Review Papers (Misc) -
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Competition between polysaccharide/polynucleotide complexation vs., polynucleotide hybridization; salt concentration dependence of the reaction direction
Ryouji Karinaga, Kazuya Koumoto, Jusaku Minari, Masami Mizu, Takahisa Anada, Kazuo Sakurai, Seiji Shinkai
e-Journal of Surface Science and Nanotechnology 3 38 - 45 2005.2
Schizophyllan is a natural β-(1→3)-D-glucan existing as a triple helix in water and as a single chain in dimethylsulfoxide (DMSO), respectively. As we already reported, when a homo-polynucleotide [ex. poly (A) or poly(dA)] was added to the schizophyllan/DMSO solution and subsequently DMSO was exchanged for water, the single chain of schizophyllan formed a complex with the polynucleotide. In this paper, using an adenine-thymine (or uracil) double strand as a model system, we explored whether s-SPG can unzip the polynucleotide duplexes, examining the competition between the schizophyllan/polynucleotide complexation and the polynucleotide hybridization with circular dichroism and fluorescence spectroscopy at various NaCl concentrations. The unzipping reaction happened for the hetero-duplexes (i.e., RNA-DNA duplexes) at low salt concentrations, on the other hand, it was not observed for the homo-duplexes at all the salt concentrations. When we compared dissociation temperatures, the unzipping reaction of the homo-duplexes was expected at low salt concentrations. This discrepancy between the experimental results and the expectation, as well as the different behavior between the hetero- and homo- duplexes, can be ascribed to the difference in the activation energy of intermediate states. © 2005 The Surface Science Society of Japan.
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テーラーメード・リボスイッチ
川上純司
高分子 54 ( 2 ) 93 2005.2
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：高分子学会
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Poly(N-substituted-acrylamide)-branched schizophyllans are useful for selective recovery of homopolynucleotides through convenient and quick precipitation procedures
T Matsumoto, M Umeda, M Numata, T Hasegawa, K Sakurai, K Koumoto, S Shinkai
POLYMER JOURNAL 37 ( 3 ) 177 - 185 2005
Publisher：SOC POLYMER SCIENCE JAPAN
Schizophyllans carrying poly(N-substituted-acrylamide)-branches were synthesized from native schizophyllan through Ce(IV)-initiated radical homo- or co-polymerization of N-iso-propyl- and N-sec-butyl-acrylamide. A series of structural analyses including elemental analysis, gel permeation chromatography, H-1 NMR, and enzymatic degradation using β-1,3-glucanase revealed that schizophyllans (150kDa) bearing long poly (N-substituted-acrylamide)-branches (ca. 290 kDa) are obtained. These SPG-conjugates form macromolecular complexes with certain homopolynucleotides such as poly(C), poly(dA), and poly(A) and then efficiently co-precipitate them on heating. For example, schizophyllan carrying poly(N-iso-propylacrylamide)-branches can selectively recover poly(C) from a mixture of poly(C) and poly(U) by simple heating (36° C) followed by centrifugation. HPLC analysis of the recovered polynucleotide revealed the high poly(C)-selectivity (poly(C):poly(U) = 95:5). Similarly, schizophyllan carrying more hydrophobic poly(N-iso-propylacrylamide-co-N-sec-butylacrylamide) -branches precipitates at lower temperature (ca. 20° C) and is applicable to isolation of poly(A) that forms less stable macromolecular complexes with SPG and SPG-derivatives (the dissociation temperatures are around 30° C).
