Papers -
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DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity Reviewed
Kenji Usu, Arisa Okada, Shungo Sakashita, Masayuki Shimooka, Takaaki Tsuruoka, Shu-ichi Nakano, Daisuke Miyoshi, Tsukasa Mashima, Masato Katahira and Yoshio Hamada
Molecules 22 1991 2017.11
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Influence of intracellular environment on allosteric ribozyme activity
Misaki Kameno, Mika Sawada, Nae Sakimoto and Junji Kawakami
Proc. 44th Intl. Symp. Nucleic Acid Chemistry 178 - 179 2017.11
Joint Work
Authorship：Lead author
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Quantification of stabilization effect of co-solutes on RNA tertiary interaction
Natsumi Sasaki, Daisuke Miyoshi and Junji Kawakami
Proc. 44th Intl. Symp. Nucleic Acid Chemistry 174 - 175 2017.11
Joint Work
Authorship：Lead author
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Non-Covalent Loading of Anti-Cancer Doxorubicin by Modularizable Peptide Self-Assemblies for a Nanoscale Drug Carrier Reviewed
Kin-ya Tomizaki, Kohei Kishioka, Shunsuke Kataoka, Makoto Miyatani, Takuya Ikeda, Mami Komada, Takahito Imai, Kenji Usui
MOLECULES 22 ( 11 ) 1916 2017.11
Joint Work
Authorship：Lead author Publisher：MDPI AG
We prepared nanoscale, modularizable, self-assembled peptide nanoarchitectures with diameters less of than 20 nm by combining beta-sheet-forming peptides tethering a cell-penetrating peptide or a nuclear localization signal sequence. We also found that doxorubicin (Dox), an anti-cancer drug, was non-covalently accommodated by the assemblies at a ratio of one Dox molecule per ten peptides. The Dox-loaded peptide assemblies facilitated cellular uptake and subsequent nuclear localization in HeLa cells, and induced cell death even at low Dox concentrations. This peptide nanocarrier motif is a promising platform for a biocompatible drug delivery system by altering the targeting head groups of the carrier peptides.
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細胞環境化学模倣実験系におけるDNAの構造とその熱力学的安定性
三好大輔
日本核酸化学会誌 1 13 - 19 2017.10
Single Work
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Newly characterized interaction stabilizes DNA structure: oligoethylene glycols stabilize G-quadruplexes CH–π interactions Reviewed
H. Tateishi-Karimata, T. Ohyama, T. Muraoka, P. Podbevsek, A. M. Wawro, S. Tanaka, S. Nakano, K. Kinbara, J. Plavec, N. Sugimoto
Nucleic Acids Res. 45 7021 - 7030 2017.7
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Fluorescent and luminescent fusion proteins for analyses of amyloid beta peptide aggregation Reviewed
Kenji Usui, Masayasu Mie, Takashi Andou, Hisakazu Mihara, Eiry Kobatake
JOURNAL OF PEPTIDE SCIENCE 23 ( 7-8 ) 659 - 665 2017.7
Joint Work
Authorship：Lead author Publisher：WILEY
The amyloid beta (A) peptide is regarded as a causative agent of Alzheimer's disease. In this study, fluorescent and luminescent fusion proteins were constructed to analyze A aggregation. A system was developed to monitor changes in luminescence that provides information about A aggregation. In the presence of monomeric A, the fusion protein exhibits higher luminescence intensity, and the luminescence intensity is diminished after aggregation of the fusion protein and A. In contrast, the fluorescence is sustained in the presence of A. In the absence of A, the fusion protein self-aggregates, and its luminescence and fluorescence are quenched, thus decreasing the background fluorescence and enhancing the detection of A inside and outside the cells. The ratio of the luminescence intensity to the fluorescence intensity would allow the aggregation degrees of A to be distinguished. This study would be a promising method for analyzing the aggregation state of a particular amyloid protein/peptide (monomer, oligomer, or fibril), as well as the distribution of the amyloid protein/peptide within and at the cell surface, by using a single fusion protein. Copyright (c) 2017 European Peptide Society and John Wiley & Sons, Ltd.
