Review Papers (Misc) -
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Low M-W sulfated curdlan with improved water solubility forms macromolecular complexes with polycytidylic acid
K Koumoto, M Umeda, M Numata, T Matsumoto, K Sakurai, T Kunitake, S Shinkai
CARBOHYDRATE RESEARCH 339 ( 1 ) 161 - + 2004.1
Publisher：ELSEVIER SCI LTD
The water solubility of curdlan was enhanced by partial sulfation at O-6. Circular dichroism measurements suggest that the sulfated curdlan with the degree of substitution (DS) from 0 to 8.7 mol % forms macromolecular complexes with polycytidylic acid (poly(C)). Although the thermal stability of the complexes decreased with increase in DS, this could be overlapped by addition of NaCl in the concentration above that of serum. The results clearly indicate that the drawback arising from the electrostatic repulsion between the anionic charges can be partially compensated by the presence of salt. Furthermore, the polynucleotide chain complexed with the sulfated curdlan was protected from the enzymatic hydrolysis, corroborating the assumption that the sulfated curdlan has an ability to bind poly(C). (C) 2003 Elsevier Ltd. All rights reserved.
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Catalytic roles for proton transfer and protonation in ribozymes: Current understanding and future possibilities
P. C. Bevilacqua, T. S. Brown, S. Nakano, and R. Yajima
Biopolymers 73 ( 1 ) 90 - 109 2004.1
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：Wiley
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Enhancement of antisense effect by reducing interactions between proteins and ODNs
Masami Mizu, Kazuya Koumoto, Takahisa Anada, Ryouji Karinaga, Taro Kimura, Kazuo Sakurai, Takeshi Nagasaki, Seiji Shinkai
Bull. Chem. Soc. Jpn. 77 1101 - 1110 2004
DOI： 10.1246/bcsj.77.1101
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Schizophyllans carrying oligosaccharide appendages as potential candidates for cell-targeted antisense carrier
T Hasegawa, T Fujisawa, M Numata, T Matsumoto, M Umeda, R Karinaga, M Mizu, K Koumoto, T Kimura, S Okumura, K Sakurai, S Shinkai
ORGANIC & BIOMOLECULAR CHEMISTRY 2 ( 21 ) 3091 - 3098 2004
Publisher：ROYAL SOC CHEMISTRY
Schizophyllans carrying beta-lactoside and alpha-mannoside appendages were prepared from native schizophyllan through NaIO4 oxidation followed by reductive amination using aminoethyl-beta-lactoside and alpha-mannoside, respectively. The resulting schizophyllans form stable macromolecular complexes with polynucleotides, such as poly(C) and poly(dA). Specific affinity between these macromolecular complexes and saccharide-binding proteins was demonstrated by surface plasmon resonance and agarose gel staining assays. beta-Lactoside-appended schizophyllan enhanced an antisense activity in hepatocytes which express lactoside-binding proteins on their cell surfaces.
DOI： 10.1039/b412124b
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Polysaccharide/polynucleotide complexes. Part 6. Complementary-strand-induced release of single-stranded DNA bound in the schizophyllan complex
K Koumoto, M Mizu, K Sakurai, T Kunitake, S Shinkai
CHEMISTRY & BIODIVERSITY 1 ( 3 ) 520 - 529 2004
Publisher：VERLAG HELVETICA CHIMICA ACTA AG
Spectroscopic properties of single-stranded DNA/schizophyllan ternary complexes (ss-DNA - 2s-SPG), induced by addition of either complementary or noncomplementary strands, have been investigated. The addition of the complementary strands to ss-DNA - 2s-SPG induced the quick release of the bound ss-DNA to the complementary strands (both DNA and RNA), whereas the ternary complex was unaffected upon addition of noncomplementary strands. Our experiments imply that SPG has complexation properties indispensable to the gene carriers. As far as we know, there is no report on exploitation of such nonviral gene carriers that can accomplish an intelligent release of the bound ss-DNA toward the complementary strands. We believe, therefore, that SPG, a natural and neutral polysaccharide, has a great potential to become a new ss-DNA carrier.
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Molecular weight control of curdlan (beta-1,3-glucan polysaccharide) provides unique polynucleotide binding properties
K Koumoto, H Kobayashi, M Mizu, T Kimura, K Sakurai, T Kunitake, S Shinkai
POLYMER JOURNAL 36 ( 5 ) 380 - 385 2004
Publisher：SOC POLYMER SCIENCE JAPAN
Recently, we found that schizophyllan belonging to a beta-1,3-glucan polysaccharide family forms macromolecular complexes with certain single-stranded polynucleotides. The specific complex formation is originated from the beta-1,3-glucan skeleton in the main chain. In this paper, we have reported that curdlan, which is a beta-1,3-glucan polysaccharide without a side chain glucose unit, shows unique poly(C) binding properties different from those of schizophyllan; in the experiments using curdlan samples with the controlled molecular weight, the complexation ability appeared only in a limited "M-w range window". Stoichiometric analysis revealed that the "M-w range window" appears as a result of the competition between curdlan-curdlan self-association and curdlan-polynucleotide complexation. Furthermore, when the experiments were extended to other polynucleotides such as poly(A), poly(dA) and poly(dT), the "M-w range window" also appeared at their characteristic molecular weights. We believe, therefore, that curdlan can be applicable, in spite of its cheapness, as a precious functional material to gene technology.
