Papers -
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Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface Reviewed
T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu
J. Colloid Interface Sci 451 212 - 215 2015
Joint Work
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Metal Nanocrystal/Metal Organic Framework Core/Shgell Nanostructure From Selective Self-Assembly Induced By Localization of Metal Ion Precursors on Nanocrystal Surface
T. Ohhashi, T. Tsuruoka, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu
J. Colloid Interface Sci 451 212 - 215 2015
Joint Work
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Comparison of Kinetic and Thermodynamic Analyses for Duplex Formation
E. Tomita, M. Sumimoto, S. Itoh, M. Takashima, and J. Kawakami
Proc. 42nd Intl. Symp. Nucleic Acid Chemistry 142 - 143 2015
Joint Work
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Curcumisome nanovesicles generated by self-assembly of curcumin amphiphiles toward cancer theranostics
Koji Nagahama, Takayuki Kumano, Naho Oyama, Junji Kawakami
BIOMATERIALS SCIENCE 3 ( 12 ) 1566 - 1578 2015
Joint Work
Publisher：ROYAL SOC CHEMISTRY
Curcumin (CCM) is an important molecule for achieving cancer theranostics because CCM is a naturally-occurring biocompatible material that exhibits both anticancer activity and strong fluorescence property that can be used for bio-imaging. However, CCM has never been utilized in clinical trials due to its extremely low water solubility, its rapid hydrolysis in aqueous conditions at neutral pH, and its low cellular uptake into cancer cells. Taking advantage of the strong hydrophobicity, p-conjugated frameworks, and ketone and enol groups that generate hydrogen bonds in CCM, we herein fabricated novel CCM-based biodegradable nanovesicles, which we termed as "curcumisome", through the self-assembly of amphiphilic CCM-poly(ethylene glycol) conjugates in aqueous media to develop multifunctional nanobiomaterials for use in cancer theranostics. A high CCM loading content in the curcumisomes was achieved, and the curcumisomes showed high water dispersibility with improved hydrolysis resistance. Importantly, the curcumisomes were effectively internalized into cancer cells and exhibited strong fluorescence for a long period, which is favorable for cancer cell imaging, although only a small amount of the curcumisomes penetrated into normal cells and showed very weak fluorescence. Moreover, curcumisomes effectively induced apoptosis of cancer cells. Thus, curcumisomes may act as multifunctional nanobiomaterials for the development of CCM-based cancer theranostics.
DOI： 10.1039/c5bm00212e
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Control of guanine-rich DNA secondary structures depending on the protease activity using a designed PNA peptide Reviewed
Kenji Usui, Arisa Okada, Keita Kobayashi, Naoki Sugimoto
ORGANIC & BIOMOLECULAR CHEMISTRY 13 ( 7 ) 2022 - 2025 2015
Publisher：ROYAL SOC CHEMISTRY
We constructed a regulation system for DNA secondary structure formation of G-rich sequences using a designed PNA peptide exhibiting an on-to-off switching functionality, depending on the protease activity. This study introduces the new concept of a simple and powerful system for regulating quadruplex-related important biological events.
DOI： 10.1039/c4ob02535k
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Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface Reviewed
T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu
J. Colloid Interface Sci. 451 212 - 215 2015
Joint Work
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Site-Specific Mineralization of Silica and Calcium on DNAs Using a Designed Peptide Reviewed
K. Usui, H. Nishiyama, A. Yamada, M. Ozaki, T. Tsuruoka, K.-y. Tomizaki
Peptide Science 325 - 326 2015
Joint Work
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Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface Reviewed
T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu
J. Colloid Interface Sci 451 212 - 215 2015
Joint Work
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Direct Fabrication of Metal Patterns on Resin Substrate by Combining Imprint and Electrochemical Lithography Reviewed
Yusuke Kimura, Ryosuke Fujiwara, Takaaki Tsuruoka, Yohei Takashima, Hidemi Nawafune, Hiroshi Yanagimoto, Kensuke Akamatsu
J. Surf. Finish. Soc. Jpn 66 18 - 19 2015
Joint Work
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Dependence of Crystal Size on the Catalytic Performance of a Porous Coordination Polymer Reviewed
Tomokazu Kiyonaga, Masakazu Higuchi, Takashi Kajiwara, Yohei Takashima, Jingui Duan, Kazuro Nagashima, Susumu Kitagawa
Chem. Commun 51 2728 - 2730 2015
Joint Work
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Preparation of hydrogels for the study of the effects of spatial confinement on DNA Reviewed
S. Nakano, M. Yoshida, D. Yamaguchi, and N. Sugimoto
Trans. Mat. Res. Soc. Jpn. 39 435 - 438 2014.12
Joint Work
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A novel ribozyme core to perform self-cleavage reaction
S. Tanaka, N. Sakimoto, M. Kameno, N. Sasaki, and J. Kawakami
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 340 - 341 2014.11
Joint Work
Authorship：Lead author
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Thermodynamic Features Provided by an ENA Introduction to Nucleic Acid Drugs
E. Tomita, M. Sumimoto, and J. Kawakami
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 188 - 189 2014.11
Joint Work
Authorship：Lead author
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Effects of oligonucleotide length and locked nucleic acid content on splice-switching oligonucleotide activity
T. Shimo, K. Tachibana, K. Saito, T. Yoshida, E. Tomita, R. Waki, T. Yamamoto, T. Doi, T. Inoue, J. Kawakami, and S. Obika
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 332 - 333 2014.11
Joint Work
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Understanding p53: new insights into tumor suppression.
