Review Papers (Misc) -
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ラーニング・アシスタント活⽤事例紹介と ラーニング・アシスタント経験学⽣へのアンケート調査
臼井 健二
甲南大学全学共通教育センター紀要 3 191 - 196 2025.3
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Diversity of CAR-cell therapies in cancer treatment and their future approach Reviewed
Shiori Degawa, Keiko Kawauchi, Takahito Nishikata
Journal of the Society of Japanese Women Scientists 25 43 - 48 2025.2
Authorship：Last author, Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
DOI： https://doi.org/10.5939/sjws.250008
DOI： https://doi.org/10.5939/sjws.250008
Other Link： https://www.jstage.jst.go.jp/browse/sjws/25/0/_contents/-char/en
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ナノバイオ分野におけるマイクロ波の影響に関する研究と産業応用を目指した技術開発
栢森 史浩、有本 米次郎、中西 伸浩、臼井 健二
マイクロ波の工業応用事例集 345 - 349 2025.2
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Three- and four-stranded nucleic acid structures and their ligands Reviewed
6 466 - 491 2025.2
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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化学大家 川本幸民 Invited
藤井敏司
和光純薬時報 93 ( 1 ) 20 - 23 2025.1
Authorship：Lead author, Last author, Corresponding author Publishing type：Article, review, commentary, editorial, etc. (other)
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がん免疫療法におけるCAR細胞療法の多様性とその展望 Reviewed
出川詩織、川内敬子、西方敬人
日本女性科学者の会学術誌 25 43 - 48 2025.1
Authorship：Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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ナノバイオ分野におけるマイクロ波の影響に関する研究 Reviewed
栢森 史浩, 中西 伸浩, 臼井 健二
色材協会誌 97 ( 6 ) 156 - 159 2024.6
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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ナノバイオ分野におけるマイクロ波の影響に関する研究 Invited Reviewed
栢森 史浩、中西 伸浩、臼井 健二
色材協会誌 97 ( 6 ) 156 - 159 2024.6
Authorship：Lead author, Last author
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日本酒麹菌産生物質デフェリフェリクリシンががん細胞に及ぼす効果について Invited
月生雅也、取井猛流、木下菜月、戸所健彦、石田博樹、西方敬人、川内敬子
食と医療 29 14 - 20 2024.4
Authorship：Last author, Corresponding author Publishing type：Article, review, commentary, editorial, etc. (other)
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細胞内環境で安定化する核酸構造を標的にした低分子薬の開発 Reviewed
橋本 佳樹, 川内 敬子, 三好 大輔
MEDCHEM NEWS 34 36 - 42 2024.4
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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タンパク質の構造や酵素反応へのマイクロ波の影響 Reviewed
栢森 史浩、平尾 莞、鶴岡 孝章、中西 伸浩、臼井 健二
日本電磁波エネルギー応用学会論文誌 7 1 - 9 2023.12
Authorship：Lead author, Corresponding author
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RNA高次構造を標的とした光線力学的療法の展望 Invited
BIO Clinica 38 ( 13 ) 76 - 78 2023.11
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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水和を介して酵素の活性化、安定化を引き起こす低分子双性イオン性分子の開発 Invited
甲元一也
野口研究所時報 ( 66 ) 3 - 9 2023.10
Authorship：Lead author, Corresponding author Publishing type：Article, review, commentary, editorial, etc. (other)
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様々な分野に適用可能な皮膚感作性試験法の開発
臼井健二, 宮崎洋, 山下邦彦, 栢森史浩, 有海秀人
アレルギーの臨床 ( 11月 ) 2023.10
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様々な分野に適用可能な皮膚感作性試験法の開発
臼井健二、宮﨑 洋、山下 邦彦、栢森 史浩、有海秀人
アレルギーの臨床 2023.10
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核酸医療の基礎知識 修飾核酸 Invited
秋田 智香、冨田 恵麗沙、川上 純司
月刊 臨床神経科学 41 ( 5 ) 639 - 642 2023.5
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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Effects of structural selective G4 ligand on telomere length and TERRA expression level
橋本佳樹, 取井猛流, 木下菜月, 川内敬子, 建石寿枝, 杉本直己, 杉本直己, 三好大輔
日本化学会春季年会講演予稿集(Web) 103rd 2023
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Nucleic Acids under Multimolecular Crowding Biosystems
建石寿枝, 三好大輔, 杉本直己, 杉本直己
CSJ Current Review ( 45 ) 2023
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日本発の核酸医薬を世界に向けて
川上純司
SCAS NEWS 2023-1 1 - 2 2023
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LC/MSを使用したオリゴ核酸の不純物分析
瀬崎浩史、内藤厚子、林明生、川上純司、井上貴雄、山口卓男、小比賀聡
Medical Science Digest 49 ( 14 ) 780 - 782 2023
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Shape control of Au nanostructures using peptides for biotechnological applications Reviewed
Shuhei Yoshida, Kin-ya Tomizaki and Kenji Usui
Chemical Communications, in press 2023
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タンパク質の構造や酵素反応へのマイクロ波の影響 Reviewed
栢森史浩、平尾莞、鶴岡孝章、中西伸浩、臼井健二
日本電磁波エネルギー応用学会論文誌 2023
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細胞架橋ゲルの開発および再生医療応用 Invited Reviewed
上田菜摘美、長濱宏治
バイオマテリアル－生体材料－ 41 ( 3 ) 244 - 245 2023
Authorship：Last author, Corresponding author
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Elucidation of the mechanism of nuclear actin filament formation by anti-tumor drug and its regulation
取井猛流, 杉本亘, 伊藤功彦, 木下菜月, 月生雅也, 後藤俊志, 上原郁野, 中嶋亘, BUDIRAHARDJA Yemima, 三好大輔, 西方敬人, 田中信之, 平田宏聡, 川内敬子
日本分子生物学会年会プログラム・要旨集(Web) 46th 2023
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Regulation for expression of cancer-related genes by cytosine-quadruplexes
建石寿枝, 川内敬子, 杉本直己, 杉本直己
日本核酸医薬学会年会講演要旨集(CD-ROM) 8th 2023
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Effects of structural selective G4 ligand on telomere length and TERRA expression level
橋本佳樹, 取井猛流, 木下菜月, 川内敬子, 建石寿枝, 杉本直己, 杉本直己, 三好大輔
日本化学会春季年会講演予稿集(Web) 103rd 2023
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核酸医薬の効果に影響する細胞内物質のin vitro評価 Invited
中野修一
Precision Medicine 5 ( 14 ) 64 - 67 2022.11
Authorship：Lead author, Last author, Corresponding author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：北隆館
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血液検査によるアルツハイマー病早期発症リスク評価を可能とする電気化学センサの開発 Invited
藤井敏司
BIO INDUSTRY 39 ( 10 ) 13 - 17 2022.10
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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製造委託の際に知っておきたい核酸医薬の特性ー品質と安全性評価面を中心に Invited
川上 純司
Pharm Tech Japan 38 ( 12 ) 2017 - 2022 2022.9
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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細胞内環境で安定化される核酸非構造を狙った分子標的薬の開発 Invited
川内敬子・橋本佳樹・杉本 渉・三好大輔
バイオマテリアル－生体材料 40 ( 3 ) 206 - 211 2022.8
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ペプチドを用いたアレルギー感作性試験の開発および，ペプチド合成を学生実験に導入した試み
Peptide Newsletter Japan,
臼井健二 125 2022.7
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「受動的な学び」から「能動的な学び」へ 〜高大連携活動の展開と可能性〜 Invited
甲元一也
月刊「兵庫教育」 74 ( 3 ) 4 - 7 2022.6
Authorship：Lead author, Corresponding author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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Droplet degradation targeted at secondary structure of nucleic acid
鶴田充生, 取井猛流, 小畠一起, 川内敬子, 建石(狩俣)寿枝, 杉本直己, 三好大輔
日本化学会春季年会講演予稿集(Web) 102nd 2022
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A Novel G4 Ligand Screening System with G4-selective Fluorescent Probe
橋本佳樹, 取井猛流, 藤田ひな, 川内敬子, 建石寿枝, 杉本直己, 三好大輔
日本化学会春季年会講演予稿集(Web) 102nd 2022
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The role of G-quadruplex in expression of retrotransposon LINE-1
LEE Sunmin, 月生雅也, SURYANI Budirahardja Yemima, 木下菜月, 谷口慎也, 橋本佳樹, 建石寿枝, 杉本直己, 三好大輔, 川内敬子
日本分子生物学会年会プログラム・要旨集(Web) 45th 2022
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The role of G-quadruplex derived from ribosomal DNA in nucleolar LLPS
取井猛流, 杉本渉, 建石寿枝, 木下菜月, 鶴田充生, 月生雅也, 村嶋貴之, 西方敬人, 杉本直己, 三好大輔, 川内敬子
日本分子生物学会年会プログラム・要旨集(Web) 45th 2022
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“Development of New Materials and New Functions for Biomaterials” Molecularly Targeted Molecules for Non-Canonical Nucleic Acid Structures Stabilized Under Cellular Molecular Crowding Conditions
川内敬子, 橋本佳樹, 杉本渉, 三好大輔
バイオマテリアル(Web) 40 ( 3 ) 2022
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Future perspective of ligands for G-quadruplex nucleic acids as therapeutic agents
木下菜月, 取井猛流, 川内敬子, 三好大輔
Bio Clinica 37 ( 4 ) 2022
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Photodynamic therapy targeting nucleic acid G-quadruplexes
取井猛流, 木下菜月, 浦野諒人, 三好大輔, 川内敬子
日本女性科学者の会学術誌(Web) 22 2022
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Liquid-liquid phase separation of G4-forming cancer-related mRNAs
小路諭, 赤松由御, 鶴田充生, 川内敬子, 三好大輔
日本化学会春季年会講演予稿集(Web) 102nd 2022
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The role of G-quadruplex in expression of retrotransposon LINE-1
LEE Sunmin, 月生雅也, SURYANI Budirahardja Yemima, 木下菜月, 谷口慎也, 橋本佳樹, 建石寿枝, 杉本直己, 三好大輔, 川内敬子
日本分子生物学会年会プログラム・要旨集(Web) 45th 2022
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The anti-cancer effect of deferriferrichrysin produced by Aspergillus oryzae
豊田駿, 芦田侑加子, 赤松実憲, 浦野諒人, 月生雅也, 取井猛流, 戸所健彦, 久田博元, 石田博樹, 西方敬人, 川内敬子
日本薬学会年会要旨集(Web) 142nd 2022
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Inhibitory mechanism of diols for biological LLPS
高宮渚, 鶴田充生, 川内敬子, 三好大輔
日本化学会春季年会講演予稿集(Web) 102nd 2022
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Droplet degradation targeted at secondary structure of nucleic acid
鶴田充生, 取井猛流, 小畠一起, 川内敬子, 建石(狩俣)寿枝, 杉本直己, 三好大輔
日本化学会春季年会講演予稿集(Web) 102nd 2022
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Nanomedicine Using Photosensitive Nanoparticles
西館智尋, 金野智浩, 坂本武琉, 木下菜月, 鳥井猛流, 橋本佳樹, 川内敬子, 三好大輔, 今井陽介, 小林芳男, 山内紀子, 北村成史, 権田幸祐
ナノ学会大会講演予稿集 20th 2022
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A Novel G4 Ligand Screening System with G4-selective Fluorescent Probe
橋本佳樹, 取井猛流, 藤田ひな, 川内敬子, 建石寿枝, 杉本直己, 三好大輔
日本化学会春季年会講演予稿集(Web) 102nd 2022
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The role of G-quadruplex derived from ribosomal DNA in nucleolar LLPS
取井猛流, 杉本渉, 建石寿枝, 木下菜月, 鶴田充生, 月生雅也, 村嶋貴之, 西方敬人, 杉本直己, 三好大輔, 川内敬子
日本分子生物学会年会プログラム・要旨集(Web) 45th 2022
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核酸医薬の配列設計のためのin vitro評価：細胞内物質との弱い相互作用の影響 Invited
中野修一
Bio Clinica 12 ( 36 ) 1426 - 1428 2021.12
Authorship：Lead author, Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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マイクロ波照射による細胞への影響とその応用 Reviewed
平田 桃, 川内 敬子, 西方 敬人, 中西 伸浩, 臼井 健二
日本電磁波エネルギー応用学会論文誌 5 38 - 48 2021.12
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適用範囲の広い皮膚感作性試験法の開発
臼井健二,宮﨑洋,山下邦彦
Medical Science Digest 47 226 - 228 2021.3
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核小体ストレスにより形成するrDNA G-quadruplex集合体とその機能
杉本 渉, 桑本 慎, 建石 寿枝, 岩根 敦子, 高橋 宏隆, 三好 大輔, 川内 敬子
日本薬学会年会要旨集 141年会 29V05 - am10S 2021.3
Publisher：(公社)日本薬学会
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Analysis of liquid-liquid phase separation phenomenon by interaction between rDNA-derived DNA G4 and RGG peptide
月生雅也, 取井猛流, 建石寿枝, 西方敬人, 杉本直己, 三好大輔, 川内敬子
日本分子生物学会年会プログラム・要旨集(Web) 44th 2021
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Molecular mechanism of the G-quadruplex formation for ribosomal DNA
取井猛流, 杉本渉, 建石寿枝, 鶴田充生, 月生雅也, 村嶋貴之, 西方敬人, 杉本直己, 三好大輔, 川内敬子
日本分子生物学会年会プログラム・要旨集(Web) 44th 2021
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核酸医薬の化学 総論 −修飾核酸と機能評価
秋田智香、川上純司
実験医学 39 ( 17 ) 2716 - 2722 2021
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Molecularly targeted photodynamic therapy for cancer mRNAs
杉本渉, 橋本佳樹, 川内敬子, 三好大輔
Bio Clinica 36 ( 5 ) 2021
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Effects of epigenetic and epitranscriptomic modifications of nucleic acids on local structure, global structure, and phase separation
鶴田充生, 取井猛流, 杉本渉, 川内敬子, 三好大輔
月刊細胞 53 ( 14 ) 2021
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Microwave Effects on Cells and Applications to Cancer Therapy
平田桃, 川内敬子, 西方敬人, 西方敬人, 中西伸浩, 中西伸浩, 臼井健二, 臼井健二
日本電磁波エネルギー応用学会論文誌(Web) 5 2021
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Analysis of liquid-liquid phase separation phenomenon by interaction between rDNA-derived DNA G4 and RGG peptide
月生雅也, 取井猛流, 建石寿枝, 西方敬人, 杉本直己, 三好大輔, 川内敬子
日本分子生物学会年会プログラム・要旨集(Web) 44th 2021
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Exploration of sequence selectivity of peptide inducing liquid-liquid phase separation
鶴田充生, 小畠一起, 取井猛流, 川内敬子, 三好大輔
日本化学会春季年会講演予稿集(Web) 101st 2021
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Development of screening system for the discovery of small molecules that regulate liquid-liquid phase separation
高宮渚, 鶴田充生, 橋本佳樹, 川内敬子, 三好大輔
日本化学会春季年会講演予稿集(Web) 101st 2021
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A copolymer containing vitamin E and monosaccharide induced cell death toward breast cancer cells
松田萌美, 北爪琢哉, 遊佐真一, 川内敬子, 大谷亨
日本バイオマテリアル学会大会予稿集(Web) 43rd 2021
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Photosensitizers Based on G-Quadruplex Ligand for Cancer Photodynamic Therapy. International journal
Kawauchi K, Urano R, Kinoshita N, Kuwamoto S, Torii T, Hashimoto Y, Taniguchi S, Tsuruta M, Miyoshi D
Genes 11 1340 2020.11
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：MDPI
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難水溶性物質の皮膚感作性を評価する簡便な試験法の開発 Reviewed
有海 秀人, 浜田 芳男, 臼井 健二, 吉山 友二
臨床薬理 51 ( Suppl. ) S311 - S311 2020.10
Publisher：(一社)日本臨床薬理学会
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How does intracellular environment affect oligonucleotides used for nucleic acid pharmaceuticals? Invited
Shu-ichi Nakano
52 ( 5 ) 42 - 44 2020.5
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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洗浄処理後のすすぎ信憑性評価のための生物学的な安全性評価試験方法の検討 Reviewed
川田原 瑠勇, 藤田 敏, 原田 陽滋, 臼井 健二
医療機器学 90 ( 2 ) 179 - 179 2020.4
Publisher：(一社)日本医療機器学会
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HIV-1プロテアーゼ阻害剤の水溶性プロドラッグ Reviewed
浜田 芳男, 臼井 健二
日本薬学会年会要旨集 140年会 27P - am018 2020.3
Publisher：(公社)日本薬学会
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Liquid-liquid phase separation of short G-quadruplex RNA oligonucleotides and RGG oligo peptides
小畠一起, 杉本渉, 川内敬子, 杉本直己, 杉本直己, 三好大輔
日本化学会春季年会講演予稿集(CD-ROM) 100th 2020
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The mecanism of nucleolus disruption by inhibiting rRNA transcription
杉本渉, 豊田駿, 建石寿枝, 杉本直己, 岩根敦子, 岩根敦子, 岩根敦子, 三好大輔, 川内敬子
日本薬学会年会要旨集(CD-ROM) 140th (Web) 2020
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DMPKの新たな発現機構の解明
取井猛流, 江畑貴弘, 伊藤功彦, 平田宏聡, 杉本渉, 小野寺恵吾, 中嶋亘, 上原郁野, 奥崎大介, 山内翔太, YEMIMA Budirahardja, 西方敬人, 三好大輔, 田中信之, 川内敬子, 川内敬子
日本分子生物学会年会プログラム・要旨集(Web) 43rd 2020
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静水圧における細胞移動の分子機構の解明
谷口慎也, 木下菜月, 取井猛流, 杉本渉, 西方敬人, 平田宏聡, 吉野大輔, 川内敬子
日本分子生物学会年会プログラム・要旨集(Web) 43rd 2020
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Liquid-liquid phase separation of short G-quadruplex RNA oligonucleotides and RGG oligo peptides
小畠一起, 杉本渉, 川内敬子, 杉本直己, 杉本直己, 三好大輔
日本化学会春季年会講演予稿集(CD-ROM) 100th 2020
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The roles of novel target genes of YAP in breast cancer cells
川内敬子, 吉野大輔, 平田宏聡
日本分子生物学会年会プログラム・要旨集(Web) 43rd 2020
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TMPRSS2遺伝子における四重鎖形成配列の同定
木下菜月, 中田実紀, 谷口慎也, 橋本佳樹, 取井猛流, 川内敬子, 三好大輔
日本分子生物学会年会プログラム・要旨集(Web) 43rd 2020
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固液界面での選択的 MOF 形成 Invited
鶴岡孝章, 平尾翔也, 髙嶋洋平, 赤松謙祐
日本接着学会誌 55 301 - 37 2019.8
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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C-SPRAにおける難水溶性感作性物質に対する適用性の検討 Reviewed
池田 英史, 宮崎 洋, 山下 邦彦, 高石 雅之, 濱田 芳男, 臼井 健二
The Journal of Toxicological Sciences 44 ( Suppl. ) S320 - S320 2019.6
Publisher：(一社)日本毒性学会
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分子夾雑環境が制御する核酸分子の機能
中野修一、杉本直己
化学と工業 72 ( 5 ) 401 - 403 2019.5
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：日本化学会
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皮膚の安全性や有用性を考慮する評価法とその治療法の開発 固定化ペプチドを用いた新規感作性物質評価法 Reviewed
浜田 芳男, 臼井 健二
日本薬学会年会要旨集 139年会 ( 1 ) 184 - 184 2019.3
Publisher：(公社)日本薬学会
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皮膚の安全性や有用性を考慮する評価法とその治療法の開発 ペプチドマイクロアレイを化学物質の皮膚への安全性評価に応用する Reviewed
臼井 健二
日本薬学会年会要旨集 139年会 ( 1 ) 183 - 183 2019.3
Publisher：(公社)日本薬学会
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プロテアーゼ処理によるアミロイドβペプチドの部分配列から成るナノ構造体の細胞接着能 Reviewed
横田晋一朗, 眞下泰正, 原田陽滋, 三重正和, 小畠英理, 臼井健二
日本化学会春季年会講演予稿集(CD-ROM) 99th 2019
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化学的表面処理によるポリイミド樹脂のダイレクトメタライゼーション Invited
赤松 謙祐, 髙嶋 洋平, 鶴岡 孝章
表面技術 70 182 - 187 2019
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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イオン交換基を利用した多孔性金属錯体内での機能性物質の固定化と機能開拓 Invited
髙嶋洋平
43 25 - 27 2019
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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第６節 ペプチド固定化樹脂を用いた皮膚感作性試験法の開発 Reviewed
臼井健二, 宮﨑洋, 南野祐槻, 濵田芳男, 山下邦彦
皮膚の安全性・有用性評価法 88 - 95 2018.5
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Peptides for Silica Precipitation: Amino Acid Sequences for Directing Mineralization Reviewed
Makoto Ozaki, Shungo Sakashita, Yoshio Hamada, Kenji Usui
Protein & Peptide Letters 25 15 - 24 2018.4
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Bentham Science Publishers
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細胞核指向型ペプチド-プラスミドDNA複合体の合成と赤色蛍光タンパク質発現の試み Reviewed
片岡駿佑, 臼井健二, 山崎正幸, 富崎欣也
日本化学会春季年会講演予稿集(CD-ROM) 98th 2018
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アミロイド線維のプロテアーゼ分解によるナノ構造体の細胞培養基材への応用展開 Reviewed
横田晋一朗, 眞下泰正, 浜田芳男, 原田陽滋, 三重正和, 小畠英理, 臼井健二
日本化学会春季年会講演予稿集(CD-ROM) 98th 2018
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「ポリイミドフィルムの表面改質と銅薄膜のダイレクトめっき技術」
高嶋洋平、鶴岡孝章、赤松謙祐
MATERIAL STAGE 18 29 - 33 2018
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「多孔性金属錯体を焼いてナノ粒子合成！？ 汎用性の高い還元的アミノ化反応用触媒」
高嶋洋平
化学 73 ( 3 ) 61 - 62 2018
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Formation of beta-(1,3-1,6)-D-glucan-complexed [70]fullerene and its photodynamic activity towards macrophages (vol 15, pg 1990, 2017) International journal
Atsushi Ikeda, Motofusa Akiyama, Kouta Sugikawa, Kazuya Koumoto, Yuta Kagoshima, Jiawei Li, Toshio Suzuki, Takeshi Nagasaki
ORGANIC & BIOMOLECULAR CHEMISTRY 15 ( 45 ) 9734 - 9734 2017.12
Publisher：ROYAL SOC CHEMISTRY
Correction for 'Formation of β-(1,3-1,6)-d-glucan-complexed [70]fullerene and its photodynamic activity towards macrophages' by Atsushi Ikeda et al., Org. Biomol. Chem., 2017, 15, 1990-1997.
