Papers - MIYOSHI Daisuke
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Quantification of the concentration in a droplet formed by liquid–liquid phase separation of G-quadruplex-forming RNA Reviewed
Kohei Yokosawa, Mitsuki Tsuruta, Shinji Kajimoto, Naoki Sugimoto, Daisuke Miyoshi, Takakazu Nakabayashi
Chem. Phys. Lett. 2023.6
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The iron chelator deferriferrichrysin induces paraptosis via extracellular signal-related kinase activation in cancer cells Invited Reviewed
Natsuki Kinoshita, Masaya Gessho, Takeru Torii, Yukako Ashida, Minori Akamatsu, Alvin Kunyao Guo, Sunmin Lee, Tatsuya Katsuno 3, Wataru Nakajima Yemima Budirahardja, Daisuke Miyoshi, Takehiko Todokoro, Hiroki Ishida, Takahito Nishikata, Keiko Kawauchi
Genes Cells 2023.6
DOI： 10.1111/gtc.13053
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Biomolecular Liquid–Liquid Phase Separation for Biotechnology Invited Reviewed
Sumit Shil, Mitsuki Tsuruta, Keiko Kawauchi and Daisuke Miyoshi
BioTech 12 ( 2 ) 26 2023.3
Authorship：Corresponding author
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Simple and fast screening for structure-selective G-quadruplex ligands Reviewed
Yoshiki Hashimoto, Yoshiki Imagawa, Kaho Nagano, Ryuichi Maeda, Naho Nagahama, Takeru Torii, Natsuki Kinoshita, Nagisa Takamiya, Keiko Kawauchi, Hisae Tatesishi-Karimata, Naoki Sugimoto and Daisuke Miyoshi
Chem. Commun. 59 4891 - 4894 2023.2
Authorship：Corresponding author
DOI： 10.1039/D3CC00556A
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Controlling liquid–liquid phase separation of G-quadruplex-forming RNAs in a sequence-specific manner Reviewed
Mitsuki Tsuruta, Takeru Torii, Kazuki Kohata, Keiko Kawauchi, Hisae Tateishi-Karimata, Naoki Sugimoto and Daisuke Miyoshi
Chem. Commun. 2022.11
DOI： 10.1039/D2CC04366A
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細胞内環境で安定化される核酸非構造を狙った分子標的薬の開発 Invited
川内敬子・橋本佳樹・杉本 渉・三好大輔
バイオマテリアル = Journal of Japanese Society for Biomaterials : 生体材料 40 ( 3 ) 206 - 211 2022.7
Authorship：Corresponding author
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核酸の化学修飾と構造や相分離能の相関 Invited
鶴田 充生・取井 猛流・杉本 渉・川内 敬子・三好 大輔
月刊「細胞」（ニューサイエンス社） 53 ( 14 ) 910 - 913 2022.6
Authorship：Last author, Corresponding author
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Significant structural change in human c-Myc promoter G-quadruplex Reviewed International coauthorship
Nikita Kundu, Taniya Sharma, Sarvpreet Kaur, Mamta Singh, Vinit Kumar, Uttam Sharma, Aklank Jain, Jadala Shankaraswamy, Daisuke Miyoshi, Sarika Saxena
RSC Adv. 12 7594 - 7604 2022.4
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Intramolecular G-quadruplex-hairpin loop structure competition of a GC-rich exon region in the TMPRSS2 gene Reviewed
Chem. Commun. 58 48 - 51 2022.1
Authorship：Corresponding author
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rDNAおよびrRNAが形成する四重らせん構造による核小体機能の制御 Invited
川内敬子、取井猛流、月生雅也、木下菜月、三好大輔
月刊細胞 54 ( 12 ) 36 - 39 2022
Authorship：Corresponding author
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核酸の四重らせん構造を狙った治療薬の展望 Invited
37 ( 4 ) 52 - 55 2022
Authorship：Corresponding author
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Detection of Intracellular Reactive Oxidative Species Using the Fluorescent Probe Hydroxyphenyl Fluorescein Invited Reviewed
Wataru Sugimoto, Daisuke Miyoshi, Keiko Kawauchi
2274 207 - 215 2021.5
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がん関連RNAを破壊する分子標的型光線力学療法 Invited Reviewed
杉本 渉・橋本 佳樹・川内 敬子・三好 大輔
BIO Clinica 36 ( 5 ) 65 - 67 2021.5
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Combined Effects of Methylated Cytosine and Molecular Crowding on the Thermodynamic Stability of DNA Duplexes Invited Reviewed