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Transferrin-appended β-(1→3)-D-glucan schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake
Takahisa Anada, Ryouji Karinaga, Masami Mizu, Kazuya Koumoto, Takahiro Matsumoto, Munenori Numata, Seiji Shinkai, Kazuo Sakurai
e-J. Surface Sci. Nanotech. 3 195 - 202 2005
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An SPR Sensor Chip for Sensitive Detection of Small Molecules Using Molecularly Imprinted Polymer with Embedded Gold Nanoparticle
SUGIMOTO Naoki, AKAMATSU Kensuke, HARA Noriaki, MIYOSHI Daisuke, NAWAFUNE Hidemi, TAMAKI Katsuyuki, MATSUI Jun
Anal. Chem. 77 ( 13 ) 4282 - 4285 2005
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ナノテクノロジーの表面技術への展開
縄舟秀美、赤松謙祐、池田慎吾
表面技術 56 730 - 737 2005
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：表面技術協会
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ナノサイズ微粒子を利用した微細配線技術
縄舟秀美、赤松謙祐
日本金属学会誌 69 179 - 189 2005
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：日本金属学会
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量子ドット（Q-dot）の基礎とナノバイオエンジニアリング
赤松謙祐、鶴岡孝章、縄舟秀美、杉本直己
細胞工学 24 60 - 65 2005
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：学研メディカル秀潤社
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微細配線形成における湿式めっき法のプロセス・イノベーション
縄舟秀美、赤松謙祐、池田慎吾
科学と工業 79 34 - 40 2005
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：大阪工研協会
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化学的表面処理によるポリイミド樹脂のダイレクト・メタラリゼーション
赤松謙祐、池田慎吾、縄舟秀美
日本接着学会誌 41 325 - 330 2005
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：日本接着学会
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金属ナノ粒子分散ハイブリッド薄膜の調製
赤松謙祐、池田慎吾、縄舟秀美
化学工業 56 40 - 46 2005
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：a
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光触媒反応を利用したダイレクトパターニング
赤松謙祐、池田慎吾、縄舟秀美
表面技術 56 665 - 669 2005
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：表面技術協会
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A novel fabrication technique for interacting ferromagnetic-metal nanoparticle systems:fine-tuning of particle dimeter and interparticle spacing
S. Tomita, K. Akamatsu, H. Shinkai, S. Ikeda, H. Nawafune, C. Mitsumata, T. Kashiwagi, M. Hagiwara
Mater. Res. Soc. Proceedings 853E I5.10.1-I5.10.6 2005
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Band gap engineering of CdTe nanocrystals through chemical surface modification
K. Akamatsu, T. Tsuruoka, H. Nawafune
J. Am. Chem. Soc. 127 ( 6 ) 1634 - 1635 2005
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Controlling interparticle spacing among metal nanoparticles through metal-catalyzed decomposition of surrounding polymer matrix
K. Akamatsu, H. Shinkai, S. Ikeda, S. Adachi, H. Nawafune, S. Tomita
J. Am. Chem. Soc. 127 ( 22 ) 7980 - 7981 2005
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Tuning magnetic interactions in ferromagnetic-metal nanoparticle systems
S. Tomita, K. Akamatsu, H. Shinkai, S. Ikeda, H. Nawafune, C. Mitsumata, T. Kashiwagi, and M, Hagiwara
Phys. Rev. B 71 180414(R)1-4 2005
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Synthesis and structural control of metal-containing polyimide nanocomposite
H. Shinkai, S. Ikeda, K. Akamatsu, H. Nawafune, S. Tomita
Trans. Mater. Res. Soc. Jpn. 30 1223 - 1226 2005
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Surface functionalization of mixed monolayer-protected semiconductor nanocrystals
T. Tsuruoka, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. 30 1227 - 1230 2005
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Stereocontrolled synthesis and optical properties of all-cis poly(phenylene vinylene)s (PPVs): A method for direct patterning of PPVs
H. Katayama, M. Nagao, T. Nishimura, Y. Matsui, K. Umeda, K. Akamatsu, T. Tsuruoka, H. Nawafune, F. Ozawa
J. Am. Chem. Soc. 127 ( 12 ) 4350 - 4353 2005
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Site-selective direct photochemical deposition of copper on glass substrates using TiO2 nanocrystals
K. Akamatsu, A. Kimura, H. Matsubara, S. Ikeda, H. Nawafune
Langmuir 21 ( 18 ) 8099 - 8102 2005