DOI： 10.1039/c7ob90179h
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マイクロビーズ固定化ペプチドを用いたバイオ計測デバイスの開発
臼井 健二, 南野 祐槻, 宮﨑 洋, 横田晋一朗, 山下 邦彦, 濵田 芳男
医療・診断をささえるペプチド科学 298 - 305 2017.10
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光還元法による樹脂上への金属皮膜形成
藤原 良輔, 平木 秀典, 杉谷 優衣, 高嶋 洋平, 鶴岡 孝章, 赤松 謙祐
マイクロエレクトロニクスシンポジウム論文集 27 415 - 418 2017.8
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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OPA蛍光法による医療器材の残留タンパク質抽出方法の検討 Reviewed
原田 陽滋, 三軒 隼人, 川田原 瑠勇, 藤田 敏, 臼井 健二
医療機器学 87 ( 2 ) 168 - 168 2017.4
Publisher：(一社)日本医療機器学会
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細胞毒性からみる各種洗浄剤の毒性評価結果 Reviewed
川田原 瑠勇, 三軒 隼人, 藤田 敏, 林 耕司, 原田 陽滋, 臼井 健二
医療機器学 87 ( 2 ) 221 - 221 2017.4
Publisher：(一社)日本医療機器学会
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P3部位に環状構造を有するβ-セクレターゼ阻害剤の設計 Reviewed
浜田 芳男, 木曽 良明, 臼井 健二
日本薬学会年会要旨集 137年会 ( 2 ) 229 - 229 2017.3
Publisher：(公社)日本薬学会
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Cell and Molecular Mechanics in Health and Disease
Kawauchi K, Fujita H, Miyoshi D, Yim EK., Hirata H
BioMed Research International 2017.2
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：hindawi
DOI： 10.1155/2017/2860241
Other Link： https://www.hindawi.com/journals/bmri/2017/2860241/
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プロテアーゼを用いたアミロイド線維の分解による新規ナノ構造体の作製 Reviewed
龍見栄太, 川田原瑠偉, 原田陽滋, 浜田芳男, 臼井健二
日本化学会春季年会講演予稿集(CD-ROM) 97th 2017
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Functions of the tumor suppressors p53 and Rb in actin cytoskeleton remodeling
Ebata T, Hirata H, Kawauchi K
BioMed Research International 2016.12
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Hindawi
DOI： 10.1155/2016/9231057
Other Link： https://www.hindawi.com/journals/bmri/2016/9231057/
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The role of the actin cytoskeleton in p53-mediated tumor suppression
Kawauchi K
The proceeding of WGC-2016 103 2016.11
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：BIT Group Global Ltd.
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A novel mechanism of p53-mediated apoptosis via remodeling of the actin cytoskeleton
Ebata T, Kawauchi K
The proceeding of WGC-2016 381 2016.11
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：BIT Group Global Ltd.
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有機-無機ハイブリッド材料創製を指向した人工ペプチドを用いたミネラリゼーションの制御
臼井健二, 富﨑欣也
化学工業 67 ( 10 ) 721 - 728 2016.10
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：化学工業社
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A NOVEL N-TERMINAL DEGRADATION REACTION OF PEPTIDES VIA GUANIDINE DERIVATIVES
Y. Hamada, K. Usui
JOURNAL OF PEPTIDE SCIENCE 22 S204 - S204 2016.9
Publishing type：Research paper, summary (international conference) Publisher：WILEY
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プロテアーゼによるアミロイド凝集体分解過程の簡便な検出・解析手法の検討 Reviewed
臼井 健二, 柳原 太志, 龍見 栄太, 川田原 瑠勇, 三軒 隼人, 藤田 敏, 原田 陽滋
医療機器学 86 ( 2 ) 146 - 146 2016.4
Publisher：(一社)日本医療機器学会
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ペプチド-核酸複合ナノ構造体を形成する線維化ペプチドと二次構造形成DNAの探索
下岡正幸, 岡田亜梨沙, 柳原太志, 臼井健二
日本化学会春季年会講演予稿集(CD-ROM) 96th 2016
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「樹脂の環境調和型ダイレクトめっき技術」
鶴岡孝章、高嶋洋平、赤松謙祐
プラスチック 67 ( 5 ) 25 - 28 2016
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実験教材の製作
西方敬人，柏崎寿宣，谷田部弘，小林正浩
「新たなものづくり」3Dプリンタ活用最前線 169 - 177 2015.12
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：NTS社
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p53 regulates cytoskeleton remodeling to suppress tumor progression
Araki K, Ebata T, Guo K A, Tobiume K, Wolf JS, Kawauchi K
Cellular and Molecular Life Sciences 72 ( 21 ) 4077 - 4094 2015.11
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Springer
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化学修飾技術を利用した乳酸菌模倣粒子による免疫賦活 Invited
甲元一也、長濱宏治、松井淳
バイオインダストリー 32 ( 9 ) 42 - 47 2015.9
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
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Label and Label-Free Detection Techniques for Protein Microarrays Reviewed International coauthorship
A. Syahir, K. Usui, K.-y. Tomizaki, K. Kajikawa, H. Mihara
Microarrays 4 ( 2 ) 228 - 244 2015.4
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Multidisciplinary Digital Publishing Institute
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光切断リンカーを介した固定化によるアミロイドペプチドの新規モノマー化法及び線維化アッセイ法の構築 Reviewed
疋田晋也, 岡平理湖, 臼井健二
日本化学会講演予稿集 95th ( 3 ) 2015
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アミロイドβペプチドとグアニンリッチDNAを用いた新規ナノ構造体の作製 Reviewed
下岡正幸, 岡田亜梨沙, 柳原太志, 臼井健二
日本化学会講演予稿集 95th ( 3 ) 2015
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「金属ナノ粒子/多孔性有機金属錯体コアシェルナノ構造体の作製」
鶴岡孝章, 大橋卓史, 高嶋洋平, 赤松謙祐
化学工業 66 25 - 28 2015
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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「X線光電子分光」
高嶋洋平、鶴岡孝章、赤松謙祐
めっき技術 28 ( 1 ) 95 - 98 2015
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第46回若手ペプチド夏の勉強会開催 Reviewed
臼井健二
Peptide Newsletter Japan 94 2014.10
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Understanding p53: new insights into tumor suppression
Kawauchi K, Wolf JS.
Expert Review of Anticancer Therapy 14 ( 10 ) 1101 - 1103 2014.10
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Talylar & Francis Group content
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Loss of p53 Enhances NF-kappa B-Dependent Lamellipodia Formation (vol 229, pg 696, 2014)
A. K. Guo, Y. Y. Hou, H. Hirata, S. Yamauchi, A. K. Yip, K-H. Chiam, N. Tanaka, Y. Sawada, K. Kawauchi
JOURNAL OF CELLULAR PHYSIOLOGY 229 ( 10 ) 1569 - 1569 2014.10
Publisher：WILEY-BLACKWELL
DOI： 10.1002/jcp.24651
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A simple "add and measure" FRET-based telomeric tandem repeat sequence detection and telomerase assay method.
K. Kawamura, H. Yaku, D. Miyoshi, T. Murashima
Org. Biomol. Chem. 12 936 - 941 2014
DOI： 10.1039/c3ob42092b
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In vitro assays predictive of telomerase inhibitory effect of G-quadruplex ligands in cell nuclei.
H. Yaku, T. Murashima, D. Miyoshi, N. Sugimoto,
J. Phys. Chem. B 118 2605 - 2614 2014
DOI： 10.1021/jp410669t
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In situ synthesis of metal/polymer nanocomposite thin films on glass substrates by using highly cross-linked polymer matrices with tailorable ion exchange capabilities.
I. Toda, T. Tsuruoka, J. Matsui, T. Murashima, H. Nawafune, K. Akamatsu
RSC Advances, 4 4723 - 4726 2014
DOI： 10.1039/c3ra46166a
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電気化学的手法によるポリイミド系樹脂上への金属薄膜形成プロセス
木村 祐介, 藤原 良輔, 高嶋 洋平, 鶴岡 孝章, 縄舟 秀美, 柳本 博, 赤松 謙祐
マイクロエレクトロニクスシンポジウム論文集 24 223 - 226 2014
Publishing type：Rapid communication, short report, research note, etc. (scientific journal)
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代謝産物アナログによる酵素活性化・安定化
高木琴味、甲元一也
月刊バイオインダストリー 30 ( 8 ) 43 - 50 2013.8
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：CMC出版
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A PNA Peptide for the Control of Site-Specific Silica Precipitation on DNA Reviewed
USUI Kenji, NAGAI Kazuma, NISHIYAMA Hiroto, TSURUOKA Takaaki, FUJII Satoshi
2012 375 - 376 2013.3
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Construction of Artificial Peptides to Control DNA G-quadruplex Structures Depending on Protease Activity for Regulation of Protein Expression Reviewed
OKADA Arisa, USUI Kenji, TANIGUCHI Mai, KOBAYASHI Keita, SUGIMOTO Naoki
2012 285 - 286 2013.3
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金属ナノ粒子分散層を利用した樹脂/金属間接合
鶴岡、縄舟、赤松
プラスチックスエージ Ｍａｒ 55 - 60 2013
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：プラスチックスエージ
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透明ポリイミドフィルム上へのピール強度の優れた銅めっき皮膜形成
池田、小林、藤原、赤松、縄舟
工業材料 61 ( 5 ) 47 - 50 2013
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：日刊工業新聞
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電気化学的手法によるポリイミド樹脂表面への金属パターン形成
赤松、鶴岡、縄舟
工業材料 61 ( 5 ) 39 - 42 2013
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：日刊工業新聞
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マテリアル・イノベーションに対応する高度めっき技術
縄舟、赤松、鶴岡
工業材料 61 ( 5 ) 18 - 22 2013
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：日刊工業新聞
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A highly sensitive telomerase activity assay that eliminates false-negative results caused by PCR inhibitors
H. Yaku, T. Murashima, D. Miyoshi, N. Sugimoto
Molecules 18 11751 - 11767 2013
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Study on effects of molecular crowding on G-quadruplex-ligand binding and ligand-mediated telomerase inhibition
H. Yaku, T. Murashima, H. Tateishi-Karimata, S. _I. Nakano, D. Miyoshi, N. Sugimoto
Methods 64 19 - 27 2013
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Multiple and cooperative binding of fluorescence light-up probe thioflavin t with human telomere DNA G-quadruplex
V. Gabelica, R. Maeda, T. Fujimoto, H. Yaku, T. Murashima, N. Sugimoto, D. Miyoshi
Biochemistry 52 5620 - 5628 2013
DOI： 10.1021/bi4006072
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In situ template synthesis of one-dimensional gold nanoparticle arrays in organic nanowires
T. Matsushita, Y. Fukumoto, T. Kawakami, T. Tsuruoka, T. Murashima, T. Yanagishita, H. Masuda, H. Nawafune, K. Akamatsu
RSC Advances 3 16243 - 16246 2013
DOI： 10.1039/c3ra42727g
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金属ナノ粒子分散層を利用した樹脂/金属間接合
縄舟、赤松、鶴岡
プラスチックスエージ Mar 55 - 60 2013
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：プラスチックスエージ
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電気化学的手法によるポリイミド樹脂表面への金属パターン形成
赤松、鶴岡、縄舟
工業材料 61 ( 5 ) 39 - 42 2013
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：日刊工業新聞
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マテリアル・イノベーションに対応する高度めっき技術
縄舟、赤松、鶴岡
工業材料 61 ( 5 ) 18 - 22 2013
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：日刊工業新聞
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Designed peptide with peptide nucleic acids for addressable precipitation of silica on DNA
Kenji Usui, Kazuma Nagai, Hiroto Nishiyama, Tamaki Endoh, Takaaki Tsuruoka, Satoshi Fujii
PROTEIN SCIENCE 21 122 - 123 2012.8
Publishing type：Research paper, summary (international conference) Publisher：WILEY-BLACKWELL
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Designed peptides with nucleic acid bases for control of DNA G-quadruplex structures depending on protease activity
Arisa Okada, Mai Taniguchi, Keita Kobayashi, Kenji Usui, Naoki Sugimoto
PROTEIN SCIENCE 21 74 - 74 2012.8
Publishing type：Research paper, summary (international conference) Publisher：WILEY-BLACKWELL
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Designed peptide with peptide nucleic acids for addressable precipitation of silica on DNA
Kenji Usui, Kazuma Nagai, Hiroto Nishiyama, Tamaki Endoh, Takaaki Tsuruoka, Satoshi Fujii
PROTEIN SCIENCE 21 122 - 123 2012.8
Publisher：WILEY-BLACKWELL
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“オミクス”研究に向けた設計ペプチドアレイの構築 Reviewed
臼井健二、富崎欣也、三原久和
分析化学 61 523 - 534 2012.6
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：日本分析化学会
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電気化学リソグラフィーによる樹脂表面への金属パターン形成
赤松、福本、鶴岡、縄舟、柳本
表面技術 63 95 - 99 2012
Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：表面技術協会
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Colorimetric Detection of Perfluorooctanoic Acid (PFOA) Utilizing Polystyrene-Modified Gold Nanoparticles
M. Takayose, K. Akamatsu, H. Nawafune, T. Murashima, J. Matsui
Anal. Lett., 45 2856 - 2864 2012
-
Specific binding of anionic porphyrin and phthalocyanine to the G-quadruplex with a variety of in Vitro and in Vivo applications
H. Yaku, T. Murashima, D. Miyoshi, N. Sugimoto
Molecules 17 10586 - 10613 2012
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Phthalocyanines: A new class of G-quadruplex-ligands with many potential applications
H. Yaku, T. Fujimoto, T. Murashima, D. Miyoshi, N. Sugimoto
Chem. Commun., 48 6203 - 6216 2012
DOI： 10.1039/c2cc31037f
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Mechanistic study on microstructural tuning of metal nanoparticle/polymer composite thin layers: Hydrogenation and decomposition of polyimide matrices catalyzed by embedded nickel nanoparticles
K. Akamatsu, M. Fujii, T. Tsuruoka, S._I. Nakano, T. Murashima, H. Nawafune
J. Phys. Chem. C, 116 17947 - 17954 2012
DOI： 10.1021/jp304702b
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電気化学リソグラフィーによる樹脂表面への金属パターン形成
赤松、福本、鶴岡、縄舟、柳本
表面技術 63 95 - 99 2012
Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：表面技術協会
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ポリイミド樹脂/金属間におけるナノ構造界面の構築と樹脂上への金属パターン形成
福本ユリナ、谷山智紀、鶴岡孝章、縄舟秀美、赤松謙祐
エレクトロニクス実装学会 15 534 - 540 2012
Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：エレクトロニクス実装学会
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Cellular zwitterionic metabolite analogs simultaneously enhance reaction rate, thermostability, salt tolerance, and substrate specificity of alpha-glucosidase International journal
Eisuke Deguchi, Kazuya Koumoto
BIOORGANIC & MEDICINAL CHEMISTRY 19 ( 10 ) 3128 - 3134 2011.5
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
We investigated the structural effects of metabolite analogs derived from a naturally-occurring zwitterionic metabolite, glycine betaine, on the activity of several hydrolases. The initial velocities of the hydrolases were enhanced by the addition of the solutes into the buffer solution. Based on a detailed study using alpha-glucosidases, the acceleration efficiency of the enzymatic activity was strongly induced by solutes possessing bulky and aliphatic ammonium cations, indicating that enhancement of activity by the solutes depended on their chemical structures. Kinetic analysis revealed that the acceleration of the hydrolysis reaction was related to both the decrement of K-m and increment of V-max values. Furthermore, the addition of the metabolite analogs enhanced not only the rate constant but also the thermostability, salt tolerance, and substrate specificity of alpha-glucosidase simultaneously through the reduction of conformational perturbation of the enzyme. (C) 2011 Elsevier Ltd. All rights reserved.
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踏み出そう！新たなめっき技術へ
縄舟秀美、赤松謙祐、鶴岡孝章
表面技術 62 584 - 588 2011
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：表面技術協会
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比抵抗の大きな基材への直接銅電析と析出皮膜の物性
有村、鶴岡、赤松、縄舟
表面技術 62 448 - 450 2011
Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：表面技術協会
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金属・磁性ナノ粒子を機能性ユニットとしたナノ構造材料の創製
鶴岡孝章、縄舟秀美、赤松謙祐
鉱山 64 ( 1 ) 42 - 48 2011
Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：金属鉱山会
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金属ナノ粒子と高分子の複合材料の精密設計
赤松謙祐
高分子 60 ( 6 ) 397 - 398 2011
Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：高分子学会
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A NOVEL NITRIC OXIDE SENSOR USING FLUORESCENT PEPTIDES ATTACHED TO IRON COMPLEXES
H. Miyazaki, K. Usui, S. Fujii
BIOPOLYMERS 96 ( 4 ) 510 - 510 2011
Publishing type：Research paper, summary (international conference) Publisher：WILEY-BLACKWELL
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微細配線技術へのめっき技術の適用
縄舟、赤松、鶴岡
電気化学 79 172 - 178 2011
Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：電気化学会
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踏み出そう！新たなめっき技術へ
縄舟、赤松、鶴岡
表面技術 62 584 - 588 2011
Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：表面技術協会
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比抵抗の大きな基材への直接銅電析と析出皮膜の物性
有村、鶴岡、赤松、縄舟
表面技術 62 448 - 450 2011
Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：表面技術協会
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金属・磁性ナノ粒子を機能性ユニットとしたナノ構造材料の創製
鶴岡、縄舟、赤松
鉱山 ( 689 ) 42 - 48 2011
Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：金属鉱山会
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化学ツールとしての分子クラウディング
中野修一・杉本直己
化学フロンティア22 生命現象を理解する分子ツール最前線〜イメージングから生体機能解析まで〜 145 - 153 2010.9
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：化学同人
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Fluorescent and luminescent fusion proteins for detection of amyloid beta peptide localization and aggregation
K. Usui, M. Mie, T. Andou, N. Sugimoto, H. Mihara, E. Kobatake
JOURNAL OF PEPTIDE SCIENCE 16 175 - 175 2010.9
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS LTD
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第１０章 デザインペプチドを用いたプロテインアレイと細胞アレイ
臼井健二, 富崎欣也, 三原久和
化学フロンティア２２ 生命現象を理解する分子ツール、(化学同人) 79 - 87 2010.9
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Protein-protein interactions and selection: array-based techniques for screening disease-associated biomarkers in predictive/early diagnosis Reviewed
K.-Y. Tomizaki, K. Usui, H. Mihara
FEBS Journal 277 1996 - 2005 2010.5
Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：the Federation of European Biochemical Societies
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生体シグナル解析用の機能性核酸センサーの定量的デザイン
中野修一・杉本直己
シングルセル解析の最前線 9 - 16 2010.3
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：シーエムシー出版
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第一節 DNAチップの基礎１ ハイブリダイゼーション
杉本直己・中野修一
バイオチップ実用化ハンドブック 13 - 19 2010.3
Publisher：エヌティーエス
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糖分子を認識する人工レセプター：ボロン酸
甲元一也、新海征治
月刊バイオインダストリー 27 ( 2 ) 18 - 23 2010.2
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：CMC出版
水中で糖のもつ水酸基と可逆的なエステル結合を形成するボロン酸基を有する糖レセプターの設計、合成に関する動向を纏めた。
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糖分子を認識する人工レセプター：ボロン酸
甲元一也, 新海征治
バイオインダストリー 27 ( 2 ) 66 - 72 2010
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金属ナノ粒子複合体の合成と構造制御
赤松謙祐
コロイドアンドインターフェースコミュニケーションNews Letter 35 2010
Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：a
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Proteins: Array-Based Techniques Reviewed
K.-Y. Tomizaki, K. Usui and H. Mihara
Wiley Encyclopedia of Chemical Biology 4 144 - 158 2009.1
Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Wiley
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Reversible stability switching of a hairpin DNA via a photo-responsive linker unit International journal
Li Wu, Kazuya Koumoto, Naoki Sugimoto
CHEMICAL COMMUNICATIONS ( 14 ) 1915 - 1917 2009
Publisher：ROYAL SOC CHEMISTRY
We have designed and synthesized a novel photo-responsive linker unit, 4,4'-bis(hydroxymethyl)-azobenzene, which was introduced to the loop oligonucleotide in a DNA hairpin and was able to reversibly control the stability of the whole hairpin structure via UV or visible light irradiation.
