests by Framework Isomerism Reviewed
Yohei Takashima, Shuhei Furukawa, Susumu Kitagawa
CrystEngComm 13 3360 - 3363 2011
Joint Work
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Ultrasensitive and Selective Detection of a Prognostic Indicator in Early-Stage Cancer Using Graphene Oxide and Carbon Nanotubes Reviewed
Xiaohui Wang, Chunyan Wang, Konggang Qu, Yujun Song, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, and Xiaogang Qu
Advanced Functional Materials 20 3967 - 3971 2010.12
Joint Work
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Comparative thermodynamic analysis of RNA-protein interaction on surface and in solution（共著）
Y. Tanaka, K. Ishidate, K. Kishimoto, N. Sugimoto, and J. Kawakami
Proc. 37th Intl. Symp. Nucleic Acids Chemistry 276 - 277 2010.11
Joint Work
Authorship：Lead author
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A rapid and sensitive "add-mix-measure" assay for multiple proteinases based on one goldnanoparticle-peptide-fluorophore conjugate Reviewed
X.Wang, J.Geng, D.Miyoshi, J.Ren, N.Sugimoto, and X.Qu
Biosens. Bioelectron. 26 743 - 747 2010.11
Joint Work
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Stacking interaction in the middle and at the end of a DNA helix studied with non-natural nucleotides Reviewed
S. Nakano, H. Oka, Y. Uotani, K. Uenishi, M. Fujii, and N. Sugimoto
Mol. BioSyst. 6 2023 - 2029 2010.10
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Sole and stable RNA duplexes of G-rich sequences located in the 5'-untranslated region of protooncogenes Reviewed
Sarika Saxena, Daisuke Miyoshi, Naoki Sugimoto
Biochemistry 49 7190 - 7201 2010.9
Joint Work
Authorship：Lead author
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Development of new functional nanostructures consisting of both DNA duplex and quadruplex Reviewed
Kakoli Dutta, Takeshi Fujimoto, Mamiko Inoue, Daisuke Miyoshi, and Naoki Sugimoto
Chem. Commun 46 7772 - 7774 2010.9
Joint Work
Authorship：Lead author
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Controlled Multiscale Synthesis of Porous Coordination Polymer in Nano/Micro Regimes
Diring Stephane, Furukawa Shuhei, Takashima Yohei, Tsuruoka Takaaki, Kitagawa Susumu
CHEMISTRY OF MATERIALS 22 ( 16 ) 4531 - 4538 2010.8
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Anionic phthalocyanines targeting G-quadruplexes and inhibiting telomerase activity in the presence of excessive DNA duplexes Reviewed
Yaku, H., Murashima, T., Miyoshi, D., Sugimoto, N.
Chemical Communications 46 ( 31 ) 5740 - 5742 2010.8
Joint Work
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Anionic Phthalocyanines Targeting G-quadruplexes and Inhibiting Telomerase Activity in the Presence of Excessive DNA Duplexes Reviewed
Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, Naoki Sugimoto
Chem. Commun 46 5740 - 5742 2010.8
Joint Work
Authorship：Lead author
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“生きたバイオマテリアル”としての機能性細胞 Invited Reviewed
長濱宏治
バイオマテリアル 28 193 2010.8
Single Work
Authorship：Lead author
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Monomorphic RNA G-quadruplex and polymorphic DNA G-quadruplex structures responding to cellular environmental factors Reviewed
Dong-Hao Zhang, Takeshi Fujimoto, Sarika Saxena, Hai-Qing Yu, Daisuke Miyoshi, and Naoki Sugimoto
Biochemisry 49 4554 - 4563 2010.7
Joint Work
Authorship：Lead author
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Preparation of Nanoaggregates through Self-assembly of Amphiphilic Polyrotaxane Composed of PLLA–PEG–PLLA Triblock Copolymer and α-Cyclodextrins Reviewed
Koji Nagahama, Junpei Ohmura, Hiromi Sakaue, Tatsuro Ouchi, Yuichi Ohya and Nobuhiko Yui
Chemistry Letters 39 250 2010.4
Joint Work
Authorship：Lead author
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Interleukin 6 enhances glycolysis through expression of the glycolytic enzymes hexokinase 2 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3.
Ando M, Uehara I, Kogure K, Asano Y, Nakajima W, Abe Y, Kawauchi K, Tanaka N
Journal of Nippon Medical School = Nippon Ika Daigaku zasshi 77 ( 2 ) 97 - 105 2010.4
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Utilization of salmon milt DNA against UV damage Reviewed
Yoshiharu Sasaki, Daisuke Miyoshi, and Naoki Sugimoto
Applied Biochem. Biotech. 160 2458 - 2466 2010.2
Joint Work
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Upstream regulatory sequences required for specific gene expression in the ascidian neural tube. Reviewed
Shimai K, Kitaura Y, Tamari Y, Nishikata T
Zoological science 27 ( 2 ) 76 - 83 2010.2
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The molecular mechanism of glucose metabolism hampered by p53 tumor suppressor protein.
Kawauchi K
Journal of Nippon Medical School = Nippon Ika Daigaku zasshi 77 ( 1 ) 48 - 49 2010.2
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Molecular crowding of the cosolutes induces an intramolecular i-motif structure of triplet repeat DNA oligomers at neutral pH Reviewed
A. Rajendran, S. Nakano, and N. Sugimoto
Chem. Commun. 46 1299 - 1301 2010.1
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The upstream regulatory sequences required for specific gene expression in the ascidian neural tube. Reviewed
Shimai K., Kitaura Y., Tamari Y., and Nishikata T.
Zoological Science 27 76 - 83 2010
Joint Work
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Fabrication of copper damascene patterns on polyimide using direct metallization of trench templates generated by imprint lithography
T. Matsumura, Y. Enomoto, T. Tsuruoka, K. Akamatsu, H. Nawafune
Langmuir 26 12448 2010
Joint Work
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Synthesis of pH-responsive nanocomposite microgels with size-controlled gold nanoparticles from ion-doped, lightly cross-linked poly(vinylpyridine)
K. Akamatsu, M. Shimada, T. Tsuruoka, H. Nawafune, S. Fujii, Y. Nakamura
Langmuir 26 1254 2010
Joint Work
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Synthesis and characterization of polypyrrole-palladium nanocomposite-coated latex particles and their use as a catalyst for Suzuki coupling reaction in aqueous media
S. Fujii, S. Matsuzawa, Y. Nakamura, A. Ohtaka, T. Teratani, K. Akamatsu, T, Tsuruoka, H. Nawafune
Langmuir 26 6230 2010
Joint Work
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Facile one-step route to polyaniline-silver nanocomposite particles and their application as a colored particulate emulsifier
S. Fujii, Y. Nishimura, A. Aichi, S. Matsuzawa, Y. Nakamura, K. Akamatsu, H. Nawafune
Synthetic Metals 160 1322 2010
Joint Work
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Electron Magnetic Resonance in Interacting Ferromagnetic-Metal Nanoparticle Systems: Experiment and Numerical Simulation
C. Mitsumata, S. Tomita, S. Hagiwara, and K. Akamatsu
J. Phys. Cond. Mater. 22 ( 016005 ) 2010
Joint Work
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Controlled Multiscale Synthesis of Porous Coordination Polymer in Nano/Micro Regimes
S. Diring, S. Furukawa, Y. Takashima, T. Tsuruoka, S. Kitagawa
Chem. Mater 22 4531 - 4538 2010
Joint Work
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「光化学還元法によるガラス基板上への銅のダイレクトパターニング」
有村英俊、中道良太、木村明寛、鶴岡孝章、赤松謙祐、縄舟秀美
エレクトロニクス実装学会誌 13 46 - 51 2010
Single Work
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Synthesis of pH-responsive nanocomposite microgels with size-controlled gold nanoparticles from ion-doped, lightly cross-linked poly(vinylpyridine)
K. Akamatsu, M. Shimada, T. Tsuruoka, H. Nawafune, S. Fujii, Y. Nakamura
Langmuir 26 1254 - 1259 2010
Joint Work
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Synthesis and characterization of polypyrrole-palladium nanocomposite-coated latex particles and their use as a catalyst for Suzuki coupling reaction in aqueous media
S. Fujii, S. Matsuzawa, Y. Nakamura, A. Ohtaka, T. Teratani, K. Akamatsu, T, Tsuruoka, H. Nawafune
Langmuir 26 6230 - 6230 2010
Joint Work
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Facile one-step route to polyaniline-silver nanocomposite particles and their application as a colored particulate emulsifier
S. Fujii, Y. Nishimura, A. Aichi, S. Matsuzawa, Y. Nakamura, K. Akamatsu, H. Nawafune
Synthetic Metals 160 1322 - 1322 2010
Joint Work
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Fabrication of copper damascene patterns on polyimide using direct metallization of trench templates generated by imprint lithography
Y. Matsumura, Y. Enomoto, T. Tsuruoka, K. Akamatsu, H. Nawafune
Langmuir 26 12448 - 12454 2010
Joint Work
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Electron Magnetic Resonance in Interacting Ferromagnetic-Metal Nanoparticle Systems: Experiment and Numerical Simulation
C. Mitsumata, S. Tomita, S. Hagiwara, and K. Akamatsu
J. Phys. Cond. Mater 22 016005 - 016005 2010
Joint Work
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Construction of Detection System for Amyloid beta Peptide Localization and Aggregation Using Fluorescent and Luminescent Fusion Proteins Reviewed
Kenji Usui, Masayasu Mie, Takashi Andou, Naoki Sugimoto, Hisakazu Mihara, Eiry Kobatake
Peptide Science 2009 2009 111 - 112 2010
Joint Work
Authorship：Lead author
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Controlled Multiscale Synthesis of Porous Coordination Polymer in Nano/Micro Regimes Reviewed
S. Diring, S. Furukawa, Y. Takashima, T. Tsuruoka, S. Kitagawa
Chem. Mater. 22 4531 - 4538 2010
Joint Work
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光化学還元法によるガラス基板上への銅のダイレクトパターニング
有村英俊、中道良太、木村明寛、鶴岡孝章、赤松謙祐、縄舟秀美
エレクトロニクス実装学会誌 13 46 - 51 2010
Joint Work
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Synthesis of pH-Responsive Nanocomposite Microgels with Size-Controlled Gold Nanoparticles from Ion-Doped, Lightly Cross-Linked Poly(vinylpyridine) Reviewed
K. Akamatsu, M. Shimada, T. Tsuruoka, H. Nawafune, S. Fujii, Y. Nakamura
Langmuir 26 1254 - 1259 2010
Joint Work
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Synthesis and Characterization of Polypyrrole-Palladium Nanocomposite-coated Latex Particles and Their Use as a Catalyst for Suzuki Coupling Reaction in Aqueous Media Reviewed
S. Fujii, S. Matsuzawa, Y. Nakamura, A. Ohtaka, T. Teratani, K. Akamatsu, T. Tsuruoka, H. Nawafune
Langmuir 26 6230 - 6239 2010
Joint Work
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Fabrication of Copper Damascene Patterns on Polyimide Using Direct Metallization on Trench Templates Generated by Imprint Lithography Reviewed
Y. Matsumura, Y. Enomoto, T. Tsuruoka, K. Akamatsu, H. Nawafune
Langmuir 26 12448 - 12454 2010
Joint Work
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Periodic Molecular Boxes in Entangled Enantiomorphic lcy Nets Reviewed
Yohei Takashima, Charlotte Bonneau, Shuhei Furukawa, Mio Kondo, Ryotaro Matsuda, Susumu Kitagawa
Chem. Commun 46 4142 - 4144 2010
Joint Work
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Controlled Multiscale Synthesis of Porous Coordination Polymers in Nano/Micro Regimes Reviewed
St?phane Diring, Shuhei Furukawa, Yohei Takashima, Takaaki Tsuruoka, Susumu Kitagawa
Chem. Mater. 22 4531 - 4538 2010
Joint Work
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Control over the Nucleation Process determines the Framework Topology of Porous Coordination Polymers Reviewed
Mio Kondo, Yohei Takashima, Joobeom Seo, Susumu Kitagawa, Shuhei Furukawa
CrystEngComm 12 2350 - 2353 2010
Joint Work
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Stabilization of Three-Way Junctions of DNA under Molecular Crowding Conditions Reviewed
Sanjukta Muhuri, Kenta Mimura, Daisuke Miyoshi, and Naoki Sugimoto
J. Am. Chem. Soc. 131 9268 - 9280 2009.12
Joint Work
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Neuronal differentiation of neural progenitor cells by intracellular delivery of synthetic oligopeptide derived from von Hippel-Lindau protein Reviewed
H. Kanno, S. Nakano, A. Kubo, T. Mimura, N. Tajima, and N. Sugimoto
Protein & Peptide Letters 16 1291 - 1296 2009.12
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Dynamics and energetics of the base flipping conformation studied with base pair-mimic nucleosides Reviewed
S. Nakano, H. Oka, Y. Uotani, K. Uenishi, M. Fujii, and N. Sugimoto
Biochemistry 48 11304 - 11311 2009.12
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Face-to-face porphyrin moieties assembled with spacing for pyrazine recognition in molecularly imprinted polymers Reviewed
Matsui, J., Sodeyama, T., Saiki, Y., Miyazawa, T., Yamada, T., Tamaki, K., Murashima, T.
Biosensors and Bioelectronics 25 ( 3 ) 635 - 639 2009.11
Joint Work
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Hydration of Watson-Crick base pairs and dehydration of Hoogsteen base pairs inducing structural polymorphism under molecular crowding conditions
三好大輔
J. Am. Chem. Soc. ( 131 ) 3522 - 3531 2009.11
Single Work
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Site-specific modification of Alzheimer's peptides by cholesterol oxidation products enhances aggregation energetics and neurotoxicity Reviewed International coauthorship
Kenji Usui, John D. Hulleman, Johan F. Paulsson, Sarah J. Siegel, Evan T. Powers, Jeffery W. Kelly
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 106 ( 44 ) 18563 - 18568 2009.11
Publisher：NATL ACAD SCIENCES
Accumulation of amyloid beta-peptide (A beta) and tau aggregates, possibly linked to age-associated deficiencies in protein homeostasis, appear to cause Alzheimer's disease. Schiff-base formation between A beta and the aldehyde-bearing cholesterol oxidation product 3-beta-hydroxy-5-oxo-5,6-secocholestan-6-al is known to increase A beta amyloidogenicity. Here, we synthesized A beta variants site-specifically modified with the cholesterol aldehyde at Asp-1, Lys-16, or Lys-28, rather than studying mixtures. These distinct modifications have a similar effect on the thermodynamic propensity for aggregation, enabling aggregation at low concentrations. In contrast, the modification site differentially influences the aggregation kinetics; Lys-16-modified A beta formed amorphous aggregates fastest and at the lowest concentration (within 2 h at a concentration of 20 nM), followed by the Lys-28 and Asp-1 conjugates. Also, the aggregates resulting from A beta Lys-16 cholesterol aldehyde conjugation were more toxic to primary rat cortical neurons than treatment with unmodified A beta under identical conditions and at the same concentration. Our results show that A beta modification by cholesterol derivatives, especially at Lys-16, renders it kinetically and thermodynamically competent to form neurotoxic aggregates at concentrations approaching the physiologic concentration of A beta.
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Facilitation of RNA enzyme activity in the molecular crowding media of cosolutes Reviewed
S. Nakano, H. T. Karimata, Y. Kitagawa, and N. Sugimoto
J. Am. Chem. Soc. 131 16881 - 16888 2009.10
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Biodegradable poly(l-lactide)-grafted α-cyclodextrin copolymer displaying specific dye absorption by host-guest interact Reviewed
Koji Nagahama, Keiko Shimizu, Tatsuro Ouchi, Yuichi Ohya
Reactive and Functional Polymers 69 891 2009.10
Joint Work
Authorship：Lead author
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Thermo-Sensitive Sol-Gel Transition of Poly(depsipeptide-co-lactide)-g-PEG Copolymers in Aqueous Solution Reviewed
Koji Nagahama, Yuichiro Imai, Teppei Nakayama, Junpei Ohmura, Tatsuro Ouchi, Yuichi Ohya
Polymer 50 3547 2009.10
Joint Work
Authorship：Lead author
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Polyrotaxane Composed of Poly-L-Lactide and α-Cyclodextrin Exhibiting Protease-Triggered Hydrolysis Reviewed
Yuichi Ohya, Seigo Takamido, Koji Nagahama, Tatsuro Ouchi, Ryo Katoono, Nobuhiko Yui
Biomacromolecules 10 2261 2009.10
Joint Work
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Accurate curve fitting procedure for UV melting analysis of highly thermostable RNA hairpins（共著）
J. Kawakami, Y. Tanaka, and K. Kishimoto
Nucleic Acids Symp. Ser. 53 227 - 228 2009.9
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Thermal stability and hydration state of DNA G-quadruplex regulated by loop regions
T. Fujimoto, D. Miyoshi, H. Tateishi-Karimata, and N. Sugimoto
Nucleic Acids Symp Ser (Oxf) 53 237 - 238 2009.9
Joint Work
Authorship：Lead author
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DNA junction structure stabilized by molecular crowding conditions
D. Miyoshi, S. Muhuri, K. Mimura, and N. Sugimoto
Nucleic Acids Symp Ser (Oxf) 53 59 - 60 2009.9
Joint Work
Authorship：Lead author
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DNA junction structure stabilized by molecular crowding conditions
D. Miyoshi, S. Muhuri, K. Mimura, N. Sugimoto
Nucleic Acids Symp Ser (Oxf) 53 59 - 60 2009.9
Joint Work
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Investigations of the cation binding to nucleotides by monitoring the hairpin-duplex equilibrium of a self-complementary sequence
S. Nakano, H. Hirayama, and N. Sugimoto
Nucleic Acids Symp. Ser. 53 229 - 230 2009.9
Joint Work
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Porous Coordination Polymer with Pyridinium Cationic Surface, [Zn-2(tpa)(2)(Cpb)]
Higuchi Masakazu, Tanaka Daisuke, Horike Satoshi, Sakamoto Hirotoshi, Nakamura Kohei, Takashima Yohei, Hijikata Yuh, Yanai Nobuhiro, Kim Jungeun, Kato Kenichi, Kubota Yoshiki, Takata Masaki, Kitagawa Susumu
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 131 ( 30 ) 10336 - + 2009.8
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Hydrophobically Modified Biodegradable Poly(ethylene glycol) Copolymers that Form Temperature-Responsive Nanogels Reviewed
Koji Nagahama, Mihoko Hashizume, Hidetoshi Yamamoto, Tatsuro Ouchi, Yuichi Ohya
Langmuir 25 9734 2009.8
Joint Work
Authorship：Lead author
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Biodegradable Shape-Memory Polymers Exhibiting Sharp Thermal Transitions and Controlled Drug Release Reviewed
Koji Nagahama, Yuichi Ueda, Tatsuro Ouchi, Yuichi Ohya
Biomacromolecules 10 1789 2009.8
Joint Work
Authorship：Lead author
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“Geometric and electronic structure differences between the type 3 copper sites of the multicopper oxidases and hemocyanin/tyrosinase” Reviewed
Jungjoo Yoon, Satoshi Fujii, Edward I. Solomon
Proc. Natl. Acad. Sci. USA 106 ( 16 ) 6585 - 6590 2009.4
Joint Work
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Reversible stability switching of a hairpin DNA via a photo-responsive linker unit Invited Reviewed
Li Wu, Kazuya Koumoto, Naoki Sugimoto
Chemical Communications 1915 - 1917 2009.4
Joint Work
ヘアピンDNAのループ部位に導入し、光異性化によってヘアピンDNA全体の安定性を制御できる新規光応答性ユニットの開発に成功した。このユニットをヘアピン部位に導入したDNAは紫外光、可視光の照射に伴って安定性が可逆的に制御することができた。
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Nuclease Resistant Methylphosphonate-DNA/LNA Chimeric Oligonucleotides Reviewed
Koji Nagahama, Rakesh Veedu, Jesper Wengel
Bioorganic and Medicinal Chemistry Letters 19 2707 2009.3
Joint Work
Authorship：Lead author
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Loss of p53 enhances catalytic activity of IKKbeta through O-linked beta-N-acetyl glucosamine modification.
Kawauchi K, Araki K, Tobiume K, Tanaka N
Proceedings of the National Academy of Sciences of the United States of America 106 ( 9 ) 3431 - 3436 2009.3
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Effects of Polydepsipeptide Side-Chain Groups on the Temperature Sensitivity of Triblock Copolymers Composed of Polydepsipeptides and Poly(ethylene glycol) Reviewed
Yuichi Ohya, Hidetoshi Yamamoto, Koji Nagahama, Tatsuro Ouchi
Journal of Polymer Science, Part A: Polymer Chemistry 47 3892 2009.2
Joint Work
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DNAは細胞内で三重らせん・四重らせん構造を形成しているのか？ Invited
三好大輔、杉本直己
化学と生物 7 449 - 451 2009.1
Joint Work
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Cu(II) ion inhibits early stages of amyloid β fibril formation in vitro
T. Fujimoto, D. Miyoshi, H. Tateishi-Karimata, and N. Sugimoto
J. Biol. Inorg. Chem., Suppl. 1 S118 2009.1
Joint Work
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An Approach to Peptide-based ATP Receptors by a Combination of Random Selection, Rational Design, and Molecular Imprinting Reviewed
J. Matsui, J. Nagano, D. Miyoshi, K. Tamaki, N. Sugimoto
Biosens. Bioelectron. 25 563 - 567 2009.1
Joint Work
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Cu(II) ion inhibits early stages of amyloid β fibril formation in vitro
T. Fujimoto, D. Miyoshi, H. Tateishi-Karimata, N. Sugimoto
J. Biol. Inorg. Chem., Suppl. 1 S118 2009.1
Joint Work
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DNAは細胞内で三重らせん・四重らせん構造を形成しているのか？
三好大輔, 杉本直己
化学と生物 7 449 - 451 2009.1
Joint Work
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Molecularly Imprinted Nanocomposites for Highly Sensitive SPR Detection of a Non-aqueous Atrazine Sample Reviewed
J. Matsui, M. Takayose, K. Akamatsu, H. Nawafune, K. Tamaki, N. Sugimoto
Analyst 2009
Joint Work
Authorship：Lead author
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Face-to-face Porphyrin Moieties Assembled with Spacing for Pyrazine Recognition in Molecularly Imprinted Polymers Reviewed
J. Matsui, T. Sodeyama, Y. Saiki, T. Miyazawa, T. Yamada, K. Tamaki, T. Murashima
Biosensors and Bioelectronics 2009
Joint Work
Authorship：Lead author
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An Approach to Peptide-based ATP Receptors by a Combination of Random Selection, Rational Design, and Molecular Imprinting Reviewed
J. Matsui, J. Nagano, D. Miyoshi, K. Tamaki, N. Sugimoto
Biosensors and Bioelectronics 2009
Joint Work
Authorship：Lead author
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Interaction between Oocyte and Accessory Cells during Ascidian Oogenesis.
