論文 - 川上 純司
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Comparison of Kinetic and Thermodynamic Analyses for Duplex Formation
E. Tomita, M. Sumimoto, S. Itoh, M. Takashima, and J. Kawakami
Proc. 42nd Intl. Symp. Nucleic Acid Chemistry 142 - 143 2015年
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Curcumisome nanovesicles generated by self-assembly of curcumin amphiphiles toward cancer theranostics
Koji Nagahama, Takayuki Kumano, Naho Oyama, Junji Kawakami
BIOMATERIALS SCIENCE 3 ( 12 ) 1566 - 1578 2015年
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出版者・発行元:ROYAL SOC CHEMISTRY
Curcumin (CCM) is an important molecule for achieving cancer theranostics because CCM is a naturally-occurring biocompatible material that exhibits both anticancer activity and strong fluorescence property that can be used for bio-imaging. However, CCM has never been utilized in clinical trials due to its extremely low water solubility, its rapid hydrolysis in aqueous conditions at neutral pH, and its low cellular uptake into cancer cells. Taking advantage of the strong hydrophobicity, p-conjugated frameworks, and ketone and enol groups that generate hydrogen bonds in CCM, we herein fabricated novel CCM-based biodegradable nanovesicles, which we termed as "curcumisome", through the self-assembly of amphiphilic CCM-poly(ethylene glycol) conjugates in aqueous media to develop multifunctional nanobiomaterials for use in cancer theranostics. A high CCM loading content in the curcumisomes was achieved, and the curcumisomes showed high water dispersibility with improved hydrolysis resistance. Importantly, the curcumisomes were effectively internalized into cancer cells and exhibited strong fluorescence for a long period, which is favorable for cancer cell imaging, although only a small amount of the curcumisomes penetrated into normal cells and showed very weak fluorescence. Moreover, curcumisomes effectively induced apoptosis of cancer cells. Thus, curcumisomes may act as multifunctional nanobiomaterials for the development of CCM-based cancer theranostics.
DOI: 10.1039/c5bm00212e
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Thermodynamic Features Provided by an ENA Introduction to Nucleic Acid Drugs
E. Tomita, M. Sumimoto, and J. Kawakami
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 188 - 189 2014年11月
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担当区分:筆頭著者
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Effects of oligonucleotide length and locked nucleic acid content on splice-switching oligonucleotide activity
T. Shimo, K. Tachibana, K. Saito, T. Yoshida, E. Tomita, R. Waki, T. Yamamoto, T. Doi, T. Inoue, J. Kawakami, and S. Obika
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 332 - 333 2014年11月
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A novel ribozyme core to perform self-cleavage reaction
S. Tanaka, N. Sakimoto, M. Kameno, N. Sasaki, and J. Kawakami
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 340 - 341 2014年11月
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担当区分:筆頭著者
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Design and evaluation of locked nucleic acid-based splice-switching oligonucleotides in vitro 査読あり
T. Shimo, K. Tachibana, K. Saito, T. Yoshida, E. Tomita, R. Waki, T. Yamamoto, T. Doi, T. Inoue, J. Kawakami and S. Obika
Nucleic Acids Res. 42 8174 - 8187 2014年6月
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Thermodynamic Features Provided by an ENA Introduction to Nucleic Acid Drugs
E. Tomita, M. Sumimoto, J. Kawakami
Proc. 41st Intl. Symp. Nucleic Acid Chemistry 188 - 189 2014年
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Positional Effect of 2',4'-BNA/LNA Introduction into DNA/RNA Duplexes on Thermodynamic Parameters
S. Tahara, E. Tomita, H. Nagai, S. Obika, and J. Kawakami
Proc. 40th Intl. Symp. Nucleic Acid Chemistry 190 - 191 2013年11月
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担当区分:筆頭著者
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Specific recognition of cytosine by hypoxanthine in pyrrolidinyl peptide nucleic acid 査読あり
C. Vilaivan, W. Srinarang, N. Yotapan, W. Mansawat, C. Boonlua, J. Kawakami, Y. Yamaguchi, Y. Tanaka, and T. Vilaivan
Org. Biomol. Chem. 11 2310 - 2317 2013年
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Construction of a trans-acting system of a L-histidine dependent ribozyme based on the secondary structure
S. Tanaka, Y. Yamaguchi, R. Takamine, and J. Kawakami
Proc. 39th Intl. Symp. Nucleic Acid Chemistry 234 - 235 2012年11月
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担当区分:筆頭著者
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Structure-function relationship of a histidine dependent ribozyme
Y. Yamaguchi, Y. Ishibashi, S. Tanaka, A. Tanigawa, I. Matsunaga, and J. Kawakami
Proc. 38th Intl. Symp. Nucleic Acids Chemistry 194 - 195 2011年11月
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担当区分:筆頭著者
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Triplet analysis that identifies unpaired regions of functional RNAs(共著) 査読あり
J. Kawakami, Y. Yamaguchi, and N. Sugimoto
Journal of Nucleic Acids 2011 doi:10.4061/2011/471843 2011年10月
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Pyrrolidinyl peptide nucleic acid with α/β-peptide backbone - A conformationally constrained PNA with unusual hybridization properties
C. Vilaivan, C. Srisuwannaket, C. Ananthanawat, C. Suparpprom, J. Kawakami, Y. Yamaguchi, Y. Tanaka, T. Vilaivan
Artificial DNA: PNA & XNA 2 11 2011年
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Comparative thermodynamic analysis of RNA-protein interaction on surface and in solution(共著)
Y. Tanaka, K. Ishidate, K. Kishimoto, N. Sugimoto, and J. Kawakami
Proc. 37th Intl. Symp. Nucleic Acids Chemistry 276 - 277 2010年11月
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担当区分:筆頭著者
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Accurate curve fitting procedure for UV melting analysis of highly thermostable RNA hairpins(共著)
J. Kawakami, Y. Tanaka, and K. Kishimoto
Nucleic Acids Symp. Ser. 53 227 - 228 2009年9月
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Recognition of a flipped base in a hairpinloop DNA by a small peptide (共著) 査読あり
J. Kawakami, S. Okabe, Y. Tanabe, and N. Sugimoto
Nucleoside, Nucleotides and Nucleic Acids 27 292 - 308 2008年2月
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Functional role of the cofactor on activation process of L-histidine dependent ribozyme(共著)
J. Kawakami, C. Maiya, and N. Sugimoto
Nucleic Acids Symp. Ser. 50 241 - 242 2006年11月
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担当区分:筆頭著者
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A novel stable RNA pentaloop that interacts specifically with a motif peptide of lambda-N protein(共著) 査読あり
J. Kawakami, H. Tokitoh, Y. Tanabe, and N. Sugimoto
Nucleoside, Nucleotides and Nucleic Acids 25 397 - 416 2006年4月
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Specific recognition of the basic N peptide by an RNA(共著)
J. Kawakami, H. Tokitoh, Y. Tanabe, and N. Sugimoto
Nucleic Acids Symposium Series 49 353 - 354 2005年9月
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担当区分:筆頭著者
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Thermodynamic analysis of duplex formation of the heterochiral DNA with L-deoxyadenosine(共著) 査読あり
J. Kawakami, K. Tsujita, and N. Sugimoto
Analytical Sciences 21 ( 2 ) 77 - 82 2005年2月
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担当区分:筆頭著者