Faculty of Frontiers of Innovative Research in Science and Technology

Papers -

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Comprehensive analysis of stability and variability of DNA minimal i-motif structures Invited Reviewed

K. Ashida, A. Kitabayashi, K. Nishiyama, and S. Nakano

Molecules 30 1831 2025.4

DOI： 10.3390/molecules30081831

One-Pot Synthesis and Immobilization of Gold Nanoparticles Using Peptidyl Microbeads

Shuhei Yoshida, Koki Yoshida, Taichi Isozaki, Maho Oura, Makoto Ozaki, Takaaki Tsuruoka, Kenji Usui

Molecules 30 1689 2025.4

DOI： 10.3390/molecules30081689

Liquid-liquid phase separation induced by i-motif DNA under molecular crowding conditions Invited Reviewed

Ryosuke Suzuki, Mitsuki Tsuruta, Sumit Shil, Kosei Morohashi, Keiko Kawauchi, Daisuke Miyoshia

Polymer J. 2025.4

Authorship：Corresponding author

Tendon Tissue Regeneration With Cell Orientation Using an Injectable Alginate-Cell Cross-linked Gel Reviewed

Jun Yamaguchi, Kentaro Homan, Tomohiro Onodera, Masatake Matsuoka, Shoutaro Arakawa, Natsumi Ueda, Nana Kawate, Takayuki Nonoyama, Yoshinori Katsuyama, Koji Nagahama, Mitsuru Saito, Norimasa Iwasaki

The American Journal of Sports Medicine 2025.3

Three- and four-stranded nucleic acid structures and their ligands Invited Reviewed

Yoshiki Hashimoto, Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, and Daisuke Miyoshi

RSC Chemical Biology 6 466 - 491 2025.3

Authorship：Corresponding author

DOI： 10.1039/D4CB00287C

ケミカルバイオロジーの冒険（１２）　核酸を標的とした低分子・中分子化合物 Invited

堂野主税、勝田陽介、三好大輔

現代化学 648 32 - 39 2025.2

Authorship：Last author

Roles of Loop Region in Folding Kinetics and Transcription Inhibition of DNA G-Quadruplexes Reviewed

64 609 - 619 2025.2

Development of a New Structural Transformation Reaction in Cr-Based MOFs Induced by Fluoride Reviewed

Shintaro Tanaka, Ryoya Miyata, Reon Teramoto, Junya Sugimoto, Takaaki Tsuruoka, Kensuke Akamatsu, Yohei Takashima

Chem. Asian J e202401717 2025.2

Authorship：Last author,　Corresponding author

Development of detection system for lead ions in mixture solutions using UV-Vis measurements with peptide immobilized microbeads

Shuhei Yoshida, Koki Yoshida, Yoshio Hamada, Takaaki Tsuruoka, Kenji Usui

Scientific Reports 15 3249 2025.1

Complex and Non-sequential Current Signatures of a β-Hairpin Peptide Confined in a Nanopore International coauthorship

Misa Yamaji, Mauro Chinappi, Blasco Morozzo della Rocca, Kenji Usui, Ryuji Kawano

Analytical Chemistry 97 2044 - 2051 2025.1

Publisher：American Chemical Society (ACS)

DOI： 10.1021/acs.analchem.4c04150

Roles of Loop Region in Folding Kinetics and Transcription Inhibition of DNA G-Quadruplexes Reviewed

Minori Nakata, Naoki Kosaka, Keiko Kawauchi, Daisuke Miyoshi

Biochemistry 64 609 - 619 2025.1

Authorship：Corresponding author

DOI： 10.1021/acs.biochem.4c00601

Factors affecting liquid-liquid phase separation of RGG peptides with DNA G-Quadruplex Reviewed

Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, Daisuke Miyoshi

ChemMedChem 20 ( 2 ) e202400460 2025.1

The role of cytosine methylation in regulating the topology and liquid-liquid phase separation of DNA G-quadruplexes Reviewed

Mitsuki Tsuruta, Sumit Shil, Shinya Taniguchi, Keiko Kawauchi, Daisuke Miyoshi

Chemical Science 16 ( 10 ) 4213 - 4225 2025.1

Sequence-Specific Free Energy Changes in DNA/RNA Induced by a Single LNA-T Modification in Antisense Oligonucleotides Reviewed

Elisa Tomita-Sudo, Tomoka Akita, Nae Sakimoto, Saori Tahara-Takamine, Junji Kawakami

International Journal of Molecular Sciences 25 13240 - 13240 2024.12

DOI： 10.3390/ ijms252413240 Academic

Bioinformatic analysis of actin-binding proteins in the nucleolus during heat shock Invited Reviewed

Shinya Taniguchi, Takeru Torii, Toshiyuki Goto, Kohei Takeuchi, Rine Katsumi, Mako Sumida, Sunmin Lee, Wataru Sugimoto, Masaya Gessho, Katsuhiko Itoh, Hiroaki Hirata, Junji Kawakami, Daisuke Miyoshi, Keiko Kawauchi

Genes 15 1580 2024.12

Authorship：Last author,　Corresponding author

L-Histidine Modulates the Catalytic Activity and Conformational Changes of the HD3 Deoxyribozyme Reviewed

Nae Sakimoto, Hirofumi Imanaka, Elisa Tomita-Sudo, Tomoka Akita, Junji Kawakami

genes 15 1481 2024.11

DOI： 10.3390/genes15111481

NRAS DNA G-quadruplex-targeting molecules for sequence-selective enzyme inhibition Reviewed

Yoshiki Hashimoto, Hiroki Kubo, Keiko Kawauchi, Daisuke Miyoshi

Chem. Commun. 60 13179 - 13182 2024.11

Detection of Conformational Changes of G4 Aptamer in the Presence of Different Cations using Calorimetry Invited Reviewed

Elisa Tomita-Sudo, Taku Ishigaki, Masako Hirose, Maki Kato, and Junji Kawakami

Netsu Sokutei 51 ( 4 ) 148 - 152 2024.10

Authorship：Last author

NRAS DNA G-quadruplex-targeting molecules for sequence-selective enzyme inhibition Invited Reviewed

Yoshiki Hashimoto, Hiroki Kubo, Keiko Kawauchi and Daisuke Miyoshi

Chem. Commun. 2024.10

Authorship：Corresponding author

DOI： 10.1039/D4CC03753G

The role of cytosine methylation in regulating the topology and liquid-liquid phase separation of DNA G-quadruplexes Reviewed

Mitsuki Tsuruta, Sumit Shil, Shinya Taniguchi, Keiko Kawauchi and Daisuke Miyoshi

Chem. Sci. 16 4213 - 4225 2024.10

Authorship：Corresponding author

DOI： 10.1039/D4SC06959E

Structural transformation of silver(I)-thiolate coordination plymer film at solid-liquid interfaces Reviewed International coauthorship

Myu Fukuoka, Yohei Takashima, Kensuke Akamatsu, Aude Demessence, Takaaki Tsuruoka

CrystEngComm 26 6269 - 6273 2024.10

Factors Affecting Liquid-Liquid Phase Separation of RGG Peptides with DNA G-Quadruplex Invited Reviewed

Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, Daisuke Miyoshi

ChemMedChem 2024.9

DOI： 10.1002/cmdc.202400460(2024) [in press]

Bioinformatic Analysis of Actin-Binding Proteins in the Nucleolus During Heat Shock Invited Reviewed

Shinya Taniguchi, Takeru Torii ,Toshiyuki Goto, Kohei Takeuchi, Rine Katsumi, Mako Sumida, Sunmin Lee, Wataru Sugimoto, Masaya Gessho, Katsuhiko Itoh, Hiroaki Hirata, Junji Kawakami, Daisuke Miyoshi and Keiko Kawauchi

Genes 15 ( 12 ) 1580 2024.9

DOI： 10.3390/genes15121580

Non-canonical olfactory pathway activation induces cell fusion of cervical cancer cells. Reviewed International journal

Keigo Araki, Takeru Torii, Kohei Takeuchi, Natsuki Kinoshita, Ryoto Urano, Rinka Nakajima, Yaxuan Zhou, Tokuo Kobayashi, Tadayoshi Hanyu, Kiyoshi Ohtani, Kimiharu Ambe, Keiko Kawauchi

Neoplasia 57 101044 - 101044 2024.8

Authorship：Last author

Multinucleation occurs in various types of advanced cancers and contributes to their malignant characteristics, including anticancer drug resistance. Therefore, inhibiting multinucleation can improve cancer prognosis; however, the molecular mechanisms underlying multinucleation remain elusive. Here, we introduced a genetic mutation in cervical cancer cells to induce cell fusion-mediated multinucleation. The olfactory receptor OR1N2 was heterozygously mutated in these fused cells; the same OR1N2 mutation was detected in multinucleated cells from clinical cervical cancer specimens. The mutation-induced structural change in the OR1N2 protein activated protein kinase A (PKA), which, in turn, mediated the non-canonical olfactory pathway. PKA phosphorylated and activated furin protease, resulting in the cleavage of the fusogenic protein syncytin-1. Because this cleaved form of syncytin-1, processed by furin, participates in cell fusion, furin inhibitors could suppress multinucleation and reduce surviving cell numbers after anticancer drug treatment. The improved anticancer drug efficacy indicates a promising therapeutic approach for advanced cervical cancers.

DOI： 10.1016/j.neo.2024.101044

PubMed

Quantitative Effects of the Loop Region on Topology, Thermodynamics, and Cation Binding of DNA G-quadruplexes Reviewed

ACS Omega 9 35028 - 35036 2024.7

Authorship：Last author,　Corresponding author

DOI： 10.1021/acsomega.4c05008

Development of a CaCO3 Precipitation Method Using a Peptide and Microwaves Generated by a Magnetron Reviewed

Fumihiro Kayamori, Hiroyuki Togashi, Natsumi Endo, Makoto Ozaki, Kan Hirao, Yonejiro Arimoto, Ryuji Osawa, Takaaki Tsuruoka, Takahito Imai, Kin-ya Tomizaki, Tomohiro Umetani, Nobuhiro Nakanishi, Kenji Usui

Processes 12 1327 - 1327 2024.6

Publisher：MDPI AG

Microwave applications, such as microwave ovens and mobile phones, are ubiquitous and indispensable in modern society. As the utilization of microwave technology is becoming more widespread, the effects of microwaves on living organisms and physiological processes have received increased attention. This study aimed to investigate the effects of microwaves on calcium carbonate biomineralization as a model biochemical process. A magnetron oscillator was used to generate 2450 MHz microwaves because magnetrons are relatively inexpensive and widespread. We conducted transmission electron microscopy (TEM), atomic force microscopy (AFM), TEM-electron energy-loss spectroscopy (EELS), dynamic light scattering (DLS), and high-performance liquid chromatography (HPLC) measurements to analyze the calcium carbonate precipitates. Our findings showed the formation of string-like precipitates of calcium carbonate upon microwave irradiation from one direction, similar to those obtained using a semiconductor oscillator, as reported previously. This implied that the distribution of the frequency had little effect on the morphology. Furthermore, spherical precipitates were obtained upon microwave irradiation from two directions, indicating that the morphology could be controlled by varying the direction of microwave irradiation. Magnetrons are versatile and also used in large-scale production; thus, this method has potential in medical and industrial applications.

DOI： 10.3390/pr12071327

Development of a CaCO3 Precipitation Method Using a Peptide and Microwaves Generated by a Magnetron Reviewed

Fumihiro Kayamori Hiroyuki Togashi Natsumi Endo Makoto Ozaki Kan Hirao Yonejiro Arimoto Ryuji Osawa Takaaki Tsuruoka Takahito Imai Kin-ya Tomizaki Tomohiro Umetani Nobuhiro Nakanishi Kenji Usui

Processes 12 ( 7 ) 1327 2024.6

Inhibition of RNA phosphodiester backbone cleavage in the presence of organic cations of different sizes Invited Reviewed International journal

S. Yoshioka, A. Doi, and S. Nakano

ChemBioChem 25 ( 13 ) e202400276 2024.5

Joint Work

Authorship：Last author,　Corresponding author Publisher：Wiley

Living cells contain various types of organic cations that may interact with nucleic acids. In order to understand the nucleic acid–binding properties of organic cations of different sizes, we investigated the ability of simple organic cations to inhibit the RNA phosphodiester bond cleavage promoted by Mg2+, Pb2+, and RNA-cleaving serum proteins. Kinetic analysis using chimeric DNA–RNA oligonucleotides showed that the cleavage at ribonucleotide sites was inhibited in the presence of monovalent cations comprising alkyl chains or benzene rings. The comparison of the cleavage rates in the presence of quaternary ammonium and phosphonium ions indicated that the steric hindrance effect of organic cations on their binding to the RNA backbone is significant when the cation size is larger than the phosphate–phosphate distance of a single-stranded nucleic acid. The cleavage inhibition was also observed for ribonucleotides located in long loops but not in short loops of oligonucleotide structures, indicating less efficient binding of bulky cations to structurally constrained regions. These results reveal the unique nucleic acid–binding properties of bulky cations distinct from those of metal ions.

DOI： 10.1002/cbic.202400276.

Interfacial analysis of the ion-transport process controlling the steady-state current in a two-phase electrodeposition system using polyelectrolyte membranes Reviewed

Shunsuke Yamada, Yohei Takashima, Takaaki Tsuruoka, Kensuke Akamatsu

RSC Appl. Interfaces 1 1069 - 1076 2024.5

Separation of the diastereomers of phosphorothioated siRNAs by anion-exchange chromatography under non-denaturing conditions Reviewed International journal

Hiroyuki Togawa, Takashi Okubo, Kazuki Horiuchi, Takao Yamaguchi, Elisa Tomita-Sudo, Tomoka Akita, Junji Kawakami, Satoshi Obika

Journal of Chromatography A 1721 464847 2024.4

Joint Work

In recent years, several small interfering RNA (siRNA) therapeutics have been approved, and most of them are phosphorothioate (PS)-modified for improving nuclease resistance. This chemical modification induces chirality in the phosphorus atom, leading to the formation of diastereomers. Recent studies have revealed that Sp and Rp configurations of PS modifications of siRNAs have different biological properties, such as nuclease resistance and RNA-induced silencing complex (RISC) loading. These results highlight the importance of determining diastereomeric distribution in quality control. Although various analytical approaches have been used to separate diastereomers (mainly single-stranded oligonucleotides), it becomes more difficult to separate all of them as the number of PS modifications increases. Despite siRNA exhibits efficacy in the double-stranded form, few reports have examined the separation of diastereomers in the double-stranded form. In this study, we investigated the applicability of non-denaturing anion-exchange chromatography (AEX) for the separation of PS-modified siRNA diastereomers. Separation of the four isomers of the two PS bonds tended to improve in the double-stranded form compared to the single-stranded form. In addition, the effects of the analytical conditions and PS-modified position on the separation were evaluated. Moreover, the elution order of the Sp and Rp configurations was confirmed, and the steric difference between them, i.e., the direction of the anionic sulfur atom, appeared to be important for the separation mechanism in non-denaturing AEX. Consequently, all 16 peak tops of the four PS modifications were detected in one sequence, and approximately 30 peak tops were detected out of 64 isomers of six PS bonds, indicating that non-denaturing AEX is a useful technique for the quality control of PS-modified siRNA therapeutics.

DOI： 10.1016/j.chroma.2024.464847

細胞内環境で安定化する核酸構造を標的にした低分子薬の開発 Invited

橋本佳樹, 川内敬子, 三好大輔

MEDCHEM NEWS (日本薬学会） 34 ( 1 ) 36 - 42 2024.4

Authorship：Last author,　Corresponding author

DOI： 10.14894/medchem.34.1_36

Basic protein- and peptide-induced stabilization of long-loop DNA G-quadruplexes Reviewed International journal

K. Tanabe, K. Miyazaki, H. Umeno, M. Takemoto, and S. Nakano

Biochimie 219 110 - 117 2024.4

Joint Work

Authorship：Last author,　Corresponding author Publisher：Elsevier

The human genome contains many G-quadruplex-forming sequences, including sequences containing long single-stranded loops that are believed to be unfavorable for G-quadruplex formation. The intracellular environment of biological cells is crowded with proteins with charged surfaces. Understanding the effects of protein-rich environments is important for understanding the formation of G-quadruplexes in an intracellular environment. In this study, we investigated the structural stability of DNA G-quadruplexes in the presence of several types of globular proteins (lysozyme, cytochrome c, bovine serum albumin, myoglobin, histone proteins, and serum proteins), unstructured polypeptides (protamine and poly-l-lysine), and oligopeptides (RGG/RG-domain peptides and short repeated peptides). Thermal melting studies of G-quadruplex-forming oligonucleotides derived from the human telomeric repeat sequence revealed that environments containing high concentrations of proteins and peptides differently affected the G-quadruplex stability according to their loop lengths. We found that weak electrostatic interactions of G-quadruplex loops with basic proteins and peptides improved the stability of long-loop G-quadruplexes and the interactions were strengthened under crowded conditions simulated by dextran. The comparison of the effects of different types of proteins and peptides indicated that excluded volume interactions and structural flexibility of both DNA and polypeptide chains influenced the efficiency of their interactions. This study provides insights into long-loop G-quadruplex stability in a crowded intracellular environment and the recognition of G-quadruplexes by arginine-rich domains of G-quadruplex-binding proteins.

DOI： 10.1016/j.biochi.2023.11.005

Development of a Pseudocellular System to Quantify Specific Interactions Determining the G-Quadruplex Function in Cells Reviewed

Hisae Tateishi-Karimata, Keiko Kawauchi, Shuntaro Takahashi, Naoki Sugimoto

Journal of the American Chemical Society 146 ( 12 ) 8005 - 8015 2024.3

Publisher：American Chemical Society (ACS)

DOI： 10.1021/jacs.3c11160

Cr(NO3)3 as a New Etching Reagent for an Al-Based Metal–Organic Framework to Control Its Crystal Size and Defects Reviewed

Yohei Takashima, Naoto Tanabe, Shintaro Tanaka, Takaaki Tsuruoka, Kensuke Akamatsu

Crystal Growth & Design 24 ( 4 ) 1766 - 1773 2024.2

Publisher：American Chemical Society (ACS)

DOI： 10.1021/acs.cgd.3c01405

Cr(NO3)3 as a new etching reagent for an Al-based metal-organic framework to control its crystal size and defects Reviewed

Yohei Takashima, Naoto Tanabe, Shintaro Tanaka, Takaaki Tsuruoka, Kensuke Akamatsu

Cryst. Growth Des. 24 1766 - 1773 2024.2

Authorship：Lead author,　Corresponding author

Structure and thermoelectric characterization of p-Type SnTe nanobulk material synthesized by charge compensation chemical reaction Reviewed

Ryosuke Fujiwara, Yuta Ikeda, Takuto Kawaguchi, Yohei Takashima, Takaaki Tsuruoka, Kensuke Akamatsu

Energies 17 190 2024.2

Inhibitory Effects of Shikonin Dispersion, an Extract of Lithospermum erythrorhizon Encapsulated in β-1,3-1,6 Glucan, on Streptococcus mutans and Non-Mutans Streptococci Reviewed

Ryota Nomura, Yuto Suehiro, Fumikazu Tojo, Saaya Matayoshi, Rena Okawa, Masakazu Hamada, Shuhei Naka, Michiyo Matsumoto-Nakano, Rika Unesaki, Kazuya Koumoto, Keiko Kawauchi, Takahito Nishikata, Tatsuya Akitomo, Chieko Mitsuhata, Masatoshi Yagi, Toshiro Mizoguchi, Koki Fujikawa, Taizo Taniguchi and Kazuhiko Nakano

25 1075 2024.1

Inhibitory Effects of Shikonin Dispersion, an Extract of Lithospermum erythrorhizon Encapsulated in β -1,3-1,6 Glucan, on Streptococcus mutans and Non-Mutans Streptococci Reviewed

Nomura R, Suehiro Y, Tojo F, Matayoshi S, Okawa R, Hamada M, Naka S, Matsumoto-Nakano M, Unesaki R, Koumoto K, Kawauchi K, Nishikata T, Akitomo T, Mitsuhata C, Yagi M, Mizoguchi T, Fujikawa K, Taniguchi T, Nakano K

International Journal of Molecular Science 25 1075 2024.1

Joint Work

DOI： doi.org/10.3390/ijms25021075

Inhibitory Effects of Shikonin Dispersion, an Extract of Lithospermum erythrorhizon Encapsulated in β-1,3-1,6 Glucan, on Streptococcus mutans and Non-Mutans Streptococci. Reviewed International journal

International journal of molecular sciences 25 ( 2 ) 2024.1

Shikonin is extracted from the roots of Lithospermum erythrorhizon, and shikonin extracts have been shown to have inhibitory effects on several bacteria. However, shikonin extracts are difficult to formulate because of their poor water solubility. In the present study, we prepared a shikonin dispersion, which was solubilized by the inclusion of β-1,3-1,6 glucan, and analysed the inhibitory effects of this dispersion on Streptococcus mutans and non-mutans streptococci. The shikonin dispersion showed pronounced anti-S. mutans activity, and inhibited growth of and biofilm formation by this bacterium. The shikonin dispersion also showed antimicrobial and antiproliferative effects against non-mutans streptococci. In addition, a clinical trial was conducted in which 20 subjects were asked to brush their teeth for 1 week using either shikonin dispersion-containing or non-containing toothpaste, respectively. The shikonin-containing toothpaste decreased the number of S. mutans in the oral cavity, while no such effect was observed after the use of the shikonin-free toothpaste. These results suggest that shikonin dispersion has an inhibitory effect on S. mutans and non-mutans streptococci, and toothpaste containing shikonin dispersion may be effective in preventing dental caries.

DOI： 10.3390/ijms25021075

PubMed

Direct Formation of ZIF-8 Crystal Thin Films on the Surface of a Zinc Ion-Doped Polymer Substrate Reviewed

Takaaki Tsuruoka, Kaito Araki, Kouga Kawauchi, Yohei Takashima, Kensuke Akamatsu

Inorganics 12 ( 1 ) 21 - 21 2024.1

Authorship：Lead author,　Corresponding author Publisher：MDPI AG

Thin films of metal–organic frameworks (MOFs) on polymer substrates and MOF/polymer mixed-matrix membranes play crucial roles in advancing the field of gas separation membranes. In this paper, we present a novel method for the direct formation of continuous ZIF-8 crystal films on a polymer substrate doped with Zn²+. Our approach involves ion exchange between the doped zinc ions within the substrate and sodium ions in the presence of a CH3COONa additive, as well as interfacial complexation with eluted zinc ions and 2-methylimidazole (2-MeIM). The key factors affecting the formation of ZIF-8 crystals on the substrate were the concentrations of CH3COONa and 2-MeIM. A time-course analysis revealed that the nucleation rate during the early stages of the reaction significantly affected the surface morphology of the resulting ZIF-8 crystal films. Specifically, a higher nucleation rate led to the formation of continuous small ZIF-8 crystal films. This innovative approach enables the fabrication of densely packed, uniform ZIF-8 crystal films.

DOI： 10.3390/inorganics12010021

Direct formation of ZIF-8 crystal thin films on the surface of a zinc ion-doped polymer substrate Reviewed

Takaaki Tsuruoka, Kaito Araki, Kouga Kawauchi, Yohei Takashima, Kensuke Akamatsu

Inorganics 12 21 2024.1

“Interfacial analysis of the ion-transport process controlling the steady-state current in a two-phase electrodeposition system using polyelectrolyte membranes

S. Yamada, Y. Takashima, T. Tsuruoka, K. Akamatsu

RSC Applied Interfaces 2024

Structural transformation of silver(I)-thiolate coordination polymer film at solid–liquid interfaces

M. Fukuoka, Y. Takashima, K. Akamatsu, A. Demessence, T. Tsuruoka

CrystEngComm 26 6269 - 6273 2024

Preparation of golf ball-like polymer particles bearing site-selective functional groups

T. Morishita, I. Maitani, T. Michiura, N. Suzuki, T. Suzuki, K. Akamatsu, H. Minami

ACS Appl. Mater.Interfaces in press 2024

Structure and Thermoelectric Characterization of p-Type SnTe Nanobulk Material Synthesized by Charge Compensation Chemical Reaction

Ryosuke Fujiwara, Yuta Ikeda, Takuto Kawaguchi, Yohei Takashima , Takaaki Tsuruoka and Kensuke Akamatsu

Energies 17 ( 190 ) 2024

Cr(NO3)3 as a New Etching Reagent for an Al-based Metal-Organic Framework to Control Its Crystal Size and Defects

Y. Takashima, N. Tanabe, S. Tanaka, T. Tsuruoka, K. Akamatsu

Cryst. Growth Des 24 1766 - 1773 2024

Direct Formation of ZIF-8 Crystal Thin Films on the Surface of a Zinc Ion-Doped Polymer Substrate

T. Tsuruoka, K. Araki, K. Kawauchi, Y. Takashima, K. Akamatsu

Inroganics 12 ( 21 ) 2024

Interfacial analysis of the ion-transport process controlling the steady-state current in a two-phase electrodeposition system using polyelectrolyte membranes Reviewed

Shunsuke Yamada, Yohei Takashima, Takaaki Tsuruoka, Kensuke Akamatsu

RSC Applied Interfaces 2024

Publisher：Royal Society of Chemistry (RSC)

An interfacial model has been developed for describing ion transport kinetics at the membrane/solution interface in the new solid-electrodeposition system.

DOI： 10.1039/d4lf00112e

Engineering of CdS-chain arrays assembled through S⋯S interactions in 1D semiconductive coordination polymers Reviewed

Asuka Nishibe, Ryohei Akiyoshi, Akinori Saeki, Kazuyoshi Ogasawara, Takaaki Tsuruoka, Daisuke Tanaka

Chemical Communications 2024

Publisher：Royal Society of Chemistry (RSC)

The impact of inorganic (–Cd–S–)n assemblies on semiconductive properties is demonstrated by systematically synthesizing one-dimensional Cd(ii) benzenethiolate coordination polymers.

DOI： 10.1039/d3cc05689a

Structure and Thermoelectric Characterization of p-Type SnTe Nanobulk Material Synthesized by Charge Compensation Chemical Reaction Reviewed

Ryosuke Fujiwara, Yuta Ikeda, Takuto Kawaguchi, Yohei Takashima, Takaaki Tsuruoka, Kensuke Akamatsu

Energies 17 ( 1 ) 190 - 190 2023.12

Publisher：MDPI AG

SnTe is the most widely studied p-type thermoelectric (TE) alternative to PbTe. In this study, we prepared a nanostructured SnTe bulk material via spark plasma sintering from a precursor synthesized by a chemical precipitation process without using organic molecules. The sintered sample comprised tiny grains (100–300 nm) with high-density grain boundaries. Eventually, because the material would contain no impurities acting as scattering nodes of charge carriers, the material exhibited a relatively high electrical conductivity of 7.07 × 105 Sm−1 at 310 K. The material demonstrated low lattice thermal conductivity (0.87 Wm−1K−1 at 764 K), which can be owing to the increasing phonon scattering at grain boundaries. The maximum ZT was 0.31 at 764 K in the measured temperature range. This study provides a method for the design of phase-pure and surfactant-free SnTe thermoelectric materials that exhibit low lattice thermal conductivity and high carrier mobility using a chemical synthetic approach.

DOI： 10.3390/en17010190

Loss of p53 function promotes DNA damage-induced formation of nuclear actin ﬁlaments Reviewed

Torii T, Sugimoto W, Itoh K, Kinoshita N, Gessho M, Goto T, Uehara I, Nakajima W, Budirahardja Y, Miyoshi D, Nishikata T, Tanaka N, Kawauchi K

Cell Death and Disease 14 766 2023.11

Joint Work

DOI： doi.org/10.1038/s41419-023-06310-0

Comparison of evolutionary relationships between Branchiostoma ﬂoridae, Ciona intestinalis, and Homo sapiens globins provide evidence of gene co-option and convergent evolution Reviewed

Yano N, Minamoto T, Yamaguchi H, Goto T, Nishikata T

International Journal of Molecular Science 24 16009 2023.11

Joint Work

Authorship：Last author,　Corresponding author

DOI： doi.org/10.3390/ijms242116009

Sequencing of Morpholino Antisense Oligonucleotides Using Electron Capture Dissociation Mass Spectrometry Reviewed International journal

Kaoru Karasawa, Eva Duchoslav, Lyle Burton, Junji Kawakami, Takashi Baba

Analytical Chemistry 95 ( 44 ) 16352 - 16358 2023.10

Joint Work

We report the first sequencing of morpholino antisense oligonucleotides (phosphorodiamidate morpholino oligomers, PMOs) using electron capture dissociation (ECD) mass spectrometry. In this research, we found dissociation of the backbone of 18- to 25-mer PMOs to produce d and z ions as the major ions, and 100% cleavage coverage (sequence coverage) was obtained with these ions. This is a critical contrast with beam-type collision-induced dissociation, which dominantly induces base loss, so it is difficult to obtain sequence information. The results showed that an electron beam energy (typically 15 eV) can be used universally for PMOs with different sequences, lengths, and charge states so that no detailed optimization is required for multiprecursor targeting liquid chromatography coupled with tandem mass spectrometry measurements. We also confirmed that the ECD reaction speed was compatible with the high-performance liquid chromatography time scale. Finally, we demonstrated a liquid chromatography electron capture dissociation tandem mass spectrometry workflow to survey the modification sites of the emulated PMO impurities.

DOI： 10.1021/acs.analchem.3c03621

Other Link： https://doi.org/10.1021/acs.analchem.3c03621

Mitochondrial Dynamics of Bcl-2 Family Proteins during 17-β-Estradiol-Induced Apoptosis Correlate with the Malignancy of Endometrial Cancer Cells Reviewed

Takahiro Yaguchi, Misaki Kameno, Hirofumi Taira, and Junji Kawakami

Biochemstry 62 ( 21 ) 3041 - 3049 2023.10

DOI： 10.1021/acs.biochem.3c00064

Other Link： https://pubs.acs.org/doi/10.1021/acs.biochem.3c00064

Comparison of Analysis Data of Model Oligonucleotide Therapeutics Obtained with Different Types of Liquid Chromatograph- Mass Spectrometers Reviewed

Kenji Hirose, Tokuyuki Yoshida, Maki Terasaki, Hiroshi Sezaki, Kaoru Karasawa, Noriyuki Iwasaki, Kentaro Takahara, Naomi Takiguchi, Mitsuaki Sekiguchi, Hirokazu Nankai, Emi Saito, Hideaki Sato, Takashi Osawa, Takao Yamaguchi, Kosuke Ito, Junji Kawakami, Satoshi Obika, and Takao Inoue

Pharmaceutical and Medical Device Regulatory Science 54 439 - 454 2023.10

Loss of p53 function promotes DNA damage-induced formation of nuclear actin filaments Reviewed International journal

Takeru Torii, Wataru Sugimoto, Katsuhiko Itoh, Natsuki Kinoshita, Masaya Gessho, Toshiyuki Goto, Ikuno Uehara, Wataru Nakajima, Yemima Budirahardja, Daisuke Miyoshi, Takahito Nishikata, Nobuyuki Tanaka, Hiroaki Hirata, Keiko Kawauchi

Cell Death & Disease 14 ( 11 ) 766766 - 766 2023.8

Tumor suppressor p53 plays a central role in response to DNA damage. DNA-damaging agents modulate nuclear actin dynamics, influencing cell behaviors; however, whether p53 affects the formation of nuclear actin filaments remains unclear. In this study, we found that p53 depletion promoted the formation of nuclear actin filaments in response to DNA-damaging agents, such as doxorubicin (DOXO) and etoposide (VP16). Even though the genetic probes used for the detection of nuclear actin filaments exerted a promotive effect on actin polymerization, the detected formation of nuclear actin filaments was highly dependent on both p53 depletion and DNA damage. Whilst active p53 is known to promote caspase-1 expression, the overexpression of caspase-1 reduced DNA damage-induced formation of nuclear actin filaments in p53-depleted cells. In contrast, co-treatment with DOXO and the pan-caspase inhibitor Q-VD-OPh or the caspase-1 inhibitor Z-YVAD-FMK induced the formation of nuclear actin filament formation even in cells bearing wild-type p53. These results suggest that the p53-caspase-1 axis suppresses DNA damage-induced formation of nuclear actin filaments. In addition, we found that the expression of nLifeact-GFP, the filamentous-actin-binding peptide Lifeact fused with the nuclear localization signal (NLS) and GFP, modulated the structure of nuclear actin filaments to be phalloidin-stainable in p53-depleted cells treated with the DNA-damaging agent, altering the chromatin structure and reducing the transcriptional activity. The level of phosphorylated H2AX (γH2AX), a marker of DNA damage, in these cells also reduced upon nLifeact-GFP expression, whilst details of the functional relationship between the formation of nLifeact-GFP-decorated nuclear actin filaments and DNA repair remained to be elucidated. Considering that the loss of p53 is associated with cancer progression, the results of this study raise a possibility that the artificial reinforcement of nuclear actin filaments by nLifeact-GFP may enhance the cytotoxic effect of DNA-damaging agents in aggressive cancer cells through a reduction in gene transcription.

DOI： 10.1038/s41419-023-06310-0

PubMed

Effect of linearly polarized microwaves on nanomorphology of calcium carbonate mineralization using peptides Reviewed

Kenji Usui, Makoto Ozaki, Kan Hirao, Tsubasa Kosaka, Natsumi Endo, Shuhei Yoshida, Shin-ichiro Yokota, Yonejiro Arimoto, Ryuji Osawa, Nobuhiro Nakanishi, Kin-ya Tomizaki, Tomohiro Umetani, Fumihiro Kayamori

Scientific Reports 13 12027 2023.7

Authorship：Last author,　Corresponding author

Microwaves are used for diverse applications such as mobile phones, ovens, and therapy devices. However, there are few reports on the effects of microwaves on diseases other than cancer, and on physiological processes. Here, we focused on CaCO3 mineralization as a model of biomineralization and attempted to elucidate the effect of microwaves on CaCO3 mineralization using peptides. We conducted AFM, ζ potential, HPLC, ICP-AES, and relative permittivity measurements. Our findings show that microwaves alter the nanomorphology of the CaCO3 precipitate, from sphere-like particles to string-like structures. Furthermore, microwaves have little effect on the mineralization when the mineralization ability of a peptide is high, but a large effect when the precipitation ability is low. Our findings may be applicable to not only the treatment of teeth and bones but also the development of organic–inorganic nanobiomaterials. This methodology can be expanded to other molecular/atomic reactions under various microwave conditions to alter reaction activity parameters.

Thixotropic β-Chitin Nanofiber Hydrogel with a Living Body-Responsive Self-Hardening Property and Its Application as a Sprayable Antiadhesion Barrier Reviewed International journal

Koji Nagahama, Kaho Tobiishi, Natsumi Ueda, Tatsuya Dode, Shahidul Islam Sobuj, Fumiya Yuasa, Kazuhiko Yamashita, Takahito Nishikata

ACS Applied Bio Materials 6 ( 7 ) 2636 - 2643 2023.7

Authorship：Lead author,　Corresponding author

DOI： doi.org/10.1021/acsabm.3c00221

Effect of linearly polarized microwaves on nanomorphology of calcium carbonate mineralization using peptides Reviewed

Scientific Reports 13 12027 2023.7

Inhibitory Effect of Adsorption of Streptococcus mutans onto Scallop-Derived Hydroxyapatite Reviewed

Momoko Usuda, Mariko Kametani, Masakazu Hamada, Yuto Suehiro, Saaya Matayoshi, Rena Okawa, Shuhei Naka, Michiyo Matsumoto-Nakano, Tatsuya Akitomo, Chieko Mitsuhata, Kazuya Koumoto, Keiko Kawauchi, Takahito Nishikata, Masatoshi Yagi, Toshiro Mizoguchi, Koki Fujikawa, Taizo Taniguchi, Kazuhiko Nakano and Ryota Nomura

Int J Mol Sci 24 11371 2023.7

Inhibitory Effect of Adsorption of Streptococcus mutans onto Scallop-Derived Hydroxyapatite Reviewed

Usuda M, Kametani M, Hamada M, Suehiro Y, Matayoshi S, Okawa R, Naka S, Matsumoto-Nakano M, Akitomo T, Mitsuhata C, Koumoto K, Kawauchi K, Nishikata T, Yagi M, Mizoguchi T, Fujikawa K, Taniguchi T, Nakano K, Nomura R

International Journal of Molecular Science 24 11371 2023.7

Joint Work

DOI： doi.org/10.3390/ijms241411371

Inhibitory Effect of Adsorption of Streptococcus mutans onto Scallop-Derived Hydroxyapatite. Reviewed International journal

International journal of molecular sciences 24 ( 14 ) 2023.7

Hydroxyapatite adsorbs various substances, but little is known about the effects on oral bacteria of adsorption onto hydroxyapatite derived from scallop shells. In the present study, we analyzed the effects of adsorption of Streptococcus mutans onto scallop-derived hydroxyapatite. When scallop-derived hydroxyapatite was mixed with S. mutans, a high proportion of the bacterial cells adsorbed onto the hydroxyapatite in a time-dependent manner. An RNA sequencing analysis of S. mutans adsorbed onto hydroxyapatite showed that the upregulation of genes resulted in abnormalities in pathways involved in glycogen and histidine metabolism and biosynthesis compared with cells in the absence of hydroxyapatite. S. mutans adsorbed onto hydroxyapatite was not killed, but the growth of the bacteria was inhibited. Electron microscopy showed morphological changes in S. mutans cells adsorbed onto hydroxyapatite. Our results suggest that hydroxyapatite derived from scallop shells showed a high adsorption ability for S. mutans. This hydroxyapatite also caused changes in gene expression related to the metabolic and biosynthetic processes, including the glycogen and histidine of S. mutans, which may result in a morphological change in the surface layer and the inhibition of the growth of the bacteria.

DOI： 10.3390/ijms241411371

PubMed

Thixotropic β‑Chitin Nanofiber Hydrogel with a Living BodyResponsive Self-Hardening Property and Its Application as a Sprayable Antiadhesion Barrier Reviewed

Nagahama K, Tobiishi K, Ueda N, Dode T, Sobuj SI, Yuasa F, Yamashita K, Nishikata T

American Chemical Society Applied Bio Materials 2023 ( 6 ) 2636 - 2643 2023.7

Joint Work

Authorship：Last author

DOI： 10.1021/acsabm.3c00221

Co-generation of palladium nanoparticles and phosphate supported on metal-organic frameworks as hydrogenation catalysts Reviewed

Yohei Takashima, Seiko Tetsusashi, Mai Takano, Shintaro Tanaka, Yui Murakami, Takaaki Tsuruoka, Kensuke Akamatsu

Dalton Trans. 52 11158 - 11162 2023.7

Authorship：Lead author,　Corresponding author

Other Link： https://pubs.rsc.org/en/content/articlelanding/2023/dt/d2dt04109j

The iron chelator deferriferrichrysin induces paraptosis via extracellular signal-related kinase activation in cancer cells Invited Reviewed

Natsuki Kinoshita, Masaya Gessho, Takeru Torii, Yukako Ashida, Minori Akamatsu, Alvin Kunyao Guo, Sunmin Lee, Tatsuya Katsuno 3, Wataru Nakajima Yemima Budirahardja, Daisuke Miyoshi, Takehiko Todokoro, Hiroki Ishida, Takahito Nishikata, Keiko Kawauchi

Genes Cells 2023.6

DOI： 10.1111/gtc.13053

Quantification of the concentration in a droplet formed by liquid–liquid phase separation of G-quadruplex-forming RNA Reviewed

Kohei Yokosawa, Mitsuki Tsuruta, Shinji Kajimoto, Naoki Sugimoto, Daisuke Miyoshi, Takakazu Nakabayashi

Chem. Phys. Lett. 826 140634 - 140634 2023.6

Publisher：Elsevier BV

DOI： 10.1016/j.cplett.2023.140634

The iron chelator deferriferrichrysin induces paraptosis via extracellular-signal-regulated kinase activation in cancer cells Reviewed International coauthorship

Kinoshita N, Gessho M, Torii T, Ashida Y, Akamatsu M, Guo AK, Lee S, Katsuno T, Nakajima W, Budirahardja Y, Miyoshi D, Todokoro T, Ishida H, Nishikata T, Kawauchi K

Genes to Cells 28 ( 9 ) 653 - 662 2023.6

Authorship：Last author,　Corresponding author

DOI： doi.org/10.1111/gtc.13053

Simple and fast screening for structure-selective G-quadruplex ligands. Reviewed International journal

Yoshiki Hashimoto, Yoshiki Imagawa, Kaho Nagano, Ryuichi Maeda, Naho Nagahama, Takeru Torii, Natsuki Kinoshita, Nagisa Takamiya, Keiko Kawauchi, Hisae Tatesishi-Karimata, Naoki Sugimoto, Daisuke Miyoshi

Chemical communications (Cambridge, England) 59 ( 33 ) 4891 - 4894 2023.4

Joint Work

Structural selectivity of G-quadruplex ligands is essential for cellular applications since there is an excess of nucleic acids forming duplex structures compared to G-quadruplex structures in living cells. In this study, we developed new structure-selective G-quadruplex ligands utilizing a simple and fast screening system. The affinity, selectivity, enzymatic inhibitory activity and cytotoxicity of the structure-selective G-quadruplex ligands were demonstrated along with a structural selectivity-cytotoxicity relationship of G-quadruplex ligands.

DOI： 10.1039/d3cc00556a

PubMed

Not all 2',4'-bridged modifications stabilize DNA/RNA duplexes Reviewed

Tomoka Akita, Elisa Tomita-Sudo, Shin Itoh, Nae Sakimoto, Takeshi Masuda, Akifumi Nakamura, Yoshiyuki Onishi, Makoto Koizumi Junji Kawakami

Nucleosides, Nucleotides & Nucleic Acids 2023.3

Joint Work

2’,4’-Bridged modifications such as 2’-O,4’-C-methylene-bridged nucleotides (LNAs) and 2’-O,4’-C-ethylene-bridged nucleotides (ENAs) provide high binding affinity for duplex formation. Stabilization by the introduction of the bridged nucleic acids is considered to be due to pre-organization. In this study, we found that the introduction of 2’,4’-C-bridged 2’-deoxynucleotides (CRNs; Conformationally Restricted Nucleotides) into DNA/RNA duplexes leads to destabilization, as opposed to the previously accepted notion that 2’,4’-bridged modifications always lead to stabilization.

Other Link： https://www.tandfonline.com/doi/full/10.1080/15257770.2023.2232414

Biomolecular Liquid–Liquid Phase Separation for Biotechnology Invited Reviewed

Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi and Daisuke Miyoshi

BioTech 12 ( 2 ) 26 2023.3

Authorship：Corresponding author

DOI： 10.3390/biotech12020026

Construction of a Method to Design Fibril-Forming Peptides Applied to Biomaterials from 20 Beta-Sheet Peptides by Statistical Analysis Reviewed

Kazuya Iwata, Taisei Terao, Akira Takekawa, Tomohiro Umetani, Kenji Usui

Peptide Science 2022 141 - 142 2023.3

Authorship：Last author,　Corresponding author

High-performance bulk Bi0.4Sb1.6Te3.0 thermoelectrics prepared from nanocrystal precursor synthesized via chemical precipitation Reviewed

Ryosuke Fujiwara, Yohei Takashima, Takaaki Tsuruoka, Muneyuki Naito, Junya Murai, Kensuke Akamatsu

Journal of Solid State Chemistry 319 123777 - 123777 2023.3

Publisher：Elsevier BV

DOI： 10.1016/j.jssc.2022.123777

Direct generation of polypyrrole-coated palladium nanoparticles inside a metal-organic framework for a semihydrogenation catalyst Reviewed

Yohei Takashima, Seiko Tetsusashi, Shintaro Tanaka, Takaaki Tsuruoka, Kensuke Akamatsu

RSC Adv. 13 7464 - 7467 2023.3

Authorship：Lead author

Other Link： https://pubs.rsc.org/en/content/articlelanding/2023/ra/d2ra08190c

Simple and fast screening for structure-selective G-quadruplex ligands Reviewed International journal

Chem. Commun. 59 ( 33 ) 4891 - 4894 2023.2

Authorship：Corresponding author

DOI： 10.1039/D3CC00556A

PubMed

Morphological Evidence for Novel Roles of Microtubules in Macrophage Phagocytosis Reviewed

Seta Y, Kawakatsu K, Degawa S, Goto T, Nishikata T

International Journal of Molecular Science 24 1373 2023.1

Joint Work

Authorship：Last author,　Corresponding author

DOI： doi.org/10.3390/ijms24021373

RNA 高次構造を標的とした光線力学療法の展望 Invited

取井猛流・木下菜月・橋本佳樹・杉本渉・川内敬子・三好大輔

38 ( 13 ) 1154 - 1156 2023

Authorship：Last author,　Corresponding author

グアニン四重らせん構造による転移因子LINE-1の発現抑制名

月生雅也, 李先民, Yemima Suryani Budirahardja, 鶴田充生, 橋本佳樹, 高宮渚, 木下菜月, 建石寿枝, 杉本直己, 三好大輔, 川内敬子

日本女性科学者の会学術誌 23 48 - 48 2023

Publisher：(一社)日本女性科学者の会

High-performance bulk Bi0.4Sb1.6Te3.0 thermoelectrics prepared from nanocrystal precursor synthesized via chemical precipitation

R. Fujiwara, Y.Takashima, T. Tsuruoka, M. Naito, J. Murai, K. Akamatsu

Journal of Solid State Chemistry 319 123777 2023

“Direct generation of polypyrrole-coated palladium nanoparticles inside a metal-organic framework for a semihydrogenation catalyst

Y. Takashima, S. Tetsusashi, S. Tanaka, T. Tsuruoka, K. Akamatsu

RSC Adv 13 7464 - 7467 2023

“Co-generation of palladium nanoparticles and phosphate supported on metal-organic frameworks as hydrogenation catalysts

Y. Takashima, S. Tetsusashi, M. Takano, S. Tanaka, Y. Murakami, T. Tsuruoka, K. Akamatsu

Dalton Trans 52 11158 - 11162 2023

Direct generation of polypyrrole-coated palladium nanoparticles inside a metal-organic framework for a semihydrogenation catalyst Reviewed

Yohei Takashima, Seiko Tetsusashi, Shintaro Tanaka, Takaaki Tsuruoka, Kensuke Akamatsu

RSC Advances 13 ( 11 ) 7464 - 7467 2023

Publisher：Royal Society of Chemistry (RSC)

Polypyrrole-coated palladium nanoparticles could be directly generated inside a metal–organic frameworks. The resulting MOF composites exhibited higher semihydrogenation capability than the analog composite without polypyrrole coating.

DOI： 10.1039/d2ra08190c

Co-generation of palladium nanoparticles and phosphate supported on metal–organic frameworks as hydrogenation catalysts Reviewed

Yohei Takashima, Seiko Tetsusashi, Mai Takano, Shintaro Tanaka, Yui Murakami, Takaaki Tsuruoka, Kensuke Akamatsu

Dalton Transactions 52 ( 32 ) 11158 - 11162 2023

Publisher：Royal Society of Chemistry (RSC)

In this study, we demonstrated the direct synthesis of sodium dihydrogen phosphate (PA) containing palladium nanoparticles (PdNPs) supported on a metal–organic framework (MOF) for hydrogenation catalysis.

DOI： 10.1039/d2dt04109j

Evaluation of thermal stability of DNA oligonucleotide structures embedded in hydrogels Invited Reviewed International journal

D. Yamaguchi, M. Yoshida, S. Nakano

DNA 2 ( 4 ) 302 - 313 2022.12

Joint Work

Authorship：Last author,　Corresponding author Publisher：MDPI

Understanding the self-assembly and hybridization properties of DNA oligonucleotides in confined spaces can help to improve their applications in biotechnology and nanotechnology. This study investigates the effects of spatial confinement in the pores of hydrogels on the thermal stability of DNA oligonucleotide structures. The preparation of oligonucleotides embedded in agarose gels was simple, whereas the preparation of oligonucleotides embedded in polyacrylamide gels was required to remove unpolymerized monomers. In the latter case, a method for rehydrating a washed dry gel with a buffer solution containing oligonucleotides was developed. Fluorescence measurements of oligonucleotides bearing fluorescent probes revealed no significant influence of the internal environment of the gel pores on the stability of DNA duplex, hairpin, and G-quadruplex structures. Moreover, the effects of poly(ethylene glycol) on the stability of DNA structures in the gels were similar to those in solutions. It is likely that the oligonucleotides are not strongly constrained in the gels and may be preferentially located in a water-rich environment in the gel matrix. The gel preparation was also applied to the assessment of the stability of DNA structures under the conditions of a reduced number of water molecules. The studies using hydrogels provide insights into the ability of self-assembly and hybridization of oligonucleotides in confined environments and under low-water-content conditions.

DOI： 10.3390/dna2040021

Controlling liquid-liquid phase separation of G-quadruplex-forming RNAs in a sequence-specific manner. Reviewed International journal

Mitsuki Tsuruta, Takeru Torii, Kazuki Kohata, Keiko Kawauchi, Hisae Tateishi-Karimata, Naoki Sugimoto, Daisuke Miyoshi

Chemical communications (Cambridge, England) 58 ( 93 ) 12931 - 12934 2022.11

Joint Work

We constructed a minimum liquid-liquid phase separation model system to form liquid droplets using only G-quadruplex-forming oligonucleotides and R- and G-rich oligopeptides. We found that the G-quadruplex structure is an essential component for RNA to form droplets with the peptide. Based on this model system and our findings, droplet redissolution via structure transition from a G-quadruplex to a duplex was achieved in a sequence-specific manner.

DOI： 10.1039/d2cc04366a

PubMed

Covalent Stem Cell-Combining Injectable Materials with Enhanced Stemness and Controlled Differentiation In Vivo Reviewed

Natsumi Ueda, Shiho Sawada, Fumiya Yuasa, Karen Kato, Koji Nagahama

ACS Applied Materials & Interfaces 14 ( 47 ) 52618 - 52633 2022.11

Authorship：Last author,　Corresponding author

DOI： https://doi.org/10.1021/acsami.2c12918

ポリエチレングリコールによるアトピー性皮膚炎予防の可能性

厳原美穂、甲元一也

Fragrance Journal 50 ( 11 ) 54 - 57 2022.11

Controlling liquid–liquid phase separation of G-quadruplex-forming RNAs in a sequence-specific manner Reviewed International journal

Mitsuki Tsuruta, Takeru Torii, Kazuki Kohata, Keiko Kawauchi, Hisae Tateishi-Karimata, Naoki Sugimoto and Daisuke Miyoshi

Chem. Commun. 58 ( 93 ) 12931 - 12934 2022.11

DOI： 10.1039/D2CC04366A

PubMed

Two-Dimensional Metal–Organic Framework-Based Cellular Scaffolds with High Protein Adsorption, Retention, and Replenishment Capabilities Reviewed

Tokitaka Katayama, Shintaro Tanaka, Takaaki Tsuruoka, and Koji Nagahama

ACS Applied Materials & Interfaces 14 ( 30 ) 34443 - 34454 2022.8

Authorship：Last author,　Corresponding author

Two-Dimensional Metal–Organic Framework-Based Cellular Scaffolds with High Protein Adsorption, Retention, and Replenishment Capabilities Reviewed

Tokitaka Katayama, Shintaro Tanaka, Takaaki Tsuruoka, Koji Nagahama

ACS Applied Materials & Interfaces 2022.8

DOI： 10.1021/acsami.2c08677

Evaluation of weak interactions of proteins and organic cations with DNA duplex structures Reviewed International journal

R. Morimoto, M. Horita, D. Yamaguchi, H. Nakai, and S. Nakano

Biophys. J. 121 ( 15 ) 2873 - 2881 2022.8

Joint Work

Authorship：Last author,　Corresponding author Publisher：Cell Press

Molecular interactions and reactions in living cells occur with high background concentrations of organic compounds including proteins. Uncharged water-soluble polymers are commonly used cosolutes in studies on molecular crowding, and most studies argue about the effects of intracellular crowding based on results obtained using polymer cosolutes. Further investigations using protein crowders and organic cations are important in understanding the effects of cellular environments on nucleic acids with negatively charged surfaces. We assessed the effects of using model globular proteins, serum proteins, histone proteins, structurally flexible polypeptides, di- and polyamines, and uncharged polymers. Thermal stability analysis of DNA oligonucleotide structures revealed that unlike conventional polymer cosolutes, basic globular proteins (lysozyme and cytochrome c) at high concentrations stabilized long internal and bulge loop structures but not fully matched duplexes. The selective stabilization of long loop structures suggests preferential binding to unpaired nucleotides in loops through weak electrostatic interactions. Furthermore, the ability of the proteins to stabilize the loop structures was enhanced under macromolecular crowding conditions. Remarkably, the effects of basic proteins on the stability of fully matched duplexes were dissimilar to those of basic amino-acid-rich polypeptides and polyamines. This study provides new insights into the interaction of nucleic acid structures with organic cations.

DOI： 10.1016/j.bpj.2022.07.003

細胞内環境で安定化される核酸非構造を狙った分子標的薬の開発 Invited

川内敬子・橋本佳樹・杉本　渉・三好大輔

バイオマテリアル = Journal of Japanese Society for Biomaterials : 生体材料 40 ( 3 ) 206 - 211 2022.7

Authorship：Corresponding author

核酸の化学修飾と構造や相分離能の相関 Invited

鶴田充生・取井猛流・杉本渉・川内敬子・三好大輔

月刊「細胞」（ニューサイエンス社） 53 ( 14 ) 910 - 913 2022.6

Authorship：Last author,　Corresponding author

Exportin-inspired artificial cell nuclear-exporting nanosystems Reviewed

Mitsuo Inui , Yuta Hamada , Nana Sejima , Natsumi Ueda , Tokitaka Katayama , Kimika Ono, Koji Nagahama

Nanoscale Advances 4 2637 - 2641 2022.5

Authorship：Last author,　Corresponding author

Significant structural change in human c-Myc promoter G-quadruplex Reviewed International coauthorship

Nikita Kundu, Taniya Sharma, Sarvpreet Kaur, Mamta Singh, Vinit Kumar, Uttam Sharma, Aklank Jain, Jadala Shankaraswamy, Daisuke Miyoshi, Sarika Saxena

RSC Adv. 12 7594 - 7604 2022.4

Angiogenesis Promotion by Combined Administration of DFO and Vein Endothelial Cells Using Injectable, Biodegradable, Nanocomposite Hydrogel Scaffolds Reviewed

Kimika Ono, Manami Sumiya, Naohiro Yoshinobu, Tatsuya Dode, Tokitaka Katayama, Natsumi Ueda, Koji Nagahama

ACS Applied Bio Materials 5 ( 2 ) 471 - 482 2022.2

Authorship：Last author,　Corresponding author

Actin Filament in the First Cell Cycle Contributes to the Determination of the Anteroposterior Axis in Ascidian Development Reviewed

Toshiyuki Goto, Shuhei Torii, Aoi Kondo, Kazumasa Kanda, Junji Kawakami, Yosky Kataoka, Takahito Nishikata

Journal of Developmental Biology 10 ( 1 ) 10 - 10 2022.2

Publisher：MDPI AG

In many animal species, the body axis is determined by the relocalization of maternal determinants, organelles, or unique cell populations in a cytoskeleton-dependent manner. In the ascidian first cell cycle, the myoplasm, including mitochondria, endoplasmic reticulum (ER), and maternal mRNAs, move to the future posterior side concomitantly (called ooplasmic segregation or cytoplasmic and cortical reorganization). This translocation consists of first and second phases depending on the actin and microtubule, respectively. However, the transition from first to second phase, that is, translocation of myoplasmic components from microfilaments to microtubules, has been poorly investigated. In this study, we analyzed the relationship between these cytoskeletons and myoplasmic components during the first cell cycle and their role in morphogenesis by inhibitor experiments. Owing to our improved visualization techniques, there was unexpected F-actin accumulation at the vegetal pole during this transition period. When this F-actin was depolymerized, the microtubule structure was strongly affected, the myoplasmic components, including maternal mRNA, were mislocalized, and the anteroposterior axis formation was disordered. These results suggested the importance of F-actin during the first cell cycle and the existence of interactions between microfilaments and microtubules, implying the enigmatic mechanism of ooplasmic segregation. Solving this mystery leads us to an improved understanding of ascidian early development.

