Papers -
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Wrapping Flexible Metal-Organic Framework with Organic Polymers via Site-Specific Radical Polymerization from Its Crystal Surface Invited Reviewed
Yohei Takashima, Kenji Fukudome, Atsuto Horikoshi, Takaaki Tsuruoka, Kensuke Akamatsu
Polyhedron 155 275 - 280 2018.11
Joint Work
Authorship：Lead author
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Yohei Takashima, Kenji Fukudome, Atsuto Horikoshi, Takaaki Tsuruoka, Kensuke Akamatsu
Polyhedron 155 275 - 280 2018.11
Joint Work
© 2018 Elsevier Ltd In this study, we succeeded in the crystal surface modification of metal–organic framework (MOF) with an organic polymer by site-specific radical polymerization. A MOF was selected with interpenetrated structure containing a naphthalenediimide moiety with electron accepting capability. N, N-dimethylaniline, as an electron donating guest, induced electron transfer to the napthalenediimide moiety, generating radical anions on the pore and crystal surfaces, initiating radical polymerization. The resulting composite was examined in detail with XRD, TG, TEM, ICP, IR, gas adsorption, and fluorescent measurements, demonstrating that an organic polymer was generated only on the crystal surface, preserving the original porous property. In addition, higher structural stability in response to H2O was observed after polymer wrapping.
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Anti-inflammatory effect of 5-aminolevulinic acid on macrophage in vitro. Reviewed
Ishikawa M, Mashiba R, Kawakatsu K, Nakagawa H, Kitajima Y, Ishizuka M, Nishikata T.
ALA-Porphyrin Science 7 ( 1 ) 3 - 11 2018.9
Joint Work
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Gold-Titania Nanocatalyst Generated by Mineralization Using Two Artificial Peptides with DNA Reviewed
Ozaki Makoto, Tomizaki Kin-Ya, Hamada Yoshio, Usui Kenji
JOURNAL OF PEPTIDE SCIENCE 24 S101 2018.9
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Effects of platinum and palladium nanocolloid on macrophage polarization in relevance to repigmentation of vitiligo. Reviewed
Minamiyama Y, Ishikawa M, Shibata T, Tsuji G, Nishikata T, Takemura S, Ichihashi M.
J Cutan Immunol Allergy 1 139 - 146 2018.8
Joint Work
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巻頭言 変化は突然に ―学会参加のススメ―
三好大輔
日本化学会 生体機能関連化学部会 ニュースレター 33 ( 2 ) 2 - 3 2018.8
Single Work
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Phagocytic activation of macrophages with serum MAF depends on engulfment efficiency and not migratory activity. Reviewed
Mashiba R, Ishikawa M, Sumiya YU, Kawakatsu K, Tran NK, Nishikata T.
Anticancer Res. 38 ( 7 ) 4295 - 4298 2018.7
Joint Work
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An anionic phthalocyanine decreases NRAS expression by breaking down its RNA G-quadruplex Reviewed
Keiko Kawauchi, Wataru Sugimoto, Takatoshi Yasui, Kohei Murata, Katsuhiko Itoh, Kazuki Takagi, Takaaki Tsuruoka, Kensuke Akamatsu, Hisae Tateishi-Karimata, Naoki Sugimoto, and Daisuke Miyoshi
Nature Communicationsvolume 9 2271 2018.6
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An anionic phthalocyanine decreases NRAS expression by breaking down its RNA G-quadruplex Reviewed International journal
Kawauchi K, Sugimoto W, Yasui T, Murata K, Itoh K, Takagi K, Tsuruoka T, Akamatsu K, Tateishi-Karimata H, Sugimoto N, Miyoshi D.
Nature Commun. 9 2271 2018.6
Joint Work
Authorship：Lead author, Corresponding author
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Living functional hydrogels generated by bioorthogonal cross-linking reactions of azide-modified cells with alkyne-modified polymers Reviewed
K. Nagahama, Y. Kimura, A. Takemoto
Nature Communications ( 9 ) 2195 2018.6
Joint Work
Authorship：Lead author
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Thermal stability and conformation of DNA and proteins under the confined condition in the matrix of hydrogels
S. Nakano, D. Yamaguchi, and N. Sugimoto
Mol. Biol. Rep. ４5 403 - 411 2018.4
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Kenji Usui, Shin-Ichiro Yokota, Makoto Ozaki, Shungo Sakashita, Takahito Imai, Kin-Ya Tomizaki
Protein and Peptide Letters 25 42 - 47 2018.4
Joint Work
Authorship：Lead author Publisher：Bentham Science Publishers B.V.
