写真a

KAWAUCHI Keiko

Position

Associate Professor

External Link

Graduating School 【 display / non-display

  • Himeji Institute of Technology   Faculty of Science   Graduated

    1991.4 - 1995.3

Graduate School 【 display / non-display

  • 姫路工業大学大学院   理学研究科   生命科学専攻   Doctor's Course   Completed

    1998.4 - 2002.3

Campus Career 【 display / non-display

  • KONAN UNIVERSITY   Faculty of Frontiers of Innovative Research in Science and Technology   Faculty of Frontiers of Innovative Research in Science and Technology Department of Nanobiochemistry   Associate Professor

    2018.4

  • KONAN UNIVERSITY   Faculty of Frontiers of Innovative Research in Science and Technology   Faculty of Frontiers of Innovative Research in Science and Technology Department of Nanobiochemistry   Lecturer

    2014.4 - 2018.3

External Career 【 display / non-display

  • 特定国立研究開発法人理化学研究所   生命機能科学研究センター

    2017.4 - 2021.3

      More details

    Country:Japan

  • 日本医科大学   先端医学研究所

    2014.4

      More details

    Country:Japan

  • National University of Singapore   Mechanobiology Institute

    2010.5 - 2014.3

      More details

    Country:Singapore

  • 日本医科大学   老人病研究所

    2008.10 - 2011.12

      More details

    Country:Japan

  • 日本医科大学   老人病研究所

    2007.4 - 2008.9

      More details

    Country:Japan

display all >>

 

Papers 【 display / non-display

  • An anionic phthalocyanine decreases NRAS expression by breaking down its RNA G-quadruplex Reviewed

    Kawauchi K, Sugimoto W, Yasui T, Murata K, Itoh K, Takagi K, Tsuruoka T, Akamatsu K, Tateishi-Karimata H, Sugimoto N, Miyoshi D.

    Nature Commun.   9   2271   2018.6

     More details

    Joint Work

  • Intramolecular G-quadruplex-hairpin loop structure competition of a GC-rich exon region in the <i>TMPRSS2</i> gene

    Wataru Sugimoto, Natsuki Kinoshita, Minori Nakata, Tatsuya Ohyama, Hisae Tateishi-Karimata, Takahito Nishikata, Naoki Sugimoto, Daisuke Miyoshi, Keiko Kawauchi

    Chemical Communications   58 ( 1 )   48 - 51   2022

     More details

    Publisher:Royal Society of Chemistry (RSC)  

    We identified cytosine-rich regions adjacent to guanine-rich regions in the TMPRSS2 gene, which showed structural competition between a G-quadruplex and a hairpin loop. Furthermore, this competition significantly affected transcription efficiency.

    DOI: 10.1039/d1cc05523b

    researchmap

  • Anti-Malignant Effect of Tensile Loading to Adherens Junctions in Cutaneous Squamous Cell Carcinoma Cells. International journal

    Oleg Dobrokhotov, Masaki Sunagawa, Takeru Torii, Shinji Mii, Keiko Kawauchi, Atsushi Enomoto, Masahiro Sokabe, Hiroaki Hirata

    Frontiers in cell and developmental biology   9   728383 - 728383   2021

     More details

    Actomyosin contractility regulates various cellular processes including proliferation and differentiation while dysregulation of actomyosin activity contributes to cancer development and progression. Previously, we have reported that actomyosin-generated tension at adherens junctions is required for cell density-dependent inhibition of proliferation of normal skin keratinocytes. However, it remains unclear how actomyosin contractility affects the hyperproliferation ability of cutaneous squamous cell carcinoma (cSCC) cells. In this study, we find that actomyosin activity is impaired in cSCC cells both in vitro and in vivo. External application of tensile loads to adherens junctions by sustained mechanical stretch attenuates the proliferation of cSCC cells, which depends on intact adherens junctions. Forced activation of actomyosin of cSCC cells also inhibits their proliferation in a cell-cell contact-dependent manner. Furthermore, the cell cycle arrest induced by tensile loading to adherens junctions is accompanied by epidermal differentiation in cSCC cells. Our results show that the degree of malignant properties of cSCC cells can be reduced by applying tensile loads to adherens junctions, which implies that the mechanical status of adherens junctions may serve as a novel therapeutic target for cSCC.

    DOI: 10.3389/fcell.2021.728383

    PubMed

    researchmap

  • Photosensitizers Based on G-Quadruplex Ligand for Cancer Photodynamic Therapy. Reviewed

    Keiko Kawauchi, Ryoto Urano, Natsuki Kinoshita, Shin Kuwamoto, Takeru Torii, Yoshiki Hashimoto, Shinya Taniguchi, Mitsuki Tsuruta, Daisuke Miyoshi

