Papers 【 display / non-display 】
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Microwave-induced modulation of intracellular distribution of peptides based on mitochondrial targeting sequences Reviewed International journal
Fumihiro Kayamori, Takuto Kariya, Momo Hirata, Yonejiro Arimoto, Tamaki Endoh, Nobuhiro Nakanishi & Kenji Usui
Scientific Reports 2026.6
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A Comparative Study on the Relative Quantification of Oligonucleotide Impurities Using Several Different Types of Liquid Chromatography-Mass Spectrometers at Analytical Facilities of Multiple Institutions Reviewed
Ryutaro Takamine, Takao Yamaguchi, Kenji Hirose, Mitsuaki Sekiguchi, Kaoru Karasawa, Noriyuki Iwasaki, Kentaro Takahara, Hiroshi Sezaki, Yoshihiro Hayakawa, Hiroyuki Togawa, Takashi Okubo, Hiroo Nagano, Amane Sakurai, Nae Sakimoto, Elisa Tomita-Sudo, Tokuyuki Yoshida, Takao Inoue, Satoshi Obika, Junji Kawakami
Pharmaceutical and Medical Device Regulatory Science 57 ( 3 ) 2026.6
Authorship：Last author, Corresponding author
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Cy3-Conjugated Biocompatible Polymer Nanoparticles for Long-Term Mitochondrial Imaging Reviewed
ChemBioChem ChemBioChem Research Article Full Access Cy3-Conjugated Biocompatible Polymer Nanoparticles for Long-Term Mitochondrial Imaging Souma Kawashima, Mitsuo Inui, Izumi Takaba, Haruka Taniguchi, Koji Nagahama
ChemBioChem 27 ( 8 ) e70323 2026.4
Authorship：Last author, Corresponding author
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Impact of Defects and Particle Size on the Reversible Structural Transformation between Metal-Organic Frameworks Reviewed
Shintaro Tanaka, Yuta Kanao, Takaaki Tsuruoka, Kensuke Akamatsu, Yohei Takashima
Chem. Mater. 38 2669 - 2679 2026.3
Authorship：Last author, Corresponding author
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Loss of p53 Provokes NF-κB-Dependent Disruption of Nucleolar Cap and Nucleoplasmic Redistribution of Fibrillarin During Nucleolar Stress. Reviewed
Torii T, Sumida M, Kobayashi A, Goto T, Suzuki R, Kuwamoto S, Nakajima W, Sugimoto W, Takeuchi K, Tanaya Y, Tera M, Tanaka N, Hirata H, Tateishi-Karimata H, Nishikata T, Homma MK, Miyoshi D, Kawauchi K.
Biomolecules. 16 ( 2 ) 296 2026.2
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Loss of p53 Provokes NF-κB-Dependent Disruption of Nucleolar Cap and Nucleoplasmic Redistribution of Fibrillarin During Nucleolar Stress Invited Reviewed
Torii T, Sumida M, Kobayashi A, Goto T, Suzuki R, Kuwamoto S, Nakajima W, Sugimoto W, Takeuchi K, Tanaya Y, Tera M, Tanaka N, Hirata H, Tateishi-Karimata H, Nishikata T, Kato Homma M, Miyoshi D, Kawauchi K
Biomolecules ( 16 ) 296 2026.2
Authorship：Last author, Corresponding author
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Electroplating of Ni using a polyelectrolyte membrane: effect of deposition conditions on interfacial ion-transport kinetics and current efficiency Reviewed
Shunsuke Yamada, Yohei Takashima, Takaaki Tsuruoka, Kensuke Akamatsu
Electrochemistry 94 037006 2026.2
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Development of a Microwave-Assisted Method for Enhancing the Cellular Uptake of Arginine-Rich Peptides Reviewed International journal
Fumihiro Kayamori, Momo Hirata, Takuto Kariya, Yonejiro Arimoto, Ryuji Osawa, Mami Ishikawa, Tamaki Endoh, Nobuhiro Nakanishi, Kenji Usui
Processes 14 ( 3 ) 497 2026.1
Joint Work
Authorship：Last author, Corresponding author
Cell-penetrating peptides (CPPs) have been extensively explored as tools for the intracellular delivery of diverse molecular cargoes. Although substantial progress has been made in elucidating their uptake mechanisms and sequence-dependent functions, limitations in cellular internalization efficiency remain a major challenge, hindering their broader biomedical application. To address this issue, the present study investigated whether microwave (MW) irradiation at 2.45 GHz can enhance CPP-mediated delivery. Using confocal laser scanning microscopy and fluorescence spectroscopy, we examined the effects of MW irradiation on the cellular uptake of arginine-rich peptides. Our results suggested that MW irradiation enhanced the cellular uptake of the peptides. These findings imply that CPP-mediated delivery assisted by MW irradiation is an effective method for improving intracellular transport and may open new avenues for the development of advanced drug delivery systems.
