論文 - 三好 大輔
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Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA. 査読あり 国際誌
Hisae Tateishi-Karimata, Dipanwita Banerjee, Tatsuya Ohyama, Saki Matsumoto, Daisuke Miyoshi, Shu-Ich Nakano, Naoki Sugimoto
Biochemical and biophysical research communications 2020年2月
Telomeric G-quadruplex topology has the ability to regulate telomerase activity, which counteracts the shortening of telomere with successive cell divisions, thereby causing genomic longevity. However, the detailed mechanism of G-quadruplexes topologies formed by telomeric sequences requires further investigation. In this study, we quantitatively investigated the effect of cosolutes, particularly the varying number of hydroxyl groups, on the structural transition between hybrid type and parallel G-quadruplexes formed by telomeric DNA sequences. Cosolutes with one or no hydroxyl groups in the vicinal position more efficiently induced the transition to parallel G-quadruplex from hybrid G-quadruplex than those with more hydroxyl groups. We also examined the effect of cosolute structures on the hydration of G-quadruplex formation; the results indicated that cosolutes with fewer hydroxyl groups lead to the release of greater amount of water during G-quadruplex formation. Molecular dynamics results showed that the parallel G-quadruplex was more dehydrated than the hybrid type G-quadruplex. Generally, a dehydrated structure is favored under crowding condition. Thus, depending on the surrounding cosolutes, the G-quadruplex topology can be controlled by the G-quadruplex hydration state.
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Shikhar Tyagi, Sarika Saxena, Nikita Kundu, Taniya Sharma, Amlan Chakraborty, Sarvpreet Kaur, Daisuke Miyoshi, Jadala Shankaraswamy
RSC Advances 9 40255 - 40262 2019年12月
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We described a novel synthetic peptide in which a glutamine residue binds through hydrogen bonding to a guanine-base and a trytophan residue intercalates with K+ resulting in stabilization of a human telomeric G-quadruplex with high selectivity over its complementary c-rich strand and a double-stranded DNA and its complementary C-rich strand. This peptide offers great potential for cancer treatment by inhibiting the telomere extension by telomerase.
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The RAS mRNA G-quadruplex: making undruggable cancer targets druggable
Keiko Kawauchi, Wataru Sugimoto, Daisuke Miyoshi
The Atlas of Science 2019年10月
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細胞の分子夾雑環境における核酸構造と核酸構造リガンドの挙動 ～細胞内でも望みの機能を発現する分子の合理設計に向けて～
三好大輔
生命化学研究レター 58 ( 1 ) 17 - 21 2019年4月
単著
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Osmolyte-Enhanced Protein Synthesis Activity of a Reconstituted Translation System 査読あり
Yoshiki Moriizumi, Kazuhito V. Tabata, Daisuke Miyoshi, Hiroyuki Noji
ACS Synth Biol. 8 ( 3 ) 557 - 567 2019年2月
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Metal sensitive and DNA concentration dependent structural rearrangement of short oligonucleotide into large suprastructures 査読あり
J. Shankaraswamy, Shikhar Tyagi, Anju Singh, Daisuke Miyoshi, Sarika Saxena
Journal of Biomolecular Structure and Dynamics 37 ( 9 ) 2211 - 2218 2018年12月
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An anionic phthalocyanine decreases NRAS expression by breaking down its RNA G-quadruplex 査読あり
Keiko Kawauchi, Wataru Sugimoto, Takatoshi Yasui, Kohei Murata, Katsuhiko Itoh, Kazuki Takagi, Takaaki Tsuruoka, Kensuke Akamatsu, Hisae Tateishi-Karimata, Naoki Sugimoto, and Daisuke Miyoshi
Nature Communicationsvolume 9 2271 2018年6月
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DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity 査読あり
Kenji Usu, Arisa Okada, Shungo Sakashita, Masayuki Shimooka, Takaaki Tsuruoka, Shu-ichi Nakano, Daisuke Miyoshi, Tsukasa Mashima, Masato Katahira and Yoshio Hamada
Molecules 22 1991 2017年11月
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Highly Sensitive Telomerase Assay Insusceptible to Telomerase and Polymerase Chain Reaction Inhibitors for Cervical Cancer Screening Using Scraped Cells. 査読あり
Hidenobu Yaku , Yoshio Yoshida, Hidehiko Okazawa, Yasushi Kiyono, Yuko Fujita, Daisuke Miyoshi
Anal. Chem. 89 ( 13 ) 6948 - 6953 2017年5月
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Cell and Molecular Mechanics in Health and Disease 招待あり 査読あり
Keiko Kawauchi, Hideaki Fujita, Daisuke Miyoshi, Evelyn K. F. Yim, and Hiroaki Hirata
BioMed Research International Article ID 2860241 2017年2月
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DOI： 10.1155/2017/2860241
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Unexpected Position-Dependent Effects of Ribose G‑Quartets in G‑Quadruplexes 査読あり
J. Zhou, S. Amrane, F. Rosu, G.F. Salgado, Y. Bian, H. Tateishi-Karimata, E. Largy, D. N. Korkut, A. Bourdoncle, D. Miyoshi, J. Zhang, H. Ju, W. Wang, N. Sugimoto, V. Gabelica, and J. Mergny
J. Am. Chem. Soc. 139 7768 - 7779 2017年
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Selective and Robust Stabilization of Triplex DNA Structures Using Cationic Comb-type Copolymers. 査読あり
Yamayoshi A, Miyoshi D, Zouzumi YK, Matsuyama Y, Ariyoshi J, Shimada N, Murakami A, Wada T, Maruyama A
J Phys Chem B 121 ( 16 ) 4015 - 4022 2017年
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担当区分：筆頭著者
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Reevaluation of the stability of G-quadruplex structures under crowding conditions 査読あり
Jun Zhou, Hisae Tateishi-Karimata, Jean-Louis Mergny, Mingpan Cheng, Zhaochi Feng, Daisuke Miyoshi, Naoki Sugimoto, Can Li
Biochimie 121 204 - 208 2016年
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A reversible B-A transition of DNA duplexes induced by synthetic cationic copolymers 査読あり
Nonoka Yamaguchi, Yu-ki Zouzumi, Naohiko Shimada, Shuichi Nakano, Naoki Sugimoto, Atsushi Maruyama, Daisuke Miyoshi
Chem. Commun. 52 7446 - 7449 2016年
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担当区分：筆頭著者
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Effects of background anionic compounds on the activity of the hammerhead ribozyme in Mg2+-unsaturated solutions 査読あり
Shu-ichi Nakano, Yuichi Kitagawa, Daisuke Miyoshi, Naoki Sugimoto
J. Biol. Inorg. Chem. 20 1049 - 1058 2015年
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Stabilization of DNA Structures With Poly(ethylene sodium phosphate) 査読あり
R. Moriyama, Y. Iwasaki, D. Miyoshi
J. Phys. Chem. B 119 11969 - 11977 2015年
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Effects of cosolvents on the folding and catalytic activities of the hammerhead ribozyme 査読あり
S. Nakano, Y. Kitagawa, H. Yamashita, D. Miyoshi, N. Sugimoto
ChemBioChem 16 1803 - 1810 2015年
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