DOI： 10.1039/b819643e
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Molecularly imprinted nanocomposites for highly sensitive SPR detection of a non-aqueous atrazine sample
Jun Matsui, Megumi Takayose, Kensuke Akamatsu, Hidemi Nawafune, Katsuyuki Tamaki, Naoki Sugimoto
ANALYST 134 ( 1 ) 80 - 86 2009
Publisher：ROYAL SOC CHEMISTRY
A surface plasmon resonance (SPR) sensor highly sensitive and selective for the herbicide atrazine was composed by immobilizing atrazine-imprinted polymer with gold nanoparticles on a gold thin film as a sensor chip. In the detection, the atrazine-imprinted polymer was expected to work as synthetic receptor for selectively capturing atrazine in organic solvent, and the gold nanoparticles were expected to exhibit a coupling effect with the gold thin film to enhance the local electromagnetic field between the nanoparticles and the gold film, making the sensor chip highly sensitive for changes in microenvironmental polarity. Thus, a combination of the atrazine-imprinted polymer and gold nanoparticles enabled us to compose an SPR sensor demonstrating the detection of 5 pM atrazine in acetonitrile.
DOI： 10.1039/b803350a
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界面微細構造制御による金属／ポリイミド樹脂間の接合
池田慎吾、赤松謙祐、縄舟秀美
日本接着学会誌 45 ( 2 ) 58 - 62 2009
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：日本接着学会
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金属ナノ粒子/ポリマーフォトニック結晶の作製
鶴岡孝章、熊崎祥太、縄舟秀美、赤松謙祐
ケミカルエンジニヤリング 54 49 2009
Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：化学工業社
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強磁性金属ナノコンポジットを用いた左手系メタマテリアル
冨田知志、赤松謙祐、八代晴彦、三俣千春
応用物理 6 523 2009
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：応用物理学会
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Enhancement of photoluminescence from silicon nanocrystals by metal nanostructures made by nanosphere lithography
Y. Mochizuki, M. Fujii, S. Hayashi, T. Tsuruoka, K. Akamatsu
J. Appl. Phys. 106 ( 1 ) 013517 2009
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Site-selective direct nickel metallization on precursor polyimide using microcontact printing
Y. Matsumura, Y. Enomoto, K. Akamatsu, H. Nawafune
J. Surf, Finish, Soc. Jpn. 60 ( 1 ) 61 - 62 2009
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細胞内外でのアミロイドβペプチドの局在と構造変化を検出できる発光・蛍光タンパク質の構築 Reviewed
臼井健二, 三重正和, 杉本直己, 三原久和, 小畠英理
日本化学会バイオテクノロジー部会シンポジウム講演要旨集 12th 2009
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種々の細胞系を用いた新規αヘリックスペプチドの細胞導入活性スクリーニング Reviewed
菊池卓哉, 臼井健二, 高橋剛, 三原久和
日本化学会バイオテクノロジー部会シンポジウム講演要旨集 12th 2009
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金属ナノ粒子／ポリマー複合体フォトニック結晶の作製
鶴岡孝章、熊崎祥太、縄舟秀美、赤松謙祐
ケミカルエンジニヤリング 54 941 - 946 2009
Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：化学工業社
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DNAはなぜ二重らせん構造になるのか？ —分子間相互作用と分子環境効果を考えるー
杉本直己・中野修一
現代化学 450 ( 9 ) 25 - 30 2008.9
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：東京化学同人
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Hydration is an important factor to regulate thermodynamic stability of a DNA duplex under molecular crowding conditions
Kazuya Koumoto, Hirofumi Ochiai, Naoki Sugimoto
CHEMISTRY LETTERS 37 ( 8 ) 864 - 865 2008.8
Publisher：CHEMICAL SOC JAPAN
By using a DNA duplex. (5'-GTTACTATATGA-3'/5'-TCATATAGTAAC-3') under molecular crowding, conditions, it was revealed that hydration plays a significant role in the stability of the duplex, in contrast to viscosity and dielectric constant which have little effect.
DOI： 10.1246/cl.2008.864
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NanoBioNow(10)周辺の分子環境に応答するDNAナノスイッチの構築
狩俣寿枝, 三好大輔, 藤本健史, 甲元一也, WANG Zhong‐Ming, 杉本直己
日本化学会講演予稿集 88th ( 2 ) 823 2008.3
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Structural effect of synthetic zwitterionic cosolutes on the stability of DNA duplexes
Kazuya KouMoto, Hirofumi Ochiai, Naoki Sugimoto
TETRAHEDRON 64 ( 1 ) 168 - 174 2008.1
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
The molecular design of useful cosolutes for polymerase chain reaction (PCR), which is one of the most important techniques in molecular biology, plays a significant role in amplification of highly stable genome sequences because during PCR, strand dissociation sometimes fails due to high melting temperature. Here, we designed and synthesized eight new zwitterionic cosolutes derived from glycine betaine, a destabilizing reagent for GC-rich DNA duplexes, and systematically compared their ability to destabilize DNA duplexes and to amplify genome DNA by PCR. We found that introduction of n-butyl groups rather than methyl groups into the ammonium group reduced the melting temperature of DNA duplexes 11-fold more than what was observed for the scaffold cosolute, glycine betaine, and furthermore, the cosolute can amplify the stable genome sequence by PCR. (c) 2007 Elsevier Ltd. All rights reserved.
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Enhanced amplification of polymerase chain reaction by addition of cosolutes derived from a cellular compatible solute. International journal
Kazuya Koumoto, Hirofumi Ochiai, Naoki Sugimoto
Nucleic acids symposium series (2004) 52 ( 52 ) 257 - 8 2008
We designed and synthesized artificial compatible solutes which can not only decrease the melting temperature of DNA duplexes dependent of their chemical structures but also improve the amplification of highly stable genome DNA sequence.
DOI： 10.1093/nass/nrn130
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Preparation and Characterization of Au Nanoparticle/Polymer Composite Photonic Crystals
松井 淳
Trans. MRS-Japan 33 717 - 720 2008
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Control of the optical properties of CdTe nanocrystals by selective exchange of Te with thiolate: effect of organic ligands on the formation of core-shell structures
T. Tsuruoka, R. Takahashi, K. Akamatsu, and H. Nawafune
Phys. Chem. Chem. Phys. 10 ( 16 ) 2221 - 2226 2008
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Direct metallization of nickel on polymeric template patterns for fabrication of copper circuits on glass substrates
Y. Matsumura, Y. Enomoto, M. Sugiyama, K. Akamatsu, H. Nawafune
J. Mater. Chem. 18 ( 42 ) 5078 - 5082 2008
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Nanocomposite polymeric microspheres containing Ni nanoparticles with controlled microstructures
K. Akamatsu, S. Adachi, T. Tsuruoka, S. Ikeda, S. Tomita, and H. Nawafune
Chem. Mater. 20 ( 9 ) 3042 - 3047 2008
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Immobilization and assembly of mixed monolayer-protected Au nanoparticles on glass substrate via covalent bond interconnection
T. Tsuruoka, A. Fujimoto, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn. 33 157 - 160 2008
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Highly luminescent mono- and multilayers of immobilized CdTe nanocrystals: controlling optical properties through post chemical surface modification
T. Tsuruoka, R. Takahashi, T. Nakamura, M. Fujii, K. Akamatsu, and H. Nawafune
Chem. Commun. ( 14 ) 1641 - 1643 2008
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Formation of metal thin film nanocomposite layer from precursor polyimide containing metal complex
M. Matsumura, Y. Enomoto, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn. 33 1333 - 1336 2008
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Fabrication of conductive thin films on precursor polyimide via chemical reduction of nickel (II) complex
Y. Matsumura, Y. Enomoto, T. Jyo, K. Akamatsu, H. Nawafune
J. Surf, Finish, Soc. Jpn. 59 ( 8 ) 562 - 564 2008
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Cationic Porphyrin Induced a Telomeric DNA to G-Quadruplex Form in Water.
Bioinorg. Chem. Appl., 2008
DOI： 10.1155/2008/294756
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分子クラウディング環境下においてDNA二重鎖の安定性に及ぼす水和の影響
甲元一也, 落合洋文, 狩俣寿枝, 中野修一, 杉本直己
高分子学会予稿集(CD-ROM) 56 ( 2 Disk1 ) 2X15 2007.9
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Delivery of antisense oligonucleotides to nuclear telomere RNA by use of a complex between polysaccharide and polynucleotide
Jusaku Minari, Takanori Kubo, Hideki Ohba, Naohiko Shimada, Yoich Takeda, Ryouji Karinaga, Takahisa Anada, Kazuya Koumoto, Takeshi Kawazu, Takeshi Nagasaki, Seiji Shinkai, Kazuo Sakurai
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 80 ( 6 ) 1091 - 1098 2007.6
Publisher：CHEMICAL SOC JAPAN
Telomerase, which is highly activated in neoplastic cells, can be a target for antisense therapy, and for that purpose, antisense oligonucleotides (AS ODNs) have to be effectively delivered into cellular nucleus where the target telomerase is present. The present work shows a new strategy to deliver AS ODNs to nucleus by use of a novel complex made from a natural polysaccharide schizophyllan (SPG) and AS ODNs. Nuclear transport is strictly regulated by the nuclear pore size and the related proteins. If the molecular weight of SPG is decreased, the SPG/AS ODN complex should be easily transported, although the stability of the complex decreases with a decrease in the molecular weight. We optimized the molecular weight of SPG to be 25 K. Furthermore, we attached importin-beta (a nuclear transport protein) to the side chain of SPG by use of a streptavidin-biotin interaction. When this complex was added to Jurkat cells, the telomerase activity was more suppressed than the naked dose, indicating that the importin-P in the complex induced the nuclear transport of the complexed AS ODN and the AS ODN inhibited the telomerase. The present work shows a new methodology for nuclear anti-sense therapy that should be important in future anti-cancer therapies.
DOI： 10.1246/bcsj.80.1091
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Artificial G-wire switch with 2,2 '-bipyridine units responsive to divalent metal ions International journal
Daisuke Miyoshi, Hisae Karimata, Zhong-Ming Wang, Kazuya Koumoto, Naoki Sugimoto
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 129 ( 18 ) 5919 - 5925 2007.5
Publisher：AMER CHEMICAL SOC
Development of a guanine nanowire (G-wire) that is controllable and can be switched by external signals is important for the creation of molecular electronic technologies. Here, we constructed a G-wire in which the thymines of the main chain of d(G(4)T(4)G(4)) were replaced with 2,2'-bipyridine units, which have two aromatic rings that rotate arbitrarily upon coordination with metal ions. Circular dichroism of the DNA oligonucleotides with or without the 2,2'-bipyridine unit showed that divalent metal ions induce the bipyridine-containing oligonucleotide to switch from an antiparallel to a parallel G-quadruplex. Native polyacrylamide gel electrophoresis showed that the parallel-stranded G-quadruplex DNA had a high-order structure. Circular dichroism and native gel electrophoresis analyses suggested that adding Na(2)EDTA causes a reverse structural transition from a parallel-stranded high-order structure to an antiparallel G-quadruplex. Moreover, atomic force microscopy showed a long nanowire (similar to 200 nm) in the presence of Ni2+ but no significant image in the absence of Ni2+ or in the presence of both Ni2+ and Na(2)EDTA. These observations revealed that the parallel-stranded high-order structure is a G-wire containing numerous DNA oligonucleotide strands bound together via divalent metal ion-2,2'-bipyridine complexes. Finally, we found that alternating addition of Ni2+ and Na(2)EDTA can cycle the G-wire between the high-order and disorganized structures, with an average cycling efficiency of 0.95 (i.e., 5% loss per cycle). These results demonstrate that a DNA oligonucleotide incorporating the 2,2'-bipyridine unit acts as a G-wire switch that can be controlled by chemical input signals, namely, divalent metal ions.
DOI： 10.1021/ja068707u
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細胞膜を通るための必要最低限の疎水性とは？ペプチドを用いた検証
川上純司
高分子 56 ( 3 ) 153 2007.3
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：高分子学会
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Molecular imprinting under molecular crowding conditions: An aid to the synthesis of a high-capacity polymeric sorbent for triazine herbicides
Jun Matsui, Shou Goji, Takashi Murashima, Daisuke Miyoshi, Satoshi Komai, Aiko Shigeyasu, Takuho Kushida, Toshifumi Miyazawa, Takashi Yamada, Katsuyuki Tamaki, Naoki Sugimoto
ANALYTICAL CHEMISTRY 79 ( 4 ) 1749 - 1757 2007.2
Publisher：AMER CHEMICAL SOC
Molecular crowding, an important feature of the molecular environments in biological cells, was applied to the synthesis of antibody-mimic polymers selective for a group of biologically active compounds, the triazine herbicides. Synthesis of these polymers was conducted using molecular imprinting under molecular crowding conditions, whereby atrazine (a template molecule) was complexed with methacrylic acid (a functional monomer) in the presence of a macromolecular crowding agent (either poly(methyl methacrylate) (PMMA) or polystyrene (PS)) followed by cross-linking with ethylene glycol dimethacrylate. After removal of atrazine from the polymer matrix, the retention properties and selectivity of the resultant polymers were assessed by chromatographic tests. The addition of a crowding-inducing agent resulted in polymers with superior retention properties and excellent selectivity for triazine herbicides, as compared to polymers prepared without addition of a crowding-inducing agent. An imprinted polymer prepared in the presence of PS as a crowding agent exhibited a retention factor for atrazine an order of magnitude larger than that of an imprinted polymer prepared in the absence of a crowding agent. NMR results suggest that the crowding agent is capable of promoting hydrogen bond formation between atrazine and methacrylic acid, which could account for the effect of crowding on molecular imprinting.
DOI： 10.1021/ac060441m
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Conformational switch of a functional nanowire based on the DNA G-quadruplex. International journal
Hisae Karimata, Daisuke Miyoshi, Takeshi Fujimoto, Kazuya Koumoto, Zhong-Ming Wang, Naoki Sugimoto
Nucleic acids symposium series (2004) 51 ( 51 ) 251 - 2 2007
A G-wire composing of multiple G-rich sequences in parallel orientations via G-quartet formations is promising as a structurally well-defined nanowire. Here, in order to control the G-wire by the metal ion, we incorporated 2,2'-bipyridine unit, which has two aromatic rings rotating arbitrarily by coordination with metal ions, into the main chain of d(G4T4G4) instead of thymine (G1). Circular dichroism (CD) spectra showed that G1 formed an antiparallel G-quadruplex. Interestingly, Ni(2+) induces G1 to switch from an antiparallel G-quadruplex to a parallel. On the other hand, the parallel G-quadruplex reversed the structural transition to an antiparallel G-quadruplex by the addition of Na2EDTA. Moreover, the study of the atomic force microscopy (AFM) showed that G1 formed a long nanowire ( approximately 200 nm) in the presence of Ni(2+), whereas no significant image was observed in the absence of Ni(2+) or in the presence of both Ni(2+) and Na2EDTA. From these results, it can be concluded that Ni(2+) induces a structural transition of G1 from an antiparallel G-quadruplex to a G-wire and that metal ion chelator induces the reverse structural transition from the G-wire to the antiparallel G-quadruplex. The G-wire responsive to metal ions would be useful for development of functional nanomaterials.
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銅ナノ粒子分散複合体のパターニングと樹脂・金属間接合
池田慎吾、赤松謙祐、縄舟秀美
表面技術 58 756 - 751 2007
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：表面技術協会
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インクジェット法を利用するポリイミド樹脂表面への銅微細配線
赤松謙祐、池田慎吾、縄舟秀美
マテリアルステージ ( 6 ) 15 - 17 2007
Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：a
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Atomic beam-Induced fluorination of polyimide and its application to site-selective Cu metallization
M. Tagawa, K. Maeda, T. Kajita, K. Yokota, K. Akamatsu, H. Nawafune
Langmuir 23 ( 23 ) 11351 - 11354 2007
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Controlled magnetic properties of Ni nanoparticles embedded in polyimide films
S. Tomita, P. E. Jonsson, K. Akamatsu, H. Nawafune, H. Takayama
Phys. Rev. B 76 ( 17 ) 174432 2007
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Copper/polyimide heterojunction: controlling interfacial structures through an additive-based, all-wet chemical process using ion-doped precursors
S. Ikeda, H. Yanagimoto, K. Akamatsu, H. Nawafune
Adv. Funct. Mater. 17 ( 6 ) 889 - 897 2007
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ポリエチレングリコール誘導体の予備吸着を利用する銅電析プロセスによるULSI微細配線の形成
尾山祐斗、有村英俊、赤松謙祐、縄舟秀美
表面技術 58 ( 7 ) 425 - 429 2007
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Photosensitization of oxygen molecules by surface-modified hydrophilic porus Si
M. Fujii, N. Nishimura, H. Fumon, S. Hayashi, K. Akamatsu, T. Tsuruoka, M. Shimada, H. Katayama, D. Kovalev, B. Goller
Euro. Phys. J. D 43 193 - 196 2007
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One-step synthesis of polypyrrole-coated silver nanocomposite particles and their application as a coloured particulate emulsifier
S. Fujii, A. Aichi, K. Akamatsu, H. Nawafune and Y. Nakamura
J. Mater. Chem. 17 ( 36 ) 3777 - 3779 2007
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Synthesis, structure and thermal stability of fully hydrophobic porphyrin-DNA conjugates.
Tetrahedron Lett. 48 ( 48 ) 8514 - 8517 2007
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Secondary alcohols act as better nucleophiles than primary alcohols in the lipase-catalyzed regioselective deacylation of dihydroxybenzenes acylated at both phenolic hydroxyls.
Tetrahedron Lett. 48 ( 47 ) 8334 - 8337 2007
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ペプチドライブラリを用いるタンパク質相互作用検出チップ Reviewed
富崎欣也・臼井健二・三原久和
Electrochemistry 75 ( 12 ) 981 - 985 2007
Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：電気化学会
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細胞の分子クラウディング効果
杉本直己・中野修一
現代化学 428 ( 11 ) 53 - 58 2006.11
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：東京化学同人
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DNAによる三次元ナノ構造体形成法の進展
川上純司
高分子 55 ( 5 ) 362 2006.5
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：高分子学会
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分子クラウディング環境は生命分子の物性と機能をいかに変えるか
中野修一・杉本直己
生物物理 46 ( 5 ) 251 - 256 2006.5
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：日本生物物理学会
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デザイナブル核酸修飾
杉本直己・中野修一・大道達雄
先端生物医学研究・医療のため遺伝子導入テクノロジー「ウイルスを用いない遺伝子導入法の材料、技術、方法論の新たな展開」 245 - 251 2006.4
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：メディカルドゥ
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CpG DNA/zymosan complex to enhance cytokine secretion owing to the cocktail effect
T Anada, N Okada, J Minari, R Karinaga, M Mizu, K Koumoto, S Shinkai, K Sakurai
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 16 ( 5 ) 1301 - 1304 2006.3
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
Zymosan, classified among beta-(1-->3)-D-glucans, is produced from the cell wall of yeast and well known to induce proinflammatory cytokines when ingested by immune cells. We found that zymosan forms a complex with immunostimulatory CpG DNA, where both zymosan and CpG DNA can induce cytokine secretion according to the different mechanisms (i.e., recognized by different receptors). The complex activated macrophages and induced cytokine secretion, more efficiently than separate administration of zymosan or CpG DNA. Microscopic observation showed that this increment of the cytokine secretion can be explained by the fact that zymosan and zymosan/CpG DNA complex are up-taken more than naked CpG DNA. Additionally, existence of two different immunostimulants in the same cells may enhance the immunoresponse. This report presents a new strategy to construct a delivering vehicle for CpG DNA and to enhance its activity with the 'cocktail effect' of the two immunostimulants. (C) 2005 Elsevier Ltd. All rights reserved.
