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Position |
Associate Professor |
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Research Field |
Life Science / Functional biochemistry, Nanotechnology/Materials / Bio chemistry |
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External Link |
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Graduating School 【 display / non-display 】
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Konan University Faculty of Science Graduated
- 2003.3
Graduate School 【 display / non-display 】
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Konan University Graduate School, Division of Science and Technology Doctor's Course Completed
- 2008.3
Campus Career 【 display / non-display 】
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KONAN UNIVERSITY Frontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry Associate Professor
2020.4
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KONAN UNIVERSITY Frontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry Lecturer
2016.4 - 2020.3
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KONAN UNIVERSITY Frontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry Assistant Professor
2010.7 - 2016.3
External Career 【 display / non-display 】
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株式会社ファイン
2008.4 - 2009.2
Country：Japan
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米国イリノイ大学
2008.4 - 2008.6
Country：United States
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日本学術振興会
2005.4 - 2008.3
Country：Japan
Professional Memberships 【 display / non-display 】
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Japan Society of Nucleic Acids Chemistry
2019
Research Career 【 display / non-display 】
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Development of new DNA materials using ionic liquids
(not selected)
Project Year： 2010.1 -
Papers 【 display / non-display 】
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Imperfect G-quadruplex as an emerging candidate for transcriptional regulation Reviewed International journal
S. Sarkar, H. Tateishi-Karimata, T. Ohyama, N. Sugimoto
Nucleic Acids Res. 53 ( 5 ) in press 2025.3
Authorship：Corresponding author
G-quadruplexes (G4s) with continuous G-tracts are well-established regulators of gene expression and important therapeutic targets for various diseases. However, bioinformatics analyses have identified G4-like sequences containing interrupted G-tracts, incorporating non-G nucleotides as bulges (buG4s). Our findings show that the stability of buG4s is significantly influenced by the bulge position and size within the G-tract, with bulges at the 5' end exhibiting the highest stability. Moreover, a molecular crowding condition inducing by poly (ethylene glycol), providing a suitable intracellular environment, stabilizes buG4s, especially those with longer bulges, making their formation more pronounced. A transcription assay performed under crowding conditions revealed that the transcription arrested efficiency by buG4s is affected not only by stability but also by the position and size of the bulge. Based on these findings, we propose a model for the preliminary screening of buG4 sequences according to their stability, distinguishing functional sequences capable of transcriptional arrest (ΔG°37 ≤ -3.3 kcal·mol-1) from nonfunctional sequences (ΔG°37 > -3.3 kcal·mol-1). This provides valuable insight into estimating the efficiency of target buG4 sequences in either arresting or facilitating transcription, presenting a novel approach and emphasizing buG4s as emerging therapeutic targets.
DOI： 10.1093/nar/gkaf164
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Controlling the local conformation of RNA G-quadruplex results in reduced RNA/peptide cytotoxic accumulation associated with C9orf72 ALS/FTD Reviewed International journal
S. Matsumoto, H. Tateishi-Karimata, T. Ohyama, N. Sugimoto
Small Methods 9 in press 2025.3
Authorship：Corresponding author
Repeat expansion of d(G4C2) in the noncoding region of the C9orf72 gene contributes to neurodegenerative diseases. The repeat expansion transcript r(G4C2) induces RNA/peptide accumulation, which, in turn, induces cytotoxicity and accelerates the development of neurodegenerative diseases. Such cytotoxic accumulation is triggered by peptide aggregation. Here, a technique is developed to prevent accumulation by regulating RNA interactions, assuming that RNA structure is important for peptide interactions. A screening method is used to identify compounds that suppress RNA accumulation of r(G4C2) repeats. The four compounds are identified with wide π-planes containing hydroxyl, methoxy, and cyclic ether groups that suppressed RNA accumulation. Interestingly, these compounds also suppressed RNA/peptide accumulation in neuroblastoma cells, indicating that RNA accumulation is a key regulator of RNA/peptide cytotoxic aggregate formation. In vitro and in silico physicochemical analyses reveal that these compounds bind to the loop region of the G-quadruplex via hydrogen bonds or CH-π interactions, resulting in an altered loop conformation. Importantly, these conformational changes inhibited RNA G-quadruplex associations. These results show that conformational changes are promising for controlling the interactions between G-quadruplexes and further RNA accumulation. These findings may be useful in the development of therapeutic strategies for the treatment of neurodegenerative diseases.
