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Professor |
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Research Field |
germ cell biology, germ cell development, Genetics, Epigenetics, Life Science / Molecular biology |
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Graduating School 【 display / non-display 】
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Shizuoka University Faculty of Science Graduated
- 1990.3
Graduate School 【 display / non-display 】
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Hiroshima University Graduate School, Division of Natural Science Doctor's Course Completed
- 1995.3
Campus Career 【 display / non-display 】
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KONAN UNIVERSITY Faculty of Science and Engineering Faculty of Science and Engineering Department of Biology Professor
2017.4
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KONAN UNIVERSITY Faculty of Science and Engineering Associate Professor
2012.4 - 2017.3
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KONAN UNIVERSITY Faculty of Science and Engineering Lecturer
2007.4 - 2012.3
External Career 【 display / non-display 】
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岡崎共同研究機構基礎生物学研究所
2001.4 - 2007.3
Country：Japan
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日本学術振興会
2000.4 - 2001.3
Country：Japan
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筑波大学生物科学系 リサーチ・アソシエイト
1999.4 - 2000.3
Country：Japan
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筑波大学先端学際領域研究センター
1996.4 - 1999.3
Country：Japan
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国際科学振興財団
1995.4 - 1996.3
Country：Japan
Papers 【 display / non-display 】
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A truncated form of a transcription factor Mamo activates vasa in Drosophila embryos. Reviewed
Shoichi Nakamura, Seiji Hira, Masato Fujiwara, Nasa Miyagata, Takuma Tsuji, Akane Kondo, Hiroshi Kimura, Yuko Shinozuka, Makoto Hayashi, Satoru Kobayashi, Masanori Mukai
Communications biology 2019.11
Joint Work
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H3K36 Trimethylation-Mediated Epigenetic Regulation is Activated by Bam and Promotes Germ Cell Differentiation During Early Oogenesis in Drosophila Reviewed
Mukai M, Hira S, Nakamura K, Nakamura S, Kimura H, Sato M, Kobayashi S.
Biol Open. 2015
Joint Work
Authorship：Lead author
ヒストン修飾H3K36me3が生殖幹細胞の分化制御に関与することを明らかにした。
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Innexin2 gap junctions in somatic support cells are required for cyst formation and for egg chamber formation in Drosophila Reviewed
M. Mukai, H. Kato, S. Hira, K. Nakamura, H. Kita, S. Kobayashi
Mech. Dev. 128 510 - 523 2011.10
Joint Work
Authorship：Lead author
Germ cells require intimate associations with surrounding somatic cells during gametogenesis. During oogenesis, gap junctions mediate communication between germ cells and somatic support cells. However, the molecular mechanisms by which gap junctions regulate the developmental processes during oogenesis are poorly understood. We have identified a female sterile allele of innexin2 (inx2), which encodes a gap junction protein in Drosophila. In females bearing this inx2 allele, cyst formation and egg chamber formation are impaired. In wild-type germaria, Inx2 is strongly expressed in escort cells and follicle cells, both of which make close contact with germline cells. We show that inx2 function in germarial somatic cells is required for the survival of early germ cells and promotes cyst formation, probably downstream of EGFR pathway, and that inx2 function in follicle cells promotes egg chamber formation through the regulation of DE-cadherin and Bazooka (Baz) at the boundary between germ cells and follicle cells. Furthermore, genetic experiments demonstrate that inx2 interacts with the zero population growth (zpg) gene, which encodes a germline-specific gap junction protein. These results indicate a multifunctional role for Inx2 gap junctions in somatic support cells in the regulation of early germ cell survival, cyst formation and egg chamber formation. Inx2 junctions may mediate the transfer of nutrients and signal molecules between germ cells and somatic support cells, as well as play a role in the regulation of cell adhesion.
