Papers - KAWAKAMI Junji
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Efficacy of Fermented Extract Obtained from Multiple-Processed Fermentation Technology of Thermophilic Bacteria and Yeast Reviewed
Daichi Kawano, Zhengzheng Liao, Jing Nie, Akira Date, Eduardo Perez, Jose Fernandez, Corey Webb, Kristen Huber, Jeffry B. Stock, Junji Kawakami, Zihua Fu
JOURNAL OF JAPANESE COSMETIC SCIENCE SOCIETY 44 ( 3 ) 194 - 202 2020
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Enhancement of exon skipping activity by reduction in the secondary structure content of LNA-based splice-switching oligonucleotides Reviewed
T. Shimo, K. Tachibana, Y. Kawawaki, Y. Watahiki, T. Ishigaki, Y. Nakatsuji, T. Hara, J. Kawakami and S. Obika
Chem. Commun. 55 6850 - 6853 2019.4
Joint Work
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Influence of intracellular environment on allosteric ribozyme activity
Misaki Kameno, Mika Sawada, Nae Sakimoto and Junji Kawakami
178 - 179 2017.11
Joint Work
Authorship：Last author, Corresponding author
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Quantification of stabilization effect of co-solutes on RNA tertiary interaction
Natsumi Sasaki, Daisuke Miyoshi and Junji Kawakami
174 - 175 2017.11
Joint Work
Authorship：Last author, Corresponding author
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Quantitative relationship between chemical properties and bioactivities of anti-microRNA oligonucleotides targeted to tumor-associated microRNA-21 Reviewed
Koji Nagahama, Kenta Iseda, Daichi Kawano, Junji Kawakami
BIOCHIMIE 137 124 - 131 2017.6
Joint Work
Authorship：Last author, Corresponding author Publisher：ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Synthetic anti-microRNA oligonucleotides (AMOs) are promising drug candidates to inactivate disease related microRNAs because of their sequence-specific binding to their targets and the variety of chemical modifications available. Over the last decade, the qualitative relationships between the chemical properties of AMOs and bioactivity (inactivation of their target miRNAs) have been studied to enhance their bioactivity. On the other hand, in real-world drug development, drugs must be designed case-by case, taking many factors into account. Thus, in order to design AMOs that target specific miRNA, understanding the quantitative relationship between the chemical properties of AMOs and inactivation of their target miRNA is necessary. Here, we aimed to find the specific quantitative relationship of AMOs targeted to tumor-associated miR-21 through direct comparison of their inactivation efficacies with systematically varied chemical properties, including sequence-specific binding affinity, nuclease resistance, and RNase H activation. As a result, we newly found the quantitative relationships; (1) sequence specific binding affinity of AMOs against miR-21 is the main determining factor for inactivation efficacy, (2) nuclease resistance of AMOs impacts their miR-21 inactivation efficacy acting cooperatively with the binding affinity, although nuclease resistance alone does not affect the miRNA inactivation efficacy, and (3) RNase H activation is unnecessary. This study also demonstrates the utility of the obtained relationship for the design of AMO-based drugs targeted to miR-21, through cell-based analyses. Thus, the obtained quantitative relationship would make it possible to predict the miR-21 inactivation efficacy of AMOs which are newly designed. (C) 2017 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
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Influence of intracellular environment on allosteric ribozyme activity
M. Kameno, M. Sawada, N. Sakimoto, J. Kawakami
Proc. 44th Intl. Symp. Nucleic Acid Chemistry 178 - 179 2017
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Quantification of stabilization effect of co-solutes on RNA tertiary interaction
N. Sasaki, D. Miyoshi, J. Kawakami
Proc. 44th Intl. Symp. Nucleic Acid Chemistry 174 - 175 2017
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Discovery of a new function of curcumin which enhances its anticancer therapeutic potency Reviewed
Koji Nagahama, Tomoya Utsumi, Takayuki Kumano, Saeko Maekawa, Naho Oyama, Junji Kawakami
Scientific Reports 6 30962 2016.8
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Characterization of functional RNA to perform self-cleavage reaction under low pH condition
N. Sakimoto, M. Kameno, N. Sasaki, J. Kawakami
