Papers 【 display / non-display 】
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Liquid-liquid phase separation induced by i-motif DNA under molecular crowding conditions Invited Reviewed
Ryosuke Suzuki, Mitsuki Tsuruta, Sumit Shil, Kosei Morohashi, Keiko Kawauchi, Daisuke Miyoshia
Polymer J. 2025.4
Authorship：Corresponding author
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Tendon Tissue Regeneration With Cell Orientation Using an Injectable Alginate-Cell Cross-linked Gel Reviewed
Jun Yamaguchi, Kentaro Homan, Tomohiro Onodera, Masatake Matsuoka, Shoutaro Arakawa, Natsumi Ueda, Nana Kawate, Takayuki Nonoyama, Yoshinori Katsuyama, Koji Nagahama, Mitsuru Saito, Norimasa Iwasaki
The American Journal of Sports Medicine 2025.3
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Three- and four-stranded nucleic acid structures and their ligands Invited Reviewed
Yoshiki Hashimoto, Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, and Daisuke Miyoshi
RSC Chemical Biology 6 466 - 491 2025.3
Authorship：Corresponding author
DOI： 10.1039/D4CB00287C
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ケミカルバイオロジーの冒険（１２） 核酸を標的とした低分子・中分子化合物 Invited
堂野主税、勝田陽介、三好大輔
現代化学 648 32 - 39 2025.2
Authorship：Last author
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Development of a New Structural Transformation Reaction in Cr-Based MOFs Induced by Fluoride Reviewed
Shintaro Tanaka, Ryoya Miyata, Reon Teramoto, Junya Sugimoto, Takaaki Tsuruoka, Kensuke Akamatsu, Yohei Takashima
Chem. Asian J e202401717 2025.2
Authorship：Last author, Corresponding author
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Development of detection system for lead ions in mixture solutions using UV-Vis measurements with peptide immobilized microbeads
Shuhei Yoshida, Koki Yoshida, Yoshio Hamada, Takaaki Tsuruoka, Kenji Usui
Scientific Reports 15 3249 2025.1
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Roles of Loop Region in Folding Kinetics and Transcription Inhibition of DNA G-Quadruplexes Reviewed
Minori Nakata, Naoki Kosaka, Keiko Kawauchi, Daisuke Miyoshi
Biochemistry 64 609 - 619 2025.1
Authorship：Corresponding author
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Sequence-Specific Free Energy Changes in DNA/RNA Induced by a Single LNA-T Modification in Antisense Oligonucleotides Reviewed
Elisa Tomita-Sudo, Tomoka Akita, Nae Sakimoto, Saori Tahara-Takamine, Junji Kawakami
International Journal of Molecular Sciences 25 13240 - 13240 2024.12
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L-Histidine Modulates the Catalytic Activity and Conformational Changes of the HD3 Deoxyribozyme Reviewed
Nae Sakimoto, Hirofumi Imanaka, Elisa Tomita-Sudo, Tomoka Akita, Junji Kawakami
genes 15 1481 2024.11
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Detection of Conformational Changes of G4 Aptamer in the Presence of Different Cations using Calorimetry Invited Reviewed
Elisa Tomita-Sudo, Taku Ishigaki, Masako Hirose, Maki Kato, and Junji Kawakami
Netsu Sokutei 51 ( 4 ) 148 - 152 2024.10
Authorship：Last author
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NRAS DNA G-quadruplex-targeting molecules for sequence-selective enzyme inhibition Invited Reviewed
Yoshiki Hashimoto, Hiroki Kubo, Keiko Kawauchi and Daisuke Miyoshi
Chem. Commun. 2024.10
Authorship：Corresponding author
DOI： 10.1039/D4CC03753G
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The role of cytosine methylation in regulating the topology and liquid-liquid phase separation of DNA G-quadruplexes Reviewed
Mitsuki Tsuruta, Sumit Shil, Shinya Taniguchi, Keiko Kawauchi and Daisuke Miyoshi
Chem. Sci. 16 4213 - 4225 2024.10
Authorship：Corresponding author
DOI： 10.1039/D4SC06959E
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Structural transformation of silver(I)-thiolate coordination plymer film at solid-liquid interfaces Reviewed International coauthorship
Myu Fukuoka, Yohei Takashima, Kensuke Akamatsu, Aude Demessence, Takaaki Tsuruoka
CrystEngComm 26 6269 - 6273 2024.10
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Factors Affecting Liquid-Liquid Phase Separation of RGG Peptides with DNA G-Quadruplex Invited Reviewed
Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi, Daisuke Miyoshi
ChemMedChem 2024.9
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Bioinformatic Analysis of Actin-Binding Proteins in the Nucleolus During Heat Shock Invited Reviewed
Shinya Taniguchi, Takeru Torii ,Toshiyuki Goto, Kohei Takeuchi, Rine Katsumi, Mako Sumida, Sunmin Lee, Wataru Sugimoto, Masaya Gessho, Katsuhiko Itoh, Hiroaki Hirata, Junji Kawakami, Daisuke Miyoshi and Keiko Kawauchi
Genes 15 ( 12 ) 1580 2024.9
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Non-canonical olfactory pathway activation induces cell fusion of cervical cancer cells. Reviewed
Keigo Araki, Takeru Torii, Kohei Takeuchi, Natsuki Kinoshita, Ryoto Urano, Rinka Nakajima, Yaxuan Zhou, Tokuo Kobayashi, Tadayoshi Hanyu, Kiyoshi Ohtani, Kimiharu Ambe, Keiko Kawauchi
Neoplasia 57 101044 2024.8
Authorship：Last author
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Quantitative Effects of the Loop Region on Topology, Thermodynamics, and Cation Binding of DNA G-quadruplexes Reviewed
ACS Omega 9 35028 - 35036 2024.7
Authorship：Last author, Corresponding author
