Review Papers (Misc) -
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Site-selective deposition of gold on glass substrates using monolayer-protected gold nanoparticles
K. Akamatsu, H. Terahata, J. Hasegawa, H. Nawafune
J. Surf. Finish. Soc. Jpn. 56 ( 7 ) 427 - 428 2005
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Selective Electroless Copper Deposition on Aluminum Nitride Substrate with Patterned Copper Seed Layer
Thin Solid Films 491 ( 1-2 ) 18 - 22 2005
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Fabrication and structural control of Fe/Ti oxide thin films with graded compositional profiles by liquid phase deposition
S. Deki, S. Iizuka, K. Akamatsu, M. Mizuhata and A. Kajinami
J. Am. Ceram. Soc. 88 ( 3 ) 731 - 736 2005
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Amino Acid Ester Salt Recognition by Ferrocene-based Ditopic Receptor Bearing Oligoethylene Glycol with Pendant Bipy Subunits: CV, UV-vis and ESR Studies.
Org. Biomol. Chem. 3, 4060-4064 2005
DOI： 10.1039/b511233h
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Synthesis of water-soluble porphyrin and the corresponding highly planar benzoporphyrin without meso-substituents.
Tetrahedron Lett., 46, 113-116 2005
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A protein detection system with designed peptides using anomalous reflection of gold
S Watanabe, K Usui, K Tomizaki, H Mihara, M Watanabe, K Kajikawa
BIOPOLYMERS 80 ( 4 ) 599 - 599 2005
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS INC
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Designed peptide microarrays for protein detection and characterization
H Mihara, K Usui, K Tomizaki, K Nokihara
BIOPOLYMERS 80 ( 4 ) 493 - 493 2005
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS INC
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alpha-helical peptide microarrays using a dry peptide array method
K Usui, K Tomizaki, K Nokihara, H Mihara
BIOPOLYMERS 80 ( 4 ) 599 - 599 2005
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS INC
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Protein-Detecting Microarrays: Current Accomplishments and Requirements
K.-Y. Tomizaki, K. Usui and H. Mihara
ChemBioChem 6 782 - 799 2005
Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：WILEY-VCH
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Construction of Protein-Detection System using anomalous reflection of gold Reviewed
渡辺晋也, 臼井健二, 富崎欣也, 渡辺光昭, 梶川浩太郎, 三原久和
日本化学会講演予稿集 85th ( 2 ) 2005
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量子ドット（Q-dot）の基礎とナノバイオエンジニアリング
赤松謙祐、鶴岡孝章、縄舟秀美、杉本直己
細胞工学 24 60 - 65 2005
Publishing type：Article, review, commentary, editorial, etc. (other) Publisher：学研メディカル秀潤社
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Removal of the side-chain glucose groups from schizophyllan improves the thermal stability of the polycytidylic acid complexes under the physiological conditions
K Koumoto, R Karinaga, M Mizu, T Anada, K Sakurai, T Kunitake, S Shinkai
BIOPOLYMERS 75 ( 5 ) 403 - 411 2004.12
Publisher：JOHN WILEY & SONS INC
Thermal stabilization of the complex between polycytidylic acid [poly(Q] and the modified schizophyllan (SPG) whose hydrophilic side-chain glucose groups are selectively removed utilizing mild Smith-degradation has been investigated. With the decrease in the side-chain glucose groups of schizophyllan, the complex with poly(C) can be considerably stabilized compared with unmodified SPG; for example, the T. value after the removal of the side-chain glucose groups from 33.3 (unmodified) to 1.0 is enhanced by 14degreesC In addition, the thermal stabilization effect is even operative under the physiological conditions ([NaCl] = 0.15 mol dm(-3)). This effect is exerted owing to the construction of the hydrophobic atmosphere around the complex. Although schizophyllan lost the side-chain glucose groups, it still kept the protection effect of the bound poly(C) chain against RNaseA-mediated hydrolysis as observed for unmodified schizophyllan. The assessment of the cytotoxicity for A375:human malignant melanoma, and HL60:human promyelocytic leukemia revealed that the modified schizophyllan scarcely increases the cytotoxicity. These results indicate that the present modification for schizophyllan is of great significance in a viewpoint to develop the practical gene carriers operative even under the physiological conditions. (C) 2004 Wiley Periodicals, Inc.
