Papers -
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Roles of Loop Region in Folding Kinetics and Transcription Inhibition of DNA G-Quadruplexes Reviewed
Minori Nakata, Naoki Kosaka, Keiko Kawauchi, Daisuke Miyoshi
Biochemistry 64 609 - 619 2025.1
Authorship：Corresponding author
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Factors affecting liquid-liquid phase separation of RGG peptides with DNA G-Quadruplex Reviewed
Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, Daisuke Miyoshi
ChemMedChem 20 ( 2 ) e202400460 2025.1
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The role of cytosine methylation in regulating the topology and liquid-liquid phase separation of DNA G-quadruplexes Reviewed
Mitsuki Tsuruta, Sumit Shil, Shinya Taniguchi, Keiko Kawauchi, Daisuke Miyoshi
Chemical Science 16 ( 10 ) 4213 - 4225 2025.1
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Heterogeneity of Molecular Crowding and Liquid-Liquid Phase Separation. International journal
Mitsuki Tsuruta, Sumit Shil, Keiko Kawauchi, Daisuke Miyoshi
Sub-cellular biochemistry 109 327 - 345 2025
The inside of a living cell is highly crowded with extremely diverse biomacromolecules, small metabolites and osmolytes. The molecular conditions in cells change dynamically and rapidly depending on the cell cycle and state, organelle, and compartment. Much remains unknown regarding how biomolecular interactions and reactions can proceed in a spatiotemporally specific manner in such crowded, heterogeneous, and dynamic molecular environments. Selective condensation/droplet formation of biomolecules via liquid-liquid phase separation may be critical for interactions and reactions inside cells. In this chapter, we briefly describe the heterogeneity of molecular environments inside cells and the biological roles of liquid-liquid phase separation that allows biomolecular interactions and reactions in such heterogenous molecular environments. Finally, we discuss the mutual relationship between molecular crowding and liquid-liquid phase separation.
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Nanocomposite thin layers of in situ-embedded bismuth nanoparticles in a polymer matrix: synthesis and size-dependent thermal properties Reviewed
Chem. Lett 2025
Authorship：Corresponding author
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Development of a New Structural Transformation Reaction in Cr-Based MOFs Induced by Fluoride Reviewed
S. Tanaka, R. Miyata, R. Teramoto, J. Sugimoto, T. Tsuruoka, K. Akamatsu, Y. Takashima
Chem. Asian J 2025
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Controlling Film Formation of Ag-Chalocogenate Coordination Polymer via Ag Ion-Doped Polymer Substrates Reviewed
T. Tsuruoka, R. Oishi, Y. Takashima, K. Akamatsu
Inorganics 13 396 - 396 2025
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Sequence-Specific Free Energy Changes in DNA/RNA Induced by a Single LNA-T Modification in Antisense Oligonucleotides Reviewed
Elisa Tomita-Sudo, Tomoka Akita, Nae Sakimoto, Saori Tahara-Takamine, Junji Kawakami
International Journal of Molecular Sciences 25 13240 2024.12
Authorship：Last author, Corresponding author
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Bioinformatic analysis of actin-binding proteins in the nucleolus during heat shock Invited Reviewed
Shinya Taniguchi, Takeru Torii, Toshiyuki Goto, Kohei Takeuchi, Rine Katsumi, Mako Sumida, Sunmin Lee, Wataru Sugimoto, Masaya Gessho, Katsuhiko Itoh, Hiroaki Hirata, Junji Kawakami, Daisuke Miyoshi, Keiko Kawauchi
Genes 15 1580 2024.12
Authorship：Last author, Corresponding author
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L-Histidine Modulates the Catalytic Activity and Conformational Changes of the HD3 Deoxyribozyme Reviewed
Nae Sakimoto, Hirofumi Imanaka, Elisa Tomita-Sudo, Tomoka Akita, Junji Kawakami
Genes 15 1481 2024.11
Authorship：Last author, Corresponding author
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NRAS DNA G-quadruplex-targeting molecules for sequence-selective enzyme inhibition Reviewed
Yoshiki Hashimoto, Hiroki Kubo, Keiko Kawauchi, Daisuke Miyoshi
Chem. Commun. 60 13179 - 13182 2024.11
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NRAS DNA G-quadruplex-targeting molecules for sequence-selective enzyme inhibition Invited Reviewed
Yoshiki Hashimoto, Hiroki Kubo, Keiko Kawauchi and Daisuke Miyoshi
Chem. Commun. 60 13179 - 13182 2024.10
Authorship：Corresponding author
DOI： 10.1039/D4CC03753G
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The role of cytosine methylation in regulating the topology and liquid-liquid phase separation of DNA G-quadruplexes Reviewed
Mitsuki Tsuruta, Sumit Shil, Shinya Taniguchi, Keiko Kawauchi and Daisuke Miyoshi
Chem. Sci. 16 4213 - 4225 2024.10
Authorship：Corresponding author
DOI： 10.1039/D4SC06959E
