論文
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DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity 査読あり
Kenji Usu, Arisa Okada, Shungo Sakashita, Masayuki Shimooka, Takaaki Tsuruoka, Shu-ichi Nakano, Daisuke Miyoshi, Tsukasa Mashima, Masato Katahira and Yoshio Hamada
Molecules 22 1991 2017年11月
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Influence of intracellular environment on allosteric ribozyme activity
Misaki Kameno, Mika Sawada, Nae Sakimoto and Junji Kawakami
Proc. 44th Intl. Symp. Nucleic Acid Chemistry 178 - 179 2017年11月
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担当区分：筆頭著者
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Quantification of stabilization effect of co-solutes on RNA tertiary interaction
Natsumi Sasaki, Daisuke Miyoshi and Junji Kawakami
Proc. 44th Intl. Symp. Nucleic Acid Chemistry 174 - 175 2017年11月
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担当区分：筆頭著者
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Non-Covalent Loading of Anti-Cancer Doxorubicin by Modularizable Peptide Self-Assemblies for a Nanoscale Drug Carrier 査読あり
Kin-ya Tomizaki, Kohei Kishioka, Shunsuke Kataoka, Makoto Miyatani, Takuya Ikeda, Mami Komada, Takahito Imai, Kenji Usui
Molecules 22 ( 11 ) 1916 2017年11月
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細胞環境化学模倣実験系におけるDNAの構造とその熱力学的安定性
三好大輔
日本核酸化学会誌 1 13 - 19 2017年10月
単著
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Newly characterized interaction stabilizes DNA structure: oligoethylene glycols stabilize G-quadruplexes CH–π interactions 査読あり
H. Tateishi-Karimata, T. Ohyama, T. Muraoka, P. Podbevsek, A. M. Wawro, S. Tanaka, S. Nakano, K. Kinbara, J. Plavec, N. Sugimoto
Nucleic Acids Res. 45 7021 - 7030 2017年7月
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Fluorescent and Luminescent Fusion Proteins for Analyses of Amyloid Beta Peptide Aggregation 査読あり
Usui K, Mie M, Andou T, Mihara H, Kobatake E.
J Pept Sci. 23 ( 7-8 ) 659 - 665 2017年7月
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Quantitative relationship between chemical properties and bioactivities of anti-microRNA oligonucleotides targeted to tumor-associated microRNA-21 査読あり
Koji Nagahama, Kenta Iseda, Daichi Kawano, Junji Kawakami
BIOCHIMIE 137 124 - 131 2017年6月
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担当区分：最終著者, 責任著者 出版者・発行元：ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Synthetic anti-microRNA oligonucleotides (AMOs) are promising drug candidates to inactivate disease related microRNAs because of their sequence-specific binding to their targets and the variety of chemical modifications available. Over the last decade, the qualitative relationships between the chemical properties of AMOs and bioactivity (inactivation of their target miRNAs) have been studied to enhance their bioactivity. On the other hand, in real-world drug development, drugs must be designed case-by case, taking many factors into account. Thus, in order to design AMOs that target specific miRNA, understanding the quantitative relationship between the chemical properties of AMOs and inactivation of their target miRNA is necessary. Here, we aimed to find the specific quantitative relationship of AMOs targeted to tumor-associated miR-21 through direct comparison of their inactivation efficacies with systematically varied chemical properties, including sequence-specific binding affinity, nuclease resistance, and RNase H activation. As a result, we newly found the quantitative relationships; (1) sequence specific binding affinity of AMOs against miR-21 is the main determining factor for inactivation efficacy, (2) nuclease resistance of AMOs impacts their miR-21 inactivation efficacy acting cooperatively with the binding affinity, although nuclease resistance alone does not affect the miRNA inactivation efficacy, and (3) RNase H activation is unnecessary. This study also demonstrates the utility of the obtained relationship for the design of AMO-based drugs targeted to miR-21, through cell-based analyses. Thus, the obtained quantitative relationship would make it possible to predict the miR-21 inactivation efficacy of AMOs which are newly designed. (C) 2017 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
