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Natsumi Ueda, Hayato Okazaki, Akihiro Mikuma, Ayane Kunieda, Soma Kawashima, Takeru Torii, Keiko Kawauchi, Masatake Matsuoka, Tomohiro Onodera, Norimasa Iwasaki, Koji Nagahama
16 8847 2025年10月
担当区分：最終著者, 責任著者
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Effects of molecular crowding on the structure, folding, stability, and catalysis of RNA 招待あり 査読あり 国際誌
Shu-Ichi Nakano
Subcellular Biochemistry, (Macro)Molecular Crowding (Springer) 109 155 - 185 2025年10月
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Metal-organic framework-injectable hydrogel hybrid scaffolds promote accelerated angiogenesis for in vivo tissue engineering 査読あり
S. S. Islam, T. Dode, S. Kawashima, M. Fukuoka, T. Tsuruoka, K. Nagahama
RSC Adv. 15 32143 - 32154 2025年9月
担当区分：責任著者
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Functional analysis of histidine-dependent self-cleaving RNAs obtained by in vitro selection 査読あり
Nae Sakimoto, Shoichiro Tanaka, Riki Hatakenaka, Yoshie Yamaguchi-Miyazaki, Elisa Tomita-Sudo, Tomoka Akita, Taku Ishigaki, Shigenori Iwai, Junji Kawakami
ChemBioChem e202500411 2025年9月
担当区分：最終著者, 責任著者
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Metal–organic framework–injectable hydrogel hybrid scaffolds promote accelerated angiogenesis for in vivo tissue engineering 査読あり
Sobuj Shahidul Islam, Tatsuya Dode, Soma Kawashima, Myu Fukuoka, Takaaki Tsuruoka, Koji Nagahama
RSC Advances 15 32143 - 32154 2025年9月
担当区分：最終著者, 責任著者
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Hydrophilic ether-modified carboxybetaines: a molecular design approach for enzyme activation and stabilization 査読あり
Takuma Aoki, Koki Takagi, Yuichi Nakagawa, Kazuya Koumoto
Bulletin of the Chemical Society of Japan 98 2025年8月
担当区分：最終著者, 責任著者
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Sobuj Shahidul Islam,Tatsuya Dode,Soma Kawashima,Myu Fukuoka,Takaaki Tsuruoka andKoji Nagahama
Gels 11 ( 7 ) 514 2025年7月
担当区分：最終著者, 責任著者
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MOFs—Combining Fully Synthetic Injectable Hydrogel Scaffolds Exhibiting Higher Skeletal Muscle Regenerative Efficiency than Matrigel 査読あり
S. S. Islam, T. Dode, S. Kawashima, M. Fukuoka, T. Tsuruoka, K. Nagahama
Gles 11 514 2025年7月
担当区分：責任著者
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Madoka Suzuki, Keiko Kawauchi, Hiroaki Machiyama, Hiroaki Hirata, Shin'ichi Ishiwata, Hideaki Fujita
Biotechnology and Bioengineering 122 ( 7 ) 1929 - 1940 2025年7月
DOI： 10.1002/bit.28996
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Comprehensive analysis of stability and variability of DNA minimal i-motif structures 招待あり 査読あり
K. Ashida, A. Kitabayashi, K. Nishiyama, and S. Nakano
Molecules 30 1831 2025年4月
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One-Pot Synthesis and Immobilization of Gold Nanoparticles Using Peptidyl Microbeads 査読あり 国際誌
Shuhei Yoshida, Koki Yoshida, Taichi Isozaki, Maho Oura, Makoto Ozaki, Takaaki Tsuruoka, Kenji Usui
Molecules 30 1689 2025年4月
Gold nanoparticles (AuNPs) have surface plasmon resonance (SPR) and catalytic activity that are not found in bulk gold and have been studied in various fields. Among these, immobilization of AuNPs on various solid-phase substrates is known to produce stable catalytic activity and specific SPRs and research on the immobilization of AuNPs has been conducted actively. However, the conventional method requires the preparation and immobilization of AuNPs in separate processes, making it difficult to prepare immobilized AuNPs in a one-pot process. In this study, we attempted to synthesize and immobilize AuNPs using peptidyl beads, which are microbeads having immobilized a peptide capable of reducing gold ions. We successfully reduced Au ions from 0.5 to 1000 µM of HAuCl4 and immobilized them on peptidyl beads in the form of AuNPs. The immobilized AuNPs have a constant particle size independent of the HAuCl4 concentration. Furthermore, the peptidyl beads with AuNPs have catalytic activity. The quantity of the AuNPs on the peptidyl beads and, subsequently, the catalytic reaction rate of the sample, could be controlled. This study would also be expected to be applied to the immobilization of metallic nanomaterials other than AuNPs by modifying the peptide sequence.
