Review Papers (Misc) -
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Peptide microarrays using structure-based peptide libraries for protein chips
K Usui, M Takahashi, T Ojima, M Suzuki, K Nokihara, F Tamiya, H Mihara
BIOPOLYMERS 71 ( 3 ) 403 - 403 2003
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS INC
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プロテインチップ Reviewed
臼井 健二, 三原 久和
化学装置2003年4月号 36 - 38 2003
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Construction of Protein-Detection Microarrays with FRET Fingerprint Patterns Using an α-Helical Peptide Library. Reviewed
臼井健二, 高橋瑞稀, 軒原清史, 三原久和
生体機能関連化学シンポジウム講演要旨集 18th 2003
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Polysaccharide-polynucleotide interaction (XI); Novel separation system of RNAs by using Schizophyllan-appended column
T Kimura, K Koumoto, M Mizu, K Sakurai, S Shinkai
CHEMISTRY LETTERS ( 12 ) 1240 - 1241 2002.12
Publisher：CHEMICAL SOC JAPAN
Schizophyllan belongs to the beta-1,3-glucan family and can form a complex with particular polynucleotides.(1) We applied this complexation to specifically separate polynucleotides by constructing a schizophyllan-appended column. The column trapped poly(C) and poly(A), while it did not show any affinity to poly(G), which is consistent with the experimental results obtained in the homogenous solution. The present results indicate that the s-SPG column can be a new separation device for biomolecules.
DOI： 10.1246/cl.2002.1240
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ホヤRNAiプロトコル
藤原滋樹，西方敬人，菅沼一樹，佐藤愛，浦野亜規，片野美緒
細胞工学 21 771 - 777 2002.11
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：秀潤社
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「生命を化学する」・・・生命科学のすすめ 生命現象を化学に活用する
中野修一・杉本直己
化学 57 ( 11 ) 42 - 47 2002.11
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：化学同人
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Gel formation properties of a uracil-appended cholesterol gelator and cooperative effects of the complementary nucleobases
E Snip, K Koumoto, S Shinkai
TETRAHEDRON 58 ( 43 ) 8863 - 8873 2002.10
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
We designed and synthesized a uracil-appended cholesterol gelator (1) in order to control the gel stability and the gel morphology by addition of the complementary and non-complementary nucleobase derivatives. Compound 1 forms columnar stacks in cyclohexane due to the van der Waals interaction (cholesterol-cholesterol interaction) and the intergelator hydrogen bonding between uracil moieties. Addition of a 'monomeric' adenosine (3) into the gel only decreases the stability with increasing the concentration. The destabilization is ascribed to a lack of intergelator hydrogen bonding accompanied with forming the complementary base pairs between 1 and 3. In contrast, addition of adenine-appended cholesterol (7) induces a different behavior; with increasing 7 concentration the mixed gel is initially stabilized and then destabilized, giving rise to a maximum at the ratio of 1/7= 1:1 for the T-gel plot. One may consider, therefore, that when the additive has a common, column-forming cholesterol moiety, the cholesterol-cholesterol interaction can operate cooperatively with the complementary base pairing. In addition, the gel fiber structure is clearly changed by the addition of 7. Taking the fact that there is no report for such an additive effect inducing a structural change with maintaining the gel stability into consideration, our attempt combining cholesterol columnar stacks with the nucleobase additives provides a new methodology to control the stability and the morphology of organogels. (C) 2002 Elsevier Science Ltd. All rights reserved.
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Polysaccharide-polynucleotide complexes VIII. Cation-induced complex formation between polyuridylic acid and schizophyllan
K Sakurai, R Iguchi, K Koumoto, T Kimura, M Mizu, Y Hisaeda, S Shinkai
BIOPOLYMERS 65 ( 1 ) 1 - 9 2002.10
Publisher：JOHN WILEY & SONS INC
Schizophyllan belongs to the beta-1,3-D-glucan family, and dissolves as a single chain in dimethylsulfoxide (DMSO) and as a triple helix in water, respectively. It is already known that when we prepare a mixture of poly(C) and schizophyllan in DMSO and subsequently exchange the solvent to water, poly(C) anti schizophyllan can form a complex. When we applied this procedure to the poly(U) system, we could not induce the complex formation between poly(U) and schizophyllan at all. However, we found that addition of some alkaline cations is necessary to induce the complexation between poly(U) and schizophyllan. The complex stability strongly depends on both the cation species and the salt concentration. The complexation is sensitively reflected in the CD spectrum. The magnitude of the spectral change is followed by the order; Rb+ much greater than K+ > Na+ ? Cs+. This cation
DOI： 10.1002/bip.10189
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Chemical modification of curdlan: Characteristic complexation with polynucleotides by control of the molecular weight.
K Koumoto, K Sakurai, S Shinkai, T Kunitake
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 224 ( 2 ) U429 - U429 2002.8
Publishing type：Research paper, summary (international conference) Publisher：AMER CHEMICAL SOC
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Novel DNA-polysaccharide triple helix and its application to a gene carrier.