Shimai K., Ishii H., and Nishikata T.
Mem. Konan Univ., Sci. Eng. Ser. 56 31 - 41 2009
Joint Work
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ベンゾトリアゾール誘導体のマイクロコンタクトプリンティングを利用するULSI銅微細配線の形成
有村英俊、尾山祐斗、鶴岡孝章、赤松謙祐、縄舟秀美
表面技術 60 598 - 602 2009
Joint Work
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Enhancement of photoluminescence from silicon nanocrystals by metal nanostructures made by nanosphere lithography
Y. Mochizuki, M. Fujii, S. Hayashi, T. Tsuruoka, K. Akamatsu
J. Appl. Phys. 106 013517 2009
Joint Work
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Site-selective direct nickel metallization on precursor polyimide using microcontact printing
Y. Matsumura, Y. Enomoto, K. Akamatsu, H. Nawafune
J. Surf, Finish, Soc. Jpn. 60 61 - 62 2009
Joint Work
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Molecularly imprinted nanocomposites for highly sensitive SPR detection of an non-aqueous atrazine sample
J. Matui, M. Takayose, K. Akamatsu, H. Nawafune, K. Tamaki, K. Sugimoto
Analyst 134 80 - 86 2009
Joint Work
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A designed peptide chip: Protein fingerprinting technology with a dry peptide array and statistical data mining Reviewed
Kenji Usui, Kin-Ya Tomizaki, Hisakazu Mihara
Methods in Molecular Biology 570 273 - 284 2009
There has recently been increased interest in the potential for microarray technologies to study protein networks in a whole cell system within a single experiment. Protein-detecting microarrays are composed of numerous agents immobilized within a tiny area on solid surfaces to capture targeted proteins and to detect interactions in a high-throughput fashion. In this chapter, in order to extend the usability of peptide microarrays, we describe a novel dry peptide microarray format to obtain protein fingerprint (PFP) data sets and a statistical PFP data manipulation technique to quantitatively analyze targeted proteins. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.
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Nanoporous Nanorods Fabricated by Coordination Modulation and Oriented Attachment Growth Reviewed
T. Tsuruoka, S. Furukawa, Y. Takashima, K. Yoshida, S. Isoda, S. Kitagawa
Angew. Chem. Int. Ed. 48 4739 - 4743 2009
Joint Work
印刷中
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Heterogeneously Hybridized Porous Coordination Polymer Crystals: Fabrication of Heterometallic Core–Shell Single Crystals with an In-Plane Rotational Epitaxial Relationship Reviewed
S. Furukawa, K. Hirai, K. Nakagawa, Y. Takashima, R. Matsuda, T. Tsuruoka, M. Kondo, R. Haruki, D. Tanaka, H. Sakamoto, S. Shimomura, O. Sakata, S. Kitagawa
Angew. Chem. Int. Ed. 48 1766 - 1770 2009
Joint Work
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A Block PCP Crystal: Anisotropic Hybridization of Porous Coordination Polymers by Face-Selective Epitaxial Growth Reviewed
S. Furukawa, K. Hirai, Y. Takashima, K. Nakagawa, M. Kondo, T. Tsuruoka, O. Sakata, S. Kitagawa
Chem. Commun. 5097 - 5099 2009
Joint Work
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ベンゾトリアゾール誘導体のマイクロコンタクトプリンティングを利用するULSI銅微細配線の形成 Reviewed
有村英俊、尾山祐斗、鶴岡孝章、赤松謙祐、縄舟秀美
表面技術 60 598 - 602 2009
Joint Work
-
Enhancement of Photoluminescence from silicon nanocrystals by metal nanostructures made by nanosphere lithography Reviewed
Y. Mochizuki, M. Fujii, S. Hayashi, T. Tsuruoka, K. Akamatsu
J. Appl. Phys. 106 013517 2009
Joint Work
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Heterogeneously Hybridized Porous Coordination Polymer Crystals: Fabrication of Heterometallic Core-Shell Single Crystals with an In-Plane Rotational Epitaxial Relationship Reviewed
Shuhei Furukawa, Kenji Hirai, Keiji Nakagawa, Yohei Takashima, Ryotaro Matsuda, Takaaki Tsuruoka, Mio Kondo, Rie Haruki, Daisuke Tanaka, Hirotoshi Sakamoto, Satoru Shimomura, Osami Sakata and Susumu Kitagawa
Angew. Chem. Int. Ed 48 1766 - 1770 2009
Joint Work
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A Block PCP Crystal: Anisotropic Hybridization of Porous Coordination Polymers by Face-Selective Epitaxial Growth Reviewed
Shuhei Furukawa, Kenji Hirai, Yohei Takashima, Keiji Nakagawa, Mio Kondo, Takaaki Tsuruoka, Osami Sakata, Susumu Kitagawa
Chem. Commun 5097 - 5099 2009
Joint Work
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Porous Coordination Polymer with Pyridinium Cationic Surface, [Zn2(tpa)2(cpb)] Reviewed
Masakazu Higuchi, Daisuke Tanaka, Satoshi Horike, Hirotoshi Sakamoto, Kohei Nakamura, Yohei Takashima, Yuh Hijikata, Nobuhiro Yanai, Jungeun Kim, Kenichi Kato, Yoshiki Kubota, Masaki Takata, Susumu Kitagawa
J. Am. Chem. Soc 131 10336 - 10337 2009
Joint Work
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Nanoporous Nanorods Fabricated by Coordination Modulation and Oriented Attachment Growth Reviewed
Takaaki Tsuruoka, Shuhei Furukawa, Yohei Takashima, Kaname Yoshida, Seiji Isoda and Susumu Kitagawa
Angew. Chem. Int. Ed 48 4739 - 4743 2009
Joint Work
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Design of allele-specific primers and detection of the human ABO genotyping to avoid the pseudopositive problem Reviewed
H. Yaku, T. Yukimasa, S. Nakano, N. Sugimoto, and H. Oka
Electrophoresis, 29 4130 - 4140 2008.12
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Structural effect of synthetic zwitterionic cosolutes on the stability of DNA duplexes Reviewed
甲元一也
Tetrahedron ( 64 ) 168 - 174 2008.11
Joint Work
Authorship：Lead author
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Small-molecule-directed assembly of DNA: a gold-nanoparticle-based strategy for screening of a DNA duplex binders
三好大輔
Adv. Mat ( 20 ) 706 - 710 2008.11
Single Work
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Telomerase activity regulated by thermodynamic stability of DNA・RNA hybrid
三好大輔
Angew. Chem. Int. Ed., ( 47 ) 9034 - 9038 2008.11
Single Work
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Temperature-Induced Hydrogel through Self-Assembly of Cholesterol-substituted Star PEG-b-PLLA Copolymers as an Injectable Scaffold for Tissue Engineering Reviewed
K. Nagahama, T. Ouchi, Y. Ohya,
Adv. Funct. Mater. 18 ( 8 ) 1220 - 1231 2008.11
Joint Work
Authorship：Lead author
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Biodegradable Nanogels Prepared by Self-Assembly of Poly(L-Lactide)-Grafted Dextran: Entrapment and Release of Protein Reviewed
K. Nagahama, T. Ouchi, Y. Ohya,
Macromol. Biosci. 8 ( 11 ) 1044 - 1952 2008.11
Joint Work
Authorship：Lead author
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Irreversible Temperature-Responsive Formation of High-strength Hydrogel from an Enantiomeric Mixture of Starburst Triblock Copolymers Consisting of 8-arm PEG and PLLA or PDLA Reviewed
K. Nagahama, K. Fujiura, S. Enami, T. Ouchi, Y. Ohya,
J. Polym. Sci. Part A Polym. Chem., 46 ( 18 ) 6317 - 6332 2008.11
Joint Work
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Resolution of secondary alcohols via Carica papaya lipase-catalyzed enantioselective acylation Reviewed
Miyazawa, T., Houhashi, M., Inoue, Y., Murashima, T., Yamada, T.
Biotechnology Letters 30 ( 10 ) 1783 - 1787 2008.10
Joint Work
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Peptide synthesis using carbamoylmethyl esters as acyl donors mediated by Bacillus amyloliquefaciens protease Reviewed
Miyazawa, T., Shindo, T., Murashima, T., Yamada, T.
Protein and Peptide Letters 15 ( 10 ) 1050 - 1054 2008.10
Joint Work
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Molecular crowding effect on metal ion binding properties of the hammerhead ribozyme
S. Nakano, Y. Kitagawa, H. T. Karimata, and N. Sugimoto
Nucleic Acids Symp. Ser. 52 519 - 520 2008.9
Joint Work
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IKK/NF-kappaB signaling pathway inhibits cell-cycle progression by a novel Rb-independent suppression system for E2F transcription factors. Reviewed
Araki K, Kawauchi K, Tanaka N
Oncogene 27 ( 43 ) 5696 - 5705 2008.9
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Activated p53 induces NF-kappaB DNA binding but suppresses its transcriptional activation.
Kawauchi K, Araki K, Tobiume K, Tanaka N
Biochemical and biophysical research communications 372 ( 1 ) 137 - 141 2008.7
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Conformation and the sodium ion condensation on DNA and RNA structures in the presence of a neutral cosloute as a mimic of the intracellular media Reviewed
Shuichi Nakano, Lei Wu, Hirohito Oka, Hisae Tateishi-Karimata, Toshimasa Kirihata, Yuichi Sato, Satoshi Fujii, Hiroshi Sakai, Masayuki Kuwahara, Hiroaki Sawai, and Naoki Sugimoto
Mol. BioSys. 4 579 - 588 2008.6
Joint Work
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Cycloprodigiosin hydrochloride activates the Ras-PI3K-Akt pathway and suppresses protein synthesis inhibition-induced apoptosis in PC12 cells.
Kawauchi K, Tobiume K, Iwashita K, Inagaki H, Morikawa T, Shibukawa Y, Moriyama Y, Hirata H, Kamata H
Bioscience, biotechnology, and biochemistry 72 ( 6 ) 1564 - 1570 2008.6
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Cationic Porphyrin Induced a Telomeric DNA to G-Quadruplex Form in Water. Reviewed
Murashima, T., Sakiyama, D., Miyoshi, D., Kuriyama, M., Yamada, T., Miyazawa, T., Sugimoto, N.
Bioinorganic Chemistry and Applications 2008.5
Joint Work
Authorship：Lead author
Bioinorg. Chem. Appl. （学術雑誌、2008） 2008, Article ID 294756,
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p53 regulates glucose metabolism through an IKK-NF-kappaB pathway and inhibits cell transformation.
Kawauchi K, Araki K, Tobiume K, Tanaka N
Nature cell biology 10 ( 5 ) 611 - 618 2008.5
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“Reaction of iron(III)-polyaminocarboxylate complexes with hydrogen peroxide: Correlation between ligand structure and reactivity.” Reviewed
Satoshi Fujii, Chiyo Tsueda, Kunio Yamabe, Kohsuke Nakajima, and Hiroshi Sakai
Inorg. Chim. Acta 361 1207 - 1211 2008.3
Joint Work
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Design of Polylactide-Based Hydrogels as Biodegradable Soft-Biomaterials
Koji NAGAHAMA
2008.3
Single Work
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Hedgehog signaling overrides p53-mediated tumor suppression by activating Mdm2.
Abe Y, Oda-Sato E, Tobiume K, Kawauchi K, Taya Y, Okamoto K, Oren M, Tanaka N
Proceedings of the National Academy of Sciences of the United States of America 105 ( 12 ) 4838 - 4843 2008.3
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Capture of cationic ligands bound diffusely to base pairs during the DNA refolding
S. Nakano, T. Kirihata, and N. Sugimoto
Chem. Commun. ( 6 ) 700 - 702 2008.2
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Conformation and the sodium ion condensation on DNA and RNA structures in the presence of a neutral cosolute as a mimic of the intracellular media Invited Reviewed
S. Nakano, L. Wu, H. Oka, H. T. Karimata, T. Kirihata, Y. Sato, S. Fujii, H. Sakai, M. Kuwahara, H. Sawai, and N. Sugimoto
Mol. BioSyst. 4 579 - 588 2008.2
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Recognition of a flipped base in a hairpinloop DNA by a small peptide （共著） Reviewed
J. Kawakami, S. Okabe, Y. Tanabe, and N. Sugimoto
Nucleoside, Nucleotides and Nucleic Acids 27 292 - 308 2008.2
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Riboswitches for enhancing target gene expression in eukaryotes Reviewed
T. Yamauchi, D. Miyoshi, T. Kubodera, M. Ban, A. Nishimura, and N. Sugimoto
ChemBioChem 9 1040 - 1043 2008.1
Joint Work
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外部刺激に応答する機能性核酸 －核酸ならではの機能を活用したナノバイオマテリアルの設計－ Invited
三好大輔、杉本直己
化学工業 （化学工業社） 59 ( 11 ) 21 - 27 2008.1
Joint Work
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Thermodynamics of DNA structures under molecular crowding conditions with neutral and positive charged cosolutes
D. Miyoshi, K. Nakamura, S. Muhuri, H. T. Karimata, N. Sugimoto
Nucleic Acids Symp. Ser. 52 413 - 414 2008.1
Joint Work
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Cationic porphyrin induced a telomeric DNA to g-quadruplex form in water Reviewed
T. Murashima, D. Sakiyama, D. Miyoshi, M. Kuriyama, T. Yamada, T. Miyazawa, N. Sugimoto
Bioinorg Chem Appl. 294756 2008.1
Joint Work
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Molecular crowding effects on structure and stability of DNA Invited Reviewed
D. Miyoshi and N. Sugimoto
Biochimie 90 1040 - 1051 2008.1
Joint Work
-
外部刺激に応答する機能性核酸 －核酸ならではの機能を活用したナノバイオマテリアルの設計－
三好大輔, 杉本直己
化学工業 （化学工業社） 59 ( 11 ) 21 - 27 2008.1
Joint Work
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Highly regioselective propanoylation of dihydroxybenzenes mediated by Candida antarctica lipase B in organic solvents Reviewed
Miyazawa, T., Hamada, M., Morimoto, R., Murashima, T., Yamada, T.
Tetrahedron Letters 49 ( 1 ) 175 - 178 2008.1
Joint Work
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“Spectroscopic Studies of Perturbed T1 Cu Sites in the Multicopper Oxidases Saccharomyces cerevisiae Fet3p and Rhus vernicifera Laccase: Allosteric Coupling between the T1 and Trinuclear Cu Sites” Reviewed
Anthony J. Augustine, Mads Emil Kragh, Ritimukta Sarangi, Satoshi Fujii, Barry D. Liboiron, Christopher S. Stoj, Daniel J. Kosman, Keith O. Hodgson, Britt Hedman, and Edward I. Solomon
Biochemistry 47 ( 7 ) 2036 - 2045 2008.1
Joint Work
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Preparation and Characterization of Au Nanoparticle/Polymer composite Photonic Crystals
T. Tsuruoka, S. Samitsu, T. Aokata, J. Matsui, T. Murashima, K. Akamatsu, H. Nawafune
Trans.MRS-Japan 717 - 720 2008
Joint Work
-
Novel ubiquitous promoters and expression-vector optimization in ascidian embryos.
西方敬人
Invertebrate Reproduction & Development ( 51 ) 103 - 110 2008
Single Work
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Multimodal pathways during UVB-induced apoptosis in HL-60.
西方敬人
Proceedings of the 14th Biennial Congress of the International Society for Free Radical Research, Beijing, China 2008
Single Work
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Nanocomposite polymeric microspheres containing Ni nanoparticles with controlled microstructures
K. Akamatsu, S. Adachi, T. Tsuruoka, S. Ikeda, S. Tomita, H. Nawafune
Chem. Mater. 20 3042 - 3047 2008
Joint Work
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Immobilization and Assembly of Mixed Monolayer-Protected Au Nanoparticles on Glass substrate via Covalent Bond Interconnection
T. Tsuruoka, A. Fujimoto, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn. 33 157 - 160 2008
Joint Work
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Highly luminescent mono- and multilayers of immobilized CdTe nanocrystals: controlling optical properties through post chemical surface modification
T. Tsuruoka, R. Takahashi, T. Nakamura, M. Fujii, K. Akamatsu, and H. Nawafune
Chem. Commun. 1641 - 1643 2008
Joint Work
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Control of the optical properties of CdTe nanocrystals by selective exchange of Te with thiolate: effect of organic ligands on the formation of core-shell structures
T. Tsuruoka, R. Takahashi, K. Akamatsu, H. Nawafune
Phys. Chem. Chem. Phys. 10 2221 - 2226 2008
Joint Work
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Direct metallization of nickel on polymeric template patterns for fabrication of copper circuits on glass substrates
Y. Matsumura, Y. Enomoto, M. Sugiyama, K. Akamatsu, H. Nawafune
J. Mater. Chem. 18 5058 - 5082 2008
Joint Work
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Fabrication of conductive thin films on precursor polyimide via chemical reduction of nickel (II) complex
Y. Matsumura, Y. Enomoto, T. Jyo, K. Akamatsu, H. Nawafune
J. Surf, Finish, Soc. Jpn. 59 562 - 564 2008
Joint Work
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Formation of metal thin film nanocomposite layer from precursor polyimide containing metal complex
M. Matsumura, Y. Enomoto, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn. 33 1333 - 1336 2008
Joint Work
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Molecular crowding effect on metal ion binding properties of the hammerhead ribozyme
S. Nakano, Y. Kitagawa, H. T. Karimata, and N. Sugimoto
Nucleic Acids Symp. Ser. 52 519 - 520 2008
Joint Work
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Nanocomposite Polymeric Microspheres Containing Ni Nanoparticles with Controlled Microstructures Reviewed
K. Akamatsu, S. Adachi, T. Tsuruoka, S. Ikeda, S. Tomita, H. Nawafune
Chem. Mater. 20 3042 - 3047 2008
Joint Work
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Highly Luminescent Mono- and Multilayers of Immobilized CdTe Nanocrystals: Controlling Optical Properties Through Post Chemical Surface Modification Reviewed
T. Tsuruoka, R. Takahashi, T. Nakamura, M. Fujii, K. Akamatsu, H. Nawafune
Chem. Commun. 1641 - 1643 2008
Joint Work
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Control of the Optical Properties of CdTe Nanocrystals by Selective Exchange of Te with Thiolate: Effect of Organic Ligands on the Formation of Core-Shell Structures Reviewed
T. Tsuruoka, R. Takahashi, K. Akamatsu, H. Nawafune
Phys. Chem. Chem. Phys. 10 2221 - 2226 2008
Joint Work
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Immobilization and Assembly of Mixed Monolayer-Protected Au Nanoparticles on Glass Substrate via Covalent Bond Interconnection Reviewed
T. Tsuruoka, A. Fujimoto, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn. 33 157 - 160 2008
Joint Work
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Preparation and Characterization of Au Nanoparticles/Polymer Composite Photonic Crystals Reviewed
T. Tsuruoka, S. Samitsu, T. Aokata, J. Matui, T. Murashima, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn. 33 717 - 720 2008
Joint Work
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Influence of cationic molecules on the hairpin to duplex equilibria of self-complementary DNA and RNA oligonucleotides Reviewed
S. Nakano, T. Kirihata, S. Fujii, H. Sakai, M. Kuwahara, H. Sawai, and N. Sugimoto
Nucleic Acids Research 35 486 - 494 2007.12
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Synthesis, structure and thermal stability of fully hydrophobic porphyrin-DNA conjugates.
Murashima, T., Hayata, K., Saiki, Y., Matsui, J., Miyoshi, D., Yamada, T., Miyazawa, T., Sugimoto, N.
Tetrahedron Lett., 48 ( 48 ) 8514 - 8517 2007.11
Joint Work
Authorship：Lead author
Tetrahedron Lett., （学術雑誌、2007）48, 8514-8517.
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Secondary alcohols act as better nucleophiles than primary alcohols in the lipase-catalyzed regioselective deacylation of dihydroxybenzenes acylated at both phenolic hydroxyls.
Miyazawa, T., Hamada, M., Morimoto, R., Murashima, T., Yamada, T.
Tetrahedron Lett., 48 ( 47 ) 8334 - 8337 2007.11
Joint Work
Tetrahedron Lett., （学術雑誌、2007）48, 8334-8337.
