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Position |
Associate Professor |
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Research Field |
Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering, Nanotechnology/Materials / Chemistry and chemical methodology of biomolecules, Nanotechnology/Materials / Nanobioscience, Nanotechnology/Materials / Bio chemistry, Nanotechnology/Materials / Nanomaterials, Nanotechnology/Materials / Chemical biology |
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External Link |
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Graduating School 【 display / non-display 】
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Tokyo Institute of Technology Graduated
- 2001.3
Graduate School 【 display / non-display 】
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Tokyo Institute of Technology Doctor's Course Completed
- 2006.3
Campus Career 【 display / non-display 】
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KONAN UNIVERSITY Faculty of Frontiers of Innovative Research in Science and Technology Faculty of Frontiers of Innovative Research in Science and Technology Department of Nanobiochemistry Associate Professor
2016.4
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KONAN UNIVERSITY Research Institute for Nanobio-environment and Non-Ionizing Radiation (RINNIR) Director in General
2015.12
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生命化学科 講師
2009.4 - 2016.3
External Career 【 display / non-display 】
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東京工業大学大学院生命理工学研究科
2008.4 - 2009.3
Country：Japan
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日本学術振興会
2006.4 - 2008.3
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米国スクリプス研究所
2006.4 - 2008.3
Research Career 【 display / non-display 】
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nanobio-engineering, peptide engineering
Project Year： 2022.4 -
Papers 【 display / non-display 】
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Microwave-induced modulation of intracellular distribution of peptides based on mitochondrial targeting sequences Reviewed International journal
Fumihiro Kayamori, Takuto Kariya, Momo Hirata, Yonejiro Arimoto, Tamaki Endoh, Nobuhiro Nakanishi & Kenji Usui
Scientific Reports 2026.6
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Development of a Microwave-Assisted Method for Enhancing the Cellular Uptake of Arginine-Rich Peptides Reviewed International journal
Fumihiro Kayamori, Momo Hirata, Takuto Kariya, Yonejiro Arimoto, Ryuji Osawa, Mami Ishikawa, Tamaki Endoh, Nobuhiro Nakanishi, Kenji Usui
Processes 14 ( 3 ) 497 2026.1
Joint Work
Authorship：Last author, Corresponding author
Cell-penetrating peptides (CPPs) have been extensively explored as tools for the intracellular delivery of diverse molecular cargoes. Although substantial progress has been made in elucidating their uptake mechanisms and sequence-dependent functions, limitations in cellular internalization efficiency remain a major challenge, hindering their broader biomedical application. To address this issue, the present study investigated whether microwave (MW) irradiation at 2.45 GHz can enhance CPP-mediated delivery. Using confocal laser scanning microscopy and fluorescence spectroscopy, we examined the effects of MW irradiation on the cellular uptake of arginine-rich peptides. Our results suggested that MW irradiation enhanced the cellular uptake of the peptides. These findings imply that CPP-mediated delivery assisted by MW irradiation is an effective method for improving intracellular transport and may open new avenues for the development of advanced drug delivery systems.
DOI： 10.3390/pr14030497
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Synthesis of Biocompatible Gold Nanoparticles for Photothermal Therapy by Mineralization Using Peptides Reviewed International journal
Shuhei Yoshida, Koki Yoshida, Yoshiki Shitamukai, Makoto Ozaki, Takaaki Tsuruoka, Kenji Usui
RSC Advances 15 48521 - 48528 2025.12
Joint Work
Authorship：Corresponding author
DOI： https://doi.org/10.1039/d5ra08717a
Other Link： https://pubs.rsc.org/en/content/articlepdf/2025/ra/d5ra08717a
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One-Pot Synthesis and Immobilization of Gold Nanoparticles Using Peptidyl Microbeads Reviewed International journal
Shuhei Yoshida, Koki Yoshida, Taichi Isozaki, Maho Oura, Makoto Ozaki, Takaaki Tsuruoka, Kenji Usui
Molecules 30 1689 2025.4
Joint Work
Authorship：Last author, Corresponding author
Gold nanoparticles (AuNPs) have surface plasmon resonance (SPR) and catalytic activity that are not found in bulk gold and have been studied in various fields. Among these, immobilization of AuNPs on various solid-phase substrates is known to produce stable catalytic activity and specific SPRs and research on the immobilization of AuNPs has been conducted actively. However, the conventional method requires the preparation and immobilization of AuNPs in separate processes, making it difficult to prepare immobilized AuNPs in a one-pot process. In this study, we attempted to synthesize and immobilize AuNPs using peptidyl beads, which are microbeads having immobilized a peptide capable of reducing gold ions. We successfully reduced Au ions from 0.5 to 1000 µM of HAuCl4 and immobilized them on peptidyl beads in the form of AuNPs. The immobilized AuNPs have a constant particle size independent of the HAuCl4 concentration. Furthermore, the peptidyl beads with AuNPs have catalytic activity. The quantity of the AuNPs on the peptidyl beads and, subsequently, the catalytic reaction rate of the sample, could be controlled. This study would also be expected to be applied to the immobilization of metallic nanomaterials other than AuNPs by modifying the peptide sequence.
