論文 - 三好 大輔
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Liquid-liquid phase separation induced by i-motif DNA under molecular crowding conditions 招待あり 査読あり
Ryosuke Suzuki, Mitsuki Tsuruta, Sumit Shil, Kosei Morohashi, Keiko Kawauchi, Daisuke Miyoshia
Polymer J. 2025年4月
担当区分：責任著者
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Three- and four-stranded nucleic acid structures and their ligands 招待あり 査読あり
Yoshiki Hashimoto, Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, and Daisuke Miyoshi
RSC Chemical Biology 6 466 - 491 2025年3月
担当区分：責任著者
DOI： 10.1039/D4CB00287C
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ケミカルバイオロジーの冒険（１２） 核酸を標的とした低分子・中分子化合物 招待あり
堂野主税、勝田陽介、三好大輔
現代化学 648 32 - 39 2025年2月
担当区分：最終著者
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Roles of Loop Region in Folding Kinetics and Transcription Inhibition of DNA G-Quadruplexes 査読あり
Minori Nakata, Naoki Kosaka, Keiko Kawauchi, Daisuke Miyoshi
Biochemistry 64 609 - 619 2025年1月
担当区分：責任著者
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NRAS DNA G-quadruplex-targeting molecules for sequence-selective enzyme inhibition 招待あり 査読あり
Yoshiki Hashimoto, Hiroki Kubo, Keiko Kawauchi and Daisuke Miyoshi
Chem. Commun. 2024年10月
担当区分：責任著者
DOI： 10.1039/D4CC03753G
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The role of cytosine methylation in regulating the topology and liquid-liquid phase separation of DNA G-quadruplexes 査読あり
Mitsuki Tsuruta, Sumit Shil, Shinya Taniguchi, Keiko Kawauchi and Daisuke Miyoshi
Chem. Sci. 16 4213 - 4225 2024年10月
担当区分：責任著者
DOI： 10.1039/D4SC06959E
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Factors Affecting Liquid-Liquid Phase Separation of RGG Peptides with DNA G-Quadruplex 招待あり 査読あり
Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, Daisuke Miyoshi
ChemMedChem 2024年9月
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Bioinformatic Analysis of Actin-Binding Proteins in the Nucleolus During Heat Shock 招待あり 査読あり
Shinya Taniguchi, Takeru Torii ,Toshiyuki Goto, Kohei Takeuchi, Rine Katsumi, Mako Sumida, Sunmin Lee, Wataru Sugimoto, Masaya Gessho, Katsuhiko Itoh, Hiroaki Hirata, Junji Kawakami, Daisuke Miyoshi and Keiko Kawauchi
Genes 15 ( 12 ) 1580 2024年9月
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Quantitative Effects of the Loop Region on Topology, Thermodynamics, and Cation Binding of DNA G-quadruplexes 査読あり
Minori Nakata, Naoki Kosaka, Keiko Kawauchi, Daisuke Miyoshi
ACS Omega 9 35028 - 35036 2024年7月
担当区分：最終著者, 責任著者
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細胞内環境で安定化する核酸構造を標的にした低分子薬の開発 招待あり
橋本 佳樹, 川内 敬子, 三好 大輔
MEDCHEM NEWS (日本薬学会） 34 ( 1 ) 36 - 42 2024年4月
担当区分：最終著者, 責任著者
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Loss of p53 function promotes DNA damage-induced formation of nuclear actin filaments 査読あり 国際誌
Takeru Torii, Wataru Sugimoto, Katsuhiko Itoh, Natsuki Kinoshita, Masaya Gessho, Toshiyuki Goto, Ikuno Uehara, Wataru Nakajima, Yemima Budirahardja, Daisuke Miyoshi, Takahito Nishikata, Nobuyuki Tanaka, Hiroaki Hirata, Keiko Kawauchi
Cell Death & Disease 14 ( 11 ) 766766 - 766 2023年8月
Tumor suppressor p53 plays a central role in response to DNA damage. DNA-damaging agents modulate nuclear actin dynamics, influencing cell behaviors; however, whether p53 affects the formation of nuclear actin filaments remains unclear. In this study, we found that p53 depletion promoted the formation of nuclear actin filaments in response to DNA-damaging agents, such as doxorubicin (DOXO) and etoposide (VP16). Even though the genetic probes used for the detection of nuclear actin filaments exerted a promotive effect on actin polymerization, the detected formation of nuclear actin filaments was highly dependent on both p53 depletion and DNA damage. Whilst active p53 is known to promote caspase-1 expression, the overexpression of caspase-1 reduced DNA damage-induced formation of nuclear actin filaments in p53-depleted cells. In contrast, co-treatment with DOXO and the pan-caspase inhibitor Q-VD-OPh or the caspase-1 inhibitor Z-YVAD-FMK induced the formation of nuclear actin filament formation even in cells bearing wild-type p53. These results suggest that the p53-caspase-1 axis suppresses DNA damage-induced formation of nuclear actin filaments. In addition, we found that the expression of nLifeact-GFP, the filamentous-actin-binding peptide Lifeact fused with the nuclear localization signal (NLS) and GFP, modulated the structure of nuclear actin filaments to be phalloidin-stainable in p53-depleted cells treated with the DNA-damaging agent, altering the chromatin structure and reducing the transcriptional activity. The level of phosphorylated H2AX (γH2AX), a marker of DNA damage, in these cells also reduced upon nLifeact-GFP expression, whilst details of the functional relationship between the formation of nLifeact-GFP-decorated nuclear actin filaments and DNA repair remained to be elucidated. Considering that the loss of p53 is associated with cancer progression, the results of this study raise a possibility that the artificial reinforcement of nuclear actin filaments by nLifeact-GFP may enhance the cytotoxic effect of DNA-damaging agents in aggressive cancer cells through a reduction in gene transcription.