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Liquid-liquid phase separation induced by i-motif DNA under molecular crowding conditions 招待あり 査読あり
Ryosuke Suzuki, Mitsuki Tsuruta, Sumit Shil, Kosei Morohashi, Keiko Kawauchi, Daisuke Miyoshia
Polymer J. 57 931 - 940 2025年4月
担当区分：責任著者
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One-Pot Synthesis and Immobilization of Gold Nanoparticles Using Peptidyl Microbeads 査読あり
Shuhei Yoshida, Koki Yoshida, Taichi Isozaki, Maho Oura, Makoto Ozaki, Takaaki Tsuruoka, Kenji Usui
Molecules 2025年4月
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Tendon Tissue Regeneration With Cell Orientation Using an Injectable Alginate-Cell Cross-linked Gel 査読あり
Jun Yamaguchi, Kentaro Homan, Tomohiro Onodera, Masatake Matsuoka, Shoutaro Arakawa, Natsumi Ueda, Nana Kawate, Takayuki Nonoyama, Yoshinori Katsuyama, Koji Nagahama, Mitsuru Saito, Norimasa Iwasaki
The American Journal of Sports Medicine 2025年3月
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Three- and four-stranded nucleic acid structures and their ligands 招待あり 査読あり
Yoshiki Hashimoto, Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, and Daisuke Miyoshi
RSC Chemical Biology 6 466 - 491 2025年3月
担当区分：責任著者
DOI： 10.1039/D4CB00287C
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ケミカルバイオロジーの冒険（１２） 核酸を標的とした低分子・中分子化合物 招待あり
堂野主税、勝田陽介、三好大輔
現代化学 648 32 - 39 2025年2月
担当区分：最終著者
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Roles of Loop Region in Folding Kinetics and Transcription Inhibition of DNA G-Quadruplexes 査読あり
Minori Nakata, Naoki Kosaka, Keiko Kawauchi, Daisuke Miyoshi
64 609 - 619 2025年2月
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Development of a New Structural Transformation Reaction in Cr-Based MOFs Induced by Fluoride 査読あり
Shintaro Tanaka, Ryoya Miyata, Reon Teramoto, Junya Sugimoto, Takaaki Tsuruoka, Kensuke Akamatsu, Yohei Takashima
Chem. Asian J e202401717 2025年2月
担当区分：最終著者, 責任著者
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Development of detection system for lead ions in mixture solutions using UV-Vis measurements with peptide immobilized microbeads 査読あり 国際誌
Shuhei Yoshida, Koki Yoshida, Yoshio Hamada, Takaaki Tsuruoka, Kenji Usui
Scientific Reports 15 3249 2025年1月
Environmental pollution caused by heavy metals are problems worldwide. In particular, pollution and poisoning by lead ions (Pb2+) continue to be common and serious problems. Hence, there is a need for a widely usable method to easily detect Pb2+ from solutions containing organic materials from environmental water such as seas, ponds, etc. Here, we established a system to easily detect Pb2+ from such mixture solutions using Pb2+ binding peptide immobilized beads (peptidyl beads) and ultraviolet (UV) absorption measurements. This method could detect Pb2+ at low concentrations equivalent to inductively coupled plasmon-atomic emission spectroscopy (ICP-AES). Using the detected values to create a calibration curve, it was found that there was a positive correlation between the concentration of Pb2+ and absorbance, which also made it possible to quantify sub-µM Pb2+ in the solutions. Furthermore, Pb2+ was detected and quantified under mixed conditions of environmental water such as seas, rivers, and ponds. This method is expected to become a versatile and easy-to-use Pb2+ detection method for end-users worldwide.
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Complex and Non-sequential Current Signatures of a β-Hairpin Peptide Confined in a Nanopore 国際共著 国際誌
Misa Yamaji, Mauro Chinappi, Blasco Morozzo della Rocca, Kenji Usui, Ryuji Kawano
Analytical Chemistry 97 2044 - 2051 2025年1月
出版者・発行元：American Chemical Society (ACS)
Nanopore sensing is widely used for single-molecule detection, originally applied to nucleic acids and now extended to protein sensing. Our study focuses on the complex conformational changes of peptides in nanopores, which may have implications for peptide fingerprinting and protein identification. Specifically, we investigated the interaction of a β-hairpin peptide (SV28) within an α-hemolysin (αHL) nanopore. Our experiments revealed that SV28 is captured via dielectrophoresis and exhibits long dwell times within the nanopore, leading to multiple current blockade levels. Unlike DNA hairpins, the peptide showed non-sequential transitions among four distinct blockade levels. This complex behavior indicates that the peptide dynamics in nanopores cannot be simply modeled along a single reaction coordinate. Our findings provide insights into peptide-nanopore interactions, which are potentially useful for developing nanopore-based peptide identification technologies.