DOI： 10.3390/molecules30081689
Other Link： https://www.mdpi.com/1420-3049/30/8/1689
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Development of detection system for lead ions in mixture solutions using UV-Vis measurements with peptide immobilized microbeads Reviewed International journal
Shuhei Yoshida, Koki Yoshida, Yoshio Hamada, Takaaki Tsuruoka, Kenji Usui
Scientific Reports 15 3249 2025.1
Joint Work
Authorship：Last author, Corresponding author
Environmental pollution caused by heavy metals are problems worldwide. In particular, pollution and poisoning by lead ions (Pb2+) continue to be common and serious problems. Hence, there is a need for a widely usable method to easily detect Pb2+ from solutions containing organic materials from environmental water such as seas, ponds, etc. Here, we established a system to easily detect Pb2+ from such mixture solutions using Pb2+ binding peptide immobilized beads (peptidyl beads) and ultraviolet (UV) absorption measurements. This method could detect Pb2+ at low concentrations equivalent to inductively coupled plasmon-atomic emission spectroscopy (ICP-AES). Using the detected values to create a calibration curve, it was found that there was a positive correlation between the concentration of Pb2+ and absorbance, which also made it possible to quantify sub-µM Pb2+ in the solutions. Furthermore, Pb2+ was detected and quantified under mixed conditions of environmental water such as seas, rivers, and ponds. This method is expected to become a versatile and easy-to-use Pb2+ detection method for end-users worldwide.
DOI： 10.1038/s41598-025-86082-z
Other Link： https://www.nature.com/articles/s41598-025-86082-z
Books and Other Publications 【 display / non-display 】
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生体への影響解析のための電磁波照射装置の開発と開発研究におけるアウトリーチ活動
臼井健二、梅谷智弘（ Role： Sole author）
甲南大学総合研究所叢書 2021.3
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皮膚の安全性・有用性評価法
臼井 健二（ Role： Joint author , 第２章 第６節 ペプチド固定化樹脂を用いた皮膚感作性試験法の開発）
技術情報協会 2018.5
weblink:https://www.cmcbooks.co.jp/products/detail.php?product_id=4945
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マイクロビーズ固定化ペプチドを用いたバイオ計測デバイスの開発
臼井健二, 南野祐槻, 宮﨑洋, 横田晋一朗, 山下邦彦, 濵田芳男（ Role： Joint author）
医療・診断をささえるペプチド科学 2017.10
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バイオチップの基礎と応用―原理から最新の研究・開発動向まで―「生体分子解析・細胞解析に向けた設計ペプチドチップ」
臼井健二,堤浩,三原久和（ Role： Joint author）
シーエムシー出版 2015.10 （ ISBN:978-4-7813-1079-4 ）
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"Peptides Targeting G-Quadruplex Structures", Chemical Biology of Nucleic Acids:Fundamentals and Clinical Applications Reviewed
K. Usui, A. Okada（ Role： Joint author）
Springer 2014.6 （ ISBN:978-3-642-54452-1 ）
Review Papers (Misc) 【 display / non-display 】
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Effect of Microwave on Protein Conformations and Enzymatic Reactions Reviewed
Fumihiro Kayamori, Kenji Usui
Molecules 31 ( 11 ) 1843 - 1843 2026.5
Authorship：Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Microwave (MW) technology has attracted considerable attention as a promising approach for environmentally benign chemical processes owing to its high energy efficiency and potential for process simplification. Similarly, enzymatic reactions, which are characterized by high substrate specificity and mild reaction conditions, are indispensable technologies in both academic research and industrial applications and are widely recognized as representative green chemistry processes owing to their feasibility in aqueous media and the suppression of byproduct formation. The integration of enzymatic reactions with MW technology, referred to as MW-assisted enzymatic reactions, is therefore regarded as a promising research area. Enzymes, which are predominantly proteins and are structurally more complex than peptides, function as key catalytic entities in diverse biochemical reactions. Investigating the interactions between MWs and proteins and understanding how these interactions induce structural changes in protein molecules areessential for elucidating the effects of MW irradiation on enzymatic reactions. This review focuses on the effects of MW irradiation on protein conformations and enzymatic reactions. The insights presented herein are expected to contribute to a deeper understanding of MW-assisted enzymatic processes and the rational design of future sustainable reaction systems.
