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Position |
Associate Professor |
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Research Field |
Life Science / Functional biochemistry, Nanotechnology/Materials / Bio chemistry |
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External Link |
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Graduating School 【 display / non-display 】
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Konan University Faculty of Science Graduated
- 2003.3
Graduate School 【 display / non-display 】
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Konan University Graduate School, Division of Science and Technology Doctor's Course Completed
- 2008.3
Campus Career 【 display / non-display 】
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KONAN UNIVERSITY Graduate School of Frontiers of Innovative Research in Science and Techonology (Masters Degree Program) Graduate School of Frontiers of Innovative Research in Science and Technology (FIRST) Associate Professor
2025.4
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KONAN UNIVERSITY Frontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry Associate Professor
2020.4
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KONAN UNIVERSITY Frontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry Lecturer
2016.4 - 2020.3
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KONAN UNIVERSITY Frontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry Assistant Professor
2010.7 - 2016.3
External Career 【 display / non-display 】
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株式会社ファイン
2008.4 - 2009.2
Country：Japan
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米国イリノイ大学
2008.4 - 2008.6
Country：United States
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日本学術振興会
2005.4 - 2008.3
Country：Japan
Professional Memberships 【 display / non-display 】
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Japan Society of Nucleic Acids Chemistry
2019
Research Career 【 display / non-display 】
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Development of new DNA materials using ionic liquids
(not selected)
Project Year： 2010.1 -
Papers 【 display / non-display 】
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Stability of non-canonical nucleic acid structure as a potential modulator of cell fate. Reviewed International journal
Shuntaro Takahashi, Hisae Tateishi-Karimata, Naoki Sugimoto
Nucleic acids research 54 ( 2 ) 2026.1
Cellular morphological changes occur during cell life and diseases, such as senescence and cancer. Although the cellular conditions should be varied with the morphology changes, there have been no attempts to understand the cellular morphological changes by focusing on the intracellular molecular environment and elucidating the behaviour of nucleic acids. Nucleic acids can form hierarchical secondary and higher-order structures due to intermolecular interactions and other factors. Additionally, a number of important discoveries indicate a link between the effects of intracellular cations, hydration, and metabolic products on the stability of nucleic acid structures and diseases, such as cancer. Thus, changes in gene expression by environments can trigger morphological changes in cells. To elucidate the mechanisms of intracellular gene expression governed by nucleic acid behaviour, it is extremely important to analyse the stability of nucleic acid structures in the whole cell or local cellular spaces by manipulating the actions of small molecules, such as cations, water, and metabolic products. This review article describes the research background and latest progress in controlling senescence and cancer by modulating gene expression based on the prediction of intracellular nucleic acid behaviour, with a focus on the effects of cations, hydration, and metabolites on intracellular nucleic acid structures and their stability.
DOI： 10.1093/nar/gkaf1486
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Controlling the local conformation of RNA G-quadruplex results in reduced RNA/peptide cytotoxic accumulation associated with C9orf72 ALS/FTD Reviewed International journal
S. Matsumoto, H. Tateishi-Karimata, T. Ohyama, N. Sugimoto
Small Methods 9 ( 6 ) in press 2025.3
Authorship：Corresponding author
Repeat expansion of d(G4C2) in the noncoding region of the C9orf72 gene contributes to neurodegenerative diseases. The repeat expansion transcript r(G4C2) induces RNA/peptide accumulation, which, in turn, induces cytotoxicity and accelerates the development of neurodegenerative diseases. Such cytotoxic accumulation is triggered by peptide aggregation. Here, a technique is developed to prevent accumulation by regulating RNA interactions, assuming that RNA structure is important for peptide interactions. A screening method is used to identify compounds that suppress RNA accumulation of r(G4C2) repeats. The four compounds are identified with wide π-planes containing hydroxyl, methoxy, and cyclic ether groups that suppressed RNA accumulation. Interestingly, these compounds also suppressed RNA/peptide accumulation in neuroblastoma cells, indicating that RNA accumulation is a key regulator of RNA/peptide cytotoxic aggregate formation. In vitro and in silico physicochemical analyses reveal that these compounds bind to the loop region of the G-quadruplex via hydrogen bonds or CH-π interactions, resulting in an altered loop conformation. Importantly, these conformational changes inhibited RNA G-quadruplex associations. These results show that conformational changes are promising for controlling the interactions between G-quadruplexes and further RNA accumulation. These findings may be useful in the development of therapeutic strategies for the treatment of neurodegenerative diseases.
