Papers -
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The effect of the structure of cosolutes on the DNA duplex formation
中野 修一
Nucleic Acids Symp. Ser. 50 205 - 206 2006.11
Joint Work
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Recognition of the base pair-mimic nucleosides by DNA polymerases
中野 修一
Nucleic Acids Symp. Ser. 50 201 - 202 2006.11
Joint Work
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Protein-fingerprint data mining of a designed alpha-helical peptide array Reviewed
Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara
MOLECULAR BIOSYSTEMS 2 ( 9 ) 417 - 420 2006.9
Publisher：ROYAL SOC CHEMISTRY
The data generated from protein fingerprints with an a-helical peptide array were analyzed using several statistical methods such as hierarchical clustering analysis and principal component analysis to discriminate target proteins. The data generated from protein fingerprints with an a-helical peptide array were analyzed using several statistical methods such as hierarchical clustering analysis and principal component analysis to discriminate target proteins.
DOI： 10.1039/b608875a
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A novel stable RNA pentaloop that interacts specifically with a motif peptide of lambda-N protein（共著） Reviewed
J. Kawakami, H. Tokitoh, Y. Tanabe, and N. Sugimoto
Nucleoside, Nucleotides and Nucleic Acids 25 397 - 416 2006.4
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The negative regulation of p53 by hedgehog signaling Reviewed
Abe Yoshinori, Oda-Sato Eri, Tobiume Kei, Kawauchi Keiko, Okamoto Koji, Taya Yoichi, Tanaka Nobuyuki
CANCER RESEARCH 66 ( 8 ) 2006.4
Joint Work
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A novel peptide microarray for protein detection and analysis utilizing a dry peptide array system Reviewed
K Usui, K Tomizaki, T Ohyama, K Nokihara, H Mihara
MOLECULAR BIOSYSTEMS 2 ( 2 ) 113 - 121 2006.2
Publisher：ROYAL SOC CHEMISTRY
A novel dry peptide microarray system has been constructed that affords a practical solution for protein detection and analysis. This system is an array preparation and assay procedure tinder dry conditions that uses designed peptides as non-immobilized capture agents for the detection of proteins. The system has several advantages that include its portability and ease-Of-Use, as well as the fact that vaporization of sample solutions need not be considered. In this study, various proteins have been characterized with an a-helical peptide mini-library. When proteins were added to the peptide library array, the fluorescent peptides showed different fluorescent intensities depending on their sequences. The patterns of these responses could be regarded as protein fingerprints' (PFPs), which are sufficient to establish the identities of the target proteins. Furthermore, statistical analysis of the resulting PFPs was performed using cluster analysis. The PFPs of the proteins were clustered successfully depending on their families and binding properties. Additionally, the target protein was characterized using a nanolitre system and could be detected down to 1.2 fmol. These Studies imply that the dry peptide array system is a promising too] for detecting and analyzing target proteins. The dry peptide array will play a role in development of high-throughput protein-detecting nano/micro arrays for proteomics and ligand screening studies.
DOI： 10.1039/b514263r
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A PNA-DNA hybridization chip approach for the detection of beta-secretase activity Reviewed
S Sano, KY Tomizaki, K Usui, H Mihara
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 16 ( 3 ) 503 - 506 2006.2
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
Developed was the addressable chip technology based on the PNA-DNA complementary hybridization equipped with short seven-mer PNA-encoded peptides that can be a versatile scaffold to monitor on-chip immunoassays. We also developed and validated a methodology to perform beta-secretase enzyme assay with a highly sensitive fashion, resulting that a peptide substrate tethering dual fluorescent probes allowed us to detect beta-secretase activity 10 times more sensitively than assays in solution. (c) 2005 Elsevier Ltd. All rights reserved.
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A DNA duplex with extremely enhanced thermal stability based on controlled immobilization on gold nanoparticles Reviewed
K. Akamatsu, M. Kimura, Y. Shibata, S. Nakano, D. Miyoshi, H. Nawafune, and N. Sugimoto
Nano Lett. 3 491 - 495 2006.1
Joint Work
DOI： 10.1021/nl0524748
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Use of the polymorphic nature of high-order DNA structures for the design of dynamic and functional nanodevices Invited Reviewed
D. Miyoshi, N. Sugimoto
Recent Devel. Nucleic Acids Res. 2 49 - 70 2006.1
Joint Work
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Use of the polymorphic nature of high-order DNA structures for the design of dynamic and functional nanodevices
D. Miyoshi, N. Sugimoto
Recent Devel. Nucleic Acids Res. 2 49 - 70 2006.1
Joint Work
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Photodimerization of Anthryl Moieties in a Poly(methacrylic acid) Derivative as Reversible Cross-linking Step in Molecular Imprinting
J. Matsui, Y. Ochi, K. Tamaki
Chem. Lett. 80 - 81 2006
Joint Work
Authorship：Lead author
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Molecularly-Imprinted Polymeric Logic Gates Selective for Predetermined Chemical Input Species
松井淳
Chem. Comm. 3217 - 3219 2006
Single Work
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Dynamic redistribution of vasa homolog and exclusion of somatic cell determinants during germ cell specification in Ciona intestinalis.
西方敬人
Development ( 133 ) 2683 - 2693 2006
Single Work
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UV-irradiated cells as a screening system for the antioxidants.
西方敬人
Proceedings of the 13th Biennial Congress of the International Society for Free Radical Research -SFRR 253 - 256 2006
Single Work
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A DNA duplex with Extremely Enhanced Thermal Stability Based on Controlled Immobilization on Gold Nanoparticles
K. Akamatsu, M. Kimura, Y. Shibata, S. Nakano, D. Miyoshi, H. Nawafune, N. Sugimoto
Nano Lett. 6 491 - 495 2006
Joint Work
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金属イオンレドックス系無電解めっき浴の安定性
赤松謙祐、増田昭仁、尾山祐斗、縄舟秀美
表面技術 57 799 - 801 2006
Joint Work
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Fabrication of fluorescent multilayers consisting of CdTe nanocrystals
T. Tsuruoka, R. Takahashi, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn. 31 425 - 428 2006
Joint Work
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Designed peptide microarrays for protein detection and characterization Reviewed
Kenji Usui, Kin-ya Tomizaki, Kiyoshi Nokihara, Hisakazu Mihara
UNDERSTANDING BIOLOGY USING PEPTIDES 731 - + 2006
Publisher：SPRINGER
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High throughput preparation of peptide arrays containing two fluorescent byes focusing on practical protein detection systems Reviewed
Kiyoshi Nokihara, Takafumi Ohyama, Koichi Yonemura, Yasuo Oka, Kenji Usui, Hisakazu Mihara
UNDERSTANDING BIOLOGY USING PEPTIDES 738 - + 2006
Joint Work
Publisher：SPRINGER
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Site-Selective Integration of Monolayer-Protected Inorganic Nanoparticles onto Surface Monolayer Templates by a Solvent-Induced Lift-Off Process Reviewed
K. Akamatsu, S. Samitsu, T. Tsuruoka, J. Hasegawa, H. Nawafune
Small 2 1130 - 1133 2006
Joint Work