写真a

KOUMOTO Kazuya

Position

Professor

Degree

Doctor (Engineering)(Kyushu University), Master (Engineering)(Kyushu University)

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Graduating School 【 display / non-display

  • Kyushu University   Graduated

    - 2000

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  • Kyushu University   Faculty of Engineering   Advanced a year

    1992.4 - 1995.3

  • Kyushu University   Faculty of Engineering   Graduated

    - 1995

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Graduate School 【 display / non-display

  • Kyushu University   Graduate School, Division of Engineering   Doctor's Course   Completed

    - 2000.3

  • Kyushu University   Graduate School, Division of Engineering   Master's Course   Completed

    1995.4 - 1997.3

Campus Career 【 display / non-display

  • KONAN UNIVERSITY   Faculty of Frontiers of Innovative Research in Science and Technology   Faculty of Frontiers of Innovative Research in Science and Technology Department of Nanobiochemistry   Professor

    2018.4

  • KONAN UNIVERSITY   Faculty of Frontiers of Innovative Research in Science and Technology   Faculty of Frontiers of Innovative Research in Science and Technology Department of Nanobiochemistry   Associate Professor

    2009.4 - 2018.3

  • KONAN UNIVERSITY   Frontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry   Lecturer

    2004.4 - 2009.3

External Career 【 display / non-display

  • 甲南大学フロンティアサイエンス学部生命化学科 准教授

    2009 - 2018.3

  • 北九州市立大学国際環境工学部

    2002.4 - 2004.3

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    Country:Japan

  • 九州大学大学院応用化学部門

    2002.1 - 2002.3

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    Country:Japan

  • 科学技術振興事業団分子転写プロジェクト

    2000.4 - 2001.12

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    Country:Japan

  • 科学技術振興事業団日蘭国際共同研究分子転写プロジェクト 研究員

    2000 - 2001

 

Papers 【 display / non-display

  • Hydrostatic Pressure-Regulated Cellular Calcium Responses. Reviewed International journal

    Minami Fukuchi, Kotaro Oyama, Hiroaki Mizuno, Akihisa Miyagawa, Kazuya Koumoto, Gaku Fukuhara

    Langmuir : the ACS journal of surfaces and colloids   37 ( 2 )   820 - 826   2021.1

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    Joint Work

    Hydrostatic pressure control has attracted much attention and presents a still challenging objective from mechanobiological viewpoints. Herein, we reveal the calcium entry processes in HeLa cells by means of hydrostatic pressure spectroscopy. The steady-state fluorescence spectral data comprehensively elucidated the factors controlling the outcomes of the hydrostatic pressure-stimulated calcium entry behavior. The present work leads to a new perspective on ion regulations in living cells and an attractive alternative to conventional mechanostimuli.

    DOI: 10.1021/acs.langmuir.0c03141

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  • Mechanism toward Turn-on of Polysaccharide-Porphyrin Complexes for Fluorescence Probes and Photosensitizers in Photodynamic Therapy in Living Cells Reviewed International journal

    Shodai Hino, Rikushi Funada, Kouta Sugikawa, Riku Kawasaki, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Atsushi Ikeda

    CHEMMEDCHEM   15 ( 1 )   1 - 12   2020.10

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    Joint Work

    Publisher:WILEY-V C H VERLAG GMBH  

    beta-(1,3-1,6)-D-Glucan, lambda-carrageenan, tamarind gum, and pullulan can dissolve various porphyrin derivatives via the formation of complexes in water using a high-speed vibration milling method. The aqueous solutions of the resulting complexes exhibit long-term stability. Despite the adverse effects of the self-quenching process, notable fluorescence and improved photodynamic activity of the polysaccharide-complexed porphyrin derivatives were observed in the presence of liposomes, micelles, cyclodextrins, and HeLa cells. It was noted that the type of porphyrins was more important than the type of polysaccharides present in the complex. Porphyrin self-aggregates were monodispersed in the lipid membranes of the liposomes and lysosomes. The polysaccharide-complexed porphyrin derivatives showed increased photodynamic activity toward HeLa cells under photoirradiation between 610 and 740 nm.

