写真a

TATEISHI Hisae

Position

Associate Professor

External Link

Graduating School 【 display / non-display

  • Konan University   Faculty of Science   Graduated

    - 2003.3

Graduate School 【 display / non-display

  • Konan University   Graduate School, Division of Science and Technology   Doctor's Course   Completed

    - 2008.3

Campus Career 【 display / non-display

  • KONAN UNIVERSITY   Frontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry   Associate Professor

    2020.4

  • KONAN UNIVERSITY   Frontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry   Lecturer

    2016.4 - 2020.3

  • KONAN UNIVERSITY   Frontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry   Assistant Professor

    2010.7 - 2016.3

External Career 【 display / non-display

  • 株式会社ファイン

    2008.4 - 2009.2

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    Country:Japan

  • 米国イリノイ大学

    2008.4 - 2008.6

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    Country:United States

  • 日本学術振興会

    2005.4 - 2008.3

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    Country:Japan

Professional Memberships 【 display / non-display

  • Japan Society of Nucleic Acids Chemistry

    2019

 

Research Career 【 display / non-display

  • Development of new DNA materials using ionic liquids

    (not selected)  

    Project Year: 2010.1  -   

Papers 【 display / non-display

  • Chemical biology of non-canonical structures of nucleic acids for therapeutic application Reviewed

    H. Tateishi-Karimata and N. Sugimoto

    Chem. Commun.   2020.2

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    Joint Work

  • Chemical Modulation of DNA Replication along G-Quadruplex Based on Topology-Dependent Ligand Binding.

    Shuntaro Takahashi, Anita Kotar, Hisae Tateishi-Karimata, Sudipta Bhowmik, Zi-Fu Wang, Ta-Chau Chang, Shinobu Sato, Shigeori Takenaka, Janez Plavec, Naoki Sugimoto

    Journal of the American Chemical Society   143 ( 40 )   16458 - 16469   2021.10

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    Joint Work

    Ligands that bind to and stabilize guanine-quadruplex (G4) structures to regulate DNA replication have therapeutic potential for cancer and neurodegenerative diseases. Because there are several G4 topologies, ligands that bind to their specific types may have the ability to preferentially regulate the replication of only certain genes. Here, we demonstrated that binding ligands stalled the replication of template DNA at G4, depending on different topologies. For example, naphthalene diimide derivatives bound to the G-quartet of G4 with an additional interaction between the ligand and the loop region of a hybrid G4 type from human telomeres, which efficiently repressed the replication of the G4. Thus, these inhibitory effects were not only stability-dependent but also topology-selective based on the manner in which G4 structures interacted with G4 ligands. Our original method, referred to as a quantitative study of topology-dependent replication (QSTR), was developed to evaluate correlations between replication rate and G4 stability. QSTR enabled the systematic categorization of ligands based on topology-dependent binding. It also demonstrated accuracy in determining quantitatively how G4 ligands control the intermediate state of replication and the kinetics of G4 unwinding. Hence, the QSTR index would facilitate the design of new drugs capable of controlling the topology-dependent regulation of gene expression.

    DOI: 10.1021/jacs.1c05468

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  • Roles of non-canonical structures of nucleic acids in cancer and neurodegenerative diseases.

    Hisae Tateishi-Karimata, Naoki Sugimoto

    Nucleic acids research   49 ( 14 )   7839 - 7855   2021.8

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    Joint Work

    Cancer and neurodegenerative diseases are caused by genetic and environmental factors. Expression of tumour suppressor genes is suppressed by mutations or epigenetic silencing, whereas for neurodegenerative disease-related genes, nucleic acid-based effects may be presented through loss of protein function due to erroneous protein sequences or gain of toxic function from extended repeat transcripts or toxic peptide production. These diseases are triggered by damaged genes and proteins due to lifestyle and exposure to radiation. Recent studies have indicated that transient, non-canonical structural changes in nucleic acids in response to the environment can regulate the expression of disease-related genes. Non-canonical structures are involved in many cellular functions, such as regulation of gene expression through transcription and translation, epigenetic regulation of chromatin, and DNA recombination. Transcripts generated from repeat sequences of neurodegenerative disease-related genes form non-canonical structures that are involved in protein transport and toxic aggregate formation. Intracellular phase separation promotes transcription and protein assembly, which are controlled by the nucleic acid structure and can influence cancer and neurodegenerative disease progression. These findings may aid in elucidating the underlying disease mechanisms. Here, we review the influence of non-canonical nucleic acid structures in disease-related genes on disease onset and progression.

