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Position |
Associate Professor |
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Research Field |
Life Science / Molecular biology, Life Science / Tumor biology |
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External Link |
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Graduating School 【 display / non-display 】
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Himeji Institute of Technology Faculty of Science Graduated
1991.4 - 1995.3
Graduate School 【 display / non-display 】
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姫路工業大学大学院 理学研究科 生命科学専攻 Doctor's Course Completed
1998.4 - 2002.3
Campus Career 【 display / non-display 】
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KONAN UNIVERSITY Faculty of Frontiers of Innovative Research in Science and Technology Faculty of Frontiers of Innovative Research in Science and Technology Department of Nanobiochemistry Associate Professor
2018.4
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KONAN UNIVERSITY Faculty of Frontiers of Innovative Research in Science and Technology Faculty of Frontiers of Innovative Research in Science and Technology Department of Nanobiochemistry Lecturer
2014.4 - 2018.3
External Career 【 display / non-display 】
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神戸大学大学院 工学研究科
2021.12
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日本医科大学 先端医学研究所
2014.4 - 2021.3
Country：Japan
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National University of Singapore Mechanobiology Institute
2010.5 - 2014.3
Country：Singapore
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日本医科大学 老人病研究所
2008.10 - 2011.12
Country：Japan
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日本医科大学 老人病研究所
2007.4 - 2008.9
Country：Japan
Papers 【 display / non-display 】
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Phase Separation of RX Repeat Peptides with Nucleic Acids
Sumit Shil, Mitsuki Tsuruta, Ryosuke Suzuki, Yoshiki Hashimoto, Takeru Torii, Shinya Taniguchi, Tomohiro Umetani, Keiko Kawauchi, Daisuke Miyoshi
Chemistry – An Asian Journal 2025.10
Publisher：Wiley
Abstract
Biomolecular liquid‐liquid phase separation (LLPS) plays a crucial role in organizing membraneless cellular compartments, which regulate a wide variety of cellular processes. A key molecular mechanism underlying LLPS of nucleic acids involves G‐quadruplex (G4) structures of DNA and RNA interacting with intrinsically disordered proteins, particularly arginine and glycine (RGG/RG) rich proteins. The role of arginine residues in LLPS has been studied extensively, whereas few studies have focused on the role of the another frequently occurring residues, glycine. Here, we systematically investigated the contribution of G residues by substituting them with alanine (A), proline (P), valine (V), and tyrosine (Y) residues, generating a series of RX repeat peptides. Turbidity and microscopy assays with DNA oligonucleotides forming G4, duplex, as well as random coil, showed that RP and RA‐peptides enhanced LLPS with G4 DNA, by comparing RG‐peptide. In contrast, RY promoted liquid‐solid phase separation (LSPS) with the G4 DNA, although it underwent LLPS with the random coil and duplex DNAs. In addition, RV‐peptide formed aggregates even in the absence of any DNA. These results demonstrate that side‐chain size, hydrophobicity, and aromaticity are critical factors for the LLPS and LSPS capability and selectivity with DNA forming various secondary structures. This study provides mechanistic insights into protein‐nucleic acid LLPS and LSPS and guides the rational design peptides to undergo LLPS but not LSPS with nucleic acids. -
Dynamic Remodeling of Mechano-Sensing Complexes in Suspended Fibroblast Cell-Sheets Under External Mechanical Stimulus Reviewed International journal
Madoka Suzuki, Keiko Kawauchi, Hiroaki Machiyama, Hiroaki Hirata, Shin'ichi Ishiwata, Hideaki Fujita
Biotechnology and Bioengineering 122 ( 7 ) 1929 - 1940 2025.7
DOI： 10.1002/bit.28996
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Three- and four-stranded nucleic acid structures and their ligands. International journal
Yoshiki Hashimoto, Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, Daisuke Miyoshi
RSC chemical biology 6 ( 4 ) 466 - 491 2025.4
Nucleic acids have the potential to form not only duplexes, but also various non-canonical secondary structures in living cells. Non-canonical structures play regulatory functions mainly in the central dogma. Therefore, nucleic acid targeting molecules are potential novel therapeutic drugs that can target 'undruggable' proteins in various diseases. One of the concerns of small molecules targeting nucleic acids is selectivity, because nucleic acids have only four different building blocks. Three- and four-stranded non-canonical structures, triplexes and quadruplexes, respectively, are promising targets of small molecules because their three-dimensional structures are significantly different from the canonical duplexes, which are the most abundant in cells. Here, we describe some basic properties of the triplexes and quadruplexes and small molecules targeting the triplexes and tetraplexes.