DOI： 10.3390/jdb10010010

Dynamic Changes in the Aassociation between Maternal mRNAs and Endoplasmic Reticulum during Ascidian Early Embryogenesis. Reviewed

Goto T, Torii S, Kondo A, Yagi H, Suekane M, Kataoka Y, Nishikata T

Development Genes and Evolution 232 ( 1 ) 1 - 14 2022.2

Joint Work

Authorship：Last author,　Corresponding author

DOI： 10.1007/s00427-021-00683-y

Actin Filament in the First Cell Cycle Contributes to the Determination of the Anteroposterior Axis in Ascidian Development. Reviewed

Goto T, Torii S, Kondo A, Kanda K, Kataoka Y, Nishikata T

Journal of Developmental Biology 10 ( 1 ) 10 - 23 2022.2

Joint Work

Authorship：Last author,　Corresponding author

DOI： 10.3390/jdb10010010

Intramolecular G-quadruplex-hairpin loop structure competition of a GC-rich exon region in the TMPRSS2 gene Reviewed

Chem. Commun. 58 48 - 51 2022.1

Authorship：Corresponding author

De novo design of a nanopore for single-molecule detection that incorporates a β-hairpin peptide. Reviewed International coauthorship International journal

Keisuke Shimizu, Batsaikhan Mijiddorj, Masataka Usami, Ikuro Mizoguchi, Shuhei Yoshida, Shiori Akayama, Yoshio Hamada, Akifumi Ohyama, Kenji Usui, Izuru Kawamura, Ryuji Kawano

Nature nanotechnology 17 67 - 75 2022.1

The amino-acid sequence of a protein encodes information on its three-dimensional structure and specific functionality. De novo design has emerged as a method to manipulate the primary structure for the development of artificial proteins and peptides with desired functionality. This paper describes the de novo design of a pore-forming peptide, named SV28, that has a β-hairpin structure and assembles to form a stable nanopore in a bilayer lipid membrane. This large synthetic nanopore is an entirely artificial device for practical applications. The peptide forms multidispersely sized nanopore structures ranging from 1.7 to 6.3 nm in diameter and can detect DNAs. To form a monodispersely sized nanopore, we redesigned the SV28 by introducing a glycine-kink mutation. The resulting redesigned peptide forms a monodisperse pore with a diameter of 1.7 nm leading to detection of a single polypeptide chain. Such de novo design of a β-hairpin peptide has the potential to create artificial nanopores, which can be size adjusted to a target molecule.

DOI： 10.1038/s41565-021-01008-w

PubMed

Chemical synthesis of single nanometer-sized Bi2−xSbxTe3.0 nanocrystals via direct precipitation process Reviewed

Ryosuke Fujiwara, Yohei Takashima, Takaaki Tsuruoka, Muneyuki Naito, Junya Murai, Kensuke Akamatsu

Results in Chemistry 4 100485 - 100485 2022.1

Publisher：Elsevier BV

DOI： 10.1016/j.rechem.2022.100485

Intramolecular G-quadruplex-hairpin loop structure competition of a GC-rich exon region in the TMPRSS2 gene

Wataru Sugimoto, Natsuki Kinoshita, Minori Nakata, Tatsuya Ohyama, Hisae Tateishi-Karimata, Takahito Nishikata, Naoki Sugimoto, Daisuke Miyoshi, Keiko Kawauchi

Chemical Communications 58 ( 1 ) 48 - 51 2022

Publisher：Royal Society of Chemistry (RSC)

We identified cytosine-rich regions adjacent to guanine-rich regions in the TMPRSS2 gene, which showed structural competition between a G-quadruplex and a hairpin loop. Furthermore, this competition significantly affected transcription efficiency.

DOI： 10.1039/d1cc05523b

核酸の四重らせん構造を狙った治療薬の展望 Invited

37 ( 4 ) 52 - 55 2022

Authorship：Corresponding author

rDNAおよびrRNAが形成する四重らせん構造による核小体機能の制御 Invited

川内敬子、取井猛流、月生雅也、木下菜月、三好大輔

月刊細胞 54 ( 12 ) 36 - 39 2022

Authorship：Corresponding author

Rational and site-selective formation of coordination polymer consisiting of d10 coinage metal ions with thiolate ligands using metal ion-doped polymer substrate

T. Tsuruoka, Y. Miyashita, R. Yoshino, M. Fukuoka, S. Hirao, Y. Takashima, A. Demessence, K. Akamatsu

RSC Adv 12 3716 - 3720 2022

Chemical synthesis of single nanometer-sized Bi2-xSbxTe3.0 nanocrystals via direct precipitation process

R. Fujiwara, Y.Takashima, T. Tsuruoka, M. Naito, J. Murai, K. Akamatsu

Chemistry 4 100485 2022

ポリイミド基板上への選択的金属有機構造体の形成技術

福岡美海、江口慧一、髙嶋洋平、赤松謙祐、鶴岡孝章

ポリイミドの高機能設計と応用技術 6 ( 6 ) 487 - 495 2022

Cooperative catalysis in a metal–organic framework via post-synthetic immobilisation Reviewed

Yohei Takashima, Yuya Suwaki, Takaaki Tsuruoka, Kensuke Akamatsu

Dalton Transactions 51 ( 24 ) 9229 - 9232 2022

Publisher：Royal Society of Chemistry (RSC)

A Co-salen complex and a MOF were successfully hybridized by post-synthetic immobilization for a cooperative catalytic system.

DOI： 10.1039/d2dt01544g

Rational and site-selective formation of coordination polymers consisting of d10 coinage metal ions with thiolate ligands using a metal ion-doped polymer substrate Reviewed International coauthorship

Takaaki Tsuruoka, Yuri Miyashita, Ryuki Yoshino, Myu Fukuoka, Shoya Hirao, Yohei Takashima, Aude Demessence, Kensuke Akamatsu

RSC Advances 12 ( 6 ) 3716 - 3720 2022

Authorship：Lead author,　Corresponding author Publisher：Royal Society of Chemistry (RSC)

We report an interfacial approach for fabricating coordination polymer (CP) crystal films and patterns on a polymer surface using a metal ion-doped polymer substrate.

DOI： 10.1039/d2ra00269h

ポリイミド基板上への選択的金属有機構造体の形成技術

福岡美海、江口慧一、髙嶋洋平、赤松謙祐、鶴岡孝章

ポリイミドの高機能設計と応用技術 6 ( 6 ) 487 - 495 2022

Authorship：Corresponding author

Rational and site-selective formation of coordination polymer consisiting of d10 coinage metal ions with thiolate ligands using metal ion-doped polymer substrate Reviewed International coauthorship

T. Tsuruoka, Y. Miyashita, R. Yoshino, M. Fukuoka, S. Hirao, Y. Takashima, A. Demessence, K. Akamatsu

RSC Adv 12 3716 - 3720 2022

Anti-Malignant Effect of Tensile Loading to Adherens Junctions in Cutaneous Squamous Cell Carcinoma Cells Reviewed International journal

Dobrokhotov O, Sunagawa M, Torii T, Mii S, Kawauch K, Enomoto A, Sokabe M, Hirata H.

Frontiers in Cell and Developmental Biology 11 2021.11

Joint Work

DOI： doi.org/10.3389/fcell.2021.728383

Injectable Biocatalytic Nanocomposite Hydrogel Factories for Focal Enzyme-Prodrug Cancer Therapy Reviewed

Kimika Ono, Hiroyuki Hashimoto, Tokitaka Katayama, Natsumi Ueda, Koji Nagahama

Biomacromolecules 22 ( 10 ) 4217 - 4227 2021.10

Authorship：Last author,　Corresponding author

Supercapacitor electrode with high charge density based on boron-doped porous carbon derived from covalent organic frameworks Reviewed

Shigeyuki Umezawa, Takashi Douura, Koji Yoshikawa, Yohei Takashima, Mika Yoneda, Kazuma Gotoh, Vlad Stolojan, S. Ravi P. Silva, Yasuhiko Hayashi, Daisuke Tanaka

Carbon 184 418 - 425 2021.10

Publisher：Elsevier BV

DOI： 10.1016/j.carbon.2021.08.022

Biological Tissue-Inspired Living Self-Healing Hydrogels Based on Cadherin-Mediated Specific Cell–Cell Adhesion Reviewed

Koji Nagahama, Seika Aoyama, Natsumi Ueda, Yuka Kimura, Tokitaka Katayama, Kimika Ono

ACS Macro Letters 10 ( 8 ) 1073 - 1079 2021.8

Authorship：Lead author,　Corresponding author

Involvement of Annexin A2 in Serum-MAF Dependent Phagocytic Activation of Macrophages. Reviewed

Kawakatsu K, Seta Y, Maemura M, Nishikata T.

Anticancer Research 41 ( 8 ) 4089 - 4092 2021.8

Joint Work

Authorship：Last author,　Corresponding author

DOI： 10.21873/anticanres.15211

ウォッシャーディスインフェクターのすすぎ排水中に含まれる洗浄剤残留量の測定によるすすぎ性能評価 Reviewed

三軒隼人、藤田敏、川田原瑠勇、武川公、臼井健二、原田陽滋

医療機器学 91 315 - 332 2021.8

Enhancement of the Catalytic Activity of Hammerhead Ribozymes by Organic Cations. Reviewed International journal

Shu-Ichi Nakano, Hirofumi Yamashita, Naoki Sugimoto

ChemBioChem 22 ( 17 ) 2721 - 2728 2021.7

Joint Work

Authorship：Lead author,　Corresponding author

Catalytic turnover is important for the application of ribozymes to biotechnology. However, the turnover is often impaired because of the intrinsic high stability of base pairs with cleaved RNA products. Here, organic cations were used as additives to improve the catalytic performance of hammerhead ribozyme constructs that exhibit different kinetic behaviors. Kinetic analysis of substrate cleavage demonstrated that bulky cations, specifically tetra-substituted ammonium ions containing pentyl groups or a benzyl group, have the ability to greatly increase the turnover rate of the ribozymes. Thermal stability analysis of RNA structures revealed that the bulky cations promote the dissociation of cleaved products and refolding of incorrectly folded structures with small disruption of the catalytic structure. The use of bulky cations is a convenient method for enhancing the catalytic activity of hammerhead ribozymes, and the approach may be useful for advancing ribozyme technologies.

DOI： 10.1002/cbic.202100280

PubMed

Characterization of stable hypoxia-preconditioned dental pulp stem cells compared with mobilized dental pulp stem cells for application for pulp regenerative therapy. Reviewed

Zayed, M., Iohara, K., Watanabe, H., Ishikawa, M., Tominaga, M. and Nakashima, M.

Stem Cell Res Ther. 12 ( 302 ) 2021.5

Detection of Intracellular Reactive Oxidative Species Using the Fluorescent Probe Hydroxyphenyl Fluorescein Invited Reviewed

Wataru Sugimoto, Daisuke Miyoshi, Keiko Kawauchi

Methods in Molecular Biology 2274 207 - 215 2021.5

Publisher：Humana Press Inc.

Various fluorescent probes for the detection of intracellular reactive oxidative species (ROS) have been developed because ROS levels are closely associated with cellular states. Here, we describe a method for detection of intracellular ROS in living cells using the fluorescent probe, hydroxyphenyl fluorescein (HPF), which detects hydroxyl radicals and peroxynitrite. NIH3T3 cells and p53 knockout (p53−/−) mouse embryonic fibroblasts (MEFs) were transformed by expressing oncogenic RAS using a retrovirus system. The cells were treated with HPF at 37 °C for 30 min, and subsequently, images were acquired using a confocal fluorescence microscope at an excitation wavelength of 488 nm after washing with PBS.

DOI： 10.1007/978-1-0716-1258-3_18

PubMed

Molecularly targeted photodynamic therapy for cancer mRNAs Invited Reviewed

杉本渉, 橋本佳樹, 川内敬子, 三好大輔

Bio Clinica 36 ( 5 ) 65 - 67 2021.5

Joint Work

J-GLOBAL

Controlling interfacial ion-transport kinetics using polyelectrolyte membranes for additive- and effluent-free, high-performance electrodeposition Reviewed International journal

K. Akamatsu, S. Nakano, K. Kimura, Y. Takashima, T. Tsuruoka, H. Nawafune, Y. Sato, J. Murai, and H. Yanagimoto

ACS Appl. Mater. Interfaces 13 ( 11 ) 13896 - 13906 2021.3

Joint Work

Publisher：ACS Publications

The development of high-performance, environmentally friendly electrodeposition processes is critical for emerging coating technologies because current technologies use highly complex baths containing metal salts, supporting electrolytes, and various kinds of organic additives, which are problematic from both environmental and cost perspectives. Here, we show that a 200 μm-thin polyelectrolyte membrane sandwiched between electrodes effectively concentrates metal ions through interfacial penetration, which increases the conductance between the electrodes to 0.30 S and realizes solid-state electrodeposition that produces no mist, sludge, or even waste effluent. Both, experimental results and theoretical calculations, reveal that electrodeposition is controlled by ion penetration at the solution/polyelectrolyte interface, providing an intrinsically different ion-transport mechanism to that of conventional diffusion-controlled electrodeposition. The setup, which includes 0.50 mol L–1 copper sulfate and no additives, delivers a maximum current density of 300 mA cm–2, which is nearly fivefold higher than that of a current commercial plating bath containing organic additives.

DOI： 10.1021/acsami.1c03608

核小体ストレスにより形成するrDNA G-quadruplex集合体とその機能

杉本渉, 桑本慎, 建石寿枝, 岩根敦子, 高橋宏隆, 三好大輔, 川内敬子

日本薬学会年会要旨集 141年会 29V05 - am10S 2021.3

Publisher：(公社)日本薬学会

Covalent cell-loading injectable hydrogel scaffold significantly promotes tissue regeneration in vivo compared with a conventional physical cell-loading hydrogel scaffold Reviewed

Yuka Kimura, Seika Aoyama, Natsumi Ueda, Tokitaka Katayama, Kimika Ono, Koji Nagahama

Advanced Biology 5 2000106 2021.2

Joint Work

Hydrostatic Pressure-Regulated Cellular Calcium Responses. Reviewed International journal

Minami Fukuchi, Kotaro Oyama, Hiroaki Mizuno, Akihisa Miyagawa, Kazuya Koumoto, Gaku Fukuhara

Langmuir : the ACS journal of surfaces and colloids 37 ( 2 ) 820 - 826 2021.1

Joint Work

Hydrostatic pressure control has attracted much attention and presents a still challenging objective from mechanobiological viewpoints. Herein, we reveal the calcium entry processes in HeLa cells by means of hydrostatic pressure spectroscopy. The steady-state fluorescence spectral data comprehensively elucidated the factors controlling the outcomes of the hydrostatic pressure-stimulated calcium entry behavior. The present work leads to a new perspective on ion regulations in living cells and an attractive alternative to conventional mechanostimuli.

DOI： 10.1021/acs.langmuir.0c03141

PubMed

Combined Effects of Methylated Cytosine and Molecular Crowding on the Thermodynamic Stability of DNA Duplexes Invited Reviewed

Combined Effects of Methylated Cytosine, Molecular Crowding, on, the Thermodynamic Stability of, DNA Duplexes

Int. J. Mol. Sci. 22 ( 2 ) 947 2021.1

Joint Work

DOI： 10.3390/ijms22020947

Exploration of structural transition phenomenon in flexible metal-organic framework formed on polymer substrate

S. Hirao, R. Hamagami, T. Ohhashi, K. Eguchi, N. Kubo, Y. Takashima, K. Akamatsu, T. Tsuruoka

CrystEngComm 23 8498 - 8505 2021

Precisely Controlled Reproducible Synthesis of Palladium Nanoparticles inside Metal-Organic Frameworks with H2 Gas as Reductant: Effects of Framework Crystallinity and H2 Gas

Y. Takashima, Y. Sato, N.Kubo, T. Tsuruoka, K. Akamatsu

Chem. Lett 50 244 - 247 2021

Joint Work

Controlling Interfacial Ion-Transport Kinetics Using Polyelectrolyte Membranes for Additive- and Effluent-free, High-Performance Electrodeposition Reviewed

Kensuke Akamatsu, Shu-ichi Nakano, Koshi Kimura, Yohei Takashima, Takaaki Tsuruoka, Hidemi Nawafune, Yuki Sato, Junya Murai, Hiroshi Yanagimoto

ACS Applied Materials&Interfaces 2021

Joint Work

Intracellular mineralization of gold nanoparticles using gold ion-binding peptides with cell-penetrating ability Reviewed

Ozaki, M., Yoshida, S., Tsuruoka, T., Usui, K.

Chemical Communications 57 2021

Joint Work

Authorship：Last author,　Corresponding author

DOI： 10.1039/d0cc06117d

Elemental composition control of gold-titania nanocomposites by site-specific mineralization using artificial peptides and DNA Reviewed

Ozaki, M., Imai, T., Tsuruoka, T., Sakashita, S., Tomizaki, K.-Y., Usui, K.

Communications Chemistry 4 2021

Joint Work

Authorship：Last author,　Corresponding author

DOI： 10.1038/s42004-020-00440-8

Exploration of structural transition phenomenon in flexible metal-organic framework formed on polymer substrate Reviewed

Shoya Hirao, Ruho Hamagami, Takashi Ohhashi, Keiichi Eguchi, Neo Kubo, Yohei Takashima, Kensuke Akamatsu, Takaaki Tsuruoka

CrystEngComm 23 ( 48 ) 8498 - 8505 2021

Authorship：Last author,　Corresponding author Publisher：Royal Society of Chemistry (RSC)

We investigate the structural transition of directly formed flexible MOF crystals on a polymer substrate.

DOI： 10.1039/d1ce01383a

Precisely Controlled Reproducible Synthesis of Palladium Nanoparticles inside Metal-Organic Frameworks with H2 Gas as Reductant: Effects of Framework Crystallinity and H2 Gas Reviewed

Y. Takashima, Y. Sato, N.Kubo, T. Tsuruoka, K. Akamatsu

Chem. Lett 50 244 - 247 2021

Joint Work

Intracellular mineralization of gold nanoparticles using gold ion-binding peptides with cell-penetrating ability Reviewed

Makoto Ozaki, Shuhei Yoshida, Takaaki Tsuruoka, Kenji Usui

Chemical Communications 2021

Joint Work

Publisher：Royal Society of Chemistry ({RSC})

DOI： 10.1039/D0CC06117D

Photosensitizers Based on G-Quadruplex Ligand for Cancer Photodynamic Therapy Invited International journal

Keiko Kawauchi, Ryoto Urano, Natsuki Kinoshita, Shin Kuwamoto, Takeru Torii, Yoshiki Hashimoto, Shinya Taniguchi, Mitsuki Tsuruta, Daisuke Miyoshi

Genes 11 ( 11 ) 1340 2020.11

Joint Work

G-quadruplex (G4) is the non-canonical secondary structure of DNA and RNA formed by guanine-rich sequences. G4-forming sequences are abundantly located in telomeric regions and in the promoter and untranslated regions (UTR) of cancer-related genes, such as RAS and MYC. Extensive research has suggested that G4 is a potential molecular target for cancer therapy. Here, we reviewed G4 ligands as photosensitizers for cancer photodynamic therapy (PDT), which is a minimally invasive therapeutic approach. The photosensitizers, such as porphyrins, were found to be highly toxic against cancer cells via the generation of reactive oxidative species (ROS) upon photo-irradiation. Several porphyrin derivatives and analogs, such as phthalocyanines, which can generate ROS upon photo-irradiation, have been reported to act as G4 ligands. Therefore, they have been implicated as promising photosensitizers that can selectively break down cancer-related DNA and RNA forming G4. In this review, we majorly focused on the potential application of G4 ligands as photosensitizers, which would provide a novel strategy for PDT, especially molecularly targeted PDT (mtPDT).

DOI： 10.3390/genes11111340

PubMed

Precisely Controlled Reproducible Synthesis of Palladium Nanoparticles inside Metal-Organic Frameworks with H2 Gas as Reductant: Effects of Framework Crystallinity and H2 Gas Pressure Reviewed

Yohei Takashima, Yasushi Sato, Neo Kubo, Takaaki Tsuruoka, Kensuke Akamatsu

Chem. Lett. 50 ( 2 ) 244 - 247 2020.11

Authorship：Lead author,　Corresponding author

Artificial turn-on riboswitch to control target gene expression using a wild-type riboswitch splicing mechanism

TakahiroYamauchi, Takafumi Kubodera, Daisuke Miyoshi, Naoki Sugimoto, Shuji Hirohata

Journal of Bioscience and Bioengineering 2020.10

Joint Work

Mechanism toward Turn-on of Polysaccharide-Porphyrin Complexes for Fluorescence Probes and Photosensitizers in Photodynamic Therapy in Living Cells Reviewed International journal

Shodai Hino, Rikushi Funada, Kouta Sugikawa, Riku Kawasaki, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Atsushi Ikeda

CHEMMEDCHEM 15 ( 1 ) 1 - 12 2020.10

Joint Work

Publisher：WILEY-V C H VERLAG GMBH

beta-(1,3-1,6)-D-Glucan, lambda-carrageenan, tamarind gum, and pullulan can dissolve various porphyrin derivatives via the formation of complexes in water using a high-speed vibration milling method. The aqueous solutions of the resulting complexes exhibit long-term stability. Despite the adverse effects of the self-quenching process, notable fluorescence and improved photodynamic activity of the polysaccharide-complexed porphyrin derivatives were observed in the presence of liposomes, micelles, cyclodextrins, and HeLa cells. It was noted that the type of porphyrins was more important than the type of polysaccharides present in the complex. Porphyrin self-aggregates were monodispersed in the lipid membranes of the liposomes and lysosomes. The polysaccharide-complexed porphyrin derivatives showed increased photodynamic activity toward HeLa cells under photoirradiation between 610 and 740 nm.

DOI： 10.1002/cmdc.202000656

PubMed

Novel Strategy of Photodynamic Therapy Targeting RAS mRNA with G-Quadruplex Ligands for Cancer Treatment Invited Reviewed International journal

Takeru Torii, Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi

Journal of Data Mining in Genomics & Proteomics 11 ( 2 ) 2020.8

Joint Work

Authorship：Corresponding author

Dermal fibroblasts internalize phosphatidylserine-exposed secretory melanosome clusters and apoptotic melanocytes. Reviewed

21 ( 16 ) 5789 2020.8

Joint Work

DOI： 10.3390/ijms21165789

Modification of the Release Rate of Curcumin from the Cavity of Renatured beta-1,3-1,6-Glucan Nanoparticles by Changing the Molecular Weight of the Constituent Polysaccharide Chains Reviewed

Yuki Nonoguchi, Ayumu Kodama, Rika Unesaki, Nanako Doi, Toshio Suzuki, Kazuya Koumoto

CHEMISTRY LETTERS 49 ( 10 ) 1150 - 1153 2020.7

Joint Work

Publisher：CHEMICAL SOC JAPAN

We prepared renatured beta-1,3-1,6-glucan nanoparticles from molecular-weight-fractionated beta-1,3-1,6-glucans to analyze the molecular effects on the inclusion and release of the guest molecule curcumin. Glucan molecular weight strongly affects the amount of curcumin included in the complexes and its release rate. We found that the slow release rate of the incorporated curcumin is attributed not only to the stabilized stacking structure but also the slow migration between the internal and surface cavities characteristic of polymeric materials.

DOI： 10.1246/cl.200366

Photodynamic therapy targeting RAS mRNA

Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi

Medical Science Digest 46 ( 9 ) 26 - 27 2020.7

Joint Work

J-GLOBAL

Competing Roles of Two Kinds of Ligand during Nonclassical Crystallization of Pillared-Layer Metal-Organic Frameworks Elucidated Using Microfluidic Systems

Yoko Tanaka, Yu Kitamura, Ryuji Kawano, Kan Shoji, Moe Hiratani, Tetsuo Honma, Hikaru Takaya, Hirofumi Yoshikawa, Takaaki Tsuruoka, Daisuke Tanaka

Chemistry - A European Journal 26 ( 41 ) 8889 - 8896 2020.7

© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim To diversify metal-organic frameworks (MOFs), multi-component MOFs constructed from more than two kinds of bridging ligand have been actively investigated due to the high degree of design freedom afforded by the combination of multiple ligands. Predicting the synthesis conditions for such MOFs requires an understanding of the crystallization mechanism, which has so far remained elusive. In this context, microflow systems are efficient tools for capturing non-equilibrium states as they facilitate precise and efficient mixing with reaction times that correspond to the distance from the mixing point, thus enabling reliable control of non-equilibrium crystallization processes. Herein, we prepared coordination polymers with pillared-layer structures and observed the intermediates in the syntheses with an in-situ measurement system that combines microflow reaction with UV/Vis and X-ray absorption fine-structure spectroscopies, thereby enabling their rapid nucleation to be monitored. Based on the results, a three-step nonclassical nucleation mechanism involving two kinds of intermediate is proposed.

DOI： 10.1002/chem.202001438

PubMed

Novel Strategy of Photodynamic Therapy Targeting RAS mRNA with G-Quadruplex Ligands for Cancer Treatment Invited Reviewed

Takeru Torii, Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi

Journal of Data Mining in Genomics & Proteomics 2020.6

Joint Work

DOI： 10.35248/2153-0602.20.11.226.

An improved synthesis of 2′-O,4′-C-ethylene nucleic acid (ENA) and thermodynamic studies of duplex formation containing the guanosine ENA unit Reviewed

Miho Takagi-Sato, Koji Morita, Yoshiyuki Onishi, Yuuka Watahiki, Taku Ishigaki, Tomoka Akita, Erisa Tomita, Junji Kawakami, Makoto Koizumi

Nucleosides, Nucleotides & Nucleic Acids 39 ( 6 ) 838 - 852 2020.6

Publisher：Informa UK Limited

DOI： 10.1080/15257770.2019.1708389

Comparison of macrophage activation using γ-globulin and serum-derived macrophage activating factor. Reviewed

Akamatsu M, Mashiba R, Kawakatsu K, Ishikawa M, Nishikata T.

Anticancer Research 40 ( 8 ) 4707 - 4710 2020.6

Joint Work

DOI： 10.21873/anticanres.14471

RNA Phase Separation-Mediated Direction of Molecular Trafficking Under Conditions of Molecular Crowding Invited Reviewed

Kazuki Kohata, Daisuke Miyoshi

Biophys. Rev. 12 669 - 676 2020.5

Joint Work

Living cells are highly crowded with large and small biomolecules. The total concentration of biomolecules can reach 400 mg/ml, and 40% of the cell volume is occupied by biomolecules. Droplet formation in cells via liquid-liquid phase separation may play a role in controlling biochemical reactions in this complex molecular environment. Liquid-liquid phase separation generally involves nucleic acids and proteins as anionic and cationic components, respectively. Significant characteristics of droplets, which make them different from protein aggregation or fibril formation, are reversibility of formation and responsiveness to the molecular environment. In this review, we quantitatively describe the molecular environment inside cells and droplets that participate in controlling central dogma reactions. Finally, we discuss the importance of droplets under conditions of molecular crowding within living cells.

DOI： 10.1007/s12551-020-00696-3

Development of a chromophore-solid phase peptide reaction assay (C-SPRA) for assessing skin sensitization: In vitro Reviewed

Miyazaki, H., Hamada, Y., Takaishi, H., Minamino, Y., Ikeda, H., Mekata, H., Takaishi, M., Yamashita, K., Usui, K.

Analyst 145 3211 - 3216 2020.5

Joint Work

Authorship：Last author,　Corresponding author

DOI： 10.1039/c9an02514f

PubMed

Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA Reviewed

H. Tateishi-Karimata, D. Banerjee, T. Ohyama, S. Matsumoto, D. Miyoshi, S. Nakano, and N. Sugimoto

Biochemical and Biophysical Research Communications 525 ( 1 ) 177 - 183 2020.4

Joint Work

DOI： https://doi.org/10.1016/j.bbrc.2020.02.045

Coordination-Driven Self-Assembly on Polymer Surfaces for Efficient Synthesis of [Au(SPh)]<inf>n</inf> Coordination Polymer-Based Films

Takaaki Tsuruoka, Takashi Ohhashi, Jumpei Watanabe, Rikuto Yamada, Shoya Hirao, Yohei Takashima, Aude Demessence, Shefali Vaidya, Oleksandra Veselska, Alexandra Fateeva, Kensuke Akamatsu

Crystal Growth and Design 20 ( 3 ) 1961 - 1968 2020.3

Joint Work

Publisher：American Chemical Society ({ACS})

We describe a simple bottom-up strategy to generate [Au(SPh)] (SPh: thiophenolate) coordination polymer (CP) based thin films by employing an interfacial self-assembly on a nylon substrate. This method utilizes Au ion-doped nylon film as a precursor of [Au(SPh)] CP and a support substrate to control the kinetics of the reduction of the Au ion from Au(III) to Au(I) and the complexation of Au(I) ions as well as SPh on the surface of the nylon film. The obtained CPs exhibit a fibril morphology with high crystallinity, which leads to a luminescent property. Further, we systematically study the formation mechanism of [Au(SPh)] CPs on the substrates. Amorphous CPs formed at an early stage of reaction are transformed to crystalline CPs based on the rearrangement of components during the reaction. The findings described herein offer novel and interesting openings for the synthesis of Au ion-based CPs that demonstrate desired functions for a wide range of applications. n n n

DOI： 10.1021/acs.cgd.9b01622

Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA. Reviewed International journal

Hisae Tateishi-Karimata, Dipanwita Banerjee, Tatsuya Ohyama, Saki Matsumoto, Daisuke Miyoshi, Shu-Ich Nakano, Naoki Sugimoto

Biochemical and biophysical research communications 2020.2

Telomeric G-quadruplex topology has the ability to regulate telomerase activity, which counteracts the shortening of telomere with successive cell divisions, thereby causing genomic longevity. However, the detailed mechanism of G-quadruplexes topologies formed by telomeric sequences requires further investigation. In this study, we quantitatively investigated the effect of cosolutes, particularly the varying number of hydroxyl groups, on the structural transition between hybrid type and parallel G-quadruplexes formed by telomeric DNA sequences. Cosolutes with one or no hydroxyl groups in the vicinal position more efficiently induced the transition to parallel G-quadruplex from hybrid G-quadruplex than those with more hydroxyl groups. We also examined the effect of cosolute structures on the hydration of G-quadruplex formation; the results indicated that cosolutes with fewer hydroxyl groups lead to the release of greater amount of water during G-quadruplex formation. Molecular dynamics results showed that the parallel G-quadruplex was more dehydrated than the hybrid type G-quadruplex. Generally, a dehydrated structure is favored under crowding condition. Thus, depending on the surrounding cosolutes, the G-quadruplex topology can be controlled by the G-quadruplex hydration state.

DOI： 10.1016/j.bbrc.2020.02.045

PubMed

Solubilization of poorly water-soluble bioactive molecules in neutral aqueous media by complexation with renatured beta-1,3-1,6-glucan nanoparticles Reviewed International journal

Ayumu Kodama, Akifumi Nakagawa, Yuki Nonoguchi, Haruka Sakurai, Chieko Yano, Toshio Suzuki, Kazuya Koumoto

BIOPOLYMERS 111 ( 4 ) e23349 2020.2

Joint Work

Publisher：WILEY

The design of scaffolds for solubilizing/dispersing poorly water-soluble bioactive molecules in neutral aqueous media is a major challenge of functional food, pharmaceuticals, and cosmetics development, as highlighted by the plethora of corresponding solubilization/dispersion strategies. Herein, renatured beta-1,3-1,6-glucan (r-glucan) nanoparticles prepared by neutralization of alkali-denatured beta-1,3-1,6-glucan and subsequent centrifugation are used as a host to disperse water-insoluble bioactive molecules (curcumin, all-trans-retinoic acid, and rebamipide) by simple mixing of host and guest solutions. Curcumin in the r-glucan cavity is found to be stacked in the form of J-aggregates and twisted along the helix, and is demonstrated to be retained for significantly longer than curcumin in the corresponding gamma-cyclodextrin (gamma-CD) complex. Specifically, curcumin incorporated in gamma-CD is released within 5.5 hours, whereas that in the r-glucan complex is released very slowly, with 12% of curcumin in the latter complex retained after 31-day incubation at 37 degrees C. Thus, inclusion protocol simplicity and slow release ability make r-glucan nanoparticles a potential carrier scaffold for various applications.

DOI： 10.1002/bip.23349

PubMed

MMP24 as a Target of YAP is a Potential Prognostic Factor in Cancer Patients Reviewed International journal

Wataru Sugimoto, Katsuhiko Ito, Hiroaki Hirata, Yoshinori Abe, Takeru Torii, Yasumasa Mitsui, Yemima Budirahardja, Nobuyuki Tanaka, Keiko Kawauchi

Bioengineering 7 ( 18 ) 1 - 12 2020.2

Joint Work

Authorship：Last author,　Corresponding author

Therapeutic effect of intravenously administered autologous adipose-derived stem cells on chronic stage stroke patients. Reviewed

7 070 2020

Joint Work

DOI： 10.23937/2469-570X/1410070

Coordination-Driven Self-Assembly on Polymer Surface for Efficient Synthesis of [Au(SPh)]n Coordination Polymer-Based Films

T. Tsuruoka, T. Ohhashi, J. Watanabe, R. Yamda, S. Hirao, T. Takashima, A. Demessence, S. Vaidya, O. Veselska, A. Fateeva, K. Akamatsu

Cryst. Growth 1961 - 1968 2020

Single Work

Controlled syntheses of Ag nanoparticles inside MOFs by using amine-boranes as vapour phase reductants Reviewed

Yohei Takashima, Yasushi Sato, Takaaki Tsuruoka, Kensuke Akamatsu

Dalton Transactions 2020

Joint Work

Efficacy of Fermented Extract Obtained from Multiple-Processed Fermentation Technology of Thermophilic Bacteria and Yeast Reviewed

Daichi Kawano, Zhengzheng Liao, Jing Nie, Akira Date, Eduardo Perez, Jose Fernandez, Corey Webb, Kristen Huber, Jeffry B. Stock, Junji Kawakami, Zihua Fu

JOURNAL OF JAPANESE COSMETIC SCIENCE SOCIETY 44 ( 3 ) 194 - 202 2020

DOI： 10.11469/koshohin.44.194

Different reactivity of Sp and Rp isomers of phosphorothioate-modified oligonucleotides in a duplex structure Reviewed

Md Ariful Islam, Aki Fujisaka, Junji Kawakami, Takao Yamaguchi, Satoshi Obika

Bioorg. Med. Chem. Lett. 30 ( 14 ) 127166 - 127166 2020

Joint Work

Publisher：Elsevier BV

DOI： 10.1016/j.bmcl.2020.127166

Novel purification process for amyloid beta peptide(1-40) Invited Reviewed

Usui, K., Yokota, S.-I., Iwata, K., Hamada, Y.

Processes 8 2020

Joint Work

Authorship：Lead author,　Last author,　Corresponding author

DOI： 10.3390/PR8040464

Mass spectrometry-based solid phase peptide reaction assay for detecting allergenicity using an immobilized peptide-conjugating photo-cleavable linker Reviewed

Miyazaki, H., Samejima, Y., Iwata, K., Minamino, Y., Hikida, S., Ariumi, H., Ikeda, H., Hamada, Y., Yamashita, K., Usui, K.

International Journal of Molecular Sciences 21 2020

Joint Work

Authorship：Last author,　Corresponding author

DOI： 10.3390/ijms21218332

Synthesis of peptide-immobilized magnetic beads, and peptide reactivity assay for assessing skin sensitization utilizing chromophore Reviewed

Miyazaki, H., Takaishi, H., Ikeda, H., Ariumi, H., Hamada, Y., Yamashita, K., Usui, K.

Processes 8 2020

Joint Work

Authorship：Last author,　Corresponding author

DOI： 10.3390/pr8101257

Effect of tryptophan residues on gold mineralization by a gold reducing peptide Reviewed

Ozaki, M., Yoshida, S., Oura, M., Tsuruoka, T., Usui, K.

RSC Advances 10 2020

Joint Work

Authorship：Last author,　Corresponding author

DOI： 10.1039/d0ra07098j

Bioinspired cell nuclear nanotransporters generated by self‐assembly of amphiphilic polysaccharide‐amino acid derivatives conjugates Reviewed

Koji Nagahama, Yoshinori Sano, Mitsuo Inui, Seika Aoyama, Tokitaka Katayama, Kimika Ono

Advanced Biosystems 4 1900189 2020

Joint Work

美容医療　new horizon　－幹細胞への期待－ Invited Reviewed

市橋正光、長濱宏治

日本香粧品学会誌 44 30 - 35 2020

Joint Work

細胞移植用インジェクタブルゲルの開発 Invited Reviewed

長濱宏治

Precision Medicine 3 724 - 728 2020

Single Work

A thin hydrogel barrier linked onto cell surface sialic acids through covalent bonds induces cancer cell death in vivo Reviewed

Kimika Ono, Yuka Sanada, Yuka Kimura, Seika Aoyama, Natsumi Ueda, Tokitaka Katayama, Koji Nagahama

Biomaterials Science 8 577 - 585 2020

Joint Work

Controlled syntheses of Ag nanoparticles inside MOFs by using amine–borans as vapour phase reductants

Yohei Takashima, Yasushi Sato, Takaaki Tsuruoka, Kensuke Akamatsu

Dalton Transactions 49 ( 47 ) 17169 - 17172 2020

Joint Work

Publisher：Royal Society of Chemistry ({RSC})

In this work, by using a series of amine–boranes as vapour phase reductants, we succeeded in synthesizing Ag nanoparticles in a controlled manner inside MOFs.

DOI： 10.1039/D0DT03259J

Effect of tryptophan residues on gold mineralization by a gold reducing peptide

Makoto Ozaki, Shuhei Yoshida, Maho Oura, Takaaki Tsuruoka, Kenji Usui

RSC Advances 2020

Joint Work

DOI： 10.1039/D0RA07098J

Flexible and luminescent fibers of a 1D Au(i)–thiophenolate coordination polymer and formation of gold nanoparticle-based composite materials for SERS

Shefali Vaidya, Oleksandra Veselska, Antonii Zhadan, Marlène Daniel, Gilles Ledoux, Alexandra Fateeva, Takaaki Tsuruoka, Aude Demessence

Journal of Materials Chemistry C 2020

Joint Work

Publisher：Royal Society of Chemistry ({RSC})

DOI： 10.1039/D0TC01706J

Controlled syntheses of Ag nanoparticles inside MOFs by using amine-boranes as vapour phase reductants Reviewed

Y. Takashima, Y. Sato, T. Tsuruoka, K. Akamatsu

Dalton Trans 49 ( 47 ) 17169 - 17172 2020

Joint Work

Authorship：Lead author,　Corresponding author Publisher：Royal Society of Chemistry (RSC)

In this work, by using a series of amine–boranes as vapour phase reductants, we succeeded in synthesizing Ag nanoparticles in a controlled manner inside MOFs.

DOI： 10.1039/d0dt03259j

Improved Stability and Photodynamic Activity of Water-Soluble 5,15-Diazaporphyrins Incorporated in beta-(1,3-1,6)-d-Glucan with On-Off Switch Reviewed International journal

Shodai Hino, Shuhei Satake, Hideyuki Shinmori, Shigeki Kawabata, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Kouta Sugikawa, Riku Kawasaki, Atsushi Ikeda

CHEMISTRY-AN ASIAN JOURNAL 15 ( 3 ) 365 - 370 2019.12

Joint Work

Publisher：WILEY-V C H VERLAG GMBH

5,15-Diazaporphyrins, which have a large absorption at wavelengths over 600 nm, were dissolved in water by complex formation with beta-(1,3-1,6)-d-glucans. Aqueous solutions of these complexes were relatively stable compared with their trimethyl-beta-cyclodextrin-complexed analogues. beta-Glucan-complexed diazaporphyrins showed quenched fluorescence and had low singlet-oxygen-generation abilities owing to random self-aggregation. However, external stimuli, such as the presence of liposomes or intracellular uptake, restored the fluorescence and singlet-oxygen-generation abilities of beta-glucan-complexed diazaporphyrins. Consequently, beta-glucan-complexed diazaporphyrins showed very high photodynamic activities toward HeLa cells.

DOI： 10.1002/asia.201901582

PubMed

Selective recognition of human telomeric G-quadruplex with designed peptide via hydrogen bonding followed by base stacking interactions

Shikhar Tyagi, Sarika Saxena, Nikita Kundu, Taniya Sharma, Amlan Chakraborty, Sarvpreet Kaur, Daisuke Miyoshi, Jadala Shankaraswamy

RSC Advances 9 40255 - 40262 2019.12

Joint Work

We described a novel synthetic peptide in which a glutamine residue binds through hydrogen bonding to a guanine-base and a trytophan residue intercalates with K+ resulting in stabilization of a human telomeric G-quadruplex with high selectivity over its complementary c-rich strand and a double-stranded DNA and its complementary C-rich strand. This peptide offers great potential for cancer treatment by inhibiting the telomere extension by telomerase.

Structural effect of amine N-oxides on the facilitation of alpha-glucosidase-catalyzed hydrolysis reactions Reviewed International journal

Takuma Aoki, Yuichi Nakagawa, Ryutaro Genjima, Kazuya Koumoto

BIOPROCESS AND BIOSYSTEMS ENGINEERING 43 ( 3 ) 541 - 548 2019.11

Joint Work

Publisher：SPRINGER

Activation and stabilization of enzymes is an important issue in their industrial application. We recently reported that synthetic betaines, derived from cellular metabolites, structure-dependently increased the activity and stability of various enzymes including hydrolases, oxidases, and synthetases simply by mixing them into the reaction buffer. In this report, we focus on amine N-oxides, which are similarly important metabolites in cells with a highly polarized N-oxide bond, and investigate their enzyme stabilization and activation behavior. It was revealed that synthetic amine N-oxides structure-dependently activate alpha-glucosidase-catalyzed hydrolysis reactions similarly to betaines. The subsequent comparison of the kinetic parameters, the optimal concentration range for activation, and the maximal activity, suggested that amine N-oxides facilitate hydrolysis reactions via the same mechanism as betaines, because no differences were confirmed. However, the enzyme stabilization effect of amine N-oxides was slightly superior to that of betaines and the temporal stability of the enzyme in aqueous solutions was higher in the low amine N-oxide concentration range. The rheological properties, CD spectra, and dynamic fluorescence quenching experiments suggested that the suppression of unfavorable conformational perturbation was related to the difference in the hydration environments provided by the surrounding water molecules. Thus, we clarified that amine N-oxides facilitate enzyme reactions as a result of their similarity to betaines and provide a superior stabilizing effect for enzymes. Amine N-oxides show potential for application in enzyme storage and long-term reactions.

DOI： 10.1007/s00449-019-02248-w

PubMed

Turn-on fluorescence and photodynamic activity of beta-(1,3-1,6)-d-glucan-complexed porphyrin derivatives inside HeLa cells Reviewed International journal

Shodai Hino, Rikushi Funada, Kouta Sugikawa, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Atsushi Ikeda

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES 18 ( 12 ) 2854 - 2858 2019.11

Joint Work

Publisher：ROYAL SOC CHEMISTRY

Fluorescence intensities of water-soluble beta-(1,3-1,6)-d-glucan (beta-1,3-glucan)-complexed porphyrin derivatives were very weak as a result of self-quenching. However, beta-1,3-glucan-complexed tetra(aminophenyl)porphyrin exhibited 'off-state' to 'on-state' fluorescence switching activity by intracellular uptake. Furthermore, the internalised complex showed a high level of photodynamic activity toward HeLa cells under photoirradiation at long wavelengths.

DOI： 10.1039/C9PP00208A

PubMed

Bulky cations greatly increase the turnover of a native hammerhead ribozyme Reviewed

S. Nakano, H. Yamashita, K. Tanabe, N. Sugimoto

9 35820 - 35824 2019.11

Single Work

Authorship：Lead author,　Corresponding author

DOI： 10.1039/C9RA06797C

The RAS mRNA G-quadruplex: making undruggable cancer targets druggable

Keiko Kawauchi, Wataru Sugimoto, Daisuke Miyoshi

The Atlas of Science 2019.10

Joint Work

DMPK is a New Candidate Mediator of Tumor Suppressor p53-Dependent Cell Death. Reviewed

Itoh K, Ebata T, Hirata H, Torii T, Sugimoto W, Onodera K, Nakajima W, Uehara I, Okuzaki D, Yamauchi S, Budirahardja Y, Nishikata T, Tanaka N, Kawauchi K.

Molecules 24 ( 17 ) E3175 2019.9

Joint Work

DOI： 10.3390/molecules24173175

Mechanical regulation of bone homeostasis through p130Cas-mediated alleviation of NF-κB activity. Reviewed International journal

Miyazaki T, Zhao Z, Ichihara Y, Yoshino D, Imamura T, Sawada K, Hayano S, Kamioka H, Mori S, Hirata H, Araki K, Kawauchi K, Shigemoto K, Tanaka S, Bonewald FL, Honda H, Shinohara M, Nagao M, Ogata T, Harada I, Sawada Y.

Science Adv. 5 2019.9

Joint Work

Stabilization of DNA loop structures by large cations Reviewed

S. Nakano, T. Ayusawa, Y. Tanino, N. Sugimoto

J. Phys. Chem. B 123 7687 - 7694 2019.8

Joint Work

Authorship：Lead author,　Corresponding author

DOI： 10.1021/acs.jpcb.9b06074

研究課題設定を重視した科学技術人材育成プログラムROOTのデザインと実装

伊藤真之, 臼井英之, 長野寛之, 巳波弘佳, 甲元一也, 蛯名邦禎

日本科学教育学会年会論文集 43 399 - 400 2019.8

Joint Work

DOI： 10.14935/jssep.43.0_399

ケミカルバイオロジー2.0

三好大輔, 山東信介, 清中茂樹, 花岡健二郎, 後藤佑樹

現代化学 581 18 - 25 2019.7

Joint Work

Formation of Metal-Organic Frameworks on Metal Ion-Doped Polymer Substrate: In-Depth Time-Course Analysis using Scanning Electron Microscopy Reviewed

T. Tsuruoka, M. Hata, S. Hirao, T. Ohhashi, Y. Takashima, K. Akamatsu

Langmuir 35 10390 - 10396 2019.7

Joint Work

Control of the nucleation and growth processes of metal-organic frameworks using a metal ion-doped polymer substrate for the construction of continuous films Reviewed

T. Ohhashi, T. Tsuruoka, Y. Takashima, K. Akamatsu

CrystEngComm 21 4851 - 4854 2019.7

Joint Work

Characteristic morphological changes and rapid actin accumulation in serum-MAF-treated macrophages. Reviewed

Kawakatsu K, Ishikawa M, Mashiba R, Tran NK, Akamatsu M, Nishikata T

Anticancer Research 39 ( 8 ) 4533 - 4537 2019.6

Joint Work

DOI： 10.21873/anticanres.13630

A case of chronic actinic dermatitis that responded completely to treatment with oral colostrum-macrophage activating factor (colostrum-MAF). Reviewed

Ichihashi M, Nakamura Y, Muto M, Nishikata T, Inui T, Uto Y.

Photodermatol Photoimmunol Photomed 2019.5

Joint Work

DOI： 10.1111/phpp.12469

細胞の分子夾雑環境における核酸構造と核酸構造リガンドの挙動～細胞内でも望みの機能を発現する分子の合理設計に向けて～

三好大輔

生命化学研究レター 58 ( 1 ) 17 - 21 2019.4

Single Work

Enhancement of exon skipping activity by reduction in the secondary structure content of LNA-based splice-switching oligonucleotides Reviewed

T. Shimo, K. Tachibana, Y. Kawawaki, Y. Watahiki, T. Ishigaki, Y. Nakatsuji, T. Hara, J. Kawakami and S. Obika

Chem. Commun. 55 6850 - 6853 2019.4

Joint Work

Integrated Synthesis of Gold Nanoparticles Coated with Polyoxometalate Clusters Reviewed

Sergio Martin, Yohei Takashima, Chang-Gen Lin, Yu-Fei Song, Haralampos N. Miras, Leroy Cronin

Inorganic Chemistry 58 ( 7 ) 4110 - 4116 2019.3

Joint Work

Massive cytoplasmic transport and microtubule organization in fertilized chordate eggs. Reviewed

Nishikata T, Goto T, Yagi H, Ishii H.

Developmental Biology 448 ( 2 ) 154 - 160 2019.2

Joint Work

DOI： 10.1016/j.ydbio.2018.11.019

Non-centrosomal microtubule structures regulated by egg activation signaling contribute to cytoplasmic and cortical reorganization in the ascidian egg. Reviewed

Goto T, Kanda K, Nishikata T.

Developmental Biology 448 ( 2 ) 161 - 172 2019.2

Joint Work

DOI： 10.1016/j.ydbio.2018.10.014

Osmolyte-Enhanced Protein Synthesis Activity of a Reconstituted Translation System Reviewed

Yoshiki Moriizumi, Kazuhito V. Tabata, Daisuke Miyoshi, Hiroyuki Noji

ACS Synth Biol. 8 ( 3 ) 557 - 567 2019.2

Joint Work

DOI： 10.1021/acssynbio.8b00513

Mitochondrial protein E2F3d, a distinctive E2F3 product, mediates hypoxia-induced mitophagy in cancer cells. Reviewed International journal

Araki K, Kawauchi K, Sugimoto W, Tsuda D, Oda H, Yoshida R, Ohtani K.

Communications Biol. 6 550 - 561 2019.1

Joint Work

Formation of Metal-Organic Frameworks on Metal Ion-Doped Polymer Substrate: In-Depth Time-Course Analysis using Scanning Electron Microscopy Reviewed

T. Tsuruoka, M. Hata, S. Hirao, T. Ohhashi, T. Takashima, K. Akamatsu

Langmuir 35 10390 - 10396 2019

Joint Work

Control of the nucleation and growth processes of metal-organic frameworks using a metal ion-doped polymer substrate for the construction of continuous films Reviewed

T. Ohhashi, T. Tsuruoka, T. Takashima, K. Akamatsu

CrystEngComm 21 4851 - 4854 2019

Joint Work

固液界面での選択的MOF形成

鶴岡孝章、平尾翔也、髙嶋洋平、赤松謙祐

日本接着学会誌 55 301 - 307 2019

Joint Work

細胞移植用スマートゲルの開発 Invited

長濱宏治、小野公佳、木村友香、青山青海

ケミカルエンジニヤリング 64 17 - 26 2019

Joint Work

Formation of Metal-Organic Frameworks on Metal Ion-Doped Polymer Substrate: In-Depth Time-Course Analysis using Scanning Electron Microscopy Reviewed

T. Tsuruoka, M. Hata, S. Hirao, T. Ohhashi, T. Takashima, K. Akamatsu

Langmuir 35 10390 - 10396 2019

Joint Work

Control of the nucleation and growth processes of metal-organic frameworks using a metal ion-doped polymer substrate for the construction of continuous films Reviewed

T. Ohhashi, T. Tsuruoka, T. Takashima, K. Akamatsu

CrystEngComm 21 4851 - 4854 2019

Joint Work

固液界面での選択的MOF形成

鶴岡孝章、平尾翔也、髙嶋洋平、赤松謙祐

日本接着学会誌 55 301 - 307 2019

Joint Work

Metal sensitive and DNA concentration dependent structural rearrangement of short oligonucleotide into large suprastructures Reviewed

J. Shankaraswamy, Shikhar Tyagi, Anju Singh, Daisuke Miyoshi, Sarika Saxena

Journal of Biomolecular Structure and Dynamics 37 ( 9 ) 2211 - 2218 2018.12

Joint Work

DOI： 10.1080/07391102.2018.1484816

Wrapping Flexible Metal-Organic Framework with Organic Polymers via Site-Specific Radical Polymerization from Its Crystal Surface Invited Reviewed

Yohei Takashima, Kenji Fukudome, Atsuto Horikoshi, Takaaki Tsuruoka, Kensuke Akamatsu

Polyhedron 155 275 - 280 2018.11

Joint Work

Authorship：Lead author

Wrapping flexible metal–organic framework with organic polymers via site-specific radical polymerization from its crystal surface

Yohei Takashima, Kenji Fukudome, Atsuto Horikoshi, Takaaki Tsuruoka, Kensuke Akamatsu

Polyhedron 155 275 - 280 2018.11

Joint Work

© 2018 Elsevier Ltd In this study, we succeeded in the crystal surface modification of metal–organic framework (MOF) with an organic polymer by site-specific radical polymerization. A MOF was selected with interpenetrated structure containing a naphthalenediimide moiety with electron accepting capability. N, N-dimethylaniline, as an electron donating guest, induced electron transfer to the napthalenediimide moiety, generating radical anions on the pore and crystal surfaces, initiating radical polymerization. The resulting composite was examined in detail with XRD, TG, TEM, ICP, IR, gas adsorption, and fluorescent measurements, demonstrating that an organic polymer was generated only on the crystal surface, preserving the original porous property. In addition, higher structural stability in response to H2O was observed after polymer wrapping.

DOI： 10.1016/j.poly.2018.08.057

Anti-inflammatory effect of 5-aminolevulinic acid on macrophage in vitro. Reviewed

Ishikawa M, Mashiba R, Kawakatsu K, Nakagawa H, Kitajima Y, Ishizuka M, Nishikata T.

ALA-Porphyrin Science 7 ( 1 ) 3 - 11 2018.9

Joint Work

Gold-Titania Nanocatalyst Generated by Mineralization Using Two Artificial Peptides with DNA Reviewed

Ozaki Makoto, Tomizaki Kin-Ya, Hamada Yoshio, Usui Kenji

JOURNAL OF PEPTIDE SCIENCE 24 S101 2018.9

Effects of platinum and palladium nanocolloid on macrophage polarization in relevance to repigmentation of vitiligo. Reviewed

Minamiyama Y, Ishikawa M, Shibata T, Tsuji G, Nishikata T, Takemura S, Ichihashi M.

J Cutan Immunol Allergy 1 139 - 146 2018.8

Joint Work

巻頭言変化は突然に ―学会参加のススメ―

三好大輔

日本化学会生体機能関連化学部会ニュースレター 33 ( 2 ) 2 - 3 2018.8

Single Work

Phagocytic activation of macrophages with serum MAF depends on engulfment efficiency and not migratory activity. Reviewed

Mashiba R, Ishikawa M, Sumiya YU, Kawakatsu K, Tran NK, Nishikata T.

Anticancer Res. 38 ( 7 ) 4295 - 4298 2018.7

Joint Work

DOI： 10.21873/anticanres.12727

An anionic phthalocyanine decreases NRAS expression by breaking down its RNA G-quadruplex Reviewed

Keiko Kawauchi, Wataru Sugimoto, Takatoshi Yasui, Kohei Murata, Katsuhiko Itoh, Kazuki Takagi, Takaaki Tsuruoka, Kensuke Akamatsu, Hisae Tateishi-Karimata, Naoki Sugimoto, and Daisuke Miyoshi

Nature Communicationsvolume 9 2271 2018.6

Joint Work

Authorship：Lead author

DOI： 10.1038/s41467-018-04771-y

An anionic phthalocyanine decreases NRAS expression by breaking down its RNA G-quadruplex Reviewed International journal

Kawauchi K, Sugimoto W, Yasui T, Murata K, Itoh K, Takagi K, Tsuruoka T, Akamatsu K, Tateishi-Karimata H, Sugimoto N, Miyoshi D.

Nature Commun. 9 2271 2018.6

Joint Work

Authorship：Lead author,　Corresponding author

Living functional hydrogels generated by bioorthogonal cross-linking reactions of azide-modified cells with alkyne-modified polymers Reviewed

K. Nagahama, Y. Kimura, A. Takemoto

Nature Communications ( 9 ) 2195 2018.6

Joint Work

Authorship：Lead author

Thermal stability and conformation of DNA and proteins under the confined condition in the matrix of hydrogels

S. Nakano, D. Yamaguchi, and N. Sugimoto

Mol. Biol. Rep. ４5 403 - 411 2018.4

Joint Work

Authorship：Lead author

DOI： 10.1007/s11033-018-4174-z

Modification of the N-terminus of a calcium carbonate precipitating peptide affects calcium carbonate mineralization Reviewed

Kenji Usui, Shin-Ichiro Yokota, Makoto Ozaki, Shungo Sakashita, Takahito Imai, Kin-Ya Tomizaki

Protein and Peptide Letters 25 42 - 47 2018.4

Joint Work

Authorship：Lead author Publisher：Bentham Science Publishers B.V.