Kawauchi K, Wolf SJ
Expert review of anticancer therapy 14 ( 10 ) 1101 - 1103 2014.10
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Mechanistic study on the facilitation of enzymatic hydrolysis by alpha-glucosidase in the presence of betaine-type metabolite analogs Reviewed
Yuichi Nakagawa, Shiro Sehata, Satoshi Fujii, Hiroaki Yamamoto, Akihiko Tsuda, Kazuya Koumoto
TETRAHEDRON 70 ( 35 ) 5895 - 5903 2014.9
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
Recently we reported that betaine-type metabolite analogs structure-dependently facilitate enzymatic hydrolysis reaction for alpha-glucosidases, beta-glucosidases, and alkaliphosphatases. To understand the facilitation mechanism for enzymes, in this study we expanded the analog library and measured the properties of analog solutions. The structural investigation on alpha-glucosidase-mediated hydrolysis reaction indicated that suitable structures to facilitate the enzyme reaction efficiently should have the ammonium cation in the betaine structure possess triplicate aliphatic chains from Cl to C7 without any polar functional groups. Analyses of the solution properties revealed that such analogs possess a large hydration layer with low water density. Such a specific hydration environment is generated by the characteristic structure of the betaine-type metabolite analogs. The characteristic hydration indirectly regulates enzyme activity and stability. These findings not only increase our understanding enzyme activation by betaine-type metabolite analogs, but also will contribute to the molecular design of enzyme regulators. (C) 2014 Elsevier Ltd. All rights reserved.
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Refinement and verification of test conditions for colony-forming unit assay in dog bone marrow cells with preservable colony specimens. Reviewed
Takamatsu K., Wakata A., Hirota R., Tabata H., and Nishikata T.
Comparative Clinical Pathology 23 ( 5 ) 1133 - 1139 2014.9
Joint Work
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Cellular differentiation assessments by measuring the degree of cellular internalization and membrane adsorption using designed peptides Reviewed
Kenji Usui, Takuya Kikuchi, Kunio Kikuchi, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 24 ( 17 ) 4129 - 4131 2014.9
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
We demonstrate examples of cellular differentiation assessments, including cellular neurite outgrowth and fat cell maturation, by measuring the degree of membrane adsorption or cellular internalization using designed peptides. Because changes in the cellular membrane and cytosol during differentiation were shown to influence membrane adsorption and cellular internalization, we could successfully evaluate the extent of differentiation simply like stain indicators. (C) 2014 Elsevier Ltd. All rights reserved.
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Roles of the amino group of purine bases in the thermodynamic stability of DNA base pairing Reviewed
S. Nakano and N. Sugimoto
Molecules 19 ( 8 ) 11613 - 11627 2014.8
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Case Report: A Breast Cancer Patient Treated with GcMAF, Sonodynamic Therapy and Hormone Therapy. Reviewed
Inui T, Makita K, Miura H, Matsuda A. Kuchiike D, Kubo K, Mette M, Uto Y, Nishikata T, Hori H, and Sakamoto N.
Anticancer Research 34 ( 8 ) 4589 - 4593 2014.8
Joint Work