Background: A core sequence (the 9 C-terminal residues) of calcification-associated peptide (CAP-1) isolated from the exoskeleton of the red swamp crayfish was previously shown to control calcium carbonate precipitation with chitin. In addition, a modified core sequence in which the phosphorylated serine at the N terminus is replaced with serine exhibits was also previously shown to alter precipitation characteristics with chitin. Objectives: We focused on calcium carbonate precipitation and attempted to elucidate aspects of the mechanism underlying mineralization. We attempted to evaluate in detail the effects of modifying the N-terminus in the core sequence on calcium carbonate mineralization without chitin. Methods: The peptide modifications included phosphorylation, dephosphorylation, and a free or acetylated Nterminus. The peptides were synthesized manually on Wang resin using the DIPCI-DMAP method for the first residue, and Fmoc solid phase peptide synthesis with HBTU-HOBt for the subsequent residues. Prior to calcium carbonate precipitation, calcium carbonate was suspended in MilliQ water. Carbon dioxide gas was bubbled into the stirred suspension, then the remaining solid CaCO3 was removed by filtration. The concentration of calcium ions in the solution was determined by standard titration with ethylenediaminetetraacetate. Calcium carbonate precipitation was conducted in a micro tube for 3 h at 37°C. We used the micro-scale techniques AFM (atomic force microscopy) and TEM (transmission electron microscopy), and the macro-scale techniques chelate titration, HPLC, gel filtration, CD (circular dichroism) and DLS (dynamic light scattering). Results: We determined the morphologies of the calcium carbonate deposits using AFM and TEM. The pS peptide provided the best control of the shape and size of the calcium carbonate round particles. The acetylated peptides (Ac-S and Ac-pS) provided bigger particles with various shapes. S peptide provided a mixture of bigger particles and amorphous particles. We verified these findings using DLS. All the peptide samples produced nanostructures of the expected size in agreement with the AFM and TEM results. We estimated the abilities of these peptides to precipitate calcium carbonate by determining the residual calcium hydrogen carbonate concentration by standard titration with ethylenediaminetetraacetate after calcium carbonate precipitation. The Ac-pS peptide showed the lowest residual calcium hydrogen carbonate concentration whereas the S peptide showed the highest, suggesting that the precipitating activities of these peptides towards calcium carbonate correlated with peptide net charge. Then the gel filtration results showed a large oligomer peak and a small oligomer/monomer peak for all peptide samples in agreement with the AFM, TEM and DLS results. CD measurements showed that all the peptides formed random-coil-like structures. Thus, we used both macro-and micro-observation techniques such as chelate titration, DLS, AFM and TEM to show that the calcium carbonate precipitating activities of four derivatives of the core sequence of CAP-1 may correlate with the peptide net charge. Conclusion: These peptides mainly act as a catalyst rather than as a binder or component of the calcium carbonate deposits (as a template). On the other hand, the morphologies of the calcium carbonate deposits appeared to be dependent on the ability of the peptide to assemble and act as a template. Consequently, elucidating the relationship between peptide sequence and the ability of the peptide to assemble would be indispensable for controlling precipitate morphologies in the near future. This knowledge would provide important clues for elucidating the relationship between peptide sequence and mineralization ability, including deposit morphology and precipitating activity, for use in nanobiochemistry and materials chemistry research.
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Yuki Tominaga, Kenji Usui, Akiyoshi Hirata, Hiro-O Ito, Kiyoshi Nokihara
Bioorganic and Medicinal Chemistry 26 3210 - 3216 2018.4
Joint Work
Authorship：Lead author Publisher：Elsevier Ltd
A fundamental method has been developed focusing on a facile and rapid examination of periodontal disease. Periodontal disease is an oral disease thought to affect 80% of adults, and early detection with treatment is desirable for the improvement of the quality of life. Unfortunately conventional methods are not consistent as the disease is caused by a number of undefined bacteria and detection relies on the skills of the dentist. Thus an objective detection system is required. We have performed an experiment on saliva using a novel biodetection system, designated PepTenChip®. A disease model for saliva was prepared using a specimen from a healthy subject and a mixture of hemoglobin (f-Hb) and lactate dehydrogenase (LDH), which is used as a periodontal disease marker protein with healthy saliva. PepTenChip® is a peptide microarray in which fluorescent labelled structured peptides are immobilized on a novel amorphous carbon substrate. Since the peptides used as capture molecules are fluorescently labelled, labeling of analytes is not necessary. The fluorescence intensity change before and after application of analytes are detected rather than the ON/OFF detection common to conventional microarrays using a set of antigen–antibody. The fluorescence intensity value changes according to the concentration of captured protein allowing the generation of protein fingerprint (PFP) and dendrograms. The present method does not rely on a “one to one” interaction, unlike conventional biodetection, and advantages can be envisaged in the case of an undefined or unknown cause of disease. The statistical analyses, such as multivariate analyses, allow classification of the type of proteins added in saliva as mimetics of disease. PepTenChip® system is useful and convenient for examination of periodontal disease in health care.