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Development of a Pseudocellular System to Quantify Specific Interactions Determining the G-Quadruplex Function in Cells Reviewed International journal
Hisae Tateishi-Karimata, Keiko Kawauchi, Shuntaro Takahashi, Naoki Sugimoto
Journal of the American Chemical Society 146 ( 12 ) 8005 - 8015 2024.3
Authorship：Lead author Publisher：American Chemical Society (ACS)
Intracellular chemical microenvironments, including ion concentrations and molecular crowding, play pivotal roles in cell behaviors, such as proliferation, differentiation, and cell death via regulation of gene expression. However, there is no method for quantitative analysis of intracellular environments due to their complexity. Here, we have developed a system for highlighting the environment inside of the cell (SHELL). SHELL is a pseudocellular system, wherein small molecules are removed from the cell and a crowded intracellular environment is maintained. SHELL offers two prominent advantages: (1) It allows for precise quantitative biochemical analysis of a specific factor, and (2) it enables the study of any cell, thereby facilitating the study of target molecule effects in various cellular environments. Here, we used SHELL to study G-quadruplex formation, an event that implicated cancer. We show that G-quadruplexes are more stable in SHELL compared with in vitro conditions. Although malignant transformation perturbs cellular K+ concentrations, environments in SHELL act as buffers against G-quadruplex destabilization at lower K+ concentrations. Notably, the buffering effect was most pronounced in SHELL derived from nonaggressive cancer cells. Stable G-quadruplexes form due to the binding of the G-quadruplex with K+ in different cancer cells. Furthermore, the observed pattern of G-quadruplex-induced transcriptional inhibition in SHELL is consistent with that in living cells at different cancer stages. Our results indicate that ion binding to G-quadruplexes regulates gene expression during pathogenesis.
DOI： 10.1021/jacs.3c11160
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Loss of p53 Provokes NF-κB-Dependent Disruption of Nucleolar Cap and Nucleoplasmic Redistribution of Fibrillarin During Nucleolar Stress. International journal
Takeru Torii, Mako Sumida, Atsushi Kobayashi, Toshiyuki Goto, Ryosuke Suzuki, Shin Kuwamoto, Wataru Nakajima, Wataru Sugimoto, Kohei Takeuchi, Yuma Tanaya, Masayuki Tera, Nobuyuki Tanaka, Hiroaki Hirata, Hisae Tateishi-Karimata, Takahito Nishikata, Miwako Kato Homma, Daisuke Miyoshi, Keiko Kawauchi
Biomolecules 16 ( 2 ) 2026.2
Chemotherapeutic agents targeting ribosome biogenesis induce profound reorganization of nucleolar architecture, yet how the tumor suppressor p53 governs these structural responses remains unclear. Here, we show that loss of p53 leads to NF-κB-dependent disappearance of nucleolar caps induced by doxorubicin (DOXO). Under these conditions, fibrillarin (FBL), which is normally confined to the nucleolus, relocates to the nucleoplasm and forms foci that partially associate with G-quadruplex (G4) structures, non-canonical nucleic acid secondary structures enriched at transcriptionally active genomic regions. To examine whether this redistribution is linked to transcriptional changes, we integrated publicly available transcriptomic datasets and identified genes that were upregulated in p53-deficient cells under DOXO treatment and downregulated upon FBL depletion. Given that casein kinase 2 alpha (CK2α) is a nuclear binding partner of FBL, we further analyzed CK2α-dependent gene programs. This analysis revealed that a fraction of FBL-responsive genes overlapped with CK2α-dependent signatures and were enriched for promoter-proximal G4 structures. Among candidate regulators, the G4-binding transcription factor MAZ emerged as a potential mediator linking nucleoplasmic FBL and CK2α to G4-associated transcriptional regulation. Together, our findings identify a mechanism linking loss of p53 to G4-associated transcriptional reprogramming through nucleolar architectural disruption mediated by an FBL-CK2α-MAZ axis during DOXO treatment.
