論文 - 三好 大輔
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Identification of Ligand-Responsive RNA G-Quadruplexes in the 3′ UTRs of Dengue Virus Serotypes 査読あり
Mohammad Jafar Sheikhi, Ayuka Onuma, Yutaro Imachi, Akira Shiraishi, Shoko Mori, Kohtaro Sugahara, Daisuke Miyoshi, Yue Ma, Takayuki Hishiki, Kazuo Nagasawa and Masayuki Tera
Biomolecules 2026年6月
DOI： 10.3390/biom16070946
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Exclusion of RNA-binding domains from G-quadruplex condensates by G-quadruplex ligands 招待あり 査読あり 国際共著
Yoshiki Hashimoto, Ryosuke Suzuki, Mizuho Aya, Nagisa Takamiya, Mitsuki Tsuruta,Takeru Torii, Toshiyuki Goto, Keiko Kawauchi and Daisuke Miyoshi
RSC Chemical Biology 2026年6月
担当区分：最終著者, 責任著者
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Loss of p53 Provokes NF-κB-Dependent Disruption of Nucleolar Cap and Nucleoplasmic Redistribution of Fibrillarin During Nucleolar Stress
Takeru Torii, Mako Sumida, Atsushi Kobayashi, Toshiyuki Goto, Ryosuke Suzuki, Shin Kuwamoto, Wataru Nakajima, Wataru Sugimoto, Kohei Takeuchi, Yuma Tanaya, Masayuki Tera, Nobuyuki Tanaka, Hiroaki Hirata, Hisae Tateishi-Karimata, Takahito Nishikata, Miwako Kato Homma, Daisuke Miyoshi, Keiko Kawauchi
BIOMOLECULES 16 ( 2 ) 2026年2月
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Investigation of the physicochemical and functional properties of poly(2-methacryloyloxyethyl phosphorylcholine)-conjugated aptamers. 国際誌
Seojung Cho, Jumpei Morimoto, Yutaro Saito, Yukiko Nagai, Asuka Sakata, Keitaro Yoshimoto, Mitsuki Tsuruta, Daisuke Miyoshi, Shinsuke Sando
Biomaterials science 14 ( 1 ) 232 - 239 2026年1月
Polymer conjugation is a common strategy to improve the pharmacokinetics of aptamers, yet its effects on aptamer properties are incompletely understood. Poly(ethylene glycol) (PEG) is the most widely used polymer for this purpose, but concerns about anti-PEG immune responses have prompted interest in alternative polymers. We previously reported that conjugation with the zwitterionic polymer poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) significantly prolongs the circulation time of a DNA aptamer while avoiding anti-PEG antibody recognition. In this study, we evaluated the physicochemical and functional consequences of PMPC conjugation of aptamers. Biophysical analyses suggested that the secondary structure and target-binding affinity of the aptamer were preserved, while functional consequences upon PMPC conjugation varied with the targets. The activity of a membrane receptor-targeting aptamer partially decreased, likely due to spatial constraints around the cell membrane, while RB005, targeting soluble activated coagulation factor IX, retained its full activity. In addition, PMPC conjugation significantly prolonged the in vivo plasma retention of RB005. By elucidating the effects of PMPC on aptamer properties and introducing another example that further supports the general applicability of PMPC conjugation in enhancing aptamer pharmacokinetics, these findings support PMPC as a promising alternative to PEG.
DOI： 10.1039/d5bm01078k
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Phase Separation of RX Repeat Peptides with Nucleic Acids. 国際誌
Sumit Shil, Mitsuki Tsuruta, Ryosuke Suzuki, Yoshiki Hashimoto, Takeru Torii, Shinya Taniguchi, Tomohiro Umetani, Keiko Kawauchi, Daisuke Miyoshi
Chemistry, an Asian journal 20 ( 23 ) e00805 2025年12月
Biomolecular liquid-liquid phase separation (LLPS) plays a crucial role in organizing membraneless cellular compartments, which regulate a wide variety of cellular processes. A key molecular mechanism underlying LLPS of nucleic acids involves G-quadruplex (G4) structures of DNA and RNA interacting with intrinsically disordered proteins, particularly arginine and glycine (RGG/RG) rich proteins. The role of arginine residues in LLPS has been studied extensively, whereas few studies have focused on the role of the another frequently occurring residues, glycine. Here, we systematically investigated the contribution of G residues by substituting them with alanine (A), proline (P), valine (V), and tyrosine (Y) residues, generating a series of RX repeat peptides. Turbidity and microscopy assays with DNA oligonucleotides forming G4, duplex, as well as random coil, showed that RP and RA-peptides enhanced LLPS with G4 DNA, by comparing RG-peptide. In contrast, RY promoted liquid-solid phase separation (LSPS) with the G4 DNA, although it underwent LLPS with the random coil and duplex DNAs. In addition, RV-peptide formed aggregates even in the absence of any DNA. These results demonstrate that side-chain size, hydrophobicity, and aromaticity are critical factors for the LLPS and LSPS capability and selectivity with DNA forming various secondary structures. This study provides mechanistic insights into protein-nucleic acid LLPS and LSPS and guides the rational design peptides to undergo LLPS but not LSPS with nucleic acids.
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Heterogeneity of Molecular Crowding and Liquid-Liquid Phase Separation. 国際誌
Mitsuki Tsuruta, Sumit Shil, Keiko Kawauchi, Daisuke Miyoshi
Sub-cellular biochemistry 109 327 - 345 2025年10月
担当区分：責任著者
The inside of a living cell is highly crowded with extremely diverse biomacromolecules, small metabolites and osmolytes. The molecular conditions in cells change dynamically and rapidly depending on the cell cycle and state, organelle, and compartment. Much remains unknown regarding how biomolecular interactions and reactions can proceed in a spatiotemporally specific manner in such crowded, heterogeneous, and dynamic molecular environments. Selective condensation/droplet formation of biomolecules via liquid-liquid phase separation may be critical for interactions and reactions inside cells. In this chapter, we briefly describe the heterogeneity of molecular environments inside cells and the biological roles of liquid-liquid phase separation that allows biomolecular interactions and reactions in such heterogenous molecular environments. Finally, we discuss the mutual relationship between molecular crowding and liquid-liquid phase separation.
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Liquid-liquid phase separation induced by i-motif DNA under molecular crowding conditions 招待あり 査読あり
Ryosuke Suzuki, Mitsuki Tsuruta, Sumit Shil, Kosei Morohashi, Keiko Kawauchi, Daisuke Miyoshia
Polymer J. 57 931 - 940 2025年4月
担当区分：責任著者
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Three- and four-stranded nucleic acid structures and their ligands 招待あり 査読あり 国際誌
Yoshiki Hashimoto, Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, and Daisuke Miyoshi
RSC Chemical Biology 6 ( 4 ) 466 - 491 2025年3月
担当区分：責任著者
Nucleic acids have the potential to form not only duplexes, but also various non-canonical secondary structures in living cells. Non-canonical structures play regulatory functions mainly in the central dogma. Therefore, nucleic acid targeting molecules are potential novel therapeutic drugs that can target 'undruggable' proteins in various diseases. One of the concerns of small molecules targeting nucleic acids is selectivity, because nucleic acids have only four different building blocks. Three- and four-stranded non-canonical structures, triplexes and quadruplexes, respectively, are promising targets of small molecules because their three-dimensional structures are significantly different from the canonical duplexes, which are the most abundant in cells. Here, we describe some basic properties of the triplexes and quadruplexes and small molecules targeting the triplexes and tetraplexes.