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Galactose-PEG dual conjugation of beta-(1 -> 3)-D-glucan schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake
R Karinaga, T Anada, J Minari, M Mizu, K Koumoto, J Fukuda, K Nakazawa, T Hasegawa, M Numata, S Shinkai, K Sakurai
BIOMATERIALS 27 ( 8 ) 1626 - 1635 2006.3
Publisher：ELSEVIER SCI LTD
Antisense oligonucleotides (AS ODNs) are applied to silence a particular gene, and this approach is one of the potential gene therapies. However, naked oligonucleotides are easy to be degraded or absorbed in biological condition. Therefore, we need a carrier to deliver AS ODNs. This paper presents galactose moieties that were conjugated to the side chain of SPG to enhance cellular ingestion through endocytosis mediated by asialoglycoprotein receptor specifically located on parenchymal liver cells. We introduced galactose with two types of chemical bonds; amide and amine, and the amine connection showed lower ingestion and more toxicity than the amide one. Since PEG was known to induce endocytosis escape, we combined PEG and galactose aiming to provide both cellular up-take and subsequent endocytosis escape. We designed lactose or galactose moieties to attach to the end of the PEG chain that connects to the SPG side chain. When the PEG had the molecular weight of 5000-6000, the antisense effect reached the maximum. We believe that this new type of galactose and PEG dual conjugation broaden the horizon in antisense delivery. (c) 2005 Elsevier Ltd. All rights reserved.
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Molecularly-imprinted polymeric logic gates selective for predetermined chemical input species
Jun Matsui, Takuji Sodeyama, Katsuyuki Tamaki, Naoki Sugimoto
CHEMICAL COMMUNICATIONS ( 30 ) 3217 - 3219 2006
Publisher：ROYAL SOC CHEMISTRY
Selective AND and OR logic gates were fabricated by molecular imprinting with simultaneous use of two kinds of template species, with the resultant binding behaviour (AND or OR) of the imprinted polymers governed by intermolecular interactions between the two template species.
DOI： 10.1039/b604354b
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半導体ナノ粒子の合成と発光特性制御
赤松謙祐、鶴岡孝章、縄舟秀美
科学と工業 80 315 - 321 2006
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：大阪工研協会
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A DNA duplex with extremely enhanced thermal stability based on controlled immobilization on gold nanoparticles
K. Akamatsu, M. Kimura, Y. Shibata, S. Nakano, D. Miyoshi, H. Nawafune, N. Sugimoto
Nano Lett. 6 ( 3 ) 491 - 495 2006
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Fabrication of fluorescent multilayers consisting of CdTe nanocrystals
T. Tsuruoka, R. Takahashi, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn. 31 425 - 428 2006
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Site-selective integration of monolayer-protected inorganic nanoparticles onto surface monolayer templates by solvent-induced lift-off process
K. Akamatsu, S. Samitsu, T. Tsuruoka, J. Hasegawa, H. Nawafune
Small 2 1130 - 1133 2006
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金属イオンレドックス系無電解めっき浴の安定性
赤松謙祐、増田昭仁、尾山祐斗、縄舟秀美
表面技術 57 ( 11 ) 799 - 801 2006
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A peptide array with a functional linker toward a cell assay chip format
T. Kakiyama, K. Usui, K. Tomizaki, H. Mihara
JOURNAL OF PEPTIDE SCIENCE 12 236 - 236 2006
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS LTD
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Detection of Interactions Between the Peptides Containing Nucleobase Amino Acids and Proteins using Anomalous Reflection of Gold Reviewed
渡辺晋也, 臼井健二, 富崎欣也, 梶川浩太郎, 三原久和
日本化学会講演予稿集 86th ( 2 ) 2006
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Construction of a screening system for peptide ligands controlling calcineurin activity using designed α-helical peptide arrays Reviewed
臼井健二, 富崎欣也, 三原久和
日本化学会講演予稿集 86th ( 2 ) 2006
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設計ペプチドを用いるアレイフォーマット開発とタンパク機能解析 Reviewed
臼井健二, 富崎欣也, 渡辺晋也, 三原久和, 梶川浩太郎
高分子学会予稿集(CD-ROM) 55 ( 2 Disk1 ) 2006
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半導体ナノ粒子の合成と発光特性制御
赤松謙祐、鶴岡孝章、縄舟秀美
科学と工業 80 315 - 321 2006
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：a
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Linear double-stranded DNA that mimics an infective tail of virus genome to enhance transfection
T Anada, R Karinaga, K Koumoto, M Mizu, T Nagasaki, Y Kato, K Taira, S Shinkai, K Sakurai
JOURNAL OF CONTROLLED RELEASE 108 ( 2-3 ) 529 - 539 2005.11
Publisher：ELSEVIER SCIENCE BV
Our previous work showed that a natural beta-(1 -> 3)-D-glucan schizophyllan (SPG) can form a stable complex with single-stranded oligonucleotides (ssODNs). When protein transduction peptides were attached to SPG and this modified SPG was complexed with ssODNs, the resultant complex could induce cellular transfection of the bound ODNs, without producing serious cytotoxicity. However, no technique was available to transfect double-stranded DNAs (dsDNA) or plasmid DNA using SPG. This paper presents a new approach to transfect dsDNA, showing preparation and transfection efficiency for a minimal-size gene having a loop-shaped poly(dA)(80) on both ends. This poly(dA) loops of dsDNA can form a complex with SPG. An siRNA-coding dsDNA with the poly(dA) loop was complexed with Tat-attached SPG to silence luciferase expression. When LTR-Luc-HeLa cells that can express luciferase under the control of the LTR promoter were exposed to this complex, the expression of luciferase was suppressed (i.e., RNAi effect was enhanced). Cytotoxicity studies showed that the Tat-SPG complex induced much less cell death compared to polyethylenimine, indicating that the proposed method caused less harm than the conventional method. The Tat-SPG/poly(dA) looped dsDNA complex had a structure similar to the viral genome in that the dsDNA ends were able to induce transfection and protection. The present work identifies the SPG and poly(dA) looped minimum-sized gene combination as a candidate for a non-toxic gene delivery system. (c) 2005 Elsevier B.V. All rights reserved.
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Cholesterol-appended beta-(1 -> 3)-D-glucan schizophylllan for antisense oligonucleotides delivery to enhance the cellular uptake
K Koumoto, M Mizu, T Anada, T Nagasaki, S Shinkai, K Sakurai
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 78 ( 10 ) 1821 - 1830 2005.10
Publisher：CHEMICAL SOC JAPAN
Schizophyllan is a natural beta-(1 -> 3)-D-glucan existing as a triple helix in water and as a single chain in dimethyl sulfoxide (DMSO), respectively. As we already reported, when a homo-polynucleotide is added to a schizophyllan solution. the single chain of schizophyllan forms a complex with the polynucleotide. One of the potential applications of this novel complex is an antisense-oligonucleotide (AS ODN) carrier. The present paper describes a modification technique that enables us to introduce a cholesterol group only to the side chain of schizophyllan. We prepared four cholesterol-appended schizophyllans with different modification levels. Using these compounds, we made complexes and carried out an in vitro antisense assay, administrating a phosphorothioate AS ODN to the several cell lines to depress their c-myb mRNA. When we used 2.2-2.3 mol % modified schizophyllan as the carrier, the antisense effect was most enhanced among others. The addition of beta-cyclodextrin improved the complexation ability as well as the up-take for highly modified samples. Furthermore, the cytotoxicity for these modified schizophyllan samples was negligibly as small as the natural (unmodified) schizophyllan. The present work has thus clarified that schizophyllan can act as a new potential candidate for AS ODN carriers.
DOI： 10.1246/bcsj.78.1821
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一分子蛍光エネルギー移動（FRET）を利用したバイオ高分子の動的挙動解析
川上純司
高分子 54 ( 9 ) 703 2005.9
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：高分子学会
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PEG-appended beta-(1 -> 3)-D-glucan schizophyllan to deliver antisense-oligonucleotides with avoiding lysosomal degradation
R Karinaga, K Koumoto, M Mizu, T Anada, S Shinkai, K Sakurai
BIOMATERIALS 26 ( 23 ) 4866 - 4873 2005.8
Publisher：ELSEVIER SCI LTD
Schizophyllan is a natural beta-(1 -> 3)-D-glucan existing as a triple helix in water and as a single chain in dimethylsulfoxide (DMSO). As we already reported, when a homo-polynucleotide [e.g., poly(dA) or poly(C)] is added to the schizophyllan/DMSO solution and subsequently DMSO is exchanged for water, the single chain of schizophyllan forms a complex with the polynucleotide. One of the potential applications for this novel complex is an antisense-oligonucleotide (AS ODN) carrier. The present paper describes a modification technique that enabled us to introduce PEG only to the side chain of schizophyllan. This technique consisted of periodate oxidation of the glucose side chain and subsequent reaction between methoxypolyethylene glycol amine and the formyl terminate, followed by reduction with NaBH4. Subsequently, we made a complex from PEG-appended schizophyllan and an AS ODN sequence, and carried out an in vitro antisense assay, administrating the AS ODN complex to depress A375 c-myb mRNA of A375 melanoma cell lines. The PEG-SPG/AS ODN complex showed more enhanced antisnese effect than naked AS ODN dose, i.e., the same level as that of RGD-appended SPG. Here, the RGD system has been shown one on the most effective AS ODN carrier (Science 261 (1993) 1004-1012). When we added nigericin to the assay system, the antisense effect was not affected in the PEG-SPG system, on the other hand, it was almost eliminated in the RGD system. Nigericin is well known to interrupt transport from endosome to lysosome. Therefore, the difference between the PEG and RGD complexes indicates that, in the PEG system, AS ODN was able to escape from lysosomal degradation. The present work has thus proposed a new strategy to delivery AS ODN using schizophyllan as a new carrier. (C) 2004 Elsevier Ltd. All rights reserved.
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Parallel vs. anti-parallel orientation in a curdlan/oligo(dA) complex as estimated by a FRET technique
M Numata, K Koumoto, M Mizu, K Sakurai, S Shinkai
ORGANIC & BIOMOLECULAR CHEMISTRY 3 ( 12 ) 2255 - 2261 2005.6
Publisher：ROYAL SOC CHEMISTRY
We already found that beta-1,3-glucan polysaccharides form polymeric complexes with certain polynucleotides, but the parallel vs. anti-parallel orientation in those complexes had remained unsolved. In this paper, this controversial problem has been discussed for curdlan/oligo(dA) complexes utilizing two different energy transfer techniques. The first system consists of a combination of fluorescein-labeled curdlan and 3'-(or 5'-)tetramethyl-rhodamine (TAMRA)-labeled oligo(dA). The second system utilizes gold nanoparticles: that is, two curdlan chains were linked by a disulfide bond and after complexation with oligo(dA), the complex was immobilized on gold nanoparticles. In this system, TAMRA was attached to the 3' (or 5') end of oligo(dA) and the gold particle acted as a fluorescence quencher (energy acceptor). These experiments have led us to conclude that in the curdlan/oligo(dA) complex, parallel orientation is more favourable than anti-parallel orientation. These findings have enabled us to envision a clearer image for the complexation mode between beta-1,3-glucan polysaccharides and polynucleotides.
DOI： 10.1039/b500156k
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Construction of a PNA-DNA Hybrid Array Potential for a Novel Addressable Chip Reviewed
SANO Shusuke, USUI Kenji, TOMIZAKI Kin-ya, MIHARA Hisakazu
2004 387 - 388 2005.3
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Peptide Microarrays Using Designed Libraries for Protein Detection Reviewed
MIHARA Hisakazu, USUI Kenji, OJIMA Tetsunori, SUZUKI Masato, WATANABE Sin-ya, TOMIZAKI Kin-ya, NOKIHARA Kiyoshi
2004 113 - 114 2005.3
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Gene delivery system using polysaccharide schizophyllan and poly(DA) tailed DNA.
T Anada, R Karinaga, K Koumoto, M Mizu, S Shinkai, K Sakurai
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 229 U1160 - U1160 2005.3
Publishing type：Research paper, summary (international conference) Publisher：AMER CHEMICAL SOC
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In situ monitoring of polysaccharide-polynucleotide interaction using a schizophyllan-immobilized QCM device
DH Yang, AH Bae, K Koumoto, SW Lee, K Sakurai, S Shinkai
SENSORS AND ACTUATORS B-CHEMICAL 105 ( 2 ) 490 - 494 2005.3
Publisher：ELSEVIER SCIENCE SA
Schizophyllan belongs to the beta-(1 -> 3)-D-glucan family and is well known as a triple helix polysaccharide. It is already known that the single chain of schizophyllan (s-SPG) can specifically form a complex with particular polynucleotides. As an application of this recognition capability, a novel schizophyllan derivative connected with a disulfide bond through along methylene spacer was synthesized, and its interaction with polynucleoticles was directly monitored with a self-assembled monolayer on QCM. The gold-coated QCM electrode was covered in ca. 60% as a monolayer of s-SPG. The QCM resonator showed sensitive mass increases for poly(C) and poly(U) in the presence of K+ ion, which are consistent with our previous results. The present results indicate that the schizophyllan-immobilized QCM resonator can be a new sensing device for biomolecules. (c) 2004 Elsevier B.V. All rights reserved.
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Studies on Fluorescent Dyes and Surface Chemistry focusing on Practical Peptide Array Preparation
NOKIHARA Kiyoshi, USUI Kenji, YONEMURA Koichi, OHYAMA Takafumi, OKA Yasuo, TOMIZAKI Kin‐ya, MIHARA Hisakazu
Pept Sci 2004 145 - 148 2005.3
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Competition between polysaccharide/polynucleotide complexation vs., polynucleotide hybridization; salt concentration dependence of the reaction direction
Ryouji Karinaga, Kazuya Koumoto, Jusaku Minari, Masami Mizu, Takahisa Anada, Kazuo Sakurai, Seiji Shinkai
e-Journal of Surface Science and Nanotechnology 3 38 - 45 2005.2
Schizophyllan is a natural β-(1→3)-D-glucan existing as a triple helix in water and as a single chain in dimethylsulfoxide (DMSO), respectively. As we already reported, when a homo-polynucleotide [ex. poly (A) or poly(dA)] was added to the schizophyllan/DMSO solution and subsequently DMSO was exchanged for water, the single chain of schizophyllan formed a complex with the polynucleotide. In this paper, using an adenine-thymine (or uracil) double strand as a model system, we explored whether s-SPG can unzip the polynucleotide duplexes, examining the competition between the schizophyllan/polynucleotide complexation and the polynucleotide hybridization with circular dichroism and fluorescence spectroscopy at various NaCl concentrations. The unzipping reaction happened for the hetero-duplexes (i.e., RNA-DNA duplexes) at low salt concentrations, on the other hand, it was not observed for the homo-duplexes at all the salt concentrations. When we compared dissociation temperatures, the unzipping reaction of the homo-duplexes was expected at low salt concentrations. This discrepancy between the experimental results and the expectation, as well as the different behavior between the hetero- and homo- duplexes, can be ascribed to the difference in the activation energy of intermediate states. © 2005 The Surface Science Society of Japan.
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テーラーメード・リボスイッチ
川上純司
高分子 54 ( 2 ) 93 2005.2
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：高分子学会
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Poly(N-substituted-acrylamide)-branched schizophyllans are useful for selective recovery of homopolynucleotides through convenient and quick precipitation procedures
T Matsumoto, M Umeda, M Numata, T Hasegawa, K Sakurai, K Koumoto, S Shinkai
POLYMER JOURNAL 37 ( 3 ) 177 - 185 2005
Publisher：SOC POLYMER SCIENCE JAPAN
Schizophyllans carrying poly(N-substituted-acrylamide)-branches were synthesized from native schizophyllan through Ce(IV)-initiated radical homo- or co-polymerization of N-iso-propyl- and N-sec-butyl-acrylamide. A series of structural analyses including elemental analysis, gel permeation chromatography, H-1 NMR, and enzymatic degradation using β-1,3-glucanase revealed that schizophyllans (150kDa) bearing long poly (N-substituted-acrylamide)-branches (ca. 290 kDa) are obtained. These SPG-conjugates form macromolecular complexes with certain homopolynucleotides such as poly(C), poly(dA), and poly(A) and then efficiently co-precipitate them on heating. For example, schizophyllan carrying poly(N-iso-propylacrylamide)-branches can selectively recover poly(C) from a mixture of poly(C) and poly(U) by simple heating (36° C) followed by centrifugation. HPLC analysis of the recovered polynucleotide revealed the high poly(C)-selectivity (poly(C):poly(U) = 95:5). Similarly, schizophyllan carrying more hydrophobic poly(N-iso-propylacrylamide-co-N-sec-butylacrylamide) -branches precipitates at lower temperature (ca. 20° C) and is applicable to isolation of poly(A) that forms less stable macromolecular complexes with SPG and SPG-derivatives (the dissociation temperatures are around 30° C).
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Transferrin-appended β-(1→3)-D-glucan schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake
Takahisa Anada, Ryouji Karinaga, Masami Mizu, Kazuya Koumoto, Takahiro Matsumoto, Munenori Numata, Seiji Shinkai, Kazuo Sakurai
e-J. Surface Sci. Nanotech. 3 195 - 202 2005
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An SPR Sensor Chip for Sensitive Detection of Small Molecules Using Molecularly Imprinted Polymer with Embedded Gold Nanoparticle
SUGIMOTO Naoki, AKAMATSU Kensuke, HARA Noriaki, MIYOSHI Daisuke, NAWAFUNE Hidemi, TAMAKI Katsuyuki, MATSUI Jun
Anal. Chem. 77 ( 13 ) 4282 - 4285 2005
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ナノテクノロジーの表面技術への展開
縄舟秀美、赤松謙祐、池田慎吾
表面技術 56 730 - 737 2005
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：表面技術協会
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ナノサイズ微粒子を利用した微細配線技術
縄舟秀美、赤松謙祐
日本金属学会誌 69 179 - 189 2005
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：日本金属学会
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量子ドット（Q-dot）の基礎とナノバイオエンジニアリング
赤松謙祐、鶴岡孝章、縄舟秀美、杉本直己
細胞工学 24 60 - 65 2005
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：学研メディカル秀潤社
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微細配線形成における湿式めっき法のプロセス・イノベーション
縄舟秀美、赤松謙祐、池田慎吾
科学と工業 79 34 - 40 2005
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：大阪工研協会
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化学的表面処理によるポリイミド樹脂のダイレクト・メタラリゼーション
赤松謙祐、池田慎吾、縄舟秀美
日本接着学会誌 41 325 - 330 2005
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：日本接着学会
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金属ナノ粒子分散ハイブリッド薄膜の調製
赤松謙祐、池田慎吾、縄舟秀美
化学工業 56 40 - 46 2005
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：a
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光触媒反応を利用したダイレクトパターニング
赤松謙祐、池田慎吾、縄舟秀美
表面技術 56 665 - 669 2005
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：表面技術協会
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A novel fabrication technique for interacting ferromagnetic-metal nanoparticle systems:fine-tuning of particle dimeter and interparticle spacing
S. Tomita, K. Akamatsu, H. Shinkai, S. Ikeda, H. Nawafune, C. Mitsumata, T. Kashiwagi, M. Hagiwara
Mater. Res. Soc. Proceedings 853E I5.10.1-I5.10.6 2005
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Band gap engineering of CdTe nanocrystals through chemical surface modification
K. Akamatsu, T. Tsuruoka, H. Nawafune
J. Am. Chem. Soc. 127 ( 6 ) 1634 - 1635 2005
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Controlling interparticle spacing among metal nanoparticles through metal-catalyzed decomposition of surrounding polymer matrix
K. Akamatsu, H. Shinkai, S. Ikeda, S. Adachi, H. Nawafune, S. Tomita
J. Am. Chem. Soc. 127 ( 22 ) 7980 - 7981 2005
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Tuning magnetic interactions in ferromagnetic-metal nanoparticle systems
S. Tomita, K. Akamatsu, H. Shinkai, S. Ikeda, H. Nawafune, C. Mitsumata, T. Kashiwagi, and M, Hagiwara
Phys. Rev. B 71 180414(R)1-4 2005
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Synthesis and structural control of metal-containing polyimide nanocomposite
H. Shinkai, S. Ikeda, K. Akamatsu, H. Nawafune, S. Tomita
Trans. Mater. Res. Soc. Jpn. 30 1223 - 1226 2005
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Surface functionalization of mixed monolayer-protected semiconductor nanocrystals
T. Tsuruoka, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. 30 1227 - 1230 2005
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Stereocontrolled synthesis and optical properties of all-cis poly(phenylene vinylene)s (PPVs): A method for direct patterning of PPVs
H. Katayama, M. Nagao, T. Nishimura, Y. Matsui, K. Umeda, K. Akamatsu, T. Tsuruoka, H. Nawafune, F. Ozawa
J. Am. Chem. Soc. 127 ( 12 ) 4350 - 4353 2005
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Site-selective direct photochemical deposition of copper on glass substrates using TiO2 nanocrystals
K. Akamatsu, A. Kimura, H. Matsubara, S. Ikeda, H. Nawafune
Langmuir 21 ( 18 ) 8099 - 8102 2005
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Site-selective deposition of gold on glass substrates using monolayer-protected gold nanoparticles
K. Akamatsu, H. Terahata, J. Hasegawa, H. Nawafune
J. Surf. Finish. Soc. Jpn. 56 ( 7 ) 427 - 428 2005
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Selective Electroless Copper Deposition on Aluminum Nitride Substrate with Patterned Copper Seed Layer
Thin Solid Films 491 ( 1-2 ) 18 - 22 2005
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Fabrication and structural control of Fe/Ti oxide thin films with graded compositional profiles by liquid phase deposition
S. Deki, S. Iizuka, K. Akamatsu, M. Mizuhata and A. Kajinami
J. Am. Ceram. Soc. 88 ( 3 ) 731 - 736 2005
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Amino Acid Ester Salt Recognition by Ferrocene-based Ditopic Receptor Bearing Oligoethylene Glycol with Pendant Bipy Subunits: CV, UV-vis and ESR Studies.