DOI： 10.1002/psc.3003
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Quantitative relationship between chemical properties and bioactivities of anti-microRNA oligonucleotides targeted to tumor-associated microRNA-21 Reviewed
Koji Nagahama, Kenta Iseda, Daichi Kawano, Junji Kawakami
BIOCHIMIE 137 124 - 131 2017.6
Joint Work
Authorship：Last author, Corresponding author Publisher：ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Synthetic anti-microRNA oligonucleotides (AMOs) are promising drug candidates to inactivate disease related microRNAs because of their sequence-specific binding to their targets and the variety of chemical modifications available. Over the last decade, the qualitative relationships between the chemical properties of AMOs and bioactivity (inactivation of their target miRNAs) have been studied to enhance their bioactivity. On the other hand, in real-world drug development, drugs must be designed case-by case, taking many factors into account. Thus, in order to design AMOs that target specific miRNA, understanding the quantitative relationship between the chemical properties of AMOs and inactivation of their target miRNA is necessary. Here, we aimed to find the specific quantitative relationship of AMOs targeted to tumor-associated miR-21 through direct comparison of their inactivation efficacies with systematically varied chemical properties, including sequence-specific binding affinity, nuclease resistance, and RNase H activation. As a result, we newly found the quantitative relationships; (1) sequence specific binding affinity of AMOs against miR-21 is the main determining factor for inactivation efficacy, (2) nuclease resistance of AMOs impacts their miR-21 inactivation efficacy acting cooperatively with the binding affinity, although nuclease resistance alone does not affect the miRNA inactivation efficacy, and (3) RNase H activation is unnecessary. This study also demonstrates the utility of the obtained relationship for the design of AMO-based drugs targeted to miR-21, through cell-based analyses. Thus, the obtained quantitative relationship would make it possible to predict the miR-21 inactivation efficacy of AMOs which are newly designed. (C) 2017 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
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Quantitative relationship between chemical properties and bioactivities of anti-microRNA oligonucleotides targeted to tumor-associated microRNA-21 Reviewed
Koji Nagahama, Kenta Iseda, Daichi Kawano, Junji Kawakami
Biochimie 137 124 - 131 2017.6
Joint Work
Authorship：Lead author
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NF-kappaB prevents oncogenic Ras-induced b-actin cleavage in p53-deficient cells Reviewed
Wataru Sugimoto, Katsuhiko Itoh, Toshiya Kotari, Alvin K. Guo,Takahiro Ebata, Hiroaki Hirata, Keiko Kawauchi
International Journal of Cancer & Cellular Biology Research 2 ( 1 ) 15 - 18 2017.5
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Highly Sensitive Telomerase Assay Insusceptible to Telomerase and Polymerase Chain Reaction Inhibitors for Cervical Cancer Screening Using Scraped Cells. Reviewed
Hidenobu Yaku , Yoshio Yoshida, Hidehiko Okazawa, Yasushi Kiyono, Yuko Fujita, Daisuke Miyoshi
Anal. Chem. 89 ( 13 ) 6948 - 6953 2017.5
Joint Work
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PEG-based nanocomposite hydrogel: Thermoresponsive sol-gel transition controlled by PLGA-PEG-PLGA molecular weight and solute concentration Reviewed
Makoto Miyazaki, Tomoki Maeda, Kenji Hirashima, Naruki Kurokawa, Koji Nagahama, Atsushi Hotta
Polymer 115 246 - 254 2017.4
Joint Work
Authorship：Lead author
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Quantitative relationship between chemical properties and bioactivities of anti-microRNA oligonucleotides targeted to tumor-associated microRNA-21 Reviewed