Background: A core sequence (the 9 C-terminal residues) of calcification-associated peptide (CAP-1) isolated from the exoskeleton of the red swamp crayfish was previously shown to control calcium carbonate precipitation with chitin. In addition, a modified core sequence in which the phosphorylated serine at the N terminus is replaced with serine exhibits was also previously shown to alter precipitation characteristics with chitin. Objectives: We focused on calcium carbonate precipitation and attempted to elucidate aspects of the mechanism underlying mineralization. We attempted to evaluate in detail the effects of modifying the N-terminus in the core sequence on calcium carbonate mineralization without chitin. Methods: The peptide modifications included phosphorylation, dephosphorylation, and a free or acetylated Nterminus. The peptides were synthesized manually on Wang resin using the DIPCI-DMAP method for the first residue, and Fmoc solid phase peptide synthesis with HBTU-HOBt for the subsequent residues. Prior to calcium carbonate precipitation, calcium carbonate was suspended in MilliQ water. Carbon dioxide gas was bubbled into the stirred suspension, then the remaining solid CaCO3 was removed by filtration. The concentration of calcium ions in the solution was determined by standard titration with ethylenediaminetetraacetate. Calcium carbonate precipitation was conducted in a micro tube for 3 h at 37°C. We used the micro-scale techniques AFM (atomic force microscopy) and TEM (transmission electron microscopy), and the macro-scale techniques chelate titration, HPLC, gel filtration, CD (circular dichroism) and DLS (dynamic light scattering). Results: We determined the morphologies of the calcium carbonate deposits using AFM and TEM. The pS peptide provided the best control of the shape and size of the calcium carbonate round particles. The acetylated peptides (Ac-S and Ac-pS) provided bigger particles with various shapes. S peptide provided a mixture of bigger particles and amorphous particles. We verified these findings using DLS. All the peptide samples produced nanostructures of the expected size in agreement with the AFM and TEM results. We estimated the abilities of these peptides to precipitate calcium carbonate by determining the residual calcium hydrogen carbonate concentration by standard titration with ethylenediaminetetraacetate after calcium carbonate precipitation. The Ac-pS peptide showed the lowest residual calcium hydrogen carbonate concentration whereas the S peptide showed the highest, suggesting that the precipitating activities of these peptides towards calcium carbonate correlated with peptide net charge. Then the gel filtration results showed a large oligomer peak and a small oligomer/monomer peak for all peptide samples in agreement with the AFM, TEM and DLS results. CD measurements showed that all the peptides formed random-coil-like structures. Thus, we used both macro-and micro-observation techniques such as chelate titration, DLS, AFM and TEM to show that the calcium carbonate precipitating activities of four derivatives of the core sequence of CAP-1 may correlate with the peptide net charge. Conclusion: These peptides mainly act as a catalyst rather than as a binder or component of the calcium carbonate deposits (as a template). On the other hand, the morphologies of the calcium carbonate deposits appeared to be dependent on the ability of the peptide to assemble and act as a template. Consequently, elucidating the relationship between peptide sequence and the ability of the peptide to assemble would be indispensable for controlling precipitate morphologies in the near future. This knowledge would provide important clues for elucidating the relationship between peptide sequence and mineralization ability, including deposit morphology and precipitating activity, for use in nanobiochemistry and materials chemistry research.

DOI： 10.2174/0929866525666171221114658

Applications of a novel biodetection system to saliva using protein fingerprints with data processing Reviewed

Yuki Tominaga, Kenji Usui, Akiyoshi Hirata, Hiro-O Ito, Kiyoshi Nokihara

Bioorganic and Medicinal Chemistry 26 3210 - 3216 2018.4

Joint Work

Authorship：Lead author Publisher：Elsevier Ltd

A fundamental method has been developed focusing on a facile and rapid examination of periodontal disease. Periodontal disease is an oral disease thought to affect 80% of adults, and early detection with treatment is desirable for the improvement of the quality of life. Unfortunately conventional methods are not consistent as the disease is caused by a number of undefined bacteria and detection relies on the skills of the dentist. Thus an objective detection system is required. We have performed an experiment on saliva using a novel biodetection system, designated PepTenChip®. A disease model for saliva was prepared using a specimen from a healthy subject and a mixture of hemoglobin (f-Hb) and lactate dehydrogenase (LDH), which is used as a periodontal disease marker protein with healthy saliva. PepTenChip® is a peptide microarray in which fluorescent labelled structured peptides are immobilized on a novel amorphous carbon substrate. Since the peptides used as capture molecules are fluorescently labelled, labeling of analytes is not necessary. The fluorescence intensity change before and after application of analytes are detected rather than the ON/OFF detection common to conventional microarrays using a set of antigen–antibody. The fluorescence intensity value changes according to the concentration of captured protein allowing the generation of protein fingerprint (PFP) and dendrograms. The present method does not rely on a “one to one” interaction, unlike conventional biodetection, and advantages can be envisaged in the case of an undefined or unknown cause of disease. The statistical analyses, such as multivariate analyses, allow classification of the type of proteins added in saliva as mimetics of disease. PepTenChip® system is useful and convenient for examination of periodontal disease in health care.

DOI： 10.1016/j.bmc.2018.04.049

Peptides for Silica Precipitation: Amino Acid Sequences for Directing Mineralization Reviewed

Makoto Ozaki, Shungo Sakashita, Yoshio Hamada, Kenji Usui

Protein & Peptide Letters 25 15 - 24 2018.4

Joint Work

Publisher：Bentham Science Publishers B.V.

Background: Peptides are promising compounds for use in inorganic or organic-inorganic hybrid syntheses (mineralization) and offer several advantages over proteins. Meanwhile, silica-based nanomaterials have been extensively investigated for many years because of their potential application in a diverse range of technologies, including catalysis, sensing, separation, enzyme immobilization, and gene and drug delivery. Considerable progress has been made over the past decade in understanding the molecular mechanisms underpinning biosilicification and the biomimetic synthesis of patterned nanosilica using peptides. Objectives: This mini-review focuses on various peptide sequences, especially short peptide sequences (30 residues or less), for silica mineralization. Methods: We first briefly review early studies on silica mineralization using proteins to provide background information. This is followed by a discussion of promising peptide sequences and attempts to discern the relationship between amino acid sequence, their potential for mineralization, and the properties of the mineral product. Results: The synthetic control of silica mineralization using engineered proteins, such as recombinant silicateins and silaffins, was inspired by silica biomineralization by natural proteins from organisms (sponges, diatoms, and plants). Concurrently, several papers described the utility of well-structured protein assemblies as templates for silica mineralization. These template-directed syntheses of well-structured silica deposits were first conducted using natural proteins or protein assemblies such as collagen fibers and virus hollow protein tubes. Then we reviewed a selection of short peptides (30 residues or less) that had been successfully used for silica mineralization. Almost all peptides developed to date can be sorted by classification like proteins (synthetic control of silica mineralization or utility of templates for silica mineralization): the first class of peptides is used for peptide-directed synthesis, and the second is used for template-directed synthesis after the peptides have assembled and formed nanostructure such as fibers and tubes. The presented peptides were classified and arranged according to the classification. Additionally, we briefly introduced silica mineralization triggered by the combination of short silica-precipitating peptides and template molecules. Conclusion: In this mini-review we focused on various peptide sequences, especially short peptide sequences of 30 residues or less, designed for silica mineralization. The peptides have been used both for peptide-directed silica mineralization and for template-directed silica mineralization. The recent advances in peptide-driven mineralization reviewed here suggest that it will soon be possible to completely control the silica mineralization process using peptides. Mineralization systems using peptides will provide researchers with new tools for controlling various inorganic syntheses and the production of organic-inorganic materials for nanobiochemistry and materials chemistry research.

DOI： 10.2174/0929866525666171214111007

Editorial: Organic-Inorganic Hybrid Materials and Their Applications Reviewed

Kin-ya Tomizaki, Yoshio Hamada, Kenji Usui

Protein & Peptide Letters 25 2 - 3 2018.4

Joint Work

Authorship：Lead author

DOI： 10.2174/092986652501180418150844

Substrate rigidity-dependent positive feedback regulation between YAP and ROCK2. Reviewed International journal

Sugimoto W, Itoh K, Mitsui Y, Ebata T, Fujita H, Hirata H, Kawauchi K

Cell adhesion & migration 12 ( 2 ) 101 - 108 2018.3

Joint Work

Authorship：Last author,　Corresponding author

DOI： 10.1080/19336918.2017.1338233

PubMed

Nanocomposite injectable gels capable of self-replenishing regenerative extracellular microenvironments for in vivo tissue engineering Reviewed

K. Nagahama, N. Oyama, K. Ono, A. Hotta, K. Kawauchi, T. Nishikata

Biomater Sci. 6 ( 3 ) 550 - 561 2018.2

Joint Work

DOI： 10.1039/c7bm01167a.

Nanocomposite injectable gels capable of self-replenishing regenerative extracellular microenvironments for in vivo tissue engineering Reviewed International journal

Koji Nagahama, Naho Oyama, Kimika Ono, Atsushi Hotta, Keiko Kawauchi, Takahito Nishikata

Biomater Sci. 6 550 - 561 2018.2

Joint Work

A high-throughput quantitative assay system for macrophage phagocytic activity. Reviewed

Mami Ishikawa, Riho Mashiba, Kumpei Kawakatsu, Ngoc Kiet Tran, Takahito Nishikata

macrophage 2018.2

Joint Work

Nanocomposite injectable gels capable of self-replenishing regenerative extracellular microenvironments for in vivo tissue engineering Reviewed

K. Nagahama, N. Oyama, K. Ono, A. Hotta, K. Kawauchi, T. Nishikata

Biomaterials Science ( 6 ) 550 - 561 2018.2

Joint Work

Authorship：Lead author

Destabilization of DNA G-quadruplexes by chemical environment changes during tumor progression facilitates transcription Reviewed International journal

Hisae Tateishi-Karimata, Keiko Kawauchi, Naoki Sugimoto

J Am Chem Soc. 140 642 - 651 2018.1

Joint Work

Crystal conversion between metal-organic frameworks with different crystal topologies for efficient crystal design on two-dimensional substrates Reviewed

T. Tsuruoka, K. Inoue, A. Miyanaga, K. Tobiishi, T. Ohhashi, M. Hata, Y. Takashima, K. Akamatsu

J. Cryst. Growth 487 1 - 7 2018

Joint Work

ポリイミドフィルムの表面改質と銅薄膜のダイレクトめっき技術

髙嶋洋平、鶴岡孝章、赤松謙祐

MATERIAL STAGE 18 29 - 33 2018

Joint Work

Wrapping Flexible Metal-Organic Framework with Organic Polymers via Site-Specific Radical Polymerization from Its Crystal Surface

Y. Takashima, K. Fukudome, A. Horikoshi, T. Tsuruoka, K. Akamats

Polyhedron 155 275 - 280 2018

Joint Work

Controlling the Orientation of Metal−Organic Framework Crystals by an Interfacial Growth Approach Using a Metal Ion-Doped Polymer Substrate

T. Ohhashi, T. Tsuruoka, S. Fujimoto, Y. Takashima, K. Akamatsu

Cryst. Growth Des 18 402 - 408 2018

Joint Work

Controlling the Orientation of Metal−Organic Framework Crystals by an Interfacial Growth Approach Using a Metal Ion-Doped Polymer Substrate

T. Ohhashi, T. Tsuruoka, S. Fujimoto, Y. Takashima, K. Akamatsu

Cryst. Growth Des 18 402 - 408 2018

Joint Work

ポリイミドフィルムの表面改質と銅薄膜のダイレクトめっき技術

MATERIAL STAGE 18 29 - 33 2018

Single Work

Wrapping Flexible Metal-Organic Framework with Organic Polymers via Site-Specific Radical Polymerization from Its Crystal Surface

Y. Takashima, K. Fukudome, A. Horikoshi, T. Tsuruoka, K. Akamatsu

Polyhedron 155 275 - 280 2018

Joint Work

Design of anti-alzheimer’s disease agents focusing on a specific interaction with target biomolecules Reviewed

Yoshio Hamada, Kenji Usui

Neuromethods 132 207 - 228 2018

Joint Work

Publisher：Humana Press Inc.

Alzheimer’s disease (AD) is the most common cause of dementia, characterized by progressive intellectual deterioration. Amyloid β peptide (Aβ), the main component of senile plaques in the brains of patients with AD, is formed from amyloid precursor protein (APP) by two processing enzymes. According to the amyloid hypothesis, a processing enzyme β-secretase (BACE1
β-site APP cleaving enzyme) that triggers Aβ formation in the rate-limiting first step of Aβ processing appears to be a promising molecular target for therapeutic intervention in AD. Many researchers have revealed BACE1 inhibitors for the AD treatment. Early BACE1 inhibitors were designed based on the first reported X-ray crystal structure, 1FKN, of a complex between recombinant BACE1 and inhibitor OM99-2. Although OM99-2 seemed to interact with BACE1-Arg235 side chain by hydrogen bonding, we found that a quantum chemical interaction, such as σ-π interaction or π-π stacking, plays a critical role in BACE1 inhibition mechanism. Moreover, we proposed a novel “electron-donor bioisostere” concept in drug discovery study and designed potent BACE1 inhibitors using this concept.

DOI： 10.1007/978-1-4939-7404-7

Channel current analysis estimates the pore-formation and the penetration of transmembrane peptides Reviewed

Sekiya, Y., Sakashita, S., Shimizu, K., Usui, K., Kawano, R.

Analyst 143 ( 15 ) 2018

Joint Work

DOI： 10.1039/c8an00243f

Crystal conversion between metal-organic frameworks with different crystal topologies for efficient crystal design on two-dimensional substrates Reviewed

T. Tsuruoka, K. Inoue, A. Miyanaga, K. Tobiishi, T. Ohhashi, M. Hata, Y. Takashima, K. Akamatsu

J. Cryst. Growth 487 1 - 7 2018

Joint Work

Controlling the Orientation of Metal−Organic Framework Crystals by an Interfacial Growth Approach Using a Metal Ion-Doped Polymer Substrate Reviewed

T. Ohhashi, T. Tsuruoka, S. Fujimoto, Y. Takashima, K. Akamatsu

Cryst. Growth Des 18 402 - 408 2018

Joint Work

Wrapping Flexible Metal-Organic Framework with Organic Polymers via Site-Specific Radical Polymerization from Its Crystal Surface

Y. Takashima, K. Fukudome, A. Horikoshi, T. Tsuruoka, K. Akamatsu

Polyhedron 155 275 - 280 2018

Joint Work

ポリイミドフィルムの表面改質と銅薄膜のダイレクトめっき技術

髙嶋洋平、鶴岡孝章、赤松謙祐

MATERIAL STAGE 18 29 - 33 2018

Joint Work

Controlling the Orientation of Metal-Organic Framework Crystals by an Interfacial Growth Approach Using a Metal Ion-Doped Polymer Substrate Reviewed

Takashi Ohhashi, Takaaki Tsuruoka, Seiya Fujimoto, Yohei Takashima, Kensuke Akamatsu

Cryst. Growth Des. 18 402 - 408 2018

Joint Work

Crystal Conversion between Metal-Organic Frameworks with Different Crystal Topologies for Efficient Crystal Design on Two Dimensional Substrates Reviewed

Takaaki Tsuruoka, Kohei Inoue, Ayumi Miyanaga, Kaho Tobiishi, Takashi Ohhashi, Manami Hata, Yohei Takashima, Kensuke Akamatsu

J. Cryst. Growth 487 1 - 7 2018

Joint Work

Tuning Light Emission towards White Light from a Naphthalenediimide-Based Entangled Metal-Organic Framework by Mixing Aromatic Guest Molecules Invited Reviewed

Rebeca Sola-Llano, Virginia Martínez-Martínez, Shuhei Furukawa, Yohei Takashima, Iñigo López-Arbeloa

Polymers 10 188 2018

Joint Work

Modulation of the ribozyme and deoxyribozyme activities using tetraalkylammonium ions Reviewed

S. Nakano, T. Watabe, and N. Sugimoto

ChemPhysChem 18 ( 24 ) 3614 - 3619 2017.12

Joint Work

Authorship：Lead author

DOI： 10.1002/cphc.201700882

Other Link： https://www.ncbi.nlm.nih.gov/pubmed/28940937

生体分子の挙動解析研究を目標としたマイクロ波照射システムの開発　～ペプチドのバイオミネラリゼーションにおけるマイクロ波影響解析をモデルとして～ Reviewed

臼井健二, 富樫浩行, 圓東那津実, 尾崎誠, 有本米次郎, 裏鍛武史, 大沢隆二, 皆木幸一, 中西伸浩, 梅谷智弘

日本電磁波エネルギー応用学会論文誌 17 - 24 2017.12

Joint Work

Authorship：Lead author

Catalytic activities of ribozymes and DNAzymes in water and mixed aqueous media Invited Reviewed

S. Nakano, M. Horita, M. Kobayashi, and N. Sugimoto

Catalysis 7 ( 12 ) 355 - 348 2017.11

Joint Work

Authorship：Lead author

DOI： org/10.3390/catal7120355

Other Link： http://www.mdpi.com/2073-4344/7/12/355

DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity Reviewed

Kenji Usu, Arisa Okada, Shungo Sakashita, Masayuki Shimooka, Takaaki Tsuruoka, Shu-ichi Nakano, Daisuke Miyoshi, Tsukasa Mashima, Masato Katahira and Yoshio Hamada

Molecules 22 1991 2017.11

Joint Work

DOI： 10.3390/molecules22111991

Other Link： http://www.mdpi.com/1420-3049/22/11/1991

Influence of intracellular environment on allosteric ribozyme activity

Misaki Kameno, Mika Sawada, Nae Sakimoto and Junji Kawakami

Proc. 44th Intl. Symp. Nucleic Acid Chemistry 178 - 179 2017.11

Joint Work

Authorship：Lead author

Quantification of stabilization effect of co-solutes on RNA tertiary interaction

Natsumi Sasaki, Daisuke Miyoshi and Junji Kawakami

Proc. 44th Intl. Symp. Nucleic Acid Chemistry 174 - 175 2017.11

Joint Work

Authorship：Lead author

Non-Covalent Loading of Anti-Cancer Doxorubicin by Modularizable Peptide Self-Assemblies for a Nanoscale Drug Carrier Reviewed

Kin-ya Tomizaki, Kohei Kishioka, Shunsuke Kataoka, Makoto Miyatani, Takuya Ikeda, Mami Komada, Takahito Imai, Kenji Usui

MOLECULES 22 ( 11 ) 1916 2017.11

Joint Work

Authorship：Lead author Publisher：MDPI AG

We prepared nanoscale, modularizable, self-assembled peptide nanoarchitectures with diameters less of than 20 nm by combining beta-sheet-forming peptides tethering a cell-penetrating peptide or a nuclear localization signal sequence. We also found that doxorubicin (Dox), an anti-cancer drug, was non-covalently accommodated by the assemblies at a ratio of one Dox molecule per ten peptides. The Dox-loaded peptide assemblies facilitated cellular uptake and subsequent nuclear localization in HeLa cells, and induced cell death even at low Dox concentrations. This peptide nanocarrier motif is a promising platform for a biocompatible drug delivery system by altering the targeting head groups of the carrier peptides.

DOI： 10.3390/molecules22111916

細胞環境化学模倣実験系におけるDNAの構造とその熱力学的安定性

三好大輔

日本核酸化学会誌 1 13 - 19 2017.10

Single Work

Newly characterized interaction stabilizes DNA structure: oligoethylene glycols stabilize G-quadruplexes CH–π interactions Reviewed

H. Tateishi-Karimata, T. Ohyama, T. Muraoka, P. Podbevsek, A. M. Wawro, S. Tanaka, S. Nakano, K. Kinbara, J. Plavec, N. Sugimoto

Nucleic Acids Res. 45 7021 - 7030 2017.7

Joint Work

DOI： 10.1093/nar/gkx299

Other Link： https://www.ncbi.nlm.nih.gov/pubmed/28453855

Fluorescent and luminescent fusion proteins for analyses of amyloid beta peptide aggregation Reviewed

Kenji Usui, Masayasu Mie, Takashi Andou, Hisakazu Mihara, Eiry Kobatake

JOURNAL OF PEPTIDE SCIENCE 23 ( 7-8 ) 659 - 665 2017.7

Joint Work

Authorship：Lead author Publisher：WILEY

The amyloid beta (A) peptide is regarded as a causative agent of Alzheimer's disease. In this study, fluorescent and luminescent fusion proteins were constructed to analyze A aggregation. A system was developed to monitor changes in luminescence that provides information about A aggregation. In the presence of monomeric A, the fusion protein exhibits higher luminescence intensity, and the luminescence intensity is diminished after aggregation of the fusion protein and A. In contrast, the fluorescence is sustained in the presence of A. In the absence of A, the fusion protein self-aggregates, and its luminescence and fluorescence are quenched, thus decreasing the background fluorescence and enhancing the detection of A inside and outside the cells. The ratio of the luminescence intensity to the fluorescence intensity would allow the aggregation degrees of A to be distinguished. This study would be a promising method for analyzing the aggregation state of a particular amyloid protein/peptide (monomer, oligomer, or fibril), as well as the distribution of the amyloid protein/peptide within and at the cell surface, by using a single fusion protein. Copyright (c) 2017 European Peptide Society and John Wiley & Sons, Ltd.

DOI： 10.1002/psc.3003

Quantitative relationship between chemical properties and bioactivities of anti-microRNA oligonucleotides targeted to tumor-associated microRNA-21 Reviewed

Koji Nagahama, Kenta Iseda, Daichi Kawano, Junji Kawakami

BIOCHIMIE 137 124 - 131 2017.6

Joint Work

Authorship：Last author,　Corresponding author Publisher：ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER

Synthetic anti-microRNA oligonucleotides (AMOs) are promising drug candidates to inactivate disease related microRNAs because of their sequence-specific binding to their targets and the variety of chemical modifications available. Over the last decade, the qualitative relationships between the chemical properties of AMOs and bioactivity (inactivation of their target miRNAs) have been studied to enhance their bioactivity. On the other hand, in real-world drug development, drugs must be designed case-by case, taking many factors into account. Thus, in order to design AMOs that target specific miRNA, understanding the quantitative relationship between the chemical properties of AMOs and inactivation of their target miRNA is necessary. Here, we aimed to find the specific quantitative relationship of AMOs targeted to tumor-associated miR-21 through direct comparison of their inactivation efficacies with systematically varied chemical properties, including sequence-specific binding affinity, nuclease resistance, and RNase H activation. As a result, we newly found the quantitative relationships; (1) sequence specific binding affinity of AMOs against miR-21 is the main determining factor for inactivation efficacy, (2) nuclease resistance of AMOs impacts their miR-21 inactivation efficacy acting cooperatively with the binding affinity, although nuclease resistance alone does not affect the miRNA inactivation efficacy, and (3) RNase H activation is unnecessary. This study also demonstrates the utility of the obtained relationship for the design of AMO-based drugs targeted to miR-21, through cell-based analyses. Thus, the obtained quantitative relationship would make it possible to predict the miR-21 inactivation efficacy of AMOs which are newly designed. (C) 2017 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.

DOI： 10.1016/j.biochi.2017.03.003

Quantitative relationship between chemical properties and bioactivities of anti-microRNA oligonucleotides targeted to tumor-associated microRNA-21 Reviewed

Koji Nagahama, Kenta Iseda, Daichi Kawano, Junji Kawakami

Biochimie 137 124 - 131 2017.6

Joint Work

Authorship：Lead author

NF-kappaB prevents oncogenic Ras-induced b-actin cleavage in p53-deficient cells Reviewed

Wataru Sugimoto, Katsuhiko Itoh, Toshiya Kotari, Alvin K. Guo,Takahiro Ebata, Hiroaki Hirata, Keiko Kawauchi

International Journal of Cancer & Cellular Biology Research 2 ( 1 ) 15 - 18 2017.5

Joint Work

Authorship：Lead author

Other Link： http://scireslit.com/Cancer/IJCCR-ID15.pdf

Highly Sensitive Telomerase Assay Insusceptible to Telomerase and Polymerase Chain Reaction Inhibitors for Cervical Cancer Screening Using Scraped Cells. Reviewed

Hidenobu Yaku , Yoshio Yoshida, Hidehiko Okazawa, Yasushi Kiyono, Yuko Fujita, Daisuke Miyoshi

Anal. Chem. 89 ( 13 ) 6948 - 6953 2017.5

Joint Work

DOI： 10.1021/acs.analchem.6b04777

PEG-based nanocomposite hydrogel: Thermoresponsive sol-gel transition controlled by PLGA-PEG-PLGA molecular weight and solute concentration Reviewed

Makoto Miyazaki, Tomoki Maeda, Kenji Hirashima, Naruki Kurokawa, Koji Nagahama, Atsushi Hotta

Polymer 115 246 - 254 2017.4

Joint Work

Authorship：Lead author

Quantitative relationship between chemical properties and bioactivities of anti-microRNA oligonucleotides targeted to tumor-associated microRNA-21 Reviewed

Koji Nagahama, Kenta Iseda, Daichi Kawano, Junji Kawakami

Biochimie 137 124 - 131 2017.3

Joint Work

Authorship：Lead author

Water Solubilization of Fullerene Derivatives by beta-(1,3-1,6)-D-Glucan and Their Photodynamic Activities toward Macrophages Reviewed International journal

Atsushi Ikeda, Tatsuya Iizuka, Naotake Maekubo, Kazuyuki Nobusawa, Kouta Sugikawa, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Motofusa Akiyama

CHEMISTRY-AN ASIAN JOURNAL 12 ( 10 ) 1069 - 1074 2017.3

Joint Work

Publisher：WILEY-V C H VERLAG GMBH

Anionic and neutral fullerene derivatives were dissolved in water by using beta-(1,3-1,6)-D-glucan (beta-1,3-glucan) as a solubilizing agent. In the water-solubilized complexes, the concentrations of fullerene derivatives were approximate to 0.30 mm and the average particle sizes were approximate to 90 nm. The beta-1,3-glucan-complexed fullerene derivative with a carboxylic acid was found to have higher photodynamic activity toward macrophages under visible-light irradiation (lambda > 610 nm) than other beta-1,3-glucan-complexed fullerene derivatives. This result suggests that carboxylic acid moieties in the complex enhance the binding affinity with beta-1,3-glucan receptors on the surface of macrophages when the beta-1,3-glucan is recognized. In contrast, all beta-1,3-glucan-complexed fullerene derivatives showed no photodynamic activity toward HeLa cells under the same conditions.

DOI： 10.1002/asia.201700182

PubMed

Development of Peptide Microarray to Detect the Protein in Saliva for Periodontal Disease Test Reviewed

Yuki Tominaga, Kenji Usui, Akiyoshi Hirata, Atsushi Kitagawa, Hiro-O Ito and Kiyoshi Nokihara

Peptide Science 2016 131 - 132 2017.3

Joint Work

Formation of beta-(1,3-1,6)-D-glucan-complexed [70]fullerene and its photodynamic activity towards macrophages Reviewed International journal

Atsushi Ikeda, Motofusa Akiyama, Kouta Sugikawa, Kazuya Koumoto, Yuta Kashijima, Jiawei Li, Toshio Suzuki, Takeshi Nagasaki

ORGANIC & BIOMOLECULAR CHEMISTRY 15 ( 9 ) 1990 - 1997 2017.2

Joint Work

Publisher：ROYAL SOC CHEMISTRY

[70] Fullerene was dissolved in water by complexation with beta-1,3-glucan using a mechanochemical highspeed vibration milling apparatus. The photodynamic activity of beta-1,3-glucan-complexed C-70 was highly dependent on the expression level of dectin-1 on the cell surfaces of macrophages. The photodynamic activity increased as a result of a synergistic effect between beta-1,3-glucan-complexed 1 '-acetoxychavicol acetate and the C-70 complex.

DOI： 10.1039/C6OB02747D

PubMed

Cell and Molecular Mechanics in Health and Disease Invited Reviewed

BioMed Research International Article ID 2860241 2017.2

Joint Work

DOI： 10.1155/2017/2860241

Nicotine-Selective Polymeric Adsorbent Obtained by Molecular Imprinting with Excess Use of Itaconic Acid Reviewed

Haruka Nogami, Yuma Nakahori, Takashi Murashima, Jun Matsui

Chromatography 38 15 - 21 2017.1

Joint Work

DOI： 10.15583/jpchrom.2016.017

Substrate stiffness influences doxorubicin- induced p53 activation via ROCK2 expression Reviewed

Ebata T, Mitsui Y, Sugimoto W, Maeda M, Araki K, Machiyama H, Harada I, Sawada Y, Fujita H, Hirata H, Kawauchi K

BioMed Research International 2017.1

Joint Work

Authorship：Lead author

DOI： 10.1155/2017/5158961.

Other Link： https://www.hindawi.com/journals/bmri/2017/5158961/

Unexpected Position-Dependent Effects of Ribose G‑Quartets in G‑Quadruplexes Reviewed

J. Zhou, S. Amrane, F. Rosu, G.F. Salgado, Y. Bian, H. Tateishi-Karimata, E. Largy, D. N. Korkut, A. Bourdoncle, D. Miyoshi, J. Zhang, H. Ju, W. Wang, N. Sugimoto, V. Gabelica, and J. Mergny

J. Am. Chem. Soc. 139 7768 - 7779 2017

Joint Work

Selective and Robust Stabilization of Triplex DNA Structures Using Cationic Comb-type Copolymers. Reviewed

Yamayoshi A, Miyoshi D, Zouzumi YK, Matsuyama Y, Ariyoshi J, Shimada N, Murakami A, Wada T, Maruyama A

J Phys Chem B 121 ( 16 ) 4015 - 4022 2017

Joint Work

Authorship：Lead author

Nicotine-selective Polymeric Adsorbent Obtained by Molecular Imprinting with Excess Use of Itaconic Acid Reviewed

Haruka Nogami, Yuma Nakahori, Takashi Murashima, Jun Matsui

Chromatography 38 ( 1 ) 15 - 21 2017

Authorship：Corresponding author

Currently, nicotine is mostly analyzed by chromatography with pretreatment such as solid phase extraction (SPE). One of effective pretreatment techniques would be affinity extraction; however, there is no practical biomolecular affinity media available for SPE of nicotine. Molecular imprinting has been studied as a methodology for producing nicotine-selective synthetic affinity media, which relies on the formation of complex species between a target molecule (as template) and functional monomer; therefore, the selection of functional monomer is greatly important for obtaining molecularly imprinted polymers (MIPs) with high affinity and selectivity. In this study, itaconic acid (IA), which bears two carboxyl groups, was used as functional monomer in the synthesis of nicotine selective-MIPs. Other acidic monomers, such as methacrylic acid (MA), 2-(trifluoromethyl)acrylic acid (FM) and methyl itaconate (MI) were compared with IA to evaluate their usefulness as functional monomer. In chromatographic tests, the retention factor for nicotine on a MIP synthesized with itaconic acid (IP-IA16) was 40.4, which was 3.1 times that on a non-imprinted polymer (BP-IA16), while the retention factor on MIPs with the other monomers was 21.6 or less and was 1.1 times that on corresponding non-imprint blank polymers (BPs). In the selectivity test using cotinine, 3-methylpyridine and N-methylpyrrolidine as reference compounds, IP-IA16 showed the largest retention factor for nicotine, which was more than 4.4 times that of the other compounds, suggesting that nicotine-recognition sites were formed in MIPs by the molecular imprinting using IA as functional monomer.

Microtubule array observed in the posterior-vegetal cortex during cytoplasmic and cortical reorganization of the ascidian egg. Reviewed

Hirokazu Ishii, Toshiyuki Goto and Takahito Nishikata

Growth and Differentiation 59 648 - 656 2017

Joint Work

DOI： 10.1111/dgd.12405

An integrated function system using metal nanoparticle@mesoporous silica@metal-organic framework hybrids Reviewed

T. Ohhashi, T. Tsuruoka*, K. Inoue, Y. Takashima, S. Horike, K. Akamatsu

Micropor. Mesopor. Mater 245 104 - 108 2017

Joint Work

Rational composition control of mixed-lanthanide metal-organic frameworks by an interfacial reaction with metal ion-doped polymer substrates Reviewed

T. Tsuruoka*, A. Miyanaga, T. Ohhashi, M. Hata, Y. Takashima, K. Akamatsu

J. Solid State Chem 253 43 - 46 2017

Joint Work

An Integrated Function System Using Metal Nanoparticle@Mesoporous Silica@Metal-Organic Framework Hybrids

T. Ohhashi, T. Tsuruoka, K. Inoue, Y. Takashima, S. Horike, K. Akamatsu

Micropor. Mesopor. Mater 245 104 - 108 2017

Joint Work

Rational composition control of mixed-lanthanide metal-organic frameworks by an interfacial reaction with metal ion-doped polymer substrates

T. Tsuruoka, A. Miyanaga, T. Ohhashi, M. Hata, Y. Takashima, K. Akamatsu

J. Solid State Chem 253 43 - 46 2017

Joint Work

Ionic liquid/metal-organic framework hybrid generated by ion-exchange reaction: Synthesis and unique catalytic activity

Y. Takashima, M. Yokoyama, A. Horikoshi, Y. Sato, T. Tsuruoka, K. Akamatsu

New J. Chem 41 14409 - 14413 2017

Joint Work

In situ generation and immobilization of an activated Rh complex catalyst in a metal-organic framework for hydrogenation under low H2 pressure

Y. Takashima, Y. Fukuhara, Y. Sato, T. Tsuruoka, K. Akamatsu

Eur. J. Inorg. Chem 45 5344 - 5349 2017

Joint Work

Influence of intracellular environment on allosteric ribozyme activity

M. Kameno, M. Sawada, N. Sakimoto, J. Kawakami

Proc. 44th Intl. Symp. Nucleic Acid Chemistry 178 - 179 2017

Joint Work

Quantification of stabilization effect of co-solutes on RNA tertiary interaction

N. Sasaki, D. Miyoshi, J. Kawakami

Proc. 44th Intl. Symp. Nucleic Acid Chemistry 174 - 175 2017

Joint Work

An integrated function system using metal nanoparticle@mesoporous silica@metal-organic framework hybrids Reviewed

T. Ohhashi, T. Tsuruoka*, K. Inoue, Y. Takashima, S. Horike, K. Akamatsu

Micropor. Mesopor. Mater 245 104 - 108 2017

Joint Work

Rational composition control of mixed-lanthanide metal-organic frameworks by an interfacial reaction with metal ion-doped polymer substrates Reviewed

T. Tsuruoka*, A. Miyanaga, T. Ohhashi, M. Hata, Y. Takashima, K. Akamatsu

J. Solid State Chem 253 43 - 46 2017

Joint Work

Authorship：Lead author

An integrated function system using metal nanoparticle@mesoporous silica@metal-organic framework hybrids Reviewed

T. Ohhashi, T. Tsuruoka*, K. Inoue, Y. Takashima, S. Horike, K. Akamatsu

Micropor. Mesopor. Mater 245 104 - 108 2017

Joint Work

Ionic Liquid/Metal-Organic Framework Hybrid Generated by Ion-Exchange Reaction: Synthesis and Unique Catalytic Activity Reviewed

Yohei Takashima*, Mai Yokoyama, Atsuto Horikoshi, Yasushi Sato, Takaaki Tsuruoka, Kensuke Akamatsu

New J. Chem. 41 14409 - 14413 2017

Joint Work

Authorship：Lead author

In Situ Generation and Immobilization of an Activated Rh Complex Catalyst in a Metal-Organic Framework for Hydrogenation at Low H2 Pressure Reviewed

Yohei Takashima*, Yoshimasa Fukuhara, Yasushi Sato, Takaaki Tsuruoka, Kensuke Akamatsu

Eur. J. Inorg. Chem 45 5344 - 5349 2017

Joint Work

Authorship：Lead author

Rational composition control of mixed-lanthanide metal-organic frameworks by an interfacial reaction with metal ion-doped polymer substrates Reviewed

T. Tsuruoka*, A. Miyanaga, T. Ohhashi, M. Hata, Y. Takashima, K. Akamatsu

J. Solid State Chem 253 43 - 46 2017

Joint Work

Model studies of the effects of intracellular crowding on nucleic acid interactions Invited Reviewed

13 32 - 41 2016.12

Joint Work

Authorship：Lead author

DOI： 10.1039/c6mb00654j

Elucidation of a “Signal-ON” Mechanism of Aggregation-Induced Emission Dye-Labelled DNA/DNA Duplexes and Application of a Repeat DNA Detection Method Reviewed

Takashi Murashima, Koji Kawamura, Ai Matsumoto

Medicinal Chemistry 6 704 - 709 2016.12

Joint Work

DOI： 10.4172/2161-0444.1000418

Functional characterization of aconitase X as a cis-3-hydroxy-L-proline dehydratase Reviewed

Seiya Watanabe, Kunihiko Tajima, Satoshi Fujii, Fumiyasu Fukumori, Ryotaro Hara, Rio Fukuda, Mao Miyazaki, Kuniki Kino, Yasuo Watanabe

Scientific Reports 6 1 - 12 2016.12

Joint Work

DOI： 10.1038/srep38720

Unusual Colorimetric Change for Alkane Solvents with a Porous Coordination Framework

Takashima Yohei, Sato Yasushi, Tsuruoka Takaaki, Akamatsu Kensuke

INORGANIC CHEMISTRY 55 ( 22 ) 11617 - 11620 2016.11

Joint Work

DOI： 10.1021/acs.inorgchem.6b01754

Effects of trimethylamine N-oxide and urea on DNA duplex and G-quadruplex Reviewed

Y. Ueda, Y. Zouzumi, A. Maruyama, S. Nakano, N. Sugimoto, and D. Miyoshi

Sci. Tech. Adv. Mat. 17 753 - 759 2016.11

Joint Work

DOI： 10.1080/14686996.2016.1243000

細胞核DDSのためのナノトランスポーターの設計 Invited Reviewed

長濱宏治

Drug Delivery System 31 482 - 483 2016.11

Single Work

Authorship：Lead author

Lipid-membrane-incorporated arylboronate esters as agents for boron neutron capture therapy. Reviewed International journal

Masafumi Ueda, Kengo Ashizawa, Kouta Sugikawa, Kazuya Koumoto, Takeshi Nagasaki, Atsushi Ikeda

Organic & biomolecular chemistry 15 ( 7 ) 1565 - 1569 2016.10

Joint Work

Publisher：ROYAL SOC CHEMISTRY

Arylboronate esters bearing methyl groups in both of their ortho positions were stably incorporated into lipid membranes at high concentrations without undergoing hydrolysis to the corresponding boronic acids. This method could be used in combination with previous methods to increase the maximum ratio of boron atoms in liposomal boron carriers.

DOI： 10.1039/C6OB02142E

PubMed

Thermal stability of RNA structures with bulky cations in mixed aqueous solutions Reviewed

S. Nakano, Y. Tanino, H. Hirayama, and N. Sugimoto

Biophys. J. 111 1350 - 1360 2016.10

Joint Work

Authorship：Lead author

DOI： 10.1016/j.bpj.2016.08.031

MEKK1-dependent phosphorylation of calponin-3 tunes cell contractility. Reviewed

Hirata H, Ku WC, Yip AK, Ursekar CP, Kawauchi K, Roy A, Guo AK, Vedula SR, Harada I, Chiam KH, Ishihama Y, Lim CT, Sawada Y, Sokabe M

Journal of Cell Science 129 ( 19 ) 3574 - 3582 2016.10

Joint Work

DOI： 10.1242/jcs.189415

A Systematic Study on Molecular Mechanism of Pore-Forming Peptides for Discovering Antimicrobial Medicine

Y. Sekiya, H. Watanabe, K. Usui, R. Kawano

Proceedings of MicroTAS 2016 595 - 596 2016.10

Joint Work

Increased yield of beta-glucosidase-catalyzed hydrolysis reactions in the presence of betaine-type metabolite analog Reviewed International journal

Shiro Sehata, Ryojun Suzuki, Kazuya Koumoto

BIOPROCESS AND BIOSYSTEMS ENGINEERING 40 ( 1 ) 153 - 159 2016.9

Joint Work

Publisher：SPRINGER

beta-Glucosidases (EC 3.2.1.21), abundant enzymes distributed in animals, plants and microorganism, has been generating lots of attentions for bioethanol production from cellulosic biomass. In this study, using three different origins of beta-glucosidases, glucose productivity of beta-glucosidase-catalyzed hydrolysis reactions in the presence of synthetic betaine-type metabolite analog (2-N,N,N-tri-n-butylammonium) acetate, was investigated. By the addition of the analog, the hydrolysis yields for all beta-glucosidases was highly improved from 4-13 to 64-100 %. To understand the factors affecting on the yield enhancements, the kinetic parameters, inhibition constants of end-product and temporal stability of beta-glucosidases were compared. As a result, enhancement of the yields is mainly related to the increase in the temporal stability of beta-glucosidases in the presence of the analog. The present findings lead to not only improve the glucose productivity of beta-glucosidase-catalyzed hydrolysis reaction toward bioethanol production but also apply to a new stabilization method for various unstable enzymes.

DOI： 10.1007/s00449-016-1684-8

PubMed

Local thermodynamics of the water molecules around single- and double-stranded DNA studied by grid inhomogeneous solvation theory Reviewed

M. Nakano, H. Tateishi-Karimata, S. Tanaka, F. Tamab, O. Miyashita, S. Nakano, and N. Sugimoto

Chem. Phys. Lett. 660 ( 1 ) 250 - 255 2016.9

Joint Work

DOI： 10.1016/j.cplett.2016.08.032

Characterization of functional RNA to perform self-cleavage reaction under low pH condition

N. Sakimoto, M. Kameno, N. Sasaki, J. Kawakami

Proc. 43rd Intl. Symp. Nucleic Acid Chemistry 284 - 285 2016.9

Joint Work

Authorship：Lead author

Discovery of a new function of curcumin which enhances its anticancer therapeutic potency Reviewed

Koji Nagahama, Tomoya Utsumi, Takayuki Kumano, Saeko Maekawa, Naho Oyama, Junji Kawakami

Scientific Reports 6 30962 2016.8

Joint Work

DOI： 10.1038/srep30962

Curcumisome nanovesicles generated by self-assembly of curcumin amphiphiles toward cancer theranostics Reviewed

K. Nagahama, T. Utsumi, T. Kumano, S. Maekawa, N. Oyama, J. Kawakami

Scientific Reports 6 30962 2016.8

Joint Work

Authorship：Lead author

Macrophages Exhibit a Large Repertoire of Activation States via Multiple Mechanisms of Macrophage-activating Factors. Reviewed

Sumiya Y.U., Inoue T., Ishikawa M., Inui T., Kuchiike D., Kubo K., Uto Y., Nishikata T.

Anticancer Res. 36 ( 7 ) 3619 - 3623 2016.7

Joint Work

Authorship：Lead author

Case Report: GcMAF Treatment in a Patient with Multiple Sclerosis. Reviewed

Inui T., Katsuura G., Kubo K., Kuchiike D., Chenery L., Uto Y., Nishikata T., Mette M.

Anticancer Res. 36 ( 7 ) 3771 - 3774 2016.7

Joint Work

Case Report: A Non-small Cell Lung Cancer Patient Treated with GcMAF, Sonodynamic Therapy and Tumor Treating Fields. Reviewed

Inui T., Amitani H., Kubo K., Kuchiike D., Uto Y., Nishikata T., Mette M.

Anticancer Res. 36 ( 7 ) 3767 - 6770 2016.7

Joint Work

Use of a ureido-substituted deoxycytidine module for DNA assemblies Invited Reviewed

S. Nakano, H. Oka, M. Fujii, and N. Sugimoto

Nucleosides, Nucleotides and Nucleic Acids 35 ( 7 ) 370 - 378 2016.5

Joint Work

Authorship：Lead author

DOI： 10.1080/15257770.2016.1174262

A reversible B-A transition of DNA duplexes induced by synthetic cationic copolymers Invited Reviewed

M. Yamaguchi, Y. Zouzumi, N. Shimada, S. Nakano, N. Sugimoto, A. Maruyama, and D. Miyoshi

Chem. Commun. 52 7446 - 7449 2016.4

Joint Work

DOI： 10.1039/C6CC02237E

Site-Specific Control of Silica Mineralization on DNA Using a Designed Peptide Reviewed

M. Ozaki, K. Nagai, H. Nishiyama, T. Tsuruoka, S. Fujii, T. Endoh, T. Imai, K.-y. Tomizaki, K. Usui

Chem. Commun. 52 4010 - 4013 2016.3

Joint Work

Authorship：Lead author

Site-specific control of silica mineralization on DNA using a designed peptide Reviewed

Makoto Ozaki, Kazuma Nagai, Hiroto Nishiyama, Takaaki Tsuruoka, Satoshi Fujii, Tamaki Endoh, Takahito Imai, Kin-ya Tomizaki, Kenji Usui

Chemical Communications 52 ( 21 ) 4010 - 4013 2016.3

Joint Work

DOI： 10.1039/C5CC07870A

Development of Designed Peptides with Secondary Structure for Use in Nanobiotechnology Invited Reviewed

K.Usui

Peptide Science 2015 5 - 8 2016.3

Single Work

Authorship：Lead author

The structural stability and catalytic activity of DNA and RNA oligonucleotides in the presence of organic solvents Invited Reviewed

S. Nakano and N. Sugimoto

Biophys. Rev. 8 ( 1 ) 11 - 23 2016.1

Joint Work

Authorship：Lead author

DOI： 10.1007/s12551-015-0188-0

Reevaluation of the stability of G-quadruplex structures under crowding conditions Reviewed

Jun Zhou, Hisae Tateishi-Karimata, Jean-Louis Mergny, Mingpan Cheng, Zhaochi Feng, Daisuke Miyoshi, Naoki Sugimoto, Can Li

Biochimie 121 204 - 208 2016

Joint Work

A reversible B-A transition of DNA duplexes induced by synthetic cationic copolymers Reviewed

Nonoka Yamaguchi, Yu-ki Zouzumi, Naohiko Shimada, Shuichi Nakano, Naoki Sugimoto, Atsushi Maruyama, Daisuke Miyoshi

Chem. Commun. 52 7446 - 7449 2016

Joint Work

Authorship：Lead author

In situ synthesis of Cu/Ni alloy nanoparticles embedded in thin polymer layers Reviewed

R. Shimizu, T. Kawakami, Y. Takashima, T. Tsuruoka, K. Akamatsu

RSC Adv 6 18895 - 18898 2016

Joint Work

Morphology Control of Metal-Organic Frameworks Based on Paddle-Wheel Units on Ion-Doped Polymer Substrate Using An Interfacial Growth Approach Reviewed

T. Tsuruoka*, K. Mantani, A. Miyanaga, T. Matsuyama, T. Ohhashi, Y. Takashima, K. Akamatsu

Langmuir 32 6068 - 6073 2016

Joint Work

Interfacial Synthetic Approach for Constructing Metal-Organic Framework Crystals Using Metal Ion-Doped Polymer Substrate Reviewed

T. Tsuruoka*, M. Kumano, K. Mantani, T. Matsuyama, A. Miyanaga, T. Ohhashi, Y. Takashima, H. Minami, T. Suzuki, K. Imagawa, K. Akamatsu

Cryst. Growth Des 16 2472 - 2476 2016

Joint Work

Highly Sensitive Plasmonic Optical Sensors Based on Gold Core-Satellite Nanostructures Immobilized on Glass Substrates Reviewed

K. Ode, M. Honjo, Y. Takashima, T. Tsuruoka, K. Akamatsu

ACS Appl. Mater. Interfaces 8 20522 - 20526 2016

Joint Work

Unusual Colorimetric Change for Alkane Solvents with Porous Coordination Framework Reviewed

Y. Takashima, Y. Sato, T. Tsuruoka, K. Akamatsu

Inorg. Chem 55 11617 - 11620 2016

Joint Work

Site-selective growth of metal-organic frameworks using an interfacial growth approach combined with VUV photolithography Reviewed

T. Tsuruoka*, T. Matsuyama, A. Miyanga, T. Ohhashi, Y. Takashima, K. Akamatsu

RSC Adv 6 77297 - 77300 2016

Joint Work

Highly Sensitive Plasmonic Optical Sensors Based on Gold Core−Satellite Nanostructures Immobilized on Glass Substrates

K. Ode, M. Honjo, Y. Takashima, T. Tsuruoka, K. Akamatsu

ACS Appli. Mater. Interfaces 8 20522 - 20526 2016

Joint Work

Unusual Colorimetric Change for Alkane Solvents with a PorousCoordination Framework

Y. Takashima, Y. Sato, T. Tsuruoka, K. Akamatsu

Inorg. Chem 55 11617 - 11620 2016

Joint Work

Site-Selective Growth of Metal–Organic Frameworks Using An Interfacial Growth Approach Combined with VUV Photolithography

T. Tsuruoka, T. Matsuyama, A. Miyanaga, T. Ohhashi, Y. Takashima, K. Akamatsu

RSC Advances 6 77297 - 77300 2016

Joint Work

Morphology Control of Metal-Organic Frameworks Based on Paddle-Wheel Units on Ion-Doped Polymer Substrate Using An Interfacial Growth Approach

T. Tsuruoka, K. Mantani, A. Miyanaga, T. Matsuyama, T. Ohhashi, Y. Takashima, K. Akamatsu

Langmuir 32 6068 - 6073 2016

Joint Work

Interfacial Synthetic Approach for Constructing Metal-Organic Framework Crystals Using Metal Ion-Doped Polymer Substrate

T. Tsuruoka, M. Kumano, K. Mantani, T. Matsuyama, A. Miyanaga, T. Ohhashi, Y. Takashima, H. Minami, T. Suzuki, K. Imagawa, K. Akamatsu

Cryst. Growth Des 16 2472 - 2476 2016

Joint Work

In Situ Synthesis of Cu/Ni Alloy Nanoparticles Embedded in Thin Polymer Layers

R. Shimizu, T. Kawakami, Y. Takashima, T. Tsuruoka, K. Akamatsu

RSC Advances 6 18895 - 18898 2016

Joint Work

Characterization of functional RNA to perform self-cleavage reaction under low pH condition

N. Sakimoto, M. Kameno, N. Sasaki, J. Kawakami

Proc. 43rd Intl. Symp. Nucleic Acid Chemistry 284 - 285 2016

Joint Work

Discovery of a new function of curcumin which enhances its anticancer therapeutic potency

K. Nagahama, T. Utsumi, T. Kumano, S. Maekawa, N. Oyama, J. Kawakami

Scientific Reports 6 30962 2016

Joint Work

A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis. Reviewed

Usui K, Tomizaki KY, Mihara H

Methods in molecular biology (Clifton, N.J.) 1352 199 - 210 2016

DOI： 10.1007/978-1-4939-3037-1_15

PubMed

Site-specific control of silica mineralization on DNA using a designed peptide Reviewed

Makoto Ozaki, Kazuma Nagai, Hiroto Nishiyama, Takaaki Tsuruoka, Satoshi Fujii, Tamaki Endoh, Takahito Imai, Kin-ya Tomizaki, Kenji Usui

CHEMICAL COMMUNICATIONS 52 ( 21 ) 4010 - 4013 2016

Publisher：ROYAL SOC CHEMISTRY

We developed a site-specific method for precipitating inorganic compounds using organic compounds, DNA, and designed peptides with peptide nucleic acids (PNAs). Such a system for site-specific precipitation represents a powerful tool for use in nanobiochemistry and materials chemistry.

DOI： 10.1039/c5cc07870a

PubMed

Site-specific control of multiple mineralizations using a designed peptide and DNA Reviewed

Kenji Usui, Makoto Ozaki, Aoi Yamada, Yoshio Hamada, Takaaki Tsuruoka, Takahito Imai, Kin-ya Tomizaki

NANOSCALE 8 ( 39 ) 17081 - 17084 2016

Publisher：ROYAL SOC CHEMISTRY

We have developed a site-specific method for precipitating multiple inorganic compounds using target DNA and a designed peptide consisting of a peptide nucleic acid (PNA) sequence and an inorganic compound-precipitating sequence. This system for controlled site-specific precipitation represents a powerful tool for use in nanobiotechnology and materials science.

DOI： 10.1039/c6nr03468c

PubMed

Site-specific control of silica mineralization on DNA using a designed peptide Reviewed

M. Ozaki, K. Nagai, H. Nishiyama, T. Tsuruoka, S. Fujii, T. Endoh, T. Imai, K. Tomizaki, K. Usui

Chem. Commun 52 4010 - 4010 2016

Joint Work

Morphology Control of Metal-Organic Frameworks Based on Paddle-Wheel Units on Ion-Doped Polymer Substrate Using An Interfacial Growth Approach Reviewed

T. Tsuruoka*, K. Mantani, A. Miyanaga, T. Matsuyama, T. Ohhashi, Y. Takashima, K. Akamatsu

Langmuir 32 6068 - 6073 2016

Joint Work

Authorship：Lead author

Interfacial Synthetic Approach for Constructing Metal-Organic Framework Crystals Using Metal Ion-Doped Polymer Substrate Reviewed

T. Tsuruoka, M. Kumano, K. Mantani, T. Matsuyama, A. Miyanaga, T. Ohhashi, Y. Takashima, H. Minami, T. Suzuki, K. Imagawa, K. Akamatsu

Cryst. Growth Des 16 2472 - 2476 2016

Joint Work

Authorship：Lead author

In situ synthesis of Cu/Ni alloy nanoparticles embedded in thin polymer layers Reviewed

R. Shimizu, T. Kawakami, Y. Takashima, T. Tsuruoka, K. Akamatsu

RSC Adv 6 18895 - 18898 2016

Joint Work

Unusual Colorimetric Change for Alkane Solvents with Porous Coordination Framework Reviewed

Y.Takashima, Y.Sato,

Inorg.Chem 55 11617 - 11620 2016

Single Work

Site-specific control of multiple mineralizations using a designed peptide and DNA Reviewed

K. Usui, M. Ozaki, A. Yamada, Y. Hamada, T. Tsuruoka, T. Imai, K. Tomizaki

Nanoscale 8 17081 - 17084 2016

Joint Work

Site-selective growth of metal-organic frameworks using an interfacial growth approach combined with VUV photolithography Reviewed

T. Tsuruoka*, T. Matsuyama, A. Miyanga, T. Ohhashi, Y. Takashima, K. Akamatsu

RSC Adv. 6 77297 - 77300 2016

Joint Work

Authorship：Lead author

Highly Sensitive Plasmonic Optical Sensors Based on Gold Core-Satellite Nanostructures Immobilized on Glass Substrates Reviewed

K. Ode, M. Honjo, Y. Takashima, T. Tsuruoka, K. Akamatsu

ACS Appl. Mater.Interfaces 8 20522 - 20526 2016

Joint Work

Functions of the Tumor Suppressors p53 and Rb in Actin Cytoskeleton Remodeling.