Org. Biomol. Chem. 3, 4060-4064 2005
DOI： 10.1039/b511233h
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Synthesis of water-soluble porphyrin and the corresponding highly planar benzoporphyrin without meso-substituents.
Tetrahedron Lett., 46, 113-116 2005
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A protein detection system with designed peptides using anomalous reflection of gold
S Watanabe, K Usui, K Tomizaki, H Mihara, M Watanabe, K Kajikawa
BIOPOLYMERS 80 ( 4 ) 599 - 599 2005
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS INC
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Designed peptide microarrays for protein detection and characterization
H Mihara, K Usui, K Tomizaki, K Nokihara
BIOPOLYMERS 80 ( 4 ) 493 - 493 2005
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS INC
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alpha-helical peptide microarrays using a dry peptide array method
K Usui, K Tomizaki, K Nokihara, H Mihara
BIOPOLYMERS 80 ( 4 ) 599 - 599 2005
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS INC
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Protein-Detecting Microarrays: Current Accomplishments and Requirements
K.-Y. Tomizaki, K. Usui and H. Mihara
ChemBioChem 6 782 - 799 2005
Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：WILEY-VCH
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Construction of Protein-Detection System using anomalous reflection of gold Reviewed
渡辺晋也, 臼井健二, 富崎欣也, 渡辺光昭, 梶川浩太郎, 三原久和
日本化学会講演予稿集 85th ( 2 ) 2005
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量子ドット（Q-dot）の基礎とナノバイオエンジニアリング
赤松謙祐、鶴岡孝章、縄舟秀美、杉本直己
細胞工学 24 60 - 65 2005
Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：学研メディカル秀潤社
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Removal of the side-chain glucose groups from schizophyllan improves the thermal stability of the polycytidylic acid complexes under the physiological conditions
K Koumoto, R Karinaga, M Mizu, T Anada, K Sakurai, T Kunitake, S Shinkai
BIOPOLYMERS 75 ( 5 ) 403 - 411 2004.12
Publisher：JOHN WILEY & SONS INC
Thermal stabilization of the complex between polycytidylic acid [poly(Q] and the modified schizophyllan (SPG) whose hydrophilic side-chain glucose groups are selectively removed utilizing mild Smith-degradation has been investigated. With the decrease in the side-chain glucose groups of schizophyllan, the complex with poly(C) can be considerably stabilized compared with unmodified SPG; for example, the T. value after the removal of the side-chain glucose groups from 33.3 (unmodified) to 1.0 is enhanced by 14degreesC In addition, the thermal stabilization effect is even operative under the physiological conditions ([NaCl] = 0.15 mol dm(-3)). This effect is exerted owing to the construction of the hydrophobic atmosphere around the complex. Although schizophyllan lost the side-chain glucose groups, it still kept the protection effect of the bound poly(C) chain against RNaseA-mediated hydrolysis as observed for unmodified schizophyllan. The assessment of the cytotoxicity for A375:human malignant melanoma, and HL60:human promyelocytic leukemia revealed that the modified schizophyllan scarcely increases the cytotoxicity. These results indicate that the present modification for schizophyllan is of great significance in a viewpoint to develop the practical gene carriers operative even under the physiological conditions. (C) 2004 Wiley Periodicals, Inc.
DOI： 10.1002/bip.20148
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Proposal of new modification technique for linear double-stranded DNAs using the polysaccharide schizopyllan
T Anada, H Matsunaga, R Karinaga, K Koumoto, M Mizu, K Nakano, S Shinkai, K Sakurai
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 14 ( 22 ) 5655 - 5659 2004.11
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA)(80) tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture, that is, quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation. (C) 2004 Elsevier Ltd. All rights reserved.
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A polysaccharide carrier for immunostimulatory CpG DNAs to enhance cytokine secretion
M Mizu, K Koumoto, T Anada, T Matsumoto, M Numata, S Shinkai, T Nagasaki, K Sakurai
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 126 ( 27 ) 8372 - 8373 2004.7
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Protection of polynucleotides against nuclease-mediated hydrolysis by complexation with schizophyllan
M Mizu, K Koumoto, T Kimura, K Sakurai, S Shinkai
BIOMATERIALS 25 ( 15 ) 3109 - 3116 2004.7
Publisher：ELSEVIER SCI LTD
Schizophyllan is a beta-(1-->3)-D-glucan existing as a triple helix in water and as a single chain in dimethylsulfoxide (DMSO), respectively. As we already reported, when some homo-polynucleotide (for example, poly(dA) or poly(C)) is added to the schizophyllan/DMSO solution and subsequently DMSO is exchanged for water, the single chain of schizophyllan (s-SPG) forms a complex with the polynucleotide. The present work demonstrates that the polynucleotide bound in the complex is more stable to nuclease-mediated hydrolysis than the polynucleotide itself (i.e., naked polynucleotide), using high-performance liquid chromatography and ultraviolet absorbance technique. A kinetic study for the hydrolysis clarified that the simple Michaelis-Menten relation is held and the maximum velocity for the complex is one-sixth as small as that of the naked polynucleotide. This low hydrolysis rate for the complex suggests that s-SPG is applicable to a carrier for antisense oligonucleotides. (C) 2003 Elsevier Ltd. All rights reserved.
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Antisense oligonucleotides bound in the polysaccharide complex and the enhanced antisense effect due to the low hydrolysis
M Mizu, K Koumoto, T Anada, K Sakurai, S Shinkai
BIOMATERIALS 25 ( 15 ) 3117 - 3123 2004.7
Publisher：ELSEVIER SCI LTD
Schizophyllan is a beta-(1--> 3)-D-glucan and can form a novel complex with some single-chains of DNAs. As the preceding paper revealed, the polynucleotide bound in the complex is more stable to nuclease-mediated hydrolysis than the polynucleotide itself (i.e., naked polynucleotide). This paper examined possibility to apply this complex to an antisense DNA carrier, using an in vitro (cellfree) transcription/translation assay. In this assay, we used a plasmid DNA coding a green fluorescence protein (GFP) and an antisense DNA designed to hybridize the ribosome-binding site in the GFP-coded mRNA. When the antisense DNA was administered as the complex, a lower GFP expression efficiency (or higher antisense effect) is observed over naked DNA. This is because the antisense DNA in the complex is protected from the attack of deoxyribonuclease. When exonuclease I, which specifically hydrolyzes single DNA chains, was present in the GEP assay system, the antisense effect was not changed for the complex while being weakened in the naked antisense DNA system. These results imply that the exonuclease I cannot hydrolyze the antisense DNA in the complex, while it can hydrolyze naked DNA to reduce its antisense effect. (C) 2003 Elsevier Ltd. All rights reserved.
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『T細胞におけるアダプター分子3BP2の機能解析』(平成15(2003)年度公開セミナー : 老研研究発表会(平成15年1月〜12月))
川内 敬子
日本医科大学老人病研究所紀要 10 2004.3
Publisher：日本医科大学
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Long-term expression with a cationic polymer derived from a natural polysaccharide: Schizophyllan
T Nagasaki, M Hojo, A Uno, T Satoh, K Koumoto, M Mizu, K Sakurai, S Shinkai
BIOCONJUGATE CHEMISTRY 15 ( 2 ) 249 - 259 2004.3
Publisher：AMER CHEMICAL SOC
Among the various synthetic gene carriers based on biomaterials, cationic polymers with polysaccharide backbones have long been studied as nonviral vectors due to their low immunogenicity and high water solubility. Schizophyllan, a beta-(1,3)-glucan, is one of the various polysaccharides that are clinically administered. Furthermore, its safety in the human body has already been confirmed. Various functional groups can be selectively introduced into the side chain, not into the main chain of schizophyllan. Therefore, we have synthesized various oligoamine conjugates from schizophyllan. It was confirmed that their in vitro transfection efficiencies are superior to that of polyethylenimine by adjusting the molecular weight and the degree of amination of cationic schizophyllan. While it was possible to reduce cytotoxicity by adjusting the amount of DNA complex per cell, as seen with poly-L-lysine, polyethylenimine, and chitosan, PEGylation was the most effective means of reducing toxicity. Furthermore, using cationic schizophyllan carriers, it was also possible to express a reporter protein for a long period of time due to a long residence time of plasmid DNA in cells.
DOI： 10.1021/bc034178x
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Lactose-appended schizophyllan is a potential candidate as a hepatocyte-targeted antisense carrier
T Hasegawa, M Umeda, T Matsumoto, M Numata, M Mizu, K Koumoto, K Sakurai, S Shinkai
CHEMICAL COMMUNICATIONS ( 4 ) 382 - 383 2004.2
Publisher：ROYAL SOC CHEMISTRY
A schizophyllan (beta-1,3-glucan) derivative carrying lactose-appendages prepared by reductive amination can form stable macromolecular complexes with polynucleotides, shows excellent affinity with a lactose-binding lectin, and effectively mediates gene transfection into hepatocytes.
DOI： 10.1039/b313426a
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Chemically modified polysaccharide schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake efficiency
T Matsumoto, M Numata, T Anada, M Mizu, K Koumoto, K Sakurai, T Nagasaki, S Shinkai
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1670 ( 2 ) 91 - 104 2004.1
Publisher：ELSEVIER SCIENCE BV
Schizophyllan is a natural beta-(1 --> 3)-D-glucan existing as a triple helix in water and as a single chain in dimethylsulfoxide (DMSO), respectively. As we already reported, when some homo-phosphodiester polynucleotide (for example, poly(dA) or poly(C)) is added to the schizophyllan/DMSO solution and subsequently DMSO is exchanged for water, the single chain of schizophyllan forms a complex with the polynucleotide. Furthermore, we have already demonstrated that one of the potential applications of this novel complex is an antisense-oligonucleotide (AS ODN) carrier. This work describes a versatile and universal modification technique which enables us to introduce various functional groups only to the side chain of schizophyllan. This technique consists of periodate oxidation of the glucose side chain (it does not react with the main chain because of the absence of the 1,2-diol group in beta-(1 --> 3)-glucan) and subsequent introduction of the functional groups into the formyl terminate. In the present work, the introduced functional groups were spermine, octa-arginine (R8), arginine-glycine-aspartic acid tripeptide (RGD) and some amino or alpha-amino acid compounds. Using these compounds, we made the complexes and carried out an in vitro antisense assay for them, administrating a phosphorothioate AS ODN to the melanoma A375 or leukemia HL-60 cell lines to depress their c-myb mRNA. When we used the R8 or RGD modified schizophyllan as the antisense carrier, the antisense effect was most enhanced among others. Their superiority can be ascribed to enhancement of endocytosis due to these functional peptides. Furthermore, the cytotoxicity for these two modified schizophyllans was negligibly as small as the natural (unmodified) schizophyllan. One of the peculiar features of our system is that the complex (i.e., carrier + AS ODN) is charged negatively in total, which is different from the conventional systems. The present work has thus clarified that schizophyllan can act as a new potential candidate for AS ODN carriers. (C) 2004 Elsevier B.V. All rights reserved.
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Low M-W sulfated curdlan with improved water solubility forms macromolecular complexes with polycytidylic acid
K Koumoto, M Umeda, M Numata, T Matsumoto, K Sakurai, T Kunitake, S Shinkai
CARBOHYDRATE RESEARCH 339 ( 1 ) 161 - + 2004.1
Publisher：ELSEVIER SCI LTD
The water solubility of curdlan was enhanced by partial sulfation at O-6. Circular dichroism measurements suggest that the sulfated curdlan with the degree of substitution (DS) from 0 to 8.7 mol % forms macromolecular complexes with polycytidylic acid (poly(C)). Although the thermal stability of the complexes decreased with increase in DS, this could be overlapped by addition of NaCl in the concentration above that of serum. The results clearly indicate that the drawback arising from the electrostatic repulsion between the anionic charges can be partially compensated by the presence of salt. Furthermore, the polynucleotide chain complexed with the sulfated curdlan was protected from the enzymatic hydrolysis, corroborating the assumption that the sulfated curdlan has an ability to bind poly(C). (C) 2003 Elsevier Ltd. All rights reserved.
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Catalytic roles for proton transfer and protonation in ribozymes: Current understanding and future possibilities
P. C. Bevilacqua, T. S. Brown, S. Nakano, and R. Yajima
Biopolymers 73 ( 1 ) 90 - 109 2004.1
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：Wiley
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Enhancement of antisense effect by reducing interactions between proteins and ODNs
Masami Mizu, Kazuya Koumoto, Takahisa Anada, Ryouji Karinaga, Taro Kimura, Kazuo Sakurai, Takeshi Nagasaki, Seiji Shinkai
Bull. Chem. Soc. Jpn. 77 1101 - 1110 2004
DOI： 10.1246/bcsj.77.1101
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Schizophyllans carrying oligosaccharide appendages as potential candidates for cell-targeted antisense carrier
T Hasegawa, T Fujisawa, M Numata, T Matsumoto, M Umeda, R Karinaga, M Mizu, K Koumoto, T Kimura, S Okumura, K Sakurai, S Shinkai
ORGANIC & BIOMOLECULAR CHEMISTRY 2 ( 21 ) 3091 - 3098 2004
Publisher：ROYAL SOC CHEMISTRY
Schizophyllans carrying beta-lactoside and alpha-mannoside appendages were prepared from native schizophyllan through NaIO4 oxidation followed by reductive amination using aminoethyl-beta-lactoside and alpha-mannoside, respectively. The resulting schizophyllans form stable macromolecular complexes with polynucleotides, such as poly(C) and poly(dA). Specific affinity between these macromolecular complexes and saccharide-binding proteins was demonstrated by surface plasmon resonance and agarose gel staining assays. beta-Lactoside-appended schizophyllan enhanced an antisense activity in hepatocytes which express lactoside-binding proteins on their cell surfaces.
DOI： 10.1039/b412124b
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Polysaccharide/polynucleotide complexes. Part 6. Complementary-strand-induced release of single-stranded DNA bound in the schizophyllan complex
K Koumoto, M Mizu, K Sakurai, T Kunitake, S Shinkai
CHEMISTRY & BIODIVERSITY 1 ( 3 ) 520 - 529 2004
Publisher：VERLAG HELVETICA CHIMICA ACTA AG
Spectroscopic properties of single-stranded DNA/schizophyllan ternary complexes (ss-DNA - 2s-SPG), induced by addition of either complementary or noncomplementary strands, have been investigated. The addition of the complementary strands to ss-DNA - 2s-SPG induced the quick release of the bound ss-DNA to the complementary strands (both DNA and RNA), whereas the ternary complex was unaffected upon addition of noncomplementary strands. Our experiments imply that SPG has complexation properties indispensable to the gene carriers. As far as we know, there is no report on exploitation of such nonviral gene carriers that can accomplish an intelligent release of the bound ss-DNA toward the complementary strands. We believe, therefore, that SPG, a natural and neutral polysaccharide, has a great potential to become a new ss-DNA carrier.
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Molecular weight control of curdlan (beta-1,3-glucan polysaccharide) provides unique polynucleotide binding properties
K Koumoto, H Kobayashi, M Mizu, T Kimura, K Sakurai, T Kunitake, S Shinkai
POLYMER JOURNAL 36 ( 5 ) 380 - 385 2004
Publisher：SOC POLYMER SCIENCE JAPAN
Recently, we found that schizophyllan belonging to a beta-1,3-glucan polysaccharide family forms macromolecular complexes with certain single-stranded polynucleotides. The specific complex formation is originated from the beta-1,3-glucan skeleton in the main chain. In this paper, we have reported that curdlan, which is a beta-1,3-glucan polysaccharide without a side chain glucose unit, shows unique poly(C) binding properties different from those of schizophyllan; in the experiments using curdlan samples with the controlled molecular weight, the complexation ability appeared only in a limited "M-w range window". Stoichiometric analysis revealed that the "M-w range window" appears as a result of the competition between curdlan-curdlan self-association and curdlan-polynucleotide complexation. Furthermore, when the experiments were extended to other polynucleotides such as poly(A), poly(dA) and poly(dT), the "M-w range window" also appeared at their characteristic molecular weights. We believe, therefore, that curdlan can be applicable, in spite of its cheapness, as a precious functional material to gene technology.
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First observation by fluorescence polarization of complexation between mRNA and the natural polysaccharide schizophyllan
R Karinaga, M Mizu, K Koumoto, T Anada, S Shinkai, T Kimura, K Sakurai
CHEMISTRY & BIODIVERSITY 1 ( 4 ) 634 - 639 2004
Publisher：VERLAG HELVETICA CHIMICA ACTA AG
Schizophyllan is a natural beta-(1 --> 3)-D-glucan that exists as a triple helix in H2O and as a single chain in dimethylsulfoxide (DMSO) or basic solution (pH > 13). As we have already reported, when a homopolynucleotide (e.g., poly(dA), poly(A), or poly(C)) is added to a schizophylian/DMSO solution, and, subsequently, DMSO is exchanged for H2O, the single chain of schizophyllan forms a complex with the polynucleotide. Since eukaryotic mRNAs have poly(A) tails, we hypothesized that schizophyllan can bind to mRNA by interacting with this tail. However, we have not yet observed complexation between schizophyllan and mRNA after exchanging DMSO for H2O. In this report, we show that the complexation can be accelerated when the solution pH is changed from 13 to 7-8 in the presence of schizophyllan and polynucleotides. By this approach, we found that schizophyllan forms a complex with a yeast mRNA.
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Enhancement of the Antisense Effect of Polysaccharide-Polynucleotide Complexes by Preventing the Antisense Oligonucleotide from Binding to Proteins in the Culture Medium
Masami MIZU, Kazuya KOUMOTO, Takahisa ANADA, Ryouji KARINAGA, Taro KIMURA, Takeshi NAGASAKI, Seiji SHINKAI, Kazuo SAKURAI
Bull. Chem. Soc. Jpn. 77 ( 6 ) 1101 - 1110 2004
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多糖・核酸複合体を用いた機能性高分子コンジュゲート −核酸医薬デリバリーへの挑戦−
狩長亮二, 甲元一也, 櫻井和朗, 新海征治
高分子加工 53 561 - 568 2004
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Composite of Au Nanoparticles and molecularly imprinted polymer as a sensing material
SUGIMOTO N, AKAMATSU K, NISHIGUCHI S, MIYOSHI D, NAWAFUNE H, TAMAKI K, MATSUI J
Anal. Chem. 76 ( 5 ) 1310 - 1315 2004
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銅ナノ粒子分散ポリイミド樹脂と銅微細配線のナノスケール接合
縄舟秀美、赤松謙祐、池田慎吾
マテリアルステージ 3 58 - 62 2004
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：技術情報協会
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Aqueous solution-based synthesis of rare earth-doped metal oxide thin films
Thin Solid Films 460 ( 1/2 ) 83 - 86 2004
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Direct Patterning of Copper on Plymide Using Ion Exchangeable Surfece Templates Generated by Site-Selective Surface Modification
J. Am. Chem. Soc. 126 ( 35 ) 10822 - 10823 2004
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Syntesis, Surface Modification and Multilayer Construction of Mixed Monolayer-Protected CdS Nanoparticles
Langmuir 20 ( 25 ) 11169 - 11174 2004
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Preparation of transparent alumina film and fiber from a composite of aluminum polynuclear complex/methy cellulose
Mater. Chem. Phys. 83 ( 1 ) 54 - 59 2004
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Polyaddition of 2,7-diethyl-9, 9-dioctryfluorene using regio- andstereoselective alkyne dimerization catalysts
Macromolecules 37 ( 1 ) 13 - 17 2004
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Foramtion and Growth of Copper Nanoparticles from Ion-Doped Precursor Polymide Layers
J. Phys. Chem. B. 108 ( 40 ) 15599 - 15607 2004
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Electroless Copper Plating Using Sn(Ⅱ) Complex as a Reducing Agent for ULSI Metallization
AKAMATSU Kensuke
Mem. Konan Univ., Sci. Eng. Ser. 51, 29-38 2004
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Electroless copper plating using Sn(II) complex as a reducing agent for ULSI metallization
H. Nawafune, Y. Kobata, K. Akamatsu, S. Mizumoto, Y. Murakami
Mem. Konan Univ., Sci. Eng.Ser. 51 29 - 38 2004
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銅ナノ粒子分散ポリイミド樹脂と銅微細配線のナノスケール接合
赤松 謙祐
マテリアルステージ 3, 58-672 2004
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A novel intramolecular hydrogen bonding between a side-chain pyridine ring and an amide hydrogen of the peptide backbone in tripeptides containing the new amino acid, a,a-di(2-pyridyl)glycine.