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The iron chelator deferriferrichrysin induces paraptosis via extracellular signal-related kinase activation in cancer cells 招待あり 査読あり
Natsuki Kinoshita, Masaya Gessho, Takeru Torii, Yukako Ashida, Minori Akamatsu, Alvin Kunyao Guo, Sunmin Lee, Tatsuya Katsuno 3, Wataru Nakajima Yemima Budirahardja, Daisuke Miyoshi, Takehiko Todokoro, Hiroki Ishida, Takahito Nishikata, Keiko Kawauchi
Genes Cells 2023年6月
DOI： 10.1111/gtc.13053
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Quantification of the concentration in a droplet formed by liquid–liquid phase separation of G-quadruplex-forming RNA 査読あり
Kohei Yokosawa, Mitsuki Tsuruta, Shinji Kajimoto, Naoki Sugimoto, Daisuke Miyoshi, Takakazu Nakabayashi
Chem. Phys. Lett. 826 140634 - 140634 2023年6月
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Biomolecular Liquid–Liquid Phase Separation for Biotechnology 招待あり 査読あり
Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi and Daisuke Miyoshi
BioTech 12 ( 2 ) 26 2023年3月
担当区分：責任著者
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Simple and fast screening for structure-selective G-quadruplex ligands 査読あり 国際誌
Yoshiki Hashimoto, Yoshiki Imagawa, Kaho Nagano, Ryuichi Maeda, Naho Nagahama, Takeru Torii, Natsuki Kinoshita, Nagisa Takamiya, Keiko Kawauchi, Hisae Tatesishi-Karimata, Naoki Sugimoto and Daisuke Miyoshi
Chem. Commun. 59 ( 33 ) 4891 - 4894 2023年2月
担当区分：責任著者
Structural selectivity of G-quadruplex ligands is essential for cellular applications since there is an excess of nucleic acids forming duplex structures compared to G-quadruplex structures in living cells. In this study, we developed new structure-selective G-quadruplex ligands utilizing a simple and fast screening system. The affinity, selectivity, enzymatic inhibitory activity and cytotoxicity of the structure-selective G-quadruplex ligands were demonstrated along with a structural selectivity-cytotoxicity relationship of G-quadruplex ligands.
DOI： 10.1039/D3CC00556A
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RNA 高次構造を標的とした光線力学療法の展望 招待あり
取井猛流・木下菜月・橋本佳樹・杉本渉・川内敬子・三 好大輔
38 ( 13 ) 1154 - 1156 2023年
担当区分：最終著者, 責任著者
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グアニン四重らせん構造による転移因子LINE-1の発現抑制名
月生 雅也, 李 先民, Yemima Suryani Budirahardja, 鶴田 充生, 橋本 佳樹, 高宮 渚, 木下 菜月, 建石 寿枝, 杉本 直己, 三好 大輔, 川内 敬子
日本女性科学者の会学術誌 23 48 - 48 2023年
出版者・発行元：(一社)日本女性科学者の会
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Controlling liquid–liquid phase separation of G-quadruplex-forming RNAs in a sequence-specific manner 査読あり 国際誌
Mitsuki Tsuruta, Takeru Torii, Kazuki Kohata, Keiko Kawauchi, Hisae Tateishi-Karimata, Naoki Sugimoto and Daisuke Miyoshi
Chem. Commun. 58 ( 93 ) 12931 - 12934 2022年11月
We constructed a minimum liquid-liquid phase separation model system to form liquid droplets using only G-quadruplex-forming oligonucleotides and R- and G-rich oligopeptides. We found that the G-quadruplex structure is an essential component for RNA to form droplets with the peptide. Based on this model system and our findings, droplet redissolution via structure transition from a G-quadruplex to a duplex was achieved in a sequence-specific manner.
DOI： 10.1039/D2CC04366A
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細胞内環境で安定化される核酸非構造を狙った分子標的薬の開発 招待あり
川内敬子・橋本佳樹・杉本 渉・三好大輔
バイオマテリアル = Journal of Japanese Society for Biomaterials : 生体材料 40 ( 3 ) 206 - 211 2022年7月
担当区分：責任著者
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核酸の化学修飾と構造や相分離能の相関 招待あり
鶴田 充生・取井 猛流・杉本 渉・川内 敬子・三好 大輔
月刊「細胞」（ニューサイエンス社） 53 ( 14 ) 910 - 913 2022年6月
担当区分：最終著者, 責任著者