Ebata T, Hirata H, Kawauchi K

BioMed research international 2016 9231057 2016

Joint Work

DOI： 10.1155/2016/9231057

PubMed

In situ synthesis of Cu/Ni alloy nanoparticles embedded in thin polymer layers Reviewed

R. Shimizu, T. Kawakami, Y. Takashima, T. Tsuruoka, K. Akamatsu

RSC Adv 6 18895 - 18898 2016

Joint Work

Shrink Wrapping Redox-Active Crystals of Polyoxometalate Open Frameworks with Organic Polymers via Crystal Induced Polymerisation Reviewed

Yohei Takashima, Haralampos N Miras, Stefan Glatzel, Leroy Cronin

Chem. Commun 52 7794 - 7797 2016

Joint Work

Interfacial Synthetic Approach for Constructing Metal-Organic Framework Crystals Using Metal Ion-Doped Polymer Substrate Reviewed

T. Tsuruoka*, M. Kumano, K. Mantani, T. Matsuyama, A. Miyanaga, T. Ohhashi, Y. Takashima, H. Minami, T. Suzuki, K. Imagawa, K. Akamatsu

Cryst. Growth Des 16 2472 - 2476 2016

Joint Work

Morphology Control of Metal-Organic Frameworks Based on Paddle-Wheel Units on Ion-Doped Polymer Substrate Using An Interfacial Growth Approach Reviewed

T. Tsuruoka*, K. Mantani, A. Miyanaga, T. Matsuyama, T. Ohhashi, Y. Takashima, K. Akamatsu

Langmuir 32 6068 - 6073 2016

Joint Work

Highly Sensitive Plasmonic Optical Sensors Based on Gold Core-Satellite Nanostructures Immobilized on Glass Substrates Reviewed

K. Ode, M. Honjo, Y. Takashima, T. Tsuruoka, K. Akamatsu

ACS Appl. Mater. Interfaces 8 20522 - 20526 2016

Joint Work

Unusual Colorimetric Change for Alkane Solvents with Porous Coordination Framework Reviewed

Y. Takashima, Y. Sato, T. Tsuruoka, K. Akamatsu

Inorg. Chem 55 11617 - 11620 2016

Joint Work

Authorship：Lead author

Site-selective growth of metal-organic frameworks using an interfacial growth approach combined with VUV photolithography Reviewed

T. Tsuruoka*, T. Matsuyama, A. Miyanga, T. Ohhashi, Y. Takashima, K. Akamatsu

RSC Adv 6 77297 - 77300 2016

Joint Work

Electrochemical quantification of the Alzheimer's disease amyloid-β(1-40) using amyloid-β fibrillization promoting peptide Reviewed

Satoshi Fujii, Daiki Sono, Kaoru Matsubara, Hitoshi Abe

Sensing and Bio-Sensing Research 6 7 - 12 2015.12

Joint Work

Authorship：Lead author

DOI： 10.1016/j.sbsr.2015.09.001

Facile Quantification of Alzheimer's Disease Amyloid-β Based on Aggregation-Induced Emission Reviewed

Koji Kawamura, Runa Nakayama, Ai Matsumoto, Satoshi Fujii, Takashi Murashima

Journal of Pharmaceutical and Medicinal Research 1 ( 1 ) 27 - 30 2015.12

Joint Work

ATP-Binding Peptide-Hydrogel Composite Synthesized by Molecular Imprinting on Beads Reviewed

Ayana Takata, Kenji Usui, Jun Matsui

Molecular Imprinting 3 65 - 70 2015.12

DOI： 10.1515/molim-2015-0008

Facile Quantification of Alzheimer’s Disease Amyloid-β Based On Aggregation-Induced Emission

Koji Kawamura, Runa Nakayama, Ai Matsumoto, Satoshi Fujii, Takashi Murashima

Journal of Pharmaceutical and Medicinal Research 1 ( 1 ) 27 - 30 2015.11

Joint Work

Thermodynamic properties of water molecules in the presence of cosolute depend on DNA structure: a study using grid inhomogeneous solvation theory Reviewed

M. Nakano, H. Tateishi-Karimata, S. Tanaka, F. Tama, O. Miyashita, S. Nakano, and N. Sugimoto

Nucleic Acids Res. 43 10114 - 10125 2015.11

Joint Work

DOI： 10.1093/nar/gkv1133

A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis Reviewed

K. Usui, K.-y. Tomizaki, H. Mihara

Methods. Mol. Biol. 1352 199 - 210 2015.11

Joint Work

Authorship：Lead author

p53 regulates cytoskeleton remodeling to suppress tumor progression.

Araki K, Ebata T, Guo AK, Tobiume K, Wolf SJ, Kawauchi K

Cellular and molecular life sciences : CMLS 72 ( 21 ) 4077 - 4094 2015.11

Joint Work

DOI： 10.1007/s00018-015-1989-9

PubMed

Facile DNA Detection Based on Fluorescence Switching of A Hydrophobic AIE Dye-Labeled Peptide Nucleic Acid Probe by Aggregation/Disaggregation Reviewed

K. Kawamura, A. Matsumoto, T. Murashima

International Journal of Medical Nano Research 2 ( 2 ) 2015.9

Joint Work

DOI： 10.23937/2378-3664/1410011

Cryoprotective ability of betaine-type metabolite analogs during freezing denaturation of enzymes International journal

Yuichi Nakagawa, Masahiro Sota, Kazuya Koumoto

BIOTECHNOLOGY LETTERS 37 ( 8 ) 1607 - 1613 2015.8

Publisher：SPRINGER

To evaluate an analog library of betaine-type cellular metabolites, which are naturally found in polar fish for survival in subzero temperatures, for preventing denaturation of enzymes during freezing.Comparison of the cryoprotective ability of reported cryoprotectants, such as dimethylsulfoxide, glycerol, ectoine, hydroxyectoine, and trehalose, with betaine-type analogs using alpha-glucosidase revealed that analogs introducing C-3-C-6 alkyl chains into an ammonium cation retained 20 % higher activity than the control cryoprotectants at the same concentration. In particular, the analog possessing triplicate n-butyl chains showed a profound effect. It allowed retention of enzyme activity to 95 % even after 100 freeze-thaw cycles, while addition of the control cryoprotectants decreased the activity to 10-20 %. The cryoprotective ability of betaine-type analogs can be applied not only to alpha-glucosidase but also other enzymes such as beta-glucosidase, alkaline phosphatase, lactose dehydrogenase, sulfatase, and horseradish peroxidase.Synthetic betaine-type metabolite analogs possess practicable cryoprotective ability for various enzymes, and are considerably superior to previously reported cryoprotectants.

DOI： 10.1007/s10529-015-1841-1

PubMed

Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size Reviewed International journal

Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui

BIOCONJUGATE CHEMISTRY 26 ( 8 ) 1775 - 1781 2015.8

Publisher：AMER CHEMICAL SOC

The design and synthesis of materials capable of activating the immune system in a safe manner is of great interest in immunology and related fields. Lactobacilli activate the innate immune system of a host when acting as probiotics. Here, we constructed lactobacilli-mimicking materials in which polysaccharide peptidoglycan complexes (PS PGs) derived from lactobacilli were covalently conjugated to the surfaces of polymeric microparticles with a wide variety of sizes, ranging from 200 nm to 3 mu m. The artificial lactobacilli successfully stimulated macrophages without cytotoxicity. Importantly, we found that the size of artificial lactobacilli strongly influenced their immunostimulating activities, and that artificial lactobacilli of 1 mu m exhibited 10-fold higher activity than natural lactobacilli. One major advantage of the artificial lactobacilli is facile control of size, which cannot be changed in natural lactobacilli. These findings provide new insights into the design of materials for immunology as well as the molecular biology of lactobacillus.

DOI： 10.1021/acs.bioconjchem.5b00319

PubMed

Establishment of a macrophage-activating factor (MAF) assay system using human-monocytic cell line, THP-1. Reviewed

Inoue T., Ishikawa M., Sumiya Y., Kohda H, Inui T., Kuchiike D., Kubo K., Uto Y and Nishikata T.

Anticancer Research 35 4441 - 4445 2015.8

Joint Work

Oral Colostrum Macrophage-activating Factor for Serious Infection and Chronic Fatigue Syndrome: Three Case Reports. Reviewed

Inui T., Kubo K., Kuchiike D., Uto Y., Nishikata T., Sakamoto N., Mette M.

Anticancer Research 35 ( 8 ) 4545 - 4549 2015.8

Joint Work

Macrophage Activation Mechanisms in Human Monocytic Cell Line-derived Macrophages. Reviewed

Sumiya Y, Ishikawa M, Inoue T, Inui T, Kuchiike D, Kubo K, Uto Y, Nishikata T

Anticancer research 35 ( 8 ) 4447 - 4451 2015.8

Joint Work

PubMed

Establishment of a Macrophage-activating Factor Assay System Using the Human Monocytic Cell Line THP-1 Reviewed

Takahiro Inoue, Mami Ishikawa, Yu Sumiya, Haruka Kohda, Toshio Inui, Daisuke Kuchiike, Kentaro Kubo, Yoshihiro Uto, Takahito Nishikata

ANTICANCER RESEARCH 35 ( 8 ) 4441 - 4445 2015.8

Joint Work

Publisher：INT INST ANTICANCER RESEARCH

Background: As the mechanism of macrophage activation is not well-understood, standardization of an assay system for measuring phagocytic activities of macrophages will be useful for research on macrophages. Previously, we established a novel standardized macrophage-activating factor (MAF) assay system using U937. Materials and Methods: Using the human monocytic cell line THP-1, another standardized MAF assay system was established. Characteristic gene expression of U937- and THP-1-derived macrophages was compared by gene expression microarray analysis. Results: Both U937- and THP-1-derived macrophages showed obvious phagocytic activities with unique characteristics and, therefore, could not be assigned to a single sub-type. Conclusion: Activation of macrophages is an intricate cellular process. Comparison of our two novel assay systems provides new insights into macrophage activation mechanisms.

Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size Reviewed

Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, and Jun Matsui

Bioconjugate Chemistry 26 1775 - 1781 2015.8

Joint Work

Authorship：Lead author

Effects of background anionic compounds on the activity of the hammerhead ribozyme in Mg2+-unsaturated solutions Reviewed

S. Nakano, Y. Kitagawa, D. Miyoshi, and N. Sugimoto

J. Biol. Inorg. Chem 20 ( 6 ) 1049 - 1058 2015.7

Joint Work

Authorship：Lead author

DOI： 10.1007/s00775-015-1286-y

Effects of cosolvents on the folding and catalytic activities of the hammerhead ribozyme Reviewed

S. Nakano, Y. Kitagawa, H. Yamashita, D. Miyoshi, and N. Sugimoto

ChemBioChem 16 ( 12 ) 1803 - 1810 2015.7

Joint Work

Authorship：Lead author

DOI： 10.1002/cbic.201500139

生命分子－合成高分子ハイブリッドの開発とナノバイオマテリアル，その可能性 Invited

長濱宏治

Materials Stage 7 18 - 21 2015.7

Single Work

Authorship：Lead author

Anticancer drug-based multifunctional nanogels through self-assembly of dextran-curcumin conjugates toward cancer theranostics Reviewed

Koji Nagahama, Yoshinori Sano, Takayuki Kumano

Bioorganic & Medical Chemistry Letters 25 2519 - 2522 2015.6

Joint Work

Authorship：Lead author

Effects of four-week feed restriction on toxicological parameters in beagle dogs. Reviewed

Takamatsu K., Yamashita H., Satake S., Kazusa K., Tabata H., and Nishikata T.

Experimental Animals 64 269 - 280 2015.5

Joint Work

DOI： 10.1538/expanim.14-0098

A mRNA-responsive G-quadruplex-based drug release system Reviewed

H. Yaku, T. Murashima, D. Miyoshi, N. Sugimoto

Sensors 15 ( 4 ) 9388 - 9403 2015.4

Joint Work

DOI： 10.3390/s150409388

Label and Label-Free Detection Techniques for Protein Microarrays. Reviewed International coauthorship

Syahir A, Usui K, Tomizaki KY, Kajikawa K, Mihara H

Microarrays (Basel, Switzerland) 4 ( 4 ) 228 - 244 2015.4

Single Work

DOI： 10.3390/microarrays4020228

PubMed

DNA G-quadruplex detection system employing a protein fibril ligand Reviewed

R. Maeda, H. Yaku, T. Nakabayashi, T. Murashima, N. Sugimoto, D. Miyoshi

Telomere and Telomerase 2 2015.3

Joint Work

DOI： 10.14800/tt.691

Other Link： http://www.smartscitech.com/index.php/TT/article/view/691/pdf_6

Mechanistic study of unusual enzyme activation caused by addition of inert betaine-type metabolite and the analogs

Yuichi Nakagawa, Kazuya Koumoto

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 249 2015.3

Publisher：AMER CHEMICAL SOC

Self-Assembling Polymer Micelle/Clay Nanodisk/Doxorubicin Hybrid Injectable Gels for Safe and Efficient Focal Treatment of Cancer Reviewed

Koji Nagahama, Daichi Kawano, Naho Oyama, Ayaka Takemoto, Takayuki Kumano, Junji Kawakami

BIOMACROMOLECULES 16 ( 3 ) 880 - 889 2015.3

Joint Work

Publisher：AMER CHEMICAL SOC

The purpose of this study was to fabricate a safe and effective doxorubicin (DOX)-delivery system for focal cancer chemotherapy. A novel biodegradable injectable gel was developed through self-assembly of poly(D,L-lactide-co-glycolide)-b-poly(ethylene glycol)-b-poly(D,L-lactide-co-glycolide) (PLGA-PEG-PLGA) copolymer micelles, clay nanodisks (CNDs), and DOX. We discovered that DOX loaded in the hybrid gels acts as an anticancer drug and as a building block to organize new gel networks. Accordingly, long-term sustained release of DOX from hybrid injectable gels without initial burst release was achieved. Moreover, it was revealed that the DOX incorporated into gel networks controls its own release profile. This hybrid injectable gel is a self-controlled drug release system, which is a novel concept in controlled drug release. Importantly, a single injection of PLGA-PEG-PLGA/CND/DOX hybrid gel provides long-term sustained antitumor activity in vivo against human xenograft tumors in mice, suggesting the potential of hybrid gels as a valuable local DOX-delivery platform for cancer focal therapy.

DOI： 10.1021/bm5017805

Site-Specific Mineralization of Silica and Calcium on DNAs Using a Designed Peptide Reviewed

K. Usui, H. Nishiyama, A. Yamada, M. Ozaki, T. Tsuruoka, K.-y. Tomizaki

Peptide science 2014 325 - 326 2015.3

Joint Work

Authorship：Lead author

Self-Assembling Polymer Micelle/Clay Nanodisk/Doxorubicin Hybrid Injectable Gels for Safe and Efficient Focal Treatment of Cancer Reviewed

Koji Nagahama, Daichi Kawano, Naho Oyama, Ayaka Takemoto, Takayuki Kumano, Junji Kawakami

Biomacromolecules 16 880 - 889 2015.3

Joint Work

Authorship：Lead author

Synthesis and lmmunestimulating Activity of Lactobacilli-Originated Polysaccharide-Polymeric Microparticle Conjugates Reviewed International journal

Koji Nagahama, Takayuki Kumano, Tsubasa Nakata, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui

LANGMUIR 31 ( 4 ) 1489 - 1495 2015.2

Publisher：AMER CHEMICAL SOC

The design and synthesis of biomaterials capable of activating the immune system are of interest in immunology-related fields because of their ability to tune up the immune defenses of the host. Lactobacilli are a major constituent of normal human indigenous flora, and some specific strains are known to activate the immune system of the host as probiotics. In this study, we first fabricated novel biohybrid materials in which lactobacilli (L. casei strain Shirota, LcS)-originated polysaccharide-peptidoglycan complexes (PS-PGs) are conjugated with polymeric microparticles (MPs). PS-PGs conjugated onto polymeric MPs surfaces bound its specific antibody, suggesting that PS-PGs kept their original molecular recognition ability. The PS-PGs-based hybrid MPs with an appropriate density of conjugated PS-PGs effectively induced high levels of IL-12 production from macrophages without cytotoxicity. These results suggest that LcS-originated PS-PGs could be available bio-originated materials for developing novel biomaterials capable of activating the immune system in a safe manner.

DOI： 10.1021/la5041757

PubMed

Synthesis and Immunestimulating Activity of Lactobacilli-Originated Polysaccharide-Polymeric Microparticle Conjugates Reviewed

Koji Nagahama, Takayuki Kumano, Tsubasa Nakata, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, and Jun Matsui

Langmuir 31 1489 - 1495 2015.1

Joint Work

Authorship：Lead author

Synthesis and Immunestimulating Activity of Lactobacilli-Originated Polysaccharide-Polymeric Microparticle Conjugates Reviewed

Koji Nagahama, Takayuki Kumano, Tsubasa Nakata, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui

Langmuir 31 1489 - 1495 2015

Joint Work

Authorship：Lead author

Cryoprotective ability of betaine-type metabolite analogs during freezing denaturation of enzymes Reviewed

Yuichi Nakagawa, Masahiro Sota, Kazuya Koumoto

Biotechnology Letters 37 1607 - 1613 2015

Joint Work

Authorship：Lead author

Immunestimulating Activity of Lactobacilli-mimicking Microparticles modified withPolysaccharide-Peptidoglycan Complex derived from L. casei Shirota Strain Invited

Kazuya Koumoto, Koji Nagahama, Jun Matsui

BIOINDUSTRY 32 ( 9 ) 42 - 47 2015

Joint Work

Authorship：Lead author

Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size Reviewed

Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui

Bioconjugate Chemistry 26 ( 8 ) 1775 - 1781 2015

Joint Work

Authorship：Lead author

Significance of anionic functional group in betaine-type metabolite analogs on the facilitation of enzyme reactions International journal

Yuichi Nakagawa, Kotomi Takagi, Ryutaro Genjima, Kazuya Koumoto

BIOPROCESS AND BIOSYSTEMS ENGINEERING 38 ( 9 ) 1811 - 1817 2015

Joint Work

Authorship：Lead author Publisher：SPRINGER

Using synthetic sulfobetaine library, the enzyme activation behavior has been investigated. Comparison of enzyme activation behavior revealed that sulfobetaines equally facilitate enzyme reactions, being consistent with that of carboxybetaines. The subsequent kinetic and solution property analyses clarified that both the kinetic parameter and hydration property changes are identical with those of carboxybetaines, indicating that the difference in the anionic functional group of the betaine structure scarcely affects the enzyme activation. On the other hand, comparison of carboxy- or sulfo-betaines with tetraalkylammonium salts, whose counteranion binds to the ammonium cation intermolecularly, revealed that the activation ability for enzymes of tetraalkylammonium salts is considerably smaller than that of carboxy- or sulfo-betaines. These findings give us a hint to design the useful betaine-type enzyme activators.

DOI： 10.1007/s00449-015-1422-7

PubMed

Enhanced Chromogenic Sensitivity of Horseradish Peroxidase-Catalyzed Oxidative Reactions in the Presence of Betaine-Type Metabolite Analogs Reviewed

Kotomi Takagi, Yasuhiro Kashima, Satoshi Fujii, Kazuya Koumoto

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 88 ( 8 ) 1074 - 1082 2015

Joint Work

Authorship：Lead author Publisher：CHEMICAL SOC JAPAN

Horseradish peroxidase (HRP), a well-known oxidase, is frequently used in the diagnostic field as a labeling enzyme, where it amplifies the substrate binding signal of antibodies. Though practical for detecting moderate amounts of substrate, there is strong demand for further development of its sensitivity to detect minuscule quantities of biomarkers. Recently, we found that betaine-type cellular metabolite analogs facilitate enzymatic hydrolysis just by dissolving them into the reaction buffer. In the present study, using the analog (2-(N,N,N-tri-n-butylammonium) acetate) and various colorimetric substrates of HRP, we investigated the activation behavior of HRP. As a result, the analog structure- and concentration-dependently facilitated the various HRP-catalyzed oxidative reactions. Interestingly, the analog facilitated not only the reaction rate but also the chromogenic sensitivity. Kinetic and structural analyses revealed that the increased chromogenic sensitivity is related to the enhancement of the conformational flexibility in the substrate binding site in HRP by addition of the analog, which diminishes the binding affinity between HRP and large substrates. The finding serves to create practical applications of the analogs for increased detection sensitivity of HRP-related clinical agents.

DOI： 10.1246/bcsj.20150050

DNA structures under molecular crowding conditions with a phosphorylcholine derivative (MPC) Reviewed

Yu-mi Ueda, Tomohiro Konno, Kazuhiko Ishihara, Naoki Sugimoto, Daisuke Miyoshi

Transact. Mat. Res. Soc. Japan 40 99 - 102 2015

Joint Work

Authorship：Lead author

Effects of background anionic compounds on the activity of the hammerhead ribozyme in Mg2+-unsaturated solutions Reviewed

Shu-ichi Nakano, Yuichi Kitagawa, Daisuke Miyoshi, Naoki Sugimoto

J. Biol. Inorg. Chem. 20 1049 - 1058 2015

Joint Work

Stabilization of DNA Structures With Poly(ethylene sodium phosphate) Reviewed

R. Moriyama, Y. Iwasaki, D. Miyoshi

J. Phys. Chem. B 119 11969 - 11977 2015

Joint Work

Effects of cosolvents on the folding and catalytic activities of the hammerhead ribozyme Reviewed

S. Nakano, Y. Kitagawa, H. Yamashita, D. Miyoshi, N. Sugimoto

ChemBioChem 16 1803 - 1810 2015

Joint Work

A mRNA-responsive G-quadruplex-based drug release system Invited Reviewed

Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, and Naoki Sugimoto

Sensors 15 ( 4 ) 9388 - 9403 2015

Joint Work

Authorship：Lead author

DNA G-quadruplex detection system employing a protein fibril ligand Invited Reviewed

Ryuichi Maeda, Hidenobu Yaku, Takakazu Nakabayashi, Takashi Murashima, Naoki Sugimoto, Daisuke Miyoshi

Telomere and Telomerase 2 e691 2015

Joint Work

Authorship：Lead author

DNA structures under molecular crowding conditions with a phosphorylcholine derivative (MPC)

Yu-mi Ueda, Tomohiro Konno, Kazuhiko Ishihara, Naoki Sugimoto, Daisuke Miyoshi

Transact. Mat. Res. Soc. Japan 40 99 - 102 2015

Joint Work

Stabilization of DNA Structures With Poly(ethylene sodium phosphate)

R. Moriyama, Y. Iwasaki, D. Miyoshi

J. Phys. Chem. B 119 11969 - 11977 2015

Joint Work

Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface Reviewed

T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu

J. Colloid Interface Sci 451 212 - 215 2015

Joint Work

Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface Reviewed

T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu

J. Colloid Interface Sci 451 212 - 215 2015

Joint Work

Metal Nanocrystal/Metal Organic Framework Core/Shgell Nanostructure From Selective Self-Assembly Induced By Localization of Metal Ion Precursors on Nanocrystal Surface

T. Ohhashi, T. Tsuruoka, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu

J. Colloid Interface Sci 451 212 - 215 2015

Joint Work

Comparison of Kinetic and Thermodynamic Analyses for Duplex Formation

E. Tomita, M. Sumimoto, S. Itoh, M. Takashima, and J. Kawakami

Proc. 42nd Intl. Symp. Nucleic Acid Chemistry 142 - 143 2015

Joint Work

Curcumisome nanovesicles generated by self-assembly of curcumin amphiphiles toward cancer theranostics

Koji Nagahama, Takayuki Kumano, Naho Oyama, Junji Kawakami

BIOMATERIALS SCIENCE 3 ( 12 ) 1566 - 1578 2015

Joint Work

Publisher：ROYAL SOC CHEMISTRY

Curcumin (CCM) is an important molecule for achieving cancer theranostics because CCM is a naturally-occurring biocompatible material that exhibits both anticancer activity and strong fluorescence property that can be used for bio-imaging. However, CCM has never been utilized in clinical trials due to its extremely low water solubility, its rapid hydrolysis in aqueous conditions at neutral pH, and its low cellular uptake into cancer cells. Taking advantage of the strong hydrophobicity, p-conjugated frameworks, and ketone and enol groups that generate hydrogen bonds in CCM, we herein fabricated novel CCM-based biodegradable nanovesicles, which we termed as "curcumisome", through the self-assembly of amphiphilic CCM-poly(ethylene glycol) conjugates in aqueous media to develop multifunctional nanobiomaterials for use in cancer theranostics. A high CCM loading content in the curcumisomes was achieved, and the curcumisomes showed high water dispersibility with improved hydrolysis resistance. Importantly, the curcumisomes were effectively internalized into cancer cells and exhibited strong fluorescence for a long period, which is favorable for cancer cell imaging, although only a small amount of the curcumisomes penetrated into normal cells and showed very weak fluorescence. Moreover, curcumisomes effectively induced apoptosis of cancer cells. Thus, curcumisomes may act as multifunctional nanobiomaterials for the development of CCM-based cancer theranostics.

DOI： 10.1039/c5bm00212e

Control of guanine-rich DNA secondary structures depending on the protease activity using a designed PNA peptide Reviewed

Kenji Usui, Arisa Okada, Keita Kobayashi, Naoki Sugimoto

ORGANIC & BIOMOLECULAR CHEMISTRY 13 ( 7 ) 2022 - 2025 2015

Publisher：ROYAL SOC CHEMISTRY

We constructed a regulation system for DNA secondary structure formation of G-rich sequences using a designed PNA peptide exhibiting an on-to-off switching functionality, depending on the protease activity. This study introduces the new concept of a simple and powerful system for regulating quadruplex-related important biological events.

DOI： 10.1039/c4ob02535k

PubMed

Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface Reviewed

T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu

J. Colloid Interface Sci. 451 212 - 215 2015

Joint Work

Site-Specific Mineralization of Silica and Calcium on DNAs Using a Designed Peptide Reviewed

K. Usui, H. Nishiyama, A. Yamada, M. Ozaki, T. Tsuruoka, K.-y. Tomizaki

Peptide Science 325 - 326 2015

Joint Work

Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface Reviewed

T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu

J. Colloid Interface Sci 451 212 - 215 2015

Joint Work

Direct Fabrication of Metal Patterns on Resin Substrate by Combining Imprint and Electrochemical Lithography Reviewed

Yusuke Kimura, Ryosuke Fujiwara, Takaaki Tsuruoka, Yohei Takashima, Hidemi Nawafune, Hiroshi Yanagimoto, Kensuke Akamatsu

J. Surf. Finish. Soc. Jpn 66 18 - 19 2015

Joint Work

Dependence of Crystal Size on the Catalytic Performance of a Porous Coordination Polymer Reviewed

Tomokazu Kiyonaga, Masakazu Higuchi, Takashi Kajiwara, Yohei Takashima, Jingui Duan, Kazuro Nagashima, Susumu Kitagawa

Chem. Commun 51 2728 - 2730 2015

Joint Work

Preparation of hydrogels for the study of the effects of spatial confinement on DNA Reviewed

S. Nakano, M. Yoshida, D. Yamaguchi, and N. Sugimoto

Trans. Mat. Res. Soc. Jpn. 39 435 - 438 2014.12

Joint Work

DOI： 10.14723/tmrsj.39.435

A novel ribozyme core to perform self-cleavage reaction

S. Tanaka, N. Sakimoto, M. Kameno, N. Sasaki, and J. Kawakami

Proc. 41st Intl. Symp. Nucleic Acid Chemistry 340 - 341 2014.11

Joint Work

Authorship：Lead author

Thermodynamic Features Provided by an ENA Introduction to Nucleic Acid Drugs

E. Tomita, M. Sumimoto, and J. Kawakami

Proc. 41st Intl. Symp. Nucleic Acid Chemistry 188 - 189 2014.11

Joint Work

Authorship：Lead author

Effects of oligonucleotide length and locked nucleic acid content on splice-switching oligonucleotide activity

T. Shimo, K. Tachibana, K. Saito, T. Yoshida, E. Tomita, R. Waki, T. Yamamoto, T. Doi, T. Inoue, J. Kawakami, and S. Obika

Proc. 41st Intl. Symp. Nucleic Acid Chemistry 332 - 333 2014.11

Joint Work

Understanding p53: new insights into tumor suppression.

Kawauchi K, Wolf SJ

Expert review of anticancer therapy 14 ( 10 ) 1101 - 1103 2014.10

Joint Work

DOI： 10.1586/14737140.2014.948859

PubMed

Mechanistic study on the facilitation of enzymatic hydrolysis by alpha-glucosidase in the presence of betaine-type metabolite analogs Reviewed

Yuichi Nakagawa, Shiro Sehata, Satoshi Fujii, Hiroaki Yamamoto, Akihiko Tsuda, Kazuya Koumoto

TETRAHEDRON 70 ( 35 ) 5895 - 5903 2014.9

Publisher：PERGAMON-ELSEVIER SCIENCE LTD

Recently we reported that betaine-type metabolite analogs structure-dependently facilitate enzymatic hydrolysis reaction for alpha-glucosidases, beta-glucosidases, and alkaliphosphatases. To understand the facilitation mechanism for enzymes, in this study we expanded the analog library and measured the properties of analog solutions. The structural investigation on alpha-glucosidase-mediated hydrolysis reaction indicated that suitable structures to facilitate the enzyme reaction efficiently should have the ammonium cation in the betaine structure possess triplicate aliphatic chains from Cl to C7 without any polar functional groups. Analyses of the solution properties revealed that such analogs possess a large hydration layer with low water density. Such a specific hydration environment is generated by the characteristic structure of the betaine-type metabolite analogs. The characteristic hydration indirectly regulates enzyme activity and stability. These findings not only increase our understanding enzyme activation by betaine-type metabolite analogs, but also will contribute to the molecular design of enzyme regulators. (C) 2014 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.tet.2014.06.028

Refinement and verification of test conditions for colony-forming unit assay in dog bone marrow cells with preservable colony specimens. Reviewed

Takamatsu K., Wakata A., Hirota R., Tabata H., and Nishikata T.

Comparative Clinical Pathology 23 ( 5 ) 1133 - 1139 2014.9

Joint Work

DOI： 10.1007/s00580-013-1752-2

Cellular differentiation assessments by measuring the degree of cellular internalization and membrane adsorption using designed peptides Reviewed

Kenji Usui, Takuya Kikuchi, Kunio Kikuchi, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 24 ( 17 ) 4129 - 4131 2014.9

Publisher：PERGAMON-ELSEVIER SCIENCE LTD

We demonstrate examples of cellular differentiation assessments, including cellular neurite outgrowth and fat cell maturation, by measuring the degree of membrane adsorption or cellular internalization using designed peptides. Because changes in the cellular membrane and cytosol during differentiation were shown to influence membrane adsorption and cellular internalization, we could successfully evaluate the extent of differentiation simply like stain indicators. (C) 2014 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.bmcl.2014.07.053

PubMed

Roles of the amino group of purine bases in the thermodynamic stability of DNA base pairing Reviewed

S. Nakano and N. Sugimoto

Molecules 19 ( 8 ) 11613 - 11627 2014.8

Joint Work

Authorship：Lead author

DOI： 10.3390/molecules190811613

Case Report: A Breast Cancer Patient Treated with GcMAF, Sonodynamic Therapy and Hormone Therapy. Reviewed

Inui T, Makita K, Miura H, Matsuda A. Kuchiike D, Kubo K, Mette M, Uto Y, Nishikata T, Hori H, and Sakamoto N.

Anticancer Research 34 ( 8 ) 4589 - 4593 2014.8

Joint Work

Dangling ends perturb the stability of RNA duplexes responsive to surrounding conditions Reviewed

H. Tateishi-Karimata, S. Pramanik, S. Nakano, D. Miyoshi, and N. Sugimoto

ChemMedChem 9 ( 9 ) 2150 - 2155 2014.7

Joint Work

DOI： 10.1002/cmdc.201490035

Hammerhead ribozyme activity and oligonucleotide duplex stability in mixed solutions of water and organic compounds Reviewed

S. Nakano, Y. Kitagawa, D. Miyoshi, N. Sugimoto

FEBS Open Bio 4 643 - 650 2014.7

Joint Work

Authorship：Lead author

DOI： 10.1016/j.fob.2014.06.009

Drastic stabilization of parallel DNA hybridizations by a polylysine comb-type copolymer with hydrophilic graft chain Reviewed

D. Miyoshi, Y. Ueda, N. Shimada, S. Nakano, N. Sugimoto, and A. Maruyama

ChemMedChem 9 ( 9 ) 2156 - 2163 2014.7

Joint Work

DOI： 10.1002/cmdc.201402157

A Designed Small Protein For Controlling Site-Specific Mineralization Of Silica And Calcium On Dnas Reviewed

Kenji Usui, Kazuma Nagai, Hiroto Nishiyama, Aoi Yamada, Makoto Ozaki, Takaaki Tsuruoka, Kin-ya Tomizaki

PROTEIN SCIENCE 23 143 - 143 2014.7

Joint Work

Publisher：WILEY-BLACKWELL

Design and evaluation of locked nucleic acid-based splice-switching oligonucleotides in vitro Reviewed

T. Shimo, K. Tachibana, K. Saito, T. Yoshida, E. Tomita, R. Waki, T. Yamamoto, T. Doi, T. Inoue, J. Kawakami and S. Obika

Nucleic Acids Res. 42 8174 - 8187 2014.6

Joint Work

A nanocomposite approach to develop biodegradable thermogels exhibiting excellent cell-compatibility for injectable cell delivery Reviewed

N. Oyama, H. Minami, D. Kawano, M. Miyazaki, T. Maeda, K. Toma, A. Hotta, K. Nagahama

Biomaterials Science 2 ( 8 ) 1057 - 1062 2014.6

Joint Work

Authorship：Lead author

Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers.

Higuchi S, Watanabe TM, Kawauchi K, Ichimura T, Fujita H

Journal of bioscience and bioengineering 117 ( 6 ) 749 - 755 2014.6

Joint Work

DOI： 10.1016/j.jbiosc.2013.11.011

PubMed

Refinement and verification of test conditions for colony-forming unit assay in rat bone marrow cells with preservable colony specimens Reviewed

Takamatsu K., Wakata A., Tabata H., and Nishikata T.

Comparative Clinical Pathology 23 ( 3 ) 569 - 575 2014.5

Joint Work

DOI： 10.1007/s00580-012-1653-9

In vitro assays predictive of telomerase inhibitory effect of G-quadruplex ligands in cell nuclei Reviewed

H. Yaku, T. Murashima, D. Miyoshi, N. Sugimoto

The Journal of Physical Chemistry B 118 ( 10 ) 2605 - 2614 2014.3

Joint Work

DOI： 10.1021/jp410669t

Effects of molecular crowding on the structures, interactions, and functions of nucleic acids Reviewed

S. Nakano, D. Miyoshi, and N. Sugimoto

Chem. Rev. 114 2733 - 2758 2014.3

Joint Work

Authorship：Lead author

DOI： 10.1021/cr400113m

Site-Specific Control of Silica Mineralization on DNA Using Designed Peptides Reviewed

K. Usui, K. Nagai, H. Nishiyama, A. yamada, T. Tsuruoka, S. Fujii, K.-y. Tomizaki

Peptide Science 2013 2415 - 2416 2014.3

Joint Work

Authorship：Lead author

A simple "add and measure" FRET-based telomeric tandem repeat sequence detection and telomerase assay method Reviewed

K. Kawamura, H. Yaku, D. Miyoshi, T. Murashima

Organic & Biomolecular Chemistry 12 ( 6 ) 936 - 941 2014.2

Joint Work

DOI： 10.1039/c3ob42092b

Peptides targeting G-quadruplex structures Reviewed

Kenji Usui, Arisa Okada

Chemical Biology of Nucleic Acids: Fundamentals and Clinical Applications 459 - 475 2014.1

Publisher：Springer Berlin Heidelberg

Research in recent decades has revealed that some DNA and RNA secondary structures modulate a variety of cellular events. One secondary structure, the Guanine(G)-quadruplex, also regulates various cellular events that are mostly related to serious diseases. Systems capable of controlling DNA and RNA G-quadruplex structures would therefore be useful for the modulation of various cellular events to produce biological effects. Because of their biological importance, many G-quadruplex-targeting compounds have been described. However, the next generation of targeting molecules should exhibit increased G-quadruplex sequence specificity, a higher structure-inducing or -collapsing ability, and a greater degree of functionality, including on–off switches of binding ability and cellular penetration. Peptides might be good candidates for these next-generation Gquadruplex- targeting molecules due to the following advantages: (1) their easy design and synthesis, (2) their ability to mimic protein–G–quadruplex interactions, (3) the possibility of employing artificial amino acids in addition to naturally occurring amino acids, and (4) the ability to combine G-quadruplex-binding sequences with other functional sequences. Accordingly, several peptide-based compounds, such as furan-based cyclic peptides, PNA-conjugated peptides, and small molecule-peptide conjugates, have been developed. In this chapter, we introduce all these peptide ligands and describe most of the approaches for targeting G-quadruplex structures. We then conclude that peptides are among the most promising functional ligands for G-quadruplexes to control various biological events in next-generation approaches.

DOI： 10.1007/978-3-642-54452-1_25

Mechanistic study on the facilitation of enzymatic hydrolysis by α-glucosidase in the presence of betaine-type metabolite analogs Reviewed

Yuichi Nakagawa, Shiro Sehata, Satoshi Fujii, Hiroaki Yamamoto, Akihiko Tsuda, Kazuya Koumoto

Tetrahedron 70 5895 - 5903 2014

Joint Work

Authorship：Lead author

Reduced Graphene Oxide Upconversion Nanoparticle Hybrid for Electrochemiluminescent Sensing of a Prognostic Indicator in Early-Stage Cancer Reviewed

Li Wu, Jiasi Wang, Meili Yin, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, Xiaogang Qu

Small 10 330 - 336 2014

Joint Work

A fluorescent probe for detection of an intracellular prognostic indicator in early-stage cancer (10.1039/c4cc08789e Reviewed

Haiwei Ji, Yijia Guan, L Wu, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto Xiaogang Qu

Chem. Commun. 51 1479 - 1482 2014

Joint Work

Preparation of hydrogels for the study of the effects of spatial confinement on DNA Reviewed

Shu-ichi Nakano, Masatoshi Yoshida, Daisuke Yamaguchi, Naoki Sugimoto

Transact. Mat. Res. Soc. Japan 39 435 - 438 2014

Joint Work

Dangling ends perturb the stability of RNA duplex responsive to surrounding condition Invited Reviewed

H. Tateishi-Karimata, S. Pramanik, S. Nakano, D. Miyoshi and N. Sugimoto

ChemMedChem 9 ( 9 ) 2150 - 2155 2014

Joint Work

Hammerhead ribozyme activity and oligonucleotide duplex stability in mixed solutions of water and organic compounds Reviewed

Shu-ichi Nakano, Yuichi Kitagawa, Daisuke Miyoshi, Naoki Sugimoto

FEBS Open Bio 4 643 - 650 2014

Joint Work

Drastic stabilization of parallel DNA hybridizations by a polylysine comb-type copolymer with hydrophilic graft chain Reviewed

Daisuke Miyoshi, Yu-mi Ueda, Naohiko Shimada, Shu-ichi Nakano, Naoki Sugimoto, Atsushi Maruyama

ChemMedChem 9 ( 9 ) 2156 - 2163 2014

Joint Work

Authorship：Lead author

Biosensors Based on Graphene and Biomolecules Invited Reviewed

Bioscience and Bioindustry 72 ( 2 ) 117 - 120 2014

Joint Work

Authorship：Lead author

measure” FRET-based telomeric tandem repeat sequence detection and telomerase assay method Reviewed

Koji Kawamura, Hidenobu Yaku, Daisuke Miyoshi, Takashi Murashima

Org. Biomol. Chem. 12 934 - 941 2014

Joint Work

Effects of Molecular Crowding on the Structures, Interactions, and Functions of Nucleic Acids Reviewed

Shu-ichi Nakano, Daisuke Miyoshi, Naoki Sugimoto

Chem. Rev. 114 ( 5 ) 2733 - 2758 2014

Joint Work

Authorship：Lead author

In Vitro Assays Predicting of Telomerase Inhibitory Effect of G-Quadruplex Ligands in Cell Nuclei Reviewed

Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, Naoki Sugimoto

J. Phys. Chem. B 118 2605 - 2614 2014

Joint Work

Authorship：Lead author

Preparation of hydrogels for the study of the effects of spatial confinement on DNA

Shu-ichi Nakano, Masatoshi Yoshida, Daisuke Yamaguchi, Naoki Sugimoto

Transact. Mat. Res. Soc. Japan 39 435 - 438 2014

Joint Work

Biosensors Based on Graphene and Biomolecules

Bioscience and Bioindustry 72 ( 2 ) 117 - 120 2014

Joint Work

Synthesis and Chromatographic Evaluation of PFOS-selective Polymer by Molecular Imprinting

Rei Sakamoto, Keito Yuasa, Jun Matsui

Chromatography 35 ( Supplement1 ) 81 - 82 2014

Joint Work

Authorship：Lead author

In Situ Synthesis of Metal/Polymer Nanocomposite Thin Films on Glass Substrates by using Highly Cross-Linked Polymer Matrices with Tailorable Ion Exchange Capabilities

Isao Toda, Takaaki Tsuruoka, Jun Matsui, Takashi Murashima, Hidemi Nawafune, Kensuke Akamatsu

RSC Advances 4 4723 - 4726 2014

Joint Work

Synthesis and Chromatographic Evaluation of Affinity Polymer Having Zinc Porphyrin Moieties

Kota Matsuo, Yuki Minetoki, Jun Matsui

Chromatography 35 ( Supplement1 ) 79 - 80 2014

Joint Work

Authorship：Lead author

Mitochondrial inhibitor sodium azide inhibits the reorganization of mitochondria-rich cytoplasm and the establishment of the anteroposterior axis in ascidian embryo Reviewed

Ishii H., Shirai T., Makino C. and Nishikata T

Devl. Growth Differ. 56 175 - 188 2014

Joint Work

DOI： 10.1111/dgd.12117

A Novel Assay System for Macrophage Activating Factor Activity Using a Human U937 Cell Line. Reviewed

Ishikawa M, Inoue T, Inui T, Kuchiike D, Kubo K, Uto Y, and Nishikata T,

Anticancer Research 34 ( 8 ) 4577 - 4581 2014

Joint Work

Simultaneous Expression of Cancer Stem Cell-like Properties and Cancer-associated Fibroblast-like Properties in a Primary Culture of Breast Cancer Cells Reviewed

Ishikawa M, Inoue T, Shirai T, Takamatsu K, Kunihiro S, Ishii H, and Nishikata T

Cancers 6 1570 - 1578 2014

Joint Work

DOI： 10.3390/cancers6031570

A Facile Template Synthesis of Asymmetric Gold Silica Heteronanoparticles Reviewed

R. Miyanohata, T. Matsushita, T. Tsuruoka, H. Nawafune, K. Akamatsu

J. Colloid Interface Sci 416 147 - 150 2014

Joint Work

Electroless nickel plating on polymer particles Reviewed

S. Fujii, H. Hamasaki, H. Takeoka, T. Tsuruoka, K. Akamatsu, Y. Nakamura

J. Colloid Interface Sci 430 47 - 55 2014

Joint Work

Direct fabrication of metal patterns on resin substrate by combining imprint and electrochemical lithography Reviewed

Y. Kimura, R. Fujiwara, T. Tsuruoka, Y. Takashima, H. Nawafune, H. Yanagimoto, K. Akamatsu

Journal of The Surface Finishing Society of Japan 66 18 - 19 2014

Joint Work

Electroless Deposition of Tin Using Contact with Zinc Powder in the Bath Reviewed

E. Uchida, K. Tanaka, A. Kawabata, S. Nabeshima, T. Tsuruoka, K. Akamatsu, H. Nawafune

Journal of The Surface Finishing Society of Japan 65 453 - 457 2014

Joint Work

Fluorescence Enhancement of Immobilized CdTe Nanocrystals on Metal Nanocrystals-Modified Substrate by Dipole Coupling Reviewed

T. Tsuruoka, M. Yamashita, M. Kumano, K. Uwajima, H. Nawafune, K. Akamatsu

Trans. Mater. Res. Soc. Jpn. 39 481 - 484 2014

Joint Work

Gold nanoparticle satellite nanostructures on glass substrate for dual-responsive optical sensing of organic liquids Reviewed

Y. Tanaka, A. Matsumoto, T. Tsuruoka, H. Nawafune, K. Akamatsu

Chem. Lett 43 1084 - 1085 2014

Joint Work

Electroless nickel plating on polymer particles Reviewed

S. Fujii, H. Hamasaki, H. Takeoka, T. Tsuruoka, K. Akamatsu, Y. Nakamura

J. Colloid Interface Sci 430 47 - 55 2014

Joint Work

Direct fabrication of metal patterns on resin substrate by combining imprint and electrochemical lithography Reviewed

Y. Kimura, R. Fujiwara, T. Tsuruoka, Y. Takashima, H. Nawafune, H. Yanagimoto, K. Akamatsu

Journal of The Surface Finishing Society of Japan 66 18 - 19 2014

Joint Work

Electroless Deposition of Tin Using Contact with Zinc Powder in the Bath Reviewed

E. Uchida, K. Tanaka, A. Kawabata, S. Nabeshima, T. Tsuruoka, K. Akamatsu, H. Nawafune

Journal of The Surface Finishing Society of Japan 65 453 - 457 2014

Joint Work

Fluorescence Enhancement of Immobilized CdTe Nanocrystals on Metal Nanocrystals-Modified Substrate by Dipole Coupling Reviewed

T. Tsuruoka, M. Yamashita, M. Kumano, K. Uwajima, H. Nawafune, K. Akamatsu

Trans. Mater. Res. Soc. Jpn. 39 481 - 484 2014

Joint Work

Gold nanoparticle satellite nanostructures on glass substrate for dual-responsive optical sensing of organic liquids Reviewed

Y. Tanaka, A. Matsumoto, T. Tsuruoka, H. Nawafune, K. Akamatsu

Chem. Lett 43 1084 - 1085 2014

Joint Work

Controlled Synthesis of Metal-Organic Framework Films on Metal Nanoparticles by the Versatile Layer-by-Layer Assembly Approach Reviewed

T. Ohhashi, T. Tsuruoka, H. Nawafune, K. Akamatsu

Trans. Mater. Res. Soc. Jpn 39 153 - 156 2014

Joint Work

All‐Inorganic Water-Dispersible Silicon Quantum Dots: Highly Efficient Near-Infrared Luminescence in a Wide pH range

H. Sugimoto, M. Fujii, Y. Fukuda, K. Imakita, K. Akamatsu

Nanoscale 6 122 - 126 2014

Joint Work

Electroless Bismuth Plating as a Peripheral Technology for SiC Power Devices Reviewed

E. Uchida, K. Tanaka, M. Okada, T. Tsuruoka, K. Akamatsu, H. Nawafune

Trans. Mater. Res. Soc. Jpn 39 47 - 51 2014

Joint Work

In Situ Synthesis of Metal/Polymer Nanocomposite Thin Films on Glass Substrates by using Highly Cross-Linked Polymer Matrices with Tailorable ion exchange capabilities Reviewed

I. Toda, T. Tsuruoka, J. Matsui, T. Murashima, H. Nawafune, K. Akamatsu

RSC Advances 4 4723 - 4726 2014

Joint Work

A Facile Template Synthesis of Asymmetric Gold Silica Heteronanoparticles

R. Miyanohata, T. Matsushita, T. Tsuruoka, H. Nawafune, K. Akamatsu

J. Colloid Interface Sci 416 147 - 150 2014

Joint Work

In situ Synthesis of Metal/Polymer Nanocomposite Thin Films on Glass Substrates by using Highly Cross-linked Polymer Matrices with Tailorable Ion Exchange Capabilities

I. Toda, T. Tsuruoka, J. Matsui, T. Murashima, H. Nawafune, K. Akamatsu

RSC Advances 3 16243 - 16246 2014

Joint Work

Gold nanoparticle satellite nanostructures on glass substrate for dual-responsive optical sensing of organic liquids

Y. Tanaka, A. Matsumoto, T. Tsuruoka, H. Nawafune, K. Akamatsu

Chem. Lett 43 1084 - 1085 2014

Joint Work

Fluorescence Enhancement of Immobilized CdTe Nanocrystals on Metal-Nanocrystals-Modified Substrate by Dipole Coupling

T. Tsuruoka, M. Yamashita, M. Kumano, K, Uwajima, Nawafune, K. Akamatsu

Trans. Mater. Res. Soc. Jpn 39 481 - 484 2014

Joint Work

Electroless Bismuth Plating as a Peripheral Technology for SiC Power Devices

E. Uchida, K. Tanaka, M. Okada, T. Tsuruoka, K. Akamatsu, H. Nawafune

Trans. Mater. Res. Soc. Jpn 39 47 - 51 2014

Joint Work

Controlled Synthesis of Metal-Organic Framework Films on Metal Nanoparticles by the Versatile Layer-by-Layer Assembly Approach

T. Ohhashi, T. Tsuruoka, H. Nawafune, K. Akamatsu

Trans. Mater. Res. Soc. Jpn 39 153 - 156 2014

Joint Work

All-Inorganic Water-Dispersible Silicon Quantum Dots: Highly Efficient Near-Infrared Luminescence in a Wide pH range

H. Sugimoto, M. Fujii, Y. Fukuda, K. Imakita, K. Akamatsu

Nanoscale 6 122 - 126 2014

Joint Work

Thermodynamic Features Provided by an ENA Introduction to Nucleic Acid Drugs

E. Tomita, M. Sumimoto, J. Kawakami

Proc. 41st Intl. Symp. Nucleic Acid Chemistry 188 - 189 2014

Joint Work

Mechanistic study on the facilitation of enzymatic hydrolysis by alpha-glucosidase in the presence of betaine-type metabolic analogs Reviewed

Yuichi Nakagawa, Shiro Sehata, Satoshi Fujii, Hiroaki Yamamoto, Akihiko Tsuda, Kazuya Koumoto

Tetrahedron 70 5895 - 5903 2014

Joint Work

Site-Specific Control of Silica Mineralization on DNA Using Designed Peptides Reviewed

K. Usui, K. Nagai, H. Nishiyama, A. Yamada, T. Tsuruoka, S. Fujii, K-y. Tomizaki

Peptide Science 2415 - 2416 2014

Joint Work

Electroless nickel plating on polymer particles Reviewed

S. Fujii, H. Hamasaki, H. Takeoka, T. Tsuruoka, K. Akamatsu, Y. Nakamura

J. Colloid Interface Sci. 430 47 - 55 2014

Joint Work

Direct fabrication of metal patterns on resin substrate by combining imprint and electrochemical lithography Reviewed

Y. Kimura, R. Fujiwara, T. Tsuruoka, Y. Takashima, H. Nawafune, H. Yanagimoto, K. Akamatsu

Journal of The Surface Finishing Society of Japan 66 18 - 19 2014

Joint Work

Electroless Deposition of Tin Using Contact with Zinc Powder in the Bath Reviewed

E. Uchida, K. Tanaka, A. Kawabata, S. Nabeshima, T. Tsuruoka, K. Akamatsu, H. Nawafune

Journal of The Surface Finishing Society of Japan 65 453 - 457 2014

Joint Work

Fluorescence Enhancement of Immobilized CdTe Nanocrystals on Metal Nanocrystals-Modified Substrate by Dipole Coupling Reviewed

T. Tsuruoka, M. Yamashita, M. Kumano, K. Uwajima, H. Nawafune, K. Akamatsu

Trans. Mater. Res. Soc.Jpn 39 481 - 484 2014

Joint Work

Gold nanoparticle satellite nanostructures on glass substrate for dual-responsive optical sensing of organic liquids Reviewed

Y. Tanaka, A. Matsumoto, T. Tsuruoka, H. Nawafune, K. Akamatsu

Chem. Lett 43 1084 - 1085 2014

Joint Work

Controlled Synthesis of Metal-Organic Framework Films on Metal Nanoparticles by the Versatile Layer-by-Layer Assembly Approach Reviewed

T.Ohhashi, T. Tsuruoka, H. Nawafune, K. Akamatsu

Trans. Mater. Res. Soc.Jpn 39 153 - 156 2014

Joint Work

Electroless Bismuth Plating as a Peripheral Technology for SiC Power Devices Reviewed

E. Uchida, K. Tanaka, M. Okada, T. Tsuruoka, K. Akamatsu H. Nawafune

Trans. Mater. Res. Soc.Jpn 39 47 - 51 2014

Joint Work

In Situ Synthesis of Metal/Polymer Nanocomposite Thin Films on Glass Substrates by using Highly Cross-Linked Polymer Matrices with Tailorable ion exchange capabilities Reviewed

I.Toda, T.Tsuruoka, J. Matsui, T. Murashima, H. Nawafune, K. Akamatsu

RSC Adovances 4 4723 - 4726 2014

Joint Work

A Facile Template Synthesis of Asymmetric Gold Silica Heteronanoparticles Reviewed

R. Miyanohata, T. Mstsushita, T. Tsuruoka, H. Nawafune, K. Akamatsu

J. Colloid Interface Sci. 416 147 - 150 2014

Joint Work

Towards Imaging Electron Density inside Metal-Organic Framework Structures Reviewed

Yohei Takashima, De-Liang Long, Leroy Cronin

Chem. Commun 50 2271 - 2274 2014

Joint Work

Enhanced Phosphorescence Emission by Incorporating Aromatic Halides into an Entangled Coordination Framework Based on Naphthalenediimide Reviewed

Virginia Mart?nez-Mart?nez, Rebeca Sola Llano, Shuhei Furukawa, Yohei Takashima, I?igo L?pez Arbeloa, Susumu Kitagawa

ChemPhysChem 15 2517 - 2521 2014

Joint Work

In situ synthesis of metal/polymer nanocomposite thin films on glass substrates by using highly cross-linked polymer matrices with tailorable ion exchange capabilities Reviewed

I. Toda, T. Tsuruoka, J. Matsui, T. Murashima, H. Nawafune, K. Akamatsu

RSC Advances 4 4723 - 4726 2013.12

Joint Work

DOI： 10.1039/C3RA46166A

Control of Site-Specific Silica Precipitation Using PNA Peptides and DNAs Reviewed

K. Usui, H. Nishiyama, K. Nagai, T. Tsuruoka, S. Fujii, K.-y. Tomizaki

Peptides 2013 162 - 163 2013.12

Joint Work

Authorship：Lead author

Designed Peptide Libraries for Cell Analyzing Microarrays Reviewed

H. Mihara, K. Usui, H. Tsutsumi, K. Nokihara

Peptides 2013 22 - 23 2013.12

Joint Work

Peptide-DNA Hybrid G-Quadruplex Structures for Regulation of Protein Expression Depending on Protease Activity Reviewed

A. Okada, M. Taniguchi, K. Usui, N. Sugimoto

Peptides 2013 180 - 181 2013.12

Joint Work

Authorship：Lead author

Cytoplasmic translocation of the retinoblastoma protein disrupts sarcomeric organization.

Araki K, Kawauchi K, Hirata H, Yamamoto M, Taya Y

eLife 2 e01228 2013.12

Joint Work

DOI： 10.7554/eLife.01228

PubMed

Positional Effect of 2',4'-BNA/LNA Introduction into DNA/RNA Duplexes on Thermodynamic Parameters

S. Tahara, E. Tomita, H. Nagai, S. Obika, and J. Kawakami

Proc. 40th Intl. Symp. Nucleic Acid Chemistry 190 - 191 2013.11

Joint Work

Authorship：Lead author

A highly sensitive telomerase activity assay that eliminates false-negative results caused by PCR inhibitors Reviewed

H. Yaku, T. Murashima, D. Miyoshi, N. Sugimoto

Molecules 18 ( 10 ) 11751 - 11767 2013.9

Joint Work

DOI： 10.3390/molecules181011751

Multiple and cooperative binding of fluorescence light-up probe thioflavin t with human telomere DNA G-quadruplex Reviewed

V. Gabelica, R. Maeda, T. Fujimoto, H. Yaku, T. Murashima, N. Sugimoto, D. Miyoshi

Biochemistry 52 ( 33 ) 5620 - 5628 2013.7

Joint Work

DOI： 10.1021/bi4006072

Conformational changes of the phenyl and naphthyl isocyanate-DNA adducts during DNA replication and by minor groove binding molecules Reviewed

S. Nakano, Y. Uotani, Y. Sato, H. Oka, M. Fujii, and N. Sugimoto

Nucleic Acids Res. 41 8581 - 8590 2013.7

Joint Work

Authorship：Lead author

DOI： 10.1093/nar/gkt608

In situ template synthesis of one-dimensional gold nanoparticle arrays in organic nanowires Reviewed

T. Matsushita, Y. Fukumoto, T. Kawakami, T. Tsuruoka, T. Murashima, T. Yanagishita, H. Masuda, H. Nawafune, K. Akamatsu

RSC Advances 3 16243 - 16246 2013.7

Joint Work

DOI： 10.1039/C3RA42727G

Quantitative analyses of nucleic Acid stability under the molecular crowding condition induced by cosolutes Invited Reviewed

Tateishi-Karimata H, Nakano S, Sugimoto N.

