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Position |
Professor |
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Research Field |
Nanotechnology/Materials / Chemistry and chemical methodology of biomolecules, Nanotechnology/Materials / Bio chemistry, Life Science / Pharmaceutical analytical chemistry and physicochemistry, Life Science / Molecular biology, Life Science / Structural biochemistry |
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Graduating School 【 display / non-display 】
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Graduate School of Hokkaido University Pharmaceutical Sciences Graduated
1991.4 - 1994.3
Graduate School 【 display / non-display 】
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Hokkaido University Graduate School, Division of Pharmaceutical Sciences Doctor's Course Completed
1991.4 - 1994.3
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Osaka University Graduate School, Division of Pharmaceutical Sciences Master's Course Completed
1989.4 - 1991.3
Studying abroad experiences 【 display / non-display 】
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2001.3-2002.3
Yale University Visiting Scholar
Campus Career 【 display / non-display 】
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KONAN UNIVERSITY Department of Nanobiochemistry, FIRST Professor
2009.4
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理工学部 機能分子化学科 准教授
2007.4 - 2009.3
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理工学部 機能分子化学科 助教授
2005.4 - 2007.3
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理工学部 機能分子化学科 講師
2001.4 - 2005.3
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理学部 化学科 講師
1996.4 - 2001.3
External Career 【 display / non-display 】
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Professor, Department of Nanobiochemistry, FIRST, Konan University
2009
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甲南大学 フロンティアサイエンス学部 教授
2009
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Associate Professor, Faculty of Science and Engineering, Konan University
2005 - 2009
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Visiting Fellow, Yale University
2001 - 2002
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YALE大学 客員研究員
2001 - 2002
Professional Memberships 【 display / non-display 】
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Nucleic Acids Therapeutics Society of Japan
2015.4
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The Japan Society of Nucleic Acids Chemistry
2017.11
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American Association for the Advancement of Science
1996.4
Papers 【 display / non-display 】
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Not all 2',4'-bridged modifications stabilize DNA/RNA duplexes Reviewed
Tomoka Akita, Elisa Tomita-Sudo, Shin Itoh, Nae Sakimoto, Takeshi Masuda, Akifumi Nakamura, Yoshiyuki Onishi, Makoto Koizumi Junji Kawakami
Nucleosides, Nucleotides & Nucleic Acids 2023
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製造委託の際に知っておきたい核酸医薬の特性ー品質と安全性評価面を中心に
川上 純司
Pharm Tech Japan 38 ( 12 ) 2017 - 2022 2022.9
Authorship：Lead author
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Toshiyuki Goto, Shuhei Torii, Aoi Kondo, Kazumasa Kanda, Junji Kawakami, Yosky Kataoka, Takahito Nishikata
Journal of Developmental Biology 10 ( 1 ) 10 - 10 2022.2
Publisher：MDPI AG
In many animal species, the body axis is determined by the relocalization of maternal determinants, organelles, or unique cell populations in a cytoskeleton-dependent manner. In the ascidian first cell cycle, the myoplasm, including mitochondria, endoplasmic reticulum (ER), and maternal mRNAs, move to the future posterior side concomitantly (called ooplasmic segregation or cytoplasmic and cortical reorganization). This translocation consists of first and second phases depending on the actin and microtubule, respectively. However, the transition from first to second phase, that is, translocation of myoplasmic components from microfilaments to microtubules, has been poorly investigated. In this study, we analyzed the relationship between these cytoskeletons and myoplasmic components during the first cell cycle and their role in morphogenesis by inhibitor experiments. Owing to our improved visualization techniques, there was unexpected F-actin accumulation at the vegetal pole during this transition period. When this F-actin was depolymerized, the microtubule structure was strongly affected, the myoplasmic components, including maternal mRNA, were mislocalized, and the anteroposterior axis formation was disordered. These results suggested the importance of F-actin during the first cell cycle and the existence of interactions between microfilaments and microtubules, implying the enigmatic mechanism of ooplasmic segregation. Solving this mystery leads us to an improved understanding of ascidian early development.