DOI： 10.3390/molecules31111843
Other Link： https://www.mdpi.com/1420-3049/31/11/1843
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ラーニング・アシスタント活用事例紹介とラーニング・アシスタント経験学生・受講生へのアンケート調査
臼井健二、梅谷智弘
甲南大学全学共通教育センター紀要 4 71 - 77 2026.3
Authorship：Lead author, Corresponding author Publishing type：Rapid communication, short report, research note, etc. (bulletin of university, research institution)
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凝集性ペプチドのモノマー化 アミロイドβペプチドを例として Reviewed
栢森 史浩、臼井 健二
化学と生物 63 ( 11 ) 480 - 482 2025.11
Authorship：Last author, Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：日本農芸化学会
凝集性ペプチドがモノマー状態からポリマー状態に変化する凝集過程を分光学的手法などによって分析するためには，そのポリマー（凝集体）をほどきペプチドをモノマー状態にすること（モノマー化）が重要である．本稿では，凝集性が高く取り扱いが難しいアミロイドβペプチドを例に，凝集性ペプチドのモノマー化について概説する． -
ラーニング・アシスタント活⽤事例紹介と ラーニング・アシスタント経験学⽣へのアンケート調査
臼井 健二
甲南大学全学共通教育センター紀要 3 191 - 196 2025.3
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ナノバイオ分野におけるマイクロ波の影響に関する研究と産業応用を目指した技術開発
栢森 史浩、有本 米次郎、中西 伸浩、臼井 健二
マイクロ波の工業応用事例集 345 - 349 2025.2
Presentations 【 display / non-display 】
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ペプチドを用いたミネラリゼーションによる光温熱療法のための異方性金ナノ構造体の作製
下向芳樹、吉田秀平、吉田光輝、鶴岡孝章、臼井健二
日本化学会第106春季年会 (日本大学理工学部 船橋キャンパス)
Event date： 2026.3
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ペプチド固相合成を題材とした基礎実験の実施と教育効果の検証
臼井健二、栢森史浩、石川真実、梅谷智弘
日本化学会第106春季年会 (日本大学理工学部 船橋キャンパス)
Event date： 2026.3
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アミロイドβペプチドの凝集における電磁波による影響解析に向けて
高橋亜衣加、栢森史浩、臼井健二
第40回関西地区ペプチドセミナー (龍谷大学 大宮キャンパス)
Event date： 2025.11
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金還元ペプチドを用いた生体応用可能な異方性金ナノ粒子を作製し光温熱療法を目指す
下向芳樹、吉田秀平、吉田光輝、臼井健二
第40回関西地区ペプチドセミナー
Event date： 2025.11
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金還元ペプチドを用いて金ナノ粒子固定化ビーズのワンポット合成法を確立する
吉田光輝、吉田秀平、鶴岡孝章、臼井健二
第40回関西地区ペプチドセミナー (龍谷大学 大宮キャンパス)
Event date： 2025.11
Industrial property rights 【 display / non-display 】
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鉛イオン結合用アフィニティ固相
臼井健二, 吉田秀平
Application no：特願2023-113327
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被験物質の皮膚感作性の検定方法
臼井健二・目片秀明・宮崎洋・山下邦彦
Application no：2016-092093
Country of applicant：Domestic
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樹脂固定ペプチド
臼井健二・目片秀明・宮崎洋・山下邦彦
Application no：2016-092094
Country of applicant：Domestic
Other Research Activities 【 display / non-display 】
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Communications Chemistry, 4, 1（2021）の論文がCommunications Chemistry誌の５周年記念記事（Communications Chemistry, 6, 33 (2023)）に”most highly cited articles”として紹介された。
2023.3
Communications Chemistry, 4, 1（2021）の論文がCommunications Chemistry誌の５周年記念記事（Communications Chemistry, 6, 33 (2023)）に”most highly cited articles”として紹介された。
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Protein & Peptide Letters誌のSpecial Issue "Organic-Inorganic Hybrid Materials and Their Applications"でゲストエディターを務めた
2016.12 - 2018.4
Protein & Peptide Letters誌のSpecial Issue "Organic-Inorganic Hybrid Materials and Their Applications"でゲストエディターを務めた
Academic Awards Received 【 display / non-display 】
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JEMEA進歩賞
2025.5 日本電磁波エネルギー応用学会
臼井健二
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生物工学研究会 鎌田泉博士論文賞
2006.3 生物工学研究会
臼井健二
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Awards of the DIMITRIS N. CHORAFAS Foundation
2005.11 Awards of the DIMITRIS N. CHORAFAS Foundation
Usui Kenji
Grant-in-Aid for Scientific Research 【 display / non-display 】
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マイクロ波による非平衡局所加熱現象を利用した位置選択的なペプチドの変換反応の開発
2024.4 - 2027.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(C)
Authorship：Coinvestigator(s)
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Evaluation method of skin sensitization using chromophore-based solid phase peptide reaction assay in vitro.
