論文
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Thermodynamic properties of water molecules in the presence of cosolute depend on DNA structure: a study using grid inhomogeneous solvation theory 査読あり
M. Nakano, H. Tateishi-Karimata, S. Tanaka, F. Tama, O. Miyashita, S. Nakano, and N. Sugimoto
Nucleic Acids Res. 43 10114 - 10125 2015年11月
共著
DOI： 10.1093/nar/gkv1133
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A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis 査読あり
K. Usui, K.-y. Tomizaki, H. Mihara
Methods. Mol. Biol. 1352 199 - 210 2015年11月
共著
担当区分：筆頭著者
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p53 regulates cytoskeleton remodeling to suppress tumor progression.
Araki K, Ebata T, Guo AK, Tobiume K, Wolf SJ, Kawauchi K
Cellular and molecular life sciences : CMLS 72 ( 21 ) 4077 - 4094 2015年11月
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Facile DNA Detection Based on Fluorescence Switching of A Hydrophobic AIE Dye-Labeled Peptide Nucleic Acid Probe by Aggregation/Disaggregation 査読あり
K. Kawamura, A. Matsumoto, T. Murashima
International Journal of Medical Nano Research 2 ( 2 ) 2015年9月
共著
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Cryoprotective ability of betaine-type metabolite analogs during freezing denaturation of enzymes 国際誌
Yuichi Nakagawa, Masahiro Sota, Kazuya Koumoto
BIOTECHNOLOGY LETTERS 37 ( 8 ) 1607 - 1613 2015年8月
出版者・発行元：SPRINGER
To evaluate an analog library of betaine-type cellular metabolites, which are naturally found in polar fish for survival in subzero temperatures, for preventing denaturation of enzymes during freezing.Comparison of the cryoprotective ability of reported cryoprotectants, such as dimethylsulfoxide, glycerol, ectoine, hydroxyectoine, and trehalose, with betaine-type analogs using alpha-glucosidase revealed that analogs introducing C-3-C-6 alkyl chains into an ammonium cation retained 20 % higher activity than the control cryoprotectants at the same concentration. In particular, the analog possessing triplicate n-butyl chains showed a profound effect. It allowed retention of enzyme activity to 95 % even after 100 freeze-thaw cycles, while addition of the control cryoprotectants decreased the activity to 10-20 %. The cryoprotective ability of betaine-type analogs can be applied not only to alpha-glucosidase but also other enzymes such as beta-glucosidase, alkaline phosphatase, lactose dehydrogenase, sulfatase, and horseradish peroxidase.Synthetic betaine-type metabolite analogs possess practicable cryoprotective ability for various enzymes, and are considerably superior to previously reported cryoprotectants.
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Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size 査読あり 国際誌
Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
BIOCONJUGATE CHEMISTRY 26 ( 8 ) 1775 - 1781 2015年8月
出版者・発行元：AMER CHEMICAL SOC
The design and synthesis of materials capable of activating the immune system in a safe manner is of great interest in immunology and related fields. Lactobacilli activate the innate immune system of a host when acting as probiotics. Here, we constructed lactobacilli-mimicking materials in which polysaccharide peptidoglycan complexes (PS PGs) derived from lactobacilli were covalently conjugated to the surfaces of polymeric microparticles with a wide variety of sizes, ranging from 200 nm to 3 mu m. The artificial lactobacilli successfully stimulated macrophages without cytotoxicity. Importantly, we found that the size of artificial lactobacilli strongly influenced their immunostimulating activities, and that artificial lactobacilli of 1 mu m exhibited 10-fold higher activity than natural lactobacilli. One major advantage of the artificial lactobacilli is facile control of size, which cannot be changed in natural lactobacilli. These findings provide new insights into the design of materials for immunology as well as the molecular biology of lactobacillus.
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Establishment of a macrophage-activating factor (MAF) assay system using human-monocytic cell line, THP-1. 査読あり
Inoue T., Ishikawa M., Sumiya Y., Kohda H, Inui T., Kuchiike D., Kubo K., Uto Y and Nishikata T.
Anticancer Research 35 4441 - 4445 2015年8月
共著
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Oral Colostrum Macrophage-activating Factor for Serious Infection and Chronic Fatigue Syndrome: Three Case Reports. 査読あり
Inui T., Kubo K., Kuchiike D., Uto Y., Nishikata T., Sakamoto N., Mette M.
