論文
-
In situ template synthesis of one-dimensional gold nanoparticle arrays in organic nanowires 査読あり
T. Matsushita, Y. Fukumoto, T. Kawakami, T. Tsuruoka, T. Murashima, T. Yanagishita, H. Masuda, H. Nawafune, K. Akamatsu
RSC Advances 3 16243 - 16246 2013年7月
共著
DOI： 10.1039/C3RA42727G
-
Quantitative analyses of nucleic Acid stability under the molecular crowding condition induced by cosolutes 招待あり 査読あり
Tateishi-Karimata H, Nakano S, Sugimoto N.
Curr Protoc Nucleic Acid Chem. Chapter 7 Unit7.19 2013年6月
共著
-
Systematic screening of the cellular uptake of designed alpha-helix peptides 査読あり
Kenji Usui, Takuya Kikuchi, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
BIOORGANIC & MEDICINAL CHEMISTRY 21 ( 9 ) 2560 - 2567 2013年5月
出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD
The cellular penetration (CP) activity of functional molecules has attracted significant attention as one of the most promising new approaches for drug delivery. In particular, cell-penetrating peptides (CPPs) have been studied extensively in cellular engineering. Because there have been few large-scale systematic studies to identify peptide sequences with optimal CP activity or that are suitable for further applications in cell engineering, such as cell-specific penetration and cell-selective culture, we screened and compared the cellular uptake (CU) activity of 54 systematically designed a-helical peptides in HeLa cells. Furthermore, the CU activity of 24 designed peptides was examined in four cell lines using a cell fingerprinting technique and statistical approaches. The CU activities in various cells depended on amino acid residues of peptide sequences as well as charge, a-helical content and hydrophobicity of the peptides. Notably, the mutation of a single residue significantly altered the CU ability of a peptide, highlighting the variability of cell uptake mechanisms. Moreover, these results demonstrated the feasibility of cell-selective culture by conducting cell-selective permeation and death in cultures containing two cell types. These studies may lead to further peptide library design and screening for new classes of CPPs with useful functions. (C) 2013 Elsevier Ltd. All rights reserved.
-
T. Kakiyama, K. Usui, K.-y. Tomizaki, M. Mie, E. Kobatake, H. Mihara
Polym. J. 45 ( 5 ) 525 - 539 2013年5月
-
Biodegradable polymers exhibiting temperature-responsive sol-gel transition as injectable biomedical materials 査読あり
Koji Nagahama, Akihiro Takahashi, Yuichi Ohya
Reactive and Functional Polymers ( 73 ) 979 - 985 2013年5月
共著
担当区分：筆頭著者
-
Study on effects of molecular crowding on G-quadruplex-ligand binding and ligand-mediated telomerase inhibition 査読あり
H. Yaku, T. Murashima, H. Tateishi-Karimata, S. –I. Nakano, D. Miyoshi, N. Sugimoto
Methods 64 ( 1 ) 19 - 27 2013年4月
共著
-
A PNA Peptide for the Control of Site-Specific Silica Precipitation on DNA 査読あり
Kenji Usui, Kazuma Nagai, Hiroto Nishiyama, Takaaki Tsuruoka, Satoshi Fujii
Peptide Science 2012 375 - 376 2013年3月
共著
担当区分：筆頭著者
-
Construction of Artificial Peptides for Control of DNA G-quadruplex Structures Depending on Protease Activity toward Regulation of Protein Expression 査読あり
Arisa Okada, Kenji Usui, Mai Taniguchi, Keita Kobayashi, Naoki Sugimoto
Peptide Science 2012 285 - 286 2013年3月
共著
担当区分：筆頭著者
-
Enhanced stereocomplex formation of enantiomeric polylactides grafted on a polyrotaxane platform 査読あり
Koji Nagahama, Rie Aoki, Toshifumi Saito, Tatsuro Ouchi, Yuichi Ohya and Nobuhiko Yui
Polymer Chemistry ( 4 ) 1769 - 1773 2013年3月
共著
担当区分：筆頭著者
-
Design of Biodegradable Injectable Polymers Exhibiting Temperature-Responsive Sol-Gel Transition 査読あり
Y. Ohya, H. Suzuki, K. Nagahama, A. Takahashi, T. Ouchi, A. Kuzuya
Advances in Science and Technology ( 86 ) 9 - 16 2013年3月
共著
-
Thermodynamics - Hydration Relationships within Loops that Affect G-Quadruplexes under Molecular Crowding Conditions 査読あり
Takeshi Fujimoto, Shu-ichi Nakano, Naoki Sugimoto, Daisuke Miyoshi
J. Phys. Chem. B 117 963 - 972 2013年1月
共著
担当区分：筆頭著者
-
Thermodynamics-hydration relationships within loops that affect G-quadruplexes under molecular crowding conditions 査読あり
T. Fujimoto, S. Nakano, N. Sugimoto, and D. Miyoshi
J. Phys. Chem. B 117 ( 4 ) 963 - 972 2013年1月
共著
-
Biochemical purification of mitochondrial HSP60 and ATPsynthase from ascidian eggs: Implications for antibody specificity 査読あり
Janet Chenevert, Gerard Pruliere, Hirokazu Ishii, Christian Sardet, Takahito Nishikata
PLoS One 8 ( 1 ) e52996 2013年1月
共著
-
Multiple and cooperative binding of fluorescence light-up probe Thioflavin T with human telomere DNA G-quadruplex 査読あり
Valerie Gabelica, Ryuichi Maeda, Takeshi Fujimoto, Hidenobu Yaku, Takashi Murashima, Naoki Sugimoto, and Daisuke Miyoshi
Biochemistry 52 5620 - 5628 2013年
共著
担当区分：筆頭著者
-
A Highly Sensitive Telomerase Activity Assay that Eliminates False-Negative Results Caused by PCR Inhibitors 査読あり
Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, and Naoki Sugimoto
Molecules 18 ( 10 ) 11751 - 11767 2013年
共著
担当区分：筆頭著者
-
Sequence and Solvent Effects on Telomeric DNA Bimolecular G-Quadruplex Folding Kinetics 査読あり
Adrien Marchand, Ruben Ferreira, Hisae Tateishi-Karimata, Daisuke Miyoshi, Naoki Sugimoto, Valerie Gabelica
J. Phys. Chem. B 117 ( 41 ) 12391 - 12401 2013年
共著
-
Recognition of Perfluorooctanoic Acid (PFOA) by Fluorous Molecularly Imprinted Polymer
Jun Matsui, Megumi Takayose
Chromatography 34 ( Supplement1 ) 21 - 22 2013年
共著
担当区分：筆頭著者
-
Application of a Reflectometric Interference Spectroscopic Device (RIfS) with a Polymer-gel Coated SiN Chip to an HPLC Detector
Yuma Nakahori, Yusuke Sugahara, Jun Matsui
Chromatography 34 ( Supplement1 ) 51 - 52 2013年
共著
担当区分：筆頭著者
-
Primary culture of breast cancer: A model system for epithelial-mesenchymal transition and cancer stem cells 査読あり
Nishikata T., Ishikawa M., Matsuyama T., Takamatsu K., Fukuhara T., and Konishi Y.
Anticancer Research 33 2867 - 2874 2013年
共著
担当区分：筆頭著者
-
Purification of mitochondrial proteins HSP60 and ATP synthase from ascidian eggs: implications for antibody specificity. 査読あり
Chenevert J, Pruliere G, Ishii H, Sardet C, Nishikata T
PloS one 8 ( 1 ) e52996 2013年