写真a

KAWAKAMI Junji

Position

Professor

External Link

Graduating School 【 display / non-display

  • Graduate School of Hokkaido University   Pharmaceutical Sciences   Graduated

    1991.4 - 1994.3

      More details

  • Graduate School of Osaka University   Pharmaceutical Sciences   Graduated

    1989.4 - 1991.3

      More details

  • Osaka University   Faculty of Pharmaceutical Science   Graduated

    1985.4 - 1989.3

      More details

Graduate School 【 display / non-display

  • Hokkaido University   Graduate School, Division of Pharmaceutical Sciences   Doctor's Course   Completed

    1991.4 - 1994.3

  • Osaka University   Graduate School, Division of Pharmaceutical Sciences   Master's Course   Completed

    1989.4 - 1991.3

Studying abroad experiences 【 display / non-display

  • 2001.3
    -
    2002.3

    Yale University   Visiting Scholar

Campus Career 【 display / non-display

  • KONAN UNIVERSITY   Department of Nanobiochemistry, FIRST   Professor

    2009.4

  •   理工学部 機能分子化学科   准教授

    2007.4 - 2009.3

  •   理工学部 機能分子化学科   助教授

    2005.4 - 2007.3

  •   理工学部 機能分子化学科   講師

    2001.4 - 2005.3

  •   理学部 化学科   講師

    1996.4 - 2001.3

External Career 【 display / non-display

  • Professor, Department of Nanobiochemistry, FIRST, Konan University

    2009

  • 甲南大学 フロンティアサイエンス学部 教授

    2009

  • Associate Professor, Faculty of Science and Engineering, Konan University

    2005 - 2009

  • Visiting Fellow, Yale University

    2001 - 2002

  • YALE大学 客員研究員

    2001 - 2002

display all >>

Professional Memberships 【 display / non-display

  • NUCLEIC ACIDS THERAPEUTICS SOCIETY OF JAPAN

      More details

  • Nucleic Acids Therapeutics Society of Japan

    2015.4

  • The Japan Society of Nucleic Acids Chemistry

    2017.11

  • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

      More details

  • American Association for the Advancement of Science

    1996.4

display all >>

 

Papers 【 display / non-display

  • 製造委託の際に知っておきたい核酸医薬の特性ー品質と安全性評価面を中心に

    川上 純司

    Pharm Tech Japan   38 ( 12 )   2017 - 2022   2022.9

     More details

    Authorship:Lead author  

    researchmap

  • Actin Filament in the First Cell Cycle Contributes to the Determination of the Anteroposterior Axis in Ascidian Development Reviewed

    Toshiyuki Goto, Shuhei Torii, Aoi Kondo, Kazumasa Kanda, Junji Kawakami, Yosky Kataoka, Takahito Nishikata

    Journal of Developmental Biology   10 ( 1 )   10 - 10   2022.2

     More details

    Publisher:MDPI AG  

    In many animal species, the body axis is determined by the relocalization of maternal determinants, organelles, or unique cell populations in a cytoskeleton-dependent manner. In the ascidian first cell cycle, the myoplasm, including mitochondria, endoplasmic reticulum (ER), and maternal mRNAs, move to the future posterior side concomitantly (called ooplasmic segregation or cytoplasmic and cortical reorganization). This translocation consists of first and second phases depending on the actin and microtubule, respectively. However, the transition from first to second phase, that is, translocation of myoplasmic components from microfilaments to microtubules, has been poorly investigated. In this study, we analyzed the relationship between these cytoskeletons and myoplasmic components during the first cell cycle and their role in morphogenesis by inhibitor experiments. Owing to our improved visualization techniques, there was unexpected F-actin accumulation at the vegetal pole during this transition period. When this F-actin was depolymerized, the microtubule structure was strongly affected, the myoplasmic components, including maternal mRNA, were mislocalized, and the anteroposterior axis formation was disordered. These results suggested the importance of F-actin during the first cell cycle and the existence of interactions between microfilaments and microtubules, implying the enigmatic mechanism of ooplasmic segregation. Solving this mystery leads us to an improved understanding of ascidian early development.