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Artificial G-wire Switch with 2,2’-Bipyridine Units Responsive to Divalent Metal Ions
甲元一也
J. Am. Chem. Soc ( 129 ) 5919 - 5925 2007.11
Single Work
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５）天然高分子 核酸 エネルギーデータベースに基づく核酸医薬の設計
甲元一也
絵で見てわかるナノDDS 遺伝子医学MOOK別冊（メディカルドゥ） 63 - 69 2007.11
Single Work
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Conformational switch of a functional nanowire based on the DNA G-quadruplex
甲元一也
Nucleic Acids Symp. Ser. ( 51 ) 251 - 252 2007.11
Single Work
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Delivery of antisense oligonucleotides to nuclear telomere RNA by use of a complex between polysaccharide and polynucleotide
甲元一也
Bull. Chem. Soc. Jpn ( 80 ) 1091 - 1098 2007.11
Single Work
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Molecular Imprinting Under Molecular Crowding Conditions: an Aid to the synthesis of a High-capacity Polymeric Sorbent for Triazine Herbicides
三好大輔
Anal. Chem ( 79 ) 1749 - 1757 2007.11
Single Work
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DNAを用いたバイオ計測 －センシング核酸の開発と新展開－
三好大輔
未来材料, 7 ( 6 ) 10 - 18 2007.11
Single Work
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Regulation of DNA nucleases by molecular crowding
三好大輔
Nucleic Acids Res ( 35 ) 4086 - 4093 2007.11
Single Work
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Artificial G-wire Switch with 2,2’-Bipyridine Units Responsive to Divalent Metal Ions
三好大輔
J. Am. Chem. Soc ( 129 ) 5919 - 5925 2007.11
Single Work
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DNAデザイン －機能性分子開発への道しるべ－
三好大輔
化学 62 ( 9 ) 28 - 32 2007.11
Single Work
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DNA四重らせん構造を細胞内環境因子で制御する
三好大輔
生体機能関連化学部会 News Letter, 22 ( 3 ) 14 - 17 2007.11
Single Work
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Synthesis, structure and thermal stability of fully hydrophobic porphyrin-DNA conjugates
三好大輔
Tetrahedron Lett ( 48 ) 8514 - 8517 2007.11
Single Work
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Effects of cosolutes on the thermodynamic stability of parallel DNA duplex and triplex
三好大輔
Nucleic Acids Symp. Ser. (Oxf), ( 51 ) 167 - 168 2007.11
Single Work
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Conformational switch of a functional nanowire based on the DNA G-quadruplex
三好大輔
Nucleic Acids Symp. Ser. (Oxf), ( 51 ) 167 - 168 2007.11
Single Work
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DNA四重らせん構造を細胞内環境因子で制御する
三好大輔
生体機能関連化学部会 News Letter, 22 ( 3 ) 14 - 17 2007.11
Single Work
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Effects of polyethylene glycol on a DNA duplex stability differed by the NaCl concentration Reviewed
H. Karimata, S. Nakano, and N. Sugimoto
Bull. Chem. Soc. Jpn. 80 1987 - 1994 2007.11
Joint Work
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The base-pairing ability of the base pair-mimic nucleosides
S. Nakano, K. Uenishi, M. Fujii, and N. Sugimoto
Nucleic Acids Symp. Ser. 51 71 - 72 2007.11
Single Work
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Impacts of Stereoregularity and Stereocomplex Formation on Physicochemical, Protein Adsorption and Cell Adhesion Behaviors of Star-Shaped 8-Arms Poly(ethylene glycol)-Poly(lactide) Block Copolymer Films Reviewed
K. Nagahama, Y. Nishimura, Y. Ohya,
Polymer, 48 ( 9 ) 2649 - 2658 2007.11
Joint Work
Authorship：Lead author
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Exhibition of Soft and Tenacious Characters Based on Liquid Crystal Formation by Introduction of Cholesterol Groups on Biodegradable Lactide Copolymer Reviewed
K. Nagahama, Y. Ueda, T. Ouchi, Y. Ohya
Biomacromolecules, 8 ( 12 ) 3938 - 3943 2007.11
Joint Work
Authorship：Lead author
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Molecular “Screw and Nut”: α-Cyclodextrin Recognizes Polylactide Chirality Reviewed
Y. Ohya, S. Takamido, K. Nagahama, T. Ouchi, T. Ooya, R. Katoono, N. Yui
Macromolecules, 40 ( 18 ) 6441 - 6444 2007.11
Joint Work
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Biodegradable Nanogel Formation of Polylactide-Grafted Dextran Copolymer in Dilute Aqueous Solution and Enhancement of Its Stability by Stereocomplexation, Reviewed
K. Nagahama, Y. Mori Y. Ohya, T. Ouchi
Biomacromolecules 8 ( 7 ) 2135 - 2141 2007.11
Joint Work
Authorship：Lead author
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Functionalization of DNA by the base pair-mimic nucleosides
H. Oka, S. Nakano, Y. Uotani, K. Uenishi, M. Fujii, and N. Sugimoto
Nucleic Acids Symp. Ser. 51 151 - 152 2007.11
Joint Work
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Suppression of AP-1 activity by cycloprodigiosin hydrochloride.
Kawauchi K, Tobiume K, Kaneko S, Kaneshiro K, Okamoto S, Ueda E, Kamata H, Moriyama Y, Hirata H
Biological & pharmaceutical bulletin 30 ( 9 ) 1792 - 1795 2007.9
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Schizophyllan, beta-1,3-glucan,-oligoamine conjugates as non-viral vector Reviewed
Takeshi Nagasaki, Atsushi Uno, Kazuya Koumoto, Kazuo Sakurai, Seiji Shinkai
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 234 2007.8
Publisher：AMER CHEMICAL SOC
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Consecutive GC base pairs determine the energy barrier of DNA duplex formation under molecularly crowded conditions Reviewed
X. Gu, S. Nakano, and N. Sugimoto
Chem. Commun. 2750 - 2752 2007.4
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Mechanistic characterization of the HDV genomic ribozyme: A mutant of the C41 motif provides insight into positioning and thermodynamic linkage of metal ions and protons Reviewed
S. Nakano and P. C. Bevilacqua
Biochemistry 46 3001 - 3012 2007.2
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Molecular Imprinting Under Molecular Crowding Conditions: an Aid to the synthesis of a High-capacity Polymeric Sorbent for Triazine Herbicides.
Matsui, J., Goji, S., Murashima, T., Miyoshi, D., Komai, S., Shigeyasu, A., Kushida, T
Anal. Chem., 79 ( 4 ) 1749 - 1757 2007.2
Joint Work
Anal. Chem., （学術雑誌、2007）79, 1749-1757.
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Hydration regulates thermodynamic stability of DNA structures under molecular crowding conditions Reviewed
D. Miyoshi, H. Karimata, and N. Sugimoto
Nucleosides, Nucleotides and Nucleic Acids 26 589 - 595 2007.1
Joint Work
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“Modulation of the DNA and RNA Secondary Structure by Cationic Molecule” Reviewed
Shu-ichi Nakano, Toshimasa Kirihata, Satoshi Fujii, Hiroshi Sakai, Masayuki Kuwahara, Hiroaki Sawai, and Naoki Sugimoto
Nucleic Acids Res 35 ( 2 ) 486 - 494 2007.1
Joint Work
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Screening of alpha-helical peptide ligands controlling a calcineurin-phosphatase activity Reviewed
Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 17 ( 1 ) 167 - 171 2007.1
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
In this paper, we describe an application of 202-membered fluorescently labeled peptide library designed to take an alpha-helix secondary structure. As a proof-of-concept experiment, a calmodulin (CaM)/calcineurin (Cn) pair was chosen to screen alpha-helical peptide ligands that tightly bind to CaM and also control enzymatic functions of Cn. Three peptides were successfully selected from the library by assaying Cn-phosphatase activities and peptide-CaM interactions (dual check process). The strategy using a designed peptide. library shows real promise as a peptide-based high-throughput screening system. (c) 2006 Elsevier Ltd. All rights reserved.
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Molecular Imprinting Under Molecular Crowding Conditions: an Aid to the synthesis of a High-capacity Polymeric Sorbent for Triazine Herbicides
J. Matsui, S. Goji, T. Murashima, D. Miyoshi, S. Komai, A. Shigeyasu, T. Kushida, T. Miyazawa, T. Yamada, K. Tamaki, N. Sugimoto
Anal. Chem. 2007
Joint Work
Authorship：Lead author
-
Synthesis, structure and thermal stability of fully hydrophobic porphyrin-DNA conjugates Reviewed
T. Murashima, K. Hayata, Y. Saiki, J. Matsui, D. Miyoshi, T., Yamada, T. Miyazawa, and N. Sugimoto
Tetrahedron Letters 2007
Joint Work
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Neural-tube specific paralogous genes and their upstream regulatory sequences
西方敬人
Mem. Konan Univ., Sci. Eng. Ser ( 54 ) 75 - 88 2007
Single Work
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Controlled magnetic properties of Ni nanoparticles embedded in polyimide films
S. Tomita, P. E. Jonsson, K. Akamatsu, H. Nawafune, H. Takayama
Phys. Rev. B ( 174432 ) 1 - 6 2007
Joint Work
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ポリエチレングリコール誘導体の予備吸着を利用する銅電析プロセスによるULSI微細配線の形成
尾山祐斗、有村英俊、赤松謙祐、縄舟秀美
表面技術 58 425 - 429 2007
Joint Work
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Copper/polyimide heterojunction: controlling interfacial structures through an additive-based, all-wet chemical process using ion-doped precursors
S. Ikeda, H. Yanagimoto, K. Akamatsu, H. Nawafune
Adv. Funct. Mater. 17 889 - 897 2007
Joint Work
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One-step synthesis of polypyrrole-coated silver nanocomposite particles and their application as a coloured particulate emulsifier
S. Fujii, A. Aichi, K. Akamatsu, H. Nawafune and Y. Nakamura
J. Mater. Chem. 17 3777 - 3779 2007
Joint Work
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Atomic Beam-Induced Fluorination of Polyimide and Its Application to Site-Selective Cu Metallization
M. Tagawa, K. Maeda, T. Kajita, K. Yokota, K. Akamatsu, H. Nawafune
Langmuir 23 11351 - 11354 2007
Joint Work
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Photosensitization of oxygen molecules by surface-modified hydrophilic porus Si
M. Fujii, N. Nishimura, H. Fumon, S. Hayashi, K. Akamatsu, T. Tsuruoka, M. Shimada, H. Katayama, D. Kovalev, B. Goller
Euro. Phys. J. D 43 193 - 196 2007
Joint Work
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Interactions between peptides containing nucleobase amino acids and T7 phages displaying S-cerevisiae proteins Reviewed
Sinya Watanabe, Kin-ya Tomizaki, Tsuyoshi Takahashi, Kenji Usui, Kotaro Kajikawa, Hisakazu Mihara
BIOPOLYMERS 88 ( 2 ) 131 - 140 2007
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Photosensitization of Oxygen Molecules by Surface-Modified Hydrophilic Porus Si Reviewed
M. Fujii, N. Nishimura, H. Fumon, S. Hayashi, K. Akamatsu, T. Tsuruoka, M. Shimada, H. Katayama, D. Kovalev, B. Goller
Euro. Phys. J. D 43 193 - 196 2007
Joint Work
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Functional role of the cofactor on activation process of L-histidine dependent ribozyme（共著）
J. Kawakami, C. Maiya, and N. Sugimoto
Nucleic Acids Symp. Ser. 50 241 - 242 2006.11
Joint Work
Authorship：Lead author
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Galactose-PEG Dual Conjugation of b-(1→3)-D-glucan schizophyllan for antisense oligonucleotide delivery to enhance cellular uptake
甲元一也
Biomaterials, ( 27 ) 1626 - 1635 2006.11
Single Work
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CpG DNA/zymosan complex to enhance cytokine secretion owing to the cocktail effect
甲元一也
Bioorg. Med. Chem. Lett ( 16 ) 1301 - 1304 2006.11
Single Work
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A DNA duplex with Extremely Enhanced Thermal Stability Based on Controlled Immobilization on Gold Nanoparticles.
三好大輔
Nano Lett., ( 6 ) 491 - 495 2006.11
Single Work
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Development of molecular logic gates using the structural switch of telomere DNAs
三好大輔
Nucleic Acids Symp. Ser. ( 50 ) 315 - 316 2006.11
Single Work
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Molecularly Imprinting within a Single Peptide Molecule for Synthesizing a Water-soluble ATP Receptor
三好大輔
Proceedings of the International Conference of 43rd Japanese Peptide Symposium and 4th Peptide Engineering Meeting 396 - 397 2006.11
Single Work
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Properties of long telomeric DNAs under cell-mimicking conditions
三好大輔
Nucleic Acids Symp. Ser. ( 50 ) 207 - 208 2006.11
Single Work
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Factors regulating thermodynamic stability of DNA structures under molecular crowding conditions
三好大輔
Nucleic Acids Symp. Ser ( 50 ) 203 - 204 2006.11
Single Work
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Characterization of Structure and Stability of Long Telomeric DNA G-Quadruplexes
三好大輔
J. Am. Chem. Soc., ( 128 ) 15461 - 15468 2006.11
Single Work
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DNA Logic Gates Based on Structural Polymorphism of Telomere DNA Molecules Responding to Chemical Input Signals
三好大輔
Angew. Chem. Int. Ed., ( 45 ) 7716 - 7719 2006.11
Single Work
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Hydration regulates thermodynamics of G-quadruplex formation under molecular crowding conditions
三好大輔
J. Am. Chem. Soc ( 128 ) 7957 - 7963 2006.11
Single Work
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生命を制御するのは遺伝子か環境か－細胞内環境に左右される遺伝子の構造と機能－
三好大輔
高分子 55 ( 5 ) 322 - 325 2006.11
Single Work
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Effect of molecular crowding on DNA polymerase activity
三好大輔
Biotech. J., ( 1 ) 440 - 446 2006.11
Single Work
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Properties of long telomeric DNAs under cell-mimicking conditions
三好大輔
Nucleic Acids Symp. Ser. ( 50 ) 207 - 208 2006.11
Single Work
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The Suppression of Cell and Platelet Adhesion to Star-Shaped Poly(ethylene glycol)-Poly(L-lacitde) Block Copolymer Film, Reviewed
K. Nagahama, Y. Ohya, T. Ouchi,
Macromol. Biosci. 6 ( 6 ) 412 - 419 2006.11
Joint Work
Authorship：Lead author
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Synthesis of Star-Shaped 8arms Poly(ethylene glycol)-Poly(L-lacitde) Block Copolymer and Physical Properties of Its Solution Cast Film as Soft Biomaterial Reviewed
K. Nagahama, Y. Ohya, T. Ouchi,
Polym. J., 38 ( 8 ) 852 - 860 2006.11
Joint Work
Authorship：Lead author
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The roles of cosolutes on the hammerhead ribozyme activity
中野 修一
Nucleic Acids Symp. Ser. 50 81 - 82 2006.11
Joint Work
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The effect of the structure of cosolutes on the DNA duplex formation
中野 修一
Nucleic Acids Symp. Ser. 50 205 - 206 2006.11
Joint Work
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Recognition of the base pair-mimic nucleosides by DNA polymerases
中野 修一
Nucleic Acids Symp. Ser. 50 201 - 202 2006.11
Joint Work
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Protein-fingerprint data mining of a designed alpha-helical peptide array Reviewed
Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara
MOLECULAR BIOSYSTEMS 2 ( 9 ) 417 - 420 2006.9
Publisher：ROYAL SOC CHEMISTRY
The data generated from protein fingerprints with an a-helical peptide array were analyzed using several statistical methods such as hierarchical clustering analysis and principal component analysis to discriminate target proteins. The data generated from protein fingerprints with an a-helical peptide array were analyzed using several statistical methods such as hierarchical clustering analysis and principal component analysis to discriminate target proteins.
DOI： 10.1039/b608875a
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A novel stable RNA pentaloop that interacts specifically with a motif peptide of lambda-N protein（共著） Reviewed
J. Kawakami, H. Tokitoh, Y. Tanabe, and N. Sugimoto
Nucleoside, Nucleotides and Nucleic Acids 25 397 - 416 2006.4
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The negative regulation of p53 by hedgehog signaling Reviewed
Abe Yoshinori, Oda-Sato Eri, Tobiume Kei, Kawauchi Keiko, Okamoto Koji, Taya Yoichi, Tanaka Nobuyuki
CANCER RESEARCH 66 ( 8 ) 2006.4
Joint Work
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A novel peptide microarray for protein detection and analysis utilizing a dry peptide array system Reviewed
K Usui, K Tomizaki, T Ohyama, K Nokihara, H Mihara
MOLECULAR BIOSYSTEMS 2 ( 2 ) 113 - 121 2006.2
Publisher：ROYAL SOC CHEMISTRY
A novel dry peptide microarray system has been constructed that affords a practical solution for protein detection and analysis. This system is an array preparation and assay procedure tinder dry conditions that uses designed peptides as non-immobilized capture agents for the detection of proteins. The system has several advantages that include its portability and ease-Of-Use, as well as the fact that vaporization of sample solutions need not be considered. In this study, various proteins have been characterized with an a-helical peptide mini-library. When proteins were added to the peptide library array, the fluorescent peptides showed different fluorescent intensities depending on their sequences. The patterns of these responses could be regarded as protein fingerprints' (PFPs), which are sufficient to establish the identities of the target proteins. Furthermore, statistical analysis of the resulting PFPs was performed using cluster analysis. The PFPs of the proteins were clustered successfully depending on their families and binding properties. Additionally, the target protein was characterized using a nanolitre system and could be detected down to 1.2 fmol. These Studies imply that the dry peptide array system is a promising too] for detecting and analyzing target proteins. The dry peptide array will play a role in development of high-throughput protein-detecting nano/micro arrays for proteomics and ligand screening studies.
DOI： 10.1039/b514263r
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A PNA-DNA hybridization chip approach for the detection of beta-secretase activity Reviewed
S Sano, KY Tomizaki, K Usui, H Mihara
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 16 ( 3 ) 503 - 506 2006.2
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
Developed was the addressable chip technology based on the PNA-DNA complementary hybridization equipped with short seven-mer PNA-encoded peptides that can be a versatile scaffold to monitor on-chip immunoassays. We also developed and validated a methodology to perform beta-secretase enzyme assay with a highly sensitive fashion, resulting that a peptide substrate tethering dual fluorescent probes allowed us to detect beta-secretase activity 10 times more sensitively than assays in solution. (c) 2005 Elsevier Ltd. All rights reserved.
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A DNA duplex with extremely enhanced thermal stability based on controlled immobilization on gold nanoparticles Reviewed
K. Akamatsu, M. Kimura, Y. Shibata, S. Nakano, D. Miyoshi, H. Nawafune, and N. Sugimoto
Nano Lett. 3 491 - 495 2006.1
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Use of the polymorphic nature of high-order DNA structures for the design of dynamic and functional nanodevices Invited Reviewed
D. Miyoshi, N. Sugimoto
Recent Devel. Nucleic Acids Res. 2 49 - 70 2006.1
Joint Work
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Use of the polymorphic nature of high-order DNA structures for the design of dynamic and functional nanodevices
D. Miyoshi, N. Sugimoto
Recent Devel. Nucleic Acids Res. 2 49 - 70 2006.1
Joint Work
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Photodimerization of Anthryl Moieties in a Poly(methacrylic acid) Derivative as Reversible Cross-linking Step in Molecular Imprinting
J. Matsui, Y. Ochi, K. Tamaki
Chem. Lett. 80 - 81 2006
Joint Work
Authorship：Lead author
-
Molecularly-Imprinted Polymeric Logic Gates Selective for Predetermined Chemical Input Species
松井淳
Chem. Comm. 3217 - 3219 2006
Single Work
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Dynamic redistribution of vasa homolog and exclusion of somatic cell determinants during germ cell specification in Ciona intestinalis.
西方敬人
Development ( 133 ) 2683 - 2693 2006
Single Work
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UV-irradiated cells as a screening system for the antioxidants.
西方敬人
Proceedings of the 13th Biennial Congress of the International Society for Free Radical Research -SFRR 253 - 256 2006
Single Work
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A DNA duplex with Extremely Enhanced Thermal Stability Based on Controlled Immobilization on Gold Nanoparticles
K. Akamatsu, M. Kimura, Y. Shibata, S. Nakano, D. Miyoshi, H. Nawafune, N. Sugimoto
Nano Lett. 6 491 - 495 2006
Joint Work
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金属イオンレドックス系無電解めっき浴の安定性
赤松謙祐、増田昭仁、尾山祐斗、縄舟秀美
表面技術 57 799 - 801 2006
Joint Work
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Fabrication of fluorescent multilayers consisting of CdTe nanocrystals
T. Tsuruoka, R. Takahashi, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn. 31 425 - 428 2006
Joint Work
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Designed peptide microarrays for protein detection and characterization Reviewed
Kenji Usui, Kin-ya Tomizaki, Kiyoshi Nokihara, Hisakazu Mihara
UNDERSTANDING BIOLOGY USING PEPTIDES 731 - + 2006
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High throughput preparation of peptide arrays containing two fluorescent byes focusing on practical protein detection systems Reviewed
Kiyoshi Nokihara, Takafumi Ohyama, Koichi Yonemura, Yasuo Oka, Kenji Usui, Hisakazu Mihara
UNDERSTANDING BIOLOGY USING PEPTIDES 738 - + 2006
Joint Work
Publisher：SPRINGER
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Site-Selective Integration of Monolayer-Protected Inorganic Nanoparticles onto Surface Monolayer Templates by a Solvent-Induced Lift-Off Process Reviewed
K. Akamatsu, S. Samitsu, T. Tsuruoka, J. Hasegawa, H. Nawafune
Small 2 1130 - 1133 2006
Joint Work
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Fabrication of Fluorescent Multilayers Consisting of CdTe Nanocrystals Reviewed
T. Tsuruoka, R. Takahashi, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn. 31 425 - 428 2006
Joint Work
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DNA base flipping by a base pair-mimic nucleoside Reviewed
S. Nakano, Y. Uotani, K. Uenishi, M. Fujii, and N. Sugimoto
Nucleic Acids Research, 33 7111 - 7119 2005.12
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Anomalous reflection of gold applicable for a practical protein-detecting chip platform Reviewed
S Watanabe, K Usui, K Tomizaki, K Kajikawa, H Mihara
MOLECULAR BIOSYSTEMS 1 ( 5-6 ) 363 - 365 2005.12
Publisher：ROYAL SOC CHEMISTRY
A simple, convenient and label-free fiber optic detection system based on the characteristic property, 'anomalous reflection (AR)' of gold was developed and preliminary experiments showed that the AR signals were sensitive enough to monitor protein-peptide interactions on solid surfaces.