DOI： 10.1039/d4cb00287c
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The role of cytosine methylation in regulating the topology and liquid-liquid phase separation of DNA G-quadruplexes. International journal
Mitsuki Tsuruta, Sumit Shil, Shinya Taniguchi, Keiko Kawauchi, Daisuke Miyoshi
Chemical science 16 ( 10 ) 4213 - 4225 2025.3
Aberrant expansion of GGGGCC DNA repeats that form G-quadruplexes (G4) is the main cause of amyotrophic lateral sclerosis (ALS). Expanded GGGGCC repeats induce liquid-liquid phase separation (LLPS) through their interaction with cellular proteins. Furthermore, GGGGCC expansion induces cytosine methylation (mC). Previous studies have shown that even slight chemical modifications of RNAs and proteins can drastically affect their LLPS ability, yet the relationship between LLPS and epigenetic DNA modifications like mC remains unexplored. As a model system, we investigated the effects of mC on LLPS induced by GGGGCC repeat DNAs and show for the first time that mC suppresses LLPS by altering the topology of G4 from being parallel to antiparallel.
DOI： 10.1039/d4sc06959e
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Roles of Loop Region in Folding Kinetics and Transcription Inhibition of DNA G-Quadruplexes Reviewed
64 609 - 619 2025.2
Books and Other Publications 【 display / non-display 】
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分散・凝集技術ハンドブック
鶴田充生・川内敬子・三好大輔（ Role： Joint author , 第６章 バイオ分野 第３節 細胞内液-液相分離：核酸、タンパク質の凝集と機能）
凝集 株式会社エヌ・ティー・エス 2025.4
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がん研究読本 6
川内敬子（RASによるがん悪性化に、p53を介したアクチン細胞骨格の変化がブレーキをかける道筋を解明！）
がん研究分野の特性等を踏まえた支援活動総括支援活動班 2016
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ゼロからはじめるバイオ実験マスター3細胞培養トレーニング
西方敬人, 川上純司, 藤井敏司, 長濱宏治, 川内敬子（ Role： Joint author）
学研メディカル集潤社 2015.3
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細胞培養トレーニング
西方 敬人, 川上 純司, 藤井 敏司, 長濱 宏治, 川内 敬子
学研メディカル秀潤社, 学研マーケティング (発売) 2015 （ ISBN:9784780909036 ）
Review Papers (Misc) 【 display / non-display 】
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Three- and four-stranded nucleic acid structures and their ligands Reviewed
6 466 - 491 2025.2
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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がん免疫療法におけるCAR細胞療法の多様性とその展望 Reviewed
出川詩織、川内敬子、西方敬人
日本女性科学者の会学術誌 25 43 - 48 2025.1
Authorship：Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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日本酒麹菌産生物質デフェリフェリクリシンががん細胞に及ぼす効果について Invited
月生雅也、取井猛流、木下菜月、戸所健彦、石田博樹、西方敬人、川内敬子
食と医療 29 14 - 20 2024.4
Authorship：Last author, Corresponding author Publishing type：Article, review, commentary, editorial, etc. (other)
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細胞内環境で安定化する核酸構造を標的にした低分子薬の開発 Reviewed
橋本 佳樹, 川内 敬子, 三好 大輔
MEDCHEM NEWS 34 36 - 42 2024.4
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
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RNA高次構造を標的とした光線力学的療法の展望 Invited
BIO Clinica 38 ( 13 ) 76 - 78 2023.11
Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
Presentations 【 display / non-display 】
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Crosstalk between actin remodeling and p53 signaling in the DNA damage response
2024.11
Event date： 2024.11
Country：Japan
