Review Papers (Misc) -
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半導体ナノ粒子の合成と発光特性制御
赤松謙祐、鶴岡孝章、縄舟秀美
科学と工業 80 315 - 321 2006
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：大阪工研協会
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A DNA duplex with extremely enhanced thermal stability based on controlled immobilization on gold nanoparticles
K. Akamatsu, M. Kimura, Y. Shibata, S. Nakano, D. Miyoshi, H. Nawafune, N. Sugimoto
Nano Lett. 6 ( 3 ) 491 - 495 2006
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Fabrication of fluorescent multilayers consisting of CdTe nanocrystals
T. Tsuruoka, R. Takahashi, K. Akamatsu, H. Nawafune
Trans. Mater. Res. Soc. Jpn. 31 425 - 428 2006
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Site-selective integration of monolayer-protected inorganic nanoparticles onto surface monolayer templates by solvent-induced lift-off process
K. Akamatsu, S. Samitsu, T. Tsuruoka, J. Hasegawa, H. Nawafune
Small 2 1130 - 1133 2006
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金属イオンレドックス系無電解めっき浴の安定性
赤松謙祐、増田昭仁、尾山祐斗、縄舟秀美
表面技術 57 ( 11 ) 799 - 801 2006
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A peptide array with a functional linker toward a cell assay chip format
T. Kakiyama, K. Usui, K. Tomizaki, H. Mihara
JOURNAL OF PEPTIDE SCIENCE 12 236 - 236 2006
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS LTD
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Detection of Interactions Between the Peptides Containing Nucleobase Amino Acids and Proteins using Anomalous Reflection of Gold Reviewed
渡辺晋也, 臼井健二, 富崎欣也, 梶川浩太郎, 三原久和
日本化学会講演予稿集 86th ( 2 ) 2006
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Construction of a screening system for peptide ligands controlling calcineurin activity using designed α-helical peptide arrays Reviewed
臼井健二, 富崎欣也, 三原久和
日本化学会講演予稿集 86th ( 2 ) 2006
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設計ペプチドを用いるアレイフォーマット開発とタンパク機能解析 Reviewed
臼井健二, 富崎欣也, 渡辺晋也, 三原久和, 梶川浩太郎
高分子学会予稿集(CD-ROM) 55 ( 2 Disk1 ) 2006
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半導体ナノ粒子の合成と発光特性制御
赤松謙祐、鶴岡孝章、縄舟秀美
科学と工業 80 315 - 321 2006
Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：a
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Linear double-stranded DNA that mimics an infective tail of virus genome to enhance transfection
T Anada, R Karinaga, K Koumoto, M Mizu, T Nagasaki, Y Kato, K Taira, S Shinkai, K Sakurai
JOURNAL OF CONTROLLED RELEASE 108 ( 2-3 ) 529 - 539 2005.11
Publisher：ELSEVIER SCIENCE BV
Our previous work showed that a natural beta-(1 -> 3)-D-glucan schizophyllan (SPG) can form a stable complex with single-stranded oligonucleotides (ssODNs). When protein transduction peptides were attached to SPG and this modified SPG was complexed with ssODNs, the resultant complex could induce cellular transfection of the bound ODNs, without producing serious cytotoxicity. However, no technique was available to transfect double-stranded DNAs (dsDNA) or plasmid DNA using SPG. This paper presents a new approach to transfect dsDNA, showing preparation and transfection efficiency for a minimal-size gene having a loop-shaped poly(dA)(80) on both ends. This poly(dA) loops of dsDNA can form a complex with SPG. An siRNA-coding dsDNA with the poly(dA) loop was complexed with Tat-attached SPG to silence luciferase expression. When LTR-Luc-HeLa cells that can express luciferase under the control of the LTR promoter were exposed to this complex, the expression of luciferase was suppressed (i.e., RNAi effect was enhanced). Cytotoxicity studies showed that the Tat-SPG complex induced much less cell death compared to polyethylenimine, indicating that the proposed method caused less harm than the conventional method. The Tat-SPG/poly(dA) looped dsDNA complex had a structure similar to the viral genome in that the dsDNA ends were able to induce transfection and protection. The present work identifies the SPG and poly(dA) looped minimum-sized gene combination as a candidate for a non-toxic gene delivery system. (c) 2005 Elsevier B.V. All rights reserved.