DOI： 10.1039/b513075c
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Amino Acid Ester Salt Recognition by Ferrocene-based Ditopic Receptor Bearing Oligoethylene Glycol with Pendant Bipy Subunits: CV, UV-vis and ESR Studies. Reviewed
Murashima, T., Tsukiyama, S., Fujii, S., Hayata, K., Sakai, H., Miyazawa, T., Yamada, T.
Org. Biomol. Chem., 3 ( 22 ) 4060 - 4064 2005.11
Joint Work
Authorship：Lead author
Org. Biomol. Chem., （学術雑誌、2005）3, 4060-4064.
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“Amino acid ester salt recognition by ferrocene-based ditopic receptor bearing oligoethylene glycol with pendant bipy subunits: CV, UV-vis and ESR Studies” Reviewed
Takashi Murashima, Sayo Tsukiyama, Satoshi Fujii, Kazuyuki Hayata, Hiroshi Sakai, Toshifumi Miyazawa, and Takashi Yamada
Org. Biomol. Chem 3 4060 - 4064 2005.11
Joint Work
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In situ monitoring of polysaccharide-polynucleotide interaction using a schizophyllan-immobilized QCM device
甲元一也
Sensors & Actuators B: Chemicals ( 105 ) 490 - 494 2005.11
Single Work
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Transferrin-appended b-(1→3)-D-glucan schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake
甲元一也
J. Contr. Rel. ( 108 ) 529 - 539 2005.11
Single Work
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Transferrin-appended b-(1→3)-D-glucan schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake
甲元一也
e-J. Surface Sci. Nanotech ( 3 ) 195 - 202 2005.11
Single Work
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Cholesterol-appended b-(1,3)-D-glucan schizophyllan for antisense oligonucleotide delivery to enhance cellular uptake
甲元一也
Bull. Chem. Soc. Jpn. ( 78 ) 1821 - 1830 2005.11
Single Work
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Parallel vs. anti-parallel orientation in a curdlan/oligo(dA) complex as estimated by a FRET technique
甲元一也
Org. Biomol. Chem., ( 3 ) 2255 - 2261 2005.11
Single Work
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Competition between Polysaccharide/Polynucleotide Complexation vs. Polynucleotide Hybridization; Salt Concentration Dependence of the Reaction Direction
甲元一也
e-J. Surface Sci. Nanotech. ( 3 ) 30 - 37 2005.11
Single Work
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PEG-appended b-(1→3)-D-glucan schizophyllan to deliver antisense-oligonucleotides with avoiding lysosomal degradational of New Modification Technique for Linear Double-Stranded DNAs Using the Polysaccharide Schizophyllan
甲元一也
Biomaterials ( 26 ) 4866 - 4873 2005.11
Single Work
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Schizophyllan-Poly(N-isopropylacrylamide) Conjugate for Selective Recovery of Polynucleotides by Precipitation
甲元一也
Polym. J., ( 37 ) 177 - 185 2005.11
Single Work
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Thermodynamic and Kinetic Analysis of Nucleic Acid Structures toward Pharmacogenomics.
三好大輔
Current Pharmacogenomics, ( 3 ) 217 - 236 2005.11
Single Work
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Structural switch of telomere DNA by pH and monovalent cation.
三好大輔
Nucleic Acids Symp. Ser ( 49 ) 243 - 244 2005.11
Single Work
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Structure and stability of DNA quadruplexes under molecular crowding conditions.
三好大輔
Nucleic Acids Symp. Ser ( 49 ) 239 - 240 2005.11
Single Work
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DNA nanowire sensitive to the surrounding condition.
三好大輔
Nucleic Acids Symp. Ser. ( 49 ) 43 - 44 2005.11
Single Work
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DNA-Based Biosensor for Monitoring pH in Vitro and in Living Cells.
三好大輔
Biochemistry ( 44 ) 7125 - 7130 2005.11
Single Work
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Roles of Mg2+ in TPP-dependent riboswitch.
三好大輔
FEBS Lett. ( 579 ) 2583 - 2588 2005.11
Single Work
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Drastic Effect of a Single Base Difference between Human and Tetrahymena Telomere Sequences on Their Structures under Molecular Crowding Conditions.
三好大輔
Angew. Chem. Int. Ed. Engl ( 44 ) 3740 - 3744 2005.11
Single Work
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SPR sensor chip for detection of small molecules using molecularly imprinted polymer with embedded gold nanoparticles.
三好大輔
Anal Chem ( 77 ) 4282 - 4285 2005.11
Single Work
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Roles of Mg2+ in TPP-dependent riboswitch.
三好大輔
FEBS Lett ( 579 ) 2583 - 2588 2005.11
Single Work
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Thermodynamic and Kinetic Analysis of Nucleic Acid Structures toward Pharmacogenomics
三好大輔
Current Pharmacogenomics ( 3 ) 217 - 236 2005.11
Single Work
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Drastic effect of a single base difference between human and tetrahymena telomere sequences on their structures under molecular crowding conditions.
三好大輔
Angew Chem Int Ed Engl. ( 44 ) 3740 - 3744 2005.11
Single Work
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SPR Sensor Chip for Detection of Small Molecules Using Molecularly Imprinted Polymer with Embedded Gold Nanoparticles Anal. Chem.
松井淳
（学術雑誌、 2005 ）77, 4282-4285 4282 - 4285 2005.11
Single Work
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Synthesis of Water-soluble Porphyrin and the Corresponding Highly Planar Benzoporphyrin without meso-Substituents.
村嶋 貴之
Tetrahedron Lett., 2005.11
Single Work
Tetrahedron Lett., （学術雑誌、2005）46, 113-116.
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Resolution of Non-protein Amino Acids via Carica papaya Lipase-catalyzed Enantioselective Transesterification.
村嶋 貴之
Tetrahedron: Asymmetry, 2005.11
Single Work
Tetrahedron: Asymmetry, （学術雑誌、2005）16, 2569-2573.
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Enzymatic Resolution of 2-Aryloxy-1-propanols via Lipase-catalyzed Enantioselective Acylation Using Acid Anhydrides as Acyl Donors.
村嶋 貴之
J. Mol. Cat., B, 2005.11
Single Work
J. Mol. Cat., B, （学術雑誌、2005）37, 63-67.
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Studies on intramolecular hydrogen bonding between the pyridine nitrogen and the amide hydrogen of the peptide: synthesis and conformational analysis of tripeptides containing novel amino acids with a pyridine ring.
村嶋 貴之
J. Peptide Sci., 2005.11
Single Work
J. Peptide Sci., （学術雑誌、2005）11, 491-498.
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Conformational switch of oligonucleotides induced by spermine
中野 修一
Nucleic Acids Symp. Ser. 49 241 - 242 2005.11
Single Work
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Linkage between proton binding and folding in RNA: implications for RNA catalysis
中野 修一
Biochem. Soc. Trans. 33 466 - 470 2005.11
Single Work
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Kenji Usui, Tetsunori Ojima, Kin-Ya Tomizaki, Hisakazu Mihara
Nanobiotechnology 1 ( 2 ) 191 - 199 2005.11
For the realization of a practical high-throughput protein detection and analysis system, a novel peptide array has been constructed using a designed glycopeptide model library with an α-helical secondary structure. This study will contribute the increment of the diversity of such an array system and the application to focused proteomics and ligand screening by effective detection of sugar-binding proteins. Fluorescent glycopeptides with an α-helix, a β-strand, or a loop structure were designed initially to select a suitable scaffold for the detection of a model protein. After selection of the α-helical structure as the best scaffold, a small model library with various saccharides was constructed to have charge and hydrophobicity variations in the peptide sequences. When various sugar-binding proteins were added to the peptide library array, the fluorescent peptides showed different responses in fluorescence intensities depending on their sequences as well as saccharides. The patterns of these responses could be regarded as "protein fingerprints" (PFPs), which are able to establish the identities of the target proteins. The resulting PFPs reflected the recognition properties of the proteins. Furthermore, statistical data analysis from obtained PFPs was performed using a cluster analysis. The PFPs of sugar-binding proteins were clustered successfully depending on their families and binding properties. These studies demonstrate that arrays with glycopeptide libraries based on designed structures can be promising tools to detect and analyze the target proteins. Designed peptides with functional groups such as sugars will play roles as the capturing agents of high-throughput protein nano/micro arrays for focused proteomics and ligand screening studies. Copyright © 2005 Humana Press Inc. All rights of any nature whatsoever are reserved.
DOI： 10.1385/NBT:1:2:191
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Difference in self-assembling morphology of peptide nanorings Reviewed
H Okamoto, T Yamada, H Miyazaki, T Nakanishi, K Takeda, K Usui, Obataya, I, H Mihara, H Azehara, W Mizutani, K Hashimoto, H Yamaguchi, Y Hirayama
JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS 44 ( 11 ) 8240 - 8248 2005.11
Publisher：JAPAN SOC APPLIED PHYSICS
We synthesized the peptide nanorings of cyclo[-(D-Ala-L-Gln)(3)], cyclo[-(D-CyS-L-Gln)(3)], CyC10[-D-Cys-L-HiS-D-Ala-L-Asn-Gly-L-Gln-1 and Cyc1o[-(L-Gln)(5)], and studied the way in which the difference in the type and/or number of component amino acid residues changes the self-assembling morphology of the nanorings on gold substrates by atomic force microscopy. The study revealed that CyClo[-(D-Ala-L-Gln)(3)] formed nanotube bundles through inter-ring hydrogen bonds, while the nanorings of CyC10[-(D-CyS-L-Gln)3] adhered to the gold surface directly due to the high affinity of thiol to gold. In contrast, a random amino acid sequence of cyclo[-D-CyS-L-HiS-D-Ala-L-Asn-GlY-L-Gln-] resulted in many isolated nanotubes, which were first observed in the present study. While the D,L-peptide nanotubes have very straight forms, the homo-L-peptide of cyclo[-(L-Gln)(5)] formed interesting randomly branching nanotubes that were entwined and grew on the substrate. Scanning tunneling microscopy was also performed and high-resolution images of both the peptide nanotubes and the nanotube bundles were obtained.
DOI： 10.1143/JJAP.44.8240
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IR study on stacking manner of peptide nanorings in peptide nanotubes Reviewed
Y Nagai, T Nakanishi, H Okamoto, K Takeda, Y Furukawa, K Usui, H Mihara
JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS 44 ( 10 ) 7654 - 7661 2005.10
Publisher：JAPAN SOC APPLIED PHYSICS
We here report our theoretical as well as experimental studies on the stacking manner of peptide nanorings (PNRs) in peptide nanotubes (PNTs). We focus on the molecular vibrations of N-H and C=O stretching modes and discuss this subject via their infrared (IR) spectroscopy, because PNTs are formed by the inter-ring H bonds between the adjacent PNRs via -N-H(...)O=C-. Symmetry analysis based on group theory reveals that parallel stacking causes two IR active modes in these molecular vibrations while three modes are active in the antiparallel stacking. This difference in the number of IR active modes is determined only by the stacking manner and not by the number of amino acid residues composing the PNRs. By using two typical PNRs Of cyclo[-((L)-Gln-D-Ala)(3)] and cyclo[-((L)-Gln-(D)-Ala)(4)], we further studied the favorable stacking manners of PNRs via IR observation. Our IR experiments as well as the ab initio energetics show that the former PNRs create a PNT by stacking themselves in parallel while the latter PNRs do so by stacking themselves in an antiparallel manner.
DOI： 10.1143/JJAP.44.7654
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Specific recognition of the basic N peptide by an RNA（共著）
J. Kawakami, H. Tokitoh, Y. Tanabe, and N. Sugimoto
Nucleic Acids Symposium Series 49 353 - 354 2005.9
Joint Work
Authorship：Lead author
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Central dogma for a molecular design based on DNA: DNB (databasing/designable nanobio) -> ENB (engineering nanobio) -> FNB (functional nanobio) Reviewed
S. Nakano and N. Sugimoto
Chemistry Letters 34 1206 - 1211 2005.7
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Protein-detecting microarrays: Current accomplishments and requirements Reviewed
Kin-Ya Tomizaki, Kenji Usui, Hisakazu Mihara
ChemBioChem 6 ( 6 ) 782 - 799 2005.5
Joint Work
The sequencing of the human genome has been successfully completed and offers the chance of obtaining a large amount of valuable information for understanding complex cellular events simply and rapidly in a single experiment. Interestingly, in addressing these proteomic studies, the importance of protein-detecting microarray technology is increasing. In the coming few years, microarray technology will become a significantly promising and indispensable research/diagnostic tool from just a speculative technology. It is clear that the protein-detecting microarray is supported by three independent but strongly related technologies (surface chemistry, detection methods, and capture agents). Firstly, a variety of surface-modification methodologies are now widely available and offer site-specific immobilization of capture agents onto surfaces in such a way as to keep the native conformation and activity. Secondly, sensitive and parallel detection apparatuses are being developed to provide highly engineered microarray platforms for simultaneous data acquisition. Lastly, in the development of capture agents, antibodies are now probably the most prominent capture agents for analyzing protein abundances. Alternative scaffolds, such as phage-displayed antibody and protein fragments, which provide the advantage of increasing diversity of proteinic capture agents, however, are under development. An approach involving recombinant proteins fused with affinity tag(s) and coupled with a highly engineered surface chemistry wilt provide simple production protocols and specific orientations of capture agents on the -microarray formats. Peptides and other small molecules can be employed in screening highly potent ligands as well as in measuring enzymatic activities. Protein-detecting microarrays supported by the three key technologies should contribute in accelerating diagnostic/biological research and drug discovery. © 2005 Wiley-VCH Verlag GmbH &
Co. KGaA. -
Studies on Fluorescent Dyes and Surface Chemistry focusing on Practical Peptide Array Preparation Reviewed
NOKIHARA Kiyoshi, USUI Kenji, YONEMURA Koichi, OHYAMA Takafumi, OKA Yasuo, TOMIZAKI Kin‐ya, MIHARA Hisakazu
Pept Sci 2004 145 - 148 2005.3
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Tyrosine phosphorylation of adaptor protein 3BP2 induces T cell receptor-mediated activation of transcription factor.
Qu X, Kawauchi-Kamata K, Miah SM, Hatani T, Yamamura H, Sada K
Biochemistry 44 ( 10 ) 3891 - 3898 2005.3
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Thermodynamic analysis of duplex formation of the heterochiral DNA with L-deoxyadenosine（共著） Reviewed
J. Kawakami, K. Tsujita, and N. Sugimoto
Analytical Sciences 21 ( 2 ) 77 - 82 2005.2
Joint Work
Authorship：Lead author
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Site-selective RNA cleavage by DNA bearing a base pair-mimic nucleoside Reviewed
S. Nakano, Y. Uotani, K. Uenishi, M. Fujii, and N. Sugimoto
J. Am. Chem. Soc. 127 518 - 519 2005.1
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Nucleic acid with guanidinium modification exhibits efficient cellular uptake.
西方敬人
Angew. Chem. Int. Ed. Engl ( 44 ) 6682 - 6685 2005
Single Work
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Site-Selective Deposition of Gold on Glass Substrates Using Monolayer-Protected Gold Nanoparticles
K. Akamatsu, H. Terahata, J. Hasegawa, H. Nawafune
J. Surf, Finish, Soc. Jpn 56 427 - 428 2005
Joint Work
（学術雑誌、2005）56, 427-428
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Fabrication and Structural Control of Fe/Ti Oxide Thin Films with Graded Compositional Profiles by Liquid Phase Deposition
S. Deki, S. Iizuka, K. Akamatsu, M. Mizuhata and A. Kajinami
J. Ame. Ceram. Soc. 88 731 - 736 2005
Joint Work
（学術雑誌、2005) 88, 731-736
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Selective Electroless Copper Deposition on Aluminum Nitride Substrate with Patterned Copper Seed Layer
H. Yanagimoto, S. Deki, K. Akamatsu, and K. Gotoh
Thin Solid Films 491 18 - 22 2005
Joint Work
（学術雑誌、2005) 491, 18-22
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A novel fabrication technique for interacting ferromagnetic-metal nanoparticle systems:fine-tuning of particle dimeter and interparticle spacing
S. Tomita, K. Akamatsu, H. Shinkai, S. Ikeda, H. Nawafune, C. Mitsumata, T. Kashiwagi, M. Hagiwara
Mater. Res. Soc. Proceedings 853E I5.10.1 - I5.10.6 2005
Joint Work
（学術雑誌、2005） 853E. I5.10.1-I5.10.6
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Site-Selective Deposition of Gold on Glass Substrates Using Monolayer-Protected Gold Nanoparticles
K. Akamatsu, H. Terahata, J. Hasegawa, H. Nawafune
J. Surf. Finish. Soc. Jpn 56 427 - 428 2005
Joint Work
（学術雑誌、2005）56. 427-428
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Band Gap Engineering of CdTe nanocrystals Through Chemical Surface Modification
K. Akamatsu, T. Tsuruoka, H. Nawafune
J. Am. Chem. Soc. 127 1634 - 1635 2005
Joint Work
（学術雑誌、2005） 127. 1634-1635
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Stereocontrolled Synthesis and Optical Properties of All-cis Poly(phenylene vinylene)s (PPVs): A Method for Direct Patterning of PPVs
H. Katayama, M. Nagao, T. Nishimura, Y. Matsui, K. Umeda, K. Akamatsu, T. Tsuruoka, H. Nawafune, F. Ozawa
J. Am. Chem. Soc. 127 4350 - 4353 2005
Joint Work
（学術雑誌、2005） 127. 4350-4353
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An SPR Sensor Chip for Sensitive Detection of Small Molecules Using Molecularly Imprinted Polymer with Embedded Gold Nanoparticle
J. Matsui, K. Akamatsu, N. Hara, D. Miyoshi, H. Nawafune, K. Tamaki, N. Sugimoto
Anal. Chem. 77 4282 - 4285 2005
Joint Work
（学術雑誌、2005） 77. 4282-4285
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Controlling Interparticle Spacing Among Metal Nanoparticles Through Metal-Catalyzed Decomposition of Surrounding Polymer Matrix
K. Akamatsu, H. Shinkai, S. Ikeda, S. Adachi, H. Nawafune, S. Tomita
J. Am. Chem. Soc. 127 7980 - 7981 2005
Joint Work
（学術雑誌、2005） 127. 7980-7981
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Tuning magnetic interactions in ferromagnetic-metal nanoparticle systems
S. Tomita, K. Akamatsu, H. Shinkai, S. Ikeda, H. Nawafune, C. Mitsumata, T. Kashiwagi, and M, Hagiwara
Phys. Rev. B 71 180414(R)1 - 4 2005
Joint Work
（学術雑誌、2005） 71. 180414(R)1-4
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Surface Functionalization of Mixed Monolayer-Protected Semiconductor Nanocrystals
T. Tsuruoka, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc 30 1227 - 1230 2005
Joint Work
（学術雑誌、2005） 30, 1227-1230
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Synthesis and Structural Control of Metal-Containing Polyimide Nanocomposite
H. Shinkai, S. Ikeda, K. Akamatsu, H. Nawafune, S. Tomita
Trans. Mater. Res. Soc. Jpn. 30 1223 - 1226 2005
Joint Work
（学術雑誌、2005）30, pp. 1223-1226
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Site-Selective Direct Photochemical Deposition of Copper on Glass Substrates Using TiO2 Nanocrystals
K. Akamatsu, A. Kimura, H. Matsubara, S. Ikeda, H. Nawafune
Langmuir 21 8099 - 8102 2005
Joint Work
（学術雑誌、2005）21, 8099-8102
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Protein-detection microarrays using structure-based designed peptide libraries Reviewed
Kenji Usui, Tetsunori Ojima, Masato Suzuki, Sin-ya Watanabe, Kin-ya Tomizaki, Kiyoshi Nokihara, Hisakazu Mihara
Peptides 2004, Proceedings 401 - 402 2005
Joint Work
Publisher：KENES INT
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Optimization of peptide arrays on chip as novel practical protein characterization system Reviewed
Kiyoshi Nokihara, Takafumi Ohyama, Koichi Yonemura, Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara
Peptides 2004, Proceedings 176 - 177 2005
Joint Work
Publisher：KENES INT
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Band Gap Engineering of CdTe Nanocrystals Through Chemical Surface Modification Reviewed
K. Akamatsu, T. Tsuruoka, H. Nawafune
J. Am. Chem. Soc. 127 1634 - 1635 2005
Joint Work
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Stereocontrolled Synthesis and Optical Properties of All-cis Poly(phenylene vinylene)s (PPVs): A Method for Direct Patterning of PPVs Reviewed
H. Katayama, M. Nagao, T. Nishimura, Y. Matsui, K. Umeda, K. Akamatsu, T. Tsuruoka, H. Nawafune, F. Ozawa
J. Am. Chem. Soc. 127 4350 - 4353 2005
Joint Work
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Surface Functionalization of Mixed Monolayer-Protected Semiconductor Nanocrystals Reviewed
T. Tsuruoka, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn. 30 1227 - 1230 2005
Joint Work
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Nickel- and Palladium-Catalyzed Cross-Coupling Reaction of Polyfluorinated Arenes and Alkenes with Grignard Reagents Reviewed
Tomoyuki Saeki, Yohei Takashima, Kohei Tamao
Synlett 11 1771 - 1774 2005
Joint Work
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Duplex dissociation of telomere DNAs induced by molecular crowding. Reviewed
D. Miyoshi, S. Matsumura, S. Nakano, and N. Sugimoto
J. Am. Chem. Soc. 128 165 - 169 2004.12
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Introduction of peptide function into DNA by Nucleic Acid Peptides, NAPs（共著）
J. Kawakami, Z.-M. Wang, H. Fujiki, S. Izumi, and N. Sugimoto
Chemistry Letters 33 ( 12 ) 1554 - 1555 2004.12
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多糖・核酸複合体を用いた機能性高分子コンジュゲート −核酸医薬デリバリーへの挑戦−
甲元一也
高分子加工 ( 53 ) 561 - 568 2004.11
Single Work
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A Polysaccharide Carrier for Immunostimulatory CpG DNAs to Enhance Cytokine Secretion
甲元一也
J. Am. Chem. Soc. ( 126 ) 8372 - 8373 2004.11
Single Work
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Removal of the side-chain glucose groups from schizophyllan improves the thermal stability of the polycytidylic acid complex under the physiological conditions
甲元一也
Biopolymers ( 75 ) 403 - 411 2004.11
Single Work
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Schizophyllans carrying oligosaccharide appendages as potential candidates for cell-targeted antisense carrier
甲元一也
Org. Biomol. Chem ( 2 ) 3091 - 3098 2004.11
Single Work
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Proposal of New Modification Technique for Linear Double-Stranded DNAs Using the Polysaccharide Schizophyllan
甲元一也
Bioorg. Med. Chem. Lett ( 14 ) 5655 - 5659 2004.11
Single Work
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Molecular weight control of curdlan (b-1,3-glucan polysaccharide) provides unique polynucleotide binding properties
甲元一也
Polym. J. ( 36 ) 380 - 385 2004.11
Single Work
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Low Mw sulfated curdlan with improved water-solubility forms macromolecular complexes with polycytidylic acid
甲元一也
Carbohydr. Res ( 339 ) 161 - 167 2004.11
Single Work
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Chemically modified polysaccharide schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake efficiency
甲元一也
Biochim. Biophys. Acta ( 1670 ) 91 - 104 2004.11
Single Work
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Lactose-appended schizophyllan as a potential candidate for hepatocyte-targetted antisense carrier
甲元一也
Chem. Commun 382 - 383 2004.11
Single Work
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Protection of polynucleotides against nuclease-mediated hydrolysis by complexation with schizophyllan
甲元一也
Biomaterials ( 25 ) 3109 - 3116 2004.11
Single Work
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Antisense oligonucleotides bound in the polysaccharide complex and the enhanced antisense effect due to the low hydrolysis
甲元一也
Biomaterials ( 25 ) 3117 - 3123 2004.11
Single Work
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Long-Term Expression with Cationic Polymer Derived from a Natural Polysaccharide: Schizophyllan
甲元一也
Bioconjugate Chem ( 15 ) 249 - 259 2004.11
Single Work
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Polysaccharide/Polynucleotide Complexes Part 6: Complementary-Strand-Induced Release of Single-stranded DNA Bound in the Schizophyllan Complex
甲元一也
Chemistry & Biodiversity ( 1 ) 520 - 529 2004.11
Single Work
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Enhancement of antisense effect by reducing interactions between proteins and ODNs
甲元一也
Bull. Chem. Soc. Jpn. ( 77 ) 1101 - 1110 2004.11
Single Work
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First observation by Fluorescence Polarization of Complexation between mRNA and the Natural Polysaccharide Schizophyllan
甲元一也
Chemistry & Biodiversity ( 1 ) 634 - 639 2004.11
Single Work
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Duplex Dissociation of Telomere DNA Induced by Molecular Crowding.