Koji Nagahama, Kenta Iseda, Daichi Kawano, Junji Kawakami
Biochimie 137 124 - 131 2017.3
Joint Work
Authorship：Lead author
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Water Solubilization of Fullerene Derivatives by beta-(1,3-1,6)-D-Glucan and Their Photodynamic Activities toward Macrophages Reviewed International journal
Atsushi Ikeda, Tatsuya Iizuka, Naotake Maekubo, Kazuyuki Nobusawa, Kouta Sugikawa, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Motofusa Akiyama
CHEMISTRY-AN ASIAN JOURNAL 12 ( 10 ) 1069 - 1074 2017.3
Joint Work
Publisher：WILEY-V C H VERLAG GMBH
Anionic and neutral fullerene derivatives were dissolved in water by using beta-(1,3-1,6)-D-glucan (beta-1,3-glucan) as a solubilizing agent. In the water-solubilized complexes, the concentrations of fullerene derivatives were approximate to 0.30 mm and the average particle sizes were approximate to 90 nm. The beta-1,3-glucan-complexed fullerene derivative with a carboxylic acid was found to have higher photodynamic activity toward macrophages under visible-light irradiation (lambda > 610 nm) than other beta-1,3-glucan-complexed fullerene derivatives. This result suggests that carboxylic acid moieties in the complex enhance the binding affinity with beta-1,3-glucan receptors on the surface of macrophages when the beta-1,3-glucan is recognized. In contrast, all beta-1,3-glucan-complexed fullerene derivatives showed no photodynamic activity toward HeLa cells under the same conditions.
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Development of Peptide Microarray to Detect the Protein in Saliva for Periodontal Disease Test Reviewed
Yuki Tominaga, Kenji Usui, Akiyoshi Hirata, Atsushi Kitagawa, Hiro-O Ito and Kiyoshi Nokihara
Peptide Science 2016 131 - 132 2017.3
Joint Work
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Formation of beta-(1,3-1,6)-D-glucan-complexed [70]fullerene and its photodynamic activity towards macrophages Reviewed International journal
Atsushi Ikeda, Motofusa Akiyama, Kouta Sugikawa, Kazuya Koumoto, Yuta Kashijima, Jiawei Li, Toshio Suzuki, Takeshi Nagasaki
ORGANIC & BIOMOLECULAR CHEMISTRY 15 ( 9 ) 1990 - 1997 2017.2
Joint Work
Publisher：ROYAL SOC CHEMISTRY
[70] Fullerene was dissolved in water by complexation with beta-1,3-glucan using a mechanochemical highspeed vibration milling apparatus. The photodynamic activity of beta-1,3-glucan-complexed C-70 was highly dependent on the expression level of dectin-1 on the cell surfaces of macrophages. The photodynamic activity increased as a result of a synergistic effect between beta-1,3-glucan-complexed 1 '-acetoxychavicol acetate and the C-70 complex.
DOI： 10.1039/C6OB02747D
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Cell and Molecular Mechanics in Health and Disease Invited Reviewed
BioMed Research International Article ID 2860241 2017.2
Joint Work
DOI： 10.1155/2017/2860241
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Nicotine-Selective Polymeric Adsorbent Obtained by Molecular Imprinting with Excess Use of Itaconic Acid Reviewed
Haruka Nogami, Yuma Nakahori, Takashi Murashima, Jun Matsui
Chromatography 38 15 - 21 2017.1
Joint Work
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Substrate stiffness influences doxorubicin- induced p53 activation via ROCK2 expression Reviewed
Ebata T, Mitsui Y, Sugimoto W, Maeda M, Araki K, Machiyama H, Harada I, Sawada Y, Fujita H, Hirata H, Kawauchi K
BioMed Research International 2017.1
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Unexpected Position-Dependent Effects of Ribose G‑Quartets in G‑Quadruplexes Reviewed
J. Zhou, S. Amrane, F. Rosu, G.F. Salgado, Y. Bian, H. Tateishi-Karimata, E. Largy, D. N. Korkut, A. Bourdoncle, D. Miyoshi, J. Zhang, H. Ju, W. Wang, N. Sugimoto, V. Gabelica, and J. Mergny
J. Am. Chem. Soc. 139 7768 - 7779 2017
Joint Work