    Genes   11 ( 11 )   2020.11

     More details

    Joint Work

    G-quadruplex (G4) is the non-canonical secondary structure of DNA and RNA formed by guanine-rich sequences. G4-forming sequences are abundantly located in telomeric regions and in the promoter and untranslated regions (UTR) of cancer-related genes, such as RAS and MYC. Extensive research has suggested that G4 is a potential molecular target for cancer therapy. Here, we reviewed G4 ligands as photosensitizers for cancer photodynamic therapy (PDT), which is a minimally invasive therapeutic approach. The photosensitizers, such as porphyrins, were found to be highly toxic against cancer cells via the generation of reactive oxidative species (ROS) upon photo-irradiation. Several porphyrin derivatives and analogs, such as phthalocyanines, which can generate ROS upon photo-irradiation, have been reported to act as G4 ligands. Therefore, they have been implicated as promising photosensitizers that can selectively break down cancer-related DNA and RNA forming G4. In this review, we majorly focused on the potential application of G4 ligands as photosensitizers, which would provide a novel strategy for PDT, especially molecularly targeted PDT (mtPDT).

    DOI: 10.3390/genes11111340

    PubMed

    researchmap

  • Novel Strategy of Photodynamic Therapy Targeting RAS mRNA with G-Quadruplex Ligands for Cancer Treatment Reviewed

    Takeru Torii, Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi

    Journal of Data Mining in Genomics & Proteomics   11 ( 2 )   2020.8

     More details

    Joint Work

display all >>

Books and Other Publications 【 display / non-display

  • 相分離生物学の全貌

    三好大輔, 川内敬子(DNA・RNAと相分離)

    現代化学増刊  2020.11 

     More details

  • がん研究読本 6

    川内敬子(RASによるがん悪性化に、p53を介したアクチン細胞骨格の変化がブレーキをかける道筋を解明!)

    がん研究分野の特性等を踏まえた支援活動総括支援活動班  2016 

     More details

  • ゼロからはじめるバイオ実験マスター3細胞培養トレーニング

    西方敬人, 川上純司, 藤井敏司, 長濱宏治, 川内敬子( Role: Joint author)

    学研メディカル集潤社  2015.3 

  • がん研究読本6

    大野茂男、千葉奈津子、川内敬子、駒田雅之、山本陽一朗、宮川隆、小原英幹、野島博、芳賀永、下野洋平、上野博夫、北爪しのぶ、佐谷秀行( Role: Joint author)

    がん研究分野の特性等を踏まえた支援活動総括支援活動班  2015 

  • 細胞培養トレーニング

    西方 敬人, 川上 純司, 藤井 敏司, 長濱 宏治, 川内 敬子

    学研メディカル秀潤社, 学研マーケティング (発売)  2015  ( ISBN:9784780909036

     More details

Review Papers (Misc) 【 display / non-display

display all >>

Presentations 【 display / non-display

  • RASによるがん悪性化機構の解明と制御法の開発

    川内敬子

    SJWS 新春シンポジウム2021  (オンライン)  日本女性科学者の会

     More details

    Event date: 2021.1

  • ワークショップ”Understanding of health and disease in cell, tissue, and organ systems based on cytoskeletal dynamics”

    川内敬子、吉野大輔、平田宏聡

    第43回 日本分子生物学会年会 

     More details

    Event date: 2020.12

  • DNA損傷応答における核内アクチンフィラメント構造に対するp53の役割

    川内敬子, 伊藤功彦, 杉本渉, 取井猛流, 岩根敦子, 平田宏聡

    第42回 日本分子生物学会年会 

     More details

    Event date: 2019.12

    researchmap

  • メカノバイオロジーの再生医療への展開

    川内敬子

    第9回 細胞再生医療研究会 

     More details

    Event date: 2019.8

    researchmap

  • DNA損傷応答におけるアクチンの役割

    川内敬子, 杉本渉, 伊藤功彦

    第41回 日本分子生物学会年会 

     More details

    Event date: 2018.11

    researchmap

display all >>

Academic Awards Received 【 display / non-display

  • 村尾育英会 学術賞

    2019.3   一般財団法人 村尾育英会  

    川内敬子

  • 神奈川難病財団研究奨励賞

    2009.12   神奈川難病財団  

    川内敬子

Grant-in-Aid for Scientific Research 【 display / non-display

  • Formation and function of nucleolus depending on rRNA G-quadruplexes

    2020.7 - 2022.3

    JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

      More details

  • Regulation of stress granule formation by RNA G-quadruplex

    2018.6 - 2020.3

    JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)

      More details

  • The role of nuclear actin filaments in cancer cells

    2018.4 - 2021.3

    JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(C)

      More details

Other External funds procured 【 display / non-display

  • 核内アクチン線維構造の制御による 新たながん治療創薬への挑戦

    2018.4

    甲南学園  甲南学園平生太郎基金科学研究奨励助成金

 

Social Activities 【 display / non-display

  • がん細胞の謎を解く~がんの予防と治療に向けて~

    Role(s): Lecturer

    JR東日本  東京オトナ大学  2019.12

     More details

  • 『がん』とは?

    Role(s): Lecturer

    東京海上日動火災保険(株)・甲南大学  公開講座  2017.11

     More details

  • ダイバーシティ研究環境整備と女性研究者の未来

    Role(s): Panelist

    日経グループ  日経ウーマノミクスプロジェクト  2017.5

     More details