Curr Protoc Nucleic Acid Chem. Chapter 7 Unit7.19 2013.6

Joint Work

DOI： 10.1002/0471142700.nc0719s53

Systematic screening of the cellular uptake of designed alpha-helix peptides Reviewed

Kenji Usui, Takuya Kikuchi, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara

BIOORGANIC & MEDICINAL CHEMISTRY 21 ( 9 ) 2560 - 2567 2013.5

Publisher：PERGAMON-ELSEVIER SCIENCE LTD

The cellular penetration (CP) activity of functional molecules has attracted significant attention as one of the most promising new approaches for drug delivery. In particular, cell-penetrating peptides (CPPs) have been studied extensively in cellular engineering. Because there have been few large-scale systematic studies to identify peptide sequences with optimal CP activity or that are suitable for further applications in cell engineering, such as cell-specific penetration and cell-selective culture, we screened and compared the cellular uptake (CU) activity of 54 systematically designed a-helical peptides in HeLa cells. Furthermore, the CU activity of 24 designed peptides was examined in four cell lines using a cell fingerprinting technique and statistical approaches. The CU activities in various cells depended on amino acid residues of peptide sequences as well as charge, a-helical content and hydrophobicity of the peptides. Notably, the mutation of a single residue significantly altered the CU ability of a peptide, highlighting the variability of cell uptake mechanisms. Moreover, these results demonstrated the feasibility of cell-selective culture by conducting cell-selective permeation and death in cultures containing two cell types. These studies may lead to further peptide library design and screening for new classes of CPPs with useful functions. (C) 2013 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.bmc.2013.02.030

PubMed

A peptide release system using a photo-cleavable linker in a cell array format for cell-toxicity analysis Invited Reviewed

Takashi Kakiyama, Kenji Usui, Kin-ya Tomizaki, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara

POLYMER JOURNAL 45 ( 5 ) 525 - 539 2013.5

Joint Work

Authorship：Lead author Publisher：NATURE PUBLISHING GROUP

We constructed a novel peptide-array format system for cellular toxicity analysis. In this system, a peptide was immobilized on a conventional 96-well plate bottom via a photo-cleavable linker. Once UV light irradiated the desired wells, the peptide was released from the bottom. As a result, the cytotoxic behavior of the peptide could be monitored. Immobilization and light-irradiation conditions were optimized. The immobilized peptide showed no cytotoxicity; therefore, the cells could be cultured on the peptide-immobilizing plate from the beginning of the experiment. Cell-toxicity assays with this system for three cell types were performed. All cell types showed similar to 25% lowering of viability with the photo-released 5-(and-6)-carboxytetramethylrhodamine (TMR)-GKLAKLAKKLAKLAKKLAKLAKGC (TMR-KLA-C) peptide compared with the non-coated plate. This relative toxicity nearly corresponded to that of similar to 10 mu M TMR-KLA-C in solution, and we found that the released peptide concentration per well was similar to 10 mu M at 60 min irradiation. Throughout this study, we successfully immobilized peptides via the photo-cleavable linker, released them by UV irradiation spatiotemporally and conducted the cell-toxicity assay. This study implies that the peptide photo-releasing array system will allow the realization of high-throughput cell arrays for cellomics analyses and cell-based phenotypic drug screenings.

DOI： 10.1038/pj.2013.20

Biodegradable polymers exhibiting temperature-responsive sol-gel transition as injectable biomedical materials Reviewed

Koji Nagahama, Akihiro Takahashi, Yuichi Ohya

Reactive and Functional Polymers ( 73 ) 979 - 985 2013.5

Joint Work

Authorship：Lead author

Study on effects of molecular crowding on G-quadruplex-ligand binding and ligand-mediated telomerase inhibition Reviewed

H. Yaku, T. Murashima, H. Tateishi-Karimata, S. –I. Nakano, D. Miyoshi, N. Sugimoto

Methods 64 ( 1 ) 19 - 27 2013.4

Joint Work

DOI： 10.1016/j.ymeth.2013.03.028

A PNA Peptide for the Control of Site-Specific Silica Precipitation on DNA Reviewed

Kenji Usui, Kazuma Nagai, Hiroto Nishiyama, Takaaki Tsuruoka, Satoshi Fujii

Peptide Science 2012 375 - 376 2013.3

Joint Work

Authorship：Lead author

Construction of Artificial Peptides for Control of DNA G-quadruplex Structures Depending on Protease Activity toward Regulation of Protein Expression Reviewed

Arisa Okada, Kenji Usui, Mai Taniguchi, Keita Kobayashi, Naoki Sugimoto

Peptide Science 2012 285 - 286 2013.3

Joint Work

Authorship：Lead author

Enhanced stereocomplex formation of enantiomeric polylactides grafted on a polyrotaxane platform Reviewed

Koji Nagahama, Rie Aoki, Toshifumi Saito, Tatsuro Ouchi, Yuichi Ohya and Nobuhiko Yui

Polymer Chemistry ( 4 ) 1769 - 1773 2013.3

Joint Work

Authorship：Lead author

Design of Biodegradable Injectable Polymers Exhibiting Temperature-Responsive Sol-Gel Transition Reviewed

Y. Ohya, H. Suzuki, K. Nagahama, A. Takahashi, T. Ouchi, A. Kuzuya

Advances in Science and Technology ( 86 ) 9 - 16 2013.3

Joint Work

Thermodynamics - Hydration Relationships within Loops that Affect　G-Quadruplexes under Molecular Crowding Conditions Reviewed

Takeshi Fujimoto, Shu-ichi Nakano, Naoki Sugimoto, Daisuke Miyoshi

J. Phys. Chem. B 117 963 - 972 2013.1

Joint Work

Authorship：Lead author

Thermodynamics-hydration relationships within loops that affect G-quadruplexes under molecular crowding conditions Reviewed

T. Fujimoto, S. Nakano, N. Sugimoto, and D. Miyoshi

J. Phys. Chem. B 117 ( 4 ) 963 - 972 2013.1

Joint Work

Biochemical purification of mitochondrial HSP60 and ATPsynthase from ascidian eggs: Implications for antibody specificity Reviewed

Janet Chenevert, Gerard Pruliere, Hirokazu Ishii, Christian Sardet, Takahito Nishikata

PLoS One 8 ( 1 ) e52996 2013.1

Joint Work

Multiple and cooperative binding of fluorescence light-up probe Thioflavin T with human telomere DNA G-quadruplex Reviewed

Valerie Gabelica, Ryuichi Maeda, Takeshi Fujimoto, Hidenobu Yaku, Takashi Murashima, Naoki Sugimoto, and Daisuke Miyoshi

Biochemistry 52 5620 - 5628 2013

Joint Work

Authorship：Lead author

A Highly Sensitive Telomerase Activity Assay that Eliminates False-Negative Results Caused by PCR Inhibitors Reviewed

Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, and Naoki Sugimoto

Molecules 18 ( 10 ) 11751 - 11767 2013

Joint Work

Authorship：Lead author

Sequence and Solvent Effects on Telomeric DNA Bimolecular G-Quadruplex Folding Kinetics Reviewed

Adrien Marchand, Ruben Ferreira, Hisae Tateishi-Karimata, Daisuke Miyoshi, Naoki Sugimoto, Valerie Gabelica

J. Phys. Chem. B 117 ( 41 ) 12391 - 12401 2013

Joint Work

Recognition of Perfluorooctanoic Acid (PFOA) by Fluorous Molecularly Imprinted Polymer

34 ( Supplement1 ) 21 - 22 2013

Joint Work

Authorship：Lead author

Application of a Reflectometric Interference Spectroscopic Device (RIfS) with a Polymer-gel Coated SiN Chip to an HPLC Detector

Yuma Nakahori, Yusuke Sugahara, Jun Matsui

Chromatography 34 ( Supplement1 ) 51 - 52 2013

Joint Work

Authorship：Lead author

Primary culture of breast cancer: A model system for epithelial-mesenchymal transition and cancer stem cells Reviewed

Nishikata　T., Ishikawa M., Matsuyama T., Takamatsu K., Fukuhara T., and Konishi Y.

Anticancer Research 33 2867 - 2874 2013

Joint Work

Authorship：Lead author

Purification of mitochondrial proteins HSP60 and ATP synthase from ascidian eggs: implications for antibody specificity. Reviewed

Chenevert J, Pruliere G, Ishii H, Sardet C, Nishikata T

PloS one 8 ( 1 ) e52996 2013

Joint Work

DOI： 10.1371/journal.pone.0052996

PubMed

Phosphorus and Boron Codoped Colloidal Silicon Nanocrystals with Inorganic Atomic Ligandas Reviewed

H.Sugimoto, M.Fujii, K.Imakita, S.Hayashi, K.Akamatsu

J.Phys Chem.C. 117 6807 - 6813 2013

Joint Work

Synthesis of Polystyrene-based Nanocomposite Thin Films with Domain Structure Consisting of Au Nanoparticles Reviewed

T. Tsuruoka, S. Kumazaki, I. Osaka, H. Nawafune, K. Akamatsu

Journal of Physics: Conference Series 417 012020 - 012020 2013

Joint Work

In Situ Template Synthesis of One-dimensional Gold Nanoparticle Arrays in Organic Nanowires Reviewed

T. Matsushita, Y. Fukumoto, T. Kawakami, T. Tsuruoka, T. Murashima, T. Yanagishita, H. Masuda, H. Nawafune, K. Akamatsu

RSC Advances 3 16243 - 16246 2013

Joint Work

Codoping n- and p-type impurities in colloidal silicon nanocrystals:controlling luminescence energy from below bulk band gap to visible range

H. Sugimoto, M. Fujii, K. Imakita, S. Hayashi, K Akamatsu

J. Phys. Chem C.117(22) 11850 - 11857 2013

Joint Work

Shape Memory Nanopores Induced in Coordination Frameworks by Crystal Downsizing

Y. Sakata, S. Furukawa, M. Kondo, K. Hirai, N. Horike, Y.Takashima, H. Uehara, N. Louvain, M. Meilikhov, T. Tsuruoka, S. Isoda, W. Kosaka, O. Sakata, S. Kitagawa

Science 339 193 - 196 2013

Joint Work

Phosphorus and Boron Codoped Colloidal Silicon Nanocrystals with Inorganic Atomic Ligands

H. Sugimoto, M. Fujii, K. Imakita, S. Hayashi, K Akamatsu

J. Phys. Chem C.117(13) 6807 - 6813 2013

Joint Work

In Situ Template Synthesis of One-dimensional Gold Nanoparticle Arrays in Organic Nanowires

T. Matsushita, Y. Fukumoto, T. Kawakami, T. Tsuruoka, T. Murashima, T. Yanagishita, H. Masuda, H. Nawafune, K. Akamatsu

RSC Advances 3 16243 - 16246 2013

Joint Work

Specific recognition of cytosine by hypoxanthine in pyrrolidinyl peptide nucleic acid Reviewed

C. Vilaivan, W. Srinarang, N. Yotapan, W. Mansawat, C. Boonlua, J. Kawakami, Y. Yamaguchi, Y. Tanaka, and T. Vilaivan

Org. Biomol. Chem. 11 2310 - 2317 2013

Joint Work

Control of Site-Specific Silica Precipitation Using PNA Peptides and DNAs Reviewed

Kenji Usui, Hiroto Nishiyama, Kazuma Nagai, Takaaki Tsuruoka, Satoshi Fujii, Kin-ya Tomizaki

Peptide Science 162 - 163 2013

Joint Work

A novel array format for monitoring cellular uptake using a photo-cleavable linker for peptide release Reviewed

Kenji Usui, Takuya Kikuchi, Kin-ya Tomizaki, Takashi Kakiyama, Hisakazu Mihara

CHEMICAL COMMUNICATIONS 49 ( 57 ) 6394 - 6396 2013

Publisher：ROYAL SOC CHEMISTRY

We developed a novel peptide array format incorporating a photo-cleavable linker for monitoring cellular uptake. Model peptides were successfully immobilised via the photo-cleavable linker onto conventional plates and could be released spatiotemporally using UV irradiation. Incorporation of confocal microscopy allowed for detailed real-time monitoring of cellular internalisation of peptides.

DOI： 10.1039/c3cc41632a

PubMed

Shape Memory Nanopores Induced in Coordination Frameworks by Crystal Downsizing Reviewed

Y.Sakata, S. Fukumoto, M. Kondo, K. Hirai, N. Horike, Y. Takashima, H.Uehara, N. Louvain, M.Meilikhov, T.Tsuruoka, S.Isoda, W.Kosaka, O.Sakata, S.Kitagawa

Science 339 193 - 196 2013

Joint Work

A PNA Peptide for the Control of Site-Specific Silica Precipitation on DNA Reviewed

K. Usui, K. Nagai, H. Nishiyama, T. Tsuruoka, Satoshi Fujii

Peptide Science 375 - 376 2013

Joint Work

Control of Site-Specific Silica Precipitation Using PNA Peptides and DNAs Reviewed

K. Usui, H. Nishiyama, K. Nagai, T. Tsuruoka, S. Fujii K-y. Tomizaki

Peptide 162 - 162 2013

Joint Work

Shape Memory Nanopores Induced in Coordination Frameworks by Crystal Downsizing Reviewed

Yoko Sakata, Shuhei Furukawa, Mio Kondo, Kenji Hirai, Nao Horike, Yohei Takashima, Hiromitsu Uehara, Nicolas Louvain, Mikhail Meilikhov, Takaaki Tsuruoka, Seiji Isoda, Wataru Kosaka, Osami Sakata, Susumu Kitagawa

Science 339 193 - 196 2013

Joint Work

Base-pairing selectivity of a ureido-linked phenyl-2'-deoxycytidine derivative Reviewed

Nakano S, Oka H, Yamaguchi D, Fujii M, Sugimoto N.

Org Biomol Chem 10 ( 48 ) 9664 - 9670 2012.12

Joint Work

Authorship：Lead author

DOI： 10.1039/c2ob26897c

The beads-on-a-string structure of long telomeric DNAs under molecular crowding conditions Reviewed

Haiqing Yu, Gu Xiaobo Gu, Shu-ichi Nakano, Daisuke Miyoshi, Naoki Sugimoto

J. Am. Chem. Soc. (Higthlighted as the cover and introduced in the Spotslights on Recent JACS Publications) 134 20060 - 20069 2012.12

Joint Work

Authorship：Lead author

The beads-on-a-string structure of long telomeric DNAs under molecular crowding conditions Reviewed

Yu H, Gu X, Nakano S, Miyoshi D, Sugimoto N.

J Am Chem Soc. 134 ( 49 ) 20060 - 20069 2012.12

Joint Work

DOI： 10.1021/ja305384c

Aptamer carbon nanodot sandwich used for fluorescent detection of protein Reviewed

Bailu Xu, Chuanqi Zhao, Weili Wei, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, and Xiaogang Qu

Analyst 137 5483 - 5486 2012.12

Joint Work

Cytosolic subunits of ATP synthase are localized to the cortical endoplasmic reticulum-rich domain of the ascidian egg myoplasm. Reviewed

Hirokazu Ishii, Shiori Kunihiro, Manabu Tanaka, Kyoko Hatano and Takahito Nishikata

Devlopment, Growth and Differentiation 54 753 - 766 2012.12

Joint Work

Construction of a trans-acting system of a L-histidine dependent ribozyme based on the secondary structure

S. Tanaka, Y. Yamaguchi, R. Takamine, and J. Kawakami

Proc. 39th Intl. Symp. Nucleic Acid Chemistry 234 - 235 2012.11

Joint Work

Authorship：Lead author

Myosin II-dependent exclusion of CD45 from the site of Fcγ receptor activation during phagocytosis.

Yamauchi S, Kawauchi K, Sawada Y

FEBS letters 586 ( 19 ) 3229 - 3235 2012.9

Joint Work

DOI： 10.1016/j.febslet.2012.06.041

PubMed

Mechanistic Study on Microstructural Tuning of Metal Nanoparticle/ Polymer Composite Thin Layers: Hydrogenation and Decomposition of Polyimide Matrices Catalyzed by Embedded Nickel Nanoparticles Reviewed

Kensuke Akamatsu , Maki Fujii , Takaaki Tsuruoka , Shu-ichi Nakano , Takashi Murashima , and Hidemi Nawafune

The Journal of Physical Chemistry C 116 ( 33 ) 17947 - 17954 2012.8

Joint Work

DOI： 10.1021/jp304702b

Mechanistic study on microstructural tuning of metal nanoparticle/polymer composite thin layers: Hydrogenation and decomposition of polyimide matrices catalyzed by embedded nickel nanoparticles. Reviewed

K. Akamatsu, M. Fujii, T. Tsuruoka, S. Nakano, T. Murashima, and H. Nawafune

J. Phys. Chem. C 116 17947 - 17954 2012.8

Joint Work

Specific Binding of Anionic Porphyrin and Phthalocyanine to the G-quadruplex Invited Reviewed

Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, Naoki

Molecules 17 10586 - 10613 2012.8

Joint Work

p130Cas-dependent actin remodelling regulates myogenic differentiation.

Kawauchi K, Tan WW, Araki K, Abu Bakar FB, Kim M, Fujita H, Hirata H, Sawada Y

The Biochemical journal 445 ( 3 ) 323 - 332 2012.8

Joint Work

DOI： 10.1042/BJ20112169

PubMed

Dimerization of nucleic acid hairpins in the conditions caused by neutral cosolutes Reviewed

S. Nakano, H. Hirayama, D. Miyoshi, and N. Sugimoto

J. Phys. Chem. B 116 ( 25 ) 7406 - 7415 2012.6

Joint Work

Authorship：Lead author

DOI： 10.1021/jp302170f

Monodispersed Moleculary Imprinted Polymer Beads with Enhanced Atranzine Retention Ability Synthesized with Polymeric Diluents

Tsuyoshi Nagata, Shou Goji, Kensuke Akamatsu, Hidemi Nawafune, Jun Matsui

Analytical Letters 9 572 - 578 2012.6

Joint Work

Colorimetric Detection of Perfluorooctanoic Acid (PFOA) Utilizing Polystyrene-Modified Gold Nanoparticles Reviewed

M. Takayose, K. Akamatsu, H. Nawafune, T. Murashima, J. Matsui

Analytical Letters 45 ( 18 ) 2856 - 2864 2012.5

Joint Work

DOI： 10.1080/00032719.2012.696225

Hydration changes upon DNA folding studied by osmotic stress experiments Reviewed

S. Nakano, D. Yamaguchi, H. Tateishi-Karimata, D. Miyoshi, and N. Sugimoto

Biophys. J. 102 ( 12 ) 2808 - 2817 2012.5

Joint Work

Authorship：Lead author

DOI： 10.1016/j.bpj.2012.05.019

Biodegradable Stereocomplex Materials of Polylactide-grafted Dextran Exhibiting Soft and Tough Properties in Dry and Wet States Reviewed

K. Nagahama, K. Shimizu, S. Ichimura, A. Takahashi, T. Ouchi, Y. Ohya

Journal of Polymer Science Part A: Polymer Chemistry ( 50 ) 2669 - 2676 2012.5

Joint Work

Authorship：Lead author

Biodegradable Polymeric Assemblies for Biomedical Materials Invited Reviewed

Y. Ohya, A. Takahashi, K. Nagahama

Advances in Polymer Science ( 247 ) 65 - 114 2012.4

Joint Work

Authorship：Lead author

Screening of Designed Peptides for Binders to a DNA G-Quadruplex Structure Reviewed

N. Matsui, K. Kobayashi, K. Usui

Peptide Science 2011 2011 315 - 316 2012.3

Joint Work

Authorship：Lead author

Development of PNA-Peptides Controlling DNA G-Quadruplex Structures Depending on Protease Activity Reviewed

K. Usui, K. Kobayashi, N. Sugimoto

Peptide Science 2011 2012 315 - 316 2012.3

Joint Work

Authorship：Lead author

De Novo Designed Nitric Oxide Sensor Protein consisted of Iron Complex-Peptide Conjugate Reviewed

H. Miyazaki, K. Usui, S. Fujii

Peptide Science 2011 2011 303 - 304 2012.3

Joint Work

Chromatin plasticity as a differentiation index during muscle differentiation of C2C12 myoblasts.

Watanabe TM, Higuchi S, Kawauchi K, Tsukasaki Y, Ichimura T, Fujita H

Biochemical and biophysical research communications 418 ( 4 ) 742 - 747 2012.2

Joint Work

DOI： 10.1016/j.bbrc.2012.01.091

PubMed

Detection of a Prognostic Indicator in Early-Stage Cancer Using Functionalized Graphene-Based Peptide Sensors Reviewed

Lingyan Feng, Li Wu, Jiasi Wang, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, Xiaogang Qu

Advanced Materials 24 ( 1 ) 125 - 131 2012.1

Joint Work

Colorimetric Detection of Perfluorooctanoic Acid (PFOA) Utilizing Polystyrene-Modified Gold Nanoparticles Reviewed

Megumi Takayose, Kensuke Akamatsu, Hidemi Nawafune, Takashi Murashima, Jun Matsui

Analytical Letters 2012

Joint Work

Authorship：Lead author

A fluorous synthetic receptor that recognizes perfluorooctanoic acid (PFOA) via fluorous interaction obtained by molecular imprinting Reviewed

Megumi Takayose, Kenji Nishimoto, Jun Matsui

Analyst 2012

Joint Work

Authorship：Lead author

Monodispersed Molecularly Imprinted Polymer Beads with Enhanced Atrazine Retention Ability Synthesized with Polymeric Diluents Reviewed

Tsuyoshi Nagata, Shou Goji, Kensuke Akamatsu, Hidemi Nawafune, Jun Matsui

Analytical Letters 2012

Joint Work

Authorship：Lead author

ポリイミド樹脂/金属間におけるナノ構造界面の構築と樹脂上への金属パターン形成

福本ユリナ、谷山智紀、鶴岡孝章、縄舟秀美、赤松謙祐

エレクトロニクス実装学会誌 15 534 - 540 2012

Joint Work

Mechanistic Study on Microstructural Tuning of Metal Nanoparticle/Polymer Composite Thin Layers:Hydrogenation and Decomposition of Polyimide Matrices Ctalyzed By Embedded Nickel Nanoparticles

K. Akamatsu, M. Fujii, T. Tsuruoka, S. Nakano, T. Murashima, H. Nawafune

J. Phys. Chem. C 116 1794 - 1795 2012

Joint Work

In situ synehesis of composite nanowires consisting of metal nanoparticles in ion-exchangeable polymer matrix using porous alumina tenplates Reviewed

Y.Fukumoto, T.Tsuruoka, T.Yangishita, H.Masuda, H.Nawafune, K. Akamatsu

Ttrans.Mater.Res.Soc.Jpn 37 185 - 188 2012

Joint Work

Synthesis of Au Nanocrustals/Porous Coordination Polymer Composites by Preferential Self-Assembly of Frameworks Reviewed

H.Kawasaki, M.Kumano, M. Yamashita, T. Tsuruoka, H.Nawafune, K.Akamatsu

Trans. Mater. Res. Soc. Jpn 37 181 - 184 2012

Joint Work

Enhancement of upconversion luminescence of AI2O3, Er3+thin films by small assemblies of Au nanorods Reviewed

T.Nakano, M. Fujii, K. Imakita, S. Hayashi, T.Tsuruoka, K. Akamatsu

Trans. Mater. Res. Soc. Jpn 38 449 - 452 2012

Joint Work

Colorimetric Detection of Perfluorooctanoic Acid (PHOA) Utilizing Polystyrene-Modified Gold Nanoparticles

Megumi Takayose, Kensuke Akamatsu, Hidemi Nawafune, Takashi Murashima, Jun Matsui

Analytical Letters 18 2856 - 2864 2012

Joint Work

All‐Inorganic Near-Infrared　Luminescent Colloidal　Silicon Nanocrystals:High Dispersibility in Polar liquid by Phosphorus and Boron Codoping

H. Sugimoto, M. Fujii, K. Imakita, S. Hayashi, K. Akamatsu

J. Phys. Chem. C 116 17969 - 17974 2012

Joint Work

All-Inorganic Near-Infrared Luminescent Colloidal Silicon Nanocrystals:High Dispersibility in Polar Liquid by Phosphorus and Boron Codoping

Fujii, K. Imakita, S. Hayashi, K. Akamatsu

J. Phys. Chem C.116(33) 17969 - 17974 2012

Joint Work

ポリイミド樹脂/金属間におけるナノ構造界面の構築と樹脂上への金属パターン形成

福本ユリナ、谷山智紀、鶴岡孝章、縄舟秀美、赤松謙祐

エレクトロニクス実装学会誌 15 534 - 540 2012

Single Work

Synthesis of Polystyrene-based Nanocomposite Thin Films with Domain Structure Consisting of Au Nano

T. Tsuruoka, S. Kumazaki, I. Osaka, H. Nawafune, K. Akamatsu

Journal of Physics 2012

Joint Work

Synthesis of Au Nanocrystals/Porous Coordination Polymer Composites by Preferential Self-Assembly of Frameworks

H. Kawasaki, M. Kumano, M. Yamashita, T. Tsuruoka, H. Nawafune, K. Akamatsu

Trans. Mater. Res. Soc. Jpn 37 181 - 184 2012

Joint Work

Monodispersed Molecularly Imprinted Polymer Beads with Enhanced Atranzine Retention Ability Synthesized with Polymeric Diluents

Tsuyoshi Nagata, Shou Goji, Kensuke Akamatsu, Hidemi Nawafune, Jun Matsui

Anal. Lett 9 572 - 578 2012

Joint Work

Mechanistic Study on Microstructural Tuning of Metal Nanoparticle/Polymer Composite Thin Layers: Hydrogenation and Decomposition of Polyimide Matrices Ctalyzed By Embedded Nickel Nanoparticles

K. Akamatsu, M. Fujii, T. Tsuruoka, S. Nakano, T. Murashima, H. Nawafune

J. Phys. Chem C.116 1794 - 1795 2012

Joint Work

In situ synthesis of composite nanowires consisting of metal nanoparticles in ion-exchangeable polymer matrix using porous alumina tenplates

Y. Fukumoto, T. Tsuruoka, T. Yangishita, H. Masuda, H. Nawafune, K. Akamatsu

Trans. Mater. Res. Soc. Jpn 37 185 - 188 2012

Joint Work

Enhancement of upconversion Iuminescence of Al2O3 : Er3+, Yb3+ thin films by small assemblies of Au nanorods

T.Nakano, M.Fujii, K. Imakita, S. Hayashi, T. Tsuruoka, K. Akamatsu

Trans. Mater. Res. Soc. Jpn 38 449 - 452 2012

Joint Work

Colorimetric Detection of Perfluorooctanoic Acid (PFOA)Utilizing Polystyrene-Modified Gold Nanoparticles

Megumi Takayose, Kensuke Akamatsu, HIdemi Nawafune, Takashi Murashima, Jun Matsui

Anal. Lett 18 2856 - 2864 2012

Joint Work

Phthalocyanines: A new class of G-quadruplex-ligands with many potential applications（共著） Reviewed

Yaku, H., Fujimoto, T., Murashima, T., Miyoshi, D., Sugimoto, N.

Chemical Communications 48 ( 50 ) 6203 - 6216 2012

Joint Work

Specific binding of anionic porphyrin and phthalocyanine to the G-quadruplex with a variety of in Vitro and in Vivo applications Reviewed

H. Yaku, T. Murashima, D. Miyoshi, N. Sugimoto

Molecules 17 10586 - 10613 2012

Joint Work

DOI： 10.3390/molecules170910586

Mechanistic Study on Microstructural Tuning of Metal Nanoparticle/Polymer Composite Thin Layers:Hydrogenation and Decomposition of Polyimide Matrices Ctalyzed By Embedded Nickel Nanopoarticles Reviewed

K. Akamatsu, M. Fujii, T. Tsuruoka, S. Nakano, T. Murashima, H. Nawafune

J. Phys. Chem. C 2012

Joint Work

Synthesis of Polystyrene-based Nanocomposite Thin Films with Domain Structure Consisting if Au Nanoparticles Reviewed

T. Tsuruoka, S. Kumazaki, I. Osaka, H. Nawafune, K.Akamatsu

J. Physics 2012

Joint Work

ポリイミド樹脂/金属間におけるナノ構造界面の構造と樹脂上への金属パターン形成 Reviewed

福本ユリナ、谷山智紀、鶴岡孝章、縄舟秀美、赤松謙祐

エレクトロニクス実装学会誌 15 534 - 540 2012

Joint Work

Charge Transfer and Exciplex Emissions from a Naphthalenediimide Entangled Coordination Framework accommodating Various Aromatic Guests Reviewed

Virginia Mart?nez-Mart?nez, Shuhei Furukawa, Yohei Takashima, I?igo L?pez Arbeloa, Susumu Kitagawa

J. Phys. Chem 116 26084 - 26090 2012

Joint Work

DNA devises responding to cellular environmental factors Invited

D. Miyoshi

Chemistry and chemical industry (Chemical Society of Japan) 64 ( 12 ) 956 - 956 2011.12

Single Work

Authorship：Lead author

Effects of protein binding on DNA G-quadruplex structures Invited

S. Nagatoishi, D. Miyoshi and N. Sugimoto

Int. Rev. Biophys. Chem. 2 129 - 134 2011.12

Joint Work

Synthesis and Application of Functional Nucleic Acids Invited

Daisuke Miyoshi, Friedrich C. Simmel, Souvik Maiti, and Luis A. Marky

Journal of Nucleic Acids 2011 547245 2011.12

Joint Work

Authorship：Lead author

DNA devises responding to cellular environmental factors

D. Miyoshi

Chemistry and chemical industry (Chemical Society of Japan) 64 ( 12 ) 956 - 956 2011.12

Single Work

Effect of Nickeｌ(II) ion on Copper Electrodeposition from Ethylenediamine Complex Bath

H. Arimura, T. Shigematsu, T. Tsuruoka, K. Akamatsu, H. Nawafune

Journal of The Surface Finishing Society of Japan 62 708 - 711 2011.12

Joint Work

Effect of Nickel(Ⅱ）ion on Copper Electrodeposition from Ethylenediamine Complex Bath

H. Arimura, T. Shigematsu, T. Tsuruoka, K. Akamatsu

Journal of The Surface Finishing Society of Japan 62 708 - 711 2011.12

Joint Work

Rational design of a new IMP aptamer based on a TPP riboswitch and a hypoxanthine aptamer Reviewed

Misato Tokui, Takahiro Yamauchi, Daisuke Miyoshi, Hisaya Kamiya, Akira Nishimura, Naoki Sugimoto

Chemistry Letters 40 ( 11 ) 1313 - 1314 2011.11

Joint Work

Authorship：Lead author

Measurements of the binding of a large protein using a substrate density-controlled DNA chip Reviewed

S. Nakano, T. Kanzaki, M. Nakano, D. Miyoshi, N. Sugimoto

Analytical Chemistry 83 6368 - 6372 2011.11

Joint Work

Structure-function relationship of a histidine dependent ribozyme

Y. Yamaguchi, Y. Ishibashi, S. Tanaka, A. Tanigawa, I. Matsunaga, and J. Kawakami

Proc. 38th Intl. Symp. Nucleic Acids Chemistry 194 - 195 2011.11

Joint Work

Authorship：Lead author

Cell fingerprint patterns using designed alpha-helical peptides to screen for cell-specific toxicity Reviewed

Kenji Usui, Takashi Kakiyama, Kin-ya Tomizaki, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 21 ( 21 ) 6281 - 6284 2011.11

Publisher：PERGAMON-ELSEVIER SCIENCE LTD

We conducted cell-based cytotoxicity screening of a 101-membered alpha-helical peptide library using cell fingerprints (CFPs). The CFP data suggested that there is a relationship between cytotoxicity and peptide characteristics, such as hydrophobicity, charge, and amino acid composition. In spite of the small size of the library used in this study, several peptides demonstrated cell-specific toxicity. The strategy of combining a designed peptide library with CFP thus shows real promise for peptide-based screening with cells. (C) 2011 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.bmcl.2011.09.002

PubMed

Triplet analysis that identifies unpaired regions of functional RNAs（共著） Reviewed

J. Kawakami, Y. Yamaguchi, and N. Sugimoto

Journal of Nucleic Acids 2011 doi:10.4061/2011/471843 2011.10

Joint Work

Authorship：Lead author

Other Link： http://www.hindawi.com/journals/jna/2011/471843/

The effects of molecular crowding on the structure and stability of G-quadruplexes with an abasic site Invited Reviewed

T. Fujimoto, S. Nakano, D. Miyoshi, and N. Sugimoto

Journal of Nucleic Acids (Hindawl Publishing Corporation) 2011 ( 857149 ) 857149 2011.9

Joint Work

DOI： 10.4061/2011/857149

A Novel Nitric Oxide Sensor Using Fluorescenct Peptides Attached to Iron Complexes

Hiroshi Miyazaki, Kenji Usui, Satoshi Fujii

Peptides: Building Bridges. Proceeding of the Twenty-Second American Peptide Symposium 224 - 225 2011.9

Joint Work

Graphene biosensors Invited

D. Miyoshi, N. Sugimoto

Chemistry 66 ( 8 ) 30 - 35 2011.9

Joint Work

Authorship：Lead author

The effects of molecular crowding on the structure and stability of G-quadruplexes with an abasic site Reviewed

Takeshi Fujimoto, Shu Ichi Nakano, Daisuke Miyoshi, and Naoki Sugimoto

J. Nucleic Acids 2011 857149 2011.9

Joint Work

Authorship：Lead author

Graphene biosensors

D. Miyoshi, N. Sugimoto

Chemistry 66 ( 8 ) 30 - 35 2011.9

Joint Work

Fabrication of Silver Patterns on Polyimide Films Based on Solid-Phase Electrochemical Constructive Lithography Using Ion-Exchangeable Precursor Layers

K. Akamatsu, Y. Fukumoto, T. Taniyama, T. Tsuruoka, H. Yanagimoto, H. Nawafune

Langmuir 27 11761 - 11766 2011.9

Joint Work

Contlloed Self-Assembly of Metal-Organic Frameworks on Metal Nanoparticles for Efficient Synthesis of Hybrid Nanostructures

T. Tsuruoka, H. Kawasaki, H. Nawafune, K. Akamatsu

Contlloed Self-Assembly of Metal-Organic Frameworks on Metal Nanoparticles for Efficient Synthesis of Hybrid Nanostructures 3 3788 - 3791 2011.9

Joint Work

Contlloed Self-Assembly of　Metaｌ-Organic Frameworks on Metal Nanoparticle for Efficient Synthesis of Hybrid Nanostructures Reviewed

T.Tsuruoka, H.Kawasaki, H. Nawafune, K. Akamatsu

ACS Appl. Mater. Interfaces 3 3788 - 3791 2011.9

Joint Work

Fabrication of Silver Patterns on Polyimide Films Based on Solid-Phase Electrochemical Constructive Lithography Using Ion-Exchangeable Precursor Layers

K. Akamatsu, Y. Fukumoto, T. Taniyama, T. Tsuruoka, H.Yanagimoto, H. Nawafune

Langmuir 27 11761 - 11766 2011.9

Joint Work

Measurements of the binding of a large protein using a substrate density-controlled DNA chip Reviewed

S. Nakano, T. Kanzaki, M. Nakano, D. Miyoshi, and N. Sugimoto

Anal. Chem. 83 ( 16 ) 6768 - 6372 2011.8

Joint Work

Authorship：Lead author

DOI： 10.1021/ac201312d

Effect of locked nucleic acid modifications on the thermal stability of noncanonical DNA structure Reviewed

J. Bhattacharyya, S. Maiti, S. Muhuri, S. Nakano, D. Miyoshi, and N. Sugimoto

Biochemistry 50 ( 34 ) 7414 - 7425 2011.8

Joint Work

DOI： 10.1021/bi200477g

Label-free colorimetric and quantitative detection of cancer marker protein using noncrosslinking aggregation of Au/Ag nanoparticles induced by target-specific peptide probe Reviewed

Xiaohui Wang, Li Wu, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, and Xiaogang Qu

Biosensors and Bioelectronics 26 4804 - 4809 2011.8

Joint Work

In situ generation of silver nanoparticles in ion-exchangeable polymer photonic crystals

T. Tsuruoka, S. Kumazaki, H. Nawafune, K. Akamatsu

Chem. Lett. 40 1233 - 1234 2011.8

Joint Work

In situ generation of silver nanoparticles in ion-exchangeable polymer photonic crystals Reviewed

T.Tsuruoka, S.Kumazaki, H. Nawafune, K. Akamatsu

Chem. Lett. 40 1233 - 1234 2011.8

Joint Work

Use of nucleic acid analogs for the study of nucleic acid interactions Invited Reviewed

S. Nakano, M. Fujii, and N. Sugimoto

Journal of Nucleic Acids 2011 ( 967098 ) 967098 2011.7

Joint Work

DOI： 10.4061/2011/967098

Monolayer-Functionalized CｄTe Nanocrystals with Controlled Fluorescent Properties Based on Chemical Surface Modification

T.Tsuruoka, M.Sugiyama, R.Takahashi, H. Nawafune, K.Akamatsu

Trans.Mater.Res.Soc.Jpn 36 183 - 186 2011.6

Joint Work

Cellular zwitterionic metabolite analogs simultaneously enhance reaction rate, thermostability, salt tolerance, and substrate specificity of alpha-glucosidase Reviewed

Eisuke Deguchi, Kazuya Koumoto

Bioorganic and Medicinal Chemistry 19 3128 - 3134 2011.4

Joint Work

Authorship：Lead author

天然の代謝産物を模倣し、化学的に合成した代謝産物アナログがα−グルコシダーゼ、β−グルコシダーゼ、アルカリフォスファターゼといった加水分解酵素の活性（反応速度、耐熱性、耐塩性、基質選択性等）を溶液に共存させるだけで向上させることを明らかとした。

DOI： 10.1016/j.bmc.2011.04.003

Thermodynamic stability of Hoogsteen and Watson-Crick base pairs in the presence of histone H3-mimicking peptide Reviewed

S. Pramanik, K. Nakamura, K. Usui, S. Nakano, S. Saxena, J. Matsui, D. Miyoshi and N. Sugimoto

Chem. Commun. 47 ( 10 ) 2790 - 2792 2011.2

Joint Work

DOI： 10.1039/C0CC05776B

Thermodynamic Stability of Hoogsteen and Watson-Crick Base Pairs in the Presence of Histone H3-Mimicking Peptide Reviewed

Smritimoy Pramanik, Kaori Nakamura, Kenji Usui, Shu-ichi Nakano, Sarika Saxena, Jun Matsuia, Daisuke Miyoshi, Naoki Sugimoto

Chem. Commun. 47 2790 - 2792 2011.2

Joint Work

Authorship：Lead author

Biodegradable Nano-aggregates of Star-Shaped 8-arm PEG-PLLA Block Co-polymers for Encapsulation of Water-Soluble Macromolecules Reviewed

K. Nagahama, T. Saito, T. Ouchi, Y. Ohya

Journal of Biomaterials Science, Polymer Edition 22 407 2011.1

Joint Work

Authorship：Lead author

Thermodynamic Stability of Hoogsteen and Watson-Crick Base Pairs in the Presence of Histone H3-Mimicking Peptide Reviewed

Smritimoy Pramanik, Kaori Nakamura, Kenji Usui, Shu-ichi Nakano, Sarika Saxena, Jun Matsui, Daisuke Miyoshi, Naoki Sugimoto

Chemical Communications 2011

Joint Work

Direct Detection of Thrombin Binding to 8-Bromodeoxyguanosine-Modified Aptamer: Effects of Modification on Affinity and Kinetics Reviewed

S. Goji, J. Matsui

Journal of Nucleic Acids 2011

Joint Work

Authorship：Lead author

Monolayer-Functionalized CdTe Nanocrystals with Controlled Fluorescent Properties Based on Chemical Surface Modification

T. Tsuruoka, M. Sugiyama, R. Takahashi, H. Nawafune, K. Akamatsu

Trans. Mater. Res. Soc. Jpn 36 183 - 186 2011

Joint Work

Synthesis of Au Nanocrystals/Porous Coordination Polymer Compoosites by Preferential Self-Assembly of Frameworks

H. Kawasaki, M. Kumano, M. Yamashita, T. Tsuruoka, H. Nawafune, K. Akamatsu

Trans. Mater. Res. Soc. Jpn. 37 181 - 184 2011

Joint Work

In situ synthesis of　comosite nanowires consisting of metal nanoparticles in ion-exchangeable polymer matrix using porous alumina tenplates

Y. Fukumoto, T. Tsuruoka, T.Yanagishita, H. Masuda, H. Nawafune, K. Akamatsu

Trans. Mater. Res. Soc. Jpn. 37 185 - 188 2011

Joint Work

Contlloed Self-Assembly of Metal-Organic Frameworks on Metal Nanoparticles for Efficient Synthesis of Hybrid Nanostructures

T. Tsuruoka, H. Kawasaki, H. Nawafune, K. Akamatsu

ACS Appl. Mater. Interfaces 3 3788 - 3791 2011

Joint Work

Morphology Design of Porous Coordination Polymer Crystals by Coordination Modulation

A. Umemura, S. Diring, S. Furukawa, H. Uehara, T. Tsuruoka, S. Kitagawa

J. Am.Chem.Soc 133 15506 - 15513 2011

Joint Work

Monolayer-Functionalized CdTe Nanocrystals with Controlled Fluorescent Properties Based on Chemical Surface Modification

T. Tsuruoka, M. Sugiyama, R. Takahashi, H. Nawafune, K. Akamatsu

Trans. Mater. Res. Soc. Jpn 36 183 - 186 2011

Joint Work

In situ generation of silver nanoparticles in ion-exchangeable polymer photonic crystals

T. Tsuruoka, S. Kumazaki, H. Nawafune, K. Akamatsu

Chem. Lett 40 1233 - 1234 2011

Joint Work

Fabrication of Silver Patterns on Polyimide Films Based on Solid-Phase Electrochemical Constructive Lithography Using Ion-Exchangeable Precursor Layers

K. Akamatsu, Y. Fukumoto, T. Taniyama, T. Tsuruoka, H. Yanagimoto, H. Nawafune

Langmuir 27 11761 - 11766 2011

Joint Work

Effect of Nickel(II) ion on Copper Electrodeposition from Ethylenediamine Complex Bath

H. Arimura, T. Shigematsu, T. Tsuruoka, K. Akamatsu, H. Nawafune

Journal of The Surface Finishing Society of Japan 62 708 - 711 2011

Joint Work

Pyrrolidinyl peptide nucleic acid with α/β-peptide backbone - A conformationally constrained PNA with unusual hybridization properties

C. Vilaivan, C. Srisuwannaket, C. Ananthanawat, C. Suparpprom, J. Kawakami, Y. Yamaguchi, Y. Tanaka, T. Vilaivan

Artificial DNA: PNA & XNA 2 11 2011

DOI： 10.4161/adna.2.2.16340

Thermodynamic stability of Hoogsteen and Watson-Crick base pairs in the presence of histone H3-mimicking peptide Reviewed International coauthorship

Smritimoy Pramanik, Kaori Nakamura, Kenji Usui, Shu-ichi Nakano, Sarika Saxena, Jun Matsui, Daisuke Miyoshi, Naoki Sugimoto

CHEMICAL COMMUNICATIONS 47 ( 10 ) 2790 - 2792 2011

Publisher：ROYAL SOC CHEMISTRY

We found that Hoogsteen base pairs were stabilized by molecular crowding and a histone H3-mimicking peptide, which was not observed for Watson-Crick base pairs. Our findings demonstrate that the type of DNA base pair is critical for the interaction between DNA and histones.

DOI： 10.1039/c0cc05776b

PubMed

Use of a designed peptide library to screen for binders to a particular DNA G-quadruplex sequence Reviewed

Keita Kobayashi, Noriko Matsui, Kenji Usui

Journal of Nucleic Acids 2011 572873 2011

We demonstrated a method to screen for binders to a particular G-quadruplex sequence using easily designed short peptides consisting of naturally occurring amino acids and mining of binding data using statistical methods such as hierarchical clustering analysis (HCA). Despite the small size of the library used in this study, candidates of specific binders were identified. In addition, a selected peptide stabilized the G-quadruplex structure of a DNA oligonucleotide derived from the promoter region of the protooncogene c-MYC. This study illustrates how a peptide library can be designed and presents a screening guideline for construction of G-quadruplex binders. Such G-quadruplex peptide binders could be functionally modified to enable switching, cellular penetration, and organelle-targeting for cell and tissue engineering. © 2011 Keita Kobayashi et al.

DOI： 10.4061/2011/572873

PubMed

PNA-Peptide Conjugates for Regulation of DNA and RNA G-Quadruplex Structures Depending on a Particular Protease Concentration Reviewed

Kenji Usui, Keita Kobayashi, Naoki Sugimoto

Peptides 2010 2010 608 - 609 2011

Joint Work

Authorship：Lead author

Nanomolar aggregation of amyloid-beta peptides covalently and site-specifically modified by cholesterol oxidation products Reviewed International coauthorship

Kenji Usui, Evan T. Powers, Johan F. Paulsson, Sarah J. Siegel & Jeffery W. Kelly

Peptides 2008 2008 588 - 589 2011

Joint Work

Authorship：Lead author

Fluorescent and Luminescent Fusion Proteins for Detection of Amyloid Beta Peptide Localization and Aggregation Reviewed

Kenji Usui, Masayasu Mie, Takashi Andou, Naoki Sugimoto, Hisakazu Mihara, Eiry Kobatake

Peptides 2010 2010 488 - 489 2011

Joint Work

Authorship：Lead author

Designed Peptide Libraries for Protein and Cell analyses Reviewed

K. Kikuchi, K. Usui, K.-Y. Tomizaki, T. Takahashi, H. Mihara

Peptide Science 2010 2010 21 - 21 2011

Joint Work

A Novel Nitric Oxide Sensor Using Fluorescent Peptides Attached to Iron Complexes Reviewed

Hiroshi Miyazaki, Kenji Usui, Satoshi Fujii

Peptides 2011 2011 224 - 255 2011

Joint Work

A PNA Peptide Inducing DNAs to Form G-Quadruplex Structures Depending on a Protease Activity Reviewed

Kenji Usui, Keita Kobayashi, Naoki Sugimoto

Peptides 2011 2011 152 - 153 2011

Joint Work

Authorship：Lead author

Nanomolar Aggregation and Neurotoxicity of Amyloid beta Peptides with Site-Specific Modification of Oxidized Cholesterol Reviewed International coauthorship

K. Usui, J. D. Hulleman, J. F. Paulsson, S. J. Siegel, E. T. Powers, J. W. Kelly

Peptide Science 2010 2010 169 - 169 2011

Joint Work

Authorship：Lead author

Synthesis of Au Nanocrystals/Porous Coordination Polymer Compoosites by Perferential Self-Assembly of Frameworks Reviewed

H. Kawasaki, M. Kumano, M. Yamashita, T. Tsuruoka, H. Nawafune, K. Akamatsu

Trans. Mater. Res. Soc. Jpn. 37 181 - 184 2011

Joint Work

In situ synthesis of　comosite nanowires consisting of metal nanoparticles in ion-exchangeable polymer matrix using porous alumina tenplates

Y. Fukumoto, T. Tsuruoka, T.Yanagishita, H. Masuda, H. Nawafune, K. Akamatsu

Trans. Mater. Res. Soc. Jpn. 37 185 - 188 2011

Joint Work

Molecular Decoding using Luminescence from an Entangled Porous Framework Reviewed

Yohei Takashima, Virginia Mart?nez Mart?nez, Shuhei Furukawa, Mio Kondo, Satoru Shimomura, Hiromitsu Uehara, Masashi Nakahama, Kunihisa Sugimoto, Susumu Kitagawa

Nature Commun 2 168 - 168 2011

Joint Work

Thermodynamically controlled coordination-engineering of novel 2D cadmium thiolate coordination polymers Reviewed

Nicolas Louvain, Yohei Takashima, Susumu Kitagawa, Shuhei Furukawa

New J. Chem. 35 1265 - 1269 2011

Joint Work

Control of the Charge-Transfer Interaction between a Flexible Porous Coordination Host and Aromatic Guests by Framework Isomerism Reviewed

Yohei Takashima, Shuhei Furukawa, Susumu Kitagawa

CrystEngComm 13 3360 - 3363 2011

Joint Work

Ultrasensitive and Selective Detection of a Prognostic Indicator in Early-Stage Cancer Using Graphene Oxide and Carbon Nanotubes Reviewed

Xiaohui Wang, Chunyan Wang, Konggang Qu, Yujun Song, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, and Xiaogang Qu

Advanced Functional Materials 20 3967 - 3971 2010.12

Joint Work

Comparative thermodynamic analysis of RNA-protein interaction on surface and in solution（共著）

Y. Tanaka, K. Ishidate, K. Kishimoto, N. Sugimoto, and J. Kawakami

Proc. 37th Intl. Symp. Nucleic Acids Chemistry 276 - 277 2010.11

Joint Work

Authorship：Lead author

A rapid and sensitive "add-mix-measure" assay for multiple proteinases based on one goldnanoparticle-peptide-fluorophore conjugate Reviewed

X.Wang, J.Geng, D.Miyoshi, J.Ren, N.Sugimoto, and X.Qu

Biosens. Bioelectron. 26 743 - 747 2010.11

Joint Work

Stacking interaction in the middle and at the end of a DNA helix studied with non-natural nucleotides Reviewed

S. Nakano, H. Oka, Y. Uotani, K. Uenishi, M. Fujii, and N. Sugimoto

Mol. BioSyst. 6 2023 - 2029 2010.10

Joint Work

DOI： 10.1039/C0MB00002G

Sole and stable RNA duplexes of G-rich sequences located in the 5'-untranslated region of protooncogenes Reviewed

Sarika Saxena, Daisuke Miyoshi, Naoki Sugimoto

Biochemistry 49 7190 - 7201 2010.9

Joint Work

Authorship：Lead author

Development of new functional nanostructures consisting of both DNA duplex and quadruplex Reviewed

Kakoli Dutta, Takeshi Fujimoto, Mamiko Inoue, Daisuke Miyoshi, and Naoki Sugimoto

Chem. Commun 46 7772 - 7774 2010.9

Joint Work

Authorship：Lead author

Controlled Multiscale Synthesis of Porous Coordination Polymer in Nano/Micro Regimes

Diring Stephane, Furukawa Shuhei, Takashima Yohei, Tsuruoka Takaaki, Kitagawa Susumu

CHEMISTRY OF MATERIALS 22 ( 16 ) 4531 - 4538 2010.8

Joint Work

DOI： 10.1021/cm101778g

Anionic phthalocyanines targeting G-quadruplexes and inhibiting telomerase activity in the presence of excessive DNA duplexes Reviewed

Yaku, H., Murashima, T., Miyoshi, D., Sugimoto, N.

Chemical Communications 46 ( 31 ) 5740 - 5742 2010.8

Joint Work

Anionic Phthalocyanines Targeting G-quadruplexes and Inhibiting Telomerase Activity in the Presence of Excessive DNA Duplexes Reviewed

Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, Naoki Sugimoto

Chem. Commun 46 5740 - 5742 2010.8

Joint Work

Authorship：Lead author

“生きたバイオマテリアル”としての機能性細胞 Invited Reviewed

長濱宏治

バイオマテリアル 28 193 2010.8

Single Work

Authorship：Lead author

Monomorphic RNA G-quadruplex and polymorphic DNA G-quadruplex structures responding to cellular environmental factors Reviewed

Dong-Hao Zhang, Takeshi Fujimoto, Sarika Saxena, Hai-Qing Yu, Daisuke Miyoshi, and Naoki Sugimoto

Biochemisry 49 4554 - 4563 2010.7

Joint Work

Authorship：Lead author

Preparation of Nanoaggregates through Self-assembly of Amphiphilic Polyrotaxane Composed of PLLA–PEG–PLLA Triblock Copolymer and α-Cyclodextrins Reviewed

Koji Nagahama, Junpei Ohmura, Hiromi Sakaue, Tatsuro Ouchi, Yuichi Ohya and Nobuhiko Yui

Chemistry Letters 39 250 2010.4

Joint Work

Authorship：Lead author

Interleukin 6 enhances glycolysis through expression of the glycolytic enzymes hexokinase 2 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3.

Ando M, Uehara I, Kogure K, Asano Y, Nakajima W, Abe Y, Kawauchi K, Tanaka N

Journal of Nippon Medical School = Nippon Ika Daigaku zasshi 77 ( 2 ) 97 - 105 2010.4

Joint Work

PubMed

Utilization of salmon milt DNA against UV damage Reviewed

Yoshiharu Sasaki, Daisuke Miyoshi, and Naoki Sugimoto

Applied Biochem. Biotech. 160 2458 - 2466 2010.2

Joint Work

Upstream regulatory sequences required for specific gene expression in the ascidian neural tube. Reviewed

Shimai K, Kitaura Y, Tamari Y, Nishikata T

Zoological science 27 ( 2 ) 76 - 83 2010.2

Joint Work

DOI： 10.2108/zsj.27.76

PubMed

The molecular mechanism of glucose metabolism hampered by p53 tumor suppressor protein.

Kawauchi K

Journal of Nippon Medical School = Nippon Ika Daigaku zasshi 77 ( 1 ) 48 - 49 2010.2

Single Work

PubMed

Molecular crowding of the cosolutes induces an intramolecular i-motif structure of triplet repeat DNA oligomers at neutral pH Reviewed

A. Rajendran, S. Nakano, and N. Sugimoto

Chem. Commun. 46 1299 - 1301 2010.1

Joint Work

DOI： 10.1039/B922050J

The upstream regulatory sequences required for specific gene expression in the ascidian neural tube. Reviewed

Shimai K., Kitaura Y., Tamari Y., and Nishikata T.