DOI： 10.1039/D4CB00287C
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ケミカルバイオロジーの冒険（１２） 核酸を標的とした低分子・中分子化合物 招待あり
堂野主税、勝田陽介、三好大輔
現代化学 648 32 - 39 2025年2月
担当区分：最終著者
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Roles of Loop Region in Folding Kinetics and Transcription Inhibition of DNA G-Quadruplexes 査読あり
Minori Nakata, Naoki Kosaka, Keiko Kawauchi, Daisuke Miyoshi
Biochemistry 64 609 - 619 2025年1月
担当区分：責任著者
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NRAS DNA G-quadruplex-targeting molecules for sequence-selective enzyme inhibition 招待あり 査読あり
Yoshiki Hashimoto, Hiroki Kubo, Keiko Kawauchi and Daisuke Miyoshi
Chem. Commun. 60 13179 - 13182 2024年10月
担当区分：責任著者
DOI： 10.1039/D4CC03753G
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The role of cytosine methylation in regulating the topology and liquid-liquid phase separation of DNA G-quadruplexes 査読あり 国際誌
Mitsuki Tsuruta, Sumit Shil, Shinya Taniguchi, Keiko Kawauchi and Daisuke Miyoshi
Chem. Sci. 16 ( 10 ) 4213 - 4225 2024年10月
担当区分：責任著者
Aberrant expansion of GGGGCC DNA repeats that form G-quadruplexes (G4) is the main cause of amyotrophic lateral sclerosis (ALS). Expanded GGGGCC repeats induce liquid-liquid phase separation (LLPS) through their interaction with cellular proteins. Furthermore, GGGGCC expansion induces cytosine methylation (mC). Previous studies have shown that even slight chemical modifications of RNAs and proteins can drastically affect their LLPS ability, yet the relationship between LLPS and epigenetic DNA modifications like mC remains unexplored. As a model system, we investigated the effects of mC on LLPS induced by GGGGCC repeat DNAs and show for the first time that mC suppresses LLPS by altering the topology of G4 from being parallel to antiparallel.
DOI： 10.1039/D4SC06959E
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Factors Affecting Liquid-Liquid Phase Separation of RGG Peptides with DNA G-Quadruplex 招待あり 査読あり
Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, Daisuke Miyoshi
ChemMedChem 2024年9月
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Bioinformatic Analysis of Actin-Binding Proteins in the Nucleolus During Heat Shock 招待あり 査読あり 国際誌
Shinya Taniguchi, Takeru Torii ,Toshiyuki Goto, Kohei Takeuchi, Rine Katsumi, Mako Sumida, Sunmin Lee, Wataru Sugimoto, Masaya Gessho, Katsuhiko Itoh, Hiroaki Hirata, Junji Kawakami, Daisuke Miyoshi and Keiko Kawauchi
Genes 15 ( 12 ) 1580 2024年9月
BACKGROUND/OBJECTIVES: Actin plays a crucial role not only in the cytoplasm, but also in the nucleus, influencing various cellular behaviors, including cell migration and gene expression. Recent studies reveal that nuclear actin dynamics is altered by cellular stresses, such as DNA damage; however, the effect of heat shock on nuclear actin dynamics, particularly in the nucleolus, remains unclear. This study aims to elucidate the contribution of nucleolar actin to cellular responses under heat shock conditions. METHODS: Nuclear actin dynamics in response to heat shock were investigated using nAC-GFP, a GFP-tagged actin chromobody, to visualize nuclear actin in HeLa cells. Bioinformatic analyses were also performed. RESULTS: Heat shock induced the reversible assembly of nAC-GFP in the nucleolus, with disassembly occurring upon recovery in a heat shock protein (Hsp) 70-dependent manner. Because the nucleolus, formed via liquid-liquid phase separation (LLPS), sequesters misfolded proteins under heat shock to prevent irreversible aggregation, we hypothesized that nucleolar actin-binding proteins might also be sequestered in a similar manner. Using several databases, we identified 47 actin-binding proteins localized in the nucleolus and determined the proportion of intrinsically disordered regions (IDRs) known to promote LLPS. Our analysis revealed that many of these 47 proteins exhibited high levels of IDRs. CONCLUSIONS: The findings from our bioinformatics analysis and further cellular studies may help elucidate new roles for actin in the heat shock response.
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Quantitative Effects of the Loop Region on Topology, Thermodynamics, and Cation Binding of DNA G-quadruplexes 査読あり 国際誌
Minori Nakata, Naoki Kosaka, Keiko Kawauchi, Daisuke Miyoshi
ACS Omega 9 ( 32 ) 35028 - 35036 2024年7月
担当区分：最終著者, 責任著者
The thermal stability of G-quadruplexes is important for their biological roles. G-quadruplexes are stable in the presence of cations such as K+ and Na+ because these cations coordinate in the G-quartet of four guanine bases. It is well known that the number of G-quartets and the configuration of the guanine bases affect the binding affinity of the cation. Recently, structures formed in the loop regions connecting the guanine stretches have attracted significant attention, because the loop region affects G-quadruplex properties, such as topology, thermal stability, and interactions with proteins and small molecules. Considering these effects, the loop region can also affect the binding affinity of the cations. Here, we designed a series of G-quadruplex-forming DNA sequences that contain a hairpin in a loop region and investigated the effects of the sequence and structure of the loop region on the cation binding affinity as well as the thermal stability of the G-quadruplex as a whole. First, structural analysis of the DNA sequences showed that the hairpin at the loop plays a key role in determining G4 topology (strand orientation). Second, in the case of the G-quadruplexes with the hairpin-forming loop region, it was found that a longer loop length led to a higher thermodynamic stability of the G-quadruplex as well as higher cation binding affinity. In contrast, an unstructured loop region did not lead to such effects. Interestingly, the cation binding affinity was correlated to the thermodynamic stability of the hairpin structure at the loop region. It was quantitatively demonstrated that the stable loop region stabilized the whole G-quadruplex structure, which induced higher cation binding affinity. These systematic and quantitative results showed that the loop region is one of the determinants of cation binding and expanded the possibilities of drug development targeting G4s by stabilizing the loop region.
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細胞内環境で安定化する核酸構造を標的にした低分子薬の開発 招待あり
橋本 佳樹, 川内 敬子, 三好 大輔
MEDCHEM NEWS (日本薬学会） 34 ( 1 ) 36 - 42 2024年4月
担当区分：責任著者
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RNA 高次構造を標的とした光線力学療法の展望 招待あり
取井猛流・木下菜月・橋本佳樹・杉本渉・川内敬子・三 好大輔
38 ( 13 ) 1154 - 1156 2023年11月
担当区分：最終著者, 責任著者
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Loss of p53 function promotes DNA damage-induced formation of nuclear actin filaments 査読あり 国際誌
Takeru Torii, Wataru Sugimoto, Katsuhiko Itoh, Natsuki Kinoshita, Masaya Gessho, Toshiyuki Goto, Ikuno Uehara, Wataru Nakajima, Yemima Budirahardja, Daisuke Miyoshi, Takahito Nishikata, Nobuyuki Tanaka, Hiroaki Hirata, Keiko Kawauchi
Cell Death & Disease 14 ( 11 ) 766766 - 766 2023年8月
Tumor suppressor p53 plays a central role in response to DNA damage. DNA-damaging agents modulate nuclear actin dynamics, influencing cell behaviors; however, whether p53 affects the formation of nuclear actin filaments remains unclear. In this study, we found that p53 depletion promoted the formation of nuclear actin filaments in response to DNA-damaging agents, such as doxorubicin (DOXO) and etoposide (VP16). Even though the genetic probes used for the detection of nuclear actin filaments exerted a promotive effect on actin polymerization, the detected formation of nuclear actin filaments was highly dependent on both p53 depletion and DNA damage. Whilst active p53 is known to promote caspase-1 expression, the overexpression of caspase-1 reduced DNA damage-induced formation of nuclear actin filaments in p53-depleted cells. In contrast, co-treatment with DOXO and the pan-caspase inhibitor Q-VD-OPh or the caspase-1 inhibitor Z-YVAD-FMK induced the formation of nuclear actin filament formation even in cells bearing wild-type p53. These results suggest that the p53-caspase-1 axis suppresses DNA damage-induced formation of nuclear actin filaments. In addition, we found that the expression of nLifeact-GFP, the filamentous-actin-binding peptide Lifeact fused with the nuclear localization signal (NLS) and GFP, modulated the structure of nuclear actin filaments to be phalloidin-stainable in p53-depleted cells treated with the DNA-damaging agent, altering the chromatin structure and reducing the transcriptional activity. The level of phosphorylated H2AX (γH2AX), a marker of DNA damage, in these cells also reduced upon nLifeact-GFP expression, whilst details of the functional relationship between the formation of nLifeact-GFP-decorated nuclear actin filaments and DNA repair remained to be elucidated. Considering that the loss of p53 is associated with cancer progression, the results of this study raise a possibility that the artificial reinforcement of nuclear actin filaments by nLifeact-GFP may enhance the cytotoxic effect of DNA-damaging agents in aggressive cancer cells through a reduction in gene transcription.