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Development of a Pseudocellular System to Quantify Specific Interactions Determining the G-Quadruplex Function in Cells International journal
Hisae Tateishi-Karimata, Keiko Kawauchi, Shuntaro Takahashi, Naoki Sugimoto
Journal of the American Chemical Society 146 ( 12 ) 8005 - 8015 2024.3
Publisher：American Chemical Society (ACS)
Intracellular chemical microenvironments, including ion concentrations and molecular crowding, play pivotal roles in cell behaviors, such as proliferation, differentiation, and cell death via regulation of gene expression. However, there is no method for quantitative analysis of intracellular environments due to their complexity. Here, we have developed a system for highlighting the environment inside of the cell (SHELL). SHELL is a pseudocellular system, wherein small molecules are removed from the cell and a crowded intracellular environment is maintained. SHELL offers two prominent advantages: (1) It allows for precise quantitative biochemical analysis of a specific factor, and (2) it enables the study of any cell, thereby facilitating the study of target molecule effects in various cellular environments. Here, we used SHELL to study G-quadruplex formation, an event that implicated cancer. We show that G-quadruplexes are more stable in SHELL compared with in vitro conditions. Although malignant transformation perturbs cellular K+ concentrations, environments in SHELL act as buffers against G-quadruplex destabilization at lower K+ concentrations. Notably, the buffering effect was most pronounced in SHELL derived from nonaggressive cancer cells. Stable G-quadruplexes form due to the binding of the G-quadruplex with K+ in different cancer cells. Furthermore, the observed pattern of G-quadruplex-induced transcriptional inhibition in SHELL is consistent with that in living cells at different cancer stages. Our results indicate that ion binding to G-quadruplexes regulates gene expression during pathogenesis.
DOI： 10.1021/jacs.3c11160
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Choline Dihydrogen Phosphate Destabilizes G-Quadruplexes and Enhances Transcription Efficiency In Vitro and in Cells. International journal
Hisae Tateishi-Karimata, Naoki Sugimoto
ACS omega 9 ( 5 ) 5675 - 5682 2024.2
G-quadruplexes in disease-related genes are associated with various biological processes and regulate disease progression. Although methods involving ligands and other techniques are available to stabilize G-quadruplexes, approaches for destabilizing G-quadruplexes remain limited. Here, we evaluated whether G-quadruplexes can be destabilized using choline dihydrogen phosphate (choline dhp), a highly biocompatible hydrated ionic liquid. Circular dichroism spectral measurements at increasing temperatures revealed that choline dhp destabilized G-quadruplexes more effectively than did KCl-containing solutions. Thermodynamic analysis indicated that destabilization occurred via an entropic contribution, suggesting that choline ions did not coordinate with the G-quartets, because of their large radii. Subsequently, plasmid DNAs containing G-quadruplexes were constructed, and transcription reactions were performed in nuclear extracts from living cells. G-quadruplexes repressed transcription, whereas the addition of choline dhp increased transcription. Although ionic liquids often inactivate biomolecules, choline dhp can be used to culture various cells. Furthermore, the transcription of template DNA containing the G-quadruplex was greatly enhanced in living MDA-MD-231 cells (aggressive human breast cancer cells) cultured with choline dhp. Our results show that choline dhp destabilizes G-quadruplexes in cells, indicating that choline dhp can regulate gene expression. Thus, choline dhp may be useful for regulating target disease-related genes.
