論文
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Self-Assembling Polymer Micelle/Clay Nanodisk/Doxorubicin Hybrid Injectable Gels for Safe and Efficient Focal Treatment of Cancer 査読あり
Koji Nagahama, Daichi Kawano, Naho Oyama, Ayaka Takemoto, Takayuki Kumano, Junji Kawakami
BIOMACROMOLECULES 16 ( 3 ) 880 - 889 2015年3月
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出版者・発行元：AMER CHEMICAL SOC
The purpose of this study was to fabricate a safe and effective doxorubicin (DOX)-delivery system for focal cancer chemotherapy. A novel biodegradable injectable gel was developed through self-assembly of poly(D,L-lactide-co-glycolide)-b-poly(ethylene glycol)-b-poly(D,L-lactide-co-glycolide) (PLGA-PEG-PLGA) copolymer micelles, clay nanodisks (CNDs), and DOX. We discovered that DOX loaded in the hybrid gels acts as an anticancer drug and as a building block to organize new gel networks. Accordingly, long-term sustained release of DOX from hybrid injectable gels without initial burst release was achieved. Moreover, it was revealed that the DOX incorporated into gel networks controls its own release profile. This hybrid injectable gel is a self-controlled drug release system, which is a novel concept in controlled drug release. Importantly, a single injection of PLGA-PEG-PLGA/CND/DOX hybrid gel provides long-term sustained antitumor activity in vivo against human xenograft tumors in mice, suggesting the potential of hybrid gels as a valuable local DOX-delivery platform for cancer focal therapy.
DOI： 10.1021/bm5017805
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Site-Specific Mineralization of Silica and Calcium on DNAs Using a Designed Peptide 査読あり
K. Usui, H. Nishiyama, A. Yamada, M. Ozaki, T. Tsuruoka, K.-y. Tomizaki
Peptide science 2014 325 - 326 2015年3月
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担当区分：筆頭著者
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Self-Assembling Polymer Micelle/Clay Nanodisk/Doxorubicin Hybrid Injectable Gels for Safe and Efficient Focal Treatment of Cancer 査読あり
Koji Nagahama, Daichi Kawano, Naho Oyama, Ayaka Takemoto, Takayuki Kumano, Junji Kawakami
Biomacromolecules 16 880 - 889 2015年3月
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担当区分：筆頭著者
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Synthesis and lmmunestimulating Activity of Lactobacilli-Originated Polysaccharide-Polymeric Microparticle Conjugates 査読あり 国際誌
Koji Nagahama, Takayuki Kumano, Tsubasa Nakata, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
LANGMUIR 31 ( 4 ) 1489 - 1495 2015年2月
出版者・発行元：AMER CHEMICAL SOC
The design and synthesis of biomaterials capable of activating the immune system are of interest in immunology-related fields because of their ability to tune up the immune defenses of the host. Lactobacilli are a major constituent of normal human indigenous flora, and some specific strains are known to activate the immune system of the host as probiotics. In this study, we first fabricated novel biohybrid materials in which lactobacilli (L. casei strain Shirota, LcS)-originated polysaccharide-peptidoglycan complexes (PS-PGs) are conjugated with polymeric microparticles (MPs). PS-PGs conjugated onto polymeric MPs surfaces bound its specific antibody, suggesting that PS-PGs kept their original molecular recognition ability. The PS-PGs-based hybrid MPs with an appropriate density of conjugated PS-PGs effectively induced high levels of IL-12 production from macrophages without cytotoxicity. These results suggest that LcS-originated PS-PGs could be available bio-originated materials for developing novel biomaterials capable of activating the immune system in a safe manner.
