写真a

TATEISHI Hisae

Position

Associate Professor

Research Field

Bio-related chemistry

Graduating School 【 display / non-display

  •  
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    2003.03

    Konan University   Faculty of Science   Graduated

Graduate School 【 display / non-display

  •  
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    2008.03

    Konan University  Graduate School, Division of Science and Technology  Doctor's Course  Completed

Campus Career 【 display / non-display

  • 2020.04
    -
    Now

    KONAN UNIVERSITYFrontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry   Associate Professor  

  • 2016.04
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    2020.03

    KONAN UNIVERSITYFrontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry   Lecturer  

  • 2010.07
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    2016.03

    KONAN UNIVERSITYFrontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry   Assistant Professor  

 

Research Career 【 display / non-display

  • Development of new DNA materials using ionic liquids

    (not selected)  

    Project Year: 2010.01  -   

Published Papers 【 display / non-display

  • Chemical biology of non-canonical structures of nucleic acids for therapeutic application

    H. Tateishi-Karimata and N. Sugimoto

    Chem. Commun.     2020.02  [Refereed]

    Joint Work

  • Effect of Molecular Crowding on the Stability of RNA G-Quadruplexes with Various Numbers of Quartets and Lengths of Loops.

    Saki Matsumoto, Hisae Tateishi-Karimata, Shuntaro Takahashi, Tatsuya Ohyama, Naoki Sugimoto

    Biochemistry   59 ( 28 ) 2640 - 2649   2020.07

    Joint Work

    G-Quadruplexes are noncanonical structures formed by guanine-rich regions of not only DNA but also RNA. RNA G-quadruplexes are widely present in the transcriptome as mRNAs and noncoding RNAs and take part in various essential functions in cells. Furthermore, stable RNA G-quadruplexes control the extent of biological functions, such as mRNA translation and antigen presentation. To understand and regulate the functions controlled by RNA G-quadruplexes in cellular environments, which are molecularly crowded, we would be required to investigate the stability of G-quadruplexes in molecular crowding. Here, we systematically investigated the thermodynamic stability of RNA G-quadruplexes with different numbers of G-quartets and lengths of loops. The molecular crowding conditions of polyethylene glycol with an average molecular weight of 200 (PEG200) were found to stabilize RNA G-quadruplexes with three and four G-quartets, while G-quadruplexes with two G-quartets did not exhibit any stabilization upon addition of PEG200. On the other hand, no difference in stabilization by PEG200 was observed among the G-quadruplexes with different loop lengths. Thermodynamic analysis of the RNA G-quadruplexes revealed more appropriate motifs for identifying G-quadruplex-forming sequences. The informatics analysis with new motifs demonstrated that the distributions of G-quadruplexes in human noncoding RNAs differed depending on the number of G-quartets. Therefore, RNA G-quadruplexes with different numbers of G-quartets may play different roles in response to environmental changes in cells.

    DOI PubMed

  • Improved nearest-neighbor parameters for the stability of RNA/DNA hybrids under a physiological condition.

    Dipanwita Banerjee, Hisae Tateishi-Karimata, Tatsuya Ohyama, Saptarshi Ghosh, Tamaki Endoh, Shuntaro Takahashi, Naoki Sugimoto

    Nucleic acids research     2020.07

    Joint Work

    The stability of Watson-Crick paired RNA/DNA hybrids is important for designing optimal oligonucleotides for ASO (Antisense Oligonucleotide) and CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9 techniques. Previous nearest-neighbour (NN) parameters for predicting hybrid stability in a 1 M NaCl solution, however, may not be applicable for predicting stability at salt concentrations closer to physiological condition (e.g. ∼100 mM Na+ or K+ in the presence or absence of Mg2+). Herein, we report measured thermodynamic parameters of 38 RNA/DNA hybrids at 100 mM NaCl and derive new NN parameters to predict duplex stability. Predicted ΔG°37 and Tm values based on the established NN parameters agreed well with the measured values with 2.9% and 1.1°C deviations, respectively. The new results can also be used to make precise predictions for duplexes formed in 100 mM KCl or 100 mM NaCl in the presence of 1 mM Mg2+, which can mimic an intracellular and extracellular salt condition, respectively. Comparisons of the predicted thermodynamic parameters with published data using ASO and CRISPR-Cas9 may allow designing shorter oligonucleotides for these techniques that will diminish the probability of non-specific binding and also improve the efficiency of target gene regulation.

    DOI PubMed

  • Nearest-neighbor parameters for predicting DNA duplex stability in diverse molecular crowding conditions.

    Saptarshi Ghosh, Shuntaro Takahashi, Tatsuya Ohyama, Tamaki Endoh, Hisae Tateishi-Karimata, Naoki Sugimoto

    Proceedings of the National Academy of Sciences of the United States of America     2020.06

    Joint Work

    The intracellular environment is crowded and heterogeneous. Although the thermodynamic stability of nucleic acid duplexes is predictable in dilute solutions, methods of predicting such stability under specific intracellular conditions are not yet available. We recently showed that the nearest-neighbor model for self-complementary DNA is valid under molecular crowding condition of 40% polyethylene glycol with an average molecular weight of 200 (PEG 200) in 100 mM NaCl. Here, we determined nearest-neighbor parameters for DNA duplex formation under the same crowding condition to predict the thermodynamics of DNA duplexes in the intracellular environment. Preferential hydration of the nucleotides was found to be the key factor for nearest-neighbor parameters in the crowding condition. The determined parameters were shown to predict the thermodynamic parameters (∆H°, ∆S°, and ∆G°37) and melting temperatures (Tm) of the DNA duplexes in the crowding condition with significant accuracy. Moreover, we proposed a general method for predicting the stability of short DNA duplexes in different cosolutes based on the relationship between duplex stability and the water activity of the cosolute solution. The method described herein would be valuable for investigating biological processes that occur under specific intracellular crowded conditions and for the application of DNA-based biotechnologies in crowded environments.