DOI： 10.3390/biom16020296
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疾患進行における非二重らせん核酸構造の機能 擬似細胞系SHELLによる機構的理解
建石 寿枝, 杉本 直己
生化学 98 ( 1 ) 132 - 136 2026.2
Publisher：(公社)日本生化学会
Books and Other Publications 【 display / non-display 】
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月刊化学「化学者が思い描く未来の社会」
建石 寿枝（ Role： Joint author , ⑥非二重らせん核酸により生命の機能を制御する ―生物種を超えた統一ルール）
2021.12
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CSJカレントレビュー「進化を続ける核酸化学―ゲノム編集，非二重らせん，核酸医薬」
神谷 真子, 建石 寿枝, 永 次史, 山吉 麻子, 杉本 直己（ Role： Joint author , 第1章フロントランナーに聞く「令和の時代も進化を続ける核酸化学」）
2021.10
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相分離生物学の全貌（現代化学増刊46)
建石寿枝, 杉本直己（ Role： Joint author , 第Ⅳ部 生物学的相分離の理論）
東京化学同人 2020.11 （ ISBN:9784807913466 ）
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生体分子化学―基礎から応用まで (エキスパート応用化学テキストシリーズ)
杉本 直己, 内藤 昌信, 橋詰 峰雄, 高橋 俊太郎, 田中 直毅, 建石 寿枝, 遠藤 玉樹, 津本 浩平, 長門石 曉, 松原 輝彦, 上田 実, 朝山 章一郎
講談社 2017.1 （ ISBN:406156806X ）
Review Papers (Misc) 【 display / non-display 】
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Mechanisms of gene expression regulated by non-canonical structures of nucleic acids
Tateishi-Karimata Hisae
Journal of The Society of Japanese Women Scientists 25 37 - 42 2025.1
Publisher：The Society of Japanese Women Scientists
The structure of nucleic acids is a double helix structure (duplex) consisting of Watson-Crick base pairs. Recently, it has become clear that nucleic acids can also form non-canonical structures such as triplexes and Gquadruplexes depending on surrounding environments. Interestingly, nucleic acid sequences that can form noncanonical structures are often found on genes associated with cancer and neurodegenerative diseases. Moreover, it has been reported that the formation of non-canonical structures can induce mutations in gene expression, such as transcription and translation. The role of non-canonical structures in the onset and progression of disease has therefore attracted attention, and research to clarify the correlation between the formation of non-canonical structures and changes in disease gene expression is being conducted worldwide. These findings may help to elucidate the molecular mechanisms of disease onset and progression. This review outlines the non-canonical structures formed on disease-related genes (particularly oncogenes) and the effects of nucleic acid structures on gene expression.