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Cholesterol-appended beta-(1 -> 3)-D-glucan schizophylllan for antisense oligonucleotides delivery to enhance the cellular uptake
K Koumoto, M Mizu, T Anada, T Nagasaki, S Shinkai, K Sakurai
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 78 ( 10 ) 1821 - 1830 2005.10
Publisher：CHEMICAL SOC JAPAN
Schizophyllan is a natural beta-(1 -> 3)-D-glucan existing as a triple helix in water and as a single chain in dimethyl sulfoxide (DMSO), respectively. As we already reported, when a homo-polynucleotide is added to a schizophyllan solution. the single chain of schizophyllan forms a complex with the polynucleotide. One of the potential applications of this novel complex is an antisense-oligonucleotide (AS ODN) carrier. The present paper describes a modification technique that enables us to introduce a cholesterol group only to the side chain of schizophyllan. We prepared four cholesterol-appended schizophyllans with different modification levels. Using these compounds, we made complexes and carried out an in vitro antisense assay, administrating a phosphorothioate AS ODN to the several cell lines to depress their c-myb mRNA. When we used 2.2-2.3 mol % modified schizophyllan as the carrier, the antisense effect was most enhanced among others. The addition of beta-cyclodextrin improved the complexation ability as well as the up-take for highly modified samples. Furthermore, the cytotoxicity for these modified schizophyllan samples was negligibly as small as the natural (unmodified) schizophyllan. The present work has thus clarified that schizophyllan can act as a new potential candidate for AS ODN carriers.
DOI： 10.1246/bcsj.78.1821
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一分子蛍光エネルギー移動（FRET）を利用したバイオ高分子の動的挙動解析
川上純司
高分子 54 ( 9 ) 703 2005.9
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (international conference proceedings) Publisher：高分子学会
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PEG-appended beta-(1 -> 3)-D-glucan schizophyllan to deliver antisense-oligonucleotides with avoiding lysosomal degradation
R Karinaga, K Koumoto, M Mizu, T Anada, S Shinkai, K Sakurai
BIOMATERIALS 26 ( 23 ) 4866 - 4873 2005.8
Publisher：ELSEVIER SCI LTD
Schizophyllan is a natural beta-(1 -> 3)-D-glucan existing as a triple helix in water and as a single chain in dimethylsulfoxide (DMSO). As we already reported, when a homo-polynucleotide [e.g., poly(dA) or poly(C)] is added to the schizophyllan/DMSO solution and subsequently DMSO is exchanged for water, the single chain of schizophyllan forms a complex with the polynucleotide. One of the potential applications for this novel complex is an antisense-oligonucleotide (AS ODN) carrier. The present paper describes a modification technique that enabled us to introduce PEG only to the side chain of schizophyllan. This technique consisted of periodate oxidation of the glucose side chain and subsequent reaction between methoxypolyethylene glycol amine and the formyl terminate, followed by reduction with NaBH4. Subsequently, we made a complex from PEG-appended schizophyllan and an AS ODN sequence, and carried out an in vitro antisense assay, administrating the AS ODN complex to depress A375 c-myb mRNA of A375 melanoma cell lines. The PEG-SPG/AS ODN complex showed more enhanced antisnese effect than naked AS ODN dose, i.e., the same level as that of RGD-appended SPG. Here, the RGD system has been shown one on the most effective AS ODN carrier (Science 261 (1993) 1004-1012). When we added nigericin to the assay system, the antisense effect was not affected in the PEG-SPG system, on the other hand, it was almost eliminated in the RGD system. Nigericin is well known to interrupt transport from endosome to lysosome. Therefore, the difference between the PEG and RGD complexes indicates that, in the PEG system, AS ODN was able to escape from lysosomal degradation. The present work has thus proposed a new strategy to delivery AS ODN using schizophyllan as a new carrier. (C) 2004 Elsevier Ltd. All rights reserved.