    DOI PubMed

  • RNA G-Quadruplexes Facilitate RNA Accumulation in G-Rich Repeat Expansions

    Ye Teng, Hisae Tateishi-Karimata, Naoki Sugimoto

    Biochemistry   59 ( 21 ) 1972 - 1980   2020.06

    Joint Work

    The regulatory mechanisms of the processes of RNA accumulation were examined from a chemical perspective in repeat-expansion disorders, which induce cytotoxicity and cause neurodegenerative diseases. We found that the accumulation, including production, gelation, and sedimentation, of RNA repeats transcribed from repeat expansions related to neurodegenerative diseases was greatly accelerated by G-quadruplex-forming RNA repeats, although no acceleration was induced by hairpin-forming RNA repeats. We also investigated the relationship between accumulation and physical solution properties, such as viscosity and water activity, and found that RNA accumulation was promoted through a decrease in the dielectric constant. Importantly, we found that the RNA accumulation required RNA G-quadruplexes and was promoted by changes in the dielectric property of the cell induced by an ion channel inhibitor. Our study is the first to show that the accumulation processes that induce toxicity in cells can be controlled via electrostatic interactions in the RNA G-quadruplex; thus, these can form the basis of guidelines for the chemical control of cell toxins.

    DOI PubMed

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Books etc 【 display / non-display

  • 生体分子化学―基礎から応用まで (エキスパート応用化学テキストシリーズ)

    杉本 直己, 内藤 昌信, 橋詰 峰雄, 高橋 俊太郎, 田中 直毅, 建石 寿枝, 遠藤 玉樹, 津本 浩平, 長門石 曉, 松原 輝彦, 上田 実, 朝山 章一郎 (Part: Other )

    講談社  2017.01 ISBN: 406156806X

    ASIN Amazon

  • CSJカレントレビュー「医療・診断・創薬の化学」

    建石寿枝, 杉本直己 (Part: Other ,  第4章 遺伝子診断の新手法 )

    化学同人  2017

  • 生体分子化学 : 基礎から応用まで = biomolecular chemistry

    杉本 直己, 内藤 昌信, 橋詰 峰雄, 高橋 俊太郎, 田中 直毅, 建石 寿枝, 遠藤 玉樹, 津本 浩平, 長門石 曉, 松原 輝彦, 上田 実, 朝山 章一郎, 講談社サイエンティフィク (Part: Other )

    講談社  2017 ISBN: 9784061568068

  • Facilitation of RNA enzyme activity in the molecular crowding media of cosolutes

    建石 寿枝 (Part: Single Work )

    J. Am. Chem. Soc.  2009.11

    J. Am. Chem. Soc.(学術雑誌、2009), 131, 16881-16888

  • Effects of Polyethylene Glycol on DNA Duplex Stability at Different NaCl Concentrations

    建石 寿枝 (Part: Single Work )

    Bull. Chem. Soc. Jpn.  2007.11

    Bull. Chem. Soc. Jpn.(学術雑誌、2007) 80, 10, 1987-1994

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Review Papers (Misc) 【 display / non-display

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Conference Activities & Talks 【 display / non-display

  • DNA四重らせん構造はがんの悪性化を制御しているのか

    建石寿枝, 杉本直己

    第22回生命化学研究会  (北見工業大・サロマ湖鶴雅リゾート)  2019.06  -  2019.06 

  • 神経変性疾患に関与するRNA/ペプチドによるPhase separationの機構解析

    建石寿枝, Ye Teng, 大山達也, 田中成典, 杉本直己

    第2回LLPS(液液相分離)研究会  (産業技術総合研究所)  2019.04  -  2019.04 

  • 脱ワトソン・クリックの核酸化学 (51): DNAとクラウディング分子の相互作用の分子動力学計算と電子状態計算による定量的解析

    大山達也, 建石寿枝, 田中成典, 杉本直己

    日本化学会第99回春季年会(2019)  (甲南大学(岡本キャンパス))  2019.03  -  2019.03 

  • Nucleic Acids Chemistry beyond the Watson-Crick Double Helix (49): Validation of the nearest-neighbor model for self-complementary DNA duplex under molecular crowding

    S. Ghosh, S.Takahashi, T. Endoh, H. Tateishi-Karimata, and N. Sugimoto

    日本化学会第99回春季年会(2019)  (甲南大学(岡本キャンパス))  2019.03  -  2019.03 

  • Nucleic Acids Chemistry beyond the Watson-Crick Double Helix(50):Phase separation of RNA/peptides in neurodegenerative diseases is promoted by chemical environment changes in cell

    H. Tateishi-Karimata, Y. TENG, T.Ohyama, and N. Sugimoto

    日本化学会第99回春季年会(2019)  (甲南大学(岡本キャンパス))  2019.03  -  2019.03 

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Grant-in-Aid for Scientific Research 【 display / non-display

  • Grant-in-Aid for Scientific Research(B)

    Project Year: 2020.04  -    Investigator(s): Hisae Tateishi

  • Grant-in-Aid for Scientific Research(C)

    Project Year: 2017.04  -  2020.03  Investigator(s): Hisae Tateishi

  • Grant-in-Aid for Young Scientists(B)

    Project Year: 2014.04  -  2017.03 

  • Grant-in-Aid for Young Scientists(B)

    Project Year: 2012.04  -  2014.03 

 

Qualification acquired 【 display / non-display

  • High School Teacher Specialization License