    DOI: 10.1093/nar/gkab580

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  • Chemical Biology of Double Helical and Non-Double Helical Nucleic Acids: “To B or Not To B, That Is the Question”

    Naoki Sugimoto, Tamaki Endoh, Shuntaro Takahashi, Hisae Tateishi-Karimata

    Bulletin of the Chemical Society of Japan   94 ( 7 )   1970 - 1998   2021.7

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    Publisher:The Chemical Society of Japan  

    DOI: 10.1246/bcsj.20210131

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  • Effect of Molecular Crowding on the Stability of RNA G-Quadruplexes with Various Numbers of Quartets and Lengths of Loops.

    Saki Matsumoto, Hisae Tateishi-Karimata, Shuntaro Takahashi, Tatsuya Ohyama, Naoki Sugimoto

    Biochemistry   59 ( 28 )   2640 - 2649   2020.7

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    G-Quadruplexes are noncanonical structures formed by guanine-rich regions of not only DNA but also RNA. RNA G-quadruplexes are widely present in the transcriptome as mRNAs and noncoding RNAs and take part in various essential functions in cells. Furthermore, stable RNA G-quadruplexes control the extent of biological functions, such as mRNA translation and antigen presentation. To understand and regulate the functions controlled by RNA G-quadruplexes in cellular environments, which are molecularly crowded, we would be required to investigate the stability of G-quadruplexes in molecular crowding. Here, we systematically investigated the thermodynamic stability of RNA G-quadruplexes with different numbers of G-quartets and lengths of loops. The molecular crowding conditions of polyethylene glycol with an average molecular weight of 200 (PEG200) were found to stabilize RNA G-quadruplexes with three and four G-quartets, while G-quadruplexes with two G-quartets did not exhibit any stabilization upon addition of PEG200. On the other hand, no difference in stabilization by PEG200 was observed among the G-quadruplexes with different loop lengths. Thermodynamic analysis of the RNA G-quadruplexes revealed more appropriate motifs for identifying G-quadruplex-forming sequences. The informatics analysis with new motifs demonstrated that the distributions of G-quadruplexes in human noncoding RNAs differed depending on the number of G-quartets. Therefore, RNA G-quadruplexes with different numbers of G-quartets may play different roles in response to environmental changes in cells.

    DOI: 10.1021/acs.biochem.0c00346

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Books and Other Publications 【 display / non-display

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Review Papers (Misc) 【 display / non-display

  • The function of rDNA G-quadruplex aggregates formed by nucleolar stress

    杉本渉, 桑本慎, 建石寿枝, 岩根敦子, 岩根敦子, 岩根敦子, 高橋宏隆, 三好大輔, 川内敬子

    日本薬学会年会要旨集(Web)   141年会   29V05 - am10S   2021.3

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  • Nucleic Acids Chemistry beyond the Watson-Crick Double Helix (69): Analysis of interactions between RNA G-quadruplexes and dipeptide repeats related to neurodegenerative diseases

    大山達也, 建石寿枝, 田中成典, 杉本直己, 杉本直己

    日本化学会春季年会講演予稿集(Web)   101st   2021

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  • クラウディングの実験と理論 (相分離生物学の全貌) -- (生物学的相分離の理論)

    建石 寿枝, 杉本 直己

    現代化学 : 増刊   ( 46 )   195 - 200   2020.11

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    Publisher:東京化学同人  

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  • The mecanism of nucleolus disruption by inhibiting rRNA transcription

    杉本渉, 豊田駿, 建石寿枝, 杉本直己, 岩根敦子, 岩根敦子, 岩根敦子, 三好大輔, 川内敬子

    日本薬学会年会要旨集(CD-ROM)   140年会   26G - pm10S   2020.3

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  • Nucleic Acids Chemistry beyond the Watson-Crick Double Helix (63): Accumulation mechanism of GGGGCC repeated RNA G-quadruplex with dipeptide repeats using molecular simulations