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Structural transformation of silver(I)-thiolate coordination plymer film at solid-liquid interfaces Reviewed International coauthorship
Myu Fukuoka, Yohei Takashima, Kensuke Akamatsu, Aude Demessence, Takaaki Tsuruoka
CrystEngComm 26 6269 - 6273 2024.10
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Factors Affecting Liquid-Liquid Phase Separation of RGG Peptides with DNA G-Quadruplex Invited Reviewed
Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, Daisuke Miyoshi
ChemMedChem 2024.9
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Bioinformatic Analysis of Actin-Binding Proteins in the Nucleolus During Heat Shock Invited Reviewed
Shinya Taniguchi, Takeru Torii ,Toshiyuki Goto, Kohei Takeuchi, Rine Katsumi, Mako Sumida, Sunmin Lee, Wataru Sugimoto, Masaya Gessho, Katsuhiko Itoh, Hiroaki Hirata, Junji Kawakami, Daisuke Miyoshi and Keiko Kawauchi
Genes 15 ( 12 ) 1580 2024.9
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Detection of Conformational Changes of G4 Aptamer in the Presence of Different Cations using Calorimetry Reviewed
Elisa Tomita-Sudo, Taku Ishigaki, Masako Hirose, Maki Kato, Junji Kawakami
Netsu Sokutei 51 ( 4 ) 148 - 152 2024.9
Authorship：Last author, Corresponding author
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Non-canonical olfactory pathway activation induces cell fusion of cervical cancer cells. Reviewed International journal
Keigo Araki, Takeru Torii, Kohei Takeuchi, Natsuki Kinoshita, Ryoto Urano, Rinka Nakajima, Yaxuan Zhou, Tokuo Kobayashi, Tadayoshi Hanyu, Kiyoshi Ohtani, Kimiharu Ambe, Keiko Kawauchi
Neoplasia 57 101044 - 101044 2024.8
Authorship：Last author
Multinucleation occurs in various types of advanced cancers and contributes to their malignant characteristics, including anticancer drug resistance. Therefore, inhibiting multinucleation can improve cancer prognosis; however, the molecular mechanisms underlying multinucleation remain elusive. Here, we introduced a genetic mutation in cervical cancer cells to induce cell fusion-mediated multinucleation. The olfactory receptor OR1N2 was heterozygously mutated in these fused cells; the same OR1N2 mutation was detected in multinucleated cells from clinical cervical cancer specimens. The mutation-induced structural change in the OR1N2 protein activated protein kinase A (PKA), which, in turn, mediated the non-canonical olfactory pathway. PKA phosphorylated and activated furin protease, resulting in the cleavage of the fusogenic protein syncytin-1. Because this cleaved form of syncytin-1, processed by furin, participates in cell fusion, furin inhibitors could suppress multinucleation and reduce surviving cell numbers after anticancer drug treatment. The improved anticancer drug efficacy indicates a promising therapeutic approach for advanced cervical cancers.
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Quantitative Effects of the Loop Region on Topology, Thermodynamics, and Cation Binding of DNA G-quadruplexes Reviewed
ACS Omega 9 35028 - 35036 2024.7
Authorship：Last author, Corresponding author
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Development of a CaCO3 Precipitation Method Using a Peptide and Microwaves Generated by a Magnetron Reviewed
Fumihiro Kayamori, Hiroyuki Togashi, Natsumi Endo, Makoto Ozaki, Kan Hirao, Yonejiro Arimoto, Ryuji Osawa, Takaaki Tsuruoka, Takahito Imai, Kin-ya Tomizaki, Tomohiro Umetani, Nobuhiro Nakanishi, Kenji Usui
Processes 12 1327 - 1327 2024.6
Publisher：MDPI AG
Microwave applications, such as microwave ovens and mobile phones, are ubiquitous and indispensable in modern society. As the utilization of microwave technology is becoming more widespread, the effects of microwaves on living organisms and physiological processes have received increased attention. This study aimed to investigate the effects of microwaves on calcium carbonate biomineralization as a model biochemical process. A magnetron oscillator was used to generate 2450 MHz microwaves because magnetrons are relatively inexpensive and widespread. We conducted transmission electron microscopy (TEM), atomic force microscopy (AFM), TEM-electron energy-loss spectroscopy (EELS), dynamic light scattering (DLS), and high-performance liquid chromatography (HPLC) measurements to analyze the calcium carbonate precipitates. Our findings showed the formation of string-like precipitates of calcium carbonate upon microwave irradiation from one direction, similar to those obtained using a semiconductor oscillator, as reported previously. This implied that the distribution of the frequency had little effect on the morphology. Furthermore, spherical precipitates were obtained upon microwave irradiation from two directions, indicating that the morphology could be controlled by varying the direction of microwave irradiation. Magnetrons are versatile and also used in large-scale production; thus, this method has potential in medical and industrial applications.
DOI： 10.3390/pr12071327