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Quantitative relationship between chemical properties and bioactivities of anti-microRNA oligonucleotides targeted to tumor-associated microRNA-21 査読あり
Koji Nagahama, Kenta Iseda, Daichi Kawano, Junji Kawakami
Biochimie 137 124 - 131 2017年6月
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担当区分：筆頭著者
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NF-kappaB prevents oncogenic Ras-induced b-actin cleavage in p53-deficient cells 査読あり
Wataru Sugimoto, Katsuhiko Itoh, Toshiya Kotari, Alvin K. Guo,Takahiro Ebata, Hiroaki Hirata, Keiko Kawauchi
International Journal of Cancer & Cellular Biology Research 2 ( 1 ) 15 - 18 2017年5月
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Highly Sensitive Telomerase Assay Insusceptible to Telomerase and Polymerase Chain Reaction Inhibitors for Cervical Cancer Screening Using Scraped Cells. 査読あり
Hidenobu Yaku , Yoshio Yoshida, Hidehiko Okazawa, Yasushi Kiyono, Yuko Fujita, Daisuke Miyoshi
Anal. Chem. 89 ( 13 ) 6948 - 6953 2017年5月
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PEG-based nanocomposite hydrogel: Thermoresponsive sol-gel transition controlled by PLGA-PEG-PLGA molecular weight and solute concentration 査読あり
Makoto Miyazaki, Tomoki Maeda, Kenji Hirashima, Naruki Kurokawa, Koji Nagahama, Atsushi Hotta
Polymer 115 246 - 254 2017年4月
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担当区分：筆頭著者
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Quantitative relationship between chemical properties and bioactivities of anti-microRNA oligonucleotides targeted to tumor-associated microRNA-21 査読あり
Koji Nagahama, Kenta Iseda, Daichi Kawano, Junji Kawakami
Biochimie 137 124 - 131 2017年3月
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担当区分：筆頭著者
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Atsushi Ikeda, Tatsuya Iizuka, Naotake Maekubo, Kazuyuki Nobuswa, Kouta Sugikawa, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Motofusa Akiyama
Chemistry an Asian Journal 12 ( 10 ) 1069 - 1074 2017年3月
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Development of Peptide Microarray to Detect the Protein in Saliva for Periodontal Disease Test 査読あり
Yuki Tominaga, Kenji Usui, Akiyoshi Hirata, Atsushi Kitagawa, Hiro-O Ito and Kiyoshi Nokihara
Peptide Science 2016 131 - 132 2017年3月
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Motofusa Akiyama, Kouta Sugikawa, Kazuya Koumoto, Yua Kashijima, Jiawei Li, Toshio Suzuki, Takeshi Nagasaki, Atsushi Ikeda
Organic & Biomolecular Chemistry 15 ( 9 ) 1990 - 1997 2017年2月
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Cell and Molecular Mechanics in Health and Disease 招待あり 査読あり
Keiko Kawauchi, Hideaki Fujita, Daisuke Miyoshi, Evelyn K. F. Yim, and Hiroaki Hirata
BioMed Research International Article ID 2860241 2017年2月
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DOI： 10.1155/2017/2860241
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Nicotine-Selective Polymeric Adsorbent Obtained by Molecular Imprinting with Excess Use of Itaconic Acid 査読あり
Haruka Nogami, Yuma Nakahori, Takashi Murashima, Jun Matsui
Chromatography 38 15 - 21 2017年1月
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Substrate stiffness influences doxorubicin- induced p53 activation via ROCK2 expression 査読あり
Ebata T, Mitsui Y, Sugimoto W, Maeda M, Araki K, Machiyama H, Harada I, Sawada Y, Fujita H, Hirata H, Kawauchi K
BioMed Research International 2017年1月
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Unexpected Position-Dependent Effects of Ribose G‑Quartets in G‑Quadruplexes 査読あり
J. Zhou, S. Amrane, F. Rosu, G.F. Salgado, Y. Bian, H. Tateishi-Karimata, E. Largy, D. N. Korkut, A. Bourdoncle, D. Miyoshi, J. Zhang, H. Ju, W. Wang, N. Sugimoto, V. Gabelica, and J. Mergny
J. Am. Chem. Soc. 139 7768 - 7779 2017年
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