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Position |
特任研究准教授 |
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Research Field |
Life Science / Genetics, Life Science / Neuroscience-general, Life Science / Molecular biology |
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Profile |
動物は環境変化に応じて、体内環境を適正に変容させることで生存や繁栄することができます。これまでに温度に対する動物の応答に関して、分子遺伝学的な解析を進めてきました。具体的には、動物がどのように温度を感知するのか、そしてどのように過去の温度情報を記憶し適応するのかを解き明かすために、線虫C. elegansの温度に対する応答である低温耐性と温度馴化を実験モデルとして解析してきました。この新しい実験系から次々と新しいことが見つかってきています。線虫は小さなモデル動物ですが、ホットな研究に繋がればと思います。 |
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Graduating School 【 display / non-display 】
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Nagoya University Faculty of Science Department of Biological Science Graduated
1997 - 2001
Graduate School 【 display / non-display 】
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Nagoya University Graduate School, Division of Medicine Doctor's Course Completed
2008.4 - 2011
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Nagoya University Graduate School, Division of Natural Science Master's Course Completed
2001.3 - 2003.3
External Career 【 display / non-display 】
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日本学術振興会 特別研究員RPD / 甲南大学特別研究員（兼任）
2019 - 2022.6
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日本学術振興会 特別研究員PD / 甲南大学特別研究員（兼任）
2016 - 2019
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日本学術振興会 特別研究員RPD / 甲南大学特別研究員（兼任）
2014 - 2016
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甲南大学大学院自然科学研究科 科研研究員
2011 - 2014
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日本学術振興会 特別研究員DC2
2009 - 2011
Professional Memberships 【 display / non-display 】
Papers 【 display / non-display 】
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Haruka Motomura, Makoto Ioroi, Kazutoshi Murakami, Atsushi Kuhara, Akane Ohta
PNAS 119 ( 32 ) 1 - 9 2022.8
Authorship：Lead author, Last author, Corresponding author Publisher：Proceedings of the National Academy of Sciences
Animals maintain the ability to survive and reproduce by acclimating to environmental temperatures. We showed here that Caenorhabditis elegans exhibited temperature acclimation plasticity, which was regulated by a head-tail-head neural circuitry coupled with gut fat storage. After experiencing cold, C. elegans individuals memorized the experience and were prepared against subsequent cold stimuli. The cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) regulated temperature acclimation in the ASJ thermosensory neurons and RMG head interneurons, where it modulated ASJ thermosensitivity in response to past cultivation temperature. The PVQ tail interneurons mediated the communication between ASJ and RMG via glutamatergic signaling. Temperature acclimation occurred via gut fat storage regulation by the triglyceride lipase ATGL-1, which was activated by a neuropeptide, FLP-7, downstream of CREB. Thus, a head-tail-head neural circuit coordinated with gut fat influenced experience-dependent temperature acclimation.
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OSM-9 and OCR-2 TRPV channels are accessorial warm receptors in Caenorhabditis elegans temperature acclimatisation Reviewed International journal
Ohnishi K, Saito S, Miura Y, Ohta A, Tominaga M, Sokabe T, Kuhara A
Scientific Reports 10 ( 18566 ) 1 - 14 2020.10
Caenorhabditis elegans (C. elegans) exhibits cold tolerance and temperature acclimatisation regulated by a small number of head sensory neurons, such as the ADL temperature-sensing neurons that express three transient receptor potential vanilloid (TRPV) channel subunits, OSM-9, OCR-2, and OCR-1. Here, we show that an OSM-9/OCR-2 regulates temperature acclimatisation and acts as an accessorial warmth-sensing receptor in ADL neurons. Caenorhabditis elegans TRPV channel mutants showed abnormal temperature acclimatisation. Ectopic expression of OSM-9 and OCR-2 in non-warming-responsive gustatory neurons in C. elegans and Xenopus oocytes revealed that OSM-9 and OCR-2 cooperatively responded to warming; however, neither TRPV subunit alone was responsive to warming. A warming-induced OSM-9/OCR-2-mediated current was detectable in Xenopus oocytes, yet ADL in osm-9 ocr-2 double mutant responds to warming; therefore, an OSM-9/OCR-2 TRPV channel and as yet unidentified temperature receptor might coordinate transmission of temperature signalling in ADL temperature-sensing neurons. This study demonstrates direct sensation of warming by TRPV channels in C. elegans.
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The mechanoreceptor DEG-1 regulates cold tolerance in Caenorhabditis elegans. Reviewed International journal
Natsune Takagaki, Akane Ohta, Kohei Ohnishi, Akira Kawanabe, Yohei Minakuchi, Atsushi Toyoda, Yuichiro Fujiwara, Atsushi Kuhara
EMBO reports e48671 ( Article ) e48671 - 12 2020.2
Authorship：Corresponding author
Caenorhabditis elegans mechanoreceptors located in ASG sensory neurons have been found to sense ambient temperature, which is a key trait for animal survival. Here, we show that experimental loss of xanthine dehydrogenase (XDH-1) function in AIN and AVJ interneurons results in reduced cold tolerance and atypical neuronal response to changes in temperature. These interneurons connect with upstream neurons such as the mechanoreceptor-expressing ASG. Ca2+ imaging revealed that ASG neurons respond to warm temperature via the mechanoreceptor DEG-1, a degenerin/epithelial Na+ channel (DEG/ENaC), which in turn affects downstream AIN and AVJ circuits. Ectopic expression of DEG-1 in the ASE gustatory neuron results in the acquisition of warm sensitivity, while electrophysiological analysis revealed that DEG-1 and human MDEG1 were involved in warm sensation. Taken together, these results suggest that cold tolerance is regulated by mechanoreceptor-mediated circuit calculation.