三好大輔
J. Am. Chem. Soc. ( 126 ) 165 - 169 2004.11
Single Work
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生命分子を用いたサブナノデバイスの創製
三好大輔
未来材料(NTS) ( 4 ) 26 - 33 2004.11
Single Work
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Composite of Au Nanoparticles and molecularly imprinted polymer as a sensing material
三好大輔
Anal. Chem ( 76 ) 1310 - 1315 2004.11
Single Work
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Synthetic Cinchonidine Receptors Obtained by Cross-linking Linear Poly(Methacrylic Acid) Derivatives as an Alternative Molecular Imprinting Technique J. Chromatogr., B
松井淳
（学術雑誌、 2004 ）804, 223-229 223 - 229 2004.11
Single Work
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Atrazine Tranformation Using Synthetic Enzymes Prepared by Molecular Imprinting Org. Biomol. Chem.
松井淳
（学術雑誌、 2004 ）2, 2563-2566 2563 - 2566 2004.11
Single Work
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Composite of Au Nanoparticles and molecularly imprinted polymer as a sensing material Anal. Chem.
松井淳
（学術雑誌、 2004 ）76, 1310-1315 1310 - 1315 2004.11
Single Work
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Synthesis of Tripeptides Containing a Very Crowded a,a-Disubstituted Glycine with Pyridine Rings by Solid-Phase Ugi Reaction.
村嶋 貴之
Tetrahedron Lett, 2004.11
Single Work
Tetrahedron Lett, （学術雑誌、 2004 ）45, 8871-8874.
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A novel intramolecular hydrogen bonding between a side-chain pyridine ring and an amide hydrogen of the peptide backbone in tripeptides containing the new amino acid, a,a-di(2-pyridyl)glycine.
村嶋 貴之
Org. Biomol. Chem., 2004.11
Single Work
Org. Biomol. Chem., （学術雑誌、 2004 ） 2, 2335-2339 .
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Conformational change of a nucleotide by a base-pair mimic nucleoside in the complementary DNA strand
中野 修一
Nucleic Acids Symp. Ser. 48 259 - 260 2004.11
Single Work
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Secondary structure change of oligonucleotides induced by salts
中野 修一
Nucleic Acids Symp. Ser. 48 109 - 110 2004.11
Single Work
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Stabilization of a DNA duplex under molecular crowding conditions of PEG
中野 修一
Nucleic Acids Symp. Ser. 48 107 - 108 2004.11
Single Work
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Peptide Arrays with Designed Secondary Structures for Protein Characterization Using Fluorescent Fingerprint Patterns. Reviewed
臼井健二
Biopolymers ( 76 ) 129 - 139 2004.11
Single Work
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Stabilization of a DNA duplex under molecular crowding conditions of PEG （共著）
H. Karimata, S. Nakano, T. Ohmichi, J. Kawakami, and N. Sugimoto
Nucleic Acids Symp. Ser. 48 107 - 108 2004.11
Joint Work
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Analysis of the secondary structure of a ribozyme that requires His as a cofactor（共著）
J. Kawakami, C. Maiya, and N. Sugimoto
Nucleic Acids Symp. Ser. 48 203 - 204 2004.11
Joint Work
Authorship：Lead author
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The effect of molecular crowding with nucleotide length and cosolute structure on DNA duplex stability（共著） Reviewed
S. Nakano, H. Karimata, T. Ohmichi, J. Kawakami, and N. Sugimoto
Journal of American Chemical Society 126 14330 - 14331 2004.10
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"Spinnokinetic Analyses of Blood Disposition and Biliary Excretion of Nitric Oxide (NO)-Fe(II)-N-(Dithiocarboxy)sarcosine Complex in Rats: BCM-ESR and BEM-ESR Studies" Reviewed
Hiroyuki Yasui, Satoshi Fujii, Tetsuhiko Yoshimura, and Hiromu Sakurai
Free Radic. Res 38 ( 10 ) 1061 - 1072 2004.10
Joint Work
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Peptide arrays with designed alpha-helical structures for characterization of proteins from FRET fingerprint patterns Reviewed
Kenji Usui, Mizuki Takahashi, Kiyoshi Nokihara, Hisakazu Mihara
MOLECULAR DIVERSITY 8 ( 3 ) 209 - 218 2004.9
Publisher：SPRINGER
A practical high-throughput protein detection system is described, based on synthetic peptide arrays consisting of designed alpha-helical peptides, detected by fluorescence resonance energy transfer (FRET). Initially a model alpha-helical peptide known to interact with a structured protein, calmodulin, was selected to establish the strategy for high-throughput detection. In comparison to peptides with a single probe, a much higher FRET response has been observed with two fluorescent probes (7-diethylaminocoumarin-3-carboxylic acid and 5(6)-carboxy-fluorescein) at both termini of the synthetic peptides. To establish a reproducible high-throughput detection system, peptides were also immobilized onto a solid surface for detection of the target proteins. A small library of 112 different peptides was constructed, based on a model of the alpha-helical peptide with systematic replacement of residues carrying specific charges and/or hydrophobicities. The library was used to effectively characterize various proteins, giving their own 'protein fingerprint' patterns. The resulting 'protein fingerprints' correlate with the recognition properties of the proteins. The present microarray with designed synthetic peptides as the capturing agents is promising for the development of protein detection chips.
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Cycloprodigiosin up-regulates inducible nitric oxide synthase gene expression in hepatocytes stimulated by interleukin-1beta.
Teshima S, Nakanishi H, Kamata K, Kaibori M, Kwon AH, Kamiyama Y, Nishizawa M, Ito S, Okumura T
Nitric oxide : biology and chemistry 11 ( 1 ) 9 - 16 2004.8
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Influences of ribonucleotide on a duplex conformation and its thermal stability: Study with the chimeric RNA-DNA strands. Reviewed
S. Nakano, T. Kanzaki, and N. Sugimoto
J. Am. Chem. Soc. 126 1088 - 1095 2004.1
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Catalytic roles for proton transfer and protonation in ribozymes: Current understanding and future possibilities. Reviewed
P. C. Bevilacqua, T. S. Brown, S. Nakano, and R. Yajima
Biopolymers 73 90 - 109 2004.1
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Fluorescent in situ hybridization to ascidian chromosomes.
西方敬人
Zool. Sci. ( 21 ) 153 - 157 2004
Single Work
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A role of Ecdysteroid-phosphates during early embryonic development in silkworm Bombyx mori.
西方敬人
Mem. Konan Univ., Sci. Ser ( 51 ) 1 - 16 2004
Single Work
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CiYB1 is a major component of storage mRNPs in ascidian oocytes: implications in translational regulation of localized mRNAs
西方敬人
Developmental Biology ( 272 ) 217 - 230 2004
Single Work
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Synthesis, Surface Modification and Multilayer Construction of Mixed Monolayer-Protected CdS Nanoparticles
T. Tsuruoka, K. Akamatsu, H. Nawafune
Langmuir 20 11169 - 11174 2004
Joint Work
（学術雑誌、2004）3. 11169-11174
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Electroless Copper Plating Using Sn(II) Complex as a Reducing Agent for ULSI Metallization
H. Nawafune, Y. Kobata, K. Akamatsu, S. Mizumoto, Y. Murakami
Mem. Konan Univ., Sci. Eng.Ser. 51 29 - 38 2004
Joint Work
（大学・研究所紀要、2004）51, 29-38
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Preparation of transparent alumina film and fiber from a composite of aluminum polynuclear complex/methyl cellulose
Y. Gotoh, K. Fujimura, Y. Ohkoshi, M. Nagura, K. Akamatsu, and S. Deki
Mater. Chem. Phys. 83 54 - 59 2004
Joint Work
（学術雑誌、2004）83, 54-59
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Aqueous solution-based synthesis of rare earth-doped metal oxide thin films
S. Deki, K. Kuratani, M. Uemura, K. Akamatsu, M. Mizuhata, and A. Kajinami
Thin Solid Films 460 83 - 86 2004
Joint Work
（学術雑誌、2004）460, 83-86
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Polyaddition of 2,7-diethyl-9,9-dioctylfluorene using regio- and stereoselective alkyne dimerization catalysts
H. Katayama, M. Nakayama, T. Nakano, C. Wada, K. Akamatsu and F. Ozawa
Macromolecules 37 13 - 17 2004
Joint Work
（学術雑誌、2004）37, 13-17
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Composite of Au nanoparticles and molecularly imprinted polymer as a sensing material
J. Matsui, K. Akamatsu, S. Nishiguchi, D. Miyoshi, H. Nawafune, K. Tamaki and N. Sugimoto
Anal. Chem. 76 1310 - 1315 2004
Joint Work
（学術雑誌、2004）76, 1310-1315
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Direct Patterning of Copper on Polyimide Using Ion Exchangeable Surface Templates Generated by Site-Selective Surface Modification
K. Akamatsu, S. Ikeda, H. Nawafune, H. Yanagimoto
J. Am. Chem. Soc 126 10822 - 10823 2004
Joint Work
.（学術雑誌、2004）126, 10822-10823
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Formation and Growth of Copper Nanoparticles from Ion-Doped Precursor Polyimide Layers
S. Ikeda, K. Akamatsu, H. Nawafune, T. Nishino, S. Deki
J. Phys. Chem. B. 108 15599 - 15607 2004
Joint Work
（学術雑誌、2004）108, 15599-15607
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Development of a practical protein-chip using designed synthetic peptide-arrays Reviewed
K Nokihara, T Ohyama, K Usui, K Yonemura, K Tomizaki, H Mihara
KOBUNSHI RONBUNSHU 61 ( 10 ) 523 - 532 2004
Publisher：SOC POLYMER SCIENCE JAPAN
Novel high-throughput technologies, which can replace conventional electrophoresis and mass spectroscopic analyses, are in great demand for understanding the structure-function relationships of proteins. For a practical protein-detection system, arrays with immobilized designed synthetic peptides have been constructed. Peptides were labeled with fluorescent dyes in order to achieve high sensitivity. Two different types of prototype-arrayer have been constructed: one has a micro-dispensing system and the other a spot-printing system. Combinatorial peptide libraries, which consisted of beta-loop, beta-strand and alpha-helical peptides, were constructed by improved highly efficient solid-phase synthesis. Labeled peptides were covalently immobilized on solid supports such as precision glass plates. Various proteins were characterized with these peptide-arrays using a fluorescent scanner to give the "protein fingerprint" which is characteristic of the individual proteins. The results of the present prototype system demonstrate the practicality of protein-chips as a new generation of biochips.
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Peptide arrays with designed secondary structures for protein characterization using fluorescent fingerprint patterns Reviewed
K Usui, T Ojima, M Takahashi, K Nokihara, H Mihara
BIOPOLYMERS 76 ( 2 ) 129 - 139 2004
Publisher：JOHN WILEY & SONS INC
To realize a practical high-throughput protein-detection system, novel peptide arrays have been constructed using designed peptide libraries with loop, a-helix, or P-strand structures. Here, we describe the overview of the reported deigned peptide arrays with loop and a-helix structures and the new results of those with beta-strand structures. Initially, several model peptides known to interact with model structured proteins were selected to establish the present strategy for high-throughput detection of proteins. The fluorescent probes and suitable scaffolds of peptides were examined for the effective detection of proteins. The detection methods were established in solution and in an immobilized manner using the model systems. In the case of a-helix peptide, the response of a peptide with fluorescent resonance energy tran, fer between two probes at both termini was several times higher than that of a peptide with a single probe. In the cases of peptides with other structures, however, proteins were effectively detectable even by the fluorescent change of one probe. Furthermore, structurally focused libraries consisting of a total of ca. 250 different peptides based on the model pcptides with secondary and/or tertiary structures were constructed with systematic replacement of residues. Using these libraries, various proteins were characterized effectively to give their own fluorescent "protein fingerprint" patterns. The resulting protein fingerprints correlated with the recognition properties of the proteins. These studies demonstrate that arrays with peptide libraries based on designed structures can be promising tools for detecting the target proteins. Designed synthetic peptides play roles as the capturing agents to be developed for practical protein chips. (C) 2004 Wiley Periodicals, Inc.
DOI： 10.1002/bip.10568
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Development of Peptide-Chips Focusing on a High Throughput Protein-Detection System Reviewed
Kiyoshi Nokihara, Takafumi Ohyama, Kenji Usui, Koichi. Yoneyama, Mizuki Takahashi, Hisakazu Mihara
Innovation and Perspectives in Solid Phase Synthesis & Combinatorial Libraries 83 - 88 2004
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Peptide microarrays using structure-based peptide libraries for protein chips Reviewed
Kenji Usui, Mizuki Takahashi, Tetsunori Ojima, Masato Suzuki, Kiyoshi Nokihara, Eiichi Tamiya, Hisakazu Mihara
Peptide Revolution: Genomics, Proteomics & Therapeutics 258 - 259 2004
Joint Work
Publisher：AMER CHEMICAL SOC
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Synthesis, Surface Modification and Multilayer Construction of Mixed Monolayer-Protected CdS Nanoparticles Reviewed
T. Tsuruoka, K. Akamatsu, H. Nawafune
Langmuir 20 11169 - 11174 2004
Joint Work
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Microanalysis of Nucleic Acids Using the Limulus G Test
甲元一也
Anal. Sci ( 19 ) 1567 - 1568 2003.11
Single Work
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アンチセンス治療に向けた多糖系遺伝子キャリアー
甲元一也
月刊BIOINDUSTRY 20 ( 10 ) 30 - 37 2003.11
Single Work
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Polysaccharide-Polynucleotide Complexes Part 7. Hydrogen-ion and Salt Concentration Dependence of Complexation between Schizophyllan and Single-Stranded Homo RNAs
甲元一也
Bioorg. Chem ( 31 ) 216 - 226 2003.11
Single Work
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Supramolecular structure of a sugar-appended organogelator explored with synchrotron X-ray small angle scattering
甲元一也
Langmuir, ( 19 ) 8211 - 8217 2003.11
Single Work
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Polysaccharide-polynucleotide complexes Part 17. Solvent effects on conformational-transition of polydeoxyadenylic acid in the complex with schizophyllan
甲元一也
Polym. J. ( 35 ) 714 - 720 2003.11
Single Work
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Polysaccharide-polynucleotide Complexes (15): Thermal Stability of Schizophyllan(SPG)/Poly(C) Triple Strands is Controllable by the a-Amino Acid Modification
甲元一也
Bioorg. Chem ( 31 ) 163 - 171 2003.11
Single Work
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Structural Ttransition from Antiparallel to Parallel G-Quadruplex of d(G4T4G4) Induced by Ca2+.
三好大輔
Nucleic Acids Res ( 31 ) 1156 - 1163 2003.11
Single Work
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バイオインフォマティクスを活用した未来型生体内・外センシングシステム
三好大輔
科学と工業, ( 77 ) 413 - 419 2003.11
Single Work
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Novel biomaterials derived from deoxyribozyme and NAPzyme
三好大輔
Macromol. Chem. Phys. Macromol. Symp. ( 201 ) 245 - 252 2003.11
Single Work
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Effect of Putrescine and PEG on a Structural Transition of DNA G-Quadruplex.
三好大輔
Nucleosides, Nucleotides, and Nucleic Acids ( 22 ) 1591 - 1594 2003.11
Single Work
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Structural Competition Involving G-Quadruplex DNA and Its Complement.
三好大輔
Biochemistry ( 42 ) 11736 - 11744 2003.11
Single Work
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Structural Polymorphism of Telomeric DNA Regulated by pH and Divalent Cation.
三好大輔
Nucleosides, Nucleotides, and Nucleic Acids ( 22 ) 203 - 221 2003.11
Single Work
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Facile One-Pot Synthesis of Aromatic and Heteroaromatic Sulfonamides.
村嶋 貴之
J. Org. Chem., 2003.11
Single Work
J. Org. Chem., （学術雑誌、 2003）68, 8274-8276.
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A new synthesis of functional dyes from 2-acenaphtho[1,2-c]pyrrole.
村嶋 貴之
Heterocycles, 2003.11
Single Work
Heterocycles, （学術雑誌、 2003） 61, 433-447 .
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Utilization of proteases from Aspergillus species for peptide synthesis via the kinetically controlled approach.
村嶋 貴之
Biocatal. Biotrans., 2003.11
Single Work
Biocatal. Biotrans., （学術雑誌、 2003） 21, 93-100 .
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Novel biomaterials derived from deoxyribozyme and napzyme.
中野 修一
Macromol. Symp. 201 245 - 252 2003.11
Single Work
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An oligopeptide containing the C-terminal sequence of RNase A inhibits an RNA hydrolysis by RNase A.
S. Nakano, A. L. Cerrone, and P. C. Bevilacqua
First International Symposium on Biomolecular Chemistry 240 - 241 2003.11
Single Work
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Large stacking stability of a base pair-mimic nucleotide on the DNA duplex.
中野 修一
Nucleic Acids Res. Supple. 3 79 - 80 2003.11
Single Work
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Negative regulation of Lyn protein-tyrosine kinase by c-Cbl ubiquitin-protein ligase in Fc epsilon RI-mediated mast cell activation.