2021.4 - 2024.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(C)
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線維形成能および細胞毒性を有する短鎖ペプチド配列の予測・探索法の確立
2019.4 - 2022.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(C)
臼井 健二, 梅谷 智弘
線維形成能および細胞毒性を有する短鎖ペプチド配列の予測・探索法の確立
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Stimuli-Responsive Synthetic Ion Channels Based on Metal-Organic Polyhedra
2018.4 - 2021.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(B)
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アミロイドペプチドの凝集を規格化した細胞アッセイシステムの構築
2014.4 - 2016.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists(B)
アミロイドペプチドの凝集を規格化した細胞アッセイシステムの構築
Other External funds procured 【 display / non-display 】
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環境水中における様々な重金属イオンの簡便検出が可能なペプチド固定化ビーズの開発
2024.10 - 2025.9
公益財団法人クリタ水・環境科学振興財団 公益財団法人クリタ水・環境科学振興財団 研究助成
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医療機器におけるペプチドビーズを用いたアレルギー感作試験法
2022.1 - 2023.3
テルモ生命科学振興財団 その他財団等 テルモ生命科学振興財団・研究助成
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ペプチドビーズを用いた反応速度論による迅速・詳細なアレルギー感作試験法の開発
2021.11 - 2024.3
コーセーコスメトロジー財団 その他財団等 コスメトロジー研究助成
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[自在配列システム] 自在配列設計ペプチドによるナノポアシステムの構築
2021.10 - 2026
科学技術振興機構 CREST
Authorship：coinvestigator
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人工ペプチドと核酸を用いたミネラリゼーション制御による金ナノワイ ヤーの作製
2020.6 - 2021.5
その他財団等 村田学術振興財団 研究助成 (自然科学)
Joint and Contract research activities (Public) 【 display / non-display 】
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固定化ペプチドを利用した新規アッセイ装置の開発
Offer organization：国内民間企業 Domestic Joint Research
2015.7
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膜透過ペプチドに関する研究
Offer organization：国内大学 Domestic Joint Research
2015.4
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ペプチド線維に関する研究
Offer organization：国内民間企業 Domestic Joint Research
2014.12
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ペプチドと無機化合物に関する研究
Offer organization：龍谷大富崎研究室 Domestic Joint Research
2012.9
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ペプチドと電波に関する研究
Offer organization：株式会社ディーエスピーリサーチ Domestic Joint Research
2012.8
Preferred joint research theme 【 display / non-display 】
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細胞内での分子挙動を測定・解析できる機器の開発
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アミロイドに関する研究
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ペプチドマイクロアレイの基板開発
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固定化ペプチドの応用利用に関する研究
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ペプチドを用いた精密ナノミネラリゼーションに関する研究
Committee Memberships 【 display / non-display 】
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2026.1 特定非営利活動法人 日本電磁波エネルギー応用学会 シンポジウム実行委員
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2025.1 - 2026.3 特定非営利活動法人 日本電磁波エネルギー応用学会 シンポジウム実行委員長
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2024.5 - 2026.4 特定非営利活動法人 日本電磁波エネルギー応用学会 副理事長
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2022.5 - 2026.4 特定非営利活動法人 日本電磁波エネルギー応用学会 理事
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2021.12 - 2025.3 一般社団法人日本動物実験代替法学会 代議員
Social Activities 【 display / non-display 】
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模擬講義（西宮今津）
Role(s)： Lecturer
兵庫県立西宮今津高等学校 2024.10
Audience： High school students
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模擬講義・実験（城南）
Role(s)： Lecturer
福岡県立城南高等学校 2024.8
Audience： High school students
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模擬実験（舞子）
Role(s)： Lecturer
兵庫県立舞子高等学校 2024.8
Audience： High school students
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模擬講義（津名）
Role(s)： Lecturer
兵庫県立津名高等学校 2024.7
Audience： High school students
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模擬講義（川西緑台）
Role(s)： Appearance, Lecturer
兵庫県立川西緑台高等学校 2023.7
Audience： High school students
Qualification acquired 【 display / non-display 】
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Hazardous Material Handler (first kind)