Papers -
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Towards Imaging Electron Density inside Metal-Organic Framework Structures Reviewed
Yohei Takashima, De-Liang Long, Leroy Cronin
Chem. Commun 50 2271 - 2274 2014
Joint Work
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Enhanced Phosphorescence Emission by Incorporating Aromatic Halides into an Entangled Coordination Framework Based on Naphthalenediimide Reviewed
Virginia Mart?nez-Mart?nez, Rebeca Sola Llano, Shuhei Furukawa, Yohei Takashima, I?igo L?pez Arbeloa, Susumu Kitagawa
ChemPhysChem 15 2517 - 2521 2014
Joint Work
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In situ synthesis of metal/polymer nanocomposite thin films on glass substrates by using highly cross-linked polymer matrices with tailorable ion exchange capabilities Reviewed
I. Toda, T. Tsuruoka, J. Matsui, T. Murashima, H. Nawafune, K. Akamatsu
RSC Advances 4 4723 - 4726 2013.12
Joint Work
DOI： 10.1039/C3RA46166A
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Control of Site-Specific Silica Precipitation Using PNA Peptides and DNAs Reviewed
K. Usui, H. Nishiyama, K. Nagai, T. Tsuruoka, S. Fujii, K.-y. Tomizaki
Peptides 2013 162 - 163 2013.12
Joint Work
Authorship：Lead author
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Designed Peptide Libraries for Cell Analyzing Microarrays Reviewed
H. Mihara, K. Usui, H. Tsutsumi, K. Nokihara
Peptides 2013 22 - 23 2013.12
Joint Work
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Peptide-DNA Hybrid G-Quadruplex Structures for Regulation of Protein Expression Depending on Protease Activity Reviewed
A. Okada, M. Taniguchi, K. Usui, N. Sugimoto
Peptides 2013 180 - 181 2013.12
Joint Work
Authorship：Lead author
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Cytoplasmic translocation of the retinoblastoma protein disrupts sarcomeric organization.
Araki K, Kawauchi K, Hirata H, Yamamoto M, Taya Y
eLife 2 e01228 2013.12
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Positional Effect of 2',4'-BNA/LNA Introduction into DNA/RNA Duplexes on Thermodynamic Parameters
S. Tahara, E. Tomita, H. Nagai, S. Obika, and J. Kawakami
Proc. 40th Intl. Symp. Nucleic Acid Chemistry 190 - 191 2013.11
Joint Work
Authorship：Lead author
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A highly sensitive telomerase activity assay that eliminates false-negative results caused by PCR inhibitors Reviewed
H. Yaku, T. Murashima, D. Miyoshi, N. Sugimoto
Molecules 18 ( 10 ) 11751 - 11767 2013.9
Joint Work
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Multiple and cooperative binding of fluorescence light-up probe thioflavin t with human telomere DNA G-quadruplex Reviewed
V. Gabelica, R. Maeda, T. Fujimoto, H. Yaku, T. Murashima, N. Sugimoto, D. Miyoshi
Biochemistry 52 ( 33 ) 5620 - 5628 2013.7
Joint Work
DOI： 10.1021/bi4006072
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Conformational changes of the phenyl and naphthyl isocyanate-DNA adducts during DNA replication and by minor groove binding molecules Reviewed
S. Nakano, Y. Uotani, Y. Sato, H. Oka, M. Fujii, and N. Sugimoto
Nucleic Acids Res. 41 8581 - 8590 2013.7
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In situ template synthesis of one-dimensional gold nanoparticle arrays in organic nanowires Reviewed
T. Matsushita, Y. Fukumoto, T. Kawakami, T. Tsuruoka, T. Murashima, T. Yanagishita, H. Masuda, H. Nawafune, K. Akamatsu
RSC Advances 3 16243 - 16246 2013.7
Joint Work
DOI： 10.1039/C3RA42727G
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Quantitative analyses of nucleic Acid stability under the molecular crowding condition induced by cosolutes Invited Reviewed
Tateishi-Karimata H, Nakano S, Sugimoto N.