DOI： 10.3390/jdb10010010
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Miho Takagi-Sato, Koji Morita, Yoshiyuki Onishi, Yuuka Watahiki, Taku Ishigaki, Tomoka Akita, Erisa Tomita, Junji Kawakami, Makoto Koizumi
Nucleosides, Nucleotides & Nucleic Acids 39 ( 6 ) 838 - 852 2020.6
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Efficacy of Fermented Extract Obtained from Multiple-Processed Fermentation Technology of Thermophilic Bacteria and Yeast Reviewed
Daichi Kawano, Zhengzheng Liao, Jing Nie, Akira Date, Eduardo Perez, Jose Fernandez, Corey Webb, Kristen Huber, Jeffry B. Stock, Junji Kawakami, Zihua Fu
JOURNAL OF JAPANESE COSMETIC SCIENCE SOCIETY 44 ( 3 ) 194 - 202 2020
Books and Other Publications 【 display / non-display 】
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核酸医薬品のCMC管理戦略 −品質評価・不純物管理−
冨田恵麗沙, 秋田智香, 川上純司（ Role： Contributor , 核酸医薬品と核酸化学）
サイエンス&テクノロジー 2022.9 （ ISBN:9784864282925 ）
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実験医学 (Experimental Medicine)
秋田智香, 川上純司（ Role： Contributor , 核酸医薬の化学 総論-修飾核酸と機能評価）
実験医学 2021.11
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核酸科学ハンドブック
川上純司（ Role： Contributor , アンチセンス核酸のメカニズム）
講談社 2020.12 （ ISBN:9784065207864 ）
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ゼロからはじめるバイオ実験マスターコース③ 細胞培養トレーニング
西方敬人, 川上純司, 藤井敏司, 長濱宏治, 川内敬子（ Role： Joint author）
学研メディカル秀潤社 2015.4 （ ISBN:9784780909036 ）
Review Papers (Misc) 【 display / non-display 】
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核酸医療の基礎知識 修飾核酸 Invited
秋田 智香、冨田 恵麗沙、川上 純司
月刊 臨床神経科学 41 ( 5 ) 639 - 642 2023.5
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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Pyrrolidinyl peptide nucleic acid with α/β-peptide backbone - A conformationally constrained PNA with unusual hybridization properties
C. Vilaivan, C. Srisuwannaket, C. Ananthanawat, C. Suparpprom, J. Kawakami, Y. Yamaguchi, Y. Tanaka, T. Vilaivan
Artificial DNA: PNA & XNA 2 11 2011
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Triplet analysis that identifies unpaired regions of functional RNAs
Junji Kawakami, Yoshie Yamaguchi, Naoki Sugimoto
Journal of Nucleic Acids 2011 2011
We developed a novel method for analyzing RNA sequences, deemed triplet analysis, and applied the method in an in vitro RNA selection experiment in which HIV-1 Tat was the target. Aptamers are nucleic acids that bind a desired target (bait), and to date, many aptamers have been identified by in vitro selection from enough concentrated libraries in which many RNAs had an obvious consensus primary sequence after sufficient cycles of the selection. Therefore, the higher-order structural features of the aptamers that are indispensable for interaction with the bait must be determined by additional investigation of the aptamers. In contrast, our triplet analysis enabled us to extract important information on functional primary and secondary structure from minimally concentrated RNA libraries. As a result, by using our method, an important unpaired region that is similar to the bulge of TAR was readily predicted from a partially concentrated library in which no consensus sequence was revealed by a conventional sequence analysis. Moreover, our analysis method may be used to assess a variety of structural motifs with desired function. © 2011 Junji Kawakami et al.