    DOI: 10.1002/cmdc.202000656

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  • Modification of the Release Rate of Curcumin from the Cavity of Renatured beta-1,3-1,6-Glucan Nanoparticles by Changing the Molecular Weight of the Constituent Polysaccharide Chains Reviewed

    Yuki Nonoguchi, Ayumu Kodama, Rika Unesaki, Nanako Doi, Toshio Suzuki, Kazuya Koumoto

    CHEMISTRY LETTERS   49 ( 10 )   1150 - 1153   2020.7

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    Joint Work

    Publisher:CHEMICAL SOC JAPAN  

    We prepared renatured beta-1,3-1,6-glucan nanoparticles from molecular-weight-fractionated beta-1,3-1,6-glucans to analyze the molecular effects on the inclusion and release of the guest molecule curcumin. Glucan molecular weight strongly affects the amount of curcumin included in the complexes and its release rate. We found that the slow release rate of the incorporated curcumin is attributed not only to the stabilized stacking structure but also the slow migration between the internal and surface cavities characteristic of polymeric materials.

    DOI: 10.1246/cl.200366

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  • Solubilization of poorly water-soluble bioactive molecules in neutral aqueous media by complexation with renatured beta-1,3-1,6-glucan nanoparticles Reviewed International journal

    Ayumu Kodama, Akifumi Nakagawa, Yuki Nonoguchi, Haruka Sakurai, Chieko Yano, Toshio Suzuki, Kazuya Koumoto

    BIOPOLYMERS   111 ( 4 )   e23349   2020.2

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    Joint Work

    Publisher:WILEY  

    The design of scaffolds for solubilizing/dispersing poorly water-soluble bioactive molecules in neutral aqueous media is a major challenge of functional food, pharmaceuticals, and cosmetics development, as highlighted by the plethora of corresponding solubilization/dispersion strategies. Herein, renatured beta-1,3-1,6-glucan (r-glucan) nanoparticles prepared by neutralization of alkali-denatured beta-1,3-1,6-glucan and subsequent centrifugation are used as a host to disperse water-insoluble bioactive molecules (curcumin, all-trans-retinoic acid, and rebamipide) by simple mixing of host and guest solutions. Curcumin in the r-glucan cavity is found to be stacked in the form of J-aggregates and twisted along the helix, and is demonstrated to be retained for significantly longer than curcumin in the corresponding gamma-cyclodextrin (gamma-CD) complex. Specifically, curcumin incorporated in gamma-CD is released within 5.5 hours, whereas that in the r-glucan complex is released very slowly, with 12% of curcumin in the latter complex retained after 31-day incubation at 37 degrees C. Thus, inclusion protocol simplicity and slow release ability make r-glucan nanoparticles a potential carrier scaffold for various applications.

    DOI: 10.1002/bip.23349

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  • Improved Stability and Photodynamic Activity of Water-Soluble 5,15-Diazaporphyrins Incorporated in beta-(1,3-1,6)-d-Glucan with On-Off Switch Reviewed International journal

    Shodai Hino, Shuhei Satake, Hideyuki Shinmori, Shigeki Kawabata, Kazuya Koumoto, Toshio Suzuki, Takeshi Nagasaki, Kouta Sugikawa, Riku Kawasaki, Atsushi Ikeda

    CHEMISTRY-AN ASIAN JOURNAL   15 ( 3 )   365 - 370   2019.12

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    Joint Work

    Publisher:WILEY-V C H VERLAG GMBH  

    5,15-Diazaporphyrins, which have a large absorption at wavelengths over 600 nm, were dissolved in water by complex formation with beta-(1,3-1,6)-d-glucans. Aqueous solutions of these complexes were relatively stable compared with their trimethyl-beta-cyclodextrin-complexed analogues. beta-Glucan-complexed diazaporphyrins showed quenched fluorescence and had low singlet-oxygen-generation abilities owing to random self-aggregation. However, external stimuli, such as the presence of liposomes or intracellular uptake, restored the fluorescence and singlet-oxygen-generation abilities of beta-glucan-complexed diazaporphyrins. Consequently, beta-glucan-complexed diazaporphyrins showed very high photodynamic activities toward HeLa cells.