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The iron chelator deferriferrichrysin induces paraptosis via extracellular signal-related kinase activation in cancer cells 招待あり 査読あり 国際誌
Natsuki Kinoshita, Masaya Gessho, Takeru Torii, Yukako Ashida, Minori Akamatsu, Alvin Kunyao Guo, Sunmin Lee, Tatsuya Katsuno 3, Wataru Nakajima Yemima Budirahardja, Daisuke Miyoshi, Takehiko Todokoro, Hiroki Ishida, Takahito Nishikata, Keiko Kawauchi
Genes Cells 28 ( 9 ) 653 - 662 2023年6月
Cancer cells generally exhibit increased iron uptake, which contributes to their abnormal growth and metastatic ability. Iron chelators have thus recently attracted attention as potential anticancer agents. Here, we show that deferriferrichrysin (Dfcy), a natural product from Aspergillus oryzae acts as an iron chelator to induce paraptosis (a programmed cell death pathway characterized by ER dilation) in MCF-7 human breast cancer cells and H1299 human lung cancer cells. We first examined the anticancer efficacy of Dfcy in cancer cells and found that Dfcy induced ER dilation and reduced the number of viable cells. Extracellular signal-related kinase (ERK) was activated by Dfcy treatment, and the MEK inhibitor U0126, a small molecule commonly used to inhibit ERK activity, prevented the increase in ER dilation in Dfcy-treated cells. Concomitantly, the decrease in the number of viable cells upon treatment with Dfcy was attenuated by U0126. Taken together, these results demonstrate that the iron chelator Dfcy exhibits anticancer effects via induction of ERK-dependent paraptosis.
DOI： 10.1111/gtc.13053
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Quantification of the concentration in a droplet formed by liquid–liquid phase separation of G-quadruplex-forming RNA 査読あり
Kohei Yokosawa, Mitsuki Tsuruta, Shinji Kajimoto, Naoki Sugimoto, Daisuke Miyoshi, Takakazu Nakabayashi
Chem. Phys. Lett. 826 140634 - 140634 2023年6月
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Biomolecular Liquid–Liquid Phase Separation for Biotechnology 招待あり 査読あり
Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi and Daisuke Miyoshi
BioTech 12 ( 2 ) 26 2023年3月
担当区分：責任著者
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グアニン四重らせん構造による転移因子LINE-1の発現制御 招待あり
月生 雅也, 李 先民, Yemima Suryani Budirahardja, 鶴田 充生, 橋本 佳樹, 高宮 渚, 木下 菜月, 建石 寿枝, 杉本 直己, 三好 大輔, 川内 敬子
日本女性科学者の会学術誌 23 48 - 48 2023年3月
出版者・発行元：(一社)日本女性科学者の会
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Simple and fast screening for structure-selective G-quadruplex ligands 査読あり 国際誌
Yoshiki Hashimoto, Yoshiki Imagawa, Kaho Nagano, Ryuichi Maeda, Naho Nagahama, Takeru Torii, Natsuki Kinoshita, Nagisa Takamiya, Keiko Kawauchi, Hisae Tatesishi-Karimata, Naoki Sugimoto and Daisuke Miyoshi
Chem. Commun. 59 ( 33 ) 4891 - 4894 2023年2月
担当区分：責任著者
Structural selectivity of G-quadruplex ligands is essential for cellular applications since there is an excess of nucleic acids forming duplex structures compared to G-quadruplex structures in living cells. In this study, we developed new structure-selective G-quadruplex ligands utilizing a simple and fast screening system. The affinity, selectivity, enzymatic inhibitory activity and cytotoxicity of the structure-selective G-quadruplex ligands were demonstrated along with a structural selectivity-cytotoxicity relationship of G-quadruplex ligands.
DOI： 10.1039/D3CC00556A
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Controlling liquid–liquid phase separation of G-quadruplex-forming RNAs in a sequence-specific manner 査読あり 国際誌
Mitsuki Tsuruta, Takeru Torii, Kazuki Kohata, Keiko Kawauchi, Hisae Tateishi-Karimata, Naoki Sugimoto and Daisuke Miyoshi
Chem. Commun. 58 ( 93 ) 12931 - 12934 2022年11月
We constructed a minimum liquid-liquid phase separation model system to form liquid droplets using only G-quadruplex-forming oligonucleotides and R- and G-rich oligopeptides. We found that the G-quadruplex structure is an essential component for RNA to form droplets with the peptide. Based on this model system and our findings, droplet redissolution via structure transition from a G-quadruplex to a duplex was achieved in a sequence-specific manner.
DOI： 10.1039/D2CC04366A
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rDNAおよびrRNAが形成する四重らせん構造による核小体機能の制御 招待あり
川内敬子、取井猛流、月生雅也、木下菜月、三好大輔
月刊細胞 54 ( 12 ) 36 - 39 2022年11月
担当区分：責任著者
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細胞内環境で安定化される核酸非構造を狙った分子標的薬の開発 招待あり
川内敬子・橋本佳樹・杉本 渉・三好大輔
バイオマテリアル = Journal of Japanese Society for Biomaterials : 生体材料 40 ( 3 ) 206 - 211 2022年7月
担当区分：責任著者
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核酸の化学修飾と構造や相分離能の相関 招待あり
鶴田 充生・取井 猛流・杉本 渉・川内 敬子・三好 大輔
月刊「細胞」（ニューサイエンス社） 53 ( 14 ) 910 - 913 2022年6月
担当区分：最終著者, 責任著者
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Significant structural change in human c-Myc promoter G-quadruplex upon peptide binding in potassium 査読あり 国際共著
Nikita Kundu, Taniya Sharma, Sarvpreet Kaur, Mamta Singh, Vinit Kumar, Uttam Sharma, Aklank Jain, Jadala Shankaraswamy, Daisuke Miyoshi, Sarika Saxena
RSC Adv. 12 7594 - 7604 2022年4月
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核酸の四重らせん構造を狙った治療薬の展望 招待あり
木下菜月、取井猛流、川内敬子、三好大輔
37 ( 4 ) 52 - 55 2022年3月
担当区分：責任著者
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Intramolecular G-quadruplex-hairpin loop structure competition of a GC-rich exon region in the TMPRSS2 gene 査読あり
Wataru Sugimoto, Natsuki Kinoshita, Minori Nakata, Tatsuya Ohyama, Hisae Minatojima-Minaminachi, Takahito Nishikata, Naoki Sugimoto, Daisuke Miyoshi, Keiko Kawauchi
Chem. Commun. 58 48 - 51 2022年1月
担当区分：責任著者
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Detection of Intracellular Reactive Oxidative Species Using the Fluorescent Probe Hydroxyphenyl Fluorescein 招待あり 査読あり
Wataru Sugimoto, Daisuke Miyoshi, Keiko Kawauchi
Methods in Molecular Biology 2274 207 - 215 2021年5月
出版者・発行元：Humana Press Inc.
