Papers -
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A peptide release system using a photo-cleavable linker in a cell array format for cell-toxicity analysis Invited Reviewed
Takashi Kakiyama, Kenji Usui, Kin-ya Tomizaki, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
POLYMER JOURNAL 45 ( 5 ) 525 - 539 2013.5
Joint Work
Authorship：Lead author Publisher：NATURE PUBLISHING GROUP
We constructed a novel peptide-array format system for cellular toxicity analysis. In this system, a peptide was immobilized on a conventional 96-well plate bottom via a photo-cleavable linker. Once UV light irradiated the desired wells, the peptide was released from the bottom. As a result, the cytotoxic behavior of the peptide could be monitored. Immobilization and light-irradiation conditions were optimized. The immobilized peptide showed no cytotoxicity; therefore, the cells could be cultured on the peptide-immobilizing plate from the beginning of the experiment. Cell-toxicity assays with this system for three cell types were performed. All cell types showed similar to 25% lowering of viability with the photo-released 5-(and-6)-carboxytetramethylrhodamine (TMR)-GKLAKLAKKLAKLAKKLAKLAKGC (TMR-KLA-C) peptide compared with the non-coated plate. This relative toxicity nearly corresponded to that of similar to 10 mu M TMR-KLA-C in solution, and we found that the released peptide concentration per well was similar to 10 mu M at 60 min irradiation. Throughout this study, we successfully immobilized peptides via the photo-cleavable linker, released them by UV irradiation spatiotemporally and conducted the cell-toxicity assay. This study implies that the peptide photo-releasing array system will allow the realization of high-throughput cell arrays for cellomics analyses and cell-based phenotypic drug screenings.
DOI： 10.1038/pj.2013.20
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Biodegradable polymers exhibiting temperature-responsive sol-gel transition as injectable biomedical materials Reviewed
Koji Nagahama, Akihiro Takahashi, Yuichi Ohya
Reactive and Functional Polymers ( 73 ) 979 - 985 2013.5
Joint Work
Authorship：Lead author
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Study on effects of molecular crowding on G-quadruplex-ligand binding and ligand-mediated telomerase inhibition Reviewed
H. Yaku, T. Murashima, H. Tateishi-Karimata, S. –I. Nakano, D. Miyoshi, N. Sugimoto
Methods 64 ( 1 ) 19 - 27 2013.4
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A PNA Peptide for the Control of Site-Specific Silica Precipitation on DNA Reviewed
Kenji Usui, Kazuma Nagai, Hiroto Nishiyama, Takaaki Tsuruoka, Satoshi Fujii
Peptide Science 2012 375 - 376 2013.3
Joint Work
Authorship：Lead author
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Construction of Artificial Peptides for Control of DNA G-quadruplex Structures Depending on Protease Activity toward Regulation of Protein Expression Reviewed
Arisa Okada, Kenji Usui, Mai Taniguchi, Keita Kobayashi, Naoki Sugimoto
Peptide Science 2012 285 - 286 2013.3
Joint Work
Authorship：Lead author
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Enhanced stereocomplex formation of enantiomeric polylactides grafted on a polyrotaxane platform Reviewed
Koji Nagahama, Rie Aoki, Toshifumi Saito, Tatsuro Ouchi, Yuichi Ohya and Nobuhiko Yui
Polymer Chemistry ( 4 ) 1769 - 1773 2013.3
Joint Work
Authorship：Lead author
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Design of Biodegradable Injectable Polymers Exhibiting Temperature-Responsive Sol-Gel Transition Reviewed
Y. Ohya, H. Suzuki, K. Nagahama, A. Takahashi, T. Ouchi, A. Kuzuya
Advances in Science and Technology ( 86 ) 9 - 16 2013.3
Joint Work
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Thermodynamics - Hydration Relationships within Loops that Affect G-Quadruplexes under Molecular Crowding Conditions Reviewed