    DOI: 10.1002/asia.201901582

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Books and Other Publications 【 display / non-display

  • 絵で見てわかるナノDDS 遺伝子医学MOOK別冊

    甲元 一也

    メディカルドゥ  2007 

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  • A novel triple helix consisting of one polynucleotide and two polysaccharide chainsMacromolecular Nano-Structured Material

    Edited by N. Ueyama, A. Harada 甲元一也( Role: Joint author)

    Macromolecular Nanostructured Material,  2004.11 

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    78, 281-296 (2004) Edited by N. Ueyama, A. Harada (Springer, 233 Spring Street, NewYork, NY 10013, USA)

  • Macromolecular Nanostructured Material

    甲元 一也

    Springer  2004 

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Review Papers (Misc) 【 display / non-display

  • 「受動的な学び」から「能動的な学び」へ 〜高大連携活動の展開と可能性〜 Invited

    甲元一也

    月刊「兵庫教育」   74 ( 3 )   4 - 7   2022.6

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    Authorship:Lead author, Corresponding author   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

  • Formation of beta-(1,3-1,6)-D-glucan-complexed [70]fullerene and its photodynamic activity towards macrophages (vol 15, pg 1990, 2017) International journal

    Atsushi Ikeda, Motofusa Akiyama, Kouta Sugikawa, Kazuya Koumoto, Yuta Kagoshima, Jiawei Li, Toshio Suzuki, Takeshi Nagasaki

    ORGANIC & BIOMOLECULAR CHEMISTRY   15 ( 45 )   9734 - 9734   2017.12

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    Publisher:ROYAL SOC CHEMISTRY  

    Correction for 'Formation of β-(1,3-1,6)-d-glucan-complexed [70]fullerene and its photodynamic activity towards macrophages' by Atsushi Ikeda et al., Org. Biomol. Chem., 2017, 15, 1990-1997.

    DOI: 10.1039/c7ob90179h

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  • 化学修飾技術を利用した乳酸菌模倣粒子による免疫賦活 Invited

    甲元一也、長濱宏治、松井淳

    バイオインダストリー   32 ( 9 )   42 - 47   2015.9

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    Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

  • 代謝産物アナログによる酵素活性化・安定化

    高木琴味、甲元一也

    月刊バイオインダストリー   30 ( 8 )   43 - 50   2013.8

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    Authorship:Lead author   Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)   Publisher:CMC出版  

  • Cellular zwitterionic metabolite analogs simultaneously enhance reaction rate, thermostability, salt tolerance, and substrate specificity of alpha-glucosidase International journal

    Eisuke Deguchi, Kazuya Koumoto

    BIOORGANIC & MEDICINAL CHEMISTRY   19 ( 10 )   3128 - 3134   2011.5

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    Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    We investigated the structural effects of metabolite analogs derived from a naturally-occurring zwitterionic metabolite, glycine betaine, on the activity of several hydrolases. The initial velocities of the hydrolases were enhanced by the addition of the solutes into the buffer solution. Based on a detailed study using alpha-glucosidases, the acceleration efficiency of the enzymatic activity was strongly induced by solutes possessing bulky and aliphatic ammonium cations, indicating that enhancement of activity by the solutes depended on their chemical structures. Kinetic analysis revealed that the acceleration of the hydrolysis reaction was related to both the decrement of K-m and increment of V-max values. Furthermore, the addition of the metabolite analogs enhanced not only the rate constant but also the thermostability, salt tolerance, and substrate specificity of alpha-glucosidase simultaneously through the reduction of conformational perturbation of the enzyme. (C) 2011 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.bmc.2011.04.003

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Presentations 【 display / non-display

  • β-1,3-1,6-グルカンおよびその包接複合体ナノ粒子からなる易水溶性粉末の調製

    甲元一也・児玉歩夢・土居菜々子・畦崎梨花・森田志穂・杉野遥香

    第71回高分子討論会  (北海道大学札幌キャンパス)  2022.9 

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    Event date: 2022.9

    Country:Japan  

  • β-1,3-1,6-グルカンナノ粒子を利用した抗酸化物質のデリバリーシステムの開発

    甲元一也・土居菜々子

    第71回高分子討論会  (北海道大学札幌キャンパス)  2022.9 

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    Event date: 2022.9

    Country:Japan  

  • 変性β-1,3-1,6-グルカンナノ粒子のキャビティに包接されるゲスト分子の構造要件の探索

    西田和史、土居菜々子、甲元一也

    第19回ホスト-ゲスト・超分子化学シンポジウム  (岡山大学津島キャンパス)  2022.6 

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    Event date: 2022.6

  • オリゴ糖、多糖を基体とするホストに包接された抗酸化物質の抗酸化活性の比較

    土居菜々子、甲元一也

    第67回高分子研究発表会(神戸)  (オンライン)  2021.7  高分子学会関西支部

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    Event date: 2021.7

  • オキシダーゼ/ペルオキシダーゼ系に及ぼすベタイン型添加剤の混合効果の解析

    上甲育実、甲元一也

    第18回ホスト-ゲスト・超分子化学シンポジウム  (オンライン)  高分子学会関西支部

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    Event date: 2021.6

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Industrial property rights 【 display / non-display