Various fluorescent probes for the detection of intracellular reactive oxidative species (ROS) have been developed because ROS levels are closely associated with cellular states. Here, we describe a method for detection of intracellular ROS in living cells using the fluorescent probe, hydroxyphenyl fluorescein (HPF), which detects hydroxyl radicals and peroxynitrite. NIH3T3 cells and p53 knockout (p53−/−) mouse embryonic fibroblasts (MEFs) were transformed by expressing oncogenic RAS using a retrovirus system. The cells were treated with HPF at 37 °C for 30 min, and subsequently, images were acquired using a confocal fluorescence microscope at an excitation wavelength of 488 nm after washing with PBS.
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がん関連RNAを破壊する分子標的型光線力学療法 招待あり 査読あり
杉本 渉・橋本 佳樹・川内 敬子・三好 大輔
BIO Clinica 36 ( 5 ) 65 - 67 2021年5月
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核小体ストレスにより形成するrDNA G-quadruplex集合体とその機能
杉本 渉, 桑本 慎, 建石 寿枝, 岩根 敦子, 高橋 宏隆, 三好 大輔, 川内 敬子
日本薬学会年会要旨集 141年会 29V05 - am10S 2021年3月
出版者・発行元：(公社)日本薬学会
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Combined Effects of Methylated Cytosine and Molecular Crowding on the Thermodynamic Stability of DNA Duplexes 招待あり 査読あり
Combined Effects of Methylated Cytosine and Molecular Crowding on the Thermodynamic Stability of DNA Duplexes
Int. J. Mol. Sci. 22 ( 2 ) 947 2021年1月
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Photosensitizers Based on G-Quadruplex Ligand for Cancer Photodynamic Therapy 招待あり 国際誌
Keiko Kawauchi, Ryoto Urano, Natsuki Kinoshita, Shin Kuwamoto, Takeru Torii, Yoshiki Hashimoto, Shinya Taniguchi, Mitsuki Tsuruta, Daisuke Miyoshi
Genes 11 ( 11 ) 1340 2020年11月
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TakahiroYamauchi, Takafumi Kubodera, Daisuke Miyoshi, Naoki Sugimoto, Shuji Hirohata
Journal of Bioscience and Bioengineering 2020年10月
共著
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杉本 渉, 川内 敬子, 三好 大輔
Medical Science Digest 46 ( 9 ) 26 - 27 2020年7月
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Takeru Torii, Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi
Journal of Data Mining in Genomics & Proteomics 2020年6月
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Kazuki Kohata, Daisuke Miyoshi
Biophys. Rev. 12 669 - 676 2020年5月
共著
Living cells are highly crowded with large and small biomolecules. The total concentration of biomolecules can reach 400 mg/ml, and 40% of the cell volume is occupied by biomolecules. Droplet formation in cells via liquid-liquid phase separation may play a role in controlling biochemical reactions in this complex molecular environment. Liquid-liquid phase separation generally involves nucleic acids and proteins as anionic and cationic components, respectively. Significant characteristics of droplets, which make them different from protein aggregation or fibril formation, are reversibility of formation and responsiveness to the molecular environment. In this review, we quantitatively describe the molecular environment inside cells and droplets that participate in controlling central dogma reactions. Finally, we discuss the importance of droplets under conditions of molecular crowding within living cells.
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Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA 査読あり
H. Tateishi-Karimata, D. Banerjee, T. Ohyama, S. Matsumoto, D. Miyoshi, S. Nakano, and N. Sugimoto
Biochemical and Biophysical Research Communications 525 ( 1 ) 177 - 183 2020年4月
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Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA. 査読あり 国際誌
Hisae Tateishi-Karimata, Dipanwita Banerjee, Tatsuya Ohyama, Saki Matsumoto, Daisuke Miyoshi, Shu-Ich Nakano, Naoki Sugimoto
Biochemical and biophysical research communications 2020年2月
Telomeric G-quadruplex topology has the ability to regulate telomerase activity, which counteracts the shortening of telomere with successive cell divisions, thereby causing genomic longevity. However, the detailed mechanism of G-quadruplexes topologies formed by telomeric sequences requires further investigation. In this study, we quantitatively investigated the effect of cosolutes, particularly the varying number of hydroxyl groups, on the structural transition between hybrid type and parallel G-quadruplexes formed by telomeric DNA sequences. Cosolutes with one or no hydroxyl groups in the vicinal position more efficiently induced the transition to parallel G-quadruplex from hybrid G-quadruplex than those with more hydroxyl groups. We also examined the effect of cosolute structures on the hydration of G-quadruplex formation; the results indicated that cosolutes with fewer hydroxyl groups lead to the release of greater amount of water during G-quadruplex formation. Molecular dynamics results showed that the parallel G-quadruplex was more dehydrated than the hybrid type G-quadruplex. Generally, a dehydrated structure is favored under crowding condition. Thus, depending on the surrounding cosolutes, the G-quadruplex topology can be controlled by the G-quadruplex hydration state.
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Shikhar Tyagi, Sarika Saxena, Nikita Kundu, Taniya Sharma, Amlan Chakraborty, Sarvpreet Kaur, Daisuke Miyoshi, Jadala Shankaraswamy
RSC Advances 9 40255 - 40262 2019年12月
共著
We described a novel synthetic peptide in which a glutamine residue binds through hydrogen bonding to a guanine-base and a trytophan residue intercalates with K+ resulting in stabilization of a human telomeric G-quadruplex with high selectivity over its complementary c-rich strand and a double-stranded DNA and its complementary C-rich strand. This peptide offers great potential for cancer treatment by inhibiting the telomere extension by telomerase.