K Sakurai, S Shinkai
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 224 ( 2 ) U476 - U477 2002.8
Publishing type：Research paper, summary (international conference) Publisher：AMER CHEMICAL SOC
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「生命を化学する」・・・小分子で巨大タンパク質の挙動を操る タンパク質のドメイン・スワッピングとその制御
中野修一・杉本直己
化学 57 ( 8 ) 42 - 47 2002.8
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：化学同人
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Molecular design of "super" hydrogelators: Understanding the gelation process of azobenzene-based sugar derivatives in water
H Kobayashi, A Friggeri, K Koumoto, M Amaike, S Shinkai, DN Reinhoudt
ORGANIC LETTERS 4 ( 9 ) 1423 - 1426 2002.5
Publisher：AMER CHEMICAL SOC
[GRAPHIC]
As an attempt to rationally design aqueous organogelators, a bolaamphiphilic azobenzene derivative (1) bearing two sugar groups was synthesized. Compound 1 formed a gel in water even at concentrations as low as 0.05 wt% (0.65 mM). Spectroscopic studies and electron-micrographic observations have clarified the gel structure and the origin of the gelation ability for water.DOI： 10.1021/ol025519+
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アルツハイマー病とNO・金属イオンの化学
藤井敏司，杉本直己
化学 57 ( 4 ) 43 - 47 2002.3
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：化学同人
アルツハイマー病と一酸化窒素，金属イオンの関わりについて，総合的に論じた
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核酸の相互作用
杉本直己・中野修一
化学フロンティア5 生命化学のニューセントラルドグマ テーラーメイド・バイオケミストリーのめざすもの 24 - 34 2002.2
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：化学同人
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狂牛病・プリオン病と金属イオンの化学
藤井敏司，杉本直己
化学 57 ( 3 ) 30 - 34 2002.2
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：化学同人
狂牛病に代表されるプリオン病と金属イオンの関係について総合的に解説した
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Polysaccharide-polynucleotide complexes. Part 12. Enhanced affinity for various polynucleotide chains by site-specific chemical modification of schizophyllan
K Koumoto, T Kimura, M Mizu, T Kunitake, K Sakurai, S Shinkai
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 ( 22 ) 2477 - 2484 2002
Publisher：ROYAL SOC CHEMISTRY
Recently, we found that schizophyllan (SPG), which is a natural and neutral polysaccharide, can form macromolecular complexes with certain polynucleotides. In order to increase the stability and the affinity of the complexes, we synthesized a 2-aminoethanol-appended SPG (N-s-SPG) by utilizing periodate oxidation and reductive amination. Although the modi. cation level was only 2.4 +/- 0.3%, the melting temperature of the poly(C)-N-s-SPG complex was increased by 8 degreesC comparing with the poly(C)-s-SPG complex. In addition, the conformation of the complex was scarcely perturbed by the introduction of amino groups. When we formed complexes of N-s-SPG and other polynucleotides, the melting temperature of their complexes increased without exception. Moreover, the amino modification induced complexation with poly(U), which does not form a complex with SPG. The affinity for the short chain nucleotide was also enhanced. These results indicate that the present modi. cation method is quite useful to improve complex stability and we believe, therefore, that this strategy will make it possible to apply SPG to a novel gene carrier.
DOI： 10.1039/b207553a
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Sol-gel phase transition induced by fiber-vesicle structural changes in sugar-based bolaamphiphiles
H Kobayashi, K Koumoto, JH Jung, S Shinkai
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2 ( 11 ) 1930 - 1936 2002
Publisher：ROYAL SOC CHEMISTRY
The research objects of this paper were to design bolaamphiphilic gelators utilizing a sugar family as a source of solvophilic groups and an azobenzene segment as a solvophobic group and to monitor the aggregation mode utilizing the spectroscopic properties of the azobenzene chromophore. The results indicated that the bolaamphiphiles act, although only for specific DMSO-water mixtures, as gelators and form a unique supramolecular helical structure in the gel phase. The UV- Vis and CD spectra showed that the azobenzene segments adopt H- type face- to- face orientation and the dipole moments are arranged in the right- handed (R)- helicity. Since the fibrils as observed by electron microscope possess the right- handed helical structure, one may consider that the microscopic azobenzene-azobenzene orientation is reflected by the macroscopic supramolecular structure. When boronic acid- appended poly( L-lysine) was added, the gel phase was changed into the sol phase in the macroscopic level and the fibrous aggregate was changed into the vesicular aggregate in the microscopic level. These changes, which are usually induced by a temperature change, are due to the specific boronic acid- sugar interaction occurring at the constant temperature. Interestingly, when -fructose which shows high affinity with the boronic acid group was added, the sol phase and the vesicular aggregate were changed back to the gel phase and the fibrous aggregate, respectively. This means that the phase and morphological changes in the sugar- integrated bolaamphiphiles can be controlled reversibly.
DOI： 10.1039/b204631h
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Molecular Imprinting in Alcohols: utility of a pre-polymer based strategy for synthesizing stereoselective artificial receptor polymers in media hydrophilic
Anal.Chim.Acta 466, 11-15 2002
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ホヤのRNAiプロトコル
西方 敬人
細胞工学 21, 771-777 2002