Takeshi Fujimoto, Shu-ichi Nakano, Naoki Sugimoto, Daisuke Miyoshi
J. Phys. Chem. B 117 963 - 972 2013.1
Joint Work
Authorship：Lead author
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Thermodynamics-hydration relationships within loops that affect G-quadruplexes under molecular crowding conditions Reviewed
T. Fujimoto, S. Nakano, N. Sugimoto, and D. Miyoshi
J. Phys. Chem. B 117 ( 4 ) 963 - 972 2013.1
Joint Work
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Biochemical purification of mitochondrial HSP60 and ATPsynthase from ascidian eggs: Implications for antibody specificity Reviewed
Janet Chenevert, Gerard Pruliere, Hirokazu Ishii, Christian Sardet, Takahito Nishikata
PLoS One 8 ( 1 ) e52996 2013.1
Joint Work
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Multiple and cooperative binding of fluorescence light-up probe Thioflavin T with human telomere DNA G-quadruplex Reviewed
Valerie Gabelica, Ryuichi Maeda, Takeshi Fujimoto, Hidenobu Yaku, Takashi Murashima, Naoki Sugimoto, and Daisuke Miyoshi
Biochemistry 52 5620 - 5628 2013
Joint Work
Authorship：Lead author
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A Highly Sensitive Telomerase Activity Assay that Eliminates False-Negative Results Caused by PCR Inhibitors Reviewed
Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, and Naoki Sugimoto
Molecules 18 ( 10 ) 11751 - 11767 2013
Joint Work
Authorship：Lead author
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Sequence and Solvent Effects on Telomeric DNA Bimolecular G-Quadruplex Folding Kinetics Reviewed
Adrien Marchand, Ruben Ferreira, Hisae Tateishi-Karimata, Daisuke Miyoshi, Naoki Sugimoto, Valerie Gabelica
J. Phys. Chem. B 117 ( 41 ) 12391 - 12401 2013
Joint Work
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Recognition of Perfluorooctanoic Acid (PFOA) by Fluorous Molecularly Imprinted Polymer
34 ( Supplement1 ) 21 - 22 2013
Joint Work
Authorship：Lead author
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Application of a Reflectometric Interference Spectroscopic Device (RIfS) with a Polymer-gel Coated SiN Chip to an HPLC Detector
Yuma Nakahori, Yusuke Sugahara, Jun Matsui
Chromatography 34 ( Supplement1 ) 51 - 52 2013
Joint Work
Authorship：Lead author
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Primary culture of breast cancer: A model system for epithelial-mesenchymal transition and cancer stem cells Reviewed
Nishikata T., Ishikawa M., Matsuyama T., Takamatsu K., Fukuhara T., and Konishi Y.
Anticancer Research 33 2867 - 2874 2013
Joint Work
Authorship：Lead author
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Purification of mitochondrial proteins HSP60 and ATP synthase from ascidian eggs: implications for antibody specificity. Reviewed
Chenevert J, Pruliere G, Ishii H, Sardet C, Nishikata T
PloS one 8 ( 1 ) e52996 2013
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Phosphorus and Boron Codoped Colloidal Silicon Nanocrystals with Inorganic Atomic Ligandas Reviewed
H.Sugimoto, M.Fujii, K.Imakita, S.Hayashi, K.Akamatsu
J.Phys Chem.C. 117 6807 - 6813 2013
Joint Work
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Synthesis of Polystyrene-based Nanocomposite Thin Films with Domain Structure Consisting of Au Nanoparticles Reviewed
T. Tsuruoka, S. Kumazaki, I. Osaka, H. Nawafune, K. Akamatsu
Journal of Physics: Conference Series 417 012020 - 012020 2013
Joint Work
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In Situ Template Synthesis of One-dimensional Gold Nanoparticle Arrays in Organic Nanowires Reviewed
T. Matsushita, Y. Fukumoto, T. Kawakami, T. Tsuruoka, T. Murashima, T. Yanagishita, H. Masuda, H. Nawafune, K. Akamatsu
RSC Advances 3 16243 - 16246 2013
Joint Work