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The RAS mRNA G-quadruplex: making undruggable cancer targets druggable
Keiko Kawauchi, Wataru Sugimoto, Daisuke Miyoshi
The Atlas of Science 2019年10月
共著
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細胞の分子夾雑環境における核酸構造と核酸構造リガンドの挙動 ～細胞内でも望みの機能を発現する分子の合理設計に向けて～
三好大輔
生命化学研究レター 58 ( 1 ) 17 - 21 2019年4月
単著
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Osmolyte-Enhanced Protein Synthesis Activity of a Reconstituted Translation System 査読あり
Yoshiki Moriizumi, Kazuhito V. Tabata, Daisuke Miyoshi, Hiroyuki Noji
ACS Synth Biol. 8 ( 3 ) 557 - 567 2019年2月
共著
-
Metal sensitive and DNA concentration dependent structural rearrangement of short oligonucleotide into large suprastructures 査読あり
J. Shankaraswamy, Shikhar Tyagi, Anju Singh, Daisuke Miyoshi, Sarika Saxena
Journal of Biomolecular Structure and Dynamics 37 ( 9 ) 2211 - 2218 2018年12月
共著
-
An anionic phthalocyanine decreases NRAS expression by breaking down its RNA G-quadruplex 査読あり
Keiko Kawauchi, Wataru Sugimoto, Takatoshi Yasui, Kohei Murata, Katsuhiko Itoh, Kazuki Takagi, Takaaki Tsuruoka, Kensuke Akamatsu, Hisae Tateishi-Karimata, Naoki Sugimoto, and Daisuke Miyoshi
Nature Communicationsvolume 9 2271 2018年6月
-
DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity 査読あり
Kenji Usu, Arisa Okada, Shungo Sakashita, Masayuki Shimooka, Takaaki Tsuruoka, Shu-ichi Nakano, Daisuke Miyoshi, Tsukasa Mashima, Masato Katahira and Yoshio Hamada
Molecules 22 1991 2017年11月
-
Highly Sensitive Telomerase Assay Insusceptible to Telomerase and Polymerase Chain Reaction Inhibitors for Cervical Cancer Screening Using Scraped Cells. 査読あり
Hidenobu Yaku , Yoshio Yoshida, Hidehiko Okazawa, Yasushi Kiyono, Yuko Fujita, Daisuke Miyoshi
Anal. Chem. 89 ( 13 ) 6948 - 6953 2017年5月
共著
-
Cell and Molecular Mechanics in Health and Disease 招待あり 査読あり
Keiko Kawauchi, Hideaki Fujita, Daisuke Miyoshi, Evelyn K. F. Yim, and Hiroaki Hirata
BioMed Research International Article ID 2860241 2017年2月
共著
DOI： 10.1155/2017/2860241
-
Unexpected Position-Dependent Effects of Ribose G‑Quartets in G‑Quadruplexes 査読あり
J. Zhou, S. Amrane, F. Rosu, G.F. Salgado, Y. Bian, H. Tateishi-Karimata, E. Largy, D. N. Korkut, A. Bourdoncle, D. Miyoshi, J. Zhang, H. Ju, W. Wang, N. Sugimoto, V. Gabelica, and J. Mergny
J. Am. Chem. Soc. 139 7768 - 7779 2017年
共著
-
Selective and Robust Stabilization of Triplex DNA Structures Using Cationic Comb-type Copolymers. 査読あり
Yamayoshi A, Miyoshi D, Zouzumi YK, Matsuyama Y, Ariyoshi J, Shimada N, Murakami A, Wada T, Maruyama A
J Phys Chem B 121 ( 16 ) 4015 - 4022 2017年
共著
担当区分：筆頭著者
-
Reevaluation of the stability of G-quadruplex structures under crowding conditions 査読あり
Jun Zhou, Hisae Tateishi-Karimata, Jean-Louis Mergny, Mingpan Cheng, Zhaochi Feng, Daisuke Miyoshi, Naoki Sugimoto, Can Li
Biochimie 121 204 - 208 2016年
共著
-
A reversible B-A transition of DNA duplexes induced by synthetic cationic copolymers 査読あり
Nonoka Yamaguchi, Yu-ki Zouzumi, Naohiko Shimada, Shuichi Nakano, Naoki Sugimoto, Atsushi Maruyama, Daisuke Miyoshi
Chem. Commun. 52 7446 - 7449 2016年
共著
担当区分：筆頭著者
-
Effects of background anionic compounds on the activity of the hammerhead ribozyme in Mg2+-unsaturated solutions 査読あり
Shu-ichi Nakano, Yuichi Kitagawa, Daisuke Miyoshi, Naoki Sugimoto
J. Biol. Inorg. Chem. 20 1049 - 1058 2015年
共著
-
Stabilization of DNA Structures With Poly(ethylene sodium phosphate) 査読あり
R. Moriyama, Y. Iwasaki, D. Miyoshi
J. Phys. Chem. B 119 11969 - 11977 2015年
共著
-
Effects of cosolvents on the folding and catalytic activities of the hammerhead ribozyme 査読あり
S. Nakano, Y. Kitagawa, H. Yamashita, D. Miyoshi, N. Sugimoto
ChemBioChem 16 1803 - 1810 2015年
共著
-
A mRNA-responsive G-quadruplex-based drug release system 招待あり 査読あり
Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, and Naoki Sugimoto
Sensors 15 ( 4 ) 9388 - 9403 2015年
共著
担当区分：筆頭著者
-
DNA G-quadruplex detection system employing a protein fibril ligand 招待あり 査読あり
Ryuichi Maeda, Hidenobu Yaku, Takakazu Nakabayashi, Takashi Murashima, Naoki Sugimoto, Daisuke Miyoshi
Telomere and Telomerase 2 e691 2015年
共著
担当区分：筆頭著者
-
DNA structures under molecular crowding conditions with a phosphorylcholine derivative (MPC) 査読あり
Yu-mi Ueda, Tomohiro Konno, Kazuhiko Ishihara, Naoki Sugimoto, Daisuke Miyoshi
Transact. Mat. Res. Soc. Japan 40 99 - 102 2015年
共著
担当区分：筆頭著者
-
DNA structures under molecular crowding conditions with a phosphorylcholine derivative (MPC)
Yu-mi Ueda, Tomohiro Konno, Kazuhiko Ishihara, Naoki Sugimoto, Daisuke Miyoshi
Transact. Mat. Res. Soc. Japan 40 99 - 102 2015年
共著
-
Stabilization of DNA Structures With Poly(ethylene sodium phosphate)
R. Moriyama, Y. Iwasaki, D. Miyoshi
J. Phys. Chem. B 119 11969 - 11977 2015年
共著
-
A fluorescent probe for detection of an intracellular prognostic indicator in early-stage cancer (10.1039/c4cc08789e 査読あり
Haiwei Ji, Yijia Guan, L Wu, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto Xiaogang Qu
Chem. Commun. 51 1479 - 1482 2014年
共著
-
Preparation of hydrogels for the study of the effects of spatial confinement on DNA 査読あり
Shu-ichi Nakano, Masatoshi Yoshida, Daisuke Yamaguchi, Naoki Sugimoto
Transact. Mat. Res. Soc. Japan 39 435 - 438 2014年
共著
-
Dangling ends perturb the stability of RNA duplex responsive to surrounding condition 招待あり 査読あり
H. Tateishi-Karimata, S. Pramanik, S. Nakano, D. Miyoshi and N. Sugimoto
ChemMedChem 9 ( 9 ) 2150 - 2155 2014年
共著
-
Hammerhead ribozyme activity and oligonucleotide duplex stability in mixed solutions of water and organic compounds 査読あり
Shu-ichi Nakano, Yuichi Kitagawa, Daisuke Miyoshi, Naoki Sugimoto
FEBS Open Bio 4 643 - 650 2014年
共著
-
Drastic stabilization of parallel DNA hybridizations by a polylysine comb-type copolymer with hydrophilic graft chain 査読あり
Daisuke Miyoshi, Yu-mi Ueda, Naohiko Shimada, Shu-ichi Nakano, Naoki Sugimoto, Atsushi Maruyama
ChemMedChem 9 ( 9 ) 2156 - 2163 2014年
共著
担当区分：筆頭著者
-
グラフェンと生体分子を用いたバイオセンサー 招待あり 査読あり