Zoological Science 27 76 - 83 2010

Joint Work

Fabrication of copper damascene patterns on polyimide using direct metallization of trench templates generated by imprint lithography

T. Matsumura, Y. Enomoto, T. Tsuruoka, K. Akamatsu, H. Nawafune

Langmuir 26 12448 2010

Joint Work

Synthesis of pH-responsive nanocomposite microgels with size-controlled gold nanoparticles from ion-doped, lightly cross-linked poly(vinylpyridine)

K. Akamatsu, M. Shimada, T. Tsuruoka, H. Nawafune, S. Fujii, Y. Nakamura

Langmuir 26 1254 2010

Joint Work

Synthesis and characterization of polypyrrole-palladium nanocomposite-coated latex particles and their use as a catalyst for Suzuki coupling reaction in aqueous media

S. Fujii, S. Matsuzawa, Y. Nakamura, A. Ohtaka, T. Teratani, K. Akamatsu, T, Tsuruoka, H. Nawafune

Langmuir 26 6230 2010

Joint Work

Facile one-step route to polyaniline-silver nanocomposite particles and their application as a colored particulate emulsifier

S. Fujii, Y. Nishimura, A. Aichi, S. Matsuzawa, Y. Nakamura, K. Akamatsu, H. Nawafune

Synthetic Metals 160 1322 2010

Joint Work

Electron Magnetic Resonance in Interacting Ferromagnetic-Metal Nanoparticle Systems: Experiment and Numerical Simulation

C. Mitsumata, S. Tomita, S. Hagiwara, and K. Akamatsu

J. Phys. Cond. Mater. 22 ( 016005 ) 2010

Joint Work

Controlled Multiscale Synthesis of Porous Coordination Polymer in Nano/Micro Regimes

S. Diring, S. Furukawa, Y. Takashima, T. Tsuruoka, S. Kitagawa

Chem. Mater 22 4531 - 4538 2010

Joint Work

「光化学還元法によるガラス基板上への銅のダイレクトパターニング」

有村英俊、中道良太、木村明寛、鶴岡孝章、赤松謙祐、縄舟秀美

エレクトロニクス実装学会誌 13 46 - 51 2010

Single Work

Synthesis of pH-responsive nanocomposite microgels with size-controlled gold nanoparticles from ion-doped, lightly cross-linked poly(vinylpyridine)

K. Akamatsu, M. Shimada, T. Tsuruoka, H. Nawafune, S. Fujii, Y. Nakamura

Langmuir 26 1254 - 1259 2010

Joint Work

Synthesis and characterization of polypyrrole-palladium nanocomposite-coated latex particles and their use as a catalyst for Suzuki coupling reaction in aqueous media

S. Fujii, S. Matsuzawa, Y. Nakamura, A. Ohtaka, T. Teratani, K. Akamatsu, T, Tsuruoka, H. Nawafune

Langmuir 26 6230 - 6230 2010

Joint Work

Facile one-step route to polyaniline-silver nanocomposite particles and their application as a colored particulate emulsifier

S. Fujii, Y. Nishimura, A. Aichi, S. Matsuzawa, Y. Nakamura, K. Akamatsu, H. Nawafune

Synthetic Metals 160 1322 - 1322 2010

Joint Work

Fabrication of copper damascene patterns on polyimide using direct metallization of trench templates generated by imprint lithography

Y. Matsumura, Y. Enomoto, T. Tsuruoka, K. Akamatsu, H. Nawafune

Langmuir 26 12448 - 12454 2010

Joint Work

Electron Magnetic Resonance in Interacting Ferromagnetic-Metal Nanoparticle Systems: Experiment and Numerical Simulation

C. Mitsumata, S. Tomita, S. Hagiwara, and K. Akamatsu

J. Phys. Cond. Mater 22 016005 - 016005 2010

Joint Work

Construction of Detection System for Amyloid beta Peptide Localization and Aggregation Using Fluorescent and Luminescent Fusion Proteins Reviewed

Kenji Usui, Masayasu Mie, Takashi Andou, Naoki Sugimoto, Hisakazu Mihara, Eiry Kobatake

Peptide Science 2009 2009 111 - 112 2010

Joint Work

Authorship：Lead author

Controlled Multiscale Synthesis of Porous Coordination Polymer in Nano/Micro Regimes Reviewed

S. Diring, S. Furukawa, Y. Takashima, T. Tsuruoka, S. Kitagawa

Chem. Mater. 22 4531 - 4538 2010

Joint Work

光化学還元法によるガラス基板上への銅のダイレクトパターニング

有村英俊、中道良太、木村明寛、鶴岡孝章、赤松謙祐、縄舟秀美

エレクトロニクス実装学会誌 13 46 - 51 2010

Joint Work

Synthesis of pH-Responsive Nanocomposite Microgels with Size-Controlled Gold Nanoparticles from Ion-Doped, Lightly Cross-Linked Poly(vinylpyridine) Reviewed

K. Akamatsu, M. Shimada, T. Tsuruoka, H. Nawafune, S. Fujii, Y. Nakamura

Langmuir 26 1254 - 1259 2010

Joint Work

Synthesis and Characterization of Polypyrrole-Palladium Nanocomposite-coated Latex Particles and Their Use as a Catalyst for Suzuki Coupling Reaction in Aqueous Media Reviewed

S. Fujii, S. Matsuzawa, Y. Nakamura, A. Ohtaka, T. Teratani, K. Akamatsu, T. Tsuruoka, H. Nawafune

Langmuir 26 6230 - 6239 2010

Joint Work

Fabrication of Copper Damascene Patterns on Polyimide Using Direct Metallization on Trench Templates Generated by Imprint Lithography Reviewed

Y. Matsumura, Y. Enomoto, T. Tsuruoka, K. Akamatsu, H. Nawafune

Langmuir 26 12448 - 12454 2010

Joint Work

Periodic Molecular Boxes in Entangled Enantiomorphic lcy Nets Reviewed

Yohei Takashima, Charlotte Bonneau, Shuhei Furukawa, Mio Kondo, Ryotaro Matsuda, Susumu Kitagawa

Chem. Commun 46 4142 - 4144 2010

Joint Work

Controlled Multiscale Synthesis of Porous Coordination Polymers in Nano/Micro Regimes Reviewed

St?phane Diring, Shuhei Furukawa, Yohei Takashima, Takaaki Tsuruoka, Susumu Kitagawa

Chem. Mater. 22 4531 - 4538 2010

Joint Work

Control over the Nucleation Process determines the Framework Topology of Porous Coordination Polymers Reviewed

Mio Kondo, Yohei Takashima, Joobeom Seo, Susumu Kitagawa, Shuhei Furukawa

CrystEngComm 12 2350 - 2353 2010

Joint Work

Stabilization of Three-Way Junctions of DNA under Molecular Crowding Conditions Reviewed

Sanjukta Muhuri, Kenta Mimura, Daisuke Miyoshi, and Naoki Sugimoto

J. Am. Chem. Soc. 131 9268 - 9280 2009.12

Joint Work

Neuronal differentiation of neural progenitor cells by intracellular delivery of synthetic oligopeptide derived from von Hippel-Lindau protein Reviewed

H. Kanno, S. Nakano, A. Kubo, T. Mimura, N. Tajima, and N. Sugimoto

Protein & Peptide Letters 16 1291 - 1296 2009.12

Joint Work

DOI： 10.2174/092986609789353691

Dynamics and energetics of the base flipping conformation studied with base pair-mimic nucleosides Reviewed

S. Nakano, H. Oka, Y. Uotani, K. Uenishi, M. Fujii, and N. Sugimoto

Biochemistry 48 11304 - 11311 2009.12

Joint Work

DOI： 10.1021/bi901496q

Face-to-face porphyrin moieties assembled with spacing for pyrazine recognition in molecularly imprinted polymers Reviewed

Matsui, J., Sodeyama, T., Saiki, Y., Miyazawa, T., Yamada, T., Tamaki, K., Murashima, T.

Biosensors and Bioelectronics 25 ( 3 ) 635 - 639 2009.11

Joint Work

Hydration of Watson-Crick base pairs and dehydration of Hoogsteen base pairs inducing structural polymorphism under molecular crowding conditions

三好大輔

J. Am. Chem. Soc. ( 131 ) 3522 - 3531 2009.11

Single Work

Site-specific modification of Alzheimer's peptides by cholesterol oxidation products enhances aggregation energetics and neurotoxicity Reviewed International coauthorship

Kenji Usui, John D. Hulleman, Johan F. Paulsson, Sarah J. Siegel, Evan T. Powers, Jeffery W. Kelly

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 106 ( 44 ) 18563 - 18568 2009.11

Publisher：NATL ACAD SCIENCES

Accumulation of amyloid beta-peptide (A beta) and tau aggregates, possibly linked to age-associated deficiencies in protein homeostasis, appear to cause Alzheimer's disease. Schiff-base formation between A beta and the aldehyde-bearing cholesterol oxidation product 3-beta-hydroxy-5-oxo-5,6-secocholestan-6-al is known to increase A beta amyloidogenicity. Here, we synthesized A beta variants site-specifically modified with the cholesterol aldehyde at Asp-1, Lys-16, or Lys-28, rather than studying mixtures. These distinct modifications have a similar effect on the thermodynamic propensity for aggregation, enabling aggregation at low concentrations. In contrast, the modification site differentially influences the aggregation kinetics; Lys-16-modified A beta formed amorphous aggregates fastest and at the lowest concentration (within 2 h at a concentration of 20 nM), followed by the Lys-28 and Asp-1 conjugates. Also, the aggregates resulting from A beta Lys-16 cholesterol aldehyde conjugation were more toxic to primary rat cortical neurons than treatment with unmodified A beta under identical conditions and at the same concentration. Our results show that A beta modification by cholesterol derivatives, especially at Lys-16, renders it kinetically and thermodynamically competent to form neurotoxic aggregates at concentrations approaching the physiologic concentration of A beta.

DOI： 10.1073/pnas.0804758106

PubMed

Facilitation of RNA enzyme activity in the molecular crowding media of cosolutes Reviewed

S. Nakano, H. T. Karimata, Y. Kitagawa, and N. Sugimoto

J. Am. Chem. Soc. 131 16881 - 16888 2009.10

Joint Work

DOI： 10.1021/ja9066628

Biodegradable poly(l-lactide)-grafted α-cyclodextrin copolymer displaying specific dye absorption by host-guest interact Reviewed

Koji Nagahama, Keiko Shimizu, Tatsuro Ouchi, Yuichi Ohya

Reactive and Functional Polymers 69 891 2009.10

Joint Work

Authorship：Lead author

Thermo-Sensitive Sol-Gel Transition of Poly(depsipeptide-co-lactide)-g-PEG Copolymers in Aqueous Solution Reviewed

Koji Nagahama, Yuichiro Imai, Teppei Nakayama, Junpei Ohmura, Tatsuro Ouchi, Yuichi Ohya

Polymer 50 3547 2009.10

Joint Work

Authorship：Lead author

Polyrotaxane Composed of Poly-L-Lactide and α-Cyclodextrin Exhibiting Protease-Triggered Hydrolysis Reviewed

Yuichi Ohya, Seigo Takamido, Koji Nagahama, Tatsuro Ouchi, Ryo Katoono, Nobuhiko Yui

Biomacromolecules 10 2261 2009.10

Joint Work

Accurate curve fitting procedure for UV melting analysis of highly thermostable RNA hairpins（共著）

J. Kawakami, Y. Tanaka, and K. Kishimoto

Nucleic Acids Symp. Ser. 53 227 - 228 2009.9

Joint Work

Authorship：Lead author

DOI： 10.1093/nass/nrp114

Thermal stability and hydration state of DNA G-quadruplex regulated by loop regions

T. Fujimoto, D. Miyoshi, H. Tateishi-Karimata, and N. Sugimoto

Nucleic Acids Symp Ser (Oxf) 53 237 - 238 2009.9

Joint Work

Authorship：Lead author

DNA junction structure stabilized by molecular crowding conditions

D. Miyoshi, S. Muhuri, K. Mimura, and N. Sugimoto

Nucleic Acids Symp Ser (Oxf) 53 59 - 60 2009.9

Joint Work

Authorship：Lead author

DNA junction structure stabilized by molecular crowding conditions

D. Miyoshi, S. Muhuri, K. Mimura, N. Sugimoto

Nucleic Acids Symp Ser (Oxf) 53 59 - 60 2009.9

Joint Work

Investigations of the cation binding to nucleotides by monitoring the hairpin-duplex equilibrium of a self-complementary sequence

S. Nakano, H. Hirayama, and N. Sugimoto

Nucleic Acids Symp. Ser. 53 229 - 230 2009.9

Joint Work

Porous Coordination Polymer with Pyridinium Cationic Surface, [Zn-2(tpa)(2)(Cpb)]

Higuchi Masakazu, Tanaka Daisuke, Horike Satoshi, Sakamoto Hirotoshi, Nakamura Kohei, Takashima Yohei, Hijikata Yuh, Yanai Nobuhiro, Kim Jungeun, Kato Kenichi, Kubota Yoshiki, Takata Masaki, Kitagawa Susumu

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 131 ( 30 ) 10336 - + 2009.8

Joint Work

DOI： 10.1021/ja900373v

Hydrophobically Modified Biodegradable Poly(ethylene glycol) Copolymers that Form Temperature-Responsive Nanogels Reviewed

Koji Nagahama, Mihoko Hashizume, Hidetoshi Yamamoto, Tatsuro Ouchi, Yuichi Ohya

Langmuir 25 9734 2009.8

Joint Work

Authorship：Lead author

Biodegradable Shape-Memory Polymers Exhibiting Sharp Thermal Transitions and Controlled Drug Release Reviewed

Koji Nagahama, Yuichi Ueda, Tatsuro Ouchi, Yuichi Ohya

Biomacromolecules 10 1789 2009.8

Joint Work

Authorship：Lead author

“Geometric and electronic structure differences between the type 3 copper sites of the multicopper oxidases and hemocyanin/tyrosinase” Reviewed

Jungjoo Yoon, Satoshi Fujii, Edward I. Solomon

Proc. Natl. Acad. Sci. USA 106 ( 16 ) 6585 - 6590 2009.4

Joint Work

Reversible stability switching of a hairpin DNA via a photo-responsive linker unit Invited Reviewed

Li Wu, Kazuya Koumoto, Naoki Sugimoto

Chemical Communications 1915 - 1917 2009.4

Joint Work

ヘアピンDNAのループ部位に導入し、光異性化によってヘアピンDNA全体の安定性を制御できる新規光応答性ユニットの開発に成功した。このユニットをヘアピン部位に導入したDNAは紫外光、可視光の照射に伴って安定性が可逆的に制御することができた。

Nuclease Resistant Methylphosphonate-DNA/LNA Chimeric Oligonucleotides Reviewed

Koji Nagahama, Rakesh Veedu, Jesper Wengel

Bioorganic and Medicinal Chemistry Letters 19 2707 2009.3

Joint Work

Authorship：Lead author

Loss of p53 enhances catalytic activity of IKKbeta through O-linked beta-N-acetyl glucosamine modification.

Kawauchi K, Araki K, Tobiume K, Tanaka N

Proceedings of the National Academy of Sciences of the United States of America 106 ( 9 ) 3431 - 3436 2009.3

Joint Work

DOI： 10.1073/pnas.0813210106

PubMed

Effects of Polydepsipeptide Side-Chain Groups on the Temperature Sensitivity of Triblock Copolymers Composed of Polydepsipeptides and Poly(ethylene glycol) Reviewed

Yuichi Ohya, Hidetoshi Yamamoto, Koji Nagahama, Tatsuro Ouchi

Journal of Polymer Science, Part A: Polymer Chemistry 47 3892 2009.2

Joint Work

DNAは細胞内で三重らせん・四重らせん構造を形成しているのか？ Invited

三好大輔、杉本直己

化学と生物 7 449 - 451 2009.1

Joint Work

Cu(II) ion inhibits early stages of amyloid β fibril formation in vitro

T. Fujimoto, D. Miyoshi, H. Tateishi-Karimata, and N. Sugimoto

J. Biol. Inorg. Chem., Suppl. 1 S118 2009.1

Joint Work

An Approach to Peptide-based ATP Receptors by a Combination of Random Selection, Rational Design, and Molecular Imprinting Reviewed

J. Matsui, J. Nagano, D. Miyoshi, K. Tamaki, N. Sugimoto

Biosens. Bioelectron. 25 563 - 567 2009.1

Joint Work

Cu(II) ion inhibits early stages of amyloid β fibril formation in vitro

T. Fujimoto, D. Miyoshi, H. Tateishi-Karimata, N. Sugimoto

J. Biol. Inorg. Chem., Suppl. 1 S118 2009.1

Joint Work

DNAは細胞内で三重らせん・四重らせん構造を形成しているのか？

三好大輔, 杉本直己

化学と生物 7 449 - 451 2009.1

Joint Work

Molecularly Imprinted Nanocomposites for Highly Sensitive SPR Detection of a Non-aqueous Atrazine Sample　 Reviewed

J. Matsui, M. Takayose, K. Akamatsu, H. Nawafune, K. Tamaki, N. Sugimoto

Analyst 2009

Joint Work

Authorship：Lead author

Face-to-face Porphyrin Moieties Assembled with Spacing for Pyrazine Recognition in Molecularly Imprinted Polymers Reviewed

J. Matsui, T. Sodeyama, Y. Saiki, T. Miyazawa, T. Yamada, K. Tamaki, T. Murashima

Biosensors and Bioelectronics 2009

Joint Work

Authorship：Lead author

An Approach to Peptide-based ATP Receptors by a Combination of Random Selection, Rational Design, and Molecular Imprinting Reviewed

J. Matsui, J. Nagano, D. Miyoshi, K. Tamaki, N. Sugimoto

Biosensors and Bioelectronics 2009

Joint Work

Authorship：Lead author

Interaction between Oocyte and Accessory Cells during Ascidian Oogenesis.

Shimai K., Ishii H., and Nishikata T.

Mem. Konan Univ., Sci. Eng. Ser. 56 31 - 41 2009

Joint Work

ベンゾトリアゾール誘導体のマイクロコンタクトプリンティングを利用するULSI銅微細配線の形成

有村英俊、尾山祐斗、鶴岡孝章、赤松謙祐、縄舟秀美

表面技術 60 598 - 602 2009

Joint Work

Enhancement of photoluminescence from silicon nanocrystals by metal nanostructures made by nanosphere lithography

Y. Mochizuki, M. Fujii, S. Hayashi, T. Tsuruoka, K. Akamatsu

J. Appl. Phys. 106 013517 2009

Joint Work

Site-selective direct nickel metallization on precursor polyimide using microcontact printing

Y. Matsumura, Y. Enomoto, K. Akamatsu, H. Nawafune

J. Surf, Finish, Soc. Jpn. 60 61 - 62 2009

Joint Work

Molecularly imprinted nanocomposites for highly sensitive SPR detection of an non-aqueous atrazine sample

J. Matui, M. Takayose, K. Akamatsu, H. Nawafune, K. Tamaki, K. Sugimoto

Analyst 134 80 - 86 2009

Joint Work

A designed peptide chip: Protein fingerprinting technology with a dry peptide array and statistical data mining Reviewed

Kenji Usui, Kin-Ya Tomizaki, Hisakazu Mihara

Methods in Molecular Biology 570 273 - 284 2009

There has recently been increased interest in the potential for microarray technologies to study protein networks in a whole cell system within a single experiment. Protein-detecting microarrays are composed of numerous agents immobilized within a tiny area on solid surfaces to capture targeted proteins and to detect interactions in a high-throughput fashion. In this chapter, in order to extend the usability of peptide microarrays, we describe a novel dry peptide microarray format to obtain protein fingerprint (PFP) data sets and a statistical PFP data manipulation technique to quantitatively analyze targeted proteins. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.

DOI： 10.1007/978-1-60327-394-7_13

PubMed

Nanoporous Nanorods Fabricated by Coordination Modulation and Oriented Attachment Growth Reviewed

T. Tsuruoka, S. Furukawa, Y. Takashima, K. Yoshida, S. Isoda, S. Kitagawa

Angew. Chem. Int. Ed. 48 4739 - 4743 2009

Joint Work

印刷中

Heterogeneously Hybridized Porous Coordination Polymer Crystals: Fabrication of Heterometallic Core–Shell Single Crystals with an In-Plane Rotational Epitaxial Relationship Reviewed

S. Furukawa, K. Hirai, K. Nakagawa, Y. Takashima, R. Matsuda, T. Tsuruoka, M. Kondo, R. Haruki, D. Tanaka, H. Sakamoto, S. Shimomura, O. Sakata, S. Kitagawa

Angew. Chem. Int. Ed. 48 1766 - 1770 2009

Joint Work

A Block PCP Crystal: Anisotropic Hybridization of Porous Coordination Polymers by Face-Selective Epitaxial Growth Reviewed

S. Furukawa, K. Hirai, Y. Takashima, K. Nakagawa, M. Kondo, T. Tsuruoka, O. Sakata, S. Kitagawa

Chem. Commun. 5097 - 5099 2009

Joint Work

ベンゾトリアゾール誘導体のマイクロコンタクトプリンティングを利用するULSI銅微細配線の形成 Reviewed

有村英俊、尾山祐斗、鶴岡孝章、赤松謙祐、縄舟秀美

表面技術 60 598 - 602 2009

Joint Work

Enhancement of Photoluminescence from silicon nanocrystals by metal nanostructures made by nanosphere lithography Reviewed

Y. Mochizuki, M. Fujii, S. Hayashi, T. Tsuruoka, K. Akamatsu

J. Appl. Phys. 106 013517 2009

Joint Work

Heterogeneously Hybridized Porous Coordination Polymer Crystals: Fabrication of Heterometallic Core-Shell Single Crystals with an In-Plane Rotational Epitaxial Relationship Reviewed

Shuhei Furukawa, Kenji Hirai, Keiji Nakagawa, Yohei Takashima, Ryotaro Matsuda, Takaaki Tsuruoka, Mio Kondo, Rie Haruki, Daisuke Tanaka, Hirotoshi Sakamoto, Satoru Shimomura, Osami Sakata and Susumu Kitagawa

Angew. Chem. Int. Ed 48 1766 - 1770 2009

Joint Work

A Block PCP Crystal: Anisotropic Hybridization of Porous Coordination Polymers by Face-Selective Epitaxial Growth Reviewed

Shuhei Furukawa, Kenji Hirai, Yohei Takashima, Keiji Nakagawa, Mio Kondo, Takaaki Tsuruoka, Osami Sakata, Susumu Kitagawa

Chem. Commun 5097 - 5099 2009

Joint Work

Porous Coordination Polymer with Pyridinium Cationic Surface, [Zn2(tpa)2(cpb)] Reviewed

Masakazu Higuchi, Daisuke Tanaka, Satoshi Horike, Hirotoshi Sakamoto, Kohei Nakamura, Yohei Takashima, Yuh Hijikata, Nobuhiro Yanai, Jungeun Kim, Kenichi Kato, Yoshiki Kubota, Masaki Takata, Susumu Kitagawa

J. Am. Chem. Soc 131 10336 - 10337 2009

Joint Work

Nanoporous Nanorods Fabricated by Coordination Modulation and Oriented Attachment Growth Reviewed

Takaaki Tsuruoka, Shuhei Furukawa, Yohei Takashima, Kaname Yoshida, Seiji Isoda and Susumu Kitagawa

Angew. Chem. Int. Ed 48 4739 - 4743 2009

Joint Work

Design of allele-specific primers and detection of the human ABO genotyping to avoid the pseudopositive problem Reviewed

H. Yaku, T. Yukimasa, S. Nakano, N. Sugimoto, and H. Oka

Electrophoresis, 29 4130 - 4140 2008.12

Joint Work

DOI： 10.1002/elps.200800097

Structural effect of synthetic zwitterionic cosolutes on the stability of DNA duplexes Reviewed

甲元一也

Tetrahedron ( 64 ) 168 - 174 2008.11

Joint Work

Authorship：Lead author

Small-molecule-directed assembly of DNA: a gold-nanoparticle-based strategy for screening of a DNA duplex binders

三好大輔

Adv. Mat ( 20 ) 706 - 710 2008.11

Single Work

Telomerase activity regulated by thermodynamic stability of DNA・RNA hybrid

三好大輔

Angew. Chem. Int. Ed., ( 47 ) 9034 - 9038 2008.11

Single Work

Temperature-Induced Hydrogel through Self-Assembly of Cholesterol-substituted Star PEG-b-PLLA Copolymers as an Injectable Scaffold for Tissue Engineering Reviewed

K. Nagahama, T. Ouchi, Y. Ohya,

Adv. Funct. Mater. 18 ( 8 ) 1220 - 1231 2008.11

Joint Work

Authorship：Lead author

Biodegradable Nanogels Prepared by Self-Assembly of Poly(L-Lactide)-Grafted Dextran: Entrapment and Release of Protein Reviewed

K. Nagahama, T. Ouchi, Y. Ohya,

Macromol. Biosci. 8 ( 11 ) 1044 - 1952 2008.11

Joint Work

Authorship：Lead author

Irreversible Temperature-Responsive Formation of High-strength Hydrogel from an Enantiomeric Mixture of Starburst Triblock Copolymers Consisting of 8-arm PEG and PLLA or PDLA Reviewed

K. Nagahama, K. Fujiura, S. Enami, T. Ouchi, Y. Ohya,

J. Polym. Sci. Part A Polym. Chem., 46 ( 18 ) 6317 - 6332 2008.11

Joint Work

Resolution of secondary alcohols via Carica papaya lipase-catalyzed enantioselective acylation Reviewed

Miyazawa, T., Houhashi, M., Inoue, Y., Murashima, T., Yamada, T.

Biotechnology Letters 30 ( 10 ) 1783 - 1787 2008.10

Joint Work

Peptide synthesis using carbamoylmethyl esters as acyl donors mediated by Bacillus amyloliquefaciens protease Reviewed

Miyazawa, T., Shindo, T., Murashima, T., Yamada, T.

Protein and Peptide Letters 15 ( 10 ) 1050 - 1054 2008.10

Joint Work

Molecular crowding effect on metal ion binding properties of the hammerhead ribozyme

S. Nakano, Y. Kitagawa, H. T. Karimata, and N. Sugimoto

Nucleic Acids Symp. Ser. 52 519 - 520 2008.9

Joint Work

IKK/NF-kappaB signaling pathway inhibits cell-cycle progression by a novel Rb-independent suppression system for E2F transcription factors. Reviewed

Araki K, Kawauchi K, Tanaka N

Oncogene 27 ( 43 ) 5696 - 5705 2008.9

Joint Work

DOI： 10.1038/onc.2008.184

PubMed

Activated p53 induces NF-kappaB DNA binding but suppresses its transcriptional activation.

Kawauchi K, Araki K, Tobiume K, Tanaka N

Biochemical and biophysical research communications 372 ( 1 ) 137 - 141 2008.7

Joint Work

DOI： 10.1016/j.bbrc.2008.05.021

PubMed

Conformation and the sodium ion condensation on DNA and RNA structures in the presence of a neutral cosloute as a mimic of the intracellular media Reviewed

Shuichi Nakano, Lei Wu, Hirohito Oka, Hisae Tateishi-Karimata, Toshimasa Kirihata, Yuichi Sato, Satoshi Fujii, Hiroshi Sakai, Masayuki Kuwahara, Hiroaki Sawai, and Naoki Sugimoto

Mol. BioSys. 4 579 - 588 2008.6

Joint Work

Cycloprodigiosin hydrochloride activates the Ras-PI3K-Akt pathway and suppresses protein synthesis inhibition-induced apoptosis in PC12 cells.

Kawauchi K, Tobiume K, Iwashita K, Inagaki H, Morikawa T, Shibukawa Y, Moriyama Y, Hirata H, Kamata H

Bioscience, biotechnology, and biochemistry 72 ( 6 ) 1564 - 1570 2008.6

Joint Work

DOI： 10.1271/bbb.80064

PubMed

Cationic Porphyrin Induced a Telomeric DNA to G-Quadruplex Form in Water. Reviewed

Murashima, T., Sakiyama, D., Miyoshi, D., Kuriyama, M., Yamada, T., Miyazawa, T., Sugimoto, N.

Bioinorganic Chemistry and Applications 2008.5

Joint Work

Authorship：Lead author

Bioinorg. Chem. Appl. （学術雑誌、2008） 2008, Article ID 294756,

p53 regulates glucose metabolism through an IKK-NF-kappaB pathway and inhibits cell transformation.

Kawauchi K, Araki K, Tobiume K, Tanaka N

Nature cell biology 10 ( 5 ) 611 - 618 2008.5

Joint Work

DOI： 10.1038/ncb1724

PubMed

“Reaction of iron(III)-polyaminocarboxylate complexes with hydrogen peroxide: Correlation between ligand structure and reactivity.” Reviewed

Satoshi Fujii, Chiyo Tsueda, Kunio Yamabe, Kohsuke Nakajima, and Hiroshi Sakai

Inorg. Chim. Acta 361 1207 - 1211 2008.3

Joint Work

Design of Polylactide-Based Hydrogels as Biodegradable Soft-Biomaterials

Koji NAGAHAMA

2008.3

Single Work

Hedgehog signaling overrides p53-mediated tumor suppression by activating Mdm2.

Abe Y, Oda-Sato E, Tobiume K, Kawauchi K, Taya Y, Okamoto K, Oren M, Tanaka N

Proceedings of the National Academy of Sciences of the United States of America 105 ( 12 ) 4838 - 4843 2008.3

Joint Work

DOI： 10.1073/pnas.0712216105

PubMed

Capture of cationic ligands bound diffusely to base pairs during the DNA refolding

S. Nakano, T. Kirihata, and N. Sugimoto

Chem. Commun. ( 6 ) 700 - 702 2008.2

Joint Work

DOI： 10.1039/b715647b

CiNii Article

Conformation and the sodium ion condensation on DNA and RNA structures in the presence of a neutral cosolute as a mimic of the intracellular media Invited Reviewed

S. Nakano, L. Wu, H. Oka, H. T. Karimata, T. Kirihata, Y. Sato, S. Fujii, H. Sakai, M. Kuwahara, H. Sawai, and N. Sugimoto

Mol. BioSyst. 4 579 - 588 2008.2

Joint Work

DOI： 10.1039/B718806D

Recognition of a flipped base in a hairpinloop DNA by a small peptide （共著） Reviewed

J. Kawakami, S. Okabe, Y. Tanabe, and N. Sugimoto

Nucleoside, Nucleotides and Nucleic Acids 27 292 - 308 2008.2

Joint Work

Authorship：Lead author

DOI： 10.1080/15257770701845261

Riboswitches for enhancing target gene expression in eukaryotes Reviewed

T. Yamauchi, D. Miyoshi, T. Kubodera, M. Ban, A. Nishimura, and N. Sugimoto

ChemBioChem 9 1040 - 1043 2008.1

Joint Work

外部刺激に応答する機能性核酸－核酸ならではの機能を活用したナノバイオマテリアルの設計－ Invited

三好大輔、杉本直己

化学工業　（化学工業社） 59 ( 11 ) 21 - 27 2008.1

Joint Work

Thermodynamics of DNA structures under molecular crowding conditions with neutral and positive charged cosolutes

D. Miyoshi, K. Nakamura, S. Muhuri, H. T. Karimata, N. Sugimoto

Nucleic Acids Symp. Ser. 52 413 - 414 2008.1

Joint Work

Cationic porphyrin induced a telomeric DNA to g-quadruplex form in water Reviewed

T. Murashima, D. Sakiyama, D. Miyoshi, M. Kuriyama, T. Yamada, T. Miyazawa, N. Sugimoto

Bioinorg Chem Appl. 294756 2008.1

Joint Work

Molecular crowding effects on structure and stability of DNA Invited Reviewed

D. Miyoshi and N. Sugimoto

Biochimie 90 1040 - 1051 2008.1

Joint Work

外部刺激に応答する機能性核酸－核酸ならではの機能を活用したナノバイオマテリアルの設計－

三好大輔, 杉本直己

化学工業（化学工業社） 59 ( 11 ) 21 - 27 2008.1

Joint Work

Highly regioselective propanoylation of dihydroxybenzenes mediated by Candida antarctica lipase B in organic solvents Reviewed

Miyazawa, T., Hamada, M., Morimoto, R., Murashima, T., Yamada, T.

Tetrahedron Letters 49 ( 1 ) 175 - 178 2008.1

Joint Work

“Spectroscopic Studies of Perturbed T1 Cu Sites in the Multicopper Oxidases Saccharomyces cerevisiae Fet3p and Rhus vernicifera Laccase: Allosteric Coupling between the T1 and Trinuclear Cu Sites” Reviewed

Anthony J. Augustine, Mads Emil Kragh, Ritimukta Sarangi, Satoshi Fujii, Barry D. Liboiron, Christopher S. Stoj, Daniel J. Kosman, Keith O. Hodgson, Britt Hedman, and Edward I. Solomon

Biochemistry 47 ( 7 ) 2036 - 2045 2008.1

Joint Work

Preparation and Characterization of Au Nanoparticle/Polymer composite Photonic Crystals　

T. Tsuruoka, S. Samitsu, T. Aokata, J. Matsui, T. Murashima, K. Akamatsu, H. Nawafune

Trans.MRS-Japan 717 - 720 2008

Joint Work

Novel ubiquitous promoters and expression-vector optimization in ascidian embryos.

西方敬人

Invertebrate Reproduction & Development ( 51 ) 103 - 110 2008

Single Work

Multimodal pathways during UVB-induced apoptosis in HL-60.

西方敬人

Proceedings of the 14th Biennial Congress of the International Society for Free Radical Research, Beijing, China 2008

Single Work

Nanocomposite polymeric microspheres containing Ni nanoparticles with controlled microstructures

K. Akamatsu, S. Adachi, T. Tsuruoka, S. Ikeda, S. Tomita, H. Nawafune

Chem. Mater. 20 3042 - 3047 2008

Joint Work

Immobilization and Assembly of Mixed Monolayer-Protected Au Nanoparticles on Glass substrate via Covalent Bond Interconnection

T. Tsuruoka, A. Fujimoto, K. Akamatsu, H. Nawafune

Trans. Mater. Res. Soc. Jpn. 33 157 - 160 2008

Joint Work

Highly luminescent mono- and multilayers of immobilized CdTe nanocrystals: controlling optical properties through post chemical surface modification

T. Tsuruoka, R. Takahashi, T. Nakamura, M. Fujii, K. Akamatsu, and H. Nawafune

Chem. Commun. 1641 - 1643 2008

Joint Work

Control of the optical properties of CdTe nanocrystals by selective exchange of Te with thiolate: effect of organic ligands on the formation of core-shell structures

T. Tsuruoka, R. Takahashi, K. Akamatsu, H. Nawafune

Phys. Chem. Chem. Phys. 10 2221 - 2226 2008

Joint Work

Direct metallization of nickel on polymeric template patterns for fabrication of copper circuits on glass substrates

Y. Matsumura, Y. Enomoto, M. Sugiyama, K. Akamatsu, H. Nawafune

J. Mater. Chem. 18 5058 - 5082 2008

Joint Work

Fabrication of conductive thin films on precursor polyimide via chemical reduction of nickel (II) complex

Y. Matsumura, Y. Enomoto, T. Jyo, K. Akamatsu, H. Nawafune

J. Surf, Finish, Soc. Jpn. 59 562 - 564 2008

Joint Work

Formation of metal thin film nanocomposite layer from precursor polyimide containing metal complex

M. Matsumura, Y. Enomoto, K. Akamatsu, H. Nawafune

Trans. Mater. Res. Soc. Jpn. 33 1333 - 1336 2008

Joint Work

Molecular crowding effect on metal ion binding properties of the hammerhead ribozyme

S. Nakano, Y. Kitagawa, H. T. Karimata, and N. Sugimoto

Nucleic Acids Symp. Ser. 52 519 - 520 2008

Joint Work

Nanocomposite Polymeric Microspheres Containing Ni Nanoparticles with Controlled Microstructures Reviewed

K. Akamatsu, S. Adachi, T. Tsuruoka, S. Ikeda, S. Tomita, H. Nawafune

Chem. Mater. 20 3042 - 3047 2008

Joint Work

Highly Luminescent Mono- and Multilayers of Immobilized CdTe Nanocrystals: Controlling Optical Properties Through Post Chemical Surface Modification Reviewed

T. Tsuruoka, R. Takahashi, T. Nakamura, M. Fujii, K. Akamatsu, H. Nawafune

Chem. Commun. 1641 - 1643 2008

Joint Work

Control of the Optical Properties of CdTe Nanocrystals by Selective Exchange of Te with Thiolate: Effect of Organic Ligands on the Formation of Core-Shell Structures Reviewed

T. Tsuruoka, R. Takahashi, K. Akamatsu, H. Nawafune

Phys. Chem. Chem. Phys. 10 2221 - 2226 2008

Joint Work

Immobilization and Assembly of Mixed Monolayer-Protected Au Nanoparticles on Glass Substrate via Covalent Bond Interconnection Reviewed

T. Tsuruoka, A. Fujimoto, K. Akamatsu, H. Nawafune

Trans. Mater. Res. Soc. Jpn. 33 157 - 160 2008

Joint Work

Preparation and Characterization of Au Nanoparticles/Polymer Composite Photonic Crystals Reviewed

T. Tsuruoka, S. Samitsu, T. Aokata, J. Matui, T. Murashima, K. Akamatsu, H. Nawafune

Trans. Mater. Res. Soc. Jpn. 33 717 - 720 2008

Joint Work

Influence of cationic molecules on the hairpin to duplex equilibria of self-complementary DNA and RNA oligonucleotides Reviewed

S. Nakano, T. Kirihata, S. Fujii, H. Sakai, M. Kuwahara, H. Sawai, and N. Sugimoto

Nucleic Acids Research 35 486 - 494 2007.12

Joint Work

DOI： 10.1093/nar/gkl1073

Synthesis, structure and thermal stability of fully hydrophobic porphyrin-DNA conjugates.

Murashima, T., Hayata, K., Saiki, Y., Matsui, J., Miyoshi, D., Yamada, T., Miyazawa, T., Sugimoto, N.

Tetrahedron Lett., 48 ( 48 ) 8514 - 8517 2007.11

Joint Work

Authorship：Lead author

Tetrahedron Lett., 　（学術雑誌、2007）48, 8514-8517.

Secondary alcohols act as better nucleophiles than primary alcohols in the lipase-catalyzed regioselective deacylation of dihydroxybenzenes acylated at both phenolic hydroxyls.

Miyazawa, T., Hamada, M., Morimoto, R., Murashima, T., Yamada, T.

Tetrahedron Lett., 48 ( 47 ) 8334 - 8337 2007.11

Joint Work

Tetrahedron Lett., （学術雑誌、2007）48, 8334-8337.

Artificial G-wire Switch with 2,2’-Bipyridine Units Responsive to Divalent Metal Ions

甲元一也

J. Am. Chem. Soc ( 129 ) 5919 - 5925 2007.11

Single Work

５）天然高分子　核酸エネルギーデータベースに基づく核酸医薬の設計

甲元一也

絵で見てわかるナノDDS　遺伝子医学MOOK別冊（メディカルドゥ） 63 - 69 2007.11

Single Work

Conformational switch of a functional nanowire based on the DNA G-quadruplex

甲元一也

Nucleic Acids Symp. Ser. ( 51 ) 251 - 252 2007.11

Single Work

Delivery of antisense oligonucleotides to nuclear telomere RNA by use of a complex between polysaccharide and polynucleotide

甲元一也

Bull. Chem. Soc. Jpn ( 80 ) 1091 - 1098 2007.11

Single Work

Molecular Imprinting Under Molecular Crowding Conditions: an Aid to the synthesis of a High-capacity Polymeric Sorbent for Triazine Herbicides

三好大輔

Anal. Chem ( 79 ) 1749 - 1757 2007.11

Single Work

DNAを用いたバイオ計測　－センシング核酸の開発と新展開－

三好大輔

未来材料, 7 ( 6 ) 10 - 18 2007.11

Single Work

Regulation of DNA nucleases by molecular crowding

三好大輔

Nucleic Acids Res ( 35 ) 4086 - 4093 2007.11

Single Work

Artificial G-wire Switch with 2,2’-Bipyridine Units Responsive to Divalent Metal Ions

三好大輔

J. Am. Chem. Soc ( 129 ) 5919 - 5925 2007.11

Single Work

DNAデザイン－機能性分子開発への道しるべ－

三好大輔

化学 62 ( 9 ) 28 - 32 2007.11

Single Work

DNA四重らせん構造を細胞内環境因子で制御する

三好大輔

生体機能関連化学部会 News Letter, 22 ( 3 ) 14 - 17 2007.11

Single Work

Synthesis, structure and thermal stability of fully hydrophobic porphyrin-DNA conjugates

三好大輔

Tetrahedron Lett ( 48 ) 8514 - 8517 2007.11

Single Work

Effects of cosolutes on the thermodynamic stability of parallel DNA duplex and triplex

三好大輔

Nucleic Acids Symp. Ser. (Oxf), ( 51 ) 167 - 168 2007.11

Single Work

Conformational switch of a functional nanowire based on the DNA G-quadruplex

三好大輔

Nucleic Acids Symp. Ser. (Oxf), ( 51 ) 167 - 168 2007.11

Single Work

DNA四重らせん構造を細胞内環境因子で制御する

三好大輔

生体機能関連化学部会 News Letter, 22 ( 3 ) 14 - 17 2007.11

Single Work

Effects of polyethylene glycol on a DNA duplex stability differed by the NaCl concentration Reviewed

H. Karimata, S. Nakano, and N. Sugimoto

Bull. Chem. Soc. Jpn. 80 1987 - 1994 2007.11

Joint Work

The base-pairing ability of the base pair-mimic nucleosides

S. Nakano, K. Uenishi, M. Fujii, and N. Sugimoto

Nucleic Acids Symp. Ser. 51 71 - 72 2007.11

Single Work

Impacts of Stereoregularity and Stereocomplex Formation on Physicochemical, Protein Adsorption and Cell Adhesion Behaviors of Star-Shaped 8-Arms Poly(ethylene glycol)-Poly(lactide) Block Copolymer Films Reviewed

K. Nagahama, Y. Nishimura, Y. Ohya,

Polymer, 48 ( 9 ) 2649 - 2658 2007.11

Joint Work

Authorship：Lead author

Exhibition of Soft and Tenacious Characters Based on Liquid Crystal Formation by Introduction of Cholesterol Groups on Biodegradable Lactide Copolymer Reviewed

K. Nagahama, Y. Ueda, T. Ouchi, Y. Ohya

Biomacromolecules, 8 ( 12 ) 3938 - 3943 2007.11

Joint Work

Authorship：Lead author

Molecular “Screw and Nut”: α-Cyclodextrin Recognizes Polylactide Chirality Reviewed

Y. Ohya, S. Takamido, K. Nagahama, T. Ouchi, T. Ooya, R. Katoono, N. Yui

Macromolecules, 40 ( 18 ) 6441 - 6444 2007.11

Joint Work

Biodegradable Nanogel Formation of Polylactide-Grafted Dextran Copolymer in Dilute Aqueous Solution and Enhancement of Its Stability by Stereocomplexation, Reviewed

K. Nagahama, Y. Mori Y. Ohya, T. Ouchi

Biomacromolecules 8 ( 7 ) 2135 - 2141 2007.11

Joint Work

Authorship：Lead author

Functionalization of DNA by the base pair-mimic nucleosides

H. Oka, S. Nakano, Y. Uotani, K. Uenishi, M. Fujii, and N. Sugimoto

Nucleic Acids Symp. Ser. 51 151 - 152 2007.11

Joint Work

Suppression of AP-1 activity by cycloprodigiosin hydrochloride.

Kawauchi K, Tobiume K, Kaneko S, Kaneshiro K, Okamoto S, Ueda E, Kamata H, Moriyama Y, Hirata H

Biological & pharmaceutical bulletin 30 ( 9 ) 1792 - 1795 2007.9

Joint Work

PubMed

Schizophyllan, beta-1,3-glucan,-oligoamine conjugates as non-viral vector Reviewed

Takeshi Nagasaki, Atsushi Uno, Kazuya Koumoto, Kazuo Sakurai, Seiji Shinkai

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 234 2007.8

Publisher：AMER CHEMICAL SOC

Consecutive GC base pairs determine the energy barrier of DNA duplex formation under molecularly crowded conditions Reviewed

X. Gu, S. Nakano, and N. Sugimoto

Chem. Commun. 2750 - 2752 2007.4

Joint Work

DOI： 10.1039/B702865B

Mechanistic characterization of the HDV genomic ribozyme: A mutant of the C41 motif provides insight into positioning and thermodynamic linkage of metal ions and protons Reviewed

S. Nakano and P. C. Bevilacqua

Biochemistry 46 3001 - 3012 2007.2

Joint Work

DOI： 10.1021/bi061732s

Molecular Imprinting Under Molecular Crowding Conditions: an Aid to the synthesis of a High-capacity Polymeric Sorbent for Triazine Herbicides.

Matsui, J., Goji, S., Murashima, T., Miyoshi, D., Komai, S., Shigeyasu, A., Kushida, T

Anal. Chem., 79 ( 4 ) 1749 - 1757 2007.2

Joint Work

Anal. Chem., （学術雑誌、2007）79, 1749-1757.

Hydration regulates thermodynamic stability of DNA structures under molecular crowding conditions Reviewed

D. Miyoshi, H. Karimata, and N. Sugimoto

Nucleosides, Nucleotides and Nucleic Acids 26 589 - 595 2007.1

Joint Work

“Modulation of the DNA and RNA Secondary Structure by Cationic Molecule” Reviewed

Shu-ichi Nakano, Toshimasa Kirihata, Satoshi Fujii, Hiroshi Sakai, Masayuki Kuwahara, Hiroaki Sawai, and Naoki Sugimoto

Nucleic Acids Res 35 ( 2 ) 486 - 494 2007.1

Joint Work

Screening of alpha-helical peptide ligands controlling a calcineurin-phosphatase activity Reviewed

Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 17 ( 1 ) 167 - 171 2007.1

Publisher：PERGAMON-ELSEVIER SCIENCE LTD

In this paper, we describe an application of 202-membered fluorescently labeled peptide library designed to take an alpha-helix secondary structure. As a proof-of-concept experiment, a calmodulin (CaM)/calcineurin (Cn) pair was chosen to screen alpha-helical peptide ligands that tightly bind to CaM and also control enzymatic functions of Cn. Three peptides were successfully selected from the library by assaying Cn-phosphatase activities and peptide-CaM interactions (dual check process). The strategy using a designed peptide. library shows real promise as a peptide-based high-throughput screening system. (c) 2006 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.bmcl.2006.09.075

PubMed

Molecular Imprinting Under Molecular Crowding Conditions: an Aid to the synthesis of a High-capacity Polymeric Sorbent for Triazine Herbicides

J. Matsui, S. Goji, T. Murashima, D. Miyoshi, S. Komai, A. Shigeyasu, T. Kushida, T. Miyazawa, T. Yamada, K. Tamaki, N. Sugimoto

Anal. Chem. 2007

Joint Work

Authorship：Lead author

Synthesis, structure and thermal stability of fully hydrophobic porphyrin-DNA conjugates Reviewed

T. Murashima, K. Hayata, Y. Saiki, J. Matsui, D. Miyoshi, T., Yamada, T. Miyazawa, and N. Sugimoto

Tetrahedron Letters 2007

Joint Work

Neural-tube specific paralogous genes and their upstream regulatory sequences

西方敬人

Mem. Konan Univ., Sci. Eng. Ser ( 54 ) 75 - 88 2007

Single Work

Controlled magnetic properties of Ni nanoparticles embedded in polyimide films

S. Tomita, P. E. Jonsson, K. Akamatsu, H. Nawafune, H. Takayama

Phys. Rev. B ( 174432 ) 1 - 6 2007

Joint Work

ポリエチレングリコール誘導体の予備吸着を利用する銅電析プロセスによるULSI微細配線の形成

尾山祐斗、有村英俊、赤松謙祐、縄舟秀美

表面技術 58 425 - 429 2007

Joint Work

Copper/polyimide heterojunction: controlling interfacial structures through an additive-based, all-wet chemical process using ion-doped precursors

S. Ikeda, H. Yanagimoto, K. Akamatsu, H. Nawafune

Adv. Funct. Mater. 17 889 - 897 2007

Joint Work

One-step synthesis of polypyrrole-coated silver nanocomposite particles and their application as a coloured particulate emulsifier

S. Fujii, A. Aichi, K. Akamatsu, H. Nawafune and Y. Nakamura

J. Mater. Chem. 17 3777 - 3779 2007

Joint Work

Atomic Beam-Induced Fluorination of Polyimide and Its Application to Site-Selective Cu Metallization

M. Tagawa, K. Maeda, T. Kajita, K. Yokota, K. Akamatsu, H. Nawafune

Langmuir 23 11351 - 11354 2007

Joint Work

Photosensitization of oxygen molecules by surface-modified hydrophilic porus Si

M. Fujii, N. Nishimura, H. Fumon, S. Hayashi, K. Akamatsu, T. Tsuruoka, M. Shimada, H. Katayama, D. Kovalev, B. Goller

Euro. Phys. J. D 43 193 - 196 2007

Joint Work

Interactions between peptides containing nucleobase amino acids and T7 phages displaying S-cerevisiae proteins Reviewed

Sinya Watanabe, Kin-ya Tomizaki, Tsuyoshi Takahashi, Kenji Usui, Kotaro Kajikawa, Hisakazu Mihara

BIOPOLYMERS 88 ( 2 ) 131 - 140 2007

Joint Work

Publisher：JOHN WILEY & SONS INC

DOI： 10.1002/bip.20662

PubMed

Photosensitization of Oxygen Molecules by Surface-Modified Hydrophilic Porus Si Reviewed

M. Fujii, N. Nishimura, H. Fumon, S. Hayashi, K. Akamatsu, T. Tsuruoka, M. Shimada, H. Katayama, D. Kovalev, B. Goller

Euro. Phys. J. D 43 193 - 196 2007

Joint Work

Functional role of the cofactor on activation process of L-histidine dependent ribozyme（共著）

J. Kawakami, C. Maiya, and N. Sugimoto

Nucleic Acids Symp. Ser. 50 241 - 242 2006.11

Joint Work

Authorship：Lead author

Galactose-PEG Dual Conjugation of b-(1→3)-D-glucan schizophyllan for antisense oligonucleotide delivery to enhance cellular uptake

甲元一也

Biomaterials, ( 27 ) 1626 - 1635 2006.11

Single Work

CpG DNA/zymosan complex to enhance cytokine secretion owing to the cocktail effect

甲元一也

Bioorg. Med. Chem. Lett ( 16 ) 1301 - 1304 2006.11

Single Work

A DNA duplex with Extremely Enhanced Thermal Stability Based on Controlled Immobilization on Gold Nanoparticles.

三好大輔

Nano Lett., ( 6 ) 491 - 495 2006.11

Single Work

Development of molecular logic gates using the structural switch of telomere DNAs

三好大輔

Nucleic Acids Symp. Ser. ( 50 ) 315 - 316 2006.11

Single Work

Molecularly Imprinting within a Single Peptide Molecule for Synthesizing a Water-soluble ATP Receptor

三好大輔

Proceedings of the International Conference of 43rd Japanese Peptide Symposium and 4th Peptide Engineering Meeting 396 - 397 2006.11

Single Work

Properties of long telomeric DNAs under cell-mimicking conditions

三好大輔

Nucleic Acids Symp. Ser. ( 50 ) 207 - 208 2006.11

Single Work

Factors regulating thermodynamic stability of DNA structures under molecular crowding conditions

三好大輔

Nucleic Acids Symp. Ser ( 50 ) 203 - 204 2006.11

Single Work

Characterization of Structure and Stability of Long Telomeric DNA G-Quadruplexes

三好大輔

J. Am. Chem. Soc., ( 128 ) 15461 - 15468 2006.11

Single Work

DNA Logic Gates Based on Structural Polymorphism of Telomere DNA Molecules Responding to Chemical Input Signals

三好大輔

Angew. Chem. Int. Ed., ( 45 ) 7716 - 7719 2006.11

Single Work

Hydration regulates thermodynamics of G-quadruplex formation under molecular crowding conditions

三好大輔

J. Am. Chem. Soc ( 128 ) 7957 - 7963 2006.11

Single Work

生命を制御するのは遺伝子か環境か－細胞内環境に左右される遺伝子の構造と機能－

三好大輔

高分子 55 ( 5 ) 322 - 325 2006.11

Single Work

Effect of molecular crowding on DNA polymerase activity

三好大輔

Biotech. J., ( 1 ) 440 - 446 2006.11

Single Work

Properties of long telomeric DNAs under cell-mimicking conditions

三好大輔

Nucleic Acids Symp. Ser. ( 50 ) 207 - 208 2006.11

Single Work

The Suppression of Cell and Platelet Adhesion to Star-Shaped Poly(ethylene glycol)-Poly(L-lacitde) Block Copolymer Film, Reviewed

K. Nagahama, Y. Ohya, T. Ouchi,

Macromol. Biosci. 6 ( 6 ) 412 - 419 2006.11

Joint Work

Authorship：Lead author

Synthesis of Star-Shaped 8arms Poly(ethylene glycol)-Poly(L-lacitde) Block Copolymer and Physical Properties of Its Solution Cast Film as Soft Biomaterial Reviewed

K. Nagahama, Y. Ohya, T. Ouchi,

Polym. J., 38 ( 8 ) 852 - 860 2006.11

Joint Work

Authorship：Lead author

The roles of cosolutes on the hammerhead ribozyme activity

中野　修一

Nucleic Acids Symp. Ser. 50 81 - 82 2006.11

Joint Work

The effect of the structure of cosolutes on the DNA duplex formation

中野　修一

Nucleic Acids Symp. Ser. 50 205 - 206 2006.11

Joint Work

Recognition of the base pair-mimic nucleosides by DNA polymerases

中野　修一

Nucleic Acids Symp. Ser. 50 201 - 202 2006.11

Joint Work

Protein-fingerprint data mining of a designed alpha-helical peptide array Reviewed

Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara

MOLECULAR BIOSYSTEMS 2 ( 9 ) 417 - 420 2006.9

Publisher：ROYAL SOC CHEMISTRY

The data generated from protein fingerprints with an a-helical peptide array were analyzed using several statistical methods such as hierarchical clustering analysis and principal component analysis to discriminate target proteins. The data generated from protein fingerprints with an a-helical peptide array were analyzed using several statistical methods such as hierarchical clustering analysis and principal component analysis to discriminate target proteins.

DOI： 10.1039/b608875a

PubMed

A novel stable RNA pentaloop that interacts specifically with a motif peptide of lambda-N protein（共著） Reviewed

J. Kawakami, H. Tokitoh, Y. Tanabe, and N. Sugimoto

Nucleoside, Nucleotides and Nucleic Acids 25 397 - 416 2006.4

Joint Work

Authorship：Lead author

DOI： 10.1080/15257770600684027

The negative regulation of p53 by hedgehog signaling Reviewed

Abe Yoshinori, Oda-Sato Eri, Tobiume Kei, Kawauchi Keiko, Okamoto Koji, Taya Yoichi, Tanaka Nobuyuki

CANCER RESEARCH 66 ( 8 ) 2006.4

Joint Work

A novel peptide microarray for protein detection and analysis utilizing a dry peptide array system Reviewed

K Usui, K Tomizaki, T Ohyama, K Nokihara, H Mihara

MOLECULAR BIOSYSTEMS 2 ( 2 ) 113 - 121 2006.2

Publisher：ROYAL SOC CHEMISTRY

A novel dry peptide microarray system has been constructed that affords a practical solution for protein detection and analysis. This system is an array preparation and assay procedure tinder dry conditions that uses designed peptides as non-immobilized capture agents for the detection of proteins. The system has several advantages that include its portability and ease-Of-Use, as well as the fact that vaporization of sample solutions need not be considered. In this study, various proteins have been characterized with an a-helical peptide mini-library. When proteins were added to the peptide library array, the fluorescent peptides showed different fluorescent intensities depending on their sequences. The patterns of these responses could be regarded as protein fingerprints' (PFPs), which are sufficient to establish the identities of the target proteins. Furthermore, statistical analysis of the resulting PFPs was performed using cluster analysis. The PFPs of the proteins were clustered successfully depending on their families and binding properties. Additionally, the target protein was characterized using a nanolitre system and could be detected down to 1.2 fmol. These Studies imply that the dry peptide array system is a promising too] for detecting and analyzing target proteins. The dry peptide array will play a role in development of high-throughput protein-detecting nano/micro arrays for proteomics and ligand screening studies.

DOI： 10.1039/b514263r

PubMed

A PNA-DNA hybridization chip approach for the detection of beta-secretase activity Reviewed

S Sano, KY Tomizaki, K Usui, H Mihara

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 16 ( 3 ) 503 - 506 2006.2

Publisher：PERGAMON-ELSEVIER SCIENCE LTD

Developed was the addressable chip technology based on the PNA-DNA complementary hybridization equipped with short seven-mer PNA-encoded peptides that can be a versatile scaffold to monitor on-chip immunoassays. We also developed and validated a methodology to perform beta-secretase enzyme assay with a highly sensitive fashion, resulting that a peptide substrate tethering dual fluorescent probes allowed us to detect beta-secretase activity 10 times more sensitively than assays in solution. (c) 2005 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.bmcl.2005.10.064

PubMed

A DNA duplex with extremely enhanced thermal stability based on controlled immobilization on gold nanoparticles Reviewed

K. Akamatsu, M. Kimura, Y. Shibata, S. Nakano, D. Miyoshi, H. Nawafune, and N. Sugimoto

Nano Lett. 3 491 - 495 2006.1

Joint Work

DOI： 10.1021/nl0524748

Use of the polymorphic nature of high-order DNA structures for the design of dynamic and functional nanodevices Invited Reviewed

D. Miyoshi, N. Sugimoto

Recent Devel. Nucleic Acids Res. 2 49 - 70 2006.1

Joint Work

Use of the polymorphic nature of high-order DNA structures for the design of dynamic and functional nanodevices

D. Miyoshi, N. Sugimoto

Recent Devel. Nucleic Acids Res. 2 49 - 70 2006.1

Joint Work

Photodimerization of Anthryl Moieties in a Poly(methacrylic acid) Derivative as Reversible Cross-linking Step in Molecular Imprinting

J. Matsui, Y. Ochi, K. Tamaki

Chem. Lett. 80 - 81 2006

Joint Work

Authorship：Lead author

Molecularly-Imprinted Polymeric Logic Gates Selective for Predetermined Chemical Input Species

松井淳

Chem. Comm. 3217 - 3219 2006

Single Work

Dynamic redistribution of vasa homolog and exclusion of somatic cell determinants during germ cell specification in Ciona intestinalis.

