論文 - 甲元 一也
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Synthesis and Immunestimulating Activity of Lactobacilli-Originated Polysaccharide-Polymeric Microparticle Conjugates 査読あり
Koji Nagahama, Takayuki Kumano, Tsubasa Nakata, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
Langmuir 31 1489 - 1495 2015年
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担当区分:筆頭著者
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化学修飾技術を利用した乳酸菌模倣粒子による免疫賦活 招待あり
甲元一也、長濱宏治、松井淳
バイオインダストリー 32 ( 9 ) 42 - 47 2015年
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担当区分:筆頭著者
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Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size 査読あり
Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
Bioconjugate Chemistry 26 ( 8 ) 1775 - 1781 2015年
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担当区分:筆頭著者
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Significance of anionic functional group in betaine-type metabolite analogs on the facilitation of enzyme reactions 国際誌
Yuichi Nakagawa, Kotomi Takagi, Ryutaro Genjima, Kazuya Koumoto
Bioprocess and Biosystems Engineering 38 ( 9 ) 1811 - 1817 2015年
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Enhanced Chromogenic Sensitivity of Horseradish Peroxidase-catalyzed Oxidative Reactions in the Presence of Betaine-type Metabolite Analogs 査読あり
Kotomi Takagi, Yasuhiro Kashima, Satoshi Fujii, Kazuya Koumoto
Bulletin of the Chemical Society of Japan 88 ( 8 ) 1074 - 1082 2015年
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Mechanistic study on the facilitation of enzymatic hydrolysis by alpha-glucosidase in the presence of betaine-type metabolite analogs 査読あり
Yuichi Nakagawa, Shiro Sehata, Satoshi Fujii, Hiroaki Yamamoto, Akihiko Tsuda, Kazuya Koumoto
TETRAHEDRON 70 ( 35 ) 5895 - 5903 2014年9月
出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD
Recently we reported that betaine-type metabolite analogs structure-dependently facilitate enzymatic hydrolysis reaction for alpha-glucosidases, beta-glucosidases, and alkaliphosphatases. To understand the facilitation mechanism for enzymes, in this study we expanded the analog library and measured the properties of analog solutions. The structural investigation on alpha-glucosidase-mediated hydrolysis reaction indicated that suitable structures to facilitate the enzyme reaction efficiently should have the ammonium cation in the betaine structure possess triplicate aliphatic chains from Cl to C7 without any polar functional groups. Analyses of the solution properties revealed that such analogs possess a large hydration layer with low water density. Such a specific hydration environment is generated by the characteristic structure of the betaine-type metabolite analogs. The characteristic hydration indirectly regulates enzyme activity and stability. These findings not only increase our understanding enzyme activation by betaine-type metabolite analogs, but also will contribute to the molecular design of enzyme regulators. (C) 2014 Elsevier Ltd. All rights reserved.
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Mechanistic study on the facilitation of enzymatic hydrolysis by α-glucosidase in the presence of betaine-type metabolite analogs 査読あり
Yuichi Nakagawa, Shiro Sehata, Satoshi Fujii, Hiroaki Yamamoto, Akihiko Tsuda, Kazuya Koumoto
Tetrahedron 70 5895 - 5903 2014年
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担当区分:筆頭著者
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Cellular zwitterionic metabolite analogs simultaneously enhance reaction rate, thermostability, salt tolerance, and substrate specificity of alpha-glucosidase 査読あり
Eisuke Deguchi, Kazuya Koumoto
Bioorganic and Medicinal Chemistry 19 3128 - 3134 2011年4月
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担当区分:筆頭著者
天然の代謝産物を模倣し、化学的に合成した代謝産物アナログがα−グルコシダーゼ、β−グルコシダーゼ、アルカリフォスファターゼといった加水分解酵素の活性(反応速度、耐熱性、耐塩性、基質選択性等)を溶液に共存させるだけで向上させることを明らかとした。
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Reversible stability switching of a hairpin DNA via a photo-responsive linker unit 招待あり 査読あり
Li Wu, Kazuya Koumoto, Naoki Sugimoto
Chemical Communications 1915 - 1917 2009年4月
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ヘアピンDNAのループ部位に導入し、光異性化によってヘアピンDNA全体の安定性を制御できる新規光応答性ユニットの開発に成功した。このユニットをヘアピン部位に導入したDNAは紫外光、可視光の照射に伴って安定性が可逆的に制御することができた。
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Structural effect of synthetic zwitterionic cosolutes on the stability of DNA duplexes 査読あり
甲元一也
Tetrahedron ( 64 ) 168 - 174 2008年11月
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担当区分:筆頭著者
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Artificial G-wire Switch with 2,2’-Bipyridine Units Responsive to Divalent Metal Ions
甲元一也
J. Am. Chem. Soc ( 129 ) 5919 - 5925 2007年11月
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5)天然高分子 核酸 エネルギーデータベースに基づく核酸医薬の設計
甲元一也
絵で見てわかるナノDDS 遺伝子医学MOOK別冊(メディカルドゥ) 63 - 69 2007年11月
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Conformational switch of a functional nanowire based on the DNA G-quadruplex
甲元一也
Nucleic Acids Symp. Ser. ( 51 ) 251 - 252 2007年11月
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Delivery of antisense oligonucleotides to nuclear telomere RNA by use of a complex between polysaccharide and polynucleotide
甲元一也
Bull. Chem. Soc. Jpn ( 80 ) 1091 - 1098 2007年11月
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Schizophyllan, beta-1,3-glucan,-oligoamine conjugates as non-viral vector 査読あり
Takeshi Nagasaki, Atsushi Uno, Kazuya Koumoto, Kazuo Sakurai, Seiji Shinkai
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 234 2007年8月
出版者・発行元:AMER CHEMICAL SOC
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Galactose-PEG Dual Conjugation of b-(1→3)-D-glucan schizophyllan for antisense oligonucleotide delivery to enhance cellular uptake
甲元一也
Biomaterials, ( 27 ) 1626 - 1635 2006年11月
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CpG DNA/zymosan complex to enhance cytokine secretion owing to the cocktail effect
甲元一也
Bioorg. Med. Chem. Lett ( 16 ) 1301 - 1304 2006年11月
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In situ monitoring of polysaccharide-polynucleotide interaction using a schizophyllan-immobilized QCM device
甲元一也
Sensors & Actuators B: Chemicals ( 105 ) 490 - 494 2005年11月
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Transferrin-appended b-(1→3)-D-glucan schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake
甲元一也
J. Contr. Rel. ( 108 ) 529 - 539 2005年11月
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Transferrin-appended b-(1→3)-D-glucan schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake
甲元一也
e-J. Surface Sci. Nanotech ( 3 ) 195 - 202 2005年11月
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