    DOI: 10.3390/jdb10010010

    researchmap

  • An improved synthesis of 2′-<i>O</i>,4′-<i>C</i>-ethylene nucleic acid (ENA) and thermodynamic studies of duplex formation containing the guanosine ENA unit Reviewed

    Miho Takagi-Sato, Koji Morita, Yoshiyuki Onishi, Yuuka Watahiki, Taku Ishigaki, Tomoka Akita, Erisa Tomita, Junji Kawakami, Makoto Koizumi

    Nucleosides, Nucleotides &amp; Nucleic Acids   39 ( 6 )   838 - 852   2020.6

     More details

    Publisher:Informa UK Limited  

    DOI: 10.1080/15257770.2019.1708389

    researchmap

  • Efficacy of Fermented Extract Obtained from Multiple-Processed Fermentation Technology of Thermophilic Bacteria and Yeast Reviewed

    Daichi Kawano, Zhengzheng Liao, Jing Nie, Akira Date, Eduardo Perez, Jose Fernandez, Corey Webb, Kristen Huber, Jeffry B. Stock, Junji Kawakami, Zihua Fu

    JOURNAL OF JAPANESE COSMETIC SCIENCE SOCIETY   44 ( 3 )   194 - 202   2020

     More details

  • Different reactivity of Sp and Rp isomers of phosphorothioate-modified oligonucleotides in a duplex structure Reviewed

    Md Ariful Islam, Aki Fujisaka, Junji Kawakami, Takao Yamaguchi, Satoshi Obika

    Bioorg. Med. Chem. Lett.   30 ( 14 )   127166 - 127166   2020

     More details

    Joint Work

    Publisher:Elsevier BV  

    DOI: 10.1016/j.bmcl.2020.127166

    researchmap

display all >>

Books and Other Publications 【 display / non-display

  • 核酸医薬品のCMC管理戦略 −品質評価・不純物管理−

    冨田恵麗沙, 秋田智香, 川上純司( Role: Contributor ,  核酸医薬品と核酸化学)

    サイエンス&テクノロジー  2022.9  ( ISBN:9784864282925

     More details

  • 実験医学 (Experimental Medicine)

    秋田智香, 川上純司( Role: Contributor ,  核酸医薬の化学 総論-修飾核酸と機能評価)

    実験医学  2021.11 

     More details

  • 核酸科学ハンドブック

    川上純司( Role: Contributor ,  アンチセンス核酸のメカニズム)

    講談社  2020.12  ( ISBN:9784065207864

     More details

  • 医薬品開発における中分子領域(核酸医薬・ペプチド医薬)の開発戦略

    川上 純司(アンチセンス核酸医薬の作用機序)

    情報機構  2019.10 

     More details

  • ゼロからはじめるバイオ実験マスターコース③ 細胞培養トレーニング

    西方敬人, 川上純司, 藤井敏司, 長濱宏治, 川内敬子( Role: Joint author)

    学研メディカル秀潤社  2015.4  ( ISBN:9784780909036

display all >>

Review Papers (Misc) 【 display / non-display

  • Pyrrolidinyl peptide nucleic acid with α/β-peptide backbone - A conformationally constrained PNA with unusual hybridization properties