西方敬人

Development ( 133 ) 2683 - 2693 2006

Single Work

UV-irradiated cells as a screening system for the antioxidants.

西方敬人

Proceedings of the 13th Biennial Congress of the International Society 　for Free Radical Research -SFRR 253 - 256 2006

Single Work

A DNA duplex with Extremely Enhanced Thermal Stability Based on Controlled Immobilization on Gold Nanoparticles

K. Akamatsu, M. Kimura, Y. Shibata, S. Nakano, D. Miyoshi, H. Nawafune, N. Sugimoto

Nano Lett. 6 491 - 495 2006

Joint Work

金属イオンレドックス系無電解めっき浴の安定性

赤松謙祐、増田昭仁、尾山祐斗、縄舟秀美

表面技術 57 799 - 801 2006

Joint Work

Fabrication of fluorescent multilayers consisting of CdTe nanocrystals

T. Tsuruoka, R. Takahashi, K. Akamatsu, H. Nawafune

Trans. Mater. Res. Soc. Jpn. 31 425 - 428 2006

Joint Work

Designed peptide microarrays for protein detection and characterization Reviewed

Kenji Usui, Kin-ya Tomizaki, Kiyoshi Nokihara, Hisakazu Mihara

UNDERSTANDING BIOLOGY USING PEPTIDES 731 - + 2006

Publisher：SPRINGER

DOI： 10.1007/978-0-387-26575-9_324

High throughput preparation of peptide arrays containing two fluorescent byes focusing on practical protein detection systems Reviewed

Kiyoshi Nokihara, Takafumi Ohyama, Koichi Yonemura, Yasuo Oka, Kenji Usui, Hisakazu Mihara

UNDERSTANDING BIOLOGY USING PEPTIDES 738 - + 2006

Joint Work

Publisher：SPRINGER

Site-Selective Integration of Monolayer-Protected Inorganic Nanoparticles onto Surface Monolayer Templates by a Solvent-Induced Lift-Off Process Reviewed

K. Akamatsu, S. Samitsu, T. Tsuruoka, J. Hasegawa, H. Nawafune

Small 2 1130 - 1133 2006

Joint Work

Fabrication of Fluorescent Multilayers Consisting of CdTe Nanocrystals Reviewed

T. Tsuruoka, R. Takahashi, K. Akamatsu, H. Nawafune

Trans. Mater. Res. Soc. Jpn. 31 425 - 428 2006

Joint Work

DNA base flipping by a base pair-mimic nucleoside Reviewed

S. Nakano, Y. Uotani, K. Uenishi, M. Fujii, and N. Sugimoto

Nucleic Acids Research, 33 7111 - 7119 2005.12

Single Work

DOI： 10.1093/nar/gki1018

Anomalous reflection of gold applicable for a practical protein-detecting chip platform Reviewed

S Watanabe, K Usui, K Tomizaki, K Kajikawa, H Mihara

MOLECULAR BIOSYSTEMS 1 ( 5-6 ) 363 - 365 2005.12

Publisher：ROYAL SOC CHEMISTRY

A simple, convenient and label-free fiber optic detection system based on the characteristic property, 'anomalous reflection (AR)' of gold was developed and preliminary experiments showed that the AR signals were sensitive enough to monitor protein-peptide interactions on solid surfaces.

DOI： 10.1039/b513075c

PubMed

Amino Acid Ester Salt Recognition by Ferrocene-based Ditopic Receptor Bearing Oligoethylene Glycol with Pendant Bipy Subunits: CV, UV-vis and ESR Studies. Reviewed

Murashima, T., Tsukiyama, S., Fujii, S., Hayata, K., Sakai, H., Miyazawa, T., Yamada, T.

Org. Biomol. Chem., 3 ( 22 ) 4060 - 4064 2005.11

Joint Work

Authorship：Lead author

Org. Biomol. Chem., （学術雑誌、2005）3, 4060-4064.

“Amino acid ester salt recognition by ferrocene-based ditopic receptor bearing oligoethylene glycol with pendant bipy subunits: CV, UV-vis and ESR Studies” Reviewed

Takashi Murashima, Sayo Tsukiyama, Satoshi Fujii, Kazuyuki Hayata, Hiroshi Sakai, Toshifumi Miyazawa, and Takashi Yamada

Org. Biomol. Chem 3 4060 - 4064 2005.11

Joint Work

In situ monitoring of polysaccharide-polynucleotide interaction using a schizophyllan-immobilized QCM device

甲元一也

Sensors & Actuators B: Chemicals ( 105 ) 490 - 494 2005.11

Single Work

Transferrin-appended b-(1→3)-D-glucan schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake

甲元一也

J. Contr. Rel. ( 108 ) 529 - 539 2005.11

Single Work

Transferrin-appended b-(1→3)-D-glucan schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake

甲元一也

e-J. Surface Sci. Nanotech ( 3 ) 195 - 202 2005.11

Single Work

Cholesterol-appended b-(1,3)-D-glucan schizophyllan for antisense oligonucleotide delivery to enhance cellular uptake

甲元一也

Bull. Chem. Soc. Jpn. ( 78 ) 1821 - 1830 2005.11

Single Work

Parallel vs. anti-parallel orientation in a curdlan/oligo(dA) complex as estimated by a FRET technique

甲元一也

Org. Biomol. Chem., ( 3 ) 2255 - 2261 2005.11

Single Work

Competition between Polysaccharide/Polynucleotide Complexation vs. Polynucleotide Hybridization; Salt Concentration Dependence of the Reaction Direction

甲元一也

e-J. Surface Sci. Nanotech. ( 3 ) 30 - 37 2005.11

Single Work

PEG-appended b-(1→3)-D-glucan schizophyllan to deliver antisense-oligonucleotides with avoiding lysosomal degradational of New Modification Technique for Linear Double-Stranded DNAs Using the Polysaccharide Schizophyllan

甲元一也

Biomaterials ( 26 ) 4866 - 4873 2005.11

Single Work

Schizophyllan-Poly(N-isopropylacrylamide) Conjugate for Selective Recovery of Polynucleotides by Precipitation

甲元一也

Polym. J., ( 37 ) 177 - 185 2005.11

Single Work

Thermodynamic and Kinetic Analysis of Nucleic Acid Structures toward Pharmacogenomics.

三好大輔

Current Pharmacogenomics, ( 3 ) 217 - 236 2005.11

Single Work

Structural switch of telomere DNA by pH and monovalent cation.

三好大輔

Nucleic Acids Symp. Ser ( 49 ) 243 - 244 2005.11

Single Work

Structure and stability of DNA quadruplexes under molecular crowding conditions.

三好大輔

Nucleic Acids Symp. Ser ( 49 ) 239 - 240 2005.11

Single Work

DNA nanowire sensitive to the surrounding condition.

三好大輔

Nucleic Acids Symp. Ser. ( 49 ) 43 - 44 2005.11

Single Work

DNA-Based Biosensor for Monitoring pH in Vitro and in Living Cells.

三好大輔

Biochemistry ( 44 ) 7125 - 7130 2005.11

Single Work

Roles of Mg2+ in TPP-dependent riboswitch.

三好大輔

FEBS Lett. ( 579 ) 2583 - 2588 2005.11

Single Work

Drastic Effect of a Single Base Difference between Human and Tetrahymena Telomere Sequences on Their Structures under Molecular Crowding Conditions.

三好大輔

Angew. Chem. Int. Ed. Engl ( 44 ) 3740 - 3744 2005.11

Single Work

SPR sensor chip for detection of small molecules using molecularly imprinted polymer with embedded gold nanoparticles.

三好大輔

Anal Chem ( 77 ) 4282 - 4285 2005.11

Single Work

Roles of Mg2+ in TPP-dependent riboswitch.

三好大輔

FEBS Lett ( 579 ) 2583 - 2588 2005.11

Single Work

Thermodynamic and Kinetic Analysis of Nucleic Acid Structures toward Pharmacogenomics

三好大輔

Current Pharmacogenomics ( 3 ) 217 - 236 2005.11

Single Work

Drastic effect of a single base difference between human and tetrahymena telomere sequences on their structures under molecular crowding conditions.

三好大輔

Angew Chem Int Ed Engl. ( 44 ) 3740 - 3744 2005.11

Single Work

SPR Sensor Chip for Detection of Small Molecules Using Molecularly Imprinted Polymer with Embedded Gold Nanoparticles　Anal. Chem.

松井淳

（学術雑誌、 2005 ）77, 4282-4285 4282 - 4285 2005.11

Single Work

Synthesis of Water-soluble Porphyrin and the Corresponding Highly Planar Benzoporphyrin without meso-Substituents.

村嶋　貴之

Tetrahedron Lett., 2005.11

Single Work

Tetrahedron Lett., （学術雑誌、2005）46, 113-116.

Resolution of Non-protein Amino Acids via Carica papaya Lipase-catalyzed Enantioselective Transesterification.

村嶋　貴之

Tetrahedron: Asymmetry, 2005.11

Single Work

Tetrahedron: Asymmetry, （学術雑誌、2005）16, 2569-2573.

Enzymatic Resolution of 2-Aryloxy-1-propanols via Lipase-catalyzed Enantioselective Acylation Using Acid Anhydrides as Acyl Donors.

村嶋　貴之

J. Mol. Cat., B, 2005.11

Single Work

J. Mol. Cat., B, （学術雑誌、2005）37, 63-67.

Studies on intramolecular hydrogen bonding between the pyridine nitrogen and the amide hydrogen of the peptide: synthesis and conformational analysis of tripeptides containing novel amino acids with a pyridine ring.

村嶋　貴之

J. Peptide Sci., 2005.11

Single Work

J. Peptide Sci., （学術雑誌、2005）11, 491-498.

Conformational switch of oligonucleotides induced by spermine

中野　修一

Nucleic Acids Symp. Ser. 49 241 - 242 2005.11

Single Work

Linkage between proton binding and folding in RNA: implications for RNA catalysis

中野　修一

Biochem. Soc. Trans. 33 466 - 470 2005.11

Single Work

A designed glycopeptide array for characterization of sugar-binding proteins toward a glycopeptide chip technology Reviewed

Kenji Usui, Tetsunori Ojima, Kin-Ya Tomizaki, Hisakazu Mihara

Nanobiotechnology 1 ( 2 ) 191 - 199 2005.11

For the realization of a practical high-throughput protein detection and analysis system, a novel peptide array has been constructed using a designed glycopeptide model library with an α-helical secondary structure. This study will contribute the increment of the diversity of such an array system and the application to focused proteomics and ligand screening by effective detection of sugar-binding proteins. Fluorescent glycopeptides with an α-helix, a β-strand, or a loop structure were designed initially to select a suitable scaffold for the detection of a model protein. After selection of the α-helical structure as the best scaffold, a small model library with various saccharides was constructed to have charge and hydrophobicity variations in the peptide sequences. When various sugar-binding proteins were added to the peptide library array, the fluorescent peptides showed different responses in fluorescence intensities depending on their sequences as well as saccharides. The patterns of these responses could be regarded as "protein fingerprints" (PFPs), which are able to establish the identities of the target proteins. The resulting PFPs reflected the recognition properties of the proteins. Furthermore, statistical data analysis from obtained PFPs was performed using a cluster analysis. The PFPs of sugar-binding proteins were clustered successfully depending on their families and binding properties. These studies demonstrate that arrays with glycopeptide libraries based on designed structures can be promising tools to detect and analyze the target proteins. Designed peptides with functional groups such as sugars will play roles as the capturing agents of high-throughput protein nano/micro arrays for focused proteomics and ligand screening studies. Copyright © 2005 Humana Press Inc. All rights of any nature whatsoever are reserved.

DOI： 10.1385/NBT:1:2:191

Difference in self-assembling morphology of peptide nanorings Reviewed

H Okamoto, T Yamada, H Miyazaki, T Nakanishi, K Takeda, K Usui, Obataya, I, H Mihara, H Azehara, W Mizutani, K Hashimoto, H Yamaguchi, Y Hirayama

JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS 44 ( 11 ) 8240 - 8248 2005.11

Publisher：JAPAN SOC APPLIED PHYSICS

We synthesized the peptide nanorings of cyclo[-(D-Ala-L-Gln)(3)], cyclo[-(D-CyS-L-Gln)(3)], CyC10[-D-Cys-L-HiS-D-Ala-L-Asn-Gly-L-Gln-1 and Cyc1o[-(L-Gln)(5)], and studied the way in which the difference in the type and/or number of component amino acid residues changes the self-assembling morphology of the nanorings on gold substrates by atomic force microscopy. The study revealed that CyClo[-(D-Ala-L-Gln)(3)] formed nanotube bundles through inter-ring hydrogen bonds, while the nanorings of CyC10[-(D-CyS-L-Gln)3] adhered to the gold surface directly due to the high affinity of thiol to gold. In contrast, a random amino acid sequence of cyclo[-D-CyS-L-HiS-D-Ala-L-Asn-GlY-L-Gln-] resulted in many isolated nanotubes, which were first observed in the present study. While the D,L-peptide nanotubes have very straight forms, the homo-L-peptide of cyclo[-(L-Gln)(5)] formed interesting randomly branching nanotubes that were entwined and grew on the substrate. Scanning tunneling microscopy was also performed and high-resolution images of both the peptide nanotubes and the nanotube bundles were obtained.

DOI： 10.1143/JJAP.44.8240

IR study on stacking manner of peptide nanorings in peptide nanotubes Reviewed

Y Nagai, T Nakanishi, H Okamoto, K Takeda, Y Furukawa, K Usui, H Mihara

JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS 44 ( 10 ) 7654 - 7661 2005.10

Publisher：JAPAN SOC APPLIED PHYSICS

We here report our theoretical as well as experimental studies on the stacking manner of peptide nanorings (PNRs) in peptide nanotubes (PNTs). We focus on the molecular vibrations of N-H and C=O stretching modes and discuss this subject via their infrared (IR) spectroscopy, because PNTs are formed by the inter-ring H bonds between the adjacent PNRs via -N-H(...)O=C-. Symmetry analysis based on group theory reveals that parallel stacking causes two IR active modes in these molecular vibrations while three modes are active in the antiparallel stacking. This difference in the number of IR active modes is determined only by the stacking manner and not by the number of amino acid residues composing the PNRs. By using two typical PNRs Of cyclo[-((L)-Gln-D-Ala)(3)] and cyclo[-((L)-Gln-(D)-Ala)(4)], we further studied the favorable stacking manners of PNRs via IR observation. Our IR experiments as well as the ab initio energetics show that the former PNRs create a PNT by stacking themselves in parallel while the latter PNRs do so by stacking themselves in an antiparallel manner.

DOI： 10.1143/JJAP.44.7654

Specific recognition of the basic N peptide by an RNA（共著）

J. Kawakami, H. Tokitoh, Y. Tanabe, and N. Sugimoto

Nucleic Acids Symposium Series 49 353 - 354 2005.9

Joint Work

Authorship：Lead author

Central dogma for a molecular design based on DNA: DNB (databasing/designable nanobio) -> ENB (engineering nanobio) -> FNB (functional nanobio) Reviewed

S. Nakano and N. Sugimoto

Chemistry Letters 34 1206 - 1211 2005.7

Single Work

DOI： 10.1246/cl.2005.1206

Protein-detecting microarrays: Current accomplishments and requirements Reviewed

Kin-Ya Tomizaki, Kenji Usui, Hisakazu Mihara

ChemBioChem 6 ( 6 ) 782 - 799 2005.5

Joint Work

The sequencing of the human genome has been successfully completed and offers the chance of obtaining a large amount of valuable information for understanding complex cellular events simply and rapidly in a single experiment. Interestingly, in addressing these proteomic studies, the importance of protein-detecting microarray technology is increasing. In the coming few years, microarray technology will become a significantly promising and indispensable research/diagnostic tool from just a speculative technology. It is clear that the protein-detecting microarray is supported by three independent but strongly related technologies (surface chemistry, detection methods, and capture agents). Firstly, a variety of surface-modification methodologies are now widely available and offer site-specific immobilization of capture agents onto surfaces in such a way as to keep the native conformation and activity. Secondly, sensitive and parallel detection apparatuses are being developed to provide highly engineered microarray platforms for simultaneous data acquisition. Lastly, in the development of capture agents, antibodies are now probably the most prominent capture agents for analyzing protein abundances. Alternative scaffolds, such as phage-displayed antibody and protein fragments, which provide the advantage of increasing diversity of proteinic capture agents, however, are under development. An approach involving recombinant proteins fused with affinity tag(s) and coupled with a highly engineered surface chemistry wilt provide simple production protocols and specific orientations of capture agents on the -microarray formats. Peptides and other small molecules can be employed in screening highly potent ligands as well as in measuring enzymatic activities. Protein-detecting microarrays supported by the three key technologies should contribute in accelerating diagnostic/biological research and drug discovery. © 2005 Wiley-VCH Verlag GmbH &amp
Co. KGaA.

DOI： 10.1002/cbic.200400232

PubMed

Studies on Fluorescent Dyes and Surface Chemistry focusing on Practical Peptide Array Preparation Reviewed

NOKIHARA Kiyoshi, USUI Kenji, YONEMURA Koichi, OHYAMA Takafumi, OKA Yasuo, TOMIZAKI Kin‐ya, MIHARA Hisakazu

Pept Sci 2004 145 - 148 2005.3

Joint Work

J-GLOBAL

Tyrosine phosphorylation of adaptor protein 3BP2 induces T cell receptor-mediated activation of transcription factor.

Qu X, Kawauchi-Kamata K, Miah SM, Hatani T, Yamamura H, Sada K

Biochemistry 44 ( 10 ) 3891 - 3898 2005.3

Joint Work

DOI： 10.1021/bi048353o

PubMed

Thermodynamic analysis of duplex formation of the heterochiral DNA with L-deoxyadenosine（共著） Reviewed

J. Kawakami, K. Tsujita, and N. Sugimoto

Analytical Sciences 21 ( 2 ) 77 - 82 2005.2

Joint Work

Authorship：Lead author

Site-selective RNA cleavage by DNA bearing a base pair-mimic nucleoside Reviewed

S. Nakano, Y. Uotani, K. Uenishi, M. Fujii, and N. Sugimoto

J. Am. Chem. Soc. 127 518 - 519 2005.1

Single Work

DOI： 10.1021/ja045445s

Nucleic acid with guanidinium modification exhibits efficient cellular uptake.

西方敬人

Angew. Chem. Int. Ed. Engl ( 44 ) 6682 - 6685 2005

Single Work

Site-Selective Deposition of Gold on Glass Substrates Using Monolayer-Protected Gold Nanoparticles

K. Akamatsu, H. Terahata, J. Hasegawa, H. Nawafune

J. Surf, Finish, Soc. Jpn 56 427 - 428 2005

Joint Work

（学術雑誌、2005）56, 427-428

Fabrication and Structural Control of Fe/Ti Oxide Thin Films with Graded Compositional Profiles by Liquid Phase Deposition

S. Deki, S. Iizuka, K. Akamatsu, M. Mizuhata and A. Kajinami

J. Ame. Ceram. Soc. 88 731 - 736 2005

Joint Work

（学術雑誌、2005) 88, 731-736

Selective Electroless Copper Deposition on Aluminum Nitride Substrate with Patterned Copper Seed Layer

H. Yanagimoto, S. Deki, K. Akamatsu, and K. Gotoh

Thin Solid Films 491 18 - 22 2005

Joint Work

（学術雑誌、2005) 491, 18-22

A novel fabrication technique for interacting ferromagnetic-metal nanoparticle systems:fine-tuning of particle dimeter and interparticle spacing

S. Tomita, K. Akamatsu, H. Shinkai, S. Ikeda, H. Nawafune, C. Mitsumata, T. Kashiwagi, M. Hagiwara

Mater. Res. Soc. Proceedings 853E I5.10.1 - I5.10.6 2005

Joint Work

（学術雑誌、2005） 853E. I5.10.1-I5.10.6

Site-Selective Deposition of Gold on Glass Substrates Using Monolayer-Protected Gold Nanoparticles

K. Akamatsu, H. Terahata, J. Hasegawa, H. Nawafune

J. Surf. Finish. Soc. Jpn 56 427 - 428 2005

Joint Work

（学術雑誌、2005）56. 427-428

Band Gap Engineering of CdTe nanocrystals Through Chemical Surface Modification

K. Akamatsu, T. Tsuruoka, H. Nawafune

J. Am. Chem. Soc. 127 1634 - 1635 2005

Joint Work

（学術雑誌、2005） 127. 1634-1635

Stereocontrolled Synthesis and Optical Properties of All-cis Poly(phenylene vinylene)s (PPVs): A Method for Direct Patterning of PPVs

H. Katayama, M. Nagao, T. Nishimura, Y. Matsui, K. Umeda, K. Akamatsu, T. Tsuruoka, H. Nawafune, F. Ozawa

J. Am. Chem. Soc. 127 4350 - 4353 2005

Joint Work

（学術雑誌、2005） 127. 4350-4353

An SPR Sensor Chip for Sensitive Detection of Small Molecules Using Molecularly Imprinted Polymer with Embedded Gold Nanoparticle

J. Matsui, K. Akamatsu, N. Hara, D. Miyoshi, H. Nawafune, K. Tamaki, N. Sugimoto

Anal. Chem. 77 4282 - 4285 2005

Joint Work

（学術雑誌、2005） 77. 4282-4285

Controlling Interparticle Spacing Among Metal Nanoparticles Through Metal-Catalyzed Decomposition of Surrounding Polymer Matrix

K. Akamatsu, H. Shinkai, S. Ikeda, S. Adachi, H. Nawafune, S. Tomita

J. Am. Chem. Soc. 127 7980 - 7981 2005

Joint Work

（学術雑誌、2005） 127. 7980-7981

Tuning magnetic interactions in ferromagnetic-metal nanoparticle systems

S. Tomita, K. Akamatsu, H. Shinkai, S. Ikeda, H. Nawafune, C. Mitsumata, T. Kashiwagi, and M, Hagiwara

Phys. Rev. B 71 180414(R)1 - 4 2005

Joint Work

（学術雑誌、2005） 71. 180414(R)1-4

Surface Functionalization of Mixed Monolayer-Protected Semiconductor Nanocrystals

T. Tsuruoka, K. Akamatsu, H. Nawafune

Trans. Mater. Res. Soc 30 1227 - 1230 2005

Joint Work

（学術雑誌、2005） 30, 1227-1230

Synthesis and Structural Control of Metal-Containing Polyimide Nanocomposite

H. Shinkai, S. Ikeda, K. Akamatsu, H. Nawafune, S. Tomita

Trans. Mater. Res. Soc. Jpn. 30 1223 - 1226 2005

Joint Work

（学術雑誌、2005）30, pp. 1223-1226

Site-Selective Direct Photochemical Deposition of Copper on Glass Substrates Using TiO₂ Nanocrystals

K. Akamatsu, A. Kimura, H. Matsubara, S. Ikeda, H. Nawafune

Langmuir 21 8099 - 8102 2005

Joint Work

（学術雑誌、2005）21, 8099-8102

Protein-detection microarrays using structure-based designed peptide libraries Reviewed

Kenji Usui, Tetsunori Ojima, Masato Suzuki, Sin-ya Watanabe, Kin-ya Tomizaki, Kiyoshi Nokihara, Hisakazu Mihara

Peptides 2004, Proceedings 401 - 402 2005

Joint Work

Publisher：KENES INT

Optimization of peptide arrays on chip as novel practical protein characterization system Reviewed

Kiyoshi Nokihara, Takafumi Ohyama, Koichi Yonemura, Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara

Peptides 2004, Proceedings 176 - 177 2005

Joint Work

Publisher：KENES INT

Band Gap Engineering of CdTe Nanocrystals Through Chemical Surface Modification Reviewed

K. Akamatsu, T. Tsuruoka, H. Nawafune

J. Am. Chem. Soc. 127 1634 - 1635 2005

Joint Work

Stereocontrolled Synthesis and Optical Properties of All-cis Poly(phenylene vinylene)s (PPVs): A Method for Direct Patterning of PPVs Reviewed

H. Katayama, M. Nagao, T. Nishimura, Y. Matsui, K. Umeda, K. Akamatsu, T. Tsuruoka, H. Nawafune, F. Ozawa

J. Am. Chem. Soc. 127 4350 - 4353 2005

Joint Work

Surface Functionalization of Mixed Monolayer-Protected Semiconductor Nanocrystals Reviewed

T. Tsuruoka, K. Akamatsu, H. Nawafune

Trans. Mater. Res. Soc. Jpn. 30 1227 - 1230 2005

Joint Work

Nickel- and Palladium-Catalyzed Cross-Coupling Reaction of Polyfluorinated Arenes and Alkenes with Grignard Reagents Reviewed

Tomoyuki Saeki, Yohei Takashima, Kohei Tamao

Synlett 11 1771 - 1774 2005

Joint Work

Duplex dissociation of telomere DNAs induced by molecular crowding. Reviewed

D. Miyoshi, S. Matsumura, S. Nakano, and N. Sugimoto

J. Am. Chem. Soc. 128 165 - 169 2004.12

Single Work

DOI： 10.1021/ja036721q

Introduction of peptide function into DNA by Nucleic Acid Peptides, NAPs（共著）

J. Kawakami, Z.-M. Wang, H. Fujiki, S. Izumi, and N. Sugimoto

Chemistry Letters 33 ( 12 ) 1554 - 1555 2004.12

Joint Work

Authorship：Lead author

DOI： 10.1246/cl.2004.1554

多糖・核酸複合体を用いた機能性高分子コンジュゲート　−核酸医薬デリバリーへの挑戦−

甲元一也

高分子加工 ( 53 ) 561 - 568 2004.11

Single Work

A Polysaccharide Carrier for Immunostimulatory CpG DNAs to Enhance Cytokine Secretion

甲元一也

J. Am. Chem. Soc. ( 126 ) 8372 - 8373 2004.11

Single Work

Removal of the side-chain glucose groups from schizophyllan improves the thermal stability of the polycytidylic acid complex under the physiological conditions

甲元一也

Biopolymers ( 75 ) 403 - 411 2004.11

Single Work

Schizophyllans carrying oligosaccharide appendages as potential candidates for cell-targeted antisense carrier

甲元一也

Org. Biomol. Chem ( 2 ) 3091 - 3098 2004.11

Single Work

Proposal of New Modification Technique for Linear Double-Stranded DNAs Using the Polysaccharide Schizophyllan

甲元一也

Bioorg. Med. Chem. Lett ( 14 ) 5655 - 5659 2004.11

Single Work

Molecular weight control of curdlan (b-1,3-glucan polysaccharide) provides unique polynucleotide binding properties

甲元一也

Polym. J. ( 36 ) 380 - 385 2004.11

Single Work

Low Mw sulfated curdlan with improved water-solubility forms macromolecular complexes with polycytidylic acid

甲元一也

Carbohydr. Res ( 339 ) 161 - 167 2004.11

Single Work

Chemically modified polysaccharide schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake efficiency

甲元一也

Biochim. Biophys. Acta ( 1670 ) 91 - 104 2004.11

Single Work

Lactose-appended schizophyllan as a potential candidate for hepatocyte-targetted antisense carrier

甲元一也

Chem. Commun 382 - 383 2004.11

Single Work

Protection of polynucleotides against nuclease-mediated hydrolysis by complexation with schizophyllan

甲元一也

Biomaterials ( 25 ) 3109 - 3116 2004.11

Single Work

Antisense oligonucleotides bound in the polysaccharide complex and the enhanced antisense effect due to the low hydrolysis

甲元一也

Biomaterials ( 25 ) 3117 - 3123 2004.11

Single Work

Long-Term Expression with Cationic Polymer Derived from a Natural Polysaccharide: Schizophyllan

甲元一也

Bioconjugate Chem ( 15 ) 249 - 259 2004.11

Single Work

Polysaccharide/Polynucleotide Complexes Part 6: Complementary-Strand-Induced Release of Single-stranded DNA Bound in the Schizophyllan Complex

甲元一也

Chemistry & Biodiversity ( 1 ) 520 - 529 2004.11

Single Work

Enhancement of antisense effect by reducing interactions between proteins and ODNs

甲元一也

Bull. Chem. Soc. Jpn. ( 77 ) 1101 - 1110 2004.11

Single Work

First observation by Fluorescence Polarization of Complexation between mRNA and the Natural Polysaccharide Schizophyllan

甲元一也

Chemistry & Biodiversity ( 1 ) 634 - 639 2004.11

Single Work

Duplex Dissociation of Telomere DNA Induced by Molecular Crowding.

三好大輔

J. Am. Chem. Soc. ( 126 ) 165 - 169 2004.11

Single Work

生命分子を用いたサブナノデバイスの創製

三好大輔

未来材料(NTS) ( 4 ) 26 - 33 2004.11

Single Work

Composite of Au Nanoparticles and molecularly imprinted polymer as a sensing material

三好大輔

Anal. Chem ( 76 ) 1310 - 1315 2004.11

Single Work

Synthetic Cinchonidine Receptors Obtained by Cross-linking Linear Poly(Methacrylic Acid) Derivatives as an Alternative Molecular Imprinting Technique　J. Chromatogr., B

松井淳

（学術雑誌、 2004 ）804, 223-229 223 - 229 2004.11

Single Work

Atrazine Tranformation Using Synthetic Enzymes Prepared by Molecular Imprinting　Org. Biomol. Chem.

松井淳

（学術雑誌、 2004 ）2, 2563-2566 2563 - 2566 2004.11

Single Work

Composite of Au Nanoparticles and molecularly imprinted polymer as a sensing material　　Anal. Chem.

松井淳

（学術雑誌、 2004 ）76, 1310-1315 1310 - 1315 2004.11

Single Work

Synthesis of Tripeptides Containing a Very Crowded a,a-Disubstituted Glycine with Pyridine Rings by Solid-Phase Ugi Reaction.

村嶋　貴之

Tetrahedron Lett, 2004.11

Single Work

Tetrahedron Lett, （学術雑誌、 2004 ）45, 8871-8874.

A novel intramolecular hydrogen bonding between a side-chain pyridine ring and an amide hydrogen of the peptide backbone in tripeptides containing the new amino acid, a,a-di(2-pyridyl)glycine.

村嶋　貴之

Org. Biomol. Chem., 2004.11

Single Work

Org. Biomol. Chem., （学術雑誌、 2004 ） 2, 2335-2339 .

Conformational change of a nucleotide by a base-pair mimic nucleoside in the complementary DNA strand

中野　修一

Nucleic Acids Symp. Ser. 48 259 - 260 2004.11

Single Work

Secondary structure change of oligonucleotides induced by salts

中野　修一

Nucleic Acids Symp. Ser. 48 109 - 110 2004.11

Single Work

Stabilization of a DNA duplex under molecular crowding conditions of PEG

中野　修一

Nucleic Acids Symp. Ser. 48 107 - 108 2004.11

Single Work

Peptide Arrays with Designed Secondary Structures for Protein Characterization Using Fluorescent Fingerprint Patterns. Reviewed

臼井健二

Biopolymers ( 76 ) 129 - 139 2004.11

Single Work

Stabilization of a DNA duplex under molecular crowding conditions of PEG （共著）

H. Karimata, S. Nakano, T. Ohmichi, J. Kawakami, and N. Sugimoto

Nucleic Acids Symp. Ser. 48 107 - 108 2004.11

Joint Work

Analysis of the secondary structure of a ribozyme that requires His as a cofactor（共著）

J. Kawakami, C. Maiya, and N. Sugimoto

Nucleic Acids Symp. Ser. 48 203 - 204 2004.11

Joint Work

Authorship：Lead author

The effect of molecular crowding with nucleotide length and cosolute structure on DNA duplex stability（共著） Reviewed

S. Nakano, H. Karimata, T. Ohmichi, J. Kawakami, and N. Sugimoto

Journal of American Chemical Society 126 14330 - 14331 2004.10

Joint Work

DOI： 10.1021/ja0463029

"Spinnokinetic Analyses of Blood Disposition and Biliary Excretion of Nitric Oxide (NO)-Fe(II)-N-(Dithiocarboxy)sarcosine Complex in Rats: BCM-ESR and BEM-ESR Studies" Reviewed

Hiroyuki Yasui, Satoshi Fujii, Tetsuhiko Yoshimura, and Hiromu Sakurai

Free Radic. Res 38 ( 10 ) 1061 - 1072 2004.10

Joint Work

Peptide arrays with designed alpha-helical structures for characterization of proteins from FRET fingerprint patterns Reviewed

Kenji Usui, Mizuki Takahashi, Kiyoshi Nokihara, Hisakazu Mihara

MOLECULAR DIVERSITY 8 ( 3 ) 209 - 218 2004.9

Publisher：SPRINGER

A practical high-throughput protein detection system is described, based on synthetic peptide arrays consisting of designed alpha-helical peptides, detected by fluorescence resonance energy transfer (FRET). Initially a model alpha-helical peptide known to interact with a structured protein, calmodulin, was selected to establish the strategy for high-throughput detection. In comparison to peptides with a single probe, a much higher FRET response has been observed with two fluorescent probes (7-diethylaminocoumarin-3-carboxylic acid and 5(6)-carboxy-fluorescein) at both termini of the synthetic peptides. To establish a reproducible high-throughput detection system, peptides were also immobilized onto a solid surface for detection of the target proteins. A small library of 112 different peptides was constructed, based on a model of the alpha-helical peptide with systematic replacement of residues carrying specific charges and/or hydrophobicities. The library was used to effectively characterize various proteins, giving their own 'protein fingerprint' patterns. The resulting 'protein fingerprints' correlate with the recognition properties of the proteins. The present microarray with designed synthetic peptides as the capturing agents is promising for the development of protein detection chips.

DOI： 10.1023/B:MODI.0000036237.82584.2d

PubMed

Cycloprodigiosin up-regulates inducible nitric oxide synthase gene expression in hepatocytes stimulated by interleukin-1beta.

Teshima S, Nakanishi H, Kamata K, Kaibori M, Kwon AH, Kamiyama Y, Nishizawa M, Ito S, Okumura T

Nitric oxide : biology and chemistry 11 ( 1 ) 9 - 16 2004.8

Joint Work

DOI： 10.1016/j.niox.2004.07.009

PubMed

Influences of ribonucleotide on a duplex conformation and its thermal stability: Study with the chimeric RNA-DNA strands. Reviewed

S. Nakano, T. Kanzaki, and N. Sugimoto

J. Am. Chem. Soc. 126 1088 - 1095 2004.1

Single Work

DOI： 10.1021/ja037314h

Catalytic roles for proton transfer and protonation in ribozymes: Current understanding and future possibilities. Reviewed

P. C. Bevilacqua, T. S. Brown, S. Nakano, and R. Yajima

Biopolymers 73 90 - 109 2004.1

Single Work

DOI： 10.1002/bip.10519

Fluorescent in situ hybridization to ascidian chromosomes.

西方敬人

Zool. Sci. ( 21 ) 153 - 157 2004

Single Work

A role of Ecdysteroid-phosphates during early embryonic development in silkworm Bombyx mori.

西方敬人

Mem. Konan Univ., Sci. Ser ( 51 ) 1 - 16 2004

Single Work

CiYB1 is a major component of storage mRNPs in ascidian oocytes: implications in translational regulation of localized mRNAs

西方敬人

Developmental Biology ( 272 ) 217 - 230 2004

Single Work

Synthesis, Surface Modification and Multilayer Construction of Mixed Monolayer-Protected CdS Nanoparticles

T. Tsuruoka, K. Akamatsu, H. Nawafune

Langmuir 20 11169 - 11174 2004

Joint Work

（学術雑誌、2004）3. 11169-11174

Electroless Copper Plating Using Sn(II) Complex as a Reducing Agent for ULSI Metallization

H. Nawafune, Y. Kobata, K. Akamatsu, S. Mizumoto, Y. Murakami

Mem. Konan Univ., Sci. Eng.Ser. 51 29 - 38 2004

Joint Work

（大学・研究所紀要、2004）51, 29-38

Preparation of transparent alumina film and fiber from a composite of aluminum polynuclear complex/methyl cellulose

Y. Gotoh, K. Fujimura, Y. Ohkoshi, M. Nagura, K. Akamatsu, and S. Deki

Mater. Chem. Phys. 83 54 - 59 2004

Joint Work

（学術雑誌、2004）83, 54-59

Aqueous solution-based synthesis of rare earth-doped metal oxide thin films

S. Deki, K. Kuratani, M. Uemura, K. Akamatsu, M. Mizuhata, and A. Kajinami

Thin Solid Films 460 83 - 86 2004

Joint Work

（学術雑誌、2004）460, 83-86

Polyaddition of 2,7-diethyl-9,9-dioctylfluorene using regio- and stereoselective alkyne dimerization catalysts

H. Katayama, M. Nakayama, T. Nakano, C. Wada, K. Akamatsu and F. Ozawa

Macromolecules 37 13 - 17 2004

Joint Work

（学術雑誌、2004）37, 13-17

Composite of Au nanoparticles and molecularly imprinted polymer as a sensing material

J. Matsui, K. Akamatsu, S. Nishiguchi, D. Miyoshi, H. Nawafune, K. Tamaki and N. Sugimoto

Anal. Chem. 76 1310 - 1315 2004

Joint Work

（学術雑誌、2004）76, 1310-1315

Direct Patterning of Copper on Polyimide Using Ion Exchangeable Surface Templates Generated by Site-Selective Surface Modification

K. Akamatsu, S. Ikeda, H. Nawafune, H. Yanagimoto

J. Am. Chem. Soc 126 10822 - 10823 2004

Joint Work

.（学術雑誌、2004）126, 10822-10823

Formation and Growth of Copper Nanoparticles from Ion-Doped Precursor Polyimide Layers

S. Ikeda, K. Akamatsu, H. Nawafune, T. Nishino, S. Deki

J. Phys. Chem. B. 108 15599 - 15607 2004

Joint Work

（学術雑誌、2004）108, 15599-15607

Development of a practical protein-chip using designed synthetic peptide-arrays Reviewed

K Nokihara, T Ohyama, K Usui, K Yonemura, K Tomizaki, H Mihara

KOBUNSHI RONBUNSHU 61 ( 10 ) 523 - 532 2004

Publisher：SOC POLYMER SCIENCE JAPAN

Novel high-throughput technologies, which can replace conventional electrophoresis and mass spectroscopic analyses, are in great demand for understanding the structure-function relationships of proteins. For a practical protein-detection system, arrays with immobilized designed synthetic peptides have been constructed. Peptides were labeled with fluorescent dyes in order to achieve high sensitivity. Two different types of prototype-arrayer have been constructed: one has a micro-dispensing system and the other a spot-printing system. Combinatorial peptide libraries, which consisted of beta-loop, beta-strand and alpha-helical peptides, were constructed by improved highly efficient solid-phase synthesis. Labeled peptides were covalently immobilized on solid supports such as precision glass plates. Various proteins were characterized with these peptide-arrays using a fluorescent scanner to give the "protein fingerprint" which is characteristic of the individual proteins. The results of the present prototype system demonstrate the practicality of protein-chips as a new generation of biochips.

Peptide arrays with designed secondary structures for protein characterization using fluorescent fingerprint patterns Reviewed

K Usui, T Ojima, M Takahashi, K Nokihara, H Mihara

BIOPOLYMERS 76 ( 2 ) 129 - 139 2004

Publisher：JOHN WILEY & SONS INC

To realize a practical high-throughput protein-detection system, novel peptide arrays have been constructed using designed peptide libraries with loop, a-helix, or P-strand structures. Here, we describe the overview of the reported deigned peptide arrays with loop and a-helix structures and the new results of those with beta-strand structures. Initially, several model peptides known to interact with model structured proteins were selected to establish the present strategy for high-throughput detection of proteins. The fluorescent probes and suitable scaffolds of peptides were examined for the effective detection of proteins. The detection methods were established in solution and in an immobilized manner using the model systems. In the case of a-helix peptide, the response of a peptide with fluorescent resonance energy tran, fer between two probes at both termini was several times higher than that of a peptide with a single probe. In the cases of peptides with other structures, however, proteins were effectively detectable even by the fluorescent change of one probe. Furthermore, structurally focused libraries consisting of a total of ca. 250 different peptides based on the model pcptides with secondary and/or tertiary structures were constructed with systematic replacement of residues. Using these libraries, various proteins were characterized effectively to give their own fluorescent "protein fingerprint" patterns. The resulting protein fingerprints correlated with the recognition properties of the proteins. These studies demonstrate that arrays with peptide libraries based on designed structures can be promising tools for detecting the target proteins. Designed synthetic peptides play roles as the capturing agents to be developed for practical protein chips. (C) 2004 Wiley Periodicals, Inc.

DOI： 10.1002/bip.10568

PubMed

Development of Peptide-Chips Focusing on a High Throughput Protein-Detection System Reviewed

Kiyoshi Nokihara, Takafumi Ohyama, Kenji Usui, Koichi. Yoneyama, Mizuki Takahashi, Hisakazu Mihara

Innovation and Perspectives in Solid Phase Synthesis & Combinatorial Libraries 83 - 88 2004

Peptide microarrays using structure-based peptide libraries for protein chips Reviewed

Kenji Usui, Mizuki Takahashi, Tetsunori Ojima, Masato Suzuki, Kiyoshi Nokihara, Eiichi Tamiya, Hisakazu Mihara

Peptide Revolution: Genomics, Proteomics & Therapeutics 258 - 259 2004

Joint Work

Publisher：AMER CHEMICAL SOC

Synthesis, Surface Modification and Multilayer Construction of Mixed Monolayer-Protected CdS Nanoparticles Reviewed

T. Tsuruoka, K. Akamatsu, H. Nawafune

Langmuir 20 11169 - 11174 2004

Joint Work

Microanalysis of Nucleic Acids Using the Limulus G Test

甲元一也

Anal. Sci ( 19 ) 1567 - 1568 2003.11

Single Work

アンチセンス治療に向けた多糖系遺伝子キャリアー

甲元一也

月刊BIOINDUSTRY 20 ( 10 ) 30 - 37 2003.11

Single Work

Polysaccharide-Polynucleotide Complexes Part 7. Hydrogen-ion and Salt Concentration Dependence of Complexation between Schizophyllan and Single-Stranded Homo RNAs

甲元一也

Bioorg. Chem ( 31 ) 216 - 226 2003.11

Single Work

Supramolecular structure of a sugar-appended organogelator explored with synchrotron X-ray small angle scattering

甲元一也

Langmuir, ( 19 ) 8211 - 8217 2003.11

Single Work

Polysaccharide-polynucleotide complexes Part 17. Solvent effects on conformational-transition of polydeoxyadenylic acid in the complex with schizophyllan

甲元一也

Polym. J. ( 35 ) 714 - 720 2003.11

Single Work

Polysaccharide-polynucleotide Complexes (15): Thermal Stability of Schizophyllan(SPG)/Poly(C) Triple Strands is Controllable by the a-Amino Acid Modification

甲元一也

Bioorg. Chem ( 31 ) 163 - 171 2003.11

Single Work

Structural Ttransition from Antiparallel to Parallel G-Quadruplex of d(G4T4G4) Induced by Ca2+.

三好大輔

Nucleic Acids Res ( 31 ) 1156 - 1163 2003.11

Single Work

バイオインフォマティクスを活用した未来型生体内・外センシングシステム

三好大輔

科学と工業, ( 77 ) 413 - 419 2003.11

Single Work

Novel biomaterials derived from deoxyribozyme and NAPzyme

三好大輔

Macromol. Chem. Phys. Macromol. Symp. ( 201 ) 245 - 252 2003.11

Single Work

Effect of Putrescine and PEG on a Structural Transition of DNA G-Quadruplex.

三好大輔

Nucleosides, Nucleotides, and Nucleic Acids ( 22 ) 1591 - 1594 2003.11

Single Work

Structural Competition Involving G-Quadruplex DNA and Its Complement.

三好大輔

Biochemistry ( 42 ) 11736 - 11744 2003.11

Single Work

Structural Polymorphism of Telomeric DNA Regulated by pH and Divalent Cation.

三好大輔

Nucleosides, Nucleotides, and Nucleic Acids ( 22 ) 203 - 221 2003.11

Single Work

Facile One-Pot Synthesis of Aromatic and Heteroaromatic Sulfonamides.

村嶋　貴之

J. Org. Chem., 2003.11

Single Work

J. Org. Chem., （学術雑誌、 2003）68, 8274-8276.

A new synthesis of functional dyes from 2-acenaphtho[1,2-c]pyrrole.

村嶋　貴之

Heterocycles, 2003.11

Single Work

Heterocycles, （学術雑誌、 2003） 61, 433-447 .

Utilization of proteases from Aspergillus species for peptide synthesis via the kinetically controlled approach.

村嶋　貴之

Biocatal. Biotrans., 2003.11

Single Work

Biocatal. Biotrans., （学術雑誌、 2003） 21, 93-100 .

Novel biomaterials derived from deoxyribozyme and napzyme.

中野　修一

Macromol. Symp. 201 245 - 252 2003.11

Single Work

An oligopeptide containing the C-terminal sequence of RNase A inhibits an RNA hydrolysis by RNase A.

S. Nakano, A. L. Cerrone, and P. C. Bevilacqua

First International Symposium on Biomolecular Chemistry 240 - 241 2003.11

Single Work

Large stacking stability of a base pair-mimic nucleotide on the DNA duplex.

中野　修一

Nucleic Acids Res. Supple. 3 79 - 80 2003.11

Single Work

Negative regulation of Lyn protein-tyrosine kinase by c-Cbl ubiquitin-protein ligase in Fc epsilon RI-mediated mast cell activation.

Kyo S, Sada K, Qu X, Maeno K, Miah SM, Kawauchi-Kamata K, Yamamura H

Genes to cells : devoted to molecular & cellular mechanisms 8 ( 10 ) 825 - 836 2003.10

Joint Work

PubMed

Structural competition involving G-quadruplex DNA and its complement. Reviewed

W. Li, D. Miyoshi, S. Nakano, and N. Sugimoto

Biochemistry, 42 11736 - 11744 2003.9

Single Work

DOI： 10.1021/bi034168j

“Pentacoordinated iron complexes with carboxamido nitrogens and thiolate sulfurs: As models for Fe-containing nitrile hydratase”

Satoshi Fujii, Takeshi Ogura, Shozo Kitamura and Hiroshi Sakai

J. Inorg. Biochem 96 134 2003.7

Joint Work

Authorship：Lead author

Adaptor protein 3BP2 is a potential ligand of Src homology 2 and 3 domains of Lyn protein-tyrosine kinase.

Maeno K, Sada K, Kyo S, Miah SM, Kawauchi-Kamata K, Qu X, Shi Y, Yamamura H

The Journal of biological chemistry 278 ( 27 ) 24912 - 24920 2003.7

Joint Work

DOI： 10.1074/jbc.M301201200

PubMed

An Oligopeptide containing the C-terminal sequence of RNase A has a potent RNase A binding property. Reviewed

S. Nakano and N. Sugimoto

J. Am. Chem. Soc. 125 8728 - 8729 2003.6

Single Work

DOI： 10.1021/ja034659r

Large stabilization of a DNA duplex by the deoxyadenosine derivatives tethering an aromatic hydrocarbon group. Reviewed

S. Nakano, Y. Uotani, S. Nakashima, Y. Anno, M. Fujii, and N. Sugimoto

J. Am. Chem. Soc. 125 8086 - 8087 2003.6

Single Work

DOI： 10.1021/ja034465b

核酸の長鎖ダングリングエンドと四重鎖の構造形成に関する熱力学的研究

三好大輔

2003.3

Single Work

Mechanistic chracterization of the HDV genomic ribozyme: Classifying the catalitic and structural metal ion sites within a multichannel mechanism. Reviewed

中野　修一

Biochemistry 42 2982 - 2994 2003.2

Single Work

DOI： 10.1021/bi026815x

Site-selective direct silver metallization on surface-modified polyimide layers

K. Akamatsu, S. Ikeda, H. Nawafune

Langmuir 19 10366 - 10371 2003

Joint Work

（学術雑誌、2003）19, 10366-10371

低温鉛フリーはんだ接合を目的としたスズー亜鉛共晶合金の電析および亜鉛の共析機構

縄舟秀美、中谷敏雄、赤松謙祐、内田衛、小幡恵吾

エレクトロニクス実装学会誌 6 222 - 227 2003

Joint Work

（学術雑誌、2003）6, 222-227

Fabrication and structural characterization of nanocomposites consisting of Ni nanoparticles dispersed in polyimide films

K. Akamatsu, K. Nakahashi, S. Ikeda, H. Nawafune

Eur. Phys. J. D 24 377 - 380 2003

Joint Work

（学術雑誌、2003）24, 377-380

Fabrication of copper film on aluminum nitride by novel electroless plating

H. Yanagimoto, K. Akamatsu, S. Deki, K. Gotoh

J. Electrochem. Soc. 150 260 - 265 2003

Joint Work

（学術雑誌、2003）150, 260-265

Covalent bond-mediated network fabrication of carboxylic anhydride-protected gold nanoparticles

K. Akamatsu, J. Hasegawa, H. Nawafune, H. Katayama, F. Ozawa

Trans. Mater. Res. Soc. Jpn. 28 927 - 930 2003

Joint Work

（学術雑誌、2003）28, 927-930

ULSI銅微細配線の形成を目的とした中性無電解銅めっきにおけるビアフィリング特性の改善

縄舟秀美、樋口晋吾、赤松謙祐、内田衛

表面技術 54 683 - 688 2003

Joint Work

（学術雑誌、2003）54, 683-688

Preparation and characterization of Au/Co nano-alloys

H. Nabika, M. Mizuhata, K. Kajinami, S. Deki, K. Akamatsu

J. Electroanal. Chem. 559 99 - 102 2003

Joint Work

（学術雑誌、2003）559, 99-102

Introduction of Ag and Ag₂S nanoparticles into nylon 6 film and fiber

Y. Gotoh, T. Kanno, Y. Fujimori, Y. Ohkoshi, M. Nagura, K. Akamatsu, S. Deki

Polymer Journal 35 960 - 964 2003

Joint Work

（学術雑誌、2003）35, 960-964

Synthesis of metallized polyimide films by surface chemical modification

S. Ikeda, K. Akamatsu, H. Nawafune

Trans. Mater. Res. Soc. Jpn 28 1267 - 1270 2003

Joint Work

.（学術雑誌、2003）28, 1267-1270

Ring-opening methathesis polymerization using alkenyl sulfides as chain-transfer agents: efficient routes to unsymmetrical poly(norbornen)-based macroinitiators bearing a terminal hydroxy group

H. Katayama, Y. Fukuse, Y. Nobuto, K. Akamatsu, F. Ozawa

Macromolecules 36 7020 - 7026 2003

Joint Work

（学術雑誌、2003）36, 7020-7026

NH₂-terminated poly(ethylene oxide) containing nano-sized NiO particles: synthesis, characterization and structural considerations

S. Deki, H. Yanagimoto, S. Hiraoka, K. Akamatsu, K. Gotoh

Chem. Mater. 15 4916 - 4922 2003

Joint Work

（学術雑誌、2003）15, 4916-4922

Surface modification-based synthesis and microstructural tuning of nanocomposite layers: monodispersed copper nanoparticles in polyimide resins

K. Akamatsu, S. Ikeda, H. Nawafune, S. Deki

Chem. Mater. 15 1488 - 2491 2003

Joint Work

（学術雑誌、2003）15, 1488-2491

Novel biomaterials derived from deoxyribozyme and NAPzyme Reviewed

Y. Okumoto, H. Fujiki, J. Kawakami, S. Nakashima, S. Nakano, T. Ohmichi, D. Miyoshi and N. Sugimoto

Macromol. Chem. Phys., Macromol. Symp. 201 245 - 252 2003

Important effects of non-Watson-Crick interactions and cation binding on nucleic acid structures.

中野　修一

Recent research development in nucleosides＆nucleotides （Transworld Research Networks）, Vol. 1, 59-80 2003

Single Work

Temparature dependence of themodynamic properties for DNA/DNA and RNA/DNA duplex formation. Reviewed

P. Wu, S. Nakano, and N. Sugimoto

Eur. J. Biochem. 269 2821 - 2830 2002.11

Single Work

DOI： 10.1046/j.1432-1033.2002.02970.x

Molecular Design of “Super” Hydrogelators: Understanding the Gelation process of Azobenzene-Based Sugar Derivatives in Water

甲元一也

Org. Lett., ( 4 ) 1423 - 1426 2002.11

Single Work

Chemical modification of curdlan: Characteristic complexation with polynucleotides by control of the molecular weight

甲元一也

Polym. Prep 43 ( 2 ) 721 - 723 2002.11

Single Work

Novel DNA-polysaccharide triple helix and its application to a gene carrier

甲元一也

Polym. Prep 43 ( 2 ) 691 - 692 2002.11

Single Work

Polysaccharide-polynucleotide Interaction (XI); Novel Separation System of RNAs by Using Schizophyllan-appended Column

甲元一也

Chem. Lett. 1240 - 1241 2002.11

Single Work

Polysaccharide-Polynucleotide Complexes Part 12: Enhanced affinity for various polynucleotide chains by site-specific chemical modification of schizophyllan

甲元一也

Perkin Trans. 1 2477 - 2484 2002.11

Single Work

Sol-gel phase transition induced by fiber-vesicle structural changes in sugar-based bolaamphiphiles

甲元一也

Perkin Trans 2 1930 - 1936 2002.11

Single Work

Gel formation properties of a uracil-appended cholesterol gelator and cooperative effects of the complementary nucleobases

甲元一也

Tetrahedron ( 58 ) 8863 - 8873 2002.11

Single Work

Polysaccharide-Polynucleotide Complexes Part 8. Cation Induced Complex Formation between Polyuridylic Acid and Schizophyllan

甲元一也

Biopolymers ( 65 ) 1 - 9 2002.11

Single Work

Long RNA Dangling End Has Large Energetic Contribution to Duplex Stability.

三好大輔

J. Am. Chem. Soc. ( 124 ) 10367 - 10372 2002.11

Single Work

シリーズ「生命を化学する」—モレキュラークラウディングとin cell Chemistry—

三好大輔

化学（化学同人） 57 ( 12 ) 50 - 56 2002.11

Single Work

超タンパク質材料—タンパク質活用最前線—

三好大輔

未来材料（NTS） ( 5 ) 29 - 35 2002.11

Single Work

Molecular Crowding Regulates the Structural Switch of the DNA G-quadruplex.

三好大輔

Biochemistry, ( 41 ) 15017 - 15024 2002.11

Single Work

Molecular Imprinting in Alcohols: utility of a pre-polymer based strategy for synthesizing stereoselective artificial receptor polymers in media hydrophilic　Anal.Chim.Acta

松井淳

（学術雑誌、 2002 ） 466, 11-15 11 - 15 2002.11

Single Work

コンビナトリアル・モレキュラーインプリンティングで目指す水系での分子認識　

松井淳

化学（学術雑誌、 2002 ）57, 33-38 33 - 38 2002.11

Single Work

Preparation of Novel Heteroisoindoles from Nitro Pyridines and Nitro Pyridones.

村嶋　貴之

Heterocycles, 2002.11

Single Work

Heterocycles, （学術雑誌、 2002） 58, 301-310 .

Aspergillus melleus protease-catalyzed peptide synthesis using the carbamoylmethyl ester as an acyl donor in 1,1,1,3,3,3-hexafluoro-2-propanol/N,N-dimethylformamide.

村嶋　貴之

Biotechnol. Lett., 2002.11

Single Work

Biotechnol. Lett., （学術雑誌、 2002） 24, 1945-1949.