三好大輔、上田侑美
バイオサイエンスとインダストリー 72 ( 2 ) 117 - 120 2014年
共著
担当区分：筆頭著者
-
measure” FRET-based telomeric tandem repeat sequence detection and telomerase assay method 査読あり
Koji Kawamura, Hidenobu Yaku, Daisuke Miyoshi, Takashi Murashima
Org. Biomol. Chem. 12 934 - 941 2014年
共著
-
Effects of Molecular Crowding on the Structures, Interactions, and Functions of Nucleic Acids 査読あり
Shu-ichi Nakano, Daisuke Miyoshi, Naoki Sugimoto
Chem. Rev. 114 ( 5 ) 2733 - 2758 2014年
共著
担当区分：筆頭著者
-
In Vitro Assays Predicting of Telomerase Inhibitory Effect of G-Quadruplex Ligands in Cell Nuclei 査読あり
Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, Naoki Sugimoto
J. Phys. Chem. B 118 2605 - 2614 2014年
共著
担当区分：筆頭著者
-
Reduced Graphene Oxide Upconversion Nanoparticle Hybrid for Electrochemiluminescent Sensing of a Prognostic Indicator in Early-Stage Cancer 査読あり
Li Wu, Jiasi Wang, Meili Yin, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, Xiaogang Qu
Small 10 330 - 336 2014年
共著
-
Preparation of hydrogels for the study of the effects of spatial confinement on DNA
Shu-ichi Nakano, Masatoshi Yoshida, Daisuke Yamaguchi, Naoki Sugimoto
Transact. Mat. Res. Soc. Japan 39 435 - 438 2014年
共著
-
Thermodynamics - Hydration Relationships within Loops that Affect G-Quadruplexes under Molecular Crowding Conditions 査読あり
Takeshi Fujimoto, Shu-ichi Nakano, Naoki Sugimoto, Daisuke Miyoshi
J. Phys. Chem. B 117 963 - 972 2013年1月
共著
担当区分：筆頭著者
-
A Highly Sensitive Telomerase Activity Assay that Eliminates False-Negative Results Caused by PCR Inhibitors 査読あり
Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, and Naoki Sugimoto
Molecules 18 ( 10 ) 11751 - 11767 2013年
共著
担当区分：筆頭著者
-
Sequence and Solvent Effects on Telomeric DNA Bimolecular G-Quadruplex Folding Kinetics 査読あり
Adrien Marchand, Ruben Ferreira, Hisae Tateishi-Karimata, Daisuke Miyoshi, Naoki Sugimoto, Valerie Gabelica
J. Phys. Chem. B 117 ( 41 ) 12391 - 12401 2013年
共著
-
Multiple and cooperative binding of fluorescence light-up probe Thioflavin T with human telomere DNA G-quadruplex 査読あり
Valerie Gabelica, Ryuichi Maeda, Takeshi Fujimoto, Hidenobu Yaku, Takashi Murashima, Naoki Sugimoto, and Daisuke Miyoshi
Biochemistry 52 5620 - 5628 2013年
共著
担当区分：筆頭著者
-
The beads-on-a-string structure of long telomeric DNAs under molecular crowding conditions 査読あり
Haiqing Yu, Gu Xiaobo Gu, Shu-ichi Nakano, Daisuke Miyoshi, Naoki Sugimoto
J. Am. Chem. Soc. (Higthlighted as the cover and introduced in the Spotslights on Recent JACS Publications) 134 20060 - 20069 2012年12月
共著
担当区分：筆頭著者
-
Aptamer carbon nanodot sandwich used for fluorescent detection of protein 査読あり
Bailu Xu, Chuanqi Zhao, Weili Wei, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, and Xiaogang Qu
Analyst 137 5483 - 5486 2012年12月
共著
-
Specific Binding of Anionic Porphyrin and Phthalocyanine to the G-quadruplex 招待あり 査読あり
Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, Naoki
Molecules 17 10586 - 10613 2012年8月
共著
-
Detection of a Prognostic Indicator in Early-Stage Cancer Using Functionalized Graphene-Based Peptide Sensors 査読あり
Lingyan Feng, Li Wu, Jiasi Wang, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, Xiaogang Qu
Advanced Materials 24 ( 1 ) 125 - 131 2012年1月
共著
-
細胞内環境因子に応答する核酸デバイスの開発 招待あり
三好大輔
化学と工業（日本化学会） 64 ( 12 ) 956 - 956 2011年12月
単著
担当区分：筆頭著者
-
Effects of protein binding on DNA G-quadruplex structures 招待あり
S. Nagatoishi, D. Miyoshi and N. Sugimoto
Int. Rev. Biophys. Chem. 2 129 - 134 2011年12月
共著
-
Synthesis and Application of Functional Nucleic Acids 招待あり
Daisuke Miyoshi, Friedrich C. Simmel, Souvik Maiti, and Luis A. Marky
Journal of Nucleic Acids 2011 547245 2011年12月
共著
担当区分：筆頭著者
-
Rational design of a new IMP aptamer based on a TPP riboswitch and a hypoxanthine aptamer 査読あり
Misato Tokui, Takahiro Yamauchi, Daisuke Miyoshi, Hisaya Kamiya, Akira Nishimura, Naoki Sugimoto
Chemistry Letters 40 ( 11 ) 1313 - 1314 2011年11月
共著
担当区分：筆頭著者
-
Measurements of the binding of a large protein using a substrate density-controlled DNA chip 査読あり
S. Nakano, T. Kanzaki, M. Nakano, D. Miyoshi, N. Sugimoto
Analytical Chemistry 83 6368 - 6372 2011年11月
共著
-
グラフェンを用いたバイオセンサー 招待あり
三好大輔、杉本直己
化学 66 ( 8 ) 30 - 35 2011年9月
共著
担当区分：筆頭著者
-
The effects of molecular crowding on the structure and stability of G-quadruplexes with an abasic site 査読あり
Takeshi Fujimoto, Shu Ichi Nakano, Daisuke Miyoshi, and Naoki Sugimoto
J. Nucleic Acids 2011 857149 2011年9月
共著
担当区分：筆頭著者
-
Label-free colorimetric and quantitative detection of cancer marker protein using noncrosslinking aggregation of Au/Ag nanoparticles induced by target-specific peptide probe 査読あり
Xiaohui Wang, Li Wu, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, and Xiaogang Qu
Biosensors and Bioelectronics 26 4804 - 4809 2011年8月
共著
-
Thermodynamic Stability of Hoogsteen and Watson-Crick Base Pairs in the Presence of Histone H3-Mimicking Peptide 査読あり
Smritimoy Pramanik, Kaori Nakamura, Kenji Usui, Shu-ichi Nakano, Sarika Saxena, Jun Matsuia, Daisuke Miyoshi, Naoki Sugimoto
Chem. Commun. 47 2790 - 2792 2011年2月
共著
担当区分：筆頭著者
-
Ultrasensitive and Selective Detection of a Prognostic Indicator in Early-Stage Cancer Using Graphene Oxide and Carbon Nanotubes 査読あり
Xiaohui Wang, Chunyan Wang, Konggang Qu, Yujun Song, Jinsong Ren, Daisuke Miyoshi, Naoki Sugimoto, and Xiaogang Qu
Advanced Functional Materials 20 3967 - 3971 2010年12月
共著
-
A rapid and sensitive "add-mix-measure" assay for multiple proteinases based on one goldnanoparticle-peptide-fluorophore conjugate 査読あり
X.Wang, J.Geng, D.Miyoshi, J.Ren, N.Sugimoto, and X.Qu
Biosens. Bioelectron. 26 743 - 747 2010年11月
共著
-
Sole and stable RNA duplexes of G-rich sequences located in the 5'-untranslated region of protooncogenes 査読あり
Sarika Saxena, Daisuke Miyoshi, Naoki Sugimoto
Biochemistry 49 7190 - 7201 2010年9月
共著
担当区分：筆頭著者
-
Development of new functional nanostructures consisting of both DNA duplex and quadruplex 査読あり
Kakoli Dutta, Takeshi Fujimoto, Mamiko Inoue, Daisuke Miyoshi, and Naoki Sugimoto