DOI： 10.1002/bip.20148
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Proposal of new modification technique for linear double-stranded DNAs using the polysaccharide schizopyllan
T Anada, H Matsunaga, R Karinaga, K Koumoto, M Mizu, K Nakano, S Shinkai, K Sakurai
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 14 ( 22 ) 5655 - 5659 2004.11
Publisher：PERGAMON-ELSEVIER SCIENCE LTD
A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA)(80) tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture, that is, quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation. (C) 2004 Elsevier Ltd. All rights reserved.
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A polysaccharide carrier for immunostimulatory CpG DNAs to enhance cytokine secretion
M Mizu, K Koumoto, T Anada, T Matsumoto, M Numata, S Shinkai, T Nagasaki, K Sakurai
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 126 ( 27 ) 8372 - 8373 2004.7
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Protection of polynucleotides against nuclease-mediated hydrolysis by complexation with schizophyllan
M Mizu, K Koumoto, T Kimura, K Sakurai, S Shinkai
BIOMATERIALS 25 ( 15 ) 3109 - 3116 2004.7
Publisher：ELSEVIER SCI LTD
Schizophyllan is a beta-(1-->3)-D-glucan existing as a triple helix in water and as a single chain in dimethylsulfoxide (DMSO), respectively. As we already reported, when some homo-polynucleotide (for example, poly(dA) or poly(C)) is added to the schizophyllan/DMSO solution and subsequently DMSO is exchanged for water, the single chain of schizophyllan (s-SPG) forms a complex with the polynucleotide. The present work demonstrates that the polynucleotide bound in the complex is more stable to nuclease-mediated hydrolysis than the polynucleotide itself (i.e., naked polynucleotide), using high-performance liquid chromatography and ultraviolet absorbance technique. A kinetic study for the hydrolysis clarified that the simple Michaelis-Menten relation is held and the maximum velocity for the complex is one-sixth as small as that of the naked polynucleotide. This low hydrolysis rate for the complex suggests that s-SPG is applicable to a carrier for antisense oligonucleotides. (C) 2003 Elsevier Ltd. All rights reserved.
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Antisense oligonucleotides bound in the polysaccharide complex and the enhanced antisense effect due to the low hydrolysis
M Mizu, K Koumoto, T Anada, K Sakurai, S Shinkai
BIOMATERIALS 25 ( 15 ) 3117 - 3123 2004.7
Publisher：ELSEVIER SCI LTD
Schizophyllan is a beta-(1--> 3)-D-glucan and can form a novel complex with some single-chains of DNAs. As the preceding paper revealed, the polynucleotide bound in the complex is more stable to nuclease-mediated hydrolysis than the polynucleotide itself (i.e., naked polynucleotide). This paper examined possibility to apply this complex to an antisense DNA carrier, using an in vitro (cellfree) transcription/translation assay. In this assay, we used a plasmid DNA coding a green fluorescence protein (GFP) and an antisense DNA designed to hybridize the ribosome-binding site in the GFP-coded mRNA. When the antisense DNA was administered as the complex, a lower GFP expression efficiency (or higher antisense effect) is observed over naked DNA. This is because the antisense DNA in the complex is protected from the attack of deoxyribonuclease. When exonuclease I, which specifically hydrolyzes single DNA chains, was present in the GEP assay system, the antisense effect was not changed for the complex while being weakened in the naked antisense DNA system. These results imply that the exonuclease I cannot hydrolyze the antisense DNA in the complex, while it can hydrolyze naked DNA to reduce its antisense effect. (C) 2003 Elsevier Ltd. All rights reserved.