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Development of a CaCO3 Precipitation Method Using a Peptide and Microwaves Generated by a Magnetron Reviewed
Fumihiro Kayamori, Hiroyuki Togashi, Natsumi Endo, Makoto Ozaki, Kan Hirao, Yonejiro Arimoto, Ryuji Osawa, Takaaki Tsuruoka, Takahito Imai, Kin-ya Tomizaki, Tomohiro Umetani, Nobuhiro Nakanishi, Kenji Usui
Processes 12 ( 7 ) 1327 - 1327 2024.6
Publisher：MDPI AG
Microwave applications, such as microwave ovens and mobile phones, are ubiquitous and indispensable in modern society. As the utilization of microwave technology is becoming more widespread, the effects of microwaves on living organisms and physiological processes have received increased attention. This study aimed to investigate the effects of microwaves on calcium carbonate biomineralization as a model biochemical process. A magnetron oscillator was used to generate 2450 MHz microwaves because magnetrons are relatively inexpensive and widespread. We conducted transmission electron microscopy (TEM), atomic force microscopy (AFM), TEM-electron energy-loss spectroscopy (EELS), dynamic light scattering (DLS), and high-performance liquid chromatography (HPLC) measurements to analyze the calcium carbonate precipitates. Our findings showed the formation of string-like precipitates of calcium carbonate upon microwave irradiation from one direction, similar to those obtained using a semiconductor oscillator, as reported previously. This implied that the distribution of the frequency had little effect on the morphology. Furthermore, spherical precipitates were obtained upon microwave irradiation from two directions, indicating that the morphology could be controlled by varying the direction of microwave irradiation. Magnetrons are versatile and also used in large-scale production; thus, this method has potential in medical and industrial applications.
DOI： 10.3390/pr12071327
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Development of a CaCO3 Precipitation Method Using a Peptide and Microwaves Generated by a Magnetron Reviewed
Fumihiro Kayamori Hiroyuki Togashi Natsumi Endo Makoto Ozaki Kan Hirao Yonejiro Arimoto Ryuji Osawa Takaaki Tsuruoka Takahito Imai Kin-ya Tomizaki Tomohiro Umetani Nobuhiro Nakanishi Kenji Usui
Processes 12 ( 7 ) 1327 2024.6
Microwave applications, such as microwave ovens and mobile phones, are ubiquitous and indispensable in modern society. As the utilization of microwave technology is becoming more widespread, the effects of microwaves on living organisms and physiological processes have received increased attention. This study aimed to investigate the effects of microwaves on calcium carbonate biomineralization as a model biochemical process. A magnetron oscillator was used to generate 2450 MHz microwaves because magnetrons are relatively inexpensive and widespread. We conducted transmission electron microscopy (TEM), atomic force microscopy (AFM), TEM-electron energy-loss spectroscopy (EELS), dynamic light scattering (DLS), and high-performance liquid chromatography (HPLC) measurements to analyze the calcium carbonate precipitates. Our findings showed the formation of string-like precipitates of calcium carbonate upon microwave irradiation from one direction, similar to those obtained using a semiconductor oscillator, as reported previously. This implied that the distribution of the frequency had little effect on the morphology. Furthermore, spherical precipitates were obtained upon microwave irradiation from two directions, indicating that the morphology could be controlled by varying the direction of microwave irradiation. Magnetrons are versatile and also used in large-scale production; thus, this method has potential in medical and industrial applications.
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Inhibition of RNA phosphodiester backbone cleavage in the presence of organic cations of different sizes Invited Reviewed International journal
S. Yoshioka, A. Doi, and S. Nakano
ChemBioChem 25 ( 13 ) e202400276 2024.5
Joint Work
Authorship：Last author, Corresponding author Publisher：Wiley
Living cells contain various types of organic cations that may interact with nucleic acids. In order to understand the nucleic acid–binding properties of organic cations of different sizes, we investigated the ability of simple organic cations to inhibit the RNA phosphodiester bond cleavage promoted by Mg2+, Pb2+, and RNA-cleaving serum proteins. Kinetic analysis using chimeric DNA–RNA oligonucleotides showed that the cleavage at ribonucleotide sites was inhibited in the presence of monovalent cations comprising alkyl chains or benzene rings. The comparison of the cleavage rates in the presence of quaternary ammonium and phosphonium ions indicated that the steric hindrance effect of organic cations on their binding to the RNA backbone is significant when the cation size is larger than the phosphate–phosphate distance of a single-stranded nucleic acid. The cleavage inhibition was also observed for ribonucleotides located in long loops but not in short loops of oligonucleotide structures, indicating less efficient binding of bulky cations to structurally constrained regions. These results reveal the unique nucleic acid–binding properties of bulky cations distinct from those of metal ions.