Curr Protoc Nucleic Acid Chem. Chapter 7 Unit7.19 2013.6
Joint Work
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Systematic screening of the cellular uptake of designed alpha-helix peptides Reviewed
Kenji Usui, Takuya Kikuchi, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
BIOORGANIC & MEDICINAL CHEMISTRY 21 ( 9 ) 2560 - 2567 2013.5
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
The cellular penetration (CP) activity of functional molecules has attracted significant attention as one of the most promising new approaches for drug delivery. In particular, cell-penetrating peptides (CPPs) have been studied extensively in cellular engineering. Because there have been few large-scale systematic studies to identify peptide sequences with optimal CP activity or that are suitable for further applications in cell engineering, such as cell-specific penetration and cell-selective culture, we screened and compared the cellular uptake (CU) activity of 54 systematically designed a-helical peptides in HeLa cells. Furthermore, the CU activity of 24 designed peptides was examined in four cell lines using a cell fingerprinting technique and statistical approaches. The CU activities in various cells depended on amino acid residues of peptide sequences as well as charge, a-helical content and hydrophobicity of the peptides. Notably, the mutation of a single residue significantly altered the CU ability of a peptide, highlighting the variability of cell uptake mechanisms. Moreover, these results demonstrated the feasibility of cell-selective culture by conducting cell-selective permeation and death in cultures containing two cell types. These studies may lead to further peptide library design and screening for new classes of CPPs with useful functions. (C) 2013 Elsevier Ltd. All rights reserved.
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A peptide release system using a photo-cleavable linker in a cell array format for cell-toxicity analysis Invited Reviewed
Takashi Kakiyama, Kenji Usui, Kin-ya Tomizaki, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
POLYMER JOURNAL 45 ( 5 ) 525 - 539 2013.5
Joint Work
Authorship：Lead author Publisher：NATURE PUBLISHING GROUP
We constructed a novel peptide-array format system for cellular toxicity analysis. In this system, a peptide was immobilized on a conventional 96-well plate bottom via a photo-cleavable linker. Once UV light irradiated the desired wells, the peptide was released from the bottom. As a result, the cytotoxic behavior of the peptide could be monitored. Immobilization and light-irradiation conditions were optimized. The immobilized peptide showed no cytotoxicity; therefore, the cells could be cultured on the peptide-immobilizing plate from the beginning of the experiment. Cell-toxicity assays with this system for three cell types were performed. All cell types showed similar to 25% lowering of viability with the photo-released 5-(and-6)-carboxytetramethylrhodamine (TMR)-GKLAKLAKKLAKLAKKLAKLAKGC (TMR-KLA-C) peptide compared with the non-coated plate. This relative toxicity nearly corresponded to that of similar to 10 mu M TMR-KLA-C in solution, and we found that the released peptide concentration per well was similar to 10 mu M at 60 min irradiation. Throughout this study, we successfully immobilized peptides via the photo-cleavable linker, released them by UV irradiation spatiotemporally and conducted the cell-toxicity assay. This study implies that the peptide photo-releasing array system will allow the realization of high-throughput cell arrays for cellomics analyses and cell-based phenotypic drug screenings.
DOI： 10.1038/pj.2013.20
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Biodegradable polymers exhibiting temperature-responsive sol-gel transition as injectable biomedical materials Reviewed
Koji Nagahama, Akihiro Takahashi, Yuichi Ohya
Reactive and Functional Polymers ( 73 ) 979 - 985 2013.5
Joint Work
Authorship：Lead author
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Study on effects of molecular crowding on G-quadruplex-ligand binding and ligand-mediated telomerase inhibition Reviewed
H. Yaku, T. Murashima, H. Tateishi-Karimata, S. –I. Nakano, D. Miyoshi, N. Sugimoto
Methods 64 ( 1 ) 19 - 27 2013.4
Joint Work
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A PNA Peptide for the Control of Site-Specific Silica Precipitation on DNA Reviewed
Kenji Usui, Kazuma Nagai, Hiroto Nishiyama, Takaaki Tsuruoka, Satoshi Fujii
Peptide Science 2012 375 - 376 2013.3
Joint Work
Authorship：Lead author
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Construction of Artificial Peptides for Control of DNA G-quadruplex Structures Depending on Protease Activity toward Regulation of Protein Expression Reviewed
Arisa Okada, Kenji Usui, Mai Taniguchi, Keita Kobayashi, Naoki Sugimoto
Peptide Science 2012 285 - 286 2013.3
Joint Work
Authorship：Lead author
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Enhanced stereocomplex formation of enantiomeric polylactides grafted on a polyrotaxane platform Reviewed
Koji Nagahama, Rie Aoki, Toshifumi Saito, Tatsuro Ouchi, Yuichi Ohya and Nobuhiko Yui
Polymer Chemistry ( 4 ) 1769 - 1773 2013.3
Joint Work
Authorship：Lead author