Anticancer Research 35 ( 8 ) 4545 - 4549 2015年8月
共著
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Macrophage Activation Mechanisms in Human Monocytic Cell Line-derived Macrophages. 査読あり
Sumiya Y, Ishikawa M, Inoue T, Inui T, Kuchiike D, Kubo K, Uto Y, Nishikata T
Anticancer research 35 ( 8 ) 4447 - 4451 2015年8月
共著
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Establishment of a Macrophage-activating Factor Assay System Using the Human Monocytic Cell Line THP-1 査読あり
Takahiro Inoue, Mami Ishikawa, Yu Sumiya, Haruka Kohda, Toshio Inui, Daisuke Kuchiike, Kentaro Kubo, Yoshihiro Uto, Takahito Nishikata
ANTICANCER RESEARCH 35 ( 8 ) 4441 - 4445 2015年8月
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出版者・発行元：INT INST ANTICANCER RESEARCH
Background: As the mechanism of macrophage activation is not well-understood, standardization of an assay system for measuring phagocytic activities of macrophages will be useful for research on macrophages. Previously, we established a novel standardized macrophage-activating factor (MAF) assay system using U937. Materials and Methods: Using the human monocytic cell line THP-1, another standardized MAF assay system was established. Characteristic gene expression of U937- and THP-1-derived macrophages was compared by gene expression microarray analysis. Results: Both U937- and THP-1-derived macrophages showed obvious phagocytic activities with unique characteristics and, therefore, could not be assigned to a single sub-type. Conclusion: Activation of macrophages is an intricate cellular process. Comparison of our two novel assay systems provides new insights into macrophage activation mechanisms.
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Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size 査読あり
Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, and Jun Matsui
Bioconjugate Chemistry 26 1775 - 1781 2015年8月
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担当区分：筆頭著者
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Effects of background anionic compounds on the activity of the hammerhead ribozyme in Mg2+-unsaturated solutions 査読あり
S. Nakano, Y. Kitagawa, D. Miyoshi, and N. Sugimoto
J. Biol. Inorg. Chem 20 ( 6 ) 1049 - 1058 2015年7月
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Effects of cosolvents on the folding and catalytic activities of the hammerhead ribozyme 査読あり
S. Nakano, Y. Kitagawa, H. Yamashita, D. Miyoshi, and N. Sugimoto
ChemBioChem 16 ( 12 ) 1803 - 1810 2015年7月
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生命分子－合成高分子ハイブリッドの開発とナノバイオマテリアル，その可能性 招待あり
長濱宏治
Materials Stage 7 18 - 21 2015年7月
単著
担当区分：筆頭著者
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Anticancer drug-based multifunctional nanogels through self-assembly of dextran-curcumin conjugates toward cancer theranostics 査読あり
Koji Nagahama, Yoshinori Sano, Takayuki Kumano
Bioorganic & Medical Chemistry Letters 25 2519 - 2522 2015年6月
共著
担当区分：筆頭著者
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Effects of four-week feed restriction on toxicological parameters in beagle dogs. 査読あり
Takamatsu K., Yamashita H., Satake S., Kazusa K., Tabata H., and Nishikata T.
Experimental Animals 64 269 - 280 2015年5月
共著
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A mRNA-responsive G-quadruplex-based drug release system 査読あり
H. Yaku, T. Murashima, D. Miyoshi, N. Sugimoto
Sensors 15 ( 4 ) 9388 - 9403 2015年4月
共著
DOI： 10.3390/s150409388
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Label and Label-Free Detection Techniques for Protein Microarrays 査読あり 国際共著
Amir Syahir, Kenji Usui, Kin-ya Tomizaki, Kotaro Kajikawa, Hisakazu Mihara
Microarrays 4 ( 4 ) 228 - 244 2015年4月
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DNA G-quadruplex detection system employing a protein fibril ligand 査読あり
R. Maeda, H. Yaku, T. Nakabayashi, T. Murashima, N. Sugimoto, D. Miyoshi
Telomere and Telomerase 2 2015年3月
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Mechanistic study of unusual enzyme activation caused by addition of inert betaine-type metabolite and the analogs
Yuichi Nakagawa, Kazuya Koumoto
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 249 2015年3月
出版者・発行元：AMER CHEMICAL SOC