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Crosstalk between actin remodeling and p53 signaling in the DNA damage response
2024.9
Event date： 2024.9
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グアニン四重らせん構造により制御される長鎖非コードRNA Invited
川内敬子、三好大輔
第46回日本分子生物学会 (兵庫) 2023.12
Event date： 2023.12
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グアニン四重らせん構造により制御される長鎖非コードRNA Invited
川内敬子、三好大輔
第46回日本分子生物学会シンポジウム (兵庫) 2023.12 日本分子生物学会
Event date： 2023.12
Country：Japan
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Emerging roles of actin in p53-dependent DNA damage responses Invited
Keiko Kawauchi
The 10th International MDM2 workshop 2023.10
Event date： 2023.10
Country：Japan
Industrial property rights 【 display / non-display 】
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核酸の立体構造を制御する方法及びその用途、並びに、細胞内分子クラウディング環境を再現するための組成物
建石 寿枝、川内 敬子、高橋 俊太郎、杉本 直己
Application no：特願2022-189538
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光増感剤輸送キャリア
大谷 亨、川内 敬子、三好 大輔
Application no：特願2022-128296
Academic Awards Received 【 display / non-display 】
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村尾育英会 学術賞
2019.3 一般財団法人 村尾育英会
川内敬子
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神奈川難病財団研究奨励賞
2008.12 神奈川難病財団
川内敬子
Grant-in-Aid for Scientific Research 【 display / non-display 】
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Study for Inverse Correlation in Neurodegenerative Disease and Cancer Focusing on Liquid-Liquid Phase Separation of Nucleic Acids
2024.6 - 2026.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
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The regulation of gene expression by targeting G4 quadriplex in brain tumors
2023.4 - 2026.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(B)
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生体分子の液液相分離制御工学構築による新規創薬モダリティの提唱
2022.6 - 2025.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Pioneering)
三好 大輔, 川内 敬子
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「匂いシグナル」で制御される口腔がん細胞の細胞融合誘導機構の解析
2022.4 - 2025.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(C)
荒木 啓吾, 川内 敬子
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Model system for nucleic acid-protein liquid-liquid phase separation
2021.4 - 2024.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(B)
Other External funds procured 【 display / non-display 】
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DNA高次構造によるレトロトランスポゾンLINE-1遺伝子の転写調節機構の解明とその制御法の探索
2024.1 - 2025.12
公益財団法人第一三共生命科学研究振興財団 研究助成金 財団等研究助成金
Authorship：principal_investigator
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低酸素ストレス耐性を獲得したがん細胞を標的とした治療薬の開発
2023.1 - 2024.3
公益財団法人テルモ生命科学振興財団 研究開発助成金 財団等研究助成金
Authorship：principal_investigator
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核内アクチン線維構造の制御による 新たながん治療創薬への挑戦
2018.4 - 2020.3
甲南学園 甲南学園平生太郎基金科学研究奨励助成金
Committee Memberships 【 display / non-display 】
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2024.12 健康長寿再生医療委員会 特定認定再生医療等委員会 委員
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2021.6 - 2023.9 日本学術会議 総合工学委員会科学的知見の創出に資する可視化分科会 細胞－身体可塑基盤からの自分を知り育てる科学的知見創出に資する可視化小委員会委員
Social Activities 【 display / non-display 】
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第25回健康長寿再生医療委員会 審査
Role(s)： Advisor
健康長寿再生医療委員会 2025.2