Review Papers (Misc) -
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種々の細胞系を用いた新規αヘリックスペプチドの細胞導入活性スクリーニング Reviewed
菊池卓哉, 臼井健二, 高橋剛, 三原久和
日本化学会バイオテクノロジー部会シンポジウム講演要旨集 12th 2009
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金属ナノ粒子／ポリマー複合体フォトニック結晶の作製
鶴岡孝章、熊崎祥太、縄舟秀美、赤松謙祐
ケミカルエンジニヤリング 54 941 - 946 2009
Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：化学工業社
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DNAはなぜ二重らせん構造になるのか？ —分子間相互作用と分子環境効果を考えるー
杉本直己・中野修一
現代化学 450 ( 9 ) 25 - 30 2008.9
Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：東京化学同人
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Hydration is an important factor to regulate thermodynamic stability of a DNA duplex under molecular crowding conditions
Kazuya Koumoto, Hirofumi Ochiai, Naoki Sugimoto
CHEMISTRY LETTERS 37 ( 8 ) 864 - 865 2008.8
Publisher：CHEMICAL SOC JAPAN
By using a DNA duplex. (5'-GTTACTATATGA-3'/5'-TCATATAGTAAC-3') under molecular crowding, conditions, it was revealed that hydration plays a significant role in the stability of the duplex, in contrast to viscosity and dielectric constant which have little effect.
DOI： 10.1246/cl.2008.864
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NanoBioNow(10)周辺の分子環境に応答するDNAナノスイッチの構築
狩俣寿枝, 三好大輔, 藤本健史, 甲元一也, WANG Zhong‐Ming, 杉本直己
日本化学会講演予稿集 88th ( 2 ) 823 2008.3
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Structural effect of synthetic zwitterionic cosolutes on the stability of DNA duplexes
Kazuya KouMoto, Hirofumi Ochiai, Naoki Sugimoto
TETRAHEDRON 64 ( 1 ) 168 - 174 2008.1
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
The molecular design of useful cosolutes for polymerase chain reaction (PCR), which is one of the most important techniques in molecular biology, plays a significant role in amplification of highly stable genome sequences because during PCR, strand dissociation sometimes fails due to high melting temperature. Here, we designed and synthesized eight new zwitterionic cosolutes derived from glycine betaine, a destabilizing reagent for GC-rich DNA duplexes, and systematically compared their ability to destabilize DNA duplexes and to amplify genome DNA by PCR. We found that introduction of n-butyl groups rather than methyl groups into the ammonium group reduced the melting temperature of DNA duplexes 11-fold more than what was observed for the scaffold cosolute, glycine betaine, and furthermore, the cosolute can amplify the stable genome sequence by PCR. (c) 2007 Elsevier Ltd. All rights reserved.
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Enhanced amplification of polymerase chain reaction by addition of cosolutes derived from a cellular compatible solute. International journal
Kazuya Koumoto, Hirofumi Ochiai, Naoki Sugimoto
Nucleic acids symposium series (2004) 52 ( 52 ) 257 - 8 2008
We designed and synthesized artificial compatible solutes which can not only decrease the melting temperature of DNA duplexes dependent of their chemical structures but also improve the amplification of highly stable genome DNA sequence.
DOI： 10.1093/nass/nrn130
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Preparation and Characterization of Au Nanoparticle/Polymer Composite Photonic Crystals
松井 淳
Trans. MRS-Japan 33 717 - 720 2008
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Control of the optical properties of CdTe nanocrystals by selective exchange of Te with thiolate: effect of organic ligands on the formation of core-shell structures
T. Tsuruoka, R. Takahashi, K. Akamatsu, and H. Nawafune
Phys. Chem. Chem. Phys. 10 ( 16 ) 2221 - 2226 2008
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Direct metallization of nickel on polymeric template patterns for fabrication of copper circuits on glass substrates
Y. Matsumura, Y. Enomoto, M. Sugiyama, K. Akamatsu, H. Nawafune
J. Mater. Chem. 18 ( 42 ) 5078 - 5082 2008
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Nanocomposite polymeric microspheres containing Ni nanoparticles with controlled microstructures
K. Akamatsu, S. Adachi, T. Tsuruoka, S. Ikeda, S. Tomita, and H. Nawafune
Chem. Mater. 20 ( 9 ) 3042 - 3047 2008
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Immobilization and assembly of mixed monolayer-protected Au nanoparticles on glass substrate via covalent bond interconnection
T. Tsuruoka, A. Fujimoto, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn. 33 157 - 160 2008
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Highly luminescent mono- and multilayers of immobilized CdTe nanocrystals: controlling optical properties through post chemical surface modification
T. Tsuruoka, R. Takahashi, T. Nakamura, M. Fujii, K. Akamatsu, and H. Nawafune
Chem. Commun. ( 14 ) 1641 - 1643 2008
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Formation of metal thin film nanocomposite layer from precursor polyimide containing metal complex
M. Matsumura, Y. Enomoto, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn. 33 1333 - 1336 2008
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Fabrication of conductive thin films on precursor polyimide via chemical reduction of nickel (II) complex
Y. Matsumura, Y. Enomoto, T. Jyo, K. Akamatsu, H. Nawafune
J. Surf, Finish, Soc. Jpn. 59 ( 8 ) 562 - 564 2008
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Cationic Porphyrin Induced a Telomeric DNA to G-Quadruplex Form in Water.