    大山達也, 建石寿枝, 田中成典, 杉本直己, 杉本直己

    日本化学会春季年会講演予稿集(CD-ROM)   100th   2020

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Presentations 【 display / non-display

  • 神経変性疾患に関わるリピートRNAによる相分離機構の解析

    建石寿枝, Ye Teng, 大山達也, 田中成典, 杉本直己

    第14回バイオ関連化学シンポジウム  (オンライン開催) 

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    Event date: 2020.9

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  • Development of the prediction method for stability of RNA/DNA hybrids under a physiological condition

    BANERJEE Dipanwita, 建石寿枝, 大山達也, GHOSH Saptarshi, 遠藤玉樹, 高橋俊太郎, 杉本直己

    第14回バイオ関連化学シンポジウム  (オンライン開催) 

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    Event date: 2020.9

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  • Stability prediction of DNA duplexes available under diverse molecular crowding conditions

    GHOSH Saptarshi, 高橋俊太郎, 大山達也, 遠藤玉樹, 建石寿枝, 杉本直己

    第14回バイオ関連化学シンポジウム  (オンライン開催) 

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    Event date: 2020.9

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  • 異なるGカルテット数とループ長を有するRNAグアニン四重らせんの安定性への分子クラウディングの効果

    松本咲, 建石寿枝, 高橋俊太郎, 大山達也, 杉本直己

    第14回バイオ関連化学シンポジウム  (オンライン開催) 

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    Event date: 2020.9

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  • 脱ワトソン・クリックの核酸化学(63):分子シュミレーションによるGGGGCCリピートのRNA四重鎖とジペプチドリピートの集積メカニズムの解析

    大山達也, 建石寿枝, 田中成典, 杉本直己

    日本化学会第100回春季年会  (東京理科大学) 

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    Event date: 2020.3

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Industrial property rights 【 display / non-display

  • 核酸塩基対の安定性を塩基対選択的に変える方法

    建石 寿枝、 杉本 直己

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    Application no:特願2011-117381

    Country of applicant:Domestic  

  • 高品質化粧料

    佐々木 義晴, 西田 尚広, 瀧上 忠一, 建石 寿枝

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    Application no:特願2009-027695

    Announcement no:特開2010-180193

    J-GLOBAL

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Academic Awards Received 【 display / non-display

  • 日本化学会第94春季年会 優秀講演賞(学術)

    2014.3   日本化学会  

    建石寿枝

  • 資生堂サイエンスグラント

    2017.6   第10回女性研究者資生堂サイエンスグラント  

    建石 寿枝

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  • 5th International Symposium on Nucleic Acids Chemistry ポスター優秀賞 (Nucleic Acids Research賞)

    2007.11   a  

    狩俣 寿枝  (建石の旧姓 )

  • 日本化学会第87春季年会 学生講演賞

    2007.11   a  

    狩俣 寿枝  (建石の旧姓 )

  • 第18回生命分子化学研究会若手サマースクール ポスター賞

    2006.11   a  

    狩俣 寿枝  (建石の旧姓 )

Grant-in-Aid for Scientific Research 【 display / non-display

  • 細胞内の核酸構造の定量的解析を目指した疾患細胞モデル系の構築とその活用

    2020.4

    JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(B)

    建石寿枝

  • 細胞内の環境変化がDNAの構造及び機能に及ぼす影響の定量的解析

    2017.4 - 2020.3

    JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(C)

    建石寿枝

  • 極限環境におけるDNA相互作用を活用したDNA塩基対形成の制御

    2014.4 - 2017.3

    JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists(B)

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    DNAを薬剤や材料として活用する際、細胞内やチップ基板上の“超クラウディング環境”やイオン液体中の“超高塩濃度環境”などの“極限環境”によってDNA構造安定性は大きく影響を受ける。これまでこのような環境の効果は複雑であるため、環境効果を加味したDNA構造予測は困難であった。本研究では1)極限環境の物性変化(誘電率、水の活量変化など)に着目することによって、複雑な環境をシンプルに解析し、DNA構造安定性に及ぼす“極限環境”の影響をエネルギーレベルで理解することを試みる。さらに得られた知見を基に、2)DNAの構造形成の最も基本的な相互作用である塩基対形成能を極限環境によって制御し、DNAナノ構造体の構造制御を行う。