    C. Vilaivan, C. Srisuwannaket, C. Ananthanawat, C. Suparpprom, J. Kawakami, Y. Yamaguchi, Y. Tanaka, T. Vilaivan

    Artificial DNA: PNA & XNA   2   11   2011

     More details

  • Triplet analysis that identifies unpaired regions of functional RNAs

    Junji Kawakami, Yoshie Yamaguchi, Naoki Sugimoto

    Journal of Nucleic Acids   2011   2011

     More details

    We developed a novel method for analyzing RNA sequences, deemed triplet analysis, and applied the method in an in vitro RNA selection experiment in which HIV-1 Tat was the target. Aptamers are nucleic acids that bind a desired target (bait), and to date, many aptamers have been identified by in vitro selection from enough concentrated libraries in which many RNAs had an obvious consensus primary sequence after sufficient cycles of the selection. Therefore, the higher-order structural features of the aptamers that are indispensable for interaction with the bait must be determined by additional investigation of the aptamers. In contrast, our triplet analysis enabled us to extract important information on functional primary and secondary structure from minimally concentrated RNA libraries. As a result, by using our method, an important unpaired region that is similar to the bulge of TAR was readily predicted from a partially concentrated library in which no consensus sequence was revealed by a conventional sequence analysis. Moreover, our analysis method may be used to assess a variety of structural motifs with desired function. © 2011 Junji Kawakami et al.

    DOI: 10.4061/2011/471843

    researchmap

  • Comparative thermodynamic analysis of RNA-protein interaction on surface and in solution

    Y. Tanaka, K. Ishidate, K. Kishimoto, N. Sugimoto, J. Kawakami

    Proc. 37th Intl. Symp. Nucleic Acids Chemistry   276   2010

     More details

  • Accurate curve fitting procedure for UV melting analysis of highly thermostable RNA hairpins

    J. Kawakami, Y. Tanaka, K. Kishimoto

    Nucleic Acids Symp. Ser.   53   227   2009

     More details

  • Recognition of a flipped base in a hairpinloop DNA by a small peptide

    Junji Kawakami, Shinji Okabe, Yoshiatsu Tanabe, Naoki Sugimoto

    NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS   27 ( 3 )   292 - 308   2008.3

     More details

    Publisher:TAYLOR & FRANCIS INC  

    Two tiny hairpin DNAs, CORE (dAGGCTTCGGCCT) and AP2 (dAGGCTXCGGCCT,X:abasic nucleotide), fold into almost the same tetraloop hairpin structure with one exception, that is, the sixth thymine (T6) of CORE is exposed to the solvent water (Kawakami, J et al., Chem. Lett. 2001, 258-259). In the present study, we selected small peptides that bind to CORE or AP2 from a combinatorial pentapeptide library with 2.5 x 106 variants. On the basis of the structural information, the selected peptide sequences should indicate the essential qualifications for recognition of the hairpin loop DNA with and without a flipped base. In the selected DNA binding peptides, aromatic amino acids such as histidine for CORE and glutamine/aspartic acid for AP2 were found to be abundant amino acids. This amino acid preference suggests that CORE-binding peptides use)pi-pi stacking to recognize the target while hydrogen bonding is dominant for AP2-binding peptides. To investigate the binding properties of the selected peptide to the target, surface plasmon resonance was used. The binding constant of the interaction between CORE and a CORE-binding peptide (HWHHE) was about 1.1 x 10(6) M-1 at 25 degrees C and the resulting binding free energy change at 25 degrees C (Delta G(25)degrees) was - 8.2 kcal mol(-1). The binding of the peptide to AP2 was also analyzed and the resulting binding constant and Delta G(25)degrees were about 4.2 x 10(4) M-1 and -63 kcal mol(-1), respectively. The difference in the binding free energy changes (Delta Delta G(25)degrees) of 1.9 kcal mol(-1) was comparable to the values reported in other systems and was considered a consequence of the loss of pi-pi stacking. Moreover, the stabilization effect by stacking affected the dissociation step as well as the association step. Our results suggest that the existence Of an aromatic ring (T6 base) produces new dominant interactions between peptides and nucleic acids, although hydrogen bonding is the preferable mode of interaction in the absence of the flipping base. These findings regarding CORE and AP2 recognition are expected to give useful information in the design of novel artificial DNA binding peptides.

