論文 - 甲元 一也
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Inhibitory Effects of Shikonin Dispersion, an Extract of Lithospermum erythrorhizon Encapsulated in β-1,3-1,6 Glucan, on Streptococcus mutans and Non-Mutans Streptococci 査読あり
Ryota Nomura, Yuto Suehiro, Fumikazu Tojo, Saaya Matayoshi, Rena Okawa, Masakazu Hamada, Shuhei Naka, Michiyo Matsumoto-Nakano, Rika Unesaki, Kazuya Koumoto, Keiko Kawauchi, Takahito Nishikata, Tatsuya Akitomo, Chieko Mitsuhata, Masatoshi Yagi, Toshiro Mizoguchi, Koki Fujikawa, Taizo Taniguchi and Kazuhiko Nakano
25 1075 2024年1月
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Inhibitory Effect of Adsorption of Streptococcus mutans onto Scallop-Derived Hydroxyapatite 査読あり
Momoko Usuda, Mariko Kametani, Masakazu Hamada, Yuto Suehiro, Saaya Matayoshi, Rena Okawa, Shuhei Naka, Michiyo Matsumoto-Nakano, Tatsuya Akitomo, Chieko Mitsuhata, Kazuya Koumoto, Keiko Kawauchi, Takahito Nishikata, Masatoshi Yagi, Toshiro Mizoguchi, Koki Fujikawa, Taizo Taniguchi, Kazuhiko Nakano and Ryota Nomura
Int J Mol Sci 24 11371 2023年7月
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ポリエチレングリコールによるアトピー性皮膚炎予防の可能性
厳原美穂、甲元一也
Fragrance Journal 50 ( 11 ) 54 - 57 2022年11月
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Hydrostatic pressure-regulated cellular calcium responses 査読あり 国際誌
Minami Fukuchi, Kotaro Oyama, Hiroaki Mizuno, Akihisa Miyagawa, Kazuya Koumoto, Gaku Fukuhara
Langmuir 37 ( 2 ) 820 - 826 2021年1月
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Mechanism toward turn-on of polysaccharide-porphyrin complexes for fluorescence probes and photosensitizers in photodynamic therapy in living cells 査読あり 国際誌
Shodai Hino, Rikushi Funada, Kouta Sugikawa, Riku Kawasaki, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Atsushi Ikeda
ChemMedChem 15 ( 1 ) 1 - 12 2020年10月
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Modification of the release rate of curcumin from the cavity of renatured β-1,3-1,6-glucan nanoparticles by changing the molecular weight of the constituent polysaccharide chains 査読あり
Yuki Nonoguchi, Ayumu Kodama, Toshio Suzuki, Kazuya Koumoto
Chemistry Letters 49 ( 10 ) 1150 - 1153 2020年7月
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Solubilization of poorly water-soluble bioactive molecules in neutral aqueous media by complexation with renatured beta-1,3-1,6-glucan nanoparticles 査読あり 国際誌
Ayumu Kodama, Akifumi Nakagawa, Yuki Nonoguchi, Haruka Sakurai, Chieko Yano, Toshio Suzuki, Kazuya Koumoto
Biopolymers 111 ( 4 ) e23349 2020年2月
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Improved stability and photodynamic activity of water-soluble 5,15-diazaporphyrins incorporated in beta-(1,3-1,6)-D-glucan with on-off switch 査読あり 国際誌
Shodai Hino, Shuhei Satake, Hideyuki Shinmori, Shigeki Kawabata, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Kouta Sugikawa, Riku Kawasaki, Atsushi Ikeda
Chemistry an Asian Journal 15 ( 3 ) 365 - 370 2019年12月
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Structural effect of amine N-oxides on the facilitation of alpha-glucosidase-catalyzed hydrolysis reaction 査読あり 国際誌
Takuma Aoki, Yuichi Nakagawa, Ryutaro Genjima, Kazuya Koumoto
Bioprocess Biosys. Eng. 43 ( 3 ) 541 - 548 2019年11月
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Turn-on fluorescence and photodynamic activity of beta-(1,3-1,6)-D-glucan-complexed with porhyrin derivative inside HeLa cells 査読あり 国際誌
Shodai Hino, Rikushi Funada, Kouta Sugikawa, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Atsushi Ikeda
Photochem. Photobiol. Sci. 18 ( 12 ) 2854 - 2858 2019年11月
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研究課題設定を重視した科学技術人材育成プログラムROOTのデザインと実装
伊藤 真之, 臼井 英之, 長野 寛之, 巳波 弘佳, 甲元 一也, 蛯名 邦禎
日本科学教育学会年会論文集 43 399 - 400 2019年8月
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Atsushi Ikeda, Tatsuya Iizuka, Naotake Maekubo, Kazuyuki Nobuswa, Kouta Sugikawa, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Motofusa Akiyama
Chemistry an Asian Journal 12 ( 10 ) 1069 - 1074 2017年3月
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Motofusa Akiyama, Kouta Sugikawa, Kazuya Koumoto, Yua Kashijima, Jiawei Li, Toshio Suzuki, Takeshi Nagasaki, Atsushi Ikeda
Organic & Biomolecular Chemistry 15 ( 9 ) 1990 - 1997 2017年2月
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Lipid-membrane-incorporated arylboronate esters as agents for boron neutron capture therapy 査読あり 国際誌
Masafumi Ueda, Kengo Ashizawa, Kouta Sugikawa, Kazuya Koumoto, Takeshi Nagasaki, Atsushi Ikeda
Organic & Biomolecular Chemistry 15 ( 7 ) 1565 - 1569 2016年10月
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Increased yield of β-glucosidase-catalyzed hydrolysis reactions in the presence of betaine-type metabolite analog 査読あり 国際誌
Shiro Sehata, Ryojun Suzuki, Kazuya Koumoto
Bioprocess Biosystem Engineering 40 ( 1 ) 153 - 159 2016年9月