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Genetically encoded system to track histone acetylation in vivo Reviewed
Y. Sato, M. Mukai, J. Ueda, M. Muraki, T. J. Stasevich, N. Horikoshi, T. Kujirai, H. Kita, T. Kimura, S. Hira, Y. Okada, Y. Hayashi-Takanaka, C. Obuse, H. Kurumizaka, A. Kawahara, K. Yamagata, N. Nozaki, and H. Kimura
Scientific Reports 2013
Joint Work
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Binding of Drosophila maternal Mamo protein to chromatin and specific DNA sequences
S. Hira, T. Okamoto, M. Fujiwara, H. Kita, S. Kobayashi and M. Mukai
Biochem. Biophys Res. Commun. 2013
Joint Work
Review Papers (Misc) 【 display / non-display 】
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未来社会工学試論 （１）
栗田宣義、向 正則
甲南大学紀要 文学編 173 85 - 89 2023.3
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ショウジョウバエの母性因子SVA53による減数分裂課程の染色体構造制御（共著）
向 正則、小林 悟
生物の科学 遺伝 ( 21 ) 47 - 51 2007.3
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：（株）エヌ・ティー・エス
ショウジョウバエの母性因子SVA53による減数分裂課程の染色体構造制御
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Maternal nanos and Pumilio regulate zygotic vasa expression autonomously in the germ-line progenitors of Drosophila melanogaster embryos. （共著）
Mukai Masanori
Develop. Growth Differ. 43 ( 5 ) 545 - 552 2001
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Induction of indora expression in pole cells by the mesoderm is required for female germ-line development in Drosophila melanogaster
M Mukai, M Kashikawa, S Kobayashi
DEVELOPMENT 126 ( 5 ) 1023 - 1029 1999.3
Publisher：COMPANY OF BIOLOGISTS LTD
In many animal groups, the interaction between germ and somatic line is required for germ-line development. In Drosophila, the germ-line precursors (pole cells) formed at the posterior tip of the embryos migrate toward the mesodermal layer where they adhere to the dorsolateral mesoderm, which ensheaths the pole cells to form the embryonic gonads. These mesodermal cells may control the expression of genes that function in pole cells for their development into germ cells. However, such downstream genes have not been isolated, In this study, we identify a novel transcript, indora (idr), which is expressed only in pole cells within the gonads. Reduction of idr transcripts by an antisense idr expression caused the failure of pole cells to produce functional germ cells in females. Furthermore, me demonstrate that idr expression depends on the presence of the dorsolateral mesoderm, but it does not necessarily require its specification as the gonadal mesoderm, Our findings suggest that the induction of inr in pole cells by the mesodermal cells is required for germ-line development.
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Localization of mitochondorial large ribosomal RNA in germ plasm of Xenopus embryos. (共著)
Mukai Masanori
Current Biology 8 ( 20 ) 1117 - 1120 1998
Presentations 【 display / non-display 】
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ショウジョウバエ卵形成過程における熱ストレス誘導性オートファジーの性質
田中 彩葉、 奥村勇斗、青木友哉、兒山 萌華、向 正則
日本動物学会第93回大会 2022.9
Event date： 2022.9
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ショウジョウバエ卵巣における熱ストレス誘導性の卵室崩壊の解析
ショウジョウバエ卵巣における熱ストレス誘導性の卵室崩壊の解析
日本動物学会第93回大会 2022.9
Event date： 2022.9
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ショウジョウバエ卵巣における温熱耐性獲得機構の解析
三上 亮、奥村勇斗、青木友、田中 彩葉 、豊田 美香、向 正則
日本動物学会第93回大会 2022.9
Event date： 2022.9
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ショウジョウバエHeterochromatin protein 6 による初期胚の熱耐性獲得
奥村勇斗、青木友哉、田中 彩葉 、豊田 美香、向 正則
日本動物学会第93回大会 2022.9
Event date： 2022.9
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ショウジョウバエ卵巣における基本転写因パラログTRF2による熱ストレス応答の制御
田中 拓瑛、田中 拓瑛、三上 亮、田中 彩葉、青木友哉、奥村勇斗、向 正則
日本動物学会第93回大会 2022.9
Event date： 2022.9
Grant-in-Aid for Scientific Research 【 display / non-display 】
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ショウジョウバエ基本転写因子パラログTRF2による生殖細胞の維持機構の解析
2021.4 - 2023.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(C)
向 正則
生殖細胞の生と死のバランスが、種の存続に重要である。しかし、この制御に関わる分子機構とゲノムDNAの品質を脅かす環境ストレスとの関 係には不明な点が多い。本研究では、紫外線 (UV)と熱ストレスに注目し、これらのストレスがショウジョウバエの生殖細胞の形成機構に与え る影響を解析する。ストレス耐性やストレスからの回復に関わる遺伝子の機能解析を試みる。これまでに生殖細胞中の転写の調節に関わること が知られているTRF2やストレス応答に関わることが報告されている転写調節因子p53、さらにこれらの遺伝子と相互作用する遺伝子を中心に機 能解析を行い、生殖細胞の維持、品質管理に関わる分子機構の解析を行う。
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世代を超えたエピジェネティックな情報伝達に関わるヒストン修飾の探索
2016.4 - 2019.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(C)
ショウジョウバエを材料として、親世代の栄養条件が子世代の発生に与える影響を調べる。さらに、この世代間の情報伝達に関わる分子機構を生殖細胞に注目して解析する。
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ショウジョウバエの減数分裂を制御する母性因子複合体の解析
2009.4 - 2011.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(C)
ショウジョウバエの減数分裂を制御する母性因子複合体の解析
Social Activities 【 display / non-display 】
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出張講義 舞子高校
Role(s)： Appearance
2018.7
Audience： High school students
「私たちの体と遺伝子 ショウジョウバエ研究からわかること」
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出張講義 舞子高校
Role(s)： Appearance
2017.12
Audience： High school students
「私たちの体と遺伝子 ショウジョウバエ研究からわかること」