    DOI: 10.1080/15257770701845261

    researchmap

display all >>

Presentations 【 display / non-display

  • Evaluation of UV-protective effect of Activating polyphenol grape seed extract

    Daichi KAWANO, Junji KAWAKAMI, Zihua FU

    The 45th Annual Meeting of the Molecular Biology Society of Japan  (Makuhari Messe)  2022.12  Tatsuo Fukagawa

     More details

    Event date: 2022.11 - 2022.12

  • Effect of RNA length on thermodynamic changes during duplex formation with complementary antisense therapeutics

    Elisa Tomita-Sudo, omoka Akita, Ayumu Kashiwagi, Renshin Sano, Riki Hatakenaka, Takao Inoue, Satoshi Obika, junji Kawakami

    The 45th Annual Meeting of the Molecular Biology Society of Japan  (Makuhari Messe)  2022.11  Tatsuo Fukagawa

     More details

    Event date: 2022.11 - 2022.12

  • Thermodynamic stability of an aptamer with G-quadruplex structure under K+/Na+ coexisting conditions.

    Taku Ishigaki, Masako Hirose, Maki Kato, Junji Kawakami

    The 45th Annual Meeting of the Molecular Biology Society of Japan  (Makuhari Messe)  2022.11  Tatsuo Fukagawa

     More details

    Event date: 2022.11 - 2022.12

  • Sequence optimization of a ligase ribozyme for circRNA preparation

    Akane Kiyose, Junji Kawakami

    The 45th Annual Meeting of the Molecular Biology Society of Japan  (Makuhari Messe)  2022.11  Tatsuo Fukagawa

     More details

    Event date: 2022.11 - 2022.12

  • 遺伝子を注射するコロナワクチン Invited

    川上 純司

    ホテルグランヴィア大阪 講演会  2022.11 

     More details

    Event date: 2022.11

display all >>

Grant-in-Aid for Scientific Research 【 display / non-display

  • ヘアピンループDNA/RNAを認識するモデルペプチドの速度論的機能評価

    2004.4 - 2006.3

    JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists(B)

      More details

    ヘアピンループDNA/RNAを認識するモデルペプチドの速度論的機能評価
    領域・整理
    ・課題番号
    4706
    7305
    16750150

  • ヘアピンループDNA/RNAを認識するモデルペプチドの速度論的機能評価

    2004.4 - 2006.3

    JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists(B)

      More details

    ヘアピンループDNA/RNAを認識するモデルペプチドの速度論的機能評価
    領域・整理
    ・課題番号
    4706
    7305
    16750150

  • ヘアピンループDNA/RNAを認識するモデルペプチドの速度論的機能評価

    2004.4 - 2006.3

    JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists(B)

      More details

    ヘアピンループDNA/RNAを認識するモデルペプチドの速度論的機能評価
    領域・整理
    ・課題番号
    4706
    7305
    16750150

Preferred joint research theme 【 display / non-display

  • 新規機能性核酸(アプタマー等)の取得

  • リアルタイムPCR等を使用した遺伝子の定量解析

  • 人為的遺伝子発現調節(翻訳)

 

Committee Memberships 【 display / non-display

  • 2015.4   日本核酸医薬学会  評議員、幹事、事務局

  • 2016.9   日本核酸化学会  評議員

      More details

  • 2004.4 - 2017.3   高分子学会  バイオ・高分子研究会 運営委員

  • 2015.12   日本核酸医薬学会  事務局長

      More details

  • 2015.4   日本核酸医薬学会  評議員

      More details

display all >>

Social Activities 【 display / non-display

  • 出張模擬講義

    2014.12

     More details

    毒学 - 薬学へのいざない/兵庫県立舞子高等学校

  • 模擬講義

    2014.12

     More details

    毒学/兵庫大学附属須磨ノ浦高等学校

  • サイエンスパートナーシッププロジェクト

    2014.8

     More details

    自然科学入門講座「身近な生活から最先端の遺伝子研究について学ぶ」- 遺伝子鑑定/兵庫県立星陵高等学校

  • etc

    2014.3

     More details

    遺伝子鑑定の精度・信憑性/兵庫県警察本部 刑事部

  • 模擬講義

    2013.12

     More details

    くすりの科学/須磨ノ浦女子高等学校

display all >>