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Cold acclimation via the KQT-2 potassium channel is modulated by oxygen in Caenorhabditis elegans. Reviewed International coauthorship International journal
Misaki Okahata, Aguan D Wei, Akane Ohta, Atsushi Kuhara
Science advances 5 ( 2 ) eaav3631 - 12 2019.2
Authorship：Corresponding author
Adaptive responses to external temperatures are essential for survival in changing environments. We show here that environmental oxygen concentration affects cold acclimation in Caenorhabditis elegans and that this response is regulated by a KCNQ-type potassium channel, KQT-2. Depending on culture conditions, kqt-2 mutants showed supranormal cold acclimation, caused by abnormal thermosensation in ADL chemosensory neurons. ADL neurons are responsive to temperature via transient receptor potential channels-OSM-9, OCR-2, and OCR-1-with OCR-1 negatively regulating ADL function. Similarly, KQT-2 and KQT-3 regulate ADL activity, with KQT-2 positively regulating ADL function. Abnormal cold acclimation and acute temperature responses of ADL neurons in kqt-2 mutants were suppressed by an oxygen-receptor mutation in URX coelomic sensory neurons, which are electrically connected to ADL via RMG interneurons. Likewise, low oxygen suppressed supranormal kqt-2 cold acclimation. These data thus demonstrate a simple neuronal circuit integrating two different sensory modalities, temperature and oxygen, that determines cold acclimation.
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Endoribonuclease ENDU-2 regulates multiple traits including cold tolerance via cell autonomous and nonautonomous controls in C. elegans Reviewed International coauthorship
Ujisawa T, Ohta A, Ii T, Minakuchi Y, Toyoda A, Ii M, Kuhara A
PNAS 115 ( 35 ) 8823 - 8828 2018
Books and Other Publications 【 display / non-display 】
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匂いに慣れたらどうなるの？センチュウの匂い順応テスト：嗅覚順応行動の測定
太田茜, 園田悟, 久原篤（ Role： Contributor , 研究者が教える動物実験 第1巻 感覚,p42-45）
共立出版 比較生理生化学会編 2015
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遺伝子の突然変異のDNAを見てみよう PCR法と制限酵素による突然変異部位の可視化
太田茜, 園田悟, 久原篤（ Role： Contributor , 研究者が教える動物実験 第3巻 行動,p40-43）
共立出版 比較生理生化学会編 2015
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緑色にキラリと光る細胞を見てみよう 緑色蛍光タンパク質GFPによる神経細胞の観察
久原篤, 太田茜（ Role： Contributor , 研究者が教える動物実験 第2巻 神経・筋,p20-23）
共立出版 比較生理生化学会編 2015
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好きな温度に向かう行動を調べよう センチュウの温度走性テスト：温度応答行動の測定
久原篤, 太田茜（ Role： Contributor , 研究者が教える動物実験 第3巻 行動,p36-39）
共立出版 比較生理生化学会編 2015
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好きなにおいに向かっていく行動を見てみよう センチュウの走化性テスト：嗅覚応答行動の測定
太田茜, 園田悟, 久原篤（ Role： Contributor , 研究者が教える動物実験 第3巻 行動,p32-35）
共立出版 比較生理生化学会編 2015
Review Papers (Misc) 【 display / non-display 】
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Imaging and manipulation of temperature response of nerve cells in brain-gut interaction underlying cold tolerance Invited
5 ( 6 ) 55 - 61 2021.6
Authorship：Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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Isolation of novel temperature signaling molecule via high throughput experimental system Invited Reviewed
Haruka Motomura, Natsune Takagaki, Atsushi Kuhara, Akane Ohta
月刊 細胞、特集 細胞の温度を測る 52 ( 14 ) 37(823) - 40(826) 2020.12
Authorship：Last author, Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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Analysis of temperature sensation by single cell analysis using optgenetics Invited Reviewed
Okahata M, Motomura H, Ohta A, Kuhara A
月刊「細胞」特集 単一細胞レベル解析の新技術 52 ( 13 ) 28(678) - 31(681) 2020.10
Authorship：Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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線虫 Caenorhabditis elegansの低温馴化における温度感覚は酸素濃度の影響を受ける Reviewed
岡畑 美咲, 太田 茜, 久原 篤
比較生理生化学 37 ( 2 ) 103 - 110 2020.8
Authorship：Corresponding author
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Glia-neuron interaction in the nematode amphid sensory system and cold tolerance Reviewed
月刊「細胞」 50 ( 9 ) 480 - 483 2018.8
Academic Awards Received 【 display / non-display 】
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動物学会 奨励賞
2023.9 日本動物学会
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神経科学会 奨励賞
2023.8 日本神経科学会
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保井コノ賞
2023.2
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守田科学研究奨励賞
2017.1 大学女性協会
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日本動物学会女性研究者奨励OM賞
2016.1 日本動物学会
Grant-in-Aid for Scientific Research 【 display / non-display 】
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https://researchmap.jp/aohta
JSPS Grants-in-Aid for Scientific Research リサーチマップ参照
Other External funds procured 【 display / non-display 】
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https://researchmap.jp/aohta
1000
リサーチマップ参照 リサーチマップ参照 リサーチマップ参照
Media Coverage 【 display / non-display 】
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http://kuharan.com/publications/press.html#n2017 TV or radio program