Bacillus licheniformis protease-catalyzed peptides synthesis via the kinetically controlled approach using the carbamoylmethyl ester as an acyl donor in anhydrous acetonitrile.

村嶋　貴之

Lett. Pept. Sci., 2002.11

Single Work

Lett. Pept. Sci., （学術雑誌、 2002） 9, 173-177 .

Role of 2'-OH on Thermodynamic Stability and Structure of Chimeric Oligonucleotides

中野　修一

Nucleic Acids Res. Supple. 2 189 - 190 2002.11

Single Work

Long RNA Dangling end Has Large Energetic Contribution to Duplex Stability. Reviewed

T. Ohmichi, S. Nakano, D. Miyoshi, and N. Sugimoto

J. Am. Chem. Soc. 124 10367 - 10372 2002.8

Single Work

DOI： 10.1021/ja0255406

Hydrogen peroxide activates IkappaB kinases through phosphorylation of serine residues in the activation loops.

Kamata H, Manabe T, Oka Si, Kamata K, Hirata H

FEBS letters 519 ( 1-3 ) 231 - 237 2002.5

Joint Work

PubMed

“Comparison of Nitric Oxide Production in Response to Carbachol Between Macrovascular and Microvascular Cardiac Endothelial Cells” Reviewed

Takehiko Miyashita, Yasuchika Takeishi, Hiroki Takahashi, Takuya Miyamoto, Satoshi Fujii, Tetsuhiko Yoshimura, Hitonobu Tomoike, Shuichi Kato, and Isao Kubota

Circulation Journal, 66 ( 5 ) 511 - 515 2002.4

Joint Work

「アルツハイマー病とNO・金属イオンの化学」

藤井　敏司,杉本直己

化学 57 ( 4 ) 43 - 47 2002.4

Joint Work

Authorship：Lead author

「狂牛病・プリオン病と金属イオンの化学」

藤井　敏司,杉本直己

化学 57 ( 3 ) 30 - 34 2002.3

Joint Work

Authorship：Lead author

"In Vivo Nitric Oxide Transfer of a Physiological NO Carrier, Dinitrosyl Dithiolato Iron Complex, to Target Complex" Reviewed

Takaharu Ueno, Yasuhiro Suzuki, Satoshi Fujii, Anatoly F. Vanin, and Tetsuhiko Yoshimura

Biochem. Pharmacol. 63 ( 3 ) 485 - 493 2002.2

Joint Work

Ciona intestinalis cDNA projects: expressed sequence tag analyses and gene expression profiles during embryogenesis

西方敬人

Gene ( 287 ) 83 - 96 2002

Single Work

Novel G-protein-coupled receptor gene expressed specifically in the entire neural tube of the ascidian Ciona intestinalis

西方敬人

Development, Genes and Evolution ( 212 ) 447 - 451 2002

Single Work

Highly reactive intermediate-functionalized gold clusters: Synthesis and immobilization on silica supports through amide-forming coupling

K. Akamatsu, J. Hasegawa, H. Nawafune, H. Katayama, F. Ozawa

J. Mater. Chem. 12 2862 - 2865 2002

Joint Work

（学術雑誌、2002）12, 2862-2865

鉛フリーはんだ接合を目的としたスルホコハク酸浴からのSn-0.7Cu-0.3Ag３元合金の電析

縄舟秀美、芝一博、赤松謙祐、水本省三、内田衛、小幡恵吾

エレクトロニクス実装学会誌 5 146 - 151 2002

Joint Work

（学術雑誌、2002）5, 146-151

フリップチップ実装を目的としたスルホコハク酸浴からのスズ−インジウム共晶合金の電析

縄舟秀美、中谷敏雄、赤松謙祐、水本省三、小幡恵吾、内田衛

エレクトロニクス実装学会誌 5 349 - 352 2002

Joint Work

（学術雑誌、2002）5, 349-352

Fabrication and characterization of PAN-derived carbon thin films containing Au nanoparticles

S. Deki, H. Nabika, K. Akamatsu, M. Mizuhata, A. Kajinami, S. Tomita, M. Fujii, S. Hayashi

Thin Solid Films 408 59 - 63 2002

Joint Work

（学術雑誌、2002）408, 59-63

Novel copper electroless plating process on ceramic substrate using copper oxide

H. Yanagimoto, K. Akamatsu, S. Deki, K. Gotoh

Electrochem. Solid State Lett. 5 C87 - C89 2002

Joint Work

（学術雑誌、2002）5, C87-C89

Growth of metal oxide thin films from aqueous solution by liquid phase deposition, LPD, method

S. Deki, N. Yoshida, Y. Hiroe, K. Akamatsu, M. Mizuhata, A. Kajinami

Solid State Ionics 151 1 - 9 2002

Joint Work

（学術雑誌、2002）151, 1-9

Microstructure and electron transport properties of Au_xCo_1-x nano-alloys embedded in polyacrylonitrile thin films

H. Nabika, K. Akamatsu, M. Mizuhata, A. Kajinami, S. Deki

J. Mater. Chem. 12 2408 - 2411 2002

Joint Work

（学術雑誌、2002）12, 2408-2411

Effect of composition and structure of gold/copper bimetallic nanoparticles on dispersion in polymer thin films

K. Akamatsu, T. Kawamura, H. Nabika, S. Deki, T. Strunskus, F. Faupel

J. Mater. Chem. 12 3610 - 3614 2002

Joint Work

（学術雑誌、2002）12, 3610-3614

Synergistic effects induced by cycloprodigiosin hydrochloride and epirubicin on human breast cancer cellsYamamoto D Reviewed

Tanaka K, Nakai K, Baden T, Inoue K, Yamamoto C, Takemoto H, Kamata K, Hirata H, Morikawa S, Inubushi T, Hioki K

Breast Cancer Research and Treatment 72 2002

分子転写法による新素材の開発 –生体分子を超える分子認識材料開発を目指して-

甲元一也

未来材料 1 ( 5 ) 35 - 41 2001.11

Single Work

Application of schizophyllan as a novel gene carrier

甲元一也

Nucleic Acids Res. Supplement ( 1 ) 223 - 224 2001.11

Single Work

A novel technique to specifically separate RNAs by Schizophyllan

甲元一也

Nucleic Acids Res. Supplement ( 1 ) 283 - 284 2001.11

Single Work

Chemical Modification of Curdlan to Induce an Interaction with Poly(C)

甲元一也

Chem. Lett 908 - 909 2001.11

Single Work

Chemical modification of schizophyllan by introduction of a cationic charge into the side chain which enhances the thermal stability of schizophyllan/poly(C) complexes

甲元一也

Chem. Commun 1962 - 1963 2001.11

Single Work

Organization of Nucleosides Supported by Boronic-Acid-Appended poly(L-Lysine): Creation of a Novel RNA Mimic

甲元一也

Bull. Chem. Soc. Jpn ( 74 ) 1311 - 1317 2001.11

Single Work

Polysaccharide-Polynucleotide Complexes (IV): Anti-hydrolysis effect of the schizophyllan/poly(C) complex and the complex dissociation induced by amines

甲元一也

Bioorg. Chem., ( 29 ) 178 - 185 2001.11

Single Work

Thermally induced conformational–transition of polydeoxyadenosine in the complex with schizophyllan and the base-length dependence of its stability

甲元一也

Chem. Commun. 429 - 430 2001.11

Single Work

A Stable DNA tetraloop and Its Structural Tolerance for Modification.

三好大輔

Chem. Lett 258 - 259 2001.11

Single Work

Structural transition of d(G4T4G4) from antiparallel to parallel G-quartet induced by divalent cations

三好大輔

Nucleic Acids Res. Supple ( 1 ) 259 - 260 2001.11

Single Work

Effect of Divalent Cations on Antiparallel G-Quartet Structure of d(G4T4G4).

三好大輔

FEBS Lett ( 496 ) 128 - 133 2001.11

Single Work

Molecularly Imprinted Polymers with Metalloporphyrin-Based Molecular Recognition Sites Coassembled with Methacrylic Acid　Anal.Chem.

松井淳

（学術雑誌、 2001 ） 73, 3869-3874 3869 - 3874 2001.11

Single Work

Molecularly Imprinted Polymer Library on a Microtiter Plate: High-throughput Synthesis and Assessment of Cinchona Alkaloid-imprinted Polymer Receptors　Instrumentation Sci. & Technologyvol.　

松井淳

（学術雑誌、2001）29, 1-9 1 - 9 2001.11

Single Work

Combinatorial Molecular Imprinting for Formation of Atrazine Decomposing Polymers　Chem.Lett.

松井淳

（学術雑誌、 2001 ） 530-531 530 - 531 2001.11

Single Work

Synthesis and optical properties of a new class of pyrromethene-BF2-complexes fised with rigid bicyclo rings and benzo derivatives.

村嶋　貴之

Tetrahedron Lett., 2001.11

Single Work

Tetrahedron Lett., （学術雑誌、 2001） 6711-6713.

Synthesis of benzoporphyrins functionalized with octaester groups.

村嶋　貴之

Tetrahedron Lett., 2001.11

Single Work

Tetrahedron Lett.,（学術雑誌、2001） 42, 45-47.

Synthesis and properties of polypyrrole annelated with bicyclo[2.2.2]octene units.

村嶋　貴之

Tetrahedron Lett., 2001.11

Single Work

Tetrahedron Lett.,（学術雑誌、2001） 42, 707-710.

Preparation of meso-unsubstituted porphyrins substituted with mono- and tetraformyl and other electron-withdrawing groups.

村嶋　貴之

Heterocycles, 2001.11

Single Work

Heterocycles,（学術雑誌、2001） 54, 705-720.

Synthesis of b,b'-(phenylene)diporphyrins.

村嶋　貴之

Synthesis, 2001.11

Single Work

Synthesis, （学術雑誌、2001） 399-402.

Synthesis of oligo(thienylenevinylenes) substituted with alkoxy groups.

村嶋　貴之

Heteroatom Chemistry, 2001.11

Single Work

Heteroatom Chemistry, （学術雑誌、 2001） 12, 414-417.

“Spin trapping agent, phenyl N-tert-butylnitrone, reduces nitric oxide production in the rat brain during experimental meningitis”

藤井　敏司

Free Radic Res ( 35 ) 583 - 591 2001.11

Single Work

Proton Inventory of the Genomic HDV Ribozyme in Mg2+-Containing Solutions. Reviewed

S. Nakano and P. C. Bevilacqua

J. Am. Chem. Soc. 123 11333 - 11334 2001.10

Single Work

DOI： 10.1021/ja0166850

Mechanistic Characterization of the HDV Genomic Ribozyme: Assessing the Catalytic and Structural Contributions of Divalent Metal Ions within a Multichannel Reaction Mechanism. Reviewed

S. Nakano, D. J. Proctor, and P. C. Bevilacqua

Biochemistry 40 12022 - 12038 2001.9

Single Work

DOI： 10.1021/bi011253n

Stabilization Factors Affecting Duplex Formation fo Peptide Nucleic Acid with DNA. Reviewed

N. Sugimoto, N. Satoh, K. Yasuda, and S. Nakano

Biochemistry 40 8444 - 8451 2001.6

Single Work

DOI： 10.1021/bi010480m

A Stable DNA Tetraloop and Its Structural Tolerance for Modification

KAWAKAMI Junji, YONEYAMA Mari, MIYOSHI Daisuke, SUGIMOTO Naoki

Chemistry Letters 2001 ( 3 ) 258 - 259 2001.3

Publisher：The Chemical Society of Japan

Structural features of a stable hairpin DNA with a tetraloop, dAGGC(TTCG)GCCT (CORE), and its six abasic mutants were investigated by the melting behavior of the UV absorption and circular dichroism spectra. The CORE and an abasic mutant at the second position of the loop exhibit considerable thermodynamic stability and have similar structural features suggesting the same folding with an identical base orientation of the two hairpin DNAs except for the second base in the loop.

DOI： 10.1246/cl.2001.258

CiNii Article

CiNii Books

Maternal genetic information stored in fertilized eggs of the ascidian, Halocynthia roretzi.

西方敬人

in Biology of Ascidian Springer-Verlag 165 - 177 2001

Single Work

Profiles of maternally expressed genes in fertilized eggs of Ciona intestinalis

西方敬人

Developmental Biology ( 238 ) 315 - 331 2001

Single Work

Large-scale cDNA analysis of the maternal genetic information in the egg of Halocynthia roretzi for a gene expression catalog of ascidian development.

西方敬人

Development ( 128 ) 2555 - 2567 2001

Single Work

Utilization of ribozymes for loss-of-function analyses of the early development of the ascidian Ciona intestinalis.

西方敬人

Mem. Konan Univ., Sci. Ser. ( 48 ) 11 - 20 2001

Single Work

Functional analysis of Ciona intestinalis Y-box protein.

西方敬人

in Biology of Ascidian Springer-Verlag 186 - 192 2001

Single Work

RNA-binding proteins in ascidian development.

西方敬人

RNA-binding proteins in ascidian development. 178 - 185 2001

Single Work

Utilization of ribozymes for loss-of-function analyses of the early development of the ascidian Ciona intestinalis.

Katano, M., Yamada, A., Tanaka, K. J., Murakami, A., Taira, K., Kawakami, J. Sugimoto, N., and Nishikata, T.

Mem. Konan Univ. Sci. Ser. 48 11 - 20 2001

Single Work

Improved antigen detection on Western-blots Reviewed

Matsugu, H., Nishikata, T.

Technical Tips Online 6 ( 1 ) 34 - 36 2001

Single Work

Publisher：Elsevier

DOI： 10.1016/S1366-2120(08)70163-6

Direct photochemical formation of Cu patterns on surface modified polyimide resin

S. Ikeda, K. Akamatsu, H. Nawafune

J. Mater. Chem. 11 2919 - 1921 2001

Joint Work

（学術雑誌、2001）11, 2919-1921

Formation of copper nanoparticles in surface modified polyimide resin

K. Akamatsu, S. Ikeda, H. Nawafune, S. Dek

Proc. of the 7th International Symposium on Advanced Physical Fields 2001 392 - 395 2001

Joint Work

（学術雑誌、2001）, 392-395

Single-electron tunneling effects in nylon 11 thin films containing nanoparticles

M. Fujii, Y. Inoue, S. Hayashi, K. Akamatsu, S. Deki

Jpn. J. Appl. Phys. 40 1911 - 1914 2001

Joint Work

（学術雑誌、2001）40, 1911-1914

Preparation and characterization of metal nanoparticles dispersed in polyacrylonitrile thin film

S. Deki. H. Nabika, K. Akamatsu, M. Mizuhata, A. Kajinami

Scripta Mater. 44 1879 - 1882 2001

Joint Work

（学術雑誌、2001）44, 1879-1882

Formation process and characterization of copper oxide nanoparticles dispersed in polymer thin films

K. Akamatsu, S. Deki

Scripta Mater. 44 2149 - 2152 2001

Joint Work

（学術雑誌、2001）44, 2149-2152

ポリイミド樹脂の表面改質および熱圧着を利用する銅のDirect Metallization

池田慎吾、赤松謙祐、縄舟秀美、水本省三、清田優

エレクトロニクス実装学会誌 4 603 - 606 2001

Joint Work

（学術雑誌、2001）4, 603-606

添加剤の予備吸着を利用する銅電析プロセスによるULSI微細配線の形成

縄舟秀美、粟野まいこ、赤松謙祐、水本省三、内田衛、細田勉

表面技術 53 59 - 64 2001

Joint Work

（学術雑誌、2001）53, 59-64

Synthesis of titanium carbide from a composite of TiO₂ nanoparticles/methyl cellulose by carbothermal reduction

Y. Gotoh, K. Fujimura, M. Koike, Y. Ohkoshi, M. Nagura, K. Akamatsu, S. Deki

Mater. Res. Bull. 36 2263 - 2275 2001

Joint Work

（学術雑誌、2001）36, 2263-2275

Preparation of Cu₂O nanoparticles dispersed in NH2-terminated polyethylene oxide matrix

H. Yanagimoto, K. Akamatsu, K. Goto, S. Deki

Eur. Phys. J. D 16 313 - 316 2001

Joint Work

（学術雑誌、2001）16, 313-316

Synthesis and microstructure of metal oxide thin films containing metal nanoparticles by liquid phase deposition (LPD) method

S. Deki, Hin Yu Yu Ko, T. Fujita, K. Akamatsu, M. Mizuhata, A. Kajinami

Eur. Phys. J. D 16 325 - 328 2001

Joint Work

（学術雑誌、2001）16, 325-328

Composition dependence on dispersion of Au/Cu bimetallic nanoparticles in nylon 11 thin films

K. Akamatsu, T. Kawamura, S. Deki

Eur. Phys. J. D 16 305 - 308 2001

Joint Work

（学術雑誌、2001）16, 325-328

Novel fabrication method for Si_1-xTi_xO₂ thin films with garaded composition profiles by liquid phase deposition

S. Deki, S. Iizuka, K. Akamatsu, M. Mizuhata, A. Kajinami

J. Mater. Chem. 11 984 - 986 2001

Joint Work

（学術雑誌、2001）11, 984-986

Synthesis and characterization of Cu₂O nanoparticles dispersed in NH₂-terminated poly(ethylene oxide)

H. Yanagimoto, K. Akamatsu, K. Gotoh, S. Deki

J. Mater. Chem. 11 2387 - 2389 2001

Joint Work

（学術雑誌、2001）11, 2387-2389

Utilization of ribozymes for loss-of-function analyses of the early development of the ascidian Ciona intestinalis

M. Katano, A. Yamada, K. J. Tanaka, A. Murakami, K. Taira, J. Kawakami, N. Sugimoto and T. Nishikata

Mem. Konan Univ., Sci. Eng. Ser. 48 11 - 20 2001

Thermodynamic stability of base pairs between 2-hydroxyadenine and incoming nucleotides as a determinant of nucleotide incorporation specificity during replication Reviewed

J. Kawakami, H. Kamiya, K. Yasuda, H. Fujiki, H. Kasai and N. Sugimoto

Nucleic Acids Research 29 3289 - 3296 2001

Cycloprodigiosin hydrocloride suppresses tumor necrosis factor (TNF) alpha-induced transcriptional activation by NF-kappaB Reviewed

Kamata K, Okamoto S, Oka S, Kamata H, Yagisawa H, Hirata H

FEBS Letters 507 2001

Authorship：Lead author

Cycloprodigiosin hydrochloride, a H+/Cl- symporter, induces apoptosis in human colon cancer cell lines in vitro

Yamamoto C, Takemoto H, Kuno K, Yamamoto D, Nakai K, Baden T, Kamata K, Hirata H, Watanabe T, Inoue K

Oncology Reports 8 2001

Colorimetric Sugar Sensing Method Useful in “Neutral” Aqueous Media

甲元一也

Chem. Lett., 856 - 857 2000.11

Single Work

TEM and SEM observations of super-structures constructed in organogel systems from a combination of boronic-acid-appended bola-amphiphiles with chiral diols

甲元一也

Nanotechnology, ( 12 ) 25 - 31 2000.11

Single Work

Polysaccharide-Polynucleotide Complex (III): A novel interaction between the b-1,3-Glucan Family and the Single-Strand RNA: Poly(C)

甲元一也

Chem. Lett 1242 - 1243 2000.11

Single Work

The First Example of Positive Allosterism in an Aqueous Saccharide-Binding System Designed on a Ce(IV) Bis(porphyrinate) Double Decker Scaffold

甲元一也

Tetrahedron ( 56 ) 4717 - 4723 2000.11

Single Work

Development of Small Peptides Recognizing a Monosaccharide by Combinatorial Chemistry.

三好大輔

Chem. Commun 2295 - 2296 2000.11

Single Work

ゲノム化学は今3　SNPs解析と次世代の生命化学　—「ニューセントラルドグマ」の構築に向けて.

三好大輔

化学（化学同人） ( 55 ) 20 - 25 2000.11

Single Work

ゲノム化学は今2　DNAチップとバイオインフォマティクス　—遺伝子の定量的解析に向けて.

三好大輔

化学（化学同人） ( 55 ) 14 - 18 2000.11

Single Work

Molecularly Imprinted 9-Ethyladenine Receptor Having Porphyrin-based Recognition Center　J. Am. Chem. Soc

松井淳

.（学術雑誌、2000）122, 5218-5219 5218 - 5219 2000.11

Single Work

Solid - phase Extraction with a Dibutylmelamine-imprinted Polymer as Triazine Herbicide - selective Sorbent

松井淳

J. Chromatogr., A（学術雑誌、2000）889, 25-31 25 - 31 2000.11

Single Work

Miniaturized Molecularly Imprinted Contiunous Polymer Rods　J. High Resolution Chromatogr.

松井淳

J. High Resolution Chromatogr.（学術雑誌、2000）23, 44-46 44 - 46 2000.11

Single Work

Molecularly Imprinted Fluorescent-shift Receptors Prepared Using 2-(Trifluoromethyl)acrylic Acid　Anal. Chem.

松井淳

（学術雑誌、2000）72, 3286-3290 3286 - 3290 2000.11

Single Work

Atrazine-selective Polymers Prepared by Molecular Imprinting of Trialkylmelamines as Dummy Template Species of Atrazine　Anal. Chem.

松井淳

（学術雑誌、2000）72, 1810-1813 1810 - 1813 2000.11

Single Work

Synthesis and coplanarity-dependent HOMO-LUMO separation of conjugated dimers.

村嶋　貴之

J. Chem. Soc., Perkin Trans. 1, 2000.11

Single Work

J. Chem. Soc., Perkin Trans. 1,（学術雑誌、2000） 2671-2675.

A new synthesis of benzoporphyrins using 4,7-dihydro-4,7-ethano-2H-isoindole as an isoindole equivalent.

村嶋　貴之

Heterocycles, 2000.11

Single Work

Heterocycles,（学術雑誌、2000） 52, 399-411.

A new synthesis of [2,3]naphthoporphyrins.

村嶋　貴之

Chem. Commun., 2000.11

Single Work

Chem. Commun.,（学術雑誌、2000） 893-894.

Synthesis and structures of pyrroles fused with rigid bicyclic ring systems at b-positions.

村嶋　貴之

J. Chem. Soc., Perkin Trans. 1, 2000.11

Single Work

J. Chem. Soc., Perkin Trans. 1,（学術雑誌、2000） 4347-4355.

Synthesis and X䀭ray structure of stable 2H-isoindoles.

村嶋　貴之

J. Chem. Soc., Perkin Trans. 1 2000.11

Single Work

J. Chem. Soc., Perkin Trans. 1,（学術雑誌、2000） 995-998.

“Nitric Oxide Enhances Expression and Shedding of Tumor Necrosis Factor Receptor I (p55) in Endothelial Cells”

藤井　敏司

Arterioscler Thromb Vasc Biol. ( 20 ) 1506 - 1511 2000.11

Single Work

“Detection and Imaging of Endogenously Produced Nitric Oxide with Electron Paramagnetic Resonance Spectroscopy”

藤井　敏司

Antiox. Redox Signal ( 2 ) 879 - 901 2000.11

Single Work

"A New Trend in Iron-Dithiocarbamate Complexes: As an Endogenous NO Trapping Agent."

藤井　敏司

Coord. Chem. Rev ( 198 ) 89 - 99 2000.11

Single Work

"Reaction of Nitric Oxide with the Iron(III) Complex of N-(dithiocarboxy)sarcosine: A New Type of Reductive Nitrosylation Involving Iron(IV) as an Intermediate”

藤井　敏司

J. Chem. Soc. Dalton Trans 3310 - 3315 2000.11

Single Work

Thermodynamics-Structure Relationship of Single Mismatches in RNA/DNA Duplexes. Reviewed

N. Sugimoto, M. Nakano, and S. Nakano

Biochemistry 39 11270 - 11281 2000.8

Single Work

DOI： 10.1021/bi000819p

A Role for Upstream RNA Structure in Facilitating the Catalytic Fold of the Genomic Hepatitis Delta Virus Ribozyme. Reviewed

D. M. Chadalavada, S. M. Knudsen, S. Nakano, and P. C. Bevilacqua

J. Mol. Biol. 301 349 - 367 2000.3

Single Work

DOI： 10.1006/jmbi.2000.3953

Expression patterns of musashi homologs of the ascidians, Halocynthia roretzi and Ciona intestinalis Reviewed

T Kawashima, AR Murakami, M Ogasawara, KJ Tanaka, R Isoda, Y Sasakura, T Nishikata, H Okano, KW Makabe

DEVELOPMENT GENES AND EVOLUTION 210 ( 3 ) 162 - 165 2000.3

Joint Work

Publisher：SPRINGER VERLAG

The gene family encoding RNA-binding proteins includes important regulators involved in the neurogenesis in both protostomes and deuterostomes. We isolated cDNAs of the ascidian homolog of one of the RNA-binding proteins, MUSASHI, from Halocynthia roretzi and Ciona intestinalis. The predicted amino acid sequences contained two RNA-recognition and RNA-binding motifs in the N-terminus and an ascidian-specific YG-rich domain in the C-terminus. Maternal transcripts of musashi were ubiquitous in early cleavage-stage embryos. Ascidian musashi had three domains of zygotic expression: the brain, nerve cord, and mesenchyma. The temporal order of the onset in these domains was highly divergent between the two species of ascidian examined.

General Acid-Base Catalysis in the Mechanism of a Hepatitis Delta Virus Ribozyme. Reviewed

S. Nakano, D. M. Chadalavada, and P. C. Bevilacqua

Science 287 1493 - 1497 2000.2

Single Work

DOI： 10.1126/science.287.5457.1493

Expression patterns of musashi homologus of the ascidians, Halocynthia roretzi and Ciona intestinalis.

西方敬人

Devl. Genes Evol ( 210 ) 162 - 165 2000

Single Work

Zoospore-specific antigen as a useful marker for molecular analysis of net formation in Hydrodictyon reticulatum (Chlorococcales, Chlorophyceae).

西方敬人

Phycol. Res ( 48 ) 143 - 148 2000

Single Work

Improved antigen detection on Western-blots.

西方敬人

Tech. Tips Online http://www.elsevier.com/locate/tto/ T02043 2000

Single Work

The transcript coding for an RNA-binding protein is localized in the anterior side of the ascidian 2-cell stage embryo.

西方敬人

Devl. Gene Evol. ( 210 ) 464 - 466 2000

Single Work

An ascidian glycine-rich RNA-binding protein is not induced by temperature-stress but is expressed under a genetic program during embryogenesis.

西方敬人

Gene ( 243 ) 207 - 214 2000

Single Work

Zoospore-specific antigen as a useful marker for molecular analysis of net formation in Hydrodictyon reticulatum(Chlorococcales, Chlorophyceae) Reviewed

Hatano, K., Yamamoto, M., Yamada, Y., Nishikata, T.

Phycol Res 48 143 - 148 2000

Joint Work

DOI： 10.1046/j.1440-1835.2000.00196.x

The transcript coding for an RNA-binding protein is localized in the anterior side of the ascidian 2-cell stage embryo Reviewed

Tanaka, K. J., Kawamura, H., Nishikata, T.

Devl. Gene Evol. 210 464 - 466 2000

Joint Work

Expression patterns of musashi homologus of the ascidians, Halocynthia roretzi and Ciona intestinalis Reviewed

Kawashima, T., Murakami, A. R., Ogasawara, M., Tanaka, K. J., Isoda, R., Sasakura, Y., Nishikata, T., Okano, H., Makabe, K. W.

Devl. Genes Evol. 210 162 - 165 2000

Joint Work

An ascidian glycine-rich RNA-binding protein is not induced by temperature-stress but is expressed under a genetic program during embryogenesis Reviewed

Tanaka, K. J., Kawamura, H., Matsugu, H., Nishikata, T.

Gene 243 207 - 214 2000

Joint Work

DOI： 10.1016/S0378-1119(99)00567-3

In situ spectroscopic and microscopic study on dispersion of Ag nanoparticles in polymer thin films

K. Akamatsu, N. Tsuboi, Y. Hatakenaka, S. Deki

J. Phys. Chem. B 104 10168 - 10173 2000

Joint Work

（学術雑誌、2000）104, 10168-10173

Au超微粒子を埋め込んだナイロン11蒸着膜の構造と物性

赤松謙祐、出来成人、井上翼、藤井稔、林真至

信学技報 OME 200-99 1 - 6 2000

Joint Work

（学術雑誌、2000）OME 200-99, 1-6

Preparation and characterization of polymer thin films containing silver and silver sulfide nanoparticles

K. Akamatsu, S. Takei, M. Mizuhata, A. Kajinami, S. Deki, S. Takeoka, M. Fujii, S. Hayashi, K. Yamamoto

Thin Solid Films 359 55 - 60 2000

Joint Work

（学術雑誌、2000）359, 55-60

Single-electron tunneling effects in thin nylon 11 films containing gold nanoparticles

Y. Inoue, M. Fujii, M. Inata, S. Hayashi, K. Yamamoto, K. Akamatsu, S. Deki

Thin Solid Films 372 169 - 172 2000

Joint Work

（学術雑誌、2000）372, 169-172

Formation of Co filled carbon nanocapsules by metal-template graphitization of diamond nanoparticles

S. Tomita, M. Hikita, M. Fujii, S. Hayashi, K. Akamatsu, S. Deki, H. Yasuda

J. Appl. Phys. 88 5452 - 5456 2000

Joint Work

（学術雑誌、2000）88, 5452-5456

Preparation and structure of copper nanoparticles/poly(acrylic acid) composite films

Y. Gotoh, R. Igarashi, Y. Ohkoshi, M. Nagura, K. Akamatsu, S. Deki

J. Mater. Chem. 10 2548 - 2552 2000

Joint Work

（学術雑誌、2000）10, 2548-2552

In vitro selection of aptamers that act with Zn2+ Reviewed

J. Kawakami, H. Imanaka, Y. Yokota and N. Sugimoto

J. Inorg. Biochem. 82 197 - 206 2000

Analysis for peptide binding to porphyrin using surface plasmon resonance

C. Nakamura, Y. Inuyama, K. Shirai, S. Nakano, N. Sugimoto, and J. Miyake

Synthetic Metals 117 127 - 129 2000

Joint Work

DOI： 10.1016/S0379-6779(00)00552-X

"A New Trend in Iron-Dithiocarbamate Complexes : As an Endogenous NO Trapping Agent. " Invited Reviewed

FUJII Satoshi

Coord. Chem. Rev. 198 89 - 99 2000

Single Work

Cycloprodigiosin hydrochloride, H(+)/CL(-) symporter, induces apoptosis and differentiation in HL-60 cells Reviewed

Yamamoto D, Uemura Y, Tanaka K, Nakai K, Yamamoto C, Takemoto H, Kamata K, Hirata H, Hioki K

International Journal of Cancer 88 2000

N-acetylcysteine suppresses TNF-induced NF-kappaB activation through inhibition of IkappaB kinases Reviewed

Oka S, Kamata H, Kamata K, Yagisawa H, Hirata H

FEBS Letters 2000

Demonstration of Cl- requirement for inhibition of vacuolar acidification by cycloprodigiosin in situ Reviewed

Nakayasu T, Kawauchi K, Hirata H, Shimmen T

Plant & Cell Physiol 41 2000

A Novel Screening Method for Combinatorial Chemistry for Low Affinity Interactions.

三好大輔

Chem. Commun. 677 - 678 1999.11

Single Work

Selection of peptides that recognize tryptophan by combinatorial chemistry

三好大輔

Peptide Science 281 - 284 1999.11

Single Work

a-b Structural Transition of Short Oligopeptides by Water/Organic Solvent Titration.

三好大輔

Chem. Lett 637 - 638 1999.11

Single Work

"四塩化炭素肝障害にて発生した一酸化窒素のジチオカルボキシサルコシン鉄錯体による捕捉"

藤井　敏司

磁気共鳴と医学(日本医学館) ( 10 ) 16 - 20 1999.11

Single Work

"In vivo Distribution and Behavior of Paramagnetic Dinitrosyl Dithiolato Iron Complex in the Abdomen of Mouse"

藤井　敏司

Free Radic. Res. ( 31 ) 525 - 534 1999.11

Single Work

"Direct Evidence of In Vivo Nitric Oxide Production and Inducible Nitric Oxide Synthase mRNA Expression in the Rat Brain of Living Rat During Experimental Meningitis"

藤井　敏司

J. Cereb. Blood Flow Metab ( 19 ) 1175 - 1178 1999.11

Single Work

"内因性NOドナー(NOチオレート鉄錯体)のマウス腹部における分布,動態に関する研究"

藤井　敏司

G.I.Research ( 7 ) 85 1999.11

Single Work

"内因性一酸化窒素のジチオカルバメート誘導体鉄錯体による捕捉検出～検出法の基礎的検討～"

藤井　敏司

磁気共鳴と医学(日本医学館) ( 10 ) 21 - 25 1999.11

Single Work

Nucleic Acid Duplex Stability : Influence of Base Composition on Cation Effects. Reviewed

S. Nakano, M. Fujimoto, H. Hara, and N. Sugimoto

Nucleic Acids Res. 27 2957 - 2965 1999.7

Single Work

DOI： 10.1093/nar/27.14.2957

Generation and characterization of a monoclonal antibody, mH1, raised against mitotic HeLa cells.

西方敬人

Devl. Growth Differ ( 41 ) 381 - 389 1999

Single Work

A non-radioactive gel shift protocol enables recovery of RNA-binding proteins.

西方敬人

Tech. Tips Online http://www.elsevier.com/locate/tto/T01794 1999

Single Work

Chracterization of MTR1: A novel protein correlated with the myoplasmic movement during ascidian ooplasmic segregation.

西方敬人

Mem. Konan Univ., Sci. Ser. ( 46 ) 25 - 33 1999

Single Work

The CAB (Centrosome-Attracting Body), microtubule system and posterior egg cytoplasm are involved in positioning of cleavage planes in the ascidian embryo.

西方敬人

Devl. Biol ( 209 ) 72 - 85 1999

Single Work

The expression of the protochordate homologue of the proteasome regulatory subunit Rpn12 is transcriptionally , and post-translationally regulated during cleavage stage.

西方敬人

Zool. Sci. ( 16 ) 125 - 129 1999

Single Work

Interactions between cytoskeletal components during ooplasmic segregation in the myoplasm of ascidian eggs.

西方敬人

Devl. Growth Differ. ( 41 ) 265 - 272 1999

Single Work

The expression of the protochordate homologue of the proteasome regulatory subunit Rpn12 is transcriptionally and post-translationally regulated during cleavage stage.(共著) Reviewed

NISHIKATA Takahito

Zool. Sci. 16, 125-129 1999

Single Work

DOI： 10.2108/zsj.16.125

Generation and characterization of a monoclonal antibody, mH1, raised against mitotic HeLa cells.(共著) Reviewed

NISHIKATA Takahito

Devl. Growth Differ. 41, 381-389 1999

Single Work

DOI： 10.1046/j.1440-169X.1999.00438.x

Interactions between cytoskeletal components during ooplasmic segregation in the myoplasm of ascidian eggs.(共著) Reviewed

NISHIKATA Takahito

Devl. Growth Differ. 41, 265-272 1999

Single Work

DOI： 10.1046/j.1440-169X.1999.413433.x

The CAB(Centrosome-Attracting Body), microtubule system, and posterior egg cytoplasm are involved in positioning of cleavage planes in the ascidian embryo.(共著) Reviewed

NISHIKATA Takahito

Devl. Biol. 209, 72-85 1999

Single Work

DOI： 10.1006/dbio.1999.9244

A non-radioactive gel shift protocol enables recovery of RNA-binding proteins Reviewed

Tanaka, K. J., Nishikata, T.

Tech. Tips Online 4 ( 1 ) 70 - 72 1999

Joint Work

Publisher：Elsevier

DOI： 10.1016/S1366-2120(08)70143-0

Characterization of MTR1: A novel protein correlated with the myoplasmic movement during ascidian ooplasmic segregation.

Nakamori, R., Murakami, A., and Nishikata, T.

Memoirs of Konan University, Science and Engineering Series. 46 25 - 33 1999

Single Work

Characterization of MTR1 : A novel protein correlated with the myoplasmic movement during ascidian ooplasmic segregation.(共著)

NISHIKATA Takahito

Mem. Konan Univ. Sci. Ser 46, 25-33 1999

Single Work

TEM investigation and electron diffraction study on dispersion of gold nanoparticles into nylon 11 thin film during heat treatment

K. Akamatsu, S. Deki

J. Colloid Interface Sci. 214 353 - 361 1999

Joint Work

（学術雑誌、1999）214, 353-361

Novel preparation method of nano-sized gold particles by using NH2-terminated polyehtylenoxide

S. Deki, K. Sayo, A. Yamada, K. Akamatsu, S. Hayashi

J. Colloid Interface Sci. 214 123 - 125 1999

Joint Work

（学術雑誌、1999）214, 123-125

Synthesis and characterization of gold-palladium bimetallic particles dispersed in polymer thin film matrix

S. Deki, K. Akamatsu, Y. Hatakenaka, M. Mizuhata, A. Kajinami

Nanostruct. Mater. 11 59 - 65 1999

Joint Work

（学術雑誌、1999）11, 59-65

Preparation, characterization and optical properties of nano-sized bimetallic particles dispersed in polymer thin films

K. Akamatsu, Y. Hatakenaka, M. Mizuhata, A. Kajinami, S. Deki

Trans. Mater. Res. Soc. Jpn. 24 109 - 112 1999

Joint Work

（学術雑誌、1999）24, 109-112

A selection of short peptides that interact with a porphyrin as a small target by immobilized phage display Reviewed

J. Kawakami, T. Kitano and N. Sugimoto

Chem. Commun. 1765 - 1766 1999

Development of New Biotechnology for the 21st Century ((]G0002[))-Structural and thermodynamic studies of PNA/DNA hybrids containing single bulge nucleotides-

Mem, Konan Univ., Sci. Ser. 46 61 1999

Single Work

"Direct Evidence of In Vivo Nitric Oxide Production and Inducible Nitric Oxide Synthase mRNA Expression in the Rat Brain of Living Rat During Experimental Meningitis" Reviewed

FUJII Satoshi

J. Cereb. Blood Flow Metab. 19 1175 - 1178 1999

Single Work

Cycloprodigiosin hydrochloride, a new H(+)/Cl(-) symporter, induces apoptosis in human and rat hepatocellular cancer cell lines in vitro and inhibits the growth of hepatocellular carcinoma xenografts in nude mice Reviewed

Yamamoto C, Takemoto H, Kuno K, Yamamoto D, Tsubura A, Kamata K, Hirata H, Yamamoto A, Kano H, Seki T, Inoue K

Hepatology 1999

Cycloprodigiosin hydrochloride inhibits acidification of plant vacuole Reviewed

Nakayasu T, Kawauchi K, Hirata H, Shimmen T

Plant & Cell Physiol 40 1999

Cycloprodigiosin hydrochloride obtained from Pseudoalteromonas denitrificans is a potent antimalarial agent Reviewed

Kim HS, Hayashi M, Shibata Y, Wataya Y, Mitamura T, Horii T, Kawauchi K, Hirata H, Tsuboi S, Moriyama Y

Biol. Pharm. Bull 22 1999

Cycloprodigiosin uncouples H+-pyrophosphatase of plant vacuolar membranes in the presence of chloride ionMaeshima Reviewed

M, Nakayasu, T, Kawauchi, K, Hirata, H, Shimmen, T

Plant & Cell Physiol 1999

Single-Stranded DNA Recognition of a 24 mer Peptide Derived from RecA Protein. Reviewed

S. Nakano and N. Sugimoto

Bull. Chem. Soc. Jpn. 71 2205 - 2210 1998.11

Single Work

Effect of Cation Concentration on Duplex Stability of Nucleic Acids with Unnatural Base Pairs.

中野　修一

Nucleic Acids Symp. Ser. 39 121 - 122 1998.11

Single Work

Synthesis and Applications of Molecularly Imprinted Artificial Receptors

Jun Matsui

1998.3

Single Work

Isolation and characterization of cDNA clones for epidermis-specific and musclespecific benes in Ciona savignyi embryos. Reviewed

NISHIKATA Takahito

Zoological Science 15 ( 2 ) 239 - 246 1998

Single Work

DOI： 10.2108/zsj.15.239

CiNii Article

An alternatively spliced gene encoding a Y-box protein showing maternal expression and tissue-specific zygotic expression in the ascidian embryos.(共著) Reviewed

NISHIKATA Takahito

Development, Growth and Differentiation 40 ( 6 ) 631 - 640 1998

Single Work

DOI： 10.1046/j.1440-169x.1998.00399.x

CiNii Article

Centrosome-attracting body : A novel structure closely related to unequal cleavages in the ascidian embryo. Reviewed

NISHIKATA Takahito

Development, Growth & Differentiation 40 ( 1 ) 85 - 95 1998

Single Work

DOI： 10.1046/j.1440-169X.1998.t01-5-00010.x

CiNii Article

Dispersion of gold nanoparticles into a nylon 11 thin film during heat treatment : in situ optical transmission study

K. Akamatsu, S. Deki

J. Mater. Chem. 8 637 - 640 1998

Joint Work

Preparation and characterization of copper(I) oxide nanoparticles dispersed in polymer matrix

S. Deki, K. Akamatsu, T. Yano, M. Mizuhata, and A. Kajinami

J. Mater. Chem. 8 1865 - 1868 1998

Joint Work

Stability of DNA duplexes containing mirror-image DNA

J. Kawakami, K. Tsujita and N. Sugimoto

Nucleic Acids Symp. Ser. 39 55 - 56 1998

ニューバイオテクノロジー:ファージディスプレイ、インビトロセレクション及びコンビナトリアルケミストリー

川上純司

化学 53 74 1998

Single Work

Minimization of genomic human hepatitis delta virus ribozyme Reviewed

H. Fauzi, A. Chiba, F. Nishikawa, M. Roy, J. Kawakami and S. Nishikawa

Anal. Chim. Acta 365 309 - 317 1998

In vitro selection of aptamers that recognize a monosaccharide

J. Kawakami, Y. Kawase and N. Sugimoto

Anal. Chim. Acta 365 95 - 100 1998

生命関連分子の微視的相互作用(3)-RNA鎖を切断するデオキシリボザイムと金属イオンの関係-

川上純司

甲南大学紀要,理学編 45 125 1998

Single Work

生命関連分子の微視的相互作用(4)-ミスマッチを含むRNA/DNAハイブリッドの安定性に及ぼすミスマッチの隣接塩基対の影響-

川上純司

甲南大学紀要,理学編 45 135 1998

Single Work

"Electron Paramagnetic Resonance Detection of Nitric Oxide in the Brain During Sepsis and Meningitis"

FUJII Satoshi

In"The Proceedings of the 2nd International Conference on Bioradicals" 146 - 148 1998

Single Work

A 24 mer Peptide Derived from RecA Protein can Discriminate a Single-Stranded DNA from a Double-Stranded DNA. Reviewed

N. Sugimoto and S. Nakano

Chem. Commun. 2125 - 2126 1997.11

Single Work

DOI： 10.1039/A705575G

A 24 mer Peptide with Activity of a Nucleic Acid Discrimination and Its Recombination.

中野　修一

First International Peptide Symp. ( P-108 ) 1997.11

Single Work

Experimental Quantitation of Cation Effect on Nucleic Acid Stability.

中野　修一

Nucleic Acids Symp. Ser. 37 147 - 148 1997.11

Single Work

Sandwiching Interaction of Peptides with a Porphyrin. Reviewed

N. Sugimoto and S. Nakano

Chem. Lett. 939 - 949 1997.11

Single Work

Cloning of the ascidian Y-box protein gene with a convenient and reliable amplification protocol of 3' ends of mRNAs.

NISHIKATA Takahito

Memoirs of Konan University, Science Series 44 ( 2 ) 45 - 51 1997

Single Work

Nanoscale metal particles dispersed in polymer matrix

K. Akamatsu, S. Deki

Nanostruct. Mater. 8 1121 - 1129 1997

Joint Work

Characterization and optical properties of gold nanoparticles dispersed in nylon 11 thin films

K. Akamatsu, S. Deki

J. Mater. Chem. 7 1773 - 1777 1997

Joint Work

Evolution of a phosphorothioate RNA library during in vitro selection

J. Kawakami and N. Sugimoto

Nucleic Acids Symp. Ser. 37 201 - 202 1997

生命関連分子の微視的相互作用(II)-ペプチドの分子内α-ヘリックスと分子間β-シートの構造遷移-

川上純司

甲南大学紀要,理学編 44 17 1997

Single Work

Analysis of the cleavage reaction of a trans-acting human hepatitis delta virus ribozyme Reviewed

H. Fauzi, J. Kawakami, F. Nishikawa and S. Nishikawa

Nucleic Acids Research 25 3124 - 3130 1997

A possible immunosuppressant, cycloprodigiosin hydrochloride, obtained from Pseudoalteromonas denitrificans Reviewed

Kawauchi K, Shibutani K, Yagisawa H, Kamata H, Nakatsuji S, Anzai H, Yokoyama Y, Ikegami Y, Moriyama Y, Hirata H

Biochem. Biophys. Res. Commun, 237 1997

Authorship：Lead author

Development of New SPR Chip with an Oligopeptide Recognizing a Single-Stranded DNA.

中野　修一

Nucleic Acids Symp. Ser. 35 303 - 304 1996.11

Single Work

Improved Thermodynamic Parameters and Helix Initiation Factor to Predict Stability of DNA Duplexes. Reviewed

N. Sugimoto, S. Nakano, M. Yoneyama, and K. Honda

Nucleic Acids Res. 24 4501 - 4505 1996.11

Single Work

DOI： 10.1093/nar/24.22.4501

Interactions of 5-(1-pyrenyl)-10,15,20,-tris(4-methyl-pyridinium)porphine with Double-Helix and Triple-Helix Nucleic Acids. Reviewed

N. Sugimoto, S. Nakano, M. Katoh, M. Sasaki, and S. Kugimiya

Nucleosides & Nucleotides 15 743 - 747 1996.8

Single Work

DOI： 10.1080/07328319608002420

Two distinct cell types identified in the ascidian notochord. Reviewed

NISHIKATA Takahito

Zoological Science 13 725 - 730 1996

Single Work

Molecalar characterization of myoplasmin-Cl : a cytoskeletal component localized in the myoplasm of the ascidian egg Reviewed

NISHIKATA Takahito, WADA Michiko

Development, Genes and Evolution 206 72 - 79 1996

Joint Work

DOI： 10.1007/s004270050032

Preparation and characterization of Au-dispersed TiO2 thin films by a liquid-phase deposition method

S. Deki, Y. Aoi, H. Yanagimoto, H. Ishii, K. Akamatsu, M. Mizuhata, A. Kajinami

J. Mater. Chem. 6 1879 - 1882 1996

Joint Work

Selection in vitro of trans-acting genomic human hepatitis delta virus (HDV) ribozymes Reviewed

F. Nishikawa, J. Kawakami, A. Chiba, K. Yuda, M. Shirai, P.K.R. Kumar and S. Nishikawa

Eur. J. Biochem. 327 712 - 718 1996

生命関連分子の微視的相互作用(I)-バルジを含むDNA二重らせんの熱力学的安定性に及ぼすイノシン置換の影響-

川上純司

甲南大学紀要,理学編 43 37 - 47 1996

Single Work

Constructing an efficient trans-acting genomic HDV ribozyme Reviewed

J. Kawakami, K. Yuda, Y.-A. Suh, P.K.R. Kumar, F. Nishikawa, H. Maeda, K. Taira, E. Ohtsuka and S. Nishikawa

FEBS Lett. 394 132 - 136 1996

Thermodynamic Parameters to Predict Stability of RNA/DNA Hybrid Duplexes.

中野　修一

J. Biomol. Struct. Dyn. 12 ( a233 ) 1995.11

Single Work

Interaction of Oligonuleotides with a Single Stranded DNA Binding Site of RecA Protein.

中野　修一

Nucleic Acids Symp. Ser. 34 61 - 62 1995.11

Single Work

Thermodynamic stability of RNA/DNA hybrids. Reviewed

N. Sugimoto, S. Nakano, M. Katoh, A. Matsumura, H. Nakamuta, T. Ohmichi, M. Yoneyama, and M. Sasaki

Biochemistry 34 11211 - 11216 1995.9

Single Work

DOI： 10.1021/bi00035a029

ANALYSIS OF FUNCTIONAL STRUCTURE OF HDV RIBOZYME BY MODIFICATION INTERFERENCE AND IN-VITRO SELECTION-STRATEGIES

S NISHIKAWA, YH JEOUNG, P KUMAR, J KAWAKAMI, F NISHIKAWA, A CHIBA, K YUDA, P VILLJANEN, K TAIRA

JOURNAL OF CELLULAR BIOCHEMISTRY 19A 227 - 227 1995.1

Joint Work

Publisher：WILEY-LISS

Designing of HDV ribozyme by in vitro selection Reviewed

S. Nishikawa, F. Nishikawa, J. Kawakami, A. Chiba, K. Yuda, Y.-H. Jeoung, P.K.R. Kumar and K. Taira

Antisense Research and Development 54 104 - 105 1995

Thermodynamic Parameters to Predict the Stability of RNA/DNA Hybrids in Antisense Interaction.

中野　修一

First International Antisence Conference of Japan ( P1-05 ) 1994.11

Single Work

RNA/DNA Hybrid Duplexes with Identical Nearest-Neighbor Base-Pairs Have Identical Stability. Reviewed

N. Sugimoto, M. Katoh, S. Nakano, T. Ohmichi, and M. Sasaki

FEBS Lett. 354 74 - 78 1994.10

Single Work

DOI： 10.1016/0014-5793(94)01098-6

ヒトδ型肝炎ウイルス（HDV）リボザイムの構造活性相関に関する研究

川上純司

1994.3

Single Work

Specification in the primary lineage of ascidian muscle cells analyzed with a specific monoclonal antibody

NISHIKATA Takahito

Memoirs of Konan University Science Series 41 ( 2 ) 89 - 97 1994

Single Work

Monoclonal antibodies with affinity to self-complementary left-handed DNA containing cyclonucleosides with high anti conformation Reviewed

J. Kawakami, S. Uesugi, M. Ikehara, T. Itoh, K. Miura and E. Ohtsuka

Nucleosides & Nucleotides 13 421 - 427 1994

The accumulation of vanadium and manifestation of an antigen recognized by a monoclonal antibody specific to vanadocytes during embryo genesis in vanadium-rich ascidian, ┣DBAsidia(/)-┫DB ┣DBSydneieusis(/)-┫DB ┣DBsamea(/)-┫DB. Reviewed

NISHIKATA Takahito

Journal of Experimental Zoology 265,29-34 1993

Single Work

Systematic substitution of individual bases in two important single-stranded regions of the HDV ribozyme for evaluation of the role of specific bases Reviewed

Y.-A. Suh, P.K.R. Kumar, J. Kawakami, F. Nishikawa, K. Taira, and S. Nishikawa

FEBS Lett. 326 158 - 162 1993

Chemical probing studies of the hepatitis delta virus (HDV) genomic ribozyme

S. Nishikawa, P.K.R. Kumar, Y.-H. Jeong, J. Kawakami, F. Nishikawa, Y.-A. Suh, E. Ohtsuka and K. Taira

Nucleic Acids Symp. Ser. 29 119 - 120 1993

Identification of important bases in a single-stranded region (SSrC) of the hepatitis delta ribozyme Reviewed

J. Kawakami, P.K.R. Kumar, Y.-A. Suh, F. Nishikawa, K. Kawakami, K. Taira, E. Ohtsuka and S. Nishikawa

Eur. J. Biochem. 217 29 - 36 1993

Initial stages of tunic morphogenesis in the ascidian ┣DBHalocythia(/)-┫DB : a fine structural study. Reviewed

NISHIKATA Takahito

Tissue and Cell 24 1992

Single Work

Random mutations to evaluate the role of bases at two important single-stranded regions of genomic HDV ribozyme Reviewed

P.K.R. Kumar, Y.-A. Suh, H. Miyashiro, F. Nishikawa, J. Kawakami, K. Taira and S. Nishikawa

Nucleic Acids Research 20 3919 - 3924 1992

Ribozymes as molecular scissors for suppressing AIDS virus Reviewed

J. Ohkawa, T. Shimayama, N. Yuyama, S. Sawata, Y. Takagi, A. Wada, K. Yasuda, J. Kawakami, P.K.R. Kumar, Y.-A. Suh, F. Nishikawa, M. Oda, S. Shinshi, M. Uebayashi, T. Uchimaru, K. Furukawa, S. Nishikawa and K. Taira

Now & Future 8 8 - 12 1992

Identification of important bases for the self-cleavage activity at two single-stranded regions of genomic HDV ribozyme

Nucleic Acids Symposium Series 27 41 1992

Single Work

Monoclonal antibody specific to signet ring cells, the vanadocytes of the tunicate, ┣DBAscidia(/)-┫DB ┣DBsydneiensis(/)-┫DB ┣DBsamea(/)-┫DB. Reviewed

NISHIKATA Takahito

Journal of Experimental Zoology 259 1991

Single Work

Characterization of antigenic polypeptides recognized by monoclonal antibodies specific to the myoplasm of eggs of the ascidian ┣DBCiona(/)-┫DB ┣DBinterstinalis(/)-┫DB. Reviewed

NISHIKATA Takahito

Development, Growth and Differentiation 33 1991

Single Work

DOI： 10.1111/j.1440-169X.1991.00459.x

Expression of an antigen specific for trunk-lateral cells in quarter embryos of the ascidian, ┣DBHalocynthia(/)-┫DB ┣DBroretzi(/)-┫DB. Reviewed

NISHIKATA Takahito

Journal of Experimental Zoology 258 1991

Single Work

Specification of notochord cells in the ascidian embryo analysed with a specific monoclonal antibody. Reviewed

NISHIKATA Takahito

Cell Differentiation and Development 30 1990

Single Work

Cellular and molecular mechanisms of muscle cell differentiation in the ascidian embryos. Reviewed

NISHIKATA Takahito

International Review of Cytology 122 1990

Single Work

Egg cytoplasmic components responsible for larval muscle cell differentiation in ascidian embryos.

NISHIKATA Takahito

Developmental Biology UCLA Symposia 125 1990

Single Work

ホヤ卵における筋肉細胞分化決定因子の分子的実体の解析

西方敬人

1989.3

Single Work

A monoclonal antibody specific to embryoie trunk-lateral cells of the ascidian ┣DBHalocynthia(/)-┫DB ┣DBroretzi(/)-┫DB stains coelomic cells of juvenile and adult basophilic blood cells. Reviewed

NISHIKATA Takahito

Development, Growth and Differentiation 31 ( 6 ) 595 - 600 1989

Single Work

DOI： 10.1111/j.1440-169X.1989.00595.x

CiNii Article

Differentiation expression in blastomeres of cleavagearrested embryos of the ascidian ┣DBHalocynthia(/)-┫DB ┣DBroretzi(/)-┫DB. Reviewed

NISHIKATA Takahito

Development, Growth and Differentiation 30 ( 4 ) 1988

Single Work

DOI： 10.1111/j.1440-169X.1988.00371.x

Muscle cell differentiation in ascidian embryos analysed with a tissue-specific monoclonal antibody. Reviewed

NISHIKATA Takahito

Development 99 ( 2 ) 1987

Single Work

Monoclonal antibodies against components of the myoplasm of eggs of the ascidian ┣DBCiona(/)-┫DB ┣DBintestinalis(/)-┫DB partially block the development of muscle-specific acetylcholinesterase. Reviewed

NISHIKATA Takahito

Development 100 ( 2 ) 1987

Single Work

Cell-and tissue-specific monoclonal antibodies in eggs and embryos of the ascidian ┣DBHalocynthia(/)-┫DB ┣DBroretzi(/)-┫DB. Reviewed

NISHIKATA Takahito

Development 99 ( 2 ) 1987

Single Work

Expression of epidermis-specific antigens during embryogenesis of the ascidian, ┣DBHalocynthia(/)-┫DB ┣DBroretzi(/)-┫DB. Reviewed

NISHIKATA Takahito

Developmental Biology 121 ( 2 ) 408 - 416 1987

Single Work

DOI： 10.1016/0012-1606(87)90177-1

PubMed

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