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Cryoprotective ability of betaine-type metabolite analogs during freezing denaturation of enzymes 国際誌
Yuichi Nakagawa, Masahiro Sota, Kazuya Koumoto
BIOTECHNOLOGY LETTERS 37 ( 8 ) 1607 - 1613 2015年8月
出版者・発行元:SPRINGER
To evaluate an analog library of betaine-type cellular metabolites, which are naturally found in polar fish for survival in subzero temperatures, for preventing denaturation of enzymes during freezing.Comparison of the cryoprotective ability of reported cryoprotectants, such as dimethylsulfoxide, glycerol, ectoine, hydroxyectoine, and trehalose, with betaine-type analogs using alpha-glucosidase revealed that analogs introducing C-3-C-6 alkyl chains into an ammonium cation retained 20 % higher activity than the control cryoprotectants at the same concentration. In particular, the analog possessing triplicate n-butyl chains showed a profound effect. It allowed retention of enzyme activity to 95 % even after 100 freeze-thaw cycles, while addition of the control cryoprotectants decreased the activity to 10-20 %. The cryoprotective ability of betaine-type analogs can be applied not only to alpha-glucosidase but also other enzymes such as beta-glucosidase, alkaline phosphatase, lactose dehydrogenase, sulfatase, and horseradish peroxidase.Synthetic betaine-type metabolite analogs possess practicable cryoprotective ability for various enzymes, and are considerably superior to previously reported cryoprotectants.
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Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size 査読あり 国際誌
Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
BIOCONJUGATE CHEMISTRY 26 ( 8 ) 1775 - 1781 2015年8月
出版者・発行元:AMER CHEMICAL SOC
The design and synthesis of materials capable of activating the immune system in a safe manner is of great interest in immunology and related fields. Lactobacilli activate the innate immune system of a host when acting as probiotics. Here, we constructed lactobacilli-mimicking materials in which polysaccharide peptidoglycan complexes (PS PGs) derived from lactobacilli were covalently conjugated to the surfaces of polymeric microparticles with a wide variety of sizes, ranging from 200 nm to 3 mu m. The artificial lactobacilli successfully stimulated macrophages without cytotoxicity. Importantly, we found that the size of artificial lactobacilli strongly influenced their immunostimulating activities, and that artificial lactobacilli of 1 mu m exhibited 10-fold higher activity than natural lactobacilli. One major advantage of the artificial lactobacilli is facile control of size, which cannot be changed in natural lactobacilli. These findings provide new insights into the design of materials for immunology as well as the molecular biology of lactobacillus.
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Mechanistic study of unusual enzyme activation caused by addition of inert betaine-type metabolite and the analogs
Yuichi Nakagawa, Kazuya Koumoto
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 249 2015年3月
出版者・発行元:AMER CHEMICAL SOC
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Synthesis and lmmunestimulating Activity of Lactobacilli-Originated Polysaccharide-Polymeric Microparticle Conjugates 査読あり 国際誌
Koji Nagahama, Takayuki Kumano, Tsubasa Nakata, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
LANGMUIR 31 ( 4 ) 1489 - 1495 2015年2月
出版者・発行元:AMER CHEMICAL SOC
The design and synthesis of biomaterials capable of activating the immune system are of interest in immunology-related fields because of their ability to tune up the immune defenses of the host. Lactobacilli are a major constituent of normal human indigenous flora, and some specific strains are known to activate the immune system of the host as probiotics. In this study, we first fabricated novel biohybrid materials in which lactobacilli (L. casei strain Shirota, LcS)-originated polysaccharide-peptidoglycan complexes (PS-PGs) are conjugated with polymeric microparticles (MPs). PS-PGs conjugated onto polymeric MPs surfaces bound its specific antibody, suggesting that PS-PGs kept their original molecular recognition ability. The PS-PGs-based hybrid MPs with an appropriate density of conjugated PS-PGs effectively induced high levels of IL-12 production from macrophages without cytotoxicity. These results suggest that LcS-originated PS-PGs could be available bio-originated materials for developing novel biomaterials capable of activating the immune system in a safe manner.
DOI: 10.1021/la5041757
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Cryoprotective ability of betaine-type metabolite analogs during freezing denaturation of enzymes 査読あり
Yuichi Nakagawa, Masahiro Sota, Kazuya Koumoto
Biotechnology Letters 37 1607 - 1613 2015年
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担当区分:筆頭著者