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『T細胞におけるアダプター分子3BP2の機能解析』(平成15(2003)年度公開セミナー : 老研研究発表会(平成15年1月〜12月))
川内 敬子
日本医科大学老人病研究所紀要 10 2004.3
Publisher：日本医科大学
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Long-term expression with a cationic polymer derived from a natural polysaccharide: Schizophyllan
T Nagasaki, M Hojo, A Uno, T Satoh, K Koumoto, M Mizu, K Sakurai, S Shinkai
BIOCONJUGATE CHEMISTRY 15 ( 2 ) 249 - 259 2004.3
Publisher：AMER CHEMICAL SOC
Among the various synthetic gene carriers based on biomaterials, cationic polymers with polysaccharide backbones have long been studied as nonviral vectors due to their low immunogenicity and high water solubility. Schizophyllan, a beta-(1,3)-glucan, is one of the various polysaccharides that are clinically administered. Furthermore, its safety in the human body has already been confirmed. Various functional groups can be selectively introduced into the side chain, not into the main chain of schizophyllan. Therefore, we have synthesized various oligoamine conjugates from schizophyllan. It was confirmed that their in vitro transfection efficiencies are superior to that of polyethylenimine by adjusting the molecular weight and the degree of amination of cationic schizophyllan. While it was possible to reduce cytotoxicity by adjusting the amount of DNA complex per cell, as seen with poly-L-lysine, polyethylenimine, and chitosan, PEGylation was the most effective means of reducing toxicity. Furthermore, using cationic schizophyllan carriers, it was also possible to express a reporter protein for a long period of time due to a long residence time of plasmid DNA in cells.
DOI： 10.1021/bc034178x
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Lactose-appended schizophyllan is a potential candidate as a hepatocyte-targeted antisense carrier
T Hasegawa, M Umeda, T Matsumoto, M Numata, M Mizu, K Koumoto, K Sakurai, S Shinkai
CHEMICAL COMMUNICATIONS ( 4 ) 382 - 383 2004.2
Publisher：ROYAL SOC CHEMISTRY
A schizophyllan (beta-1,3-glucan) derivative carrying lactose-appendages prepared by reductive amination can form stable macromolecular complexes with polynucleotides, shows excellent affinity with a lactose-binding lectin, and effectively mediates gene transfection into hepatocytes.
DOI： 10.1039/b313426a
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Chemically modified polysaccharide schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake efficiency
T Matsumoto, M Numata, T Anada, M Mizu, K Koumoto, K Sakurai, T Nagasaki, S Shinkai
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1670 ( 2 ) 91 - 104 2004.1
Publisher：ELSEVIER SCIENCE BV
Schizophyllan is a natural beta-(1 --> 3)-D-glucan existing as a triple helix in water and as a single chain in dimethylsulfoxide (DMSO), respectively. As we already reported, when some homo-phosphodiester polynucleotide (for example, poly(dA) or poly(C)) is added to the schizophyllan/DMSO solution and subsequently DMSO is exchanged for water, the single chain of schizophyllan forms a complex with the polynucleotide. Furthermore, we have already demonstrated that one of the potential applications of this novel complex is an antisense-oligonucleotide (AS ODN) carrier. This work describes a versatile and universal modification technique which enables us to introduce various functional groups only to the side chain of schizophyllan. This technique consists of periodate oxidation of the glucose side chain (it does not react with the main chain because of the absence of the 1,2-diol group in beta-(1 --> 3)-glucan) and subsequent introduction of the functional groups into the formyl terminate. In the present work, the introduced functional groups were spermine, octa-arginine (R8), arginine-glycine-aspartic acid tripeptide (RGD) and some amino or alpha-amino acid compounds. Using these compounds, we made the complexes and carried out an in vitro antisense assay for them, administrating a phosphorothioate AS ODN to the melanoma A375 or leukemia HL-60 cell lines to depress their c-myb mRNA. When we used the R8 or RGD modified schizophyllan as the antisense carrier, the antisense effect was most enhanced among others. Their superiority can be ascribed to enhancement of endocytosis due to these functional peptides. Furthermore, the cytotoxicity for these two modified schizophyllans was negligibly as small as the natural (unmodified) schizophyllan. One of the peculiar features of our system is that the complex (i.e., carrier + AS ODN) is charged negatively in total, which is different from the conventional systems. The present work has thus clarified that schizophyllan can act as a new potential candidate for AS ODN carriers. (C) 2004 Elsevier B.V. All rights reserved.