Org. Biomol. Chem., 2, 2335-2339 2004
DOI： 10.1039/b406545j
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Synthesis of Tripeptides Containing a Very Crowded a,a-Disubstituted Glycine with Pyridine Rings by Solid-Phase Ugi Reaction.
Tetrahedron Lett, 45, 8871-8874 2004
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OPTIMIZATION OF PEPTIDE ARRAYS ON A NOVEL PROTEIN CHIP FOR A PRACTICAL PROTEIN CHARACTERIZATION SYSTEM
K. Nokihara, T. Ohyama, K. Yonemura, N. Sugioka, K. Usui, T. Ojima, H. Mihara
JOURNAL OF PEPTIDE SCIENCE 10 116 - 116 2004
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS LTD
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Peptide microarrays using structure-based peptide libraries for protein chips
Kenji Usui, Mizuki Takahashi, Tetsunori Ojima, Masato Suzuki, Kiyoshi Nokihara, Eiichi Tamiya, Hisakazu Mihara
Peptide Revolution: Genomics, Proteomics & Therapeutics 258 - 259 2004
Publisher：AMER CHEMICAL SOC
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PROTEIN-DETECTION MICROARRAYS USING STRUCTURE-BASED DESIGNED PEPTIDE LIBRARIES
K. Usui, T. Ojima, M. Suzuki, S. Watanabe, K. Tomizaki, K. Nokihara, H. Mihara
JOURNAL OF PEPTIDE SCIENCE 10 158 - 158 2004
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS LTD
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ペプチドアレイを用いたプロテインチップの実用化を目指した研究 Reviewed
軒原清史・大山貴史・臼井健二・米村耕一・富崎欣也・三原久和
高分子論文集 61 523 - 532 2004
Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：高分子学会
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Construction of a Phosphopeptide Array for Kinase-related Protein Detection Reviewed
鈴木正人, 臼井健二, 富崎欣也, 三原久和
日本化学会講演予稿集 84th ( 2 ) 2004
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Construction of Protein-Detection System with Peptides Containing Nucleobase Amino Acids (NBAs) Reviewed
渡辺晋也, 臼井健二, 富崎欣也, 三原久和
日本化学会講演予稿集 84th ( 2 ) 2004
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Development of Peptide-Chips Focusing on a High Throughput Protein-Detection System Reviewed
K. Nokihara, T. Ohyama, K. Usui, K. Yoneyama, M. Takahashi and H. Mihara
Innovation and Perspectives in Solid Phase Synthesis & Combinatorial Libraries 83 - 88 2004
Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：Mayflower Worldwide Limited
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Development of a practical protein-chip using designed synthetic peptide-arrays Reviewed
K Nokihara, T Ohyama, K Usui, K Yonemura, K Tomizaki, H Mihara
KOBUNSHI RONBUNSHU 61 ( 10 ) 523 - 532 2004
Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：SOC POLYMER SCIENCE JAPAN
Novel high-throughput technologies, which can replace conventional electrophoresis and mass spectroscopic analyses, are in great demand for understanding the structure-function relationships of proteins. For a practical protein-detection system, arrays with immobilized designed synthetic peptides have been constructed. Peptides were labeled with fluorescent dyes in order to achieve high sensitivity. Two different types of prototype-arrayer have been constructed: one has a micro-dispensing system and the other a spot-printing system. Combinatorial peptide libraries, which consisted of beta-loop, beta-strand and alpha-helical peptides, were constructed by improved highly efficient solid-phase synthesis. Labeled peptides were covalently immobilized on solid supports such as precision glass plates. Various proteins were characterized with these peptide-arrays using a fluorescent scanner to give the "protein fingerprint" which is characteristic of the individual proteins. The results of the present prototype system demonstrate the practicality of protein-chips as a new generation of biochips.
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Microanalysis of nucleic acids using the limulus G test
T Anada, K Koumoto, M Mizu, R Karinaga, K Sakurai, S Shinkai
ANALYTICAL SCIENCES 19 ( 12 ) 1567 - 1568 2003.12
Publisher：JAPAN SOC ANALYTICAL CHEMISTRY
The limulus G test has been used as a quantitative analysis of (1-->3)-beta-D-glucans, including schizophyllan (SPG) and curdlan. The present work extended the limulus G test to detect polynucleotide/SPG complexes. The complex showed an extremely sensitive response to the test, compared with SPG itself. The minimum concentration of the complex to show the response is almost 10-times as small as that of SPG itself, indicating the possibility to detect (1-->3)-beta-D-glucans or/and polynucleotides on the pico gram/ml scale.
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Supramolecular structure of a sugar-appended organogelator explored with synchrotron X-ray small-angle scattering
K Sakurai, Y Jeong, K Koumoto, A Friggeri, O Gronwald, S Sakurai, S Okamoto, K Inoue, S Shinkai
LANGMUIR 19 ( 20 ) 8211 - 8217 2003.9
Publisher：AMER CHEMICAL SOC
Small-angle X-ray scattering (SAXS) from an organogel system prepared from methyl 4,6-O-benzylidene-alpha-D-mannopyranoside and p-xylene was carried out with a synchrotron X-ray source at SPring-8, which revealed that hexagonally packed fibrils are formed in the gel state. The spacing between the fibrils can be evaluated to be about 60 Angstrom, and this value was almost independent of both the gelator concentration and the temperature. Furthermore, the spacing is larger than the gelator molecular size. Upon heating, this supramolecular structure completely disappeared. Time-resolved SAXS revealed that phase separation takes place initially and subsequently the hexagonal structure is formed. Wide-angle X-ray diffraction (WARD) showed that there is no crystalline peak at all and the diffraction pattern is consistent with being amorphous. H-1 NMR spectral data show that the gelator molecules still maintain thermal motion in the gel state. The present SAXS, WARD, and NMR results contrast with those of "dry gels" in which the gel fibers consist of the crystal of the gelators. Our results suggest that the solvent molecules are incorporated into the gel fiber and the present gel can be classified as a "wet gel".
DOI： 10.1021/la0346752
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ペプチドマイクロアレイ ～ペプチドライブラリを用いた新規プロテインチップの創製～
臼井健二,三原久和
Bioベンチャー 3 99 - 102 2003.8
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：羊土社
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遺伝子の正体：DNAとRNA
杉本直己・中野修一
化学と教育 51 ( 7 ) 398 - 401 2003.7
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：日本化学会
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Polysaccharide-polynucleotide complexes. Part 7. Hydrogen-ion and salt concentration dependence of complexation between schizophyllan and single-stranded homo RNAs
K Sakurai, R Iguchi, M Mizu, K Koumoto, S Shinkai
BIOORGANIC CHEMISTRY 31 ( 3 ) 216 - 226 2003.6
Publisher：ACADEMIC PRESS INC ELSEVIER SCIENCE
Schizophyllan belongs to a beta-1,3-D-glucan family, which exists as a random coil in dimethyl sulfoxide (DMSO) and as a triple helix in water, respectively. The schizophyllan single chain forms a complex with single-stranded homo RNAs in water/DMSO mixed solvents. Using circular dichroism, we studied the complexation and its stability as a function of apparent pH (pH*) in a mixed solvent system and as a function of the salt concentration. The complex is formed in the pH* range 6.5-10, and dissociated in the pH* range 4-6. Both poly(A) and poly(C) adopt a double strand in the pH* range 4-6 and a single strand in the pH* range 6.5-10. Therefore, the conformational change of each polynucleotide is responsible for dissociation/association of the complex, i.e., the single strand of the polynucleotides can form complexes, whereas the double one cannot. This result indicates that hydrogen bonding and similarity of the helix parameters are essential for the complex formation. The melting temperature of the complex reaches the maximum around 0.05 M of NaCl and KCl, and the value of the maximum temperature depends on the cation species. (C) 2003 Elsevier Science (USA). All rights reserved.
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Polysaccharide-polynucleotide complexes (15): thermal stability of schizophyllan (SPG)/poly(C) triple strands is controllable by alpha-amino acid modification
M Numata, T Matsumoto, M Umeda, K Koumoto, K Sakurai, S Shinkai
BIOORGANIC CHEMISTRY 31 ( 2 ) 163 - 171 2003.4
Publisher：ACADEMIC PRESS INC ELSEVIER SCIENCE
Schizophyllan (SPG), a beta-1,3-glucan polysaccharide which is known to form macromolecular complexes with certain polynucleotides, was modified by a reductive amination method with alpha-amino, acids (Arg, Lys, and Ser). The thermal stability of the complexes as estimated by T-m was enhanced in SPG-Arg and SPG-Lys conjugates which have pI values higher than the pH of the medium (8.0). The T-m shift increased with the increase in the percentage of alpha-amino acid introduced and the highest T-m values attained were 64 degreesC for SPG-Arg conjugate and 62 degreesC for SPG-Lys conjugate, which are higher by 13 and 11 degreesC, respectively, than those of the unmodified SPG + poly(C) complex. In the SPG-Ser conjugate with a pI lower than the medium pH (8.0), the T-m values decreased with an increase in the percentage of Ser. Formation of the macromolecular complex was no longer detected above 13.2% Ser. The findings indicate that the T-m values are easily controllable by the type and percentage of the introduced alpha-amino acids. We believe, therefore, that the present conjugates, consisting of naturally originated SPG and alpha-amino acids, provide an important lead for developing nontoxic artificial vectors and to control the affinity with polynucleotides in response to medium pH and temperature. (C) 2003 Elsevier Science (USA). All rights reserved.
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Structure-designed Peptide Microarrays for Protein-Detection Reviewed
MIHARA Hisakazu, TAKAHASHI Mizuki, USUI Kenji, OJIMA Tetsunori, NOKIHARA Kiyoshi
2002 109 - 110 2003.2
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Polysaccharide-polynucleotide complexes part 17. Solvent effects on conformational-transition of polydeoxyadenylic acid in the complexes with schizophyllan
M Mizu, K Koumoto, T Kimura, K Sakurai, S Shinkai
POLYMER JOURNAL 35 ( 9 ) 714 - 720 2003
Publisher：SOC POLYMER SCIENCE JAPAN
This paper examines the relationship between the conformation and solvent for the poly(dA)/s-SPG complex using circular dichroism in the 240-300 nm wavelength region. At the low temperature and low salt concentration, poly(dA) in the complex is estimated to take C2'-endo with a different torsional angle from the anti form. With increasing temperature, the circular dichroism (CD) spectrum starts to bear characteristics of C3'-endo and anti as a minority among the majority of C2'-endo and anti. When the salt concentration is increased, the spectrum becomes almost identical to that of the high temperature form under the non-salt condition. Therefore, addition of salt provides the identical effect to increasing temperature. DO induces the same effect as decreasing temperature.
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アンチセンス治療に向けた多糖系遺伝子キャリアー
甲元一也、水雅美、櫻井和朗、新海征治
月刊BIOINDUSTRY 20 ( 10 ) 30 - 37 2003
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Covalent bond-mediated network fabrication of carboxylic anhydride-protected gold nanoparticles
K. Akamatsu, J. Hasegawa, H. Nawafune, H. Katayama and F. Ozawa
Trans. Mater. Res. Soc. Jpn. 28 927 - 930 2003
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低温鉛フリーはんだ接合を目的としたスズー亜鉛共晶合金の電折および亜鉛の共折機構
縄舟 秀美, 中谷 敏雄, 赤松 謙祐, 内田 衛, 小幡 恵吾
エレクトロニクス実装学会誌 6 ( 3 ) 222 - 227 2003
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ULSI銅微細配線の形成を目的とした中性無電解銅めっきにおけるビアフィリング特性の改善
縄舟秀美、樋口晋吾、赤松謙祐、内田衛
表面技術 54 ( 10 ) 683 - 688 2003
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Synthesis of metallized polymide films by surface chemical modification
AKAMATSU Kensuke
Trans. Mater. Res. Soc. Jpn. 28, 1267-1270 2003
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Synthesis of metallized polyimide films by surface chemical modification
S. Ikeda, K. Akamatsu and H. Nawafune
Trans. Mater. Res. Soc. Jpn. 28 1267 - 1270 2003
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Surface Modification-Based Synthesis and Microstructural Tuning of Nanocomposite Layers : Monodispersed Copper Nanoparticles in Polyimide Resins
Chem. Master. 15 ( 13 ) 2488 - 2491 2003
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Site-Selective Direct Silver Metallization on Surface-Modified Polyimide Layers
Langmuir 19 ( 24 ) 10366 - 10371 2003
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Ring-Opening methathesis Polymerization Using Alkenyl Sulfides as Chain-Transfer Agents: Efficient Routes to Unsymmetrical Poly(norbornen)-Based Macroinitiators Bearing a Terminal Hydroxy Group
Macromolecules 36 ( 19 ) 7020 - 7026 2003
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Preparation and characterization of Au.Co nano-alloys
J. Electroanal. Chem. 559 99 - 102 2003
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NH2-terminated poly(ethylene oxide) containing nano-sized NiO particles: synthesis, characterization and structural considerations
Chem. Mater. 15 ( 26 ) 4916 - 4922 2003
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Introdution of Ag and Ag2S nanoparticles into nylon 6 film and fiber
Polymer Journal 35 ( 12 ) 960 - 964 2003
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Fabrication of copper film on aluminum nitride by novel electroless plating
J. Electrochem. Soc. 150 ( 4 ) C260 - C265 2003
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Fabrication and structural characterization of nano composites consisting of Ni nanoparticles dispersed in polyimide films
Eur. Phys. J. D. 24 377 - 380 2003
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Covalent bond-mediated network fabrication of carboxylic anhydride-protected gold nanoparticles
AKAMATSU Kensuke
Trans. MRS-J 28 ( 3 ) 927 - 930 2003
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A new synthesis of functional dyes from 2-acenaphtho[1,2-c]pyrrole.
Heterocycles, 61, 433-447 2003
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Facile One-Pot Synthesis of Aromatic and Heteroaromatic Sulfonamides
J. Org. Chem. 68, 8274-8276 2003
DOI： 10.1021/jo034643j
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タンパク質に人工分子を結合させる新手法
中野修一・杉本直己
Bio Medical Quick Review Net 005 - 005 2003
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：メディカルドゥ
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Important effects of non-Watson-Crick interactions and cation binding on nucleic acid structures.
S. Nakano and N. Sugimoto
Recent research development in nucleosides & nucleotides 1 59 - 80 2003
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：Transworld Research Network
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'Pentacoordinated iron complexes with carboxamido nitrogens and thiolate sulfurs: As models for Fe-contoining nitrile hydratases
FUJII Satoshi
J. Inorg. Biochem. ( 96 ) 134 2003
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings)
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Microarrays with alpha-Helical Peptides for Protein Detection using a FRET Technique
K. Usui, M. Takahashi, A. Ueno, K. Nokihara, H. Mihara
Peptides 2002 646 - 647 2003
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Peptide microarrays using structure-based peptide libraries for protein chips
K Usui, M Takahashi, T Ojima, M Suzuki, K Nokihara, F Tamiya, H Mihara
BIOPOLYMERS 71 ( 3 ) 403 - 403 2003
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS INC
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プロテインチップ Reviewed
臼井 健二, 三原 久和
化学装置2003年4月号 36 - 38 2003
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Construction of Protein-Detection Microarrays with FRET Fingerprint Patterns Using an α-Helical Peptide Library. Reviewed
臼井健二, 高橋瑞稀, 軒原清史, 三原久和
生体機能関連化学シンポジウム講演要旨集 18th 2003
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Polysaccharide-polynucleotide interaction (XI); Novel separation system of RNAs by using Schizophyllan-appended column
T Kimura, K Koumoto, M Mizu, K Sakurai, S Shinkai
CHEMISTRY LETTERS ( 12 ) 1240 - 1241 2002.12
Publisher：CHEMICAL SOC JAPAN
Schizophyllan belongs to the beta-1,3-glucan family and can form a complex with particular polynucleotides.(1) We applied this complexation to specifically separate polynucleotides by constructing a schizophyllan-appended column. The column trapped poly(C) and poly(A), while it did not show any affinity to poly(G), which is consistent with the experimental results obtained in the homogenous solution. The present results indicate that the s-SPG column can be a new separation device for biomolecules.
DOI： 10.1246/cl.2002.1240
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ホヤRNAiプロトコル
藤原滋樹，西方敬人，菅沼一樹，佐藤愛，浦野亜規，片野美緒
細胞工学 21 771 - 777 2002.11
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：秀潤社
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「生命を化学する」・・・生命科学のすすめ 生命現象を化学に活用する
中野修一・杉本直己
化学 57 ( 11 ) 42 - 47 2002.11
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：化学同人
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Gel formation properties of a uracil-appended cholesterol gelator and cooperative effects of the complementary nucleobases
E Snip, K Koumoto, S Shinkai
TETRAHEDRON 58 ( 43 ) 8863 - 8873 2002.10
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
We designed and synthesized a uracil-appended cholesterol gelator (1) in order to control the gel stability and the gel morphology by addition of the complementary and non-complementary nucleobase derivatives. Compound 1 forms columnar stacks in cyclohexane due to the van der Waals interaction (cholesterol-cholesterol interaction) and the intergelator hydrogen bonding between uracil moieties. Addition of a 'monomeric' adenosine (3) into the gel only decreases the stability with increasing the concentration. The destabilization is ascribed to a lack of intergelator hydrogen bonding accompanied with forming the complementary base pairs between 1 and 3. In contrast, addition of adenine-appended cholesterol (7) induces a different behavior; with increasing 7 concentration the mixed gel is initially stabilized and then destabilized, giving rise to a maximum at the ratio of 1/7= 1:1 for the T-gel plot. One may consider, therefore, that when the additive has a common, column-forming cholesterol moiety, the cholesterol-cholesterol interaction can operate cooperatively with the complementary base pairing. In addition, the gel fiber structure is clearly changed by the addition of 7. Taking the fact that there is no report for such an additive effect inducing a structural change with maintaining the gel stability into consideration, our attempt combining cholesterol columnar stacks with the nucleobase additives provides a new methodology to control the stability and the morphology of organogels. (C) 2002 Elsevier Science Ltd. All rights reserved.
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Polysaccharide-polynucleotide complexes VIII. Cation-induced complex formation between polyuridylic acid and schizophyllan
K Sakurai, R Iguchi, K Koumoto, T Kimura, M Mizu, Y Hisaeda, S Shinkai
BIOPOLYMERS 65 ( 1 ) 1 - 9 2002.10
Publisher：JOHN WILEY & SONS INC
Schizophyllan belongs to the beta-1,3-D-glucan family, and dissolves as a single chain in dimethylsulfoxide (DMSO) and as a triple helix in water, respectively. It is already known that when we prepare a mixture of poly(C) and schizophyllan in DMSO and subsequently exchange the solvent to water, poly(C) anti schizophyllan can form a complex. When we applied this procedure to the poly(U) system, we could not induce the complex formation between poly(U) and schizophyllan at all. However, we found that addition of some alkaline cations is necessary to induce the complexation between poly(U) and schizophyllan. The complex stability strongly depends on both the cation species and the salt concentration. The complexation is sensitively reflected in the CD spectrum. The magnitude of the spectral change is followed by the order; Rb+ much greater than K+ > Na+ ? Cs+. This cation
DOI： 10.1002/bip.10189
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Chemical modification of curdlan: Characteristic complexation with polynucleotides by control of the molecular weight.
K Koumoto, K Sakurai, S Shinkai, T Kunitake
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 224 ( 2 ) U429 - U429 2002.8
Publishing type：Research paper, summary (international conference) Publisher：AMER CHEMICAL SOC
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Novel DNA-polysaccharide triple helix and its application to a gene carrier.