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In-Cell Stability Prediction of RNA/DNA Hybrid Duplexes for Designing Oligonucleotides Aimed at Therapeutics. International journal
Dipanwita Banerjee, Hisae Tateishi-Karimata, Maria Toplishek, Tatsuya Ohyama, Saptarshi Ghosh, Shuntaro Takahashi, Marko Trajkovski, Janez Plavec, Naoki Sugimoto
Journal of the American Chemical Society 145 ( 43 ) 23503 - 23518 2023.11
In cells, the formation of RNA/DNA hybrid duplexes regulates gene expression and modification. The environment inside cellular organelles is heterogeneously crowded with high concentrations of biomolecules that affect the structure and stability of RNA/DNA hybrid duplexes. However, the detailed environmental effects remain unclear. Therefore, the mechanistic details of the effect of such molecular crowding were investigated at the molecular level by using thermodynamic and nuclear magnetic resonance analyses, revealing structure-dependent destabilization of the duplexes under crowded conditions. The transition from B- to A-like hybrid duplexes due to a change in conformation of the DNA strand guided by purine-pyrimidine asymmetry significantly increased the hydration number, which resulted in greater destabilization by the addition of cosolutes. By quantifying the individual contributions of environmental factors and the bulk structure of the duplex, we developed a set of parameters that predict the stability of hybrid duplexes with conformational dissimilarities under diverse crowding conditions. A comparison of the effects of environmental conditions in living cells and in vitro crowded solutions on hybrid duplex formation using the Förster resonance energy transfer technique established the applicability of our parameters to living cells. Moreover, our derived parameters can be used to estimate the efficiency of transcriptional inhibition, genome editing, and silencing techniques in cells. This supports the usefulness of our parameters for the visualization of cellular mechanisms of gene expression and the development of nucleic acid-based therapeutics targeting different cells.
DOI： 10.1021/jacs.3c06706
Books and Other Publications 【 display / non-display 】
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月刊化学「化学者が思い描く未来の社会」
建石 寿枝（ Role： Joint author , ⑥非二重らせん核酸により生命の機能を制御する ―生物種を超えた統一ルール）
2021.12
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CSJカレントレビュー「進化を続ける核酸化学―ゲノム編集，非二重らせん，核酸医薬」
神谷 真子, 建石 寿枝, 永 次史, 山吉 麻子, 杉本 直己（ Role： Joint author , 第1章フロントランナーに聞く「令和の時代も進化を続ける核酸化学」）
2021.10
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相分離生物学の全貌（現代化学増刊46)
建石寿枝, 杉本直己（ Role： Joint author , 第Ⅳ部 生物学的相分離の理論）
東京化学同人 2020.11 （ ISBN:9784807913466 ）
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生体分子化学―基礎から応用まで (エキスパート応用化学テキストシリーズ)
杉本 直己, 内藤 昌信, 橋詰 峰雄, 高橋 俊太郎, 田中 直毅, 建石 寿枝, 遠藤 玉樹, 津本 浩平, 長門石 曉, 松原 輝彦, 上田 実, 朝山 章一郎
講談社 2017.1 （ ISBN:406156806X ）
Review Papers (Misc) 【 display / non-display 】
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Mechanisms of gene expression regulated by non-canonical structures of nucleic acids
Tateishi-Karimata Hisae
Journal of The Society of Japanese Women Scientists 25 37 - 42 2025.1
Publisher：The Society of Japanese Women Scientists
The structure of nucleic acids is a double helix structure (duplex) consisting of Watson-Crick base pairs. Recently, it has become clear that nucleic acids can also form non-canonical structures such as triplexes and Gquadruplexes depending on surrounding environments. Interestingly, nucleic acid sequences that can form noncanonical structures are often found on genes associated with cancer and neurodegenerative diseases. Moreover, it has been reported that the formation of non-canonical structures can induce mutations in gene expression, such as transcription and translation. The role of non-canonical structures in the onset and progression of disease has therefore attracted attention, and research to clarify the correlation between the formation of non-canonical structures and changes in disease gene expression is being conducted worldwide. These findings may help to elucidate the molecular mechanisms of disease onset and progression. This review outlines the non-canonical structures formed on disease-related genes (particularly oncogenes) and the effects of nucleic acid structures on gene expression.