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Parallel vs. anti-parallel orientation in a curdlan/oligo(dA) complex as estimated by a FRET technique
M Numata, K Koumoto, M Mizu, K Sakurai, S Shinkai
ORGANIC & BIOMOLECULAR CHEMISTRY 3 ( 12 ) 2255 - 2261 2005.6
Publisher：ROYAL SOC CHEMISTRY
We already found that beta-1,3-glucan polysaccharides form polymeric complexes with certain polynucleotides, but the parallel vs. anti-parallel orientation in those complexes had remained unsolved. In this paper, this controversial problem has been discussed for curdlan/oligo(dA) complexes utilizing two different energy transfer techniques. The first system consists of a combination of fluorescein-labeled curdlan and 3'-(or 5'-)tetramethyl-rhodamine (TAMRA)-labeled oligo(dA). The second system utilizes gold nanoparticles: that is, two curdlan chains were linked by a disulfide bond and after complexation with oligo(dA), the complex was immobilized on gold nanoparticles. In this system, TAMRA was attached to the 3' (or 5') end of oligo(dA) and the gold particle acted as a fluorescence quencher (energy acceptor). These experiments have led us to conclude that in the curdlan/oligo(dA) complex, parallel orientation is more favourable than anti-parallel orientation. These findings have enabled us to envision a clearer image for the complexation mode between beta-1,3-glucan polysaccharides and polynucleotides.
DOI： 10.1039/b500156k
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Construction of a PNA-DNA Hybrid Array Potential for a Novel Addressable Chip Reviewed
SANO Shusuke, USUI Kenji, TOMIZAKI Kin-ya, MIHARA Hisakazu
2004 387 - 388 2005.3
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Peptide Microarrays Using Designed Libraries for Protein Detection Reviewed
MIHARA Hisakazu, USUI Kenji, OJIMA Tetsunori, SUZUKI Masato, WATANABE Sin-ya, TOMIZAKI Kin-ya, NOKIHARA Kiyoshi
2004 113 - 114 2005.3
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Gene delivery system using polysaccharide schizophyllan and poly(DA) tailed DNA.
T Anada, R Karinaga, K Koumoto, M Mizu, S Shinkai, K Sakurai
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 229 U1160 - U1160 2005.3
Publishing type：Research paper, summary (international conference) Publisher：AMER CHEMICAL SOC
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In situ monitoring of polysaccharide-polynucleotide interaction using a schizophyllan-immobilized QCM device
DH Yang, AH Bae, K Koumoto, SW Lee, K Sakurai, S Shinkai
SENSORS AND ACTUATORS B-CHEMICAL 105 ( 2 ) 490 - 494 2005.3
Publisher：ELSEVIER SCIENCE SA
Schizophyllan belongs to the beta-(1 -> 3)-D-glucan family and is well known as a triple helix polysaccharide. It is already known that the single chain of schizophyllan (s-SPG) can specifically form a complex with particular polynucleotides. As an application of this recognition capability, a novel schizophyllan derivative connected with a disulfide bond through along methylene spacer was synthesized, and its interaction with polynucleoticles was directly monitored with a self-assembled monolayer on QCM. The gold-coated QCM electrode was covered in ca. 60% as a monolayer of s-SPG. The QCM resonator showed sensitive mass increases for poly(C) and poly(U) in the presence of K+ ion, which are consistent with our previous results. The present results indicate that the schizophyllan-immobilized QCM resonator can be a new sensing device for biomolecules. (c) 2004 Elsevier B.V. All rights reserved.
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Studies on Fluorescent Dyes and Surface Chemistry focusing on Practical Peptide Array Preparation
NOKIHARA Kiyoshi, USUI Kenji, YONEMURA Koichi, OHYAMA Takafumi, OKA Yasuo, TOMIZAKI Kin‐ya, MIHARA Hisakazu
Pept Sci 2004 145 - 148 2005.3