DOI： 10.5939/sjws.250007
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New Data Science in Nucleic Acids Chemistry (17): Development of a pseudo-cellular system with different molecular crowding environments
TAKATSU Masako, TATEISHI-KARIMATA Hisae, SUGIMOTO Naoki
日本化学会春季年会講演予稿集(Web) 105th 2025
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New Data Science in Nucleic Acids Chemistry (15): Universal prediction of DNAzyme activity using new nearest neighbor parameters and AI
TAKAHASHI Shuntaro, TAKAHASHI Shuntaro, GHOSH Saptarshi, TATEISHI-KARIMATA Hisae, TATEISHI-KARIMATA Hisae, NISHIMURA Tomohiro, FUKUNAGA Tsukasa, HAMADA Michiaki, SUGIMOTO Naoki
日本化学会春季年会講演予稿集(Web) 105th 2025
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New Data Science in Nucleic Acids Chemistry (14): Development of methods to predict RNA secondary structures in cells
TATEISHI-KARIMATA Hisae, TATEISHI-KARIMATA Hisae, DIPANWITA Banerjee, TAKAHASHI Shuntaro, NISHIMURA Tomohiro, FUKUNAGA Tsukasa, HAMADA Michiaki, SUGIMOTO Naoki
日本化学会春季年会講演予稿集(Web) 105th 2025
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New Data Science in Nucleic Acids Chemistry (18): Comparison of the stability of DNA duplexes among normal and cancer cells
CHEN Kun, TATEISHI-KARIMATA Hisae, TAKAHASHI Shuntaro, SUGIMOTO Naoki
日本化学会春季年会講演予稿集(Web) 105th 2025
Presentations 【 display / non-display 】
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Development of a pseudo-organelle system to explore how the molecular environment shapes G-quadruplex behavior Invited
Hisae Tateishi-Karimata
2025.10
Event date： 2025.10
Country：Slovenia
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擬似細胞系を活用した細胞内核酸の構造・機能予測 Invited
建石寿枝
第25回生命化学研究会～The Final～ (ホテル モナーク鳥取) 2026.1 生命化学研究会
Event date： 2026.1
Country：Japan
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「核酸の姿と病気の関わり」 Invited
建石 寿枝
日本薬学会関西支部主催：市民公開講座「環境によって変わる核酸の姿と病気：ヒトからウイルスまでを標的とした創薬を目指して」 2022.12
Event date： 2022.12
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生物種を超えた多元応答機構の解明を目指した核酸構造の解析
建石寿枝
第95回日本生化学会大会 シンポジム「非二重らせん核酸の多元機能」 2022.11
Event date： 2022.11
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Quantitative analysis for G-quadruplex and i-motif formations in malignant cancers
Hisae Tateishi-Karimata, Naoki Sugimoto
2022.11
Event date： 2022.11
Industrial property rights 【 display / non-display 】
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核酸の立体構造を制御する方法及びその用途、並びに、細胞内分子クラウディング環境を再現するための組成物
建石 寿枝、高橋 俊太郎、川内 敬子、杉本 直己
Application no：2022-189538
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核酸の立体構造を制御する方法及びその用途、並びに、細胞内分子クラウディング環境を再現するための組成物
建石 寿枝, 川内 敬子, 高橋 俊太郎, 杉本 直己
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核酸鎖の四重螺旋構造の形成を可能にするデオキシヌクレオシド誘導体
杉本 直己, 建石 寿枝, 金原 数, 村岡 貴博
Application no：特願2015-095059
Announcement no：特開2016-210719
Patent/Registration no：特許第6802964号
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核酸塩基対の安定性を塩基対選択的に変える方法
建石 寿枝、 杉本 直己
Application no：特願2011-117381
Country of applicant：Domestic
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佐々木 義晴, 西田 尚広, 瀧上 忠一, 建石 寿枝
Academic Awards Received 【 display / non-display 】
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The Society of Japan Women Scientists for honorable mention award (2024)
2024.6
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2019年度甲南大学教員功績表彰者
2020.3 甲南大学
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日本化学会第94春季年会 優秀講演賞（学術）
2014.3 日本化学会
建石寿枝
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日本化学会第87春季年会 学生講演賞
2007.11 ａ
狩俣 寿枝 （建石の旧姓 ）
Grant-in-Aid for Scientific Research 【 display / non-display 】
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Pioneering next-generation statistical thermodynamics aimed at elucidating the functions of nucleic acid populations in the intracellular microenvironment
2025.6 - 2028.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Pioneering)
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Quantitative prediction of nucleic acid structures and functions affected by spaciotemporal environmental factors in cells
2022.4 - 2027.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(S)
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Elucidation of the mechanism for dimensional response genome using intracellular environmental evaluation systems and development of dimensional response genome bank
2021.8 - 2024.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid (B) (Tentative)
Tateishi-Karimata Hisae
In this study, we tried to clarify the mechanism of gene expression through the structure of nucleic acids across species. We have developed a pseudo-cell evaluation system and clarified the mechanism of G-quadruplex formation in cells responsive to ion concentration changes (J. Am. Chem. Soc (2024)). Furthermore, we analyzed the mechanism of gene expression by G-quadruplexes (J. Am. Chem. Soc.(2021)) and developed parameters that can predict the formation and stability of nucleic acid structures (DNA/RNA hybrid and RNA structures) that are important for gene expression (J. Am. Chem. Soc.(2023), Nucleic Acids Res.(2023)). Based on these data, we analyzed the formation of non-canonical structures and the regulation mechanism of gene expression in plants.