Combined Effects of Methylated Cytosine, Molecular Crowding, on, the Thermodynamic Stability of, DNA Duplexes
Int. J. Mol. Sci. 22 ( 2 ) 947 2021.1
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Photosensitizers Based on G-Quadruplex Ligand for Cancer Photodynamic Therapy Invited International journal
Keiko Kawauchi, Ryoto Urano, Natsuki Kinoshita, Shin Kuwamoto, Takeru Torii, Yoshiki Hashimoto, Shinya Taniguchi, Mitsuki Tsuruta, Daisuke Miyoshi
Genes 11 ( 11 ) 1340 2020.11
Joint Work
G-quadruplex (G4) is the non-canonical secondary structure of DNA and RNA formed by guanine-rich sequences. G4-forming sequences are abundantly located in telomeric regions and in the promoter and untranslated regions (UTR) of cancer-related genes, such as RAS and MYC. Extensive research has suggested that G4 is a potential molecular target for cancer therapy. Here, we reviewed G4 ligands as photosensitizers for cancer photodynamic therapy (PDT), which is a minimally invasive therapeutic approach. The photosensitizers, such as porphyrins, were found to be highly toxic against cancer cells via the generation of reactive oxidative species (ROS) upon photo-irradiation. Several porphyrin derivatives and analogs, such as phthalocyanines, which can generate ROS upon photo-irradiation, have been reported to act as G4 ligands. Therefore, they have been implicated as promising photosensitizers that can selectively break down cancer-related DNA and RNA forming G4. In this review, we majorly focused on the potential application of G4 ligands as photosensitizers, which would provide a novel strategy for PDT, especially molecularly targeted PDT (mtPDT).
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TakahiroYamauchi, Takafumi Kubodera, Daisuke Miyoshi, Naoki Sugimoto, Shuji Hirohata
Journal of Bioscience and Bioengineering 2020.10
Joint Work
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Photodynamic therapy targeting RAS mRNA
Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi
Medical Science Digest 46 ( 9 ) 26 - 27 2020.7
Joint Work
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Novel Strategy of Photodynamic Therapy Targeting RAS mRNA with G-Quadruplex Ligands for Cancer Treatment Invited Reviewed
Takeru Torii, Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi
Journal of Data Mining in Genomics & Proteomics 2020.6
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RNA Phase Separation-Mediated Direction of Molecular Trafficking Under Conditions of Molecular Crowding Invited Reviewed
Kazuki Kohata, Daisuke Miyoshi
Biophys. Rev. 12 669 - 676 2020.5
Joint Work
Living cells are highly crowded with large and small biomolecules. The total concentration of biomolecules can reach 400 mg/ml, and 40% of the cell volume is occupied by biomolecules. Droplet formation in cells via liquid-liquid phase separation may play a role in controlling biochemical reactions in this complex molecular environment. Liquid-liquid phase separation generally involves nucleic acids and proteins as anionic and cationic components, respectively. Significant characteristics of droplets, which make them different from protein aggregation or fibril formation, are reversibility of formation and responsiveness to the molecular environment. In this review, we quantitatively describe the molecular environment inside cells and droplets that participate in controlling central dogma reactions. Finally, we discuss the importance of droplets under conditions of molecular crowding within living cells.
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Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA Reviewed
H. Tateishi-Karimata, D. Banerjee, T. Ohyama, S. Matsumoto, D. Miyoshi, S. Nakano, and N. Sugimoto
Biochemical and Biophysical Research Communications 525 ( 1 ) 177 - 183 2020.4
Joint Work