Chem. Commun 46 7772 - 7774 2010年9月
共著
担当区分：筆頭著者
-
Anionic Phthalocyanines Targeting G-quadruplexes and Inhibiting Telomerase Activity in the Presence of Excessive DNA Duplexes 査読あり
Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, Naoki Sugimoto
Chem. Commun 46 5740 - 5742 2010年8月
共著
担当区分：筆頭著者
-
Monomorphic RNA G-quadruplex and polymorphic DNA G-quadruplex structures responding to cellular environmental factors 査読あり
Dong-Hao Zhang, Takeshi Fujimoto, Sarika Saxena, Hai-Qing Yu, Daisuke Miyoshi, and Naoki Sugimoto
Biochemisry 49 4554 - 4563 2010年7月
共著
担当区分：筆頭著者
-
Utilization of salmon milt DNA against UV damage 査読あり
Yoshiharu Sasaki, Daisuke Miyoshi, and Naoki Sugimoto
Applied Biochem. Biotech. 160 2458 - 2466 2010年2月
共著
-
Stabilization of Three-Way Junctions of DNA under Molecular Crowding Conditions 査読あり
Sanjukta Muhuri, Kenta Mimura, Daisuke Miyoshi, and Naoki Sugimoto
J. Am. Chem. Soc. 131 9268 - 9280 2009年12月
共著
-
Hydration of Watson-Crick base pairs and dehydration of Hoogsteen base pairs inducing structural polymorphism under molecular crowding conditions
三好大輔
J. Am. Chem. Soc. ( 131 ) 3522 - 3531 2009年11月
単著
-
Thermal stability and hydration state of DNA G-quadruplex regulated by loop regions
T. Fujimoto, D. Miyoshi, H. Tateishi-Karimata, and N. Sugimoto
Nucleic Acids Symp Ser (Oxf) 53 237 - 238 2009年9月
共著
担当区分：筆頭著者
-
DNA junction structure stabilized by molecular crowding conditions
D. Miyoshi, S. Muhuri, K. Mimura, and N. Sugimoto
Nucleic Acids Symp Ser (Oxf) 53 59 - 60 2009年9月
共著
担当区分：筆頭著者
-
DNAは細胞内で三重らせん・四重らせん構造を形成しているのか？ 招待あり
三好大輔、杉本直己
化学と生物 7 449 - 451 2009年1月
共著
-
Cu(II) ion inhibits early stages of amyloid β fibril formation in vitro
T. Fujimoto, D. Miyoshi, H. Tateishi-Karimata, and N. Sugimoto
J. Biol. Inorg. Chem., Suppl. 1 S118 2009年1月
共著
-
An Approach to Peptide-based ATP Receptors by a Combination of Random Selection, Rational Design, and Molecular Imprinting 査読あり
J. Matsui, J. Nagano, D. Miyoshi, K. Tamaki, N. Sugimoto
Biosens. Bioelectron. 25 563 - 567 2009年1月
共著
-
Telomerase activity regulated by thermodynamic stability of DNA・RNA hybrid
三好大輔
Angew. Chem. Int. Ed., ( 47 ) 9034 - 9038 2008年11月
単著
-
Small-molecule-directed assembly of DNA: a gold-nanoparticle-based strategy for screening of a DNA duplex binders
三好大輔
Adv. Mat ( 20 ) 706 - 710 2008年11月
単著
-
Riboswitches for enhancing target gene expression in eukaryotes 査読あり
T. Yamauchi, D. Miyoshi, T. Kubodera, M. Ban, A. Nishimura, and N. Sugimoto
ChemBioChem 9 1040 - 1043 2008年1月
共著
-
外部刺激に応答する機能性核酸 －核酸ならではの機能を活用したナノバイオマテリアルの設計－ 招待あり
三好大輔、杉本直己
化学工業 （化学工業社） 59 ( 11 ) 21 - 27 2008年1月
共著
-
Thermodynamics of DNA structures under molecular crowding conditions with neutral and positive charged cosolutes
D. Miyoshi, K. Nakamura, S. Muhuri, H. T. Karimata, N. Sugimoto
Nucleic Acids Symp. Ser. 52 413 - 414 2008年1月
共著
-
Cationic porphyrin induced a telomeric DNA to g-quadruplex form in water 査読あり
T. Murashima, D. Sakiyama, D. Miyoshi, M. Kuriyama, T. Yamada, T. Miyazawa, N. Sugimoto
Bioinorg Chem Appl. 294756 2008年1月
共著
-
Molecular crowding effects on structure and stability of DNA 招待あり 査読あり
D. Miyoshi and N. Sugimoto
Biochimie 90 1040 - 1051 2008年1月
共著
-
DNA四重らせん構造を細胞内環境因子で制御する
三好大輔
生体機能関連化学部会 News Letter, 22 ( 3 ) 14 - 17 2007年11月
単著
-
Synthesis, structure and thermal stability of fully hydrophobic porphyrin-DNA conjugates
三好大輔
Tetrahedron Lett ( 48 ) 8514 - 8517 2007年11月
単著
-
Effects of cosolutes on the thermodynamic stability of parallel DNA duplex and triplex
三好大輔
Nucleic Acids Symp. Ser. (Oxf), ( 51 ) 167 - 168 2007年11月
単著
-
Conformational switch of a functional nanowire based on the DNA G-quadruplex
三好大輔
Nucleic Acids Symp. Ser. (Oxf), ( 51 ) 167 - 168 2007年11月
単著
-
DNAデザイン －機能性分子開発への道しるべ－
三好大輔
化学 62 ( 9 ) 28 - 32 2007年11月
単著
-
DNAを用いたバイオ計測 －センシング核酸の開発と新展開－
三好大輔
未来材料, 7 ( 6 ) 10 - 18 2007年11月
単著
-
Regulation of DNA nucleases by molecular crowding
三好大輔
Nucleic Acids Res ( 35 ) 4086 - 4093 2007年11月
単著
-
Artificial G-wire Switch with 2,2’-Bipyridine Units Responsive to Divalent Metal Ions
三好大輔
J. Am. Chem. Soc ( 129 ) 5919 - 5925 2007年11月
単著
-
Molecular Imprinting Under Molecular Crowding Conditions: an Aid to the synthesis of a High-capacity Polymeric Sorbent for Triazine Herbicides
三好大輔
Anal. Chem ( 79 ) 1749 - 1757 2007年11月
単著
-
Hydration regulates thermodynamic stability of DNA structures under molecular crowding conditions 査読あり
D. Miyoshi, H. Karimata, and N. Sugimoto
Nucleosides, Nucleotides and Nucleic Acids 26 589 - 595 2007年1月
共著
-
Development of molecular logic gates using the structural switch of telomere DNAs
三好大輔
Nucleic Acids Symp. Ser. ( 50 ) 315 - 316 2006年11月
単著
-
Molecularly Imprinting within a Single Peptide Molecule for Synthesizing a Water-soluble ATP Receptor
三好大輔
Proceedings of the International Conference of 43rd Japanese Peptide Symposium and 4th Peptide Engineering Meeting 396 - 397 2006年11月
単著
-
Properties of long telomeric DNAs under cell-mimicking conditions
三好大輔
Nucleic Acids Symp. Ser. ( 50 ) 207 - 208 2006年11月
単著
-
Factors regulating thermodynamic stability of DNA structures under molecular crowding conditions
三好大輔
Nucleic Acids Symp. Ser ( 50 ) 203 - 204 2006年11月
単著
-
Characterization of Structure and Stability of Long Telomeric DNA G-Quadruplexes
三好大輔
J. Am. Chem. Soc., ( 128 ) 15461 - 15468 2006年11月
単著
-
DNA Logic Gates Based on Structural Polymorphism of Telomere DNA Molecules Responding to Chemical Input Signals
三好大輔
Angew. Chem. Int. Ed., ( 45 ) 7716 - 7719 2006年11月
単著
-
Hydration regulates thermodynamics of G-quadruplex formation under molecular crowding conditions
三好大輔
J. Am. Chem. Soc ( 128 ) 7957 - 7963 2006年11月
単著
-
生命を制御するのは遺伝子か環境か－細胞内環境に左右される遺伝子の構造と機能－
三好大輔
高分子 55 ( 5 ) 322 - 325 2006年11月
単著
-
Effect of molecular crowding on DNA polymerase activity
三好大輔
Biotech. J., ( 1 ) 440 - 446 2006年11月
単著
-
A DNA duplex with Extremely Enhanced Thermal Stability Based on Controlled Immobilization on Gold Nanoparticles.