  • 有機化学反応に使用される水系反応溶媒

    甲元一也、北村武大、山本真史、郷田慎

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    Application no:特願2021-005314

    Announcement no:特開2022-109793

  • 水性二相分離液、及び当該水性二相分離液を用いた有機物の分離方法

    甲元一也、北村武大、山本真史、郷田慎

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    Application no:特願2021-005313

    Announcement no:特開2022-109792

  • デバイス及びその製造方法

    甲元一也、橋本賀之、北村武大、森田祐子、山本真史

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    Application no:特願2020-37096

    Announcement no:特開2021-136916

  • 基質溶液

    甲元一也、塩江一磨、木全伸介

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    Application no:特願2018-034728

    Announcement no:特開2019-146541

  • β-1,3-1,6-グルカン粉末、グルカン含有組成物、β-1,3-1,6-グルカン粉末の製造方法、包接複合体、包接複合体の製造方法およびゲスト分子の回収方法

    甲元一也、北原義孝

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    Application no:PCT/JP20

    Publication no:WO2019/073989 A1

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Academic Awards Received 【 display / non-display

  • バイオテックグランプリ2021最優秀賞

    2021.9   株式会社リバネス  

    B-Lab

  • バイオテックグランプリ2021AQI賞

    2021.9   株式会社リバネス  

    B-Lab

Grant-in-Aid for Scientific Research 【 display / non-display

  • 代謝産物の生体高分子の安定化、活性化に関する作用機構の解明

    2010.4 - 2012.3

    JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists(B)

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    細胞がストレスを受けた際に細胞内に産生する代謝産物群は、細胞機能を内的、外的に制御することができる新規バイオマテリアルとして注目を集めている。申請者のグループでは、有機合成的手法で構造改変したライブラリーを用い、代謝物質の化学構造が細胞機能に及ぼす影響について検討を行ってきた。その結果、生体高分子の安定性や活性は、代謝産物の化学構造に強く影響されることを明らかとしてきた。本申請では、適合溶質の生体高分子へ及ぼす作用機構を解明する。得られた知見は、細胞工学や酵素工学の分野に広く応用できると考えている。

  • 合成的アプローチによる適合溶質の機能解明とその生物学的応用

    2008.4 - 2010.3

    JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists(B)

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    有機合成的手法により細胞内の機能が未だ不明な適合溶質ライブラリーを構築し、その化学構造と細胞内機能の相関を明らかとする。

  • 多糖・無機ハイブリッドシステムを利用した遺伝子診断システムの開発

    2002.4 - 2004.3

    JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows

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    核酸と高分子複合体を形成する天然多糖シゾフィランの基盤への固定化技術の開発とそれによる核酸の検出システムの開発

Other External funds procured 【 display / non-display

  • 酵素機能を低濃度で効率よく向上させる新規酵素活性化剤の開発

    2011.4 - 2012.3

    その他財団等  平成22年度野口遵研究助成金

Preferred joint research theme 【 display / non-display

  • オリゴ糖、多糖の化学合成、化学修飾に関する研究

  • 酵素の活性化に関する研究

 

Committee Memberships 【 display / non-display

  • 2010.4   有機合成化学協会(国内)  会員、関西支部幹事

  • 2010   有機合成化学協会  幹事

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Social Activities 【 display / non-display

  • 微生物が創り出す天然多糖を加工してカプセルを作る 〜多糖ナノカプセルが変える未来の食品、化粧品、医薬品〜

    Role(s): Lecturer

    兵庫県立豊岡高校  2022.9

  • 講演「仮設の立て方に重点をおいたリサーチプランの立案」

    Role(s): Lecturer, Advisor

    兵庫「咲いテク」委員会  2022.8

  • 実験講義「研究体験2022 〜機能性色素を作る、調べる〜」

    Role(s): Lecturer

    兵庫県立豊岡高校  2022.8

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    Audience: High school students

  • 講義「研究体験2022 〜機能性色素を作る、調べる〜(事前学習)」

    Role(s): Lecturer

    兵庫県立豊岡高校  2022.7

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    Audience: High school students

  • 講義「課題探究テーマの設定法」

    Role(s): Lecturer

    兵庫県立神戸高校  2022.6

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    Audience: High school students

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