DOI： 10.1021/la5041757
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Synthesis and Immunestimulating Activity of Lactobacilli-Originated Polysaccharide-Polymeric Microparticle Conjugates 査読あり
Koji Nagahama, Takayuki Kumano, Tsubasa Nakata, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, and Jun Matsui
Langmuir 31 1489 - 1495 2015年1月
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担当区分：筆頭著者
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Synthesis and Immunestimulating Activity of Lactobacilli-Originated Polysaccharide-Polymeric Microparticle Conjugates 査読あり
Koji Nagahama, Takayuki Kumano, Tsubasa Nakata, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
Langmuir 31 1489 - 1495 2015年
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担当区分：筆頭著者
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Cryoprotective ability of betaine-type metabolite analogs during freezing denaturation of enzymes 査読あり
Yuichi Nakagawa, Masahiro Sota, Kazuya Koumoto
Biotechnology Letters 37 1607 - 1613 2015年
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担当区分：筆頭著者
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化学修飾技術を利用した乳酸菌模倣粒子による免疫賦活 招待あり
甲元一也、長濱宏治、松井淳
バイオインダストリー 32 ( 9 ) 42 - 47 2015年
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担当区分：筆頭著者
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Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size 査読あり
Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
Bioconjugate Chemistry 26 ( 8 ) 1775 - 1781 2015年
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担当区分：筆頭著者
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Significance of anionic functional group in betaine-type metabolite analogs on the facilitation of enzyme reactions 国際誌
Yuichi Nakagawa, Kotomi Takagi, Ryutaro Genjima, Kazuya Koumoto
Bioprocess and Biosystems Engineering 38 ( 9 ) 1811 - 1817 2015年
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Enhanced Chromogenic Sensitivity of Horseradish Peroxidase-catalyzed Oxidative Reactions in the Presence of Betaine-type Metabolite Analogs 査読あり
Kotomi Takagi, Yasuhiro Kashima, Satoshi Fujii, Kazuya Koumoto
Bulletin of the Chemical Society of Japan 88 ( 8 ) 1074 - 1082 2015年
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DNA structures under molecular crowding conditions with a phosphorylcholine derivative (MPC) 査読あり
Yu-mi Ueda, Tomohiro Konno, Kazuhiko Ishihara, Naoki Sugimoto, Daisuke Miyoshi
Transact. Mat. Res. Soc. Japan 40 99 - 102 2015年
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担当区分：筆頭著者
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Effects of background anionic compounds on the activity of the hammerhead ribozyme in Mg2+-unsaturated solutions 査読あり
Shu-ichi Nakano, Yuichi Kitagawa, Daisuke Miyoshi, Naoki Sugimoto
J. Biol. Inorg. Chem. 20 1049 - 1058 2015年
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Stabilization of DNA Structures With Poly(ethylene sodium phosphate) 査読あり
R. Moriyama, Y. Iwasaki, D. Miyoshi
J. Phys. Chem. B 119 11969 - 11977 2015年
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Effects of cosolvents on the folding and catalytic activities of the hammerhead ribozyme 査読あり
S. Nakano, Y. Kitagawa, H. Yamashita, D. Miyoshi, N. Sugimoto
ChemBioChem 16 1803 - 1810 2015年
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A mRNA-responsive G-quadruplex-based drug release system 招待あり 査読あり
Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, and Naoki Sugimoto
Sensors 15 ( 4 ) 9388 - 9403 2015年
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担当区分：筆頭著者
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DNA G-quadruplex detection system employing a protein fibril ligand 招待あり 査読あり
Ryuichi Maeda, Hidenobu Yaku, Takakazu Nakabayashi, Takashi Murashima, Naoki Sugimoto, Daisuke Miyoshi
Telomere and Telomerase 2 e691 2015年
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担当区分：筆頭著者
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DNA structures under molecular crowding conditions with a phosphorylcholine derivative (MPC)
Yu-mi Ueda, Tomohiro Konno, Kazuhiko Ishihara, Naoki Sugimoto, Daisuke Miyoshi
Transact. Mat. Res. Soc. Japan 40 99 - 102 2015年
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Stabilization of DNA Structures With Poly(ethylene sodium phosphate)
R. Moriyama, Y. Iwasaki, D. Miyoshi
J. Phys. Chem. B 119 11969 - 11977 2015年
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Metal nanocrystal/metal-organic framework core/shell nanostructure from selective self-assembly induced by localization of metal ion precursors on nanocrystal surface 査読あり
T. Ohhashi, T. Tsuruoka*, T. Matsuyama, Y. Takashima, H. Nawafune, H. Minami, K. Akamatsu
J. Colloid Interface Sci 451 212 - 215 2015年
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