Proc. 43rd Intl. Symp. Nucleic Acid Chemistry 284 - 285 2016
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Discovery of a new function of curcumin which enhances its anticancer therapeutic potency Reviewed
K. Nagahama, T. Utsumi, T. Kumano, S. Maekawa, N. Oyama, J. Kawakami
Scientific Reports 6 30962 2016
Joint Work
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Self-Assembling Polymer Micelle/Clay Nanodisk/Doxorubicin Hybrid Injectable Gels for Safe and Efficient Focal Treatment of Cancer Reviewed
Koji Nagahama, Daichi Kawano, Naho Oyama, Ayaka Takemoto, Takayuki Kumano, Junji Kawakami
BIOMACROMOLECULES 16 ( 3 ) 880 - 889 2015.3
Joint Work
Authorship：Last author Publisher：AMER CHEMICAL SOC
The purpose of this study was to fabricate a safe and effective doxorubicin (DOX)-delivery system for focal cancer chemotherapy. A novel biodegradable injectable gel was developed through self-assembly of poly(D,L-lactide-co-glycolide)-b-poly(ethylene glycol)-b-poly(D,L-lactide-co-glycolide) (PLGA-PEG-PLGA) copolymer micelles, clay nanodisks (CNDs), and DOX. We discovered that DOX loaded in the hybrid gels acts as an anticancer drug and as a building block to organize new gel networks. Accordingly, long-term sustained release of DOX from hybrid injectable gels without initial burst release was achieved. Moreover, it was revealed that the DOX incorporated into gel networks controls its own release profile. This hybrid injectable gel is a self-controlled drug release system, which is a novel concept in controlled drug release. Importantly, a single injection of PLGA-PEG-PLGA/CND/DOX hybrid gel provides long-term sustained antitumor activity in vivo against human xenograft tumors in mice, suggesting the potential of hybrid gels as a valuable local DOX-delivery platform for cancer focal therapy.
DOI： 10.1021/bm5017805
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Comparison of Kinetic and Thermodynamic Analyses for Duplex Formation
E. Tomita, M. Sumimoto, S. Itoh, M. Takashima, and J. Kawakami
142 - 143 2015
Joint Work
Authorship：Last author, Corresponding author
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Curcumisome nanovesicles generated by self-assembly of curcumin amphiphiles toward cancer theranostics Reviewed
Koji Nagahama, Takayuki Kumano, Naho Oyama, Junji Kawakami
BIOMATERIALS SCIENCE 3 ( 12 ) 1566 - 1578 2015
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A novel ribozyme core to perform self-cleavage reaction
S. Tanaka, N. Sakimoto, M. Kameno, N. Sasaki, and J. Kawakami
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 340 - 341 2014.11
Joint Work
Authorship：Lead author
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Thermodynamic Features Provided by an ENA Introduction to Nucleic Acid Drugs
E. Tomita, M. Sumimoto, and J. Kawakami
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 188 - 189 2014.11
Joint Work
Authorship：Last author, Corresponding author
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Effects of oligonucleotide length and locked nucleic acid content on splice-switching oligonucleotide activity
T. Shimo, K. Tachibana, K. Saito, T. Yoshida, E. Tomita, R. Waki, T. Yamamoto, T. Doi, T. Inoue, J. Kawakami, and S. Obika
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 332 - 333 2014.11
Joint Work
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Design and evaluation of locked nucleic acid-based splice-switching oligonucleotides in vitro Reviewed
T. Shimo, K. Tachibana, K. Saito, T. Yoshida, E. Tomita, R. Waki, T. Yamamoto, T. Doi, T. Inoue, J. Kawakami and S. Obika
Nucleic Acids Res. 42 8174 - 8187 2014.6
Joint Work
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Thermodynamic Features Provided by an ENA Introduction to Nucleic Acid Drugs
E. Tomita, M. Sumimoto, J. Kawakami
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 188 - 189 2014
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Positional Effect of 2',4'-BNA/LNA Introduction into DNA/RNA Duplexes on Thermodynamic Parameters
S. Tahara, E. Tomita, H. Nagai, S. Obika, and J. Kawakami
Proc. 40th Intl. Symp. Nucleic Acid Chemistry 190 - 191 2013.11
Joint Work
Authorship：Lead author
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Specific recognition of cytosine by hypoxanthine in pyrrolidinyl peptide nucleic acid Reviewed International coauthorship
C. Vilaivan, W. Srinarang, N. Yotapan, W. Mansawat, C. Boonlua, J. Kawakami, Y. Yamaguchi, Y. Tanaka, and T. Vilaivan
Org. Biomol. Chem. 11 2310 - 2317 2013
Joint Work