Kyo S, Sada K, Qu X, Maeno K, Miah SM, Kawauchi-Kamata K, Yamamura H
Genes to cells : devoted to molecular & cellular mechanisms 8 ( 10 ) 825 - 836 2003.10
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Structural competition involving G-quadruplex DNA and its complement. Reviewed
W. Li, D. Miyoshi, S. Nakano, and N. Sugimoto
Biochemistry, 42 11736 - 11744 2003.9
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“Pentacoordinated iron complexes with carboxamido nitrogens and thiolate sulfurs: As models for Fe-containing nitrile hydratase”
Satoshi Fujii, Takeshi Ogura, Shozo Kitamura and Hiroshi Sakai
J. Inorg. Biochem 96 134 2003.7
Joint Work
Authorship：Lead author
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Adaptor protein 3BP2 is a potential ligand of Src homology 2 and 3 domains of Lyn protein-tyrosine kinase.
Maeno K, Sada K, Kyo S, Miah SM, Kawauchi-Kamata K, Qu X, Shi Y, Yamamura H
The Journal of biological chemistry 278 ( 27 ) 24912 - 24920 2003.7
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An Oligopeptide containing the C-terminal sequence of RNase A has a potent RNase A binding property. Reviewed
S. Nakano and N. Sugimoto
J. Am. Chem. Soc. 125 8728 - 8729 2003.6
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Large stabilization of a DNA duplex by the deoxyadenosine derivatives tethering an aromatic hydrocarbon group. Reviewed
S. Nakano, Y. Uotani, S. Nakashima, Y. Anno, M. Fujii, and N. Sugimoto
J. Am. Chem. Soc. 125 8086 - 8087 2003.6
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核酸の長鎖ダングリングエンドと四重鎖の構造形成に関する熱力学的研究
三好大輔
2003.3
Single Work
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Mechanistic chracterization of the HDV genomic ribozyme: Classifying the catalitic and structural metal ion sites within a multichannel mechanism. Reviewed
中野 修一
Biochemistry 42 2982 - 2994 2003.2
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Site-selective direct silver metallization on surface-modified polyimide layers
K. Akamatsu, S. Ikeda, H. Nawafune
Langmuir 19 10366 - 10371 2003
Joint Work
（学術雑誌、2003）19, 10366-10371
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低温鉛フリーはんだ接合を目的としたスズー亜鉛共晶合金の電析および亜鉛の共析機構
縄舟秀美、中谷敏雄、赤松謙祐、内田衛、小幡恵吾
エレクトロニクス実装学会誌 6 222 - 227 2003
Joint Work
（学術雑誌、2003）6, 222-227
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Fabrication and structural characterization of nanocomposites consisting of Ni nanoparticles dispersed in polyimide films
K. Akamatsu, K. Nakahashi, S. Ikeda, H. Nawafune
Eur. Phys. J. D 24 377 - 380 2003
Joint Work
（学術雑誌、2003）24, 377-380
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Fabrication of copper film on aluminum nitride by novel electroless plating
H. Yanagimoto, K. Akamatsu, S. Deki, K. Gotoh
J. Electrochem. Soc. 150 260 - 265 2003
Joint Work
（学術雑誌、2003）150, 260-265
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Covalent bond-mediated network fabrication of carboxylic anhydride-protected gold nanoparticles
K. Akamatsu, J. Hasegawa, H. Nawafune, H. Katayama, F. Ozawa
Trans. Mater. Res. Soc. Jpn. 28 927 - 930 2003
Joint Work
（学術雑誌、2003）28, 927-930
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ULSI銅微細配線の形成を目的とした中性無電解銅めっきにおけるビアフィリング特性の改善
縄舟秀美、樋口晋吾、赤松謙祐、内田衛
表面技術 54 683 - 688 2003
Joint Work
（学術雑誌、2003）54, 683-688
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Preparation and characterization of Au/Co nano-alloys
H. Nabika, M. Mizuhata, K. Kajinami, S. Deki, K. Akamatsu
J. Electroanal. Chem. 559 99 - 102 2003
Joint Work
（学術雑誌、2003）559, 99-102
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Introduction of Ag and Ag2S nanoparticles into nylon 6 film and fiber
Y. Gotoh, T. Kanno, Y. Fujimori, Y. Ohkoshi, M. Nagura, K. Akamatsu, S. Deki
Polymer Journal 35 960 - 964 2003
Joint Work
（学術雑誌、2003）35, 960-964
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Synthesis of metallized polyimide films by surface chemical modification
S. Ikeda, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn 28 1267 - 1270 2003
Joint Work
.（学術雑誌、2003）28, 1267-1270
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Ring-opening methathesis polymerization using alkenyl sulfides as chain-transfer agents: efficient routes to unsymmetrical poly(norbornen)-based macroinitiators bearing a terminal hydroxy group
H. Katayama, Y. Fukuse, Y. Nobuto, K. Akamatsu, F. Ozawa
Macromolecules 36 7020 - 7026 2003
Joint Work
（学術雑誌、2003）36, 7020-7026
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NH2-terminated poly(ethylene oxide) containing nano-sized NiO particles: synthesis, characterization and structural considerations
S. Deki, H. Yanagimoto, S. Hiraoka, K. Akamatsu, K. Gotoh
Chem. Mater. 15 4916 - 4922 2003
Joint Work
（学術雑誌、2003）15, 4916-4922
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Surface modification-based synthesis and microstructural tuning of nanocomposite layers: monodispersed copper nanoparticles in polyimide resins
K. Akamatsu, S. Ikeda, H. Nawafune, S. Deki
Chem. Mater. 15 1488 - 2491 2003
Joint Work
（学術雑誌、2003）15, 1488-2491
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Novel biomaterials derived from deoxyribozyme and NAPzyme Reviewed
Y. Okumoto, H. Fujiki, J. Kawakami, S. Nakashima, S. Nakano, T. Ohmichi, D. Miyoshi and N. Sugimoto
Macromol. Chem. Phys., Macromol. Symp. 201 245 - 252 2003
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Important effects of non-Watson-Crick interactions and cation binding on nucleic acid structures.
中野 修一
Recent research development in nucleosides＆nucleotides （Transworld Research Networks）, Vol. 1, 59-80 2003
Single Work
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Temparature dependence of themodynamic properties for DNA/DNA and RNA/DNA duplex formation. Reviewed
P. Wu, S. Nakano, and N. Sugimoto
Eur. J. Biochem. 269 2821 - 2830 2002.11
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Molecular Design of “Super” Hydrogelators: Understanding the Gelation process of Azobenzene-Based Sugar Derivatives in Water
甲元一也
Org. Lett., ( 4 ) 1423 - 1426 2002.11
Single Work
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Chemical modification of curdlan: Characteristic complexation with polynucleotides by control of the molecular weight
甲元一也
Polym. Prep 43 ( 2 ) 721 - 723 2002.11
Single Work
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Novel DNA-polysaccharide triple helix and its application to a gene carrier
甲元一也
Polym. Prep 43 ( 2 ) 691 - 692 2002.11
Single Work
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Polysaccharide-polynucleotide Interaction (XI); Novel Separation System of RNAs by Using Schizophyllan-appended Column
甲元一也
Chem. Lett. 1240 - 1241 2002.11
Single Work
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Polysaccharide-Polynucleotide Complexes Part 12: Enhanced affinity for various polynucleotide chains by site-specific chemical modification of schizophyllan
甲元一也
Perkin Trans. 1 2477 - 2484 2002.11
Single Work
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Sol-gel phase transition induced by fiber-vesicle structural changes in sugar-based bolaamphiphiles
甲元一也
Perkin Trans 2 1930 - 1936 2002.11
Single Work
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Gel formation properties of a uracil-appended cholesterol gelator and cooperative effects of the complementary nucleobases
甲元一也
Tetrahedron ( 58 ) 8863 - 8873 2002.11
Single Work
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Polysaccharide-Polynucleotide Complexes Part 8. Cation Induced Complex Formation between Polyuridylic Acid and Schizophyllan
甲元一也
Biopolymers ( 65 ) 1 - 9 2002.11
Single Work
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Long RNA Dangling End Has Large Energetic Contribution to Duplex Stability.
三好大輔
J. Am. Chem. Soc. ( 124 ) 10367 - 10372 2002.11
Single Work
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シリーズ「生命を化学する」—モレキュラークラウディングとin cell Chemistry—
三好大輔
化学（化学同人） 57 ( 12 ) 50 - 56 2002.11
Single Work
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超タンパク質材料—タンパク質活用最前線—
三好大輔
未来材料（NTS） ( 5 ) 29 - 35 2002.11
Single Work
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Molecular Crowding Regulates the Structural Switch of the DNA G-quadruplex.
三好大輔
Biochemistry, ( 41 ) 15017 - 15024 2002.11
Single Work
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Molecular Imprinting in Alcohols: utility of a pre-polymer based strategy for synthesizing stereoselective artificial receptor polymers in media hydrophilic Anal.Chim.Acta
松井淳
（学術雑誌、 2002 ） 466, 11-15 11 - 15 2002.11
Single Work
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コンビナトリアル・モレキュラーインプリンティングで目指す水系での分子認識
松井淳
化学（学術雑誌、 2002 ）57, 33-38 33 - 38 2002.11
Single Work
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Preparation of Novel Heteroisoindoles from Nitro Pyridines and Nitro Pyridones.
村嶋 貴之
Heterocycles, 2002.11
Single Work
Heterocycles, （学術雑誌、 2002） 58, 301-310 .
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Aspergillus melleus protease-catalyzed peptide synthesis using the carbamoylmethyl ester as an acyl donor in 1,1,1,3,3,3-hexafluoro-2-propanol/N,N-dimethylformamide.
村嶋 貴之
Biotechnol. Lett., 2002.11
Single Work
Biotechnol. Lett., （学術雑誌、 2002） 24, 1945-1949.
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Bacillus licheniformis protease-catalyzed peptides synthesis via the kinetically controlled approach using the carbamoylmethyl ester as an acyl donor in anhydrous acetonitrile.
村嶋 貴之
Lett. Pept. Sci., 2002.11
Single Work
Lett. Pept. Sci., （学術雑誌、 2002） 9, 173-177 .
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Role of 2'-OH on Thermodynamic Stability and Structure of Chimeric Oligonucleotides
中野 修一
Nucleic Acids Res. Supple. 2 189 - 190 2002.11
Single Work
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Long RNA Dangling end Has Large Energetic Contribution to Duplex Stability. Reviewed
T. Ohmichi, S. Nakano, D. Miyoshi, and N. Sugimoto
J. Am. Chem. Soc. 124 10367 - 10372 2002.8
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Hydrogen peroxide activates IkappaB kinases through phosphorylation of serine residues in the activation loops.
Kamata H, Manabe T, Oka Si, Kamata K, Hirata H
FEBS letters 519 ( 1-3 ) 231 - 237 2002.5
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“Comparison of Nitric Oxide Production in Response to Carbachol Between Macrovascular and Microvascular Cardiac Endothelial Cells” Reviewed
Takehiko Miyashita, Yasuchika Takeishi, Hiroki Takahashi, Takuya Miyamoto, Satoshi Fujii, Tetsuhiko Yoshimura, Hitonobu Tomoike, Shuichi Kato, and Isao Kubota
Circulation Journal, 66 ( 5 ) 511 - 515 2002.4
Joint Work
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「アルツハイマー病とNO・金属イオンの化学」
藤井 敏司,杉本直己
化学 57 ( 4 ) 43 - 47 2002.4
Joint Work
Authorship：Lead author
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「狂牛病・プリオン病と金属イオンの化学」
藤井 敏司,杉本直己
化学 57 ( 3 ) 30 - 34 2002.3
Joint Work
Authorship：Lead author
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"In Vivo Nitric Oxide Transfer of a Physiological NO Carrier, Dinitrosyl Dithiolato Iron Complex, to Target Complex" Reviewed
Takaharu Ueno, Yasuhiro Suzuki, Satoshi Fujii, Anatoly F. Vanin, and Tetsuhiko Yoshimura
Biochem. Pharmacol. 63 ( 3 ) 485 - 493 2002.2
Joint Work
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Ciona intestinalis cDNA projects: expressed sequence tag analyses and gene expression profiles during embryogenesis
西方敬人
Gene ( 287 ) 83 - 96 2002
Single Work
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Novel G-protein-coupled receptor gene expressed specifically in the entire neural tube of the ascidian Ciona intestinalis
西方敬人
Development, Genes and Evolution ( 212 ) 447 - 451 2002
Single Work
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Highly reactive intermediate-functionalized gold clusters: Synthesis and immobilization on silica supports through amide-forming coupling
K. Akamatsu, J. Hasegawa, H. Nawafune, H. Katayama, F. Ozawa
J. Mater. Chem. 12 2862 - 2865 2002
Joint Work
（学術雑誌、2002）12, 2862-2865
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鉛フリーはんだ接合を目的としたスルホコハク酸浴からのSn-0.7Cu-0.3Ag３元合金の電析
縄舟秀美、芝一博、赤松謙祐、水本省三、内田衛、小幡恵吾
エレクトロニクス実装学会誌 5 146 - 151 2002
Joint Work
（学術雑誌、2002）5, 146-151
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フリップチップ実装を目的としたスルホコハク酸浴からのスズ−インジウム共晶合金の電析
縄舟秀美、中谷敏雄、赤松謙祐、水本省三、小幡恵吾、内田衛
エレクトロニクス実装学会誌 5 349 - 352 2002
Joint Work
（学術雑誌、2002）5, 349-352
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Fabrication and characterization of PAN-derived carbon thin films containing Au nanoparticles
S. Deki, H. Nabika, K. Akamatsu, M. Mizuhata, A. Kajinami, S. Tomita, M. Fujii, S. Hayashi
Thin Solid Films 408 59 - 63 2002
Joint Work
（学術雑誌、2002）408, 59-63
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Novel copper electroless plating process on ceramic substrate using copper oxide
H. Yanagimoto, K. Akamatsu, S. Deki, K. Gotoh
Electrochem. Solid State Lett. 5 C87 - C89 2002
Joint Work
（学術雑誌、2002）5, C87-C89
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Growth of metal oxide thin films from aqueous solution by liquid phase deposition, LPD, method
S. Deki, N. Yoshida, Y. Hiroe, K. Akamatsu, M. Mizuhata, A. Kajinami
Solid State Ionics 151 1 - 9 2002
Joint Work
（学術雑誌、2002）151, 1-9
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Microstructure and electron transport properties of AuxCo1-x nano-alloys embedded in polyacrylonitrile thin films
H. Nabika, K. Akamatsu, M. Mizuhata, A. Kajinami, S. Deki
J. Mater. Chem. 12 2408 - 2411 2002
Joint Work
（学術雑誌、2002）12, 2408-2411
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Effect of composition and structure of gold/copper bimetallic nanoparticles on dispersion in polymer thin films
K. Akamatsu, T. Kawamura, H. Nabika, S. Deki, T. Strunskus, F. Faupel
J. Mater. Chem. 12 3610 - 3614 2002
Joint Work
（学術雑誌、2002）12, 3610-3614
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Synergistic effects induced by cycloprodigiosin hydrochloride and epirubicin on human breast cancer cellsYamamoto D Reviewed
Tanaka K, Nakai K, Baden T, Inoue K, Yamamoto C, Takemoto H, Kamata K, Hirata H, Morikawa S, Inubushi T, Hioki K
Breast Cancer Research and Treatment 72 2002
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分子転写法による新素材の開発 –生体分子を超える分子認識材料開発を目指して-
甲元一也
未来材料 1 ( 5 ) 35 - 41 2001.11
Single Work
-
Application of schizophyllan as a novel gene carrier
甲元一也
Nucleic Acids Res. Supplement ( 1 ) 223 - 224 2001.11
Single Work
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A novel technique to specifically separate RNAs by Schizophyllan
甲元一也
Nucleic Acids Res. Supplement ( 1 ) 283 - 284 2001.11
Single Work
-
Chemical Modification of Curdlan to Induce an Interaction with Poly(C)
甲元一也
Chem. Lett 908 - 909 2001.11
Single Work
-
Chemical modification of schizophyllan by introduction of a cationic charge into the side chain which enhances the thermal stability of schizophyllan/poly(C) complexes
甲元一也
Chem. Commun 1962 - 1963 2001.11
Single Work
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Organization of Nucleosides Supported by Boronic-Acid-Appended poly(L-Lysine): Creation of a Novel RNA Mimic
甲元一也
Bull. Chem. Soc. Jpn ( 74 ) 1311 - 1317 2001.11
Single Work
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Polysaccharide-Polynucleotide Complexes (IV): Anti-hydrolysis effect of the schizophyllan/poly(C) complex and the complex dissociation induced by amines
甲元一也
Bioorg. Chem., ( 29 ) 178 - 185 2001.11
Single Work
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Thermally induced conformational–transition of polydeoxyadenosine in the complex with schizophyllan and the base-length dependence of its stability
甲元一也
Chem. Commun. 429 - 430 2001.11
Single Work
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A Stable DNA tetraloop and Its Structural Tolerance for Modification.
三好大輔
Chem. Lett 258 - 259 2001.11
Single Work
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Structural transition of d(G4T4G4) from antiparallel to parallel G-quartet induced by divalent cations
三好大輔
Nucleic Acids Res. Supple ( 1 ) 259 - 260 2001.11
Single Work
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Effect of Divalent Cations on Antiparallel G-Quartet Structure of d(G4T4G4).
三好大輔
FEBS Lett ( 496 ) 128 - 133 2001.11
Single Work
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Molecularly Imprinted Polymers with Metalloporphyrin-Based Molecular Recognition Sites Coassembled with Methacrylic Acid Anal.Chem.
松井淳
（学術雑誌、 2001 ） 73, 3869-3874 3869 - 3874 2001.11
Single Work
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Molecularly Imprinted Polymer Library on a Microtiter Plate: High-throughput Synthesis and Assessment of Cinchona Alkaloid-imprinted Polymer Receptors Instrumentation Sci. & Technologyvol.
松井淳
（学術雑誌、2001）29, 1-9 1 - 9 2001.11
Single Work
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Combinatorial Molecular Imprinting for Formation of Atrazine Decomposing Polymers Chem.Lett.
松井淳
（学術雑誌、 2001 ） 530-531 530 - 531 2001.11
Single Work
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Synthesis and optical properties of a new class of pyrromethene-BF2-complexes fised with rigid bicyclo rings and benzo derivatives.
村嶋 貴之
Tetrahedron Lett., 2001.11
Single Work
Tetrahedron Lett., （学術雑誌、 2001） 6711-6713.
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Synthesis of benzoporphyrins functionalized with octaester groups.
村嶋 貴之
Tetrahedron Lett., 2001.11
Single Work
Tetrahedron Lett.,（学術雑誌、2001） 42, 45-47.
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Synthesis and properties of polypyrrole annelated with bicyclo[2.2.2]octene units.
村嶋 貴之
Tetrahedron Lett., 2001.11
Single Work
Tetrahedron Lett.,（学術雑誌、2001） 42, 707-710.
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Preparation of meso-unsubstituted porphyrins substituted with mono- and tetraformyl and other electron-withdrawing groups.
村嶋 貴之
Heterocycles, 2001.11
Single Work
Heterocycles,（学術雑誌、2001） 54, 705-720.
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Synthesis of b,b'-(phenylene)diporphyrins.
村嶋 貴之
Synthesis, 2001.11
Single Work
Synthesis, （学術雑誌、2001） 399-402.
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Synthesis of oligo(thienylenevinylenes) substituted with alkoxy groups.
村嶋 貴之
Heteroatom Chemistry, 2001.11
Single Work
Heteroatom Chemistry, （学術雑誌、 2001） 12, 414-417.
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“Spin trapping agent, phenyl N-tert-butylnitrone, reduces nitric oxide production in the rat brain during experimental meningitis”
藤井 敏司
Free Radic Res ( 35 ) 583 - 591 2001.11
Single Work
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Proton Inventory of the Genomic HDV Ribozyme in Mg2+-Containing Solutions. Reviewed
S. Nakano and P. C. Bevilacqua
J. Am. Chem. Soc. 123 11333 - 11334 2001.10
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Mechanistic Characterization of the HDV Genomic Ribozyme: Assessing the Catalytic and Structural Contributions of Divalent Metal Ions within a Multichannel Reaction Mechanism. Reviewed
S. Nakano, D. J. Proctor, and P. C. Bevilacqua
Biochemistry 40 12022 - 12038 2001.9
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Stabilization Factors Affecting Duplex Formation fo Peptide Nucleic Acid with DNA. Reviewed
N. Sugimoto, N. Satoh, K. Yasuda, and S. Nakano
Biochemistry 40 8444 - 8451 2001.6
-
A Stable DNA Tetraloop and Its Structural Tolerance for Modification
KAWAKAMI Junji, YONEYAMA Mari, MIYOSHI Daisuke, SUGIMOTO Naoki
Chemistry Letters 2001 ( 3 ) 258 - 259 2001.3
Publisher：The Chemical Society of Japan
Structural features of a stable hairpin DNA with a tetraloop, dAGGC(TTCG)GCCT (CORE), and its six abasic mutants were investigated by the melting behavior of the UV absorption and circular dichroism spectra. The CORE and an abasic mutant at the second position of the loop exhibit considerable thermodynamic stability and have similar structural features suggesting the same folding with an identical base orientation of the two hairpin DNAs except for the second base in the loop.
DOI： 10.1246/cl.2001.258
-
Maternal genetic information stored in fertilized eggs of the ascidian, Halocynthia roretzi.
西方敬人
in Biology of Ascidian Springer-Verlag 165 - 177 2001
Single Work
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Profiles of maternally expressed genes in fertilized eggs of Ciona intestinalis
西方敬人
Developmental Biology ( 238 ) 315 - 331 2001
Single Work
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Large-scale cDNA analysis of the maternal genetic information in the egg of Halocynthia roretzi for a gene expression catalog of ascidian development.