DOI： 10.5939/sjws.250007
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TATEISHI-KARIMATA Hisae
POLYMERS 73 ( 9 ) 464 - 464 2024
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神経変性疾患に関わる核酸の周辺環境に依存した構造解析とその制御
建石寿枝, 建石寿枝, 杉本直己
日本分子生物学会年会プログラム・要旨集(Web) 47th 2024
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Creation of artificial viral capsid encapsulated quadruplex DNA
石井楽乃, 稲葉央, 遠藤玉樹, 建石寿枝, 杉本直己, 松浦和則
日本化学会春季年会講演予稿集(Web) 104th 2024
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核酸の構造及び安定性の制御に基づく新規の脳疾患抑制法の開発 [2020（令和2）年度分]
杉本 直己, 遠藤 玉樹, 高橋 俊太郎, 建石 寿枝
甲南学園平生太郎基金科学研究報告書 = The Hirao Taro Foundation of KONAN GAKUEN for Academic Research Report 14 87 - 126 2023.3
Presentations 【 display / non-display 】
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「核酸の姿と病気の関わり」 Invited
建石 寿枝
日本薬学会関西支部主催：市民公開講座「環境によって変わる核酸の姿と病気：ヒトからウイルスまでを標的とした創薬を目指して」 2022.12
Event date： 2022.12
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生物種を超えた多元応答機構の解明を目指した核酸構造の解析
建石寿枝
第95回日本生化学会大会 シンポジム「非二重らせん核酸の多元機能」 2022.11
Event date： 2022.11
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Quantitative analysis for G-quadruplex and i-motif formations in malignant cancers
Hisae Tateishi-Karimata, Naoki Sugimoto
2022.11
Event date： 2022.11
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細胞夾雑模倣系の構築と細胞内活性分子の設計指針
建石寿枝, 三好大輔, 杉本直己
分子夾雑の生命化学成果とりまとめ公開シンポジム 2022.9
Event date： 2022.9
Industrial property rights 【 display / non-display 】
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核酸の立体構造を制御する方法及びその用途、並びに、細胞内分子クラウディング環境を再現するための組成物
建石 寿枝、高橋 俊太郎、川内 敬子、杉本 直己
Application no：2022-189538
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核酸の立体構造を制御する方法及びその用途、並びに、細胞内分子クラウディング環境を再現するための組成物
建石 寿枝, 川内 敬子, 高橋 俊太郎, 杉本 直己
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核酸鎖の四重螺旋構造の形成を可能にするデオキシヌクレオシド誘導体
杉本 直己, 建石 寿枝, 金原 数, 村岡 貴博
Application no：特願2015-095059
Announcement no：特開2016-210719
Patent/Registration no：特許第6802964号
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核酸塩基対の安定性を塩基対選択的に変える方法
建石 寿枝、 杉本 直己
Application no：特願2011-117381
Country of applicant：Domestic
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佐々木 義晴, 西田 尚広, 瀧上 忠一, 建石 寿枝
Academic Awards Received 【 display / non-display 】
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The Society of Japan Women Scientists for honorable mention award (2024)
2024.6
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2019年度甲南大学教員功績表彰者
2020.3 甲南大学
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日本化学会第94春季年会 優秀講演賞（学術）
2014.3 日本化学会
建石寿枝
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5th International Symposium on Nucleic Acids Chemistry ポスター優秀賞 (Nucleic Acids Research賞)
2007.11 ａ
狩俣 寿枝 （建石の旧姓 ）
Grant-in-Aid for Scientific Research 【 display / non-display 】
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Quantitative prediction of nucleic acid structures and functions affected by spaciotemporal environmental factors in cells
2022.4 - 2027.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(S)
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Elucidation of the mechanism for dimensional response genome using intracellular environmental evaluation systems and development of dimensional response genome bank
2021.8 - 2024.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid (B) (Tentative)
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Elucidation of the mechanism for dimensional response genome
2021.8 - 2024.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid (B) (Tentative)
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2021.8 - 2024.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid (B) (Tentative)
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Do ion channels control the formation of non-canonical nucleic acids and gene expression?