DOI： 10.3390/pr14030497
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Development of a Microwave-Assisted Method for Enhancing the Cellular Uptake of Arginine-Rich Peptides Reviewed
14 ( 3 ) 2026.1
DOI： 10.3390/pr14030497
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Investigation of the physicochemical and functional properties of poly(2-methacryloyloxyethyl phosphorylcholine)-conjugated aptamers. International journal
Seojung Cho, Jumpei Morimoto, Yutaro Saito, Yukiko Nagai, Asuka Sakata, Keitaro Yoshimoto, Mitsuki Tsuruta, Daisuke Miyoshi, Shinsuke Sando
Biomaterials science 14 ( 1 ) 232 - 239 2026.1
Polymer conjugation is a common strategy to improve the pharmacokinetics of aptamers, yet its effects on aptamer properties are incompletely understood. Poly(ethylene glycol) (PEG) is the most widely used polymer for this purpose, but concerns about anti-PEG immune responses have prompted interest in alternative polymers. We previously reported that conjugation with the zwitterionic polymer poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) significantly prolongs the circulation time of a DNA aptamer while avoiding anti-PEG antibody recognition. In this study, we evaluated the physicochemical and functional consequences of PMPC conjugation of aptamers. Biophysical analyses suggested that the secondary structure and target-binding affinity of the aptamer were preserved, while functional consequences upon PMPC conjugation varied with the targets. The activity of a membrane receptor-targeting aptamer partially decreased, likely due to spatial constraints around the cell membrane, while RB005, targeting soluble activated coagulation factor IX, retained its full activity. In addition, PMPC conjugation significantly prolonged the in vivo plasma retention of RB005. By elucidating the effects of PMPC on aptamer properties and introducing another example that further supports the general applicability of PMPC conjugation in enhancing aptamer pharmacokinetics, these findings support PMPC as a promising alternative to PEG.
DOI： 10.1039/d5bm01078k
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Current Trends in Liquid Chromatography-Mass Spectrometry Analysis of Small Interfering RNAs: A Short Review Reviewed
Hiroyuki Togawa, Takao Yamaguchi, Junji Kawakami, Satoshi Obika
Journal of Chromatography A 1766 466550 2026.1
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Theoretical calculation of the amounts of nucleotide deletion impurities in oligonucleotides Reviewed
Yasunori Uchida, Tokuyuki Yoshida, Emi Saito, Hirokazu Nankai, Kaoru Karasawa, Chie Inagaki, Noriyuki Iwasaki, Daisuke Higo, Kentaro Takahara, Takashi Osawa, Takao Yamaguchi, Kosuke Ito, Junji Kawakami, Satoshi Obika, Takao Inoue
International Journal of Pharmaceutical 687 126364 2026.1
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Effects of N–1-mer impurities in antisense oligonucleotides on the target RNA suppression Reviewed
Tomoka Akita, Elisa Tomita-Sudo, Renshin Sano, Nae Sakimoto, Shigenori Iwai, Hirokazu Nankai, Satoshi Obika, Takao Inoue, Junji Kawakami
Nucleic Acid Therapeutics 2026
Authorship：Last author, Corresponding author
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Position-dependent stabilization of DNA/RNA duplexes by site-specific incorporation of LNA nucleosides Reviewed
Elisa Tomita-Sudo, Tomoka Akita, Nae Sakimoto, Saori Tahara-Takamine, Renshin Sano, Shigenori Iwai, Junji Kawakami
Journal of Nucleic Acids 2026
Authorship：Last author, Corresponding author