三好大輔
Nano Lett., ( 6 ) 491 - 495 2006年11月
単著
-
Properties of long telomeric DNAs under cell-mimicking conditions
三好大輔
Nucleic Acids Symp. Ser. ( 50 ) 207 - 208 2006年11月
単著
-
Use of the polymorphic nature of high-order DNA structures for the design of dynamic and functional nanodevices 招待あり 査読あり
D. Miyoshi, N. Sugimoto
Recent Devel. Nucleic Acids Res. 2 49 - 70 2006年1月
共著
-
Use of the polymorphic nature of high-order DNA structures for the design of dynamic and functional nanodevices
D. Miyoshi, N. Sugimoto
Recent Devel. Nucleic Acids Res. 2 49 - 70 2006年1月
共著
-
Thermodynamic and Kinetic Analysis of Nucleic Acid Structures toward Pharmacogenomics.
三好大輔
Current Pharmacogenomics, ( 3 ) 217 - 236 2005年11月
単著
-
Structural switch of telomere DNA by pH and monovalent cation.
三好大輔
Nucleic Acids Symp. Ser ( 49 ) 243 - 244 2005年11月
単著
-
Structure and stability of DNA quadruplexes under molecular crowding conditions.
三好大輔
Nucleic Acids Symp. Ser ( 49 ) 239 - 240 2005年11月
単著
-
DNA nanowire sensitive to the surrounding condition.
三好大輔
Nucleic Acids Symp. Ser. ( 49 ) 43 - 44 2005年11月
単著
-
DNA-Based Biosensor for Monitoring pH in Vitro and in Living Cells.
三好大輔
Biochemistry ( 44 ) 7125 - 7130 2005年11月
単著
-
Roles of Mg2+ in TPP-dependent riboswitch.
三好大輔
FEBS Lett. ( 579 ) 2583 - 2588 2005年11月
単著
-
Drastic Effect of a Single Base Difference between Human and Tetrahymena Telomere Sequences on Their Structures under Molecular Crowding Conditions.
三好大輔
Angew. Chem. Int. Ed. Engl ( 44 ) 3740 - 3744 2005年11月
単著
-
SPR sensor chip for detection of small molecules using molecularly imprinted polymer with embedded gold nanoparticles.
三好大輔
Anal Chem ( 77 ) 4282 - 4285 2005年11月
単著
-
Thermodynamic and Kinetic Analysis of Nucleic Acid Structures toward Pharmacogenomics
三好大輔
Current Pharmacogenomics ( 3 ) 217 - 236 2005年11月
単著
-
Duplex Dissociation of Telomere DNA Induced by Molecular Crowding.
三好大輔
J. Am. Chem. Soc. ( 126 ) 165 - 169 2004年11月
単著
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生命分子を用いたサブナノデバイスの創製
三好大輔
未来材料(NTS) ( 4 ) 26 - 33 2004年11月
単著
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Composite of Au Nanoparticles and molecularly imprinted polymer as a sensing material
三好大輔
Anal. Chem ( 76 ) 1310 - 1315 2004年11月
単著
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Structural Competition Involving G-Quadruplex DNA and Its Complement.
三好大輔
Biochemistry ( 42 ) 11736 - 11744 2003年11月
単著
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Structural Polymorphism of Telomeric DNA Regulated by pH and Divalent Cation.
三好大輔
Nucleosides, Nucleotides, and Nucleic Acids ( 22 ) 203 - 221 2003年11月
単著
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Structural Ttransition from Antiparallel to Parallel G-Quadruplex of d(G4T4G4) Induced by Ca2+.
三好大輔
Nucleic Acids Res ( 31 ) 1156 - 1163 2003年11月
単著
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Effect of Putrescine and PEG on a Structural Transition of DNA G-Quadruplex.
三好大輔
Nucleosides, Nucleotides, and Nucleic Acids ( 22 ) 1591 - 1594 2003年11月
単著
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バイオインフォマティクスを活用した未来型生体内・外センシングシステム
三好大輔
科学と工業, ( 77 ) 413 - 419 2003年11月
単著
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Novel biomaterials derived from deoxyribozyme and NAPzyme
三好大輔
Macromol. Chem. Phys. Macromol. Symp. ( 201 ) 245 - 252 2003年11月
単著
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核酸の長鎖ダングリングエンドと四重鎖の構造形成に関する熱力学的研究
三好大輔
2003年3月
単著
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Long RNA Dangling End Has Large Energetic Contribution to Duplex Stability.
三好大輔
J. Am. Chem. Soc. ( 124 ) 10367 - 10372 2002年11月
単著
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シリーズ「生命を化学する」—モレキュラークラウディングとin cell Chemistry—
三好大輔
化学（化学同人） 57 ( 12 ) 50 - 56 2002年11月
単著
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超タンパク質材料—タンパク質活用最前線—
三好大輔
未来材料（NTS） ( 5 ) 29 - 35 2002年11月
単著
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Molecular Crowding Regulates the Structural Switch of the DNA G-quadruplex.
三好大輔
Biochemistry, ( 41 ) 15017 - 15024 2002年11月
単著
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A Stable DNA tetraloop and Its Structural Tolerance for Modification.
三好大輔
Chem. Lett 258 - 259 2001年11月
単著
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Structural transition of d(G4T4G4) from antiparallel to parallel G-quartet induced by divalent cations
三好大輔
Nucleic Acids Res. Supple ( 1 ) 259 - 260 2001年11月
単著
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Effect of Divalent Cations on Antiparallel G-Quartet Structure of d(G4T4G4).
三好大輔
FEBS Lett ( 496 ) 128 - 133 2001年11月
単著
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Development of Small Peptides Recognizing a Monosaccharide by Combinatorial Chemistry.
三好大輔
Chem. Commun 2295 - 2296 2000年11月
単著
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ゲノム化学は今3 SNPs解析と次世代の生命化学 —「ニューセントラルドグマ」の構築に向けて.
三好大輔
化学（化学同人） ( 55 ) 20 - 25 2000年11月
単著
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ゲノム化学は今2 DNAチップとバイオインフォマティクス —遺伝子の定量的解析に向けて.
三好大輔
化学（化学同人） ( 55 ) 14 - 18 2000年11月
単著
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A Novel Screening Method for Combinatorial Chemistry for Low Affinity Interactions.
三好大輔
Chem. Commun. 677 - 678 1999年11月
単著
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Selection of peptides that recognize tryptophan by combinatorial chemistry
三好大輔
Peptide Science 281 - 284 1999年11月
単著
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a-b Structural Transition of Short Oligopeptides by Water/Organic Solvent Titration.
三好大輔
Chem. Lett 637 - 638 1999年11月
単著