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Peptides for Silica Precipitation: Amino Acid Sequences for Directing Mineralization Reviewed
Makoto Ozaki, Shungo Sakashita, Yoshio Hamada, Kenji Usui
Protein & Peptide Letters 25 15 - 24 2018.4
Joint Work
Publisher：Bentham Science Publishers B.V.
Background: Peptides are promising compounds for use in inorganic or organic-inorganic hybrid syntheses (mineralization) and offer several advantages over proteins. Meanwhile, silica-based nanomaterials have been extensively investigated for many years because of their potential application in a diverse range of technologies, including catalysis, sensing, separation, enzyme immobilization, and gene and drug delivery. Considerable progress has been made over the past decade in understanding the molecular mechanisms underpinning biosilicification and the biomimetic synthesis of patterned nanosilica using peptides. Objectives: This mini-review focuses on various peptide sequences, especially short peptide sequences (30 residues or less), for silica mineralization. Methods: We first briefly review early studies on silica mineralization using proteins to provide background information. This is followed by a discussion of promising peptide sequences and attempts to discern the relationship between amino acid sequence, their potential for mineralization, and the properties of the mineral product. Results: The synthetic control of silica mineralization using engineered proteins, such as recombinant silicateins and silaffins, was inspired by silica biomineralization by natural proteins from organisms (sponges, diatoms, and plants). Concurrently, several papers described the utility of well-structured protein assemblies as templates for silica mineralization. These template-directed syntheses of well-structured silica deposits were first conducted using natural proteins or protein assemblies such as collagen fibers and virus hollow protein tubes. Then we reviewed a selection of short peptides (30 residues or less) that had been successfully used for silica mineralization. Almost all peptides developed to date can be sorted by classification like proteins (synthetic control of silica mineralization or utility of templates for silica mineralization): the first class of peptides is used for peptide-directed synthesis, and the second is used for template-directed synthesis after the peptides have assembled and formed nanostructure such as fibers and tubes. The presented peptides were classified and arranged according to the classification. Additionally, we briefly introduced silica mineralization triggered by the combination of short silica-precipitating peptides and template molecules. Conclusion: In this mini-review we focused on various peptide sequences, especially short peptide sequences of 30 residues or less, designed for silica mineralization. The peptides have been used both for peptide-directed silica mineralization and for template-directed silica mineralization. The recent advances in peptide-driven mineralization reviewed here suggest that it will soon be possible to completely control the silica mineralization process using peptides. Mineralization systems using peptides will provide researchers with new tools for controlling various inorganic syntheses and the production of organic-inorganic materials for nanobiochemistry and materials chemistry research.
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Editorial: Organic-Inorganic Hybrid Materials and Their Applications Reviewed
Kin-ya Tomizaki, Yoshio Hamada, Kenji Usui
Protein & Peptide Letters 25 2 - 3 2018.4
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Substrate rigidity-dependent positive feedback regulation between YAP and ROCK2. Reviewed International journal
Sugimoto W, Itoh K, Mitsui Y, Ebata T, Fujita H, Hirata H, Kawauchi K
Cell adhesion & migration 12 ( 2 ) 101 - 108 2018.3
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Nanocomposite injectable gels capable of self-replenishing regenerative extracellular microenvironments for in vivo tissue engineering Reviewed
K. Nagahama, N. Oyama, K. Ono, A. Hotta, K. Kawauchi, T. Nishikata
Biomater Sci. 6 ( 3 ) 550 - 561 2018.2
Joint Work
DOI： 10.1039/c7bm01167a.
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Nanocomposite injectable gels capable of self-replenishing regenerative extracellular microenvironments for in vivo tissue engineering Reviewed International journal
Koji Nagahama, Naho Oyama, Kimika Ono, Atsushi Hotta, Keiko Kawauchi, Takahito Nishikata
Biomater Sci. 6 550 - 561 2018.2
Joint Work
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A high-throughput quantitative assay system for macrophage phagocytic activity. Reviewed
Mami Ishikawa, Riho Mashiba, Kumpei Kawakatsu, Ngoc Kiet Tran, Takahito Nishikata
macrophage 2018.2
Joint Work
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Nanocomposite injectable gels capable of self-replenishing regenerative extracellular microenvironments for in vivo tissue engineering Reviewed
K. Nagahama, N. Oyama, K. Ono, A. Hotta, K. Kawauchi, T. Nishikata
Biomaterials Science ( 6 ) 550 - 561 2018.2
Joint Work
Authorship：Lead author