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Synthesis of Biocompatible Gold Nanoparticles for Photothermal Therapy by Mineralization Using Peptides Reviewed International journal
Shuhei Yoshida, Koki Yoshida, Yoshiki Shitamukai, Makoto Ozaki, Takaaki Tsuruoka, Kenji Usui
RSC Advances 15 48521 - 48528 2025.12
Joint Work
Authorship：Corresponding author
DOI： https://doi.org/10.1039/d5ra08717a
Other Link： https://pubs.rsc.org/en/content/articlepdf/2025/ra/d5ra08717a
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Phase Separation of RX Repeat Peptides with Nucleic Acids. International journal
Sumit Shil, Mitsuki Tsuruta, Ryosuke Suzuki, Yoshiki Hashimoto, Takeru Torii, Shinya Taniguchi, Tomohiro Umetani, Keiko Kawauchi, Daisuke Miyoshi
Chemistry, an Asian journal 20 ( 23 ) e00805 2025.12
Biomolecular liquid-liquid phase separation (LLPS) plays a crucial role in organizing membraneless cellular compartments, which regulate a wide variety of cellular processes. A key molecular mechanism underlying LLPS of nucleic acids involves G-quadruplex (G4) structures of DNA and RNA interacting with intrinsically disordered proteins, particularly arginine and glycine (RGG/RG) rich proteins. The role of arginine residues in LLPS has been studied extensively, whereas few studies have focused on the role of the another frequently occurring residues, glycine. Here, we systematically investigated the contribution of G residues by substituting them with alanine (A), proline (P), valine (V), and tyrosine (Y) residues, generating a series of RX repeat peptides. Turbidity and microscopy assays with DNA oligonucleotides forming G4, duplex, as well as random coil, showed that RP and RA-peptides enhanced LLPS with G4 DNA, by comparing RG-peptide. In contrast, RY promoted liquid-solid phase separation (LSPS) with the G4 DNA, although it underwent LLPS with the random coil and duplex DNAs. In addition, RV-peptide formed aggregates even in the absence of any DNA. These results demonstrate that side-chain size, hydrophobicity, and aromaticity are critical factors for the LLPS and LSPS capability and selectivity with DNA forming various secondary structures. This study provides mechanistic insights into protein-nucleic acid LLPS and LSPS and guides the rational design peptides to undergo LLPS but not LSPS with nucleic acids.
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Controlling Film Formation of Ag-Chalcogenate Coordination Polymer via Ag Ion-Doped Polymer Substrates Reviewed
Takaaki Tsuruoka, Riko Oishi, Yohei Takashima, Kensuke Akamatsu
Inorganics 13 396 2025.11
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Natsumi Ueda, Hayato Okazaki, Akihiro Mikuma, Ayane Kunieda, Soma Kawashima, Takeru Torii, Keiko Kawauchi, Masatake Matsuoka, Tomohiro Onodera, Norimasa Iwasaki, Koji Nagahama
Nature Communications 16 8847 2025.10
Authorship：Last author, Corresponding author
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In vivo mechano-tissue engineering by hydrogels capable of transmitting intercellular mechanical stress Reviewed
Ueda N, Okazaki O, Mikuma A, Kunieda A, Kawashima S, Torii T, Kawauchi K, Matsuoka M, Onodera T, Iwasaki N, Nagahama K.
Nature Communications 16 8847 2025.10
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Effects of molecular crowding on the structure, folding, stability, and catalysis of RNA Invited Reviewed International journal
Shu-Ichi Nakano
Subcellular Biochemistry, (Macro)Molecular Crowding (Springer) 109 155 - 185 2025.10
Single Work
Authorship：Lead author, Corresponding author Publisher：Springer