K Sakurai, S Shinkai
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 224 ( 2 ) U476 - U477 2002.8
Publishing type：Research paper, summary (international conference) Publisher：AMER CHEMICAL SOC
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「生命を化学する」・・・小分子で巨大タンパク質の挙動を操る タンパク質のドメイン・スワッピングとその制御
中野修一・杉本直己
化学 57 ( 8 ) 42 - 47 2002.8
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：化学同人
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Molecular design of "super" hydrogelators: Understanding the gelation process of azobenzene-based sugar derivatives in water
H Kobayashi, A Friggeri, K Koumoto, M Amaike, S Shinkai, DN Reinhoudt
ORGANIC LETTERS 4 ( 9 ) 1423 - 1426 2002.5
Publisher：AMER CHEMICAL SOC
[GRAPHIC]
As an attempt to rationally design aqueous organogelators, a bolaamphiphilic azobenzene derivative (1) bearing two sugar groups was synthesized. Compound 1 formed a gel in water even at concentrations as low as 0.05 wt% (0.65 mM). Spectroscopic studies and electron-micrographic observations have clarified the gel structure and the origin of the gelation ability for water.DOI： 10.1021/ol025519+
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アルツハイマー病とNO・金属イオンの化学
藤井敏司，杉本直己
化学 57 ( 4 ) 43 - 47 2002.3
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：化学同人
アルツハイマー病と一酸化窒素，金属イオンの関わりについて，総合的に論じた
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核酸の相互作用
杉本直己・中野修一
化学フロンティア5 生命化学のニューセントラルドグマ テーラーメイド・バイオケミストリーのめざすもの 24 - 34 2002.2
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：化学同人
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狂牛病・プリオン病と金属イオンの化学
藤井敏司，杉本直己
化学 57 ( 3 ) 30 - 34 2002.2
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：化学同人
狂牛病に代表されるプリオン病と金属イオンの関係について総合的に解説した
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Polysaccharide-polynucleotide complexes. Part 12. Enhanced affinity for various polynucleotide chains by site-specific chemical modification of schizophyllan
K Koumoto, T Kimura, M Mizu, T Kunitake, K Sakurai, S Shinkai
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 ( 22 ) 2477 - 2484 2002
Publisher：ROYAL SOC CHEMISTRY
Recently, we found that schizophyllan (SPG), which is a natural and neutral polysaccharide, can form macromolecular complexes with certain polynucleotides. In order to increase the stability and the affinity of the complexes, we synthesized a 2-aminoethanol-appended SPG (N-s-SPG) by utilizing periodate oxidation and reductive amination. Although the modi. cation level was only 2.4 +/- 0.3%, the melting temperature of the poly(C)-N-s-SPG complex was increased by 8 degreesC comparing with the poly(C)-s-SPG complex. In addition, the conformation of the complex was scarcely perturbed by the introduction of amino groups. When we formed complexes of N-s-SPG and other polynucleotides, the melting temperature of their complexes increased without exception. Moreover, the amino modification induced complexation with poly(U), which does not form a complex with SPG. The affinity for the short chain nucleotide was also enhanced. These results indicate that the present modi. cation method is quite useful to improve complex stability and we believe, therefore, that this strategy will make it possible to apply SPG to a novel gene carrier.
DOI： 10.1039/b207553a
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Sol-gel phase transition induced by fiber-vesicle structural changes in sugar-based bolaamphiphiles
H Kobayashi, K Koumoto, JH Jung, S Shinkai
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2 ( 11 ) 1930 - 1936 2002
Publisher：ROYAL SOC CHEMISTRY
The research objects of this paper were to design bolaamphiphilic gelators utilizing a sugar family as a source of solvophilic groups and an azobenzene segment as a solvophobic group and to monitor the aggregation mode utilizing the spectroscopic properties of the azobenzene chromophore. The results indicated that the bolaamphiphiles act, although only for specific DMSO-water mixtures, as gelators and form a unique supramolecular helical structure in the gel phase. The UV- Vis and CD spectra showed that the azobenzene segments adopt H- type face- to- face orientation and the dipole moments are arranged in the right- handed (R)- helicity. Since the fibrils as observed by electron microscope possess the right- handed helical structure, one may consider that the microscopic azobenzene-azobenzene orientation is reflected by the macroscopic supramolecular structure. When boronic acid- appended poly( L-lysine) was added, the gel phase was changed into the sol phase in the macroscopic level and the fibrous aggregate was changed into the vesicular aggregate in the microscopic level. These changes, which are usually induced by a temperature change, are due to the specific boronic acid- sugar interaction occurring at the constant temperature. Interestingly, when -fructose which shows high affinity with the boronic acid group was added, the sol phase and the vesicular aggregate were changed back to the gel phase and the fibrous aggregate, respectively. This means that the phase and morphological changes in the sugar- integrated bolaamphiphiles can be controlled reversibly.
DOI： 10.1039/b204631h
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Molecular Imprinting in Alcohols: utility of a pre-polymer based strategy for synthesizing stereoselective artificial receptor polymers in media hydrophilic
Anal.Chim.Acta 466, 11-15 2002
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ホヤのRNAiプロトコル
西方 敬人
細胞工学 21, 771-777 2002
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金ナノ粒子とバイオセンシング
赤松謙祐、杉本直己
化学 57 28 - 31 2002
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：化学同人
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LSIインターコネクションへの銅めっき法の適用
縄舟秀美、赤松謙祐
Electrochemistry 70 880 - 884 2002
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：a
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Effect of composition and structure of gold/copper bimetallic nanoparticles on dispersion in polymer thin films
J. Mater. Chem 12 ( 12 ) 3610 - 3614 2002
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金ナノ粒子とバイオセンシング
赤松 謙祐
化学 57 28 - 31 2002
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フリップチップ実装を目的としたスルホコハク酸浴からのスズ―インジウム共晶合金の電析
縄舟 秀美, 中谷 敏雄, 赤松 謙祐, 水本 省三, 小幡 恵吾, 内田 衛
エレクトロニクス実装学会誌 5 ( 4 ) 349 - 352 2002
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Novel copper electroless plating process on ceramic substrate using copper oxide
Electrochem.Solid State Lett. 5 ( 9 ) C87 - C89 2002
-
Microstructure and electron transport properties of AuxCo1-x nano-alloys embedded in polyacrylonitrile thin films
J.Master.Chem. 12 ( 8 ) 2408 - 2411 2002
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LSIインターコネクションへの銅めっき法の適用
赤松 謙祐
電気化学および工業物理化学 70 880 - 884 2002
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Highly reactive intermediate-functionalized gold clusters: Synthesis and immobilization on silica supports through amide-forming coupling
J.Master.Chem. 12 ( 10 ) 2862 - 2865 2002
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Growth of metal oxide thin films from aqueous solution by liquid phase depositiono, LPD, method
Solid State Ionics 151 ( 1/4 ) 1 - 9 2002
-
Fabrication and characterization of PAN-derived carbon thin films containing Au nanoparticles
Thin Solid Films 408 ( 1/2 ) 59 - 63 2002
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鉛フリーはんだ接合を目的としたスルホコハク酸浴からのSn-0.7Cu-0.3Ag３元合金の電析
縄舟秀美、芝一博、赤松謙祐、水本省三、内田衛、小幡恵吾
エレクトロニクス実装学会誌 5 ( 2 ) 146 - 151 2002
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Preparation of novel heteroisoindoles from nitro pyridines and nitro pyridones
Heterocycles 58, 301-310 2002
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Construction of a Protein-Detection System using Designed Peptides with Fluorescent Labels
K. Usui, M. Takahashi, A. Ueno, K. Nokihara, H. Mihara
Peptide Science 2001 405 - 406 2002
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核酸における超ワトソン・クリックモデルの試み カチオンと非ワトソン・クリック型塩基の役割を考える
杉本直己・中野修一
化学と生物 39 ( 11 ) 714 - 722 2001.11
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：日本農芸化学会
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Chemical modification of curdlan to induce an interaction with poly(C)(1)
K Koumoto, T Kimura, H Kobayashi, K Sakurai, S Shinkai
CHEMISTRY LETTERS ( 9 ) 908 - 909 2001.9
Publisher：CHEMICAL SOC JAPAN
The molecular weight of the commercially available curdlan is over 10(6), which makes it difficult to bind to polynucleotides. This paper presents a chain-scission reaction of curdlan and the molecular weight dependence of the binding ability. We found that curdlan can bind to poly(C) when the molecular weight is less than about 10(5). However, the complex stability is still less than that of the single chain of schizophyllan.
DOI： 10.1246/cl.2001.908
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Organization of nucleosides supported by boronic-acid-appended poly(L-lysine): Creation of a novel RNA mimic
H Kobayashi, M Amaike, K Koumoto, S Shinkai
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 74 ( 7 ) 1311 - 1317 2001.7
Publisher：CHEMICAL SOC JAPAN
Boronic-acid-appended poly(L-lysine) (1) can form anionic boronate esters with diol derivatives in aqueous solution. One can thus expect that when nucleosides are mixed with 1, the resultant complexes will satisfy the basic structural characteristics of RNA, viz., one-dimensionally organized nucleic bases and anionic charges. To obtain insights into this novel RNA mimic, the solution properties of adenosine (Ad) were estimated in the absence and the presence of 1. The large absorption and CD spectral changes were observed at pH 6-10 where the poly(L-lysine) main chain tends to adopt an a-helix-rich conformation. On the basis of the fact that the magnitude of the spectral changes is much larger than the fraction of complexed Ad molecules, the polymer-supported organization mode was proposed: that is, Ad molecules complexed by covalent bonds with I act as clusters to induce further organization of uncomplexed Ad molecules by noncovalent interactions. The proposal was further confirmed by H-1 NMR spectroscopy and dynamic light scattering, On the other hand, none of these spectral changes were observed for 2 ' -deoxyadenosine. The I-Ad complex thus formed can interact with complementary poly(U) but not with noncomplementary poly(C), indicating that it can act as a novel RNA mimic.
DOI： 10.1246/bcsj.74.1311
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Polysaccharide-polynucleotide complexes (IV): Antihydrolysis effect of the schizophyllan/poly(C) complex and the complex dissociation induced by amines
K Koumoto, T Kimura, K Sakurai, S Shinkai
BIOORGANIC CHEMISTRY 29 ( 3 ) 178 - 185 2001.6
Publisher：ACADEMIC PRESS INC
Polymer complexes formed by schizophyllan and poly(C) showed a unique antihydrolysis effect when poly(C) was subjected to hydrolysis under basic conditions. The complexation reduced the hydrolysis rate to 80% of the control (i.e., poly(C) itself). However, when we added oligoamines with the intention of catalyzing the hydrolysis, the oligoamines induced dissociation of the complex instead of acceleration of the hydrolysis. (C) 2001 Academic Press.
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TEM and SEM observations of super-structures constructed in organogel systems from a combination of boronic-acid-appended bola-amphiphiles with chiral diols
K Koumoto, T Yamashita, T Kimura, R Luboradzki, S Shinkai
NANOTECHNOLOGY 12 ( 1 ) 25 - 31 2001.3
Publisher：IOP PUBLISHING LTD
Two bola-amphiphilic alpha, omega -diboronic acids separated by a (CH(2))(11) or (CH(2))(12) group were synthesized. Complexation with chiral diols readily gave new amphiphiles end-capped with the chiral substituents. Some of these acted as good gelators of organic solvents. Transmission electron microscope and scanning electron microscope observations established that a variety of super-structures are created in the organogels, depending on the solvents and the structure of the chiral end-cap groups. In most cases, the fibrous aggregates, the network structure which is the driving-force for gelation, showed a helical higher-order structure reflecting the chirality of the end-cap groups. The results indicate that the combinatorial approach utilizing boronic acid functions and diol compounds is useful in creating a variety of new super-structures in the gel phase.
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A novel technique to specifically separate RNAs by Schizophyllan
Taro Kimira, Kazuya Koumoto, Masami Mizu, Ritsuko Kobayashi, Kazuo Sakurai, Seiji Shinkai
Nucleic Acids Res. Supplement 1 283 - 284 2001
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Thermally induced conformational-transition of polydeoxyadenosine in the complex with schizophyllan and the base-length dependence of its stability
M Mizu, T Kimura, K Koumoto, K Sakurai, S Shinkai
CHEMICAL COMMUNICATIONS ( 5 ) 429 - 430 2001
Publisher：ROYAL SOC CHEMISTRY
A single chain of schizophyllan, one of the beta -1,3-glucan family, can form a stoichiometric complex with poly(dA) and the poly(dA)'s conformation and the complex stability strongly depends on the base length.
DOI： 10.1039/b009943k
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Chemical modification of schizophyllan by introduction of a cationic charge into the side chain which enhances the thermal stability of schizophyllan–poly(C) complexes
Kazuya Koumoto, Taro Kimura, Masami Mizu, Kazuo Sakurai, Seiji Shinkai
Chemical Communications 1 ( 19 ) 1962 - 1963 2001
Combining oxidation with periodate and reductive amination, the amino group is introduced only in the side chain of schizophyllan and the product can bind to poly(C) with a higher dissociation temperature than that of the unmodified schizophyllan and poly(C) complex. © 2001 Royal Society of Chemistry.
DOI： 10.1039/b105609n
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Application of schizophyllan as a novel gene carrier
Kazuo Sakurai, Taro Kimira, Kazuya Koumoto, Masami Mizu, Ritsuko Kobayashi, Seiji Shinkai
Nucleic Acids Res. Supplement 1 223 - 224 2001
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分子転写法による新素材の開発 –生体分子を超える分子認識材料開発を目指して-
甲元一也, 新海征治
未来材料 1 ( 5 ) 35 - 41 2001
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Combinatorial Molecular Imprinting for Formation of Atrazine Decomposing Polymers
Chem.Lett. 530-531 2001
DOI： 10.1246/cl.2001.530
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Molecularly Imprinted Polymers with Metalloporphyrin-Based Molecular Recognition Sites Coassembled with Methacrylic Acid
Anal.Chem. 73, 3869-3874 2001
DOI： 10.1021/ac010278p
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Composition dependence on dispersion of Au/Cu bimetallic nanoparticles in nylon 11 thin films
AKAMATSU Kensuke
Eur.Phys.J.D 16 305 - 308 2001
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Composition dependence on dispersion of Au/Cu bimetallic nanoparticles in nylon 11 thin films
K. Akamatsu, T. Kawamura, and S. Deki
Eur. Phys. J. D 16 305 - 308 2001
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Direct photochemical formation of Cu patterns on surface modified polyimide resin
J.Mater.Chem. 11 ( 12 ) 2919 - 2921 2001
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添加剤の予備吸着を利用する銅電析プロセスによるULSI微細配線の形成
縄舟秀美、粟野まいこ、赤松謙祐、水本省三、内田衛、細田勉
表面技術 53 ( 1 ) 59 - 64 2001
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ポリイミド樹脂の表面改質および熱圧着を利用する銅のDirect Metallization
池田慎吾、赤松謙祐、縄舟秀美、水本省三、清田優
エレクトロニクス実装学会誌 4 ( 7 ) 603 - 606 2001
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Synthesis of titanium carbide from a composite of TiO2 nanoparticles/methyl cellulose by carbothermal reduction
Mater.Res.Bull. 36 ( 13/14 ) 2263 - 2275 2001
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Synthesis and microstructure of metal oxide thin films containing metal nanoparticles by liquid phase deposition(LPD) method
AKAMATSU Kensuke
Eur.Phys.J.D 16 325 - 328 2001
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Synthesis and microstructure of metal oxide thin films containing metal nanoparticles by liquid phase deposition (LPD) method
S. Deki, Hin Yu Yu Ko, T. Fujita, K. Akamatsu, M. Mizuhata, and A. Kajinami
Eur. Phys. J. D 16 325 - 328 2001
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Synthesis and characterization of Cu2O nanoparticles dispersed in NH2-terminated poly(ethylene oxide)
J.Mater.Chem. 11 ( 9 ) 2387 - 2389 2001
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Single-Electron Tunneling Effects in Nylon 11 Thin Films Containing Nanoparticles
Minoru Fujii, Yoku Inoue, Shinji Hayashi, Kensuke Akamatsu, Shigehito Deki
Jpn.J.Appl.Phys. 40 ( 3B ) 1911 - 1914 2001
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Preparation of Cu2O nanoparticles dispersed in NH2-terminated polyethylene oxide matrix
H. Yanagimoto, K. Akamatsu, K. Goto, and S. Deki
Eur. Phys. J. D 16 313 - 316 2001
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Preparation of Cu2O nanoparticles dispersed in NH2-terminated polyethylene oxide matrix
AKAMATSU Kensuke
Eur.Phys.J.D 16 313 - 316 2001
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Preparation and characterization of metal nanoparticles dispersed in polyacrylonitrile thin film
Scripta Mater. 44 ( 8/9 ) 1879 - 1882 2001
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Novel fabrication method for Si1-xTixO2 thin films with graded composition profiles by liquid phase deposition
J. Mater. Chem. 11 ( 4 ) 984 - 986 2001
-
Formation process and characterization of copper oxide nanoparticles dispersed in polymer thin films
Scripta Mater. 44 ( 8/9 ) 2149 - 2152 2001
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Formation of copper nanoparticles in surface modified polymide resin
赤松 謙祐
Proc. of the 7th International Symposium on Advanced Physical Fields 392-395 2001
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Formation of copper nanoparticles in surface modified polyimide resin
K. Akamatsu, S. Ikeda, H. Nawafune, and S. Deki
Proc. of the 7th International Symposium on Advanced Physical Fields 2001 392 - 395 2001
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正しいコンピュータ解析・処理の仕方 Invited
藤井 敏司
第2回ESR基礎セミナー(主催 : 磁気共鳴医学会・SFRR/JP), 京都, 2001.7 2001
Publishing type：Lecture material (seminar, tutorial, course, lecture, etc.)
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Polysaccharide-polynucleotide complexes (III): A novel interaction between the beta-1,3-glucan family and the single-stranded RNA poly(C)
T Kimura, K Koumoto, K Sakurai, S Shinkai
CHEMISTRY LETTERS ( 11 ) 1242 - 1243 2000.11
Publisher：CHEMICAL SOC JAPAN
We measured circular dichroism spectra to examine the complex-formation capability of various kinds of polysaccharides with poly(C). The results show that schizophyllan and lentinan can interact with poly(C), on the other hand, amylose, pullulan, dextran, and curdlan cannot. This difference clearly indicates that the water soluble beta -1,3-glucan can bind with polynucleotides. The molecular weight dependence of the complex formation has established that at least 30 glucose sequence length is necessary to incorporate the most bases into the complex.
DOI： 10.1246/cl.2000.1242
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Colorimetric sugar sensing method useful in "neutral" aqueous media
K Koumoto, S Shinkai
CHEMISTRY LETTERS ( 8 ) 856 - 857 2000.8
Publisher：CHEMICAL SOC JAPAN
Azobenzene derivatives bearing one or two aminomethylphenylboronic acid groups were synthesized: they were confirmed to be useful for practical colorimetric saccharide sensing in "neutral" aqueous media.
DOI： 10.1246/cl.2000.856
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The first example of positive allosterism in an aqueous saccharide-binding system designed on a Ce(IV) bis(porphyrinate) double decker scaffold
A Sugasaki, M Ikeda, M Takeuchi, K Koumoto, S Shinkai
TETRAHEDRON 56 ( 27 ) 4717 - 4723 2000.6
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
The first example of a positive, homotropic system in aqueous saccharide-binding has been achieved using a Ce(IV) bis(porphyrinate) double decker scaffold bearing two pairs of boronic acid groups (compound 2). In this system, the binding of the first guest (1:2 saccharide/boronic acid complex) suppresses the rotational freedom of the two porphyrin planes, which facilitates the binding of the second guest. As a result, two pairs of boronic acid groups in 2 can autoacceleratively bind these guests and yield CD-active species. The analyses of CD intensity-guest concentration plots according to the Hill equation resulted in K=3.7x10(4) M-2 and n=2.0 for D-fructose and K=9.6x10(5) M-2 and n=1.6 for D-glucose. The present system which is in action even in aqueous media is widely applicable to allosteric control of drug release, catalytic reaction, information transduction, etc. of saccharide-containing guest molecules. (C) 2000 Elsevier Science Ltd. All rights reserved.