DOI： 10.4061/2011/471843
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Comparative thermodynamic analysis of RNA-protein interaction on surface and in solution
Y. Tanaka, K. Ishidate, K. Kishimoto, N. Sugimoto, J. Kawakami
Proc. 37th Intl. Symp. Nucleic Acids Chemistry 276 2010
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Accurate curve fitting procedure for UV melting analysis of highly thermostable RNA hairpins
J. Kawakami, Y. Tanaka, K. Kishimoto
Nucleic Acids Symp. Ser. 53 227 2009
Presentations 【 display / non-display 】
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Efficient Method Development of Oligonucleotides by Reversed-Phase Ion-Pair Chromatography
Shinichi Fujisaki, Risa Suzuki, JunjiKawakami, Takao Inoue, Satoshi Obika, Kyoko Watanabe
TIDES USA 2023 2023.5
Event date： 2023.5
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In-process monitoring of oligonucleotide therapeutics purification using MALDI-MS
Takashi Nishikaze, JunjiKawakami, Takao Inoue, Satoshi Obika
TIDES USA 2023 2023.5
Event date： 2023.5
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ベンダーフリーな定量ソフトウェアを用いた核酸医薬品不純物の定量解析
髙嶺 竜太朗、山口 卓男、井上 貴雄、小比賀 聡、川上 純司
日本薬学会第143年会 (北海道大学) 2023.3 南 雅文
Event date： 2023.3
Country：Japan
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核酸医薬品開発の現状と課題 −国産の核酸医薬品創出に向けた取り組み−
川上 純司、井上 貴雄、小比賀 聡
日本薬学会第143年会 (北海道大学) 2023.3 南 雅文
Event date： 2023.3
Country：Japan
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核酸医薬に含まれる不純物のCDスペクトルを用いた検出
石垣 卓、大山 泰史、鈴木 仁子、神前 静香、秋田 智香、井上 貴雄、小比賀 聡、川上 純司
日本薬学会第143年会 (北海道大学) 2023.3 南 雅文
Event date： 2023.3
Country：Japan
Grant-in-Aid for Scientific Research 【 display / non-display 】
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ヘアピンループＤＮＡ／ＲＮＡを認識するモデルペプチドの速度論的機能評価
2004.4 - 2006.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists(B)
ヘアピンループＤＮＡ／ＲＮＡを認識するモデルペプチドの速度論的機能評価
領域・整理
・課題番号
4706
7305
16750150 -
ヘアピンループＤＮＡ／ＲＮＡを認識するモデルペプチドの速度論的機能評価
2004.4 - 2006.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists(B)
ヘアピンループＤＮＡ／ＲＮＡを認識するモデルペプチドの速度論的機能評価
領域・整理
・課題番号
4706
7305
16750150 -
ヘアピンループＤＮＡ／ＲＮＡを認識するモデルペプチドの速度論的機能評価
2004.4 - 2006.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists(B)
ヘアピンループＤＮＡ／ＲＮＡを認識するモデルペプチドの速度論的機能評価
領域・整理
・課題番号
4706
7305
16750150
Preferred joint research theme 【 display / non-display 】
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新規機能性核酸（アプタマー等）の取得
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リアルタイムPCR等を使用した遺伝子の定量解析
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人為的遺伝子発現調節（翻訳）
Committee Memberships 【 display / non-display 】
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2015.4 日本核酸医薬学会 評議員、幹事、事務局
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2004.4 - 2017.3 高分子学会 バイオ・高分子研究会 運営委員
Social Activities 【 display / non-display 】
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出張模擬講義
2014.12
毒学 - 薬学へのいざない／兵庫県立舞子高等学校
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模擬講義
2014.12
毒学／兵庫大学附属須磨ノ浦高等学校
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サイエンスパートナーシッププロジェクト
2014.8
自然科学入門講座「身近な生活から最先端の遺伝子研究について学ぶ」- 遺伝子鑑定／兵庫県立星陵高等学校
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etc
2014.3
遺伝子鑑定の精度・信憑性／兵庫県警察本部 刑事部
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模擬講義
2013.12
くすりの科学／須磨ノ浦女子高等学校