西方敬人
Development ( 128 ) 2555 - 2567 2001
Single Work
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Utilization of ribozymes for loss-of-function analyses of the early development of the ascidian Ciona intestinalis.
西方敬人
Mem. Konan Univ., Sci. Ser. ( 48 ) 11 - 20 2001
Single Work
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Functional analysis of Ciona intestinalis Y-box protein.
西方敬人
in Biology of Ascidian Springer-Verlag 186 - 192 2001
Single Work
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RNA-binding proteins in ascidian development.
西方敬人
RNA-binding proteins in ascidian development. 178 - 185 2001
Single Work
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Utilization of ribozymes for loss-of-function analyses of the early development of the ascidian Ciona intestinalis.
Katano, M., Yamada, A., Tanaka, K. J., Murakami, A., Taira, K., Kawakami, J. Sugimoto, N., and Nishikata, T.
Mem. Konan Univ. Sci. Ser. 48 11 - 20 2001
Single Work
-
Improved antigen detection on Western-blots Reviewed
Matsugu, H., Nishikata, T.
Technical Tips Online 6 ( 1 ) 34 - 36 2001
-
Direct photochemical formation of Cu patterns on surface modified polyimide resin
S. Ikeda, K. Akamatsu, H. Nawafune
J. Mater. Chem. 11 2919 - 1921 2001
Joint Work
（学術雑誌、2001）11, 2919-1921
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Formation of copper nanoparticles in surface modified polyimide resin
K. Akamatsu, S. Ikeda, H. Nawafune, S. Dek
Proc. of the 7th International Symposium on Advanced Physical Fields 2001 392 - 395 2001
Joint Work
（学術雑誌、2001）, 392-395
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Single-electron tunneling effects in nylon 11 thin films containing nanoparticles
M. Fujii, Y. Inoue, S. Hayashi, K. Akamatsu, S. Deki
Jpn. J. Appl. Phys. 40 1911 - 1914 2001
Joint Work
（学術雑誌、2001）40, 1911-1914
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Preparation and characterization of metal nanoparticles dispersed in polyacrylonitrile thin film
S. Deki. H. Nabika, K. Akamatsu, M. Mizuhata, A. Kajinami
Scripta Mater. 44 1879 - 1882 2001
Joint Work
（学術雑誌、2001）44, 1879-1882
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Formation process and characterization of copper oxide nanoparticles dispersed in polymer thin films
K. Akamatsu, S. Deki
Scripta Mater. 44 2149 - 2152 2001
Joint Work
（学術雑誌、2001）44, 2149-2152
-
ポリイミド樹脂の表面改質および熱圧着を利用する銅のDirect Metallization
池田慎吾、赤松謙祐、縄舟秀美、水本省三、清田優
エレクトロニクス実装学会誌 4 603 - 606 2001
Joint Work
（学術雑誌、2001）4, 603-606
-
添加剤の予備吸着を利用する銅電析プロセスによるULSI微細配線の形成
縄舟秀美、粟野まいこ、赤松謙祐、水本省三、内田衛、細田勉
表面技術 53 59 - 64 2001
Joint Work
（学術雑誌、2001）53, 59-64
-
Synthesis of titanium carbide from a composite of TiO2 nanoparticles/methyl cellulose by carbothermal reduction
Y. Gotoh, K. Fujimura, M. Koike, Y. Ohkoshi, M. Nagura, K. Akamatsu, S. Deki
Mater. Res. Bull. 36 2263 - 2275 2001
Joint Work
（学術雑誌、2001）36, 2263-2275
-
Preparation of Cu2O nanoparticles dispersed in NH2-terminated polyethylene oxide matrix
H. Yanagimoto, K. Akamatsu, K. Goto, S. Deki
Eur. Phys. J. D 16 313 - 316 2001
Joint Work
（学術雑誌、2001）16, 313-316
-
Synthesis and microstructure of metal oxide thin films containing metal nanoparticles by liquid phase deposition (LPD) method
S. Deki, Hin Yu Yu Ko, T. Fujita, K. Akamatsu, M. Mizuhata, A. Kajinami
Eur. Phys. J. D 16 325 - 328 2001
Joint Work
（学術雑誌、2001）16, 325-328
-
Composition dependence on dispersion of Au/Cu bimetallic nanoparticles in nylon 11 thin films
K. Akamatsu, T. Kawamura, S. Deki
Eur. Phys. J. D 16 305 - 308 2001
Joint Work
（学術雑誌、2001）16, 325-328
-
Novel fabrication method for Si1-xTixO2 thin films with garaded composition profiles by liquid phase deposition
S. Deki, S. Iizuka, K. Akamatsu, M. Mizuhata, A. Kajinami
J. Mater. Chem. 11 984 - 986 2001
Joint Work
（学術雑誌、2001）11, 984-986
-
Synthesis and characterization of Cu2O nanoparticles dispersed in NH2-terminated poly(ethylene oxide)
H. Yanagimoto, K. Akamatsu, K. Gotoh, S. Deki
J. Mater. Chem. 11 2387 - 2389 2001
Joint Work
（学術雑誌、2001）11, 2387-2389
-
Utilization of ribozymes for loss-of-function analyses of the early development of the ascidian Ciona intestinalis
M. Katano, A. Yamada, K. J. Tanaka, A. Murakami, K. Taira, J. Kawakami, N. Sugimoto and T. Nishikata
Mem. Konan Univ., Sci. Eng. Ser. 48 11 - 20 2001
-
Thermodynamic stability of base pairs between 2-hydroxyadenine and incoming nucleotides as a determinant of nucleotide incorporation specificity during replication Reviewed
J. Kawakami, H. Kamiya, K. Yasuda, H. Fujiki, H. Kasai and N. Sugimoto
Nucleic Acids Research 29 3289 - 3296 2001
-
Cycloprodigiosin hydrocloride suppresses tumor necrosis factor (TNF) alpha-induced transcriptional activation by NF-kappaB Reviewed
Kamata K, Okamoto S, Oka S, Kamata H, Yagisawa H, Hirata H
FEBS Letters 507 2001
Authorship：Lead author
-
Cycloprodigiosin hydrochloride, a H+/Cl- symporter, induces apoptosis in human colon cancer cell lines in vitro
Yamamoto C, Takemoto H, Kuno K, Yamamoto D, Nakai K, Baden T, Kamata K, Hirata H, Watanabe T, Inoue K
Oncology Reports 8 2001
-
Colorimetric Sugar Sensing Method Useful in “Neutral” Aqueous Media
甲元一也
Chem. Lett., 856 - 857 2000.11
Single Work
-
TEM and SEM observations of super-structures constructed in organogel systems from a combination of boronic-acid-appended bola-amphiphiles with chiral diols
甲元一也
Nanotechnology, ( 12 ) 25 - 31 2000.11
Single Work
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Polysaccharide-Polynucleotide Complex (III): A novel interaction between the b-1,3-Glucan Family and the Single-Strand RNA: Poly(C)
甲元一也
Chem. Lett 1242 - 1243 2000.11
Single Work
-
The First Example of Positive Allosterism in an Aqueous Saccharide-Binding System Designed on a Ce(IV) Bis(porphyrinate) Double Decker Scaffold
甲元一也
Tetrahedron ( 56 ) 4717 - 4723 2000.11
Single Work
-
Development of Small Peptides Recognizing a Monosaccharide by Combinatorial Chemistry.
三好大輔
Chem. Commun 2295 - 2296 2000.11
Single Work
-
ゲノム化学は今3 SNPs解析と次世代の生命化学 —「ニューセントラルドグマ」の構築に向けて.
三好大輔
化学（化学同人） ( 55 ) 20 - 25 2000.11
Single Work
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ゲノム化学は今2 DNAチップとバイオインフォマティクス —遺伝子の定量的解析に向けて.
三好大輔
化学（化学同人） ( 55 ) 14 - 18 2000.11
Single Work
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Molecularly Imprinted 9-Ethyladenine Receptor Having Porphyrin-based Recognition Center J. Am. Chem. Soc
松井淳
.（学術雑誌、2000）122, 5218-5219 5218 - 5219 2000.11
Single Work
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Solid - phase Extraction with a Dibutylmelamine-imprinted Polymer as Triazine Herbicide - selective Sorbent
松井淳
J. Chromatogr., A（学術雑誌、2000）889, 25-31 25 - 31 2000.11
Single Work
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Miniaturized Molecularly Imprinted Contiunous Polymer Rods J. High Resolution Chromatogr.
松井淳
J. High Resolution Chromatogr.（学術雑誌、2000）23, 44-46 44 - 46 2000.11
Single Work
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Molecularly Imprinted Fluorescent-shift Receptors Prepared Using 2-(Trifluoromethyl)acrylic Acid Anal. Chem.
松井淳
（学術雑誌、2000）72, 3286-3290 3286 - 3290 2000.11
Single Work
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Atrazine-selective Polymers Prepared by Molecular Imprinting of Trialkylmelamines as Dummy Template Species of Atrazine Anal. Chem.
松井淳
（学術雑誌、2000）72, 1810-1813 1810 - 1813 2000.11
Single Work
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Synthesis and coplanarity-dependent HOMO-LUMO separation of conjugated dimers.
村嶋 貴之
J. Chem. Soc., Perkin Trans. 1, 2000.11
Single Work
J. Chem. Soc., Perkin Trans. 1,（学術雑誌、2000） 2671-2675.
-
A new synthesis of benzoporphyrins using 4,7-dihydro-4,7-ethano-2H-isoindole as an isoindole equivalent.
村嶋 貴之
Heterocycles, 2000.11
Single Work
Heterocycles,（学術雑誌、2000） 52, 399-411.
-
A new synthesis of [2,3]naphthoporphyrins.
村嶋 貴之
Chem. Commun., 2000.11
Single Work
Chem. Commun.,（学術雑誌、2000） 893-894.
-
Synthesis and structures of pyrroles fused with rigid bicyclic ring systems at b-positions.
村嶋 貴之
J. Chem. Soc., Perkin Trans. 1, 2000.11
Single Work
J. Chem. Soc., Perkin Trans. 1,（学術雑誌、2000） 4347-4355.
-
Synthesis and X䀭ray structure of stable 2H-isoindoles.
村嶋 貴之
J. Chem. Soc., Perkin Trans. 1 2000.11
Single Work
J. Chem. Soc., Perkin Trans. 1,（学術雑誌、2000） 995-998.
-
“Nitric Oxide Enhances Expression and Shedding of Tumor Necrosis Factor Receptor I (p55) in Endothelial Cells”
藤井 敏司
Arterioscler Thromb Vasc Biol. ( 20 ) 1506 - 1511 2000.11
Single Work
-
“Detection and Imaging of Endogenously Produced Nitric Oxide with Electron Paramagnetic Resonance Spectroscopy”
藤井 敏司
Antiox. Redox Signal ( 2 ) 879 - 901 2000.11
Single Work
-
"A New Trend in Iron-Dithiocarbamate Complexes: As an Endogenous NO Trapping Agent."
藤井 敏司
Coord. Chem. Rev ( 198 ) 89 - 99 2000.11
Single Work
-
"Reaction of Nitric Oxide with the Iron(III) Complex of N-(dithiocarboxy)sarcosine: A New Type of Reductive Nitrosylation Involving Iron(IV) as an Intermediate”
藤井 敏司
J. Chem. Soc. Dalton Trans 3310 - 3315 2000.11
Single Work
-
Thermodynamics-Structure Relationship of Single Mismatches in RNA/DNA Duplexes. Reviewed
N. Sugimoto, M. Nakano, and S. Nakano
Biochemistry 39 11270 - 11281 2000.8
-
A Role for Upstream RNA Structure in Facilitating the Catalytic Fold of the Genomic Hepatitis Delta Virus Ribozyme. Reviewed
D. M. Chadalavada, S. M. Knudsen, S. Nakano, and P. C. Bevilacqua
J. Mol. Biol. 301 349 - 367 2000.3
-
Expression patterns of musashi homologs of the ascidians, Halocynthia roretzi and Ciona intestinalis Reviewed
T Kawashima, AR Murakami, M Ogasawara, KJ Tanaka, R Isoda, Y Sasakura, T Nishikata, H Okano, KW Makabe
DEVELOPMENT GENES AND EVOLUTION 210 ( 3 ) 162 - 165 2000.3
Joint Work
Publisher：SPRINGER VERLAG
The gene family encoding RNA-binding proteins includes important regulators involved in the neurogenesis in both protostomes and deuterostomes. We isolated cDNAs of the ascidian homolog of one of the RNA-binding proteins, MUSASHI, from Halocynthia roretzi and Ciona intestinalis. The predicted amino acid sequences contained two RNA-recognition and RNA-binding motifs in the N-terminus and an ascidian-specific YG-rich domain in the C-terminus. Maternal transcripts of musashi were ubiquitous in early cleavage-stage embryos. Ascidian musashi had three domains of zygotic expression: the brain, nerve cord, and mesenchyma. The temporal order of the onset in these domains was highly divergent between the two species of ascidian examined.
-
General Acid-Base Catalysis in the Mechanism of a Hepatitis Delta Virus Ribozyme. Reviewed
S. Nakano, D. M. Chadalavada, and P. C. Bevilacqua
Science 287 1493 - 1497 2000.2
-
Expression patterns of musashi homologus of the ascidians, Halocynthia roretzi and Ciona intestinalis.
西方敬人
Devl. Genes Evol ( 210 ) 162 - 165 2000
Single Work
-
Zoospore-specific antigen as a useful marker for molecular analysis of net formation in Hydrodictyon reticulatum (Chlorococcales, Chlorophyceae).
西方敬人
Phycol. Res ( 48 ) 143 - 148 2000
Single Work
-
Improved antigen detection on Western-blots.
西方敬人
Tech. Tips Online http://www.elsevier.com/locate/tto/ T02043 2000
Single Work
-
The transcript coding for an RNA-binding protein is localized in the anterior side of the ascidian 2-cell stage embryo.
西方敬人
Devl. Gene Evol. ( 210 ) 464 - 466 2000
Single Work
-
An ascidian glycine-rich RNA-binding protein is not induced by temperature-stress but is expressed under a genetic program during embryogenesis.
西方敬人
Gene ( 243 ) 207 - 214 2000
Single Work
-
Zoospore-specific antigen as a useful marker for molecular analysis of net formation in Hydrodictyon reticulatum(Chlorococcales, Chlorophyceae) Reviewed
Hatano, K., Yamamoto, M., Yamada, Y., Nishikata, T.
Phycol Res 48 143 - 148 2000
-
The transcript coding for an RNA-binding protein is localized in the anterior side of the ascidian 2-cell stage embryo Reviewed
Tanaka, K. J., Kawamura, H., Nishikata, T.
Devl. Gene Evol. 210 464 - 466 2000
Joint Work
-
Expression patterns of musashi homologus of the ascidians, Halocynthia roretzi and Ciona intestinalis Reviewed
Kawashima, T., Murakami, A. R., Ogasawara, M., Tanaka, K. J., Isoda, R., Sasakura, Y., Nishikata, T., Okano, H., Makabe, K. W.
Devl. Genes Evol. 210 162 - 165 2000
Joint Work
-
An ascidian glycine-rich RNA-binding protein is not induced by temperature-stress but is expressed under a genetic program during embryogenesis Reviewed
Tanaka, K. J., Kawamura, H., Matsugu, H., Nishikata, T.
Gene 243 207 - 214 2000
-
In situ spectroscopic and microscopic study on dispersion of Ag nanoparticles in polymer thin films
K. Akamatsu, N. Tsuboi, Y. Hatakenaka, S. Deki
J. Phys. Chem. B 104 10168 - 10173 2000
Joint Work
（学術雑誌、2000）104, 10168-10173
-
Au超微粒子を埋め込んだナイロン11蒸着膜の構造と物性
赤松謙祐、出来成人、井上翼、藤井稔、林真至
信学技報 OME 200-99 1 - 6 2000
Joint Work
（学術雑誌、2000）OME 200-99, 1-6
-
Preparation and characterization of polymer thin films containing silver and silver sulfide nanoparticles
K. Akamatsu, S. Takei, M. Mizuhata, A. Kajinami, S. Deki, S. Takeoka, M. Fujii, S. Hayashi, K. Yamamoto
Thin Solid Films 359 55 - 60 2000
Joint Work
（学術雑誌、2000）359, 55-60
-
Single-electron tunneling effects in thin nylon 11 films containing gold nanoparticles
Y. Inoue, M. Fujii, M. Inata, S. Hayashi, K. Yamamoto, K. Akamatsu, S. Deki
Thin Solid Films 372 169 - 172 2000
Joint Work
（学術雑誌、2000）372, 169-172
-
Formation of Co filled carbon nanocapsules by metal-template graphitization of diamond nanoparticles
S. Tomita, M. Hikita, M. Fujii, S. Hayashi, K. Akamatsu, S. Deki, H. Yasuda
J. Appl. Phys. 88 5452 - 5456 2000
Joint Work
（学術雑誌、2000）88, 5452-5456
-
Preparation and structure of copper nanoparticles/poly(acrylic acid) composite films
Y. Gotoh, R. Igarashi, Y. Ohkoshi, M. Nagura, K. Akamatsu, S. Deki
J. Mater. Chem. 10 2548 - 2552 2000
Joint Work
（学術雑誌、2000）10, 2548-2552
-
In vitro selection of aptamers that act with Zn2+ Reviewed
J. Kawakami, H. Imanaka, Y. Yokota and N. Sugimoto
J. Inorg. Biochem. 82 197 - 206 2000
-
Analysis for peptide binding to porphyrin using surface plasmon resonance
C. Nakamura, Y. Inuyama, K. Shirai, S. Nakano, N. Sugimoto, and J. Miyake
Synthetic Metals 117 127 - 129 2000
-
"A New Trend in Iron-Dithiocarbamate Complexes : As an Endogenous NO Trapping Agent. " Invited Reviewed
FUJII Satoshi
Coord. Chem. Rev. 198 89 - 99 2000
Single Work
-
Cycloprodigiosin hydrochloride, H(+)/CL(-) symporter, induces apoptosis and differentiation in HL-60 cells Reviewed
Yamamoto D, Uemura Y, Tanaka K, Nakai K, Yamamoto C, Takemoto H, Kamata K, Hirata H, Hioki K
International Journal of Cancer 88 2000
-
N-acetylcysteine suppresses TNF-induced NF-kappaB activation through inhibition of IkappaB kinases Reviewed
Oka S, Kamata H, Kamata K, Yagisawa H, Hirata H
FEBS Letters 2000
-
Demonstration of Cl- requirement for inhibition of vacuolar acidification by cycloprodigiosin in situ Reviewed
Nakayasu T, Kawauchi K, Hirata H, Shimmen T
Plant & Cell Physiol 41 2000
-
A Novel Screening Method for Combinatorial Chemistry for Low Affinity Interactions.
三好大輔
Chem. Commun. 677 - 678 1999.11
Single Work
-
Selection of peptides that recognize tryptophan by combinatorial chemistry
三好大輔
Peptide Science 281 - 284 1999.11
Single Work
-
a-b Structural Transition of Short Oligopeptides by Water/Organic Solvent Titration.
三好大輔
Chem. Lett 637 - 638 1999.11
Single Work
-
"四塩化炭素肝障害にて発生した一酸化窒素のジチオカルボキシサルコシン鉄錯体による捕捉"
藤井 敏司
磁気共鳴と医学(日本医学館) ( 10 ) 16 - 20 1999.11
Single Work
-
"In vivo Distribution and Behavior of Paramagnetic Dinitrosyl Dithiolato Iron Complex in the Abdomen of Mouse"
藤井 敏司
Free Radic. Res. ( 31 ) 525 - 534 1999.11
Single Work
-
"Direct Evidence of In Vivo Nitric Oxide Production and Inducible Nitric Oxide Synthase mRNA Expression in the Rat Brain of Living Rat During Experimental Meningitis"
藤井 敏司
J. Cereb. Blood Flow Metab ( 19 ) 1175 - 1178 1999.11
Single Work
-
"内因性NOドナー(NOチオレート鉄錯体)のマウス腹部における分布,動態に関する研究"
藤井 敏司
G.I.Research ( 7 ) 85 1999.11
Single Work
-
"内因性一酸化窒素のジチオカルバメート誘導体鉄錯体による捕捉検出～検出法の基礎的検討～"
藤井 敏司
磁気共鳴と医学(日本医学館) ( 10 ) 21 - 25 1999.11
Single Work
-
Nucleic Acid Duplex Stability : Influence of Base Composition on Cation Effects. Reviewed
S. Nakano, M. Fujimoto, H. Hara, and N. Sugimoto
Nucleic Acids Res. 27 2957 - 2965 1999.7
-
Generation and characterization of a monoclonal antibody, mH1, raised against mitotic HeLa cells.
西方敬人
Devl. Growth Differ ( 41 ) 381 - 389 1999
Single Work
-
A non-radioactive gel shift protocol enables recovery of RNA-binding proteins.
西方敬人
Tech. Tips Online http://www.elsevier.com/locate/tto/T01794 1999
Single Work
-
Chracterization of MTR1: A novel protein correlated with the myoplasmic movement during ascidian ooplasmic segregation.
西方敬人
Mem. Konan Univ., Sci. Ser. ( 46 ) 25 - 33 1999
Single Work
-
The CAB (Centrosome-Attracting Body), microtubule system and posterior egg cytoplasm are involved in positioning of cleavage planes in the ascidian embryo.
