Faculty of Frontiers of Innovative Research in Science and Technology

Papers -

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Thermodynamic properties of water molecules in the presence of cosolute depend on DNA structure: a study using grid inhomogeneous solvation theory Reviewed

M. Nakano, H. Tateishi-Karimata, S. Tanaka, F. Tama, O. Miyashita, S. Nakano, and N. Sugimoto

Nucleic Acids Res. 43 10114 - 10125 2015.11

Joint Work

DOI： 10.1093/nar/gkv1133

A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis Reviewed

K. Usui, K.-y. Tomizaki, H. Mihara

Methods. Mol. Biol. 1352 199 - 210 2015.11

Joint Work

Authorship：Lead author

p53 regulates cytoskeleton remodeling to suppress tumor progression.

Araki K, Ebata T, Guo AK, Tobiume K, Wolf SJ, Kawauchi K

Cellular and molecular life sciences : CMLS 72 ( 21 ) 4077 - 4094 2015.11

Joint Work

DOI： 10.1007/s00018-015-1989-9

PubMed

Facile DNA Detection Based on Fluorescence Switching of A Hydrophobic AIE Dye-Labeled Peptide Nucleic Acid Probe by Aggregation/Disaggregation Reviewed

K. Kawamura, A. Matsumoto, T. Murashima

International Journal of Medical Nano Research 2 ( 2 ) 2015.9

Joint Work

DOI： 10.23937/2378-3664/1410011

Cryoprotective ability of betaine-type metabolite analogs during freezing denaturation of enzymes International journal

Yuichi Nakagawa, Masahiro Sota, Kazuya Koumoto

BIOTECHNOLOGY LETTERS 37 ( 8 ) 1607 - 1613 2015.8

Publisher：SPRINGER

To evaluate an analog library of betaine-type cellular metabolites, which are naturally found in polar fish for survival in subzero temperatures, for preventing denaturation of enzymes during freezing.Comparison of the cryoprotective ability of reported cryoprotectants, such as dimethylsulfoxide, glycerol, ectoine, hydroxyectoine, and trehalose, with betaine-type analogs using alpha-glucosidase revealed that analogs introducing C-3-C-6 alkyl chains into an ammonium cation retained 20 % higher activity than the control cryoprotectants at the same concentration. In particular, the analog possessing triplicate n-butyl chains showed a profound effect. It allowed retention of enzyme activity to 95 % even after 100 freeze-thaw cycles, while addition of the control cryoprotectants decreased the activity to 10-20 %. The cryoprotective ability of betaine-type analogs can be applied not only to alpha-glucosidase but also other enzymes such as beta-glucosidase, alkaline phosphatase, lactose dehydrogenase, sulfatase, and horseradish peroxidase.Synthetic betaine-type metabolite analogs possess practicable cryoprotective ability for various enzymes, and are considerably superior to previously reported cryoprotectants.

DOI： 10.1007/s10529-015-1841-1

PubMed

Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size Reviewed International journal

Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui

BIOCONJUGATE CHEMISTRY 26 ( 8 ) 1775 - 1781 2015.8

Publisher：AMER CHEMICAL SOC

The design and synthesis of materials capable of activating the immune system in a safe manner is of great interest in immunology and related fields. Lactobacilli activate the innate immune system of a host when acting as probiotics. Here, we constructed lactobacilli-mimicking materials in which polysaccharide peptidoglycan complexes (PS PGs) derived from lactobacilli were covalently conjugated to the surfaces of polymeric microparticles with a wide variety of sizes, ranging from 200 nm to 3 mu m. The artificial lactobacilli successfully stimulated macrophages without cytotoxicity. Importantly, we found that the size of artificial lactobacilli strongly influenced their immunostimulating activities, and that artificial lactobacilli of 1 mu m exhibited 10-fold higher activity than natural lactobacilli. One major advantage of the artificial lactobacilli is facile control of size, which cannot be changed in natural lactobacilli. These findings provide new insights into the design of materials for immunology as well as the molecular biology of lactobacillus.

DOI： 10.1021/acs.bioconjchem.5b00319

PubMed

Establishment of a macrophage-activating factor (MAF) assay system using human-monocytic cell line, THP-1. Reviewed

Inoue T., Ishikawa M., Sumiya Y., Kohda H, Inui T., Kuchiike D., Kubo K., Uto Y and Nishikata T.

Anticancer Research 35 4441 - 4445 2015.8

Joint Work

Oral Colostrum Macrophage-activating Factor for Serious Infection and Chronic Fatigue Syndrome: Three Case Reports. Reviewed

Inui T., Kubo K., Kuchiike D., Uto Y., Nishikata T., Sakamoto N., Mette M.

Anticancer Research 35 ( 8 ) 4545 - 4549 2015.8

Joint Work

Macrophage Activation Mechanisms in Human Monocytic Cell Line-derived Macrophages. Reviewed

Sumiya Y, Ishikawa M, Inoue T, Inui T, Kuchiike D, Kubo K, Uto Y, Nishikata T

Anticancer research 35 ( 8 ) 4447 - 4451 2015.8

Joint Work

PubMed

Establishment of a Macrophage-activating Factor Assay System Using the Human Monocytic Cell Line THP-1 Reviewed

Takahiro Inoue, Mami Ishikawa, Yu Sumiya, Haruka Kohda, Toshio Inui, Daisuke Kuchiike, Kentaro Kubo, Yoshihiro Uto, Takahito Nishikata

ANTICANCER RESEARCH 35 ( 8 ) 4441 - 4445 2015.8

Joint Work

Publisher：INT INST ANTICANCER RESEARCH

Background: As the mechanism of macrophage activation is not well-understood, standardization of an assay system for measuring phagocytic activities of macrophages will be useful for research on macrophages. Previously, we established a novel standardized macrophage-activating factor (MAF) assay system using U937. Materials and Methods: Using the human monocytic cell line THP-1, another standardized MAF assay system was established. Characteristic gene expression of U937- and THP-1-derived macrophages was compared by gene expression microarray analysis. Results: Both U937- and THP-1-derived macrophages showed obvious phagocytic activities with unique characteristics and, therefore, could not be assigned to a single sub-type. Conclusion: Activation of macrophages is an intricate cellular process. Comparison of our two novel assay systems provides new insights into macrophage activation mechanisms.

Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size Reviewed

Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, and Jun Matsui

Bioconjugate Chemistry 26 1775 - 1781 2015.8

Joint Work

Authorship：Lead author

Effects of background anionic compounds on the activity of the hammerhead ribozyme in Mg2+-unsaturated solutions Reviewed

S. Nakano, Y. Kitagawa, D. Miyoshi, and N. Sugimoto

J. Biol. Inorg. Chem 20 ( 6 ) 1049 - 1058 2015.7

Joint Work

Authorship：Lead author

DOI： 10.1007/s00775-015-1286-y

Effects of cosolvents on the folding and catalytic activities of the hammerhead ribozyme Reviewed

S. Nakano, Y. Kitagawa, H. Yamashita, D. Miyoshi, and N. Sugimoto

ChemBioChem 16 ( 12 ) 1803 - 1810 2015.7