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2021.8 - 2024.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid (B) (Tentative)
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Elucidation of the mechanism for dimensional response genome
2021.8 - 2024.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid (B) (Tentative)
Tateishi-Karimata Hisae
The project team promoted the management and collaborative researches in our group. We held regular meetings to report research results. Moreover, advisors were invited to receive advice on the research to facilitate the smooth execution of the researches. We also organized symposiums at academic conferences in collaboration with research groups of Transformative Research Areas (A) and (B) to promote research exchange in academic societies and with other research areas. Organized two international symposiums inviting top-level researchers related to this area, and provided opportunities for discussion on the results of our works from the professors who participated in the symposiums. Messages for us from the professors were published in the newsletter and our homepage. The newsletter explaining the research results and activities of the project was published once a year and distributed to related researchers and the general public to distribute the activities of the project.
Other External funds procured 【 display / non-display 】
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極限環境により誘起されるDNA特殊構造を活用したDNAスイッチの開発
2014.4 - 2015.3
公益財団法人ひょうご科学技術協会 公益財団法人ひょうご科学技術協会 平成26年度学術研究助成
Preferred joint research theme 【 display / non-display 】
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機能性核酸および酵素の活性を溶液環境で制御することを活用したナノマテリアル（センサーなど）の開発
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細胞内で活用できる機能性核酸の開発
Social Activities 【 display / non-display 】
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なでしこscientistトーク
Role(s)： Appearance, Presenter
甲南大学 先端生命工学研究所 なでしこscientistトーク 甲南大学先端生命工学研究所 2014.6
Audience： High school students, College students, Graduate students, Teachers, Guardians, Researchesrs, General
最先端の科学技術について、女性研究者がわかりやすく解説する講演会。
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第10回なでしこscientistトーク「新型コロナウィルス感染症に挑む（COVID-19）」
Role(s)： Lecturer
甲南大学先端生命工学研究所 親和女子高等学校 2020.10
Academic Activities 【 display / non-display 】
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第95回日本生化学会大会のシンポジウム 2S09e 「非二重らせん核酸の多元機能」
Role(s)： Planning, management, etc., Panel moderator, session chair, etc.
日本生化学会 ・ 今西 未来（京都大学化学研究所)・建石 寿枝（甲南大学先端生命工学研究所(FIBER)) ・学術変革領域研究(B)「多元応答ゲノム」 （ 名古屋国際会議場 第9会場（222） ） 2022.11
Type：Competition, symposium, etc.
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B領域横断研究会（糖化学ノックイン・多元応答ゲノム）
Role(s)： Planning, management, etc., Panel moderator, session chair, etc.
学術変革領域（B）「糖化学ノックイン」「多元応答ゲノム」 （ グランフロント大阪北館 アクティブスタジオ ） 2022.10
Type：Competition, symposium, etc.
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ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～ＫＡＫＥＮＨＩ
Role(s)： Planning, management, etc., Panel moderator, session chair, etc.
甲南大学先端生命工学研究所 （ 甲南大学ポートアイランドキャンパス ） 2022.8
遺伝子を観て、新しい機能について学ぼう～mRNAワクチンやPCR検査のしくみ～
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ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～ＫＡＫＥＮＨＩ
Role(s)： Planning, management, etc., Panel moderator, session chair, etc.
甲南大学先端生命工学研究所 （ 甲南大学ポートアイランドキャンパス ） 2022.8
体験しよう PCR 検査！学ぼう遺伝子の仕組み！
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FIBER核酸化学ユニバース９
Role(s)： Panel moderator, session chair, etc.
甲南大学先端生命工学研究所 学術変革領域（B）「多元応答ゲノム」多元応答ゲノム領域推進センター （ オンライン ） 2022.2
Type：Competition, symposium, etc.
Qualification acquired 【 display / non-display 】
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High School Teacher Specialization License