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First observation by fluorescence polarization of complexation between mRNA and the natural polysaccharide schizophyllan
R Karinaga, M Mizu, K Koumoto, T Anada, S Shinkai, T Kimura, K Sakurai
CHEMISTRY & BIODIVERSITY 1 ( 4 ) 634 - 639 2004
Publisher：VERLAG HELVETICA CHIMICA ACTA AG
Schizophyllan is a natural beta-(1 --> 3)-D-glucan that exists as a triple helix in H2O and as a single chain in dimethylsulfoxide (DMSO) or basic solution (pH > 13). As we have already reported, when a homopolynucleotide (e.g., poly(dA), poly(A), or poly(C)) is added to a schizophylian/DMSO solution, and, subsequently, DMSO is exchanged for H2O, the single chain of schizophyllan forms a complex with the polynucleotide. Since eukaryotic mRNAs have poly(A) tails, we hypothesized that schizophyllan can bind to mRNA by interacting with this tail. However, we have not yet observed complexation between schizophyllan and mRNA after exchanging DMSO for H2O. In this report, we show that the complexation can be accelerated when the solution pH is changed from 13 to 7-8 in the presence of schizophyllan and polynucleotides. By this approach, we found that schizophyllan forms a complex with a yeast mRNA.
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Enhancement of the Antisense Effect of Polysaccharide-Polynucleotide Complexes by Preventing the Antisense Oligonucleotide from Binding to Proteins in the Culture Medium
Masami MIZU, Kazuya KOUMOTO, Takahisa ANADA, Ryouji KARINAGA, Taro KIMURA, Takeshi NAGASAKI, Seiji SHINKAI, Kazuo SAKURAI
Bull. Chem. Soc. Jpn. 77 ( 6 ) 1101 - 1110 2004
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多糖・核酸複合体を用いた機能性高分子コンジュゲート −核酸医薬デリバリーへの挑戦−
狩長亮二, 甲元一也, 櫻井和朗, 新海征治
高分子加工 53 561 - 568 2004
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Composite of Au Nanoparticles and molecularly imprinted polymer as a sensing material
SUGIMOTO N, AKAMATSU K, NISHIGUCHI S, MIYOSHI D, NAWAFUNE H, TAMAKI K, MATSUI J
Anal. Chem. 76 ( 5 ) 1310 - 1315 2004
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銅ナノ粒子分散ポリイミド樹脂と銅微細配線のナノスケール接合
縄舟秀美、赤松謙祐、池田慎吾
マテリアルステージ 3 58 - 62 2004
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：技術情報協会
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Aqueous solution-based synthesis of rare earth-doped metal oxide thin films
Thin Solid Films 460 ( 1/2 ) 83 - 86 2004
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Direct Patterning of Copper on Plymide Using Ion Exchangeable Surfece Templates Generated by Site-Selective Surface Modification
J. Am. Chem. Soc. 126 ( 35 ) 10822 - 10823 2004
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Syntesis, Surface Modification and Multilayer Construction of Mixed Monolayer-Protected CdS Nanoparticles
Langmuir 20 ( 25 ) 11169 - 11174 2004
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Preparation of transparent alumina film and fiber from a composite of aluminum polynuclear complex/methy cellulose
Mater. Chem. Phys. 83 ( 1 ) 54 - 59 2004
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Polyaddition of 2,7-diethyl-9, 9-dioctryfluorene using regio- andstereoselective alkyne dimerization catalysts
Macromolecules 37 ( 1 ) 13 - 17 2004
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Foramtion and Growth of Copper Nanoparticles from Ion-Doped Precursor Polymide Layers
J. Phys. Chem. B. 108 ( 40 ) 15599 - 15607 2004
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Electroless Copper Plating Using Sn(Ⅱ) Complex as a Reducing Agent for ULSI Metallization
AKAMATSU Kensuke
Mem. Konan Univ., Sci. Eng. Ser. 51, 29-38 2004
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Electroless copper plating using Sn(II) complex as a reducing agent for ULSI metallization
H. Nawafune, Y. Kobata, K. Akamatsu, S. Mizumoto, Y. Murakami
Mem. Konan Univ., Sci. Eng.Ser. 51 29 - 38 2004
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銅ナノ粒子分散ポリイミド樹脂と銅微細配線のナノスケール接合
赤松 謙祐
マテリアルステージ 3, 58-672 2004