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Atrazine-Selective Polymers Prepared by Molecular Imprinting of Trialkylmelamines as Dummy Template Species of Atrazine(共著)
MATSUI Jun
Anal. Chem. 72 ( 8 ) 1810 - 1813 2000
-
Molecularly Imprinted Polymer as 9-Ethyladenine Receptor Having a Porphyrin-Based Recognition Center(共著)
MATSUI Jun
J. Am. Chem. Soc. 122 ( 21 ) 5218 - 5219 2000
-
Molecularly Imprinted Fluorescent-Shift Receptors Prepared with 2-(Trifluoromethyl) acrylic acid(共著)
MATSUI Jun
Anal. Chem. 72 ( 14 ) 3286 - 3290 2000
-
Au超微粒子を埋め込んだナイロン11蒸着膜の構造と物性
赤松謙祐、出来成人、井上翼、藤井稔、林真至
信学技報 OME 200-99 1 - 6 2000
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Formation of Co filled carbon nanocapsules by metal-template graphitization of diamond nanoparticles
J. Appl. Phys. 88 ( 9 ) 5452 - 5456 2000
-
Single-electron tunneling effects in thin nylon 11 thin films containing gold nanopartides
Thin Solid Films 372 ( 1/2 ) 169 - 172 2000
-
Preparation and structure of copper nanoparticles/poly(acrylic acid)composite films
J. Mater. Chem. 10 ( 11 ) 2548 - 2552 2000
-
Preparation and characterization of polymer thin films containing silver and silver sulfide nanopartides(共著)
Thin Solid Films 359 ( 1 ) 55 - 60 2000
-
In situ spectroscopic and microscopic study on dispersion of Ag nanoparticles in polymer thin films
J. Phys. Chem. B 104 ( 44 ) 10168 - 10173 2000
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核酸二重鎖の安定性予測とアンチセンス法への適用：（１）基礎編
川上純司、杉本直己
Antisense 4 3 - 16 2000
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核酸二重鎖の安定性予測とアンチセンス法への適用：（２）応用編
川上純司、杉本直己
Antisense 4 71 - 82 2000
-
Synthesis and coplanarity-dependent HOMO-LUMO separation of conjugated dimers
J. Chem. Soc., Perkin Trans.1 2671-2675 2000
-
Synthesis and X-ray structure of stable 2H-isoindoles
J. Chem. Soc., Perkin Trans.1 995-998 2000
DOI： 10.1039/a908641b
-
"Iron complex with dithiocarbamate derivatives as trapping agents for endogenously produced nitric oxide" Invited
FUJII Satoshi
6th International Symposium on Spin Trapping 5 2000
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings)
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Nitric oxide production in the rat brain during experimental meningitis : Protective effects of phenyl ┣DBN(/)-┫DB-tert-butyl nitrone(共著)
FUJII Satoshi
10th Biennial Meeting of the International Society for Free Radical Research, Kyoto 2000.10/16-20) P12-2 186 2000
Publishing type：Research paper, summary (international conference)
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In vivo evidence of transnitrosylation from paramagnetic dinitrosyl dithiolato iron complex to another iron complex(共著)
FUJII Satoshi
Nitric Oxide, Biology and Chemistry, 1st International Conference on Biology, Chemistry and Therapeutic Applications of Nitric Oxide, San Francisco(USA)2000.6/3-7) 4 ( 3 ) 278 - 279 2000
Publishing type：Research paper, summary (international conference)
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Maternal cytoplasmic factors for generation of unique cleavage patterns in animal embryos.
Nishida, H., Morokuma, J., and Nishikata, T.
Curr. Top. Dev. Biol. 34 1 - 37 1999.11
Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Academic Press
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Super-structures can be constructed in organogels from combination of a boronic-acid-appended bolaamphiphile with chiral diols
T Kimura, T Yamashita, K Koumoto, S Shinkai
TETRAHEDRON LETTERS 40 ( 36 ) 6631 - 6634 1999.9
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
A bolaamphiphile bearing two boronic acid groups at the two ends was synthesized. Complexation with chiral diols readily afforded new amphiphiles end-capped with the chiral substituents. Some of them acted as gelators of organic solvents. TEM and SEM observations established that a variety of super-structures are created in the organogels, depending on the solvents and the structure of the end-cap groups. (C) 1999 Elsevier Science Ltd. All rights reserved.
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Combinatorial Molecular Imprinting : An Approach to Synthetic Polymer Receptors(共著)
MATSUI Jun
Anal. Chem. 71 ( 2 ) 285 - 290 1999
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Maternal cytoplasmic factors for generation of unique cleavage patterns in animal embryos.(共著)
NISHIKATA Takahito
Curr. Top. Dev. Biol. 34,1-37 1999
-
Novel preparation method of nano-sized gold particles by using NH22-terminated polyethylenoxide(共著)
J. Colloid Interface Sci. 214 ( 1 ) 123 - 125 1999
-
TEM investigation and electron diffraction study on dispersion of gold nonoparticles into nylon 11 thin film during heat treatment(共著)
J. Colloid Interface Sci. 214 ( 2 ) 353 - 361 1999
-
Synthesis and characterization of gold-pallaclium bimetallic partides dispersed in polymer thin film matrix(共著)
Nanostruct. Mater. 11 ( 1 ) 59 - 65 1999
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Preparation, characterization and optical properties of nano-sizod bimetallic particles dispersed in polymer thin films(共著)
AKAMATSU Kensuke
Trans. Mater. Res. Soc. of Jpn. 24,109-112 1999
-
Preparation, characterization and optical properties of nano-sized bimetallic particles dispersed in polymer thin films
K. Akamatsu, Y. Hatakenaka, M. Mizuhata, A. Kajinami, and S. Deki
Trans. Mater. Res. Soc. Jpn. 24 109 - 112 1999
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リボザイム（RNA酵素） − その工業的利用
杉本直己、川上純司
化学工業 50 204 - 210 1999
-
試験管内進化（in vitro evolution）
川上純司、杉本直己
日本ファジィ学会誌 11 779 1999
-
"Novel reductive nitrosylation realized in Fe((]G0003[))complexes having sulfur-containing Ligands"
FUJII Satoshi
J. Inorg. Biochem. 74 132 1999
Publishing type：Research paper, summary (international conference)
-
「生体内NOを捕捉する錯体」
藤井 敏司
第49回錯体化学討論会,ミニシンポジウム「今ニトロシル錯体が新しい」 1999
Publishing type：Research paper, summary (national, other academic conference)
-
「一酸化窒素の測定」
藤井 敏司
ESRセミナー(磁気共鳴医学会主催、SFRR Japan共催) 1999
Publishing type：Research paper, summary (national, other academic conference)
-
「一酸化窒素のin vivo測定」
藤井 敏司
ESRセミナー(磁気共鳴医学会主催、SFRR Japan共催) 1999
Publishing type：Research paper, summary (national, other academic conference)
-
"四塩化炭素肝障害にて発生した一酸化窒素のジチオカルボキシサルコシン鉄錯体による捕捉"
藤井 敏司
磁気共鳴と医学(日本医学館) 10 16 - 20 1999
Publishing type：Research paper, summary (national, other academic conference)
-
"内因性一酸化窒素のジチオカルバメート誘導体鉄錯体による捕捉検出〜検出法の基礎的検討〜"
藤井 敏司
磁気共鳴と医学(日本医学館) 10 21 - 25 1999
Publishing type：Research paper, summary (national, other academic conference)
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タンパク質の機能を担う低分子ペプチド 化学で生物活性を再現する
杉本直己・中野修一
化学 53 ( 6 ) 40 - 44 1998.6
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：化学同人
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Design of a visualized sugar sensing system utilizing a boronic acid azopyridine interaction
K Koumoto, M Takeuchi, S Shinkai
SUPRAMOLECULAR CHEMISTRY 9 ( 3 ) 203 - + 1998
Publisher：TAYLOR & FRANCIS LTD
The boronic acid-amine (B-N) interaction was applied to the molecular design of a visualized sensing system for saccharides. 3-Nitrophenyl-boronic acid (3b) interacts with the pyridine nitrogen of 4-(4-dimethylaminophenyl-azo) pyridine (2) in methanol and changes its color from yellow to orange. Added saccharides form complexes with 3b and enhance the acidity of the boronic acid group. As a result, the B-N interaction becomes stronger and the intensified intramolecular charge-transfer band changes the solution color to red. By combined (1)H-NMR and absorption spectroscopic studies, four association constants governing the present ternary complex system have been determined. In case of D-fructose, the 3b.D-fructose complex can interact with 2240 times more strongly than 3b and the 2.3b complex can associate D-fructose 241 times more strongly than 3b. Clearly, the B-N interaction not only enables the colorimetric saccharide sensing but also enhances the association ability (i.e., sensitivity). Thus, the present paper offers a novel basic concept for molecular design of visualized saccharide sensing systems.
-
Genes of the ascidian : An annotated list as of 1997. Reviewed
NISHIKATA Takahito
Zoological Science 15 ( 5 ) 625 - 643 1998
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
DOI： 10.2108/zsj.15.625
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Dispersion of gold nanoparticles into a nylon 11 thin film during heat treatment : in situ optical transmission study
Journal of Materials Chemistry 8 ( 3 ) 637 - 640 1998
-
Preparation and characterization of copper((]G0001[))oxide nanoparticles dispersed in polymer matrix(共著)
Journal of Materials Chemistry 8 ( 8 ) 1865 - 1868 1998
-
生命化学を担うニューバイオテクノロジー − ファージディスプレイ，インビトロセレクション，コンビナトリアルケミストリー
杉本直己、川上純司
化学 53 74 - 75 1998
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"Reaction Mechanism of Nitric Oxide with a Nitric Oxide Trapping Agent, Iron((]G0003[))-N-(dithiocarboxy)sarcosine Complex"
FUJII Satoshi
Nitric Oxide(Arch. Biochem. Biophys. Part B. ) 2 99 1998
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings)
-
Characterization and optical properties of gold nanoparticles dispersed in nylon 11 thin films
Journal of Materials Chemistry 7 ( 9 ) 1773 - 1777 1997
-
Nanoscale metal particles dispersed in polymer matrix
Nanostructured Materials 8 ( 8 ) 1121 - 1129 1997
-
Sequence diversity of an RNA library that interacts with Tat of HIV-1 in the presence of Zn2+ ion
J. Kawakami and N. Sugimoto
Abst. PRE-ICBIC-8 Okazaki Symp 46 1997
-
"Electron Paramagnetic Resonance Detection and Imaging of Endogenously Synthesized and Exogenously Supplied Nitric Oxide"
FUJII Satoshi
J. Magn. Reson. Anal. 3 125 - 140 1997
-
"Electron Paramagnetic Resonance Detection of Nitric Oxide in the Brain During Sepsis and Meningitis"
FUJII Satoshi
2nd International Conference on Bioradicals(ICB(]G0002[)) 28 1997
-
"In vivo EPR Detection and Imaging of Nitric Oxide Production from a Nitrovasodilator, Isosorbide Dinitrate"
FUJII Satoshi
J. Inorg. Biochem. 67 299 1997
-
「若手研究者によるNO研究」(座談会形式記事)
藤井 敏司
日本薬学会誌「ファルマシア」 33 473 - 478 1997
-
「元素111の新知識」
藤井 敏司
講談社ブルーバックス 247 - 254 1997
-
"Three-Dimensional In Vivo EPR Imaging of NO Production from a Nitrovasodilator, Isosorbide Dinitrate"
FUJII Satoshi
Jpn. J. Pharmacol. 75 17 1997
-
"The origin of an EPR signal observed in dithiocarbamate-loaded tissues : Copper((]G0002[))-dithiocarbamate complexes account for the narrow hyperfine lines"
FUJII Satoshi
Biochim. Biophys. Acta 1335 242 - 245 1997
-
"Synthesis of Iron((]G0003[))Benzenedithiolate Complexes Having Intramolecular NH--S Hydrogen Bond and NO-Derivatives"
FUJII Satoshi
J. Inorg. Biochem. 67 297 1997
-
"Iron Dithiocarbamate Complex as an NO Trapping Agent. In vivo EPR Detection and Imaging of Nitric Oxide Production from a Nitrovasodilator, Isosorbide Dinitrate"
FUJII Satoshi
Pre ICBIC 8 OKAZAKI Symposium 1997
-
"In vivo Three-Dimensional EPR Image of Nitric Oxide Production from a Nitrovasodilator, Isosorbide Dinitrate in Mouse Upper Abdomen"
FUJII Satoshi
2nd International Conference on Bioradicals(ICB(]G0002[)) 29 1997
-
"In Vivo ESR-CT Imaging of the Liver in Mice Receiving Subcutaneous Injection of Nitric Oxide-Bound Iron Complex"
FUJII Satoshi
Magn. Reson. Imag. 15 249 - 253 1997
-
"In vivo EPR spectroscopy of endogenously produced NO in the brain of septic-shock model rat"
FUJII Satoshi
Jpn. J. Pharmacol. 75 48 1997
-
初期発生における不等分裂のしくみ
西方敬人、西田宏記
細胞工学 15 ( 12 ) 1687 - 1697 1996.11
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：秀潤社
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Efficient glucoside extraction mediated by a boronic acid with an intramolecular quaternary ammonium ion
M Takeuchi, K Koumoto, M Goto, S Shinkai
TETRAHEDRON 52 ( 40 ) 12931 - 12940 1996.9
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
To develop an efficient sugar extractant on the basis of the mechanistic view a phenylboronic acid bearing a trioctylammonium group at the ortho position (4) was synthesized. To avoid the complexity we employed a simple two-phase solvent-extraction system which corresponds to the first step in membrane transport, i.e., extraction from a donating aqueous phase to an organic liquid membrane phase. The extraction rates and equilibria were estimated using alpha-p-nitrophenyl-D-glucopyranoside as a sugar and 1,2-dichloroethane as an organic phase and compared with those of a 2-methylphenylboronic acid (5)/trioctylmethylammonium chloride (TOMAC) 1 : 1 binary system. The extraction rates for 4 were faster by 2.5 - 29 fold than those for 5 + TOMAC. The distribution coefficients were also enhanced by 5 - 8 fold. The results indicate that the intramolecular quaternary ammonium group is very effective to neutralize the anionic charge developed in the boron atom upon sugar-binding and create extractable zwitterionic sugar complexes. Copyright (C) 1996 Elsevier Science Ltd
-
The mechanisms of unequal divisions in early development.
NISHIKATA Takahito
Cell Technology 15 ( 12 ) 1687 - 1697 1996
-
Preparation and characterization of Au-dispersed TiO2 thin films
Journal of Materials Chemistry 6 ( 12 ) 1879 - 1882 1996
-
リボザイム医薬の開発状況と今後の課題
杉本直己、川上純司
医薬ジャーナル 32 2489 - 2495 1996
-
"In vivo EPR detection and imaging of endogenous nitric oxide by using a 700 MHzEPR and DTCS-Fe complex as a spin-trapping reagent"
FUJII Satoshi
38th Rocky Mountain Conference, Denver(U. S. A. ) 100 1996
-
"化学の目でみたNOの正体"
藤井 敏司
別冊医学のあゆみNOのすべて(医歯薬出版株式会社) 3 - 7 1996
-
"内因性NOのin vivo ESR測定とその画像化"
藤井 敏司
別冊医学のあゆみNOのすべて(医歯薬出版株式会社) 30 - 34 1996
-
"ニトロシル鉄錯体をスピンプローブとしたL-band ESRイメージング"
藤井 敏司
磁気共鳴と医学(日本医学館) 7 169 - 172 1996
-
"Spectroscopic characterization of nitrosylheme in nitric oxide complexes of ferric and ferrous cytochrome c' from photosynthetic bacteria"
FUJII Satoshi
Biochim. Biophys. Acta 1292 39 - 46 1996
-
"NOと非ヘム鉄タンパク質"
藤井 敏司
季刊 化学総説「NO-化学と生物」(学会出版センター) 116 - 125 1996
-
"Nitric Oxide Trapping Efficiencies of Water-Soluble Iron((]G0003[))Complexes with Dithiocarbamate Derivatives"
Satoshi Fujii, Tetsuhiko Yoshimura, Hitoshi Kamada
Chem. Lett. ( 9 ) 785 - 786 1996
-
"Nitric Oxide Release from Porcine Aorta Endothelial Cells Measured by Electron Paramagnetic Resonance Spectroscopy"
FUJII Satoshi
Chem. Lett. ( 12 ) 1055 - 1056 1996
-
"In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice"
FUJII Satoshi
Nature Biotechnology 14 ( 8 ) 992 - 994 1996
-
リボザイムの構造と分子設計
大塚栄子、川上純司
蛋白質 核酸 酵素 40 1400 - 1407 1995
-
核酸の機能と構造に関する微視的相互作用 XI –DNAの一本鎖部位の安定性とペプチド認識における役割
杉本直己・中野修一・米山麻里・佐々木宗夫
甲南大学紀要理学部編 42 37 - 41 1995
Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：甲南大学
-
"Electron Paramagnetic Resonance Studies of Ferric Cytochrome c' from Photosynthetic Bacteria"
FUJII Satoshi
Biochim. Biophys. Acta 1251 161 - 169 1995
-
"EPR Study on[N-(dithiocarboxy)sarcosine]iron((]G0002[))Complexes with exogenous and endogenous NO"
FUJII Satoshi
J. Inorg. Biochem. 59 225 1995
-
"ESR Studies of Cytochrome c' from Photosynthetic Bacteria : Anomolous Electronic Ground State"
FUJII Satoshi
Magnetic Resonance in Medicine 6 225 - 227 1995
-
"Photosynthetic Bacterial Cytochrome c' : Correlation between Electronic Ground State and Reactivity toward Nitric Oxide"
FUJII Satoshi
J. Inorg. Biochem. 59 458 1995
-
"Nitrosyl Complexes of Cytochrome c' from Photosynthetic Bacteria : Spectroscopic Characterization of Heme Environments"
FUJII Satoshi
Magnetic Resonance in Medicine 6 222 - 224 1995
-
"In Vivo Electron Paramagnetic Resonance Imaging of NO-Bound Iron Complex in a Rat Head"
Tetsuhiko Yoshimura, Satoshi Fujii, Hidekatsu Yokoyama, Hitoshi Kamada
Chem. Lett. ( 4 ) 309 - 310 1995
-
RNA編集
川上純司、多比良和誠
BIO Clinica 9 525 - 531 1994
-
"Electron Paramagnetic Resonance Studies of Ferric Cytochrome c' from Photosynthetic Bacteria : Unique Electronic Ground State"
FUJII Satoshi
European Bioinorganic Chemistry Conference(]G0002[)(EUROBIC(]G0002[)) 133 1994
-
"Formation and Characteristics of Five Coordinate(Coordinately Unsaturated)Superoxo-Cobalt((]G0003[))Complexes"
FUJII Satoshi
J. Inorg. Biochem. 51 321 1993
-
"Model Studies of Catechol Dioxygenases. Important Role of Monodentate Catecholate-Iron((]G0003[))Intermediate"
Satoshi Fujii, Hiroaki Ohya-Nishiguchi, Noboru Hirota, Akira Nishinaga
Bull. Chem. Soc. Jpn. 66 ( 5 ) 1408 - 1419 1993
-
アンチセンスRNA・DNA - Part I -
川上純司、西川諭、多比良和誠
Pharm Tech Japan 8 247 - 264 1992
-
アンチセンスRNA・DNA - Part II -
川上純司、嶋山隆、大川淳、湯山則子、安田香央里、内丸忠文、上林正巳、西川富美子、西川諭、多比良和誠
Pharm Tech Japan 8 395 - 411 1992
-
RNA酵素の改良と発展性
川上純司、西川諭
化学と生物 30 244 - 248 1992
-
アンチセンスRNA・DNA - Part III -
大川淳、川上純司、湯山則子、安田香央里、嶋山隆、西川富美子、井口義夫、武部豊、西川諭、多比良和誠
Pharm Tech Japan 8 491 - 512 1992
-
"Characterization of neutrophil b-type cytochrome in situ by electron paramagnetic resonance spectroscopy"
FUJII Satoshi
FEBS. Lett. 281 ( 1/2 ) 130 - 132 1991
-
"DMPO Spin Trapping in the Presence of Fe Ion"
FUJII Satoshi
Free Rad. Res. Comms. 9 233 - 240 1990
-
"EPR Evidence of intermediate peroxo complexes formed in a SOD model system"
FUJII Satoshi
Inorg. Chim. Acta 175 ( 1 ) 27 - 30 1990
-
"DMPOの鉄イオンと錯体形成"
藤井 敏司
磁気共鳴と医学(日本医学館) 1 96 - 100 1990
-
"EPR Studies on the Structure and Reactions of Non-heme Iron, Substrate, and Dioxygen Ternary Complexes"
FUJII Satoshi
2nd Japan-China Bilateral ESR Symposium(第28回ESR討論会) 56 - 57 1989
-
細胞系譜を決める細胞質因子=細胞質による遺伝子発現のスイッチ(2)=
西方 敬人
遺伝 42 ( 4 ) 1988
-
Cytoplasmic determinants which determine the cell lineage. =Switching of the gene expression by the cytoplasmic determinants(2)=
NISHIKATA Takahito
The Heredity 42 ( 4 ) 1988
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)
-
ホヤ胚における筋肉細胞の分化機構
西方 敬人
遺伝 38 ( 7 ) 1984
-
The mechanisms of the muscle cell differentiation in the ascidian embryo.
NISHIKATA Takahito
The Heredity 38 ( 7 ) 1984