2020.7 - 2022.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
Sugimoto Naoki
The formation of nucleic acids of non-canonical structures induces mutations in biological reactions such as replication, transcription, and translation. In diseased cells, the molecular environments, especially ionic environments, are very different from that of normal cells due to overexpression (or inactivation) of disease-specific ion channels. These environmental differences are thought to affect the formation of non-canonical structures. In this study, we analyzed nucleic acid structures in the environments that mimic the molecular environment in diseased cells using physicochemical methods. As results, we clarified the mechanisms of stability changes of the non-canonical structures by intracellular environmental factors. Furthermore, we developed techniques to control gene expression by ion-nucleic acid interactions using small molecules and modified nucleic acids.
Other External funds procured 【 display / non-display 】
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極限環境により誘起されるDNA特殊構造を活用したDNAスイッチの開発
2014.4 - 2015.3
公益財団法人ひょうご科学技術協会 公益財団法人ひょうご科学技術協会 平成26年度学術研究助成
Preferred joint research theme 【 display / non-display 】
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機能性核酸および酵素の活性を溶液環境で制御することを活用したナノマテリアル（センサーなど）の開発
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細胞内で活用できる機能性核酸の開発
Social Activities 【 display / non-display 】
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なでしこscientistトーク
Role(s)： Appearance, Presenter
甲南大学 先端生命工学研究所 なでしこscientistトーク 甲南大学先端生命工学研究所 2014.6
Audience： High school students, College students, Graduate students, Teachers, Guardians, Researchesrs, General
最先端の科学技術について、女性研究者がわかりやすく解説する講演会。
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第10回なでしこscientistトーク「新型コロナウィルス感染症に挑む（COVID-19）」
Role(s)： Lecturer
甲南大学先端生命工学研究所 親和女子高等学校 2020.10
Academic Activities 【 display / non-display 】
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第95回日本生化学会大会のシンポジウム 2S09e 「非二重らせん核酸の多元機能」
Role(s)： Planning, management, etc., Panel moderator, session chair, etc.
日本生化学会 ・ 今西 未来（京都大学化学研究所)・建石 寿枝（甲南大学先端生命工学研究所(FIBER)) ・学術変革領域研究(B)「多元応答ゲノム」 （ 名古屋国際会議場 第9会場（222） ） 2022.11
Type：Competition, symposium, etc.
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B領域横断研究会（糖化学ノックイン・多元応答ゲノム）
Role(s)： Planning, management, etc., Panel moderator, session chair, etc.
学術変革領域（B）「糖化学ノックイン」「多元応答ゲノム」 （ グランフロント大阪北館 アクティブスタジオ ） 2022.10
Type：Competition, symposium, etc.
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ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～ＫＡＫＥＮＨＩ
Role(s)： Planning, management, etc., Panel moderator, session chair, etc.
甲南大学先端生命工学研究所 （ 甲南大学ポートアイランドキャンパス ） 2022.8
遺伝子を観て、新しい機能について学ぼう～mRNAワクチンやPCR検査のしくみ～
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ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～ＫＡＫＥＮＨＩ
Role(s)： Planning, management, etc., Panel moderator, session chair, etc.
甲南大学先端生命工学研究所 （ 甲南大学ポートアイランドキャンパス ） 2022.8
体験しよう PCR 検査！学ぼう遺伝子の仕組み！
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FIBER核酸化学ユニバース９
Role(s)： Panel moderator, session chair, etc.
甲南大学先端生命工学研究所 学術変革領域（B）「多元応答ゲノム」多元応答ゲノム領域推進センター （ オンライン ） 2022.2
Type：Competition, symposium, etc.
Qualification acquired 【 display / non-display 】
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High School Teacher Specialization License