Bioinorg. Chem. Appl., 2008
DOI： 10.1155/2008/294756
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分子クラウディング環境下においてDNA二重鎖の安定性に及ぼす水和の影響
甲元一也, 落合洋文, 狩俣寿枝, 中野修一, 杉本直己
高分子学会予稿集(CD-ROM) 56 ( 2 Disk1 ) 2X15 2007.9
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Delivery of antisense oligonucleotides to nuclear telomere RNA by use of a complex between polysaccharide and polynucleotide
Jusaku Minari, Takanori Kubo, Hideki Ohba, Naohiko Shimada, Yoich Takeda, Ryouji Karinaga, Takahisa Anada, Kazuya Koumoto, Takeshi Kawazu, Takeshi Nagasaki, Seiji Shinkai, Kazuo Sakurai
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 80 ( 6 ) 1091 - 1098 2007.6
Publisher：CHEMICAL SOC JAPAN
Telomerase, which is highly activated in neoplastic cells, can be a target for antisense therapy, and for that purpose, antisense oligonucleotides (AS ODNs) have to be effectively delivered into cellular nucleus where the target telomerase is present. The present work shows a new strategy to deliver AS ODNs to nucleus by use of a novel complex made from a natural polysaccharide schizophyllan (SPG) and AS ODNs. Nuclear transport is strictly regulated by the nuclear pore size and the related proteins. If the molecular weight of SPG is decreased, the SPG/AS ODN complex should be easily transported, although the stability of the complex decreases with a decrease in the molecular weight. We optimized the molecular weight of SPG to be 25 K. Furthermore, we attached importin-beta (a nuclear transport protein) to the side chain of SPG by use of a streptavidin-biotin interaction. When this complex was added to Jurkat cells, the telomerase activity was more suppressed than the naked dose, indicating that the importin-P in the complex induced the nuclear transport of the complexed AS ODN and the AS ODN inhibited the telomerase. The present work shows a new methodology for nuclear anti-sense therapy that should be important in future anti-cancer therapies.
DOI： 10.1246/bcsj.80.1091
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Artificial G-wire switch with 2,2 '-bipyridine units responsive to divalent metal ions International journal
Daisuke Miyoshi, Hisae Karimata, Zhong-Ming Wang, Kazuya Koumoto, Naoki Sugimoto
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 129 ( 18 ) 5919 - 5925 2007.5
Publisher：AMER CHEMICAL SOC
Development of a guanine nanowire (G-wire) that is controllable and can be switched by external signals is important for the creation of molecular electronic technologies. Here, we constructed a G-wire in which the thymines of the main chain of d(G(4)T(4)G(4)) were replaced with 2,2'-bipyridine units, which have two aromatic rings that rotate arbitrarily upon coordination with metal ions. Circular dichroism of the DNA oligonucleotides with or without the 2,2'-bipyridine unit showed that divalent metal ions induce the bipyridine-containing oligonucleotide to switch from an antiparallel to a parallel G-quadruplex. Native polyacrylamide gel electrophoresis showed that the parallel-stranded G-quadruplex DNA had a high-order structure. Circular dichroism and native gel electrophoresis analyses suggested that adding Na(2)EDTA causes a reverse structural transition from a parallel-stranded high-order structure to an antiparallel G-quadruplex. Moreover, atomic force microscopy showed a long nanowire (similar to 200 nm) in the presence of Ni2+ but no significant image in the absence of Ni2+ or in the presence of both Ni2+ and Na(2)EDTA. These observations revealed that the parallel-stranded high-order structure is a G-wire containing numerous DNA oligonucleotide strands bound together via divalent metal ion-2,2'-bipyridine complexes. Finally, we found that alternating addition of Ni2+ and Na(2)EDTA can cycle the G-wire between the high-order and disorganized structures, with an average cycling efficiency of 0.95 (i.e., 5% loss per cycle). These results demonstrate that a DNA oligonucleotide incorporating the 2,2'-bipyridine unit acts as a G-wire switch that can be controlled by chemical input signals, namely, divalent metal ions.
DOI： 10.1021/ja068707u
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細胞膜を通るための必要最低限の疎水性とは？ペプチドを用いた検証
川上純司
高分子 56 ( 3 ) 153 2007.3
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：高分子学会