西方敬人
Devl. Biol ( 209 ) 72 - 85 1999
Single Work
-
The expression of the protochordate homologue of the proteasome regulatory subunit Rpn12 is transcriptionally , and post-translationally regulated during cleavage stage.
西方敬人
Zool. Sci. ( 16 ) 125 - 129 1999
Single Work
-
Interactions between cytoskeletal components during ooplasmic segregation in the myoplasm of ascidian eggs.
西方敬人
Devl. Growth Differ. ( 41 ) 265 - 272 1999
Single Work
-
The expression of the protochordate homologue of the proteasome regulatory subunit Rpn12 is transcriptionally and post-translationally regulated during cleavage stage.(共著) Reviewed
NISHIKATA Takahito
Zool. Sci. 16, 125-129 1999
-
Generation and characterization of a monoclonal antibody, mH1, raised against mitotic HeLa cells.(共著) Reviewed
NISHIKATA Takahito
Devl. Growth Differ. 41, 381-389 1999
-
Interactions between cytoskeletal components during ooplasmic segregation in the myoplasm of ascidian eggs.(共著) Reviewed
NISHIKATA Takahito
Devl. Growth Differ. 41, 265-272 1999
-
The CAB(Centrosome-Attracting Body), microtubule system, and posterior egg cytoplasm are involved in positioning of cleavage planes in the ascidian embryo.(共著) Reviewed
NISHIKATA Takahito
Devl. Biol. 209, 72-85 1999
-
A non-radioactive gel shift protocol enables recovery of RNA-binding proteins Reviewed
Tanaka, K. J., Nishikata, T.
Tech. Tips Online 4 ( 1 ) 70 - 72 1999
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Characterization of MTR1: A novel protein correlated with the myoplasmic movement during ascidian ooplasmic segregation.
Nakamori, R., Murakami, A., and Nishikata, T.
Memoirs of Konan University, Science and Engineering Series. 46 25 - 33 1999
Single Work
-
Characterization of MTR1 : A novel protein correlated with the myoplasmic movement during ascidian ooplasmic segregation.(共著)
NISHIKATA Takahito
Mem. Konan Univ. Sci. Ser 46, 25-33 1999
Single Work
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TEM investigation and electron diffraction study on dispersion of gold nanoparticles into nylon 11 thin film during heat treatment
K. Akamatsu, S. Deki
J. Colloid Interface Sci. 214 353 - 361 1999
Joint Work
（学術雑誌、1999）214, 353-361
-
Novel preparation method of nano-sized gold particles by using NH2-terminated polyehtylenoxide
S. Deki, K. Sayo, A. Yamada, K. Akamatsu, S. Hayashi
J. Colloid Interface Sci. 214 123 - 125 1999
Joint Work
（学術雑誌、1999）214, 123-125
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Synthesis and characterization of gold-palladium bimetallic particles dispersed in polymer thin film matrix
S. Deki, K. Akamatsu, Y. Hatakenaka, M. Mizuhata, A. Kajinami
Nanostruct. Mater. 11 59 - 65 1999
Joint Work
（学術雑誌、1999）11, 59-65
-
Preparation, characterization and optical properties of nano-sized bimetallic particles dispersed in polymer thin films
K. Akamatsu, Y. Hatakenaka, M. Mizuhata, A. Kajinami, S. Deki
Trans. Mater. Res. Soc. Jpn. 24 109 - 112 1999
Joint Work
（学術雑誌、1999）24, 109-112
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A selection of short peptides that interact with a porphyrin as a small target by immobilized phage display Reviewed
J. Kawakami, T. Kitano and N. Sugimoto
Chem. Commun. 1765 - 1766 1999
-
Development of New Biotechnology for the 21st Century ((]G0002[))-Structural and thermodynamic studies of PNA/DNA hybrids containing single bulge nucleotides-
Mem, Konan Univ., Sci. Ser. 46 61 1999
Single Work
-
"Direct Evidence of In Vivo Nitric Oxide Production and Inducible Nitric Oxide Synthase mRNA Expression in the Rat Brain of Living Rat During Experimental Meningitis" Reviewed
FUJII Satoshi
J. Cereb. Blood Flow Metab. 19 1175 - 1178 1999
Single Work
-
Cycloprodigiosin hydrochloride, a new H(+)/Cl(-) symporter, induces apoptosis in human and rat hepatocellular cancer cell lines in vitro and inhibits the growth of hepatocellular carcinoma xenografts in nude mice Reviewed
Yamamoto C, Takemoto H, Kuno K, Yamamoto D, Tsubura A, Kamata K, Hirata H, Yamamoto A, Kano H, Seki T, Inoue K
Hepatology 1999
-
Cycloprodigiosin hydrochloride inhibits acidification of plant vacuole Reviewed
Nakayasu T, Kawauchi K, Hirata H, Shimmen T
Plant & Cell Physiol 40 1999
-
Cycloprodigiosin hydrochloride obtained from Pseudoalteromonas denitrificans is a potent antimalarial agent Reviewed
Kim HS, Hayashi M, Shibata Y, Wataya Y, Mitamura T, Horii T, Kawauchi K, Hirata H, Tsuboi S, Moriyama Y
Biol. Pharm. Bull 22 1999
-
Cycloprodigiosin uncouples H+-pyrophosphatase of plant vacuolar membranes in the presence of chloride ionMaeshima Reviewed
M, Nakayasu, T, Kawauchi, K, Hirata, H, Shimmen, T
Plant & Cell Physiol 1999
-
Single-Stranded DNA Recognition of a 24 mer Peptide Derived from RecA Protein. Reviewed
S. Nakano and N. Sugimoto
Bull. Chem. Soc. Jpn. 71 2205 - 2210 1998.11
Single Work
-
Effect of Cation Concentration on Duplex Stability of Nucleic Acids with Unnatural Base Pairs.
中野 修一
Nucleic Acids Symp. Ser. 39 121 - 122 1998.11
Single Work
-
Synthesis and Applications of Molecularly Imprinted Artificial Receptors
Jun Matsui
1998.3
Single Work
-
Isolation and characterization of cDNA clones for epidermis-specific and musclespecific benes in Ciona savignyi embryos. Reviewed
NISHIKATA Takahito
Zoological Science 15 ( 2 ) 239 - 246 1998
-
An alternatively spliced gene encoding a Y-box protein showing maternal expression and tissue-specific zygotic expression in the ascidian embryos.(共著) Reviewed
NISHIKATA Takahito
Development, Growth and Differentiation 40 ( 6 ) 631 - 640 1998
-
Centrosome-attracting body : A novel structure closely related to unequal cleavages in the ascidian embryo. Reviewed
NISHIKATA Takahito
Development, Growth & Differentiation 40 ( 1 ) 85 - 95 1998
-
Dispersion of gold nanoparticles into a nylon 11 thin film during heat treatment : in situ optical transmission study
K. Akamatsu, S. Deki
J. Mater. Chem. 8 637 - 640 1998
Joint Work
-
Preparation and characterization of copper(I) oxide nanoparticles dispersed in polymer matrix
S. Deki, K. Akamatsu, T. Yano, M. Mizuhata, and A. Kajinami
J. Mater. Chem. 8 1865 - 1868 1998
Joint Work
-
Stability of DNA duplexes containing mirror-image DNA
J. Kawakami, K. Tsujita and N. Sugimoto
Nucleic Acids Symp. Ser. 39 55 - 56 1998
-
ニューバイオテクノロジー:ファージディスプレイ、インビトロセレクション及びコンビナトリアルケミストリー
川上 純司
化学 53 74 1998
Single Work
-
Minimization of genomic human hepatitis delta virus ribozyme Reviewed
H. Fauzi, A. Chiba, F. Nishikawa, M. Roy, J. Kawakami and S. Nishikawa
Anal. Chim. Acta 365 309 - 317 1998
-
In vitro selection of aptamers that recognize a monosaccharide
J. Kawakami, Y. Kawase and N. Sugimoto
Anal. Chim. Acta 365 95 - 100 1998
-
生命関連分子の微視的相互作用(3)-RNA鎖を切断するデオキシリボザイムと金属イオンの関係-
川上 純司
甲南大学紀要,理学編 45 125 1998
Single Work
-
生命関連分子の微視的相互作用(4)-ミスマッチを含むRNA/DNAハイブリッドの安定性に及ぼすミスマッチの隣接塩基対の影響-
川上 純司
甲南大学紀要,理学編 45 135 1998
Single Work
-
"Electron Paramagnetic Resonance Detection of Nitric Oxide in the Brain During Sepsis and Meningitis"
FUJII Satoshi
In"The Proceedings of the 2nd International Conference on Bioradicals" 146 - 148 1998
Single Work
-
A 24 mer Peptide Derived from RecA Protein can Discriminate a Single-Stranded DNA from a Double-Stranded DNA. Reviewed
N. Sugimoto and S. Nakano
Chem. Commun. 2125 - 2126 1997.11
-
A 24 mer Peptide with Activity of a Nucleic Acid Discrimination and Its Recombination.
中野 修一
First International Peptide Symp. ( P-108 ) 1997.11
Single Work
-
Experimental Quantitation of Cation Effect on Nucleic Acid Stability.
中野 修一
Nucleic Acids Symp. Ser. 37 147 - 148 1997.11
Single Work
-
Sandwiching Interaction of Peptides with a Porphyrin. Reviewed
N. Sugimoto and S. Nakano
Chem. Lett. 939 - 949 1997.11
Single Work
-
Cloning of the ascidian Y-box protein gene with a convenient and reliable amplification protocol of 3' ends of mRNAs.
NISHIKATA Takahito
Memoirs of Konan University, Science Series 44 ( 2 ) 45 - 51 1997
Single Work
-
Nanoscale metal particles dispersed in polymer matrix
K. Akamatsu, S. Deki
Nanostruct. Mater. 8 1121 - 1129 1997
Joint Work
-
Characterization and optical properties of gold nanoparticles dispersed in nylon 11 thin films
K. Akamatsu, S. Deki
J. Mater. Chem. 7 1773 - 1777 1997
Joint Work
-
Evolution of a phosphorothioate RNA library during in vitro selection
J. Kawakami and N. Sugimoto
Nucleic Acids Symp. Ser. 37 201 - 202 1997
-
生命関連分子の微視的相互作用(II)-ペプチドの分子内α-ヘリックスと分子間β-シートの構造遷移-
川上 純司
甲南大学紀要,理学編 44 17 1997
Single Work
-
Analysis of the cleavage reaction of a trans-acting human hepatitis delta virus ribozyme Reviewed
H. Fauzi, J. Kawakami, F. Nishikawa and S. Nishikawa
Nucleic Acids Research 25 3124 - 3130 1997
-
A possible immunosuppressant, cycloprodigiosin hydrochloride, obtained from Pseudoalteromonas denitrificans Reviewed
Kawauchi K, Shibutani K, Yagisawa H, Kamata H, Nakatsuji S, Anzai H, Yokoyama Y, Ikegami Y, Moriyama Y, Hirata H
Biochem. Biophys. Res. Commun, 237 1997
Authorship：Lead author
-
Development of New SPR Chip with an Oligopeptide Recognizing a Single-Stranded DNA.
中野 修一
Nucleic Acids Symp. Ser. 35 303 - 304 1996.11
Single Work
-
Improved Thermodynamic Parameters and Helix Initiation Factor to Predict Stability of DNA Duplexes. Reviewed
N. Sugimoto, S. Nakano, M. Yoneyama, and K. Honda
Nucleic Acids Res. 24 4501 - 4505 1996.11
-
Interactions of 5-(1-pyrenyl)-10,15,20,-tris(4-methyl-pyridinium)porphine with Double-Helix and Triple-Helix Nucleic Acids. Reviewed
N. Sugimoto, S. Nakano, M. Katoh, M. Sasaki, and S. Kugimiya
Nucleosides & Nucleotides 15 743 - 747 1996.8
-
Two distinct cell types identified in the ascidian notochord. Reviewed
NISHIKATA Takahito
Zoological Science 13 725 - 730 1996
Single Work
-
Molecalar characterization of myoplasmin-Cl : a cytoskeletal component localized in the myoplasm of the ascidian egg Reviewed
NISHIKATA Takahito, WADA Michiko
Development, Genes and Evolution 206 72 - 79 1996
-
Preparation and characterization of Au-dispersed TiO2 thin films by a liquid-phase deposition method
S. Deki, Y. Aoi, H. Yanagimoto, H. Ishii, K. Akamatsu, M. Mizuhata, A. Kajinami
J. Mater. Chem. 6 1879 - 1882 1996
Joint Work
-
Selection in vitro of trans-acting genomic human hepatitis delta virus (HDV) ribozymes Reviewed
F. Nishikawa, J. Kawakami, A. Chiba, K. Yuda, M. Shirai, P.K.R. Kumar and S. Nishikawa
Eur. J. Biochem. 327 712 - 718 1996
-
生命関連分子の微視的相互作用(I)-バルジを含むDNA二重らせんの熱力学的安定性に及ぼすイノシン置換の影響-
川上 純司
甲南大学紀要,理学編 43 37 - 47 1996
Single Work
-
Constructing an efficient trans-acting genomic HDV ribozyme Reviewed
J. Kawakami, K. Yuda, Y.-A. Suh, P.K.R. Kumar, F. Nishikawa, H. Maeda, K. Taira, E. Ohtsuka and S. Nishikawa
FEBS Lett. 394 132 - 136 1996
-
Thermodynamic Parameters to Predict Stability of RNA/DNA Hybrid Duplexes.
中野 修一
J. Biomol. Struct. Dyn. 12 ( a233 ) 1995.11
Single Work
-
Interaction of Oligonuleotides with a Single Stranded DNA Binding Site of RecA Protein.
中野 修一
Nucleic Acids Symp. Ser. 34 61 - 62 1995.11
Single Work
-
Thermodynamic stability of RNA/DNA hybrids. Reviewed
N. Sugimoto, S. Nakano, M. Katoh, A. Matsumura, H. Nakamuta, T. Ohmichi, M. Yoneyama, and M. Sasaki
Biochemistry 34 11211 - 11216 1995.9
-
ANALYSIS OF FUNCTIONAL STRUCTURE OF HDV RIBOZYME BY MODIFICATION INTERFERENCE AND IN-VITRO SELECTION-STRATEGIES
S NISHIKAWA, YH JEOUNG, P KUMAR, J KAWAKAMI, F NISHIKAWA, A CHIBA, K YUDA, P VILLJANEN, K TAIRA
JOURNAL OF CELLULAR BIOCHEMISTRY 19A 227 - 227 1995.1
Joint Work
Publisher：WILEY-LISS
-
Designing of HDV ribozyme by in vitro selection Reviewed
S. Nishikawa, F. Nishikawa, J. Kawakami, A. Chiba, K. Yuda, Y.-H. Jeoung, P.K.R. Kumar and K. Taira
Antisense Research and Development 54 104 - 105 1995
-
Thermodynamic Parameters to Predict the Stability of RNA/DNA Hybrids in Antisense Interaction.
中野 修一
First International Antisence Conference of Japan ( P1-05 ) 1994.11
Single Work
-
RNA/DNA Hybrid Duplexes with Identical Nearest-Neighbor Base-Pairs Have Identical Stability. Reviewed
N. Sugimoto, M. Katoh, S. Nakano, T. Ohmichi, and M. Sasaki
FEBS Lett. 354 74 - 78 1994.10
-
ヒトδ型肝炎ウイルス（HDV）リボザイムの構造活性相関に関する研究
川上純司
1994.3
Single Work
-
Specification in the primary lineage of ascidian muscle cells analyzed with a specific monoclonal antibody
NISHIKATA Takahito
Memoirs of Konan University Science Series 41 ( 2 ) 89 - 97 1994
Single Work
-
Monoclonal antibodies with affinity to self-complementary left-handed DNA containing cyclonucleosides with high anti conformation Reviewed
J. Kawakami, S. Uesugi, M. Ikehara, T. Itoh, K. Miura and E. Ohtsuka
Nucleosides & Nucleotides 13 421 - 427 1994
-
The accumulation of vanadium and manifestation of an antigen recognized by a monoclonal antibody specific to vanadocytes during embryo genesis in vanadium-rich ascidian, ┣DBAsidia(/)-┫DB ┣DBSydneieusis(/)-┫DB ┣DBsamea(/)-┫DB. Reviewed
NISHIKATA Takahito
Journal of Experimental Zoology 265,29-34 1993
Single Work
-
Systematic substitution of individual bases in two important single-stranded regions of the HDV ribozyme for evaluation of the role of specific bases Reviewed
Y.-A. Suh, P.K.R. Kumar, J. Kawakami, F. Nishikawa, K. Taira, and S. Nishikawa
FEBS Lett. 326 158 - 162 1993
-
Chemical probing studies of the hepatitis delta virus (HDV) genomic ribozyme
S. Nishikawa, P.K.R. Kumar, Y.-H. Jeong, J. Kawakami, F. Nishikawa, Y.-A. Suh, E. Ohtsuka and K. Taira
Nucleic Acids Symp. Ser. 29 119 - 120 1993
-
Identification of important bases in a single-stranded region (SSrC) of the hepatitis delta ribozyme Reviewed
J. Kawakami, P.K.R. Kumar, Y.-A. Suh, F. Nishikawa, K. Kawakami, K. Taira, E. Ohtsuka and S. Nishikawa
Eur. J. Biochem. 217 29 - 36 1993
-
Initial stages of tunic morphogenesis in the ascidian ┣DBHalocythia(/)-┫DB : a fine structural study. Reviewed
NISHIKATA Takahito
Tissue and Cell 24 1992
Single Work
-
Random mutations to evaluate the role of bases at two important single-stranded regions of genomic HDV ribozyme Reviewed
P.K.R. Kumar, Y.-A. Suh, H. Miyashiro, F. Nishikawa, J. Kawakami, K. Taira and S. Nishikawa
Nucleic Acids Research 20 3919 - 3924 1992
-
Ribozymes as molecular scissors for suppressing AIDS virus Reviewed
J. Ohkawa, T. Shimayama, N. Yuyama, S. Sawata, Y. Takagi, A. Wada, K. Yasuda, J. Kawakami, P.K.R. Kumar, Y.-A. Suh, F. Nishikawa, M. Oda, S. Shinshi, M. Uebayashi, T. Uchimaru, K. Furukawa, S. Nishikawa and K. Taira
Now & Future 8 8 - 12 1992
-
Identification of important bases for the self-cleavage activity at two single-stranded regions of genomic HDV ribozyme
Nucleic Acids Symposium Series 27 41 1992
Single Work
-
Monoclonal antibody specific to signet ring cells, the vanadocytes of the tunicate, ┣DBAscidia(/)-┫DB ┣DBsydneiensis(/)-┫DB ┣DBsamea(/)-┫DB. Reviewed
NISHIKATA Takahito
Journal of Experimental Zoology 259 1991
Single Work
-
Characterization of antigenic polypeptides recognized by monoclonal antibodies specific to the myoplasm of eggs of the ascidian ┣DBCiona(/)-┫DB ┣DBinterstinalis(/)-┫DB. Reviewed
NISHIKATA Takahito
Development, Growth and Differentiation 33 1991
-
Expression of an antigen specific for trunk-lateral cells in quarter embryos of the ascidian, ┣DBHalocynthia(/)-┫DB ┣DBroretzi(/)-┫DB. Reviewed
NISHIKATA Takahito
Journal of Experimental Zoology 258 1991
Single Work
-
Specification of notochord cells in the ascidian embryo analysed with a specific monoclonal antibody. Reviewed
NISHIKATA Takahito
Cell Differentiation and Development 30 1990
Single Work
-
Cellular and molecular mechanisms of muscle cell differentiation in the ascidian embryos. Reviewed
NISHIKATA Takahito
International Review of Cytology 122 1990
Single Work
-
Egg cytoplasmic components responsible for larval muscle cell differentiation in ascidian embryos.
NISHIKATA Takahito
Developmental Biology UCLA Symposia 125 1990
Single Work
-
ホヤ卵における筋肉細胞分化決定因子の分子的実体の解析
西方敬人
1989.3
Single Work
-
A monoclonal antibody specific to embryoie trunk-lateral cells of the ascidian ┣DBHalocynthia(/)-┫DB ┣DBroretzi(/)-┫DB stains coelomic cells of juvenile and adult basophilic blood cells. Reviewed
NISHIKATA Takahito
Development, Growth and Differentiation 31 ( 6 ) 595 - 600 1989
-
Differentiation expression in blastomeres of cleavagearrested embryos of the ascidian ┣DBHalocynthia(/)-┫DB ┣DBroretzi(/)-┫DB. Reviewed
NISHIKATA Takahito
Development, Growth and Differentiation 30 ( 4 ) 1988
-
Muscle cell differentiation in ascidian embryos analysed with a tissue-specific monoclonal antibody. Reviewed
NISHIKATA Takahito
Development 99 ( 2 ) 1987
Single Work
-
Monoclonal antibodies against components of the myoplasm of eggs of the ascidian ┣DBCiona(/)-┫DB ┣DBintestinalis(/)-┫DB partially block the development of muscle-specific acetylcholinesterase. Reviewed
NISHIKATA Takahito
Development 100 ( 2 ) 1987
Single Work
-
Cell-and tissue-specific monoclonal antibodies in eggs and embryos of the ascidian ┣DBHalocynthia(/)-┫DB ┣DBroretzi(/)-┫DB. Reviewed
NISHIKATA Takahito
Development 99 ( 2 ) 1987
Single Work
-
Expression of epidermis-specific antigens during embryogenesis of the ascidian, ┣DBHalocynthia(/)-┫DB ┣DBroretzi(/)-┫DB. Reviewed
NISHIKATA Takahito
Developmental Biology 121 ( 2 ) 408 - 416 1987