  • Construction of database of non-canonical structures of nucleic acids and its application for constructing artificial systems controlling biological reactions

    2012.4 - 2015.3

    JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(A)

    SUGIMOTO NAOKI, ENDOH Tamaki, TATEISHI Hisae

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    We quantitatively analyzed biophysical parameters of nucleic acids. To obtain the biophysical parameters, we constructed experimental systems considering molecular crowding conditions, which mimic intracellular molecular environment. We investigated effects of the biophysical parameters for non-canonical nucleic acid structures on gene expression. In addition, based on the chemical properties of the non-canonical structures, we designed artificial molecules that affect formation on the structures and their stabilities. We also demonstrated that transcription and translation reactions could be controlled by using the artificial molecules and systems. The results obtained in this study will provide new insights of biological significance of the non-canonical nucleic acid structures and novel technologies to regulate their functions in cells.

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  • Development of new DNA nano-switches based on the stabilization of A-T base pairs relative to G-C base pairs

    2012.4 - 2014.3

    JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for challenging Exploratory Research

    NAOKI Sugimoto, TATEISHI Hisae

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    We attempted the control of stabilities of Watson-Crick base pairs using a hydrated ionic liquid (IL) of choline dihydrogen phosphate (choline dhp), because hydrated ILs are green solvents suitable for a wide range of chemical reactions and may ensure long-term stability of biomolecules. Our quantitative analysis demonstrated that A-T base pairs are more stable than G-C base pairs in 4 M choline dhp. We also found that DNA triplex structures via the formation of Hoogsteen base pairs were significantly stabilized in the choline dhp solution compared to NaCl solutions. Thermodynamic analyses and molecular dynamics calculations revealed that the stabilization of A-T base pairs and Hoogsteen base pairs was due to specific binding of choline ions to DNA grooves. To take advantage of the stabilization of triplex formations to develop the sensing systems of double-stranded DNAs, we designed a DNA molecular beacon and found that the molecular beacon can specifically detect the target duplex.

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Other External funds procured 【 display / non-display

  • 極限環境により誘起されるDNA特殊構造を活用したDNAスイッチの開発

    2014.4 - 2015.3

    公益財団法人ひょうご科学技術協会  公益財団法人ひょうご科学技術協会 平成26年度学術研究助成

Preferred joint research theme 【 display / non-display

  • 機能性核酸および酵素の活性を溶液環境で制御することを活用したナノマテリアル(センサーなど)の開発

  • 細胞内で活用できる機能性核酸の開発

 

Social Activities 【 display / non-display

  • なでしこscientistトーク

    Role(s): Appearance, Presenter

    甲南大学 先端生命工学研究所  なでしこscientistトーク  甲南大学先端生命工学研究所  2014.6

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    Audience: High school students, College students, Graduate students, Teachers, Guardians, Researchesrs, General

    最先端の科学技術について、女性研究者がわかりやすく解説する講演会。

  • ひらめき☆ときめきサイエンス

    2011.8

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    生体分子を未来センサーとして使おう ~DNA で陽イオンを検出する~

  • NanoBioCollege2011社会人向け連続講座/FIBER研究成果報告

    2011.6

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    NanoBioCollege2011社会人向け連続講座/FIBER研究成果報告

  • NanoBioCollege2010社会人向け連続講座/FIBERから発信する最先端テクノロジー

    2010.11

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    社会人向けに最新のナノテクノロジーに関する講義を行った

  • ひらめき☆ときめきサイエンス

    2010.8

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    最新ノーベル賞堪能ツアー ―蛍光タンパク質と光るDNA実験―と題して中学生向けに実験講義を行った。本講義は、独立行政法人日本学術振興会の助成を受けたプログラムである。

 

Qualification acquired 【 display / non-display

  • High School Teacher Specialization License