Papers - MIYOSHI Daisuke
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Significant structural change in human c-Myc promoter G-quadruplex Reviewed International coauthorship
Nikita Kundu, Taniya Sharma, Sarvpreet Kaur, Mamta Singh, Vinit Kumar, Uttam Sharma, Aklank Jain, Jadala Shankaraswamy, Daisuke Miyoshi, Sarika Saxena
RSC Adv. 12 7594 - 7604 2022.4
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Intramolecular G-quadruplex-hairpin loop structure competition of a GC-rich exon region in the TMPRSS2 gene Reviewed
Chem. Commun. 58 48 - 51 2022.1
Authorship：Corresponding author
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Wataru Sugimoto, Natsuki Kinoshita, Minori Nakata, Tatsuya Ohyama, Hisae Tateishi-Karimata, Takahito Nishikata, Naoki Sugimoto, Daisuke Miyoshi, Keiko Kawauchi
Chemical Communications 58 ( 1 ) 48 - 51 2022
Publisher：Royal Society of Chemistry (RSC)
We identified cytosine-rich regions adjacent to guanine-rich regions in the TMPRSS2 gene, which showed structural competition between a G-quadruplex and a hairpin loop. Furthermore, this competition significantly affected transcription efficiency.
DOI： 10.1039/d1cc05523b
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核酸の四重らせん構造を狙った治療薬の展望 Invited
37 ( 4 ) 52 - 55 2022
Authorship：Corresponding author
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rDNAおよびrRNAが形成する四重らせん構造による核小体機能の制御 Invited
川内敬子、取井猛流、月生雅也、木下菜月、三好大輔
月刊細胞 54 ( 12 ) 36 - 39 2022
Authorship：Corresponding author
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Detection of Intracellular Reactive Oxidative Species Using the Fluorescent Probe Hydroxyphenyl Fluorescein Invited Reviewed
Wataru Sugimoto, Daisuke Miyoshi, Keiko Kawauchi
Methods in Molecular Biology 2274 207 - 215 2021.5
Publisher：Humana Press Inc.
Various fluorescent probes for the detection of intracellular reactive oxidative species (ROS) have been developed because ROS levels are closely associated with cellular states. Here, we describe a method for detection of intracellular ROS in living cells using the fluorescent probe, hydroxyphenyl fluorescein (HPF), which detects hydroxyl radicals and peroxynitrite. NIH3T3 cells and p53 knockout (p53−/−) mouse embryonic fibroblasts (MEFs) were transformed by expressing oncogenic RAS using a retrovirus system. The cells were treated with HPF at 37 °C for 30 min, and subsequently, images were acquired using a confocal fluorescence microscope at an excitation wavelength of 488 nm after washing with PBS.
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Molecularly targeted photodynamic therapy for cancer mRNAs Invited Reviewed
杉本渉, 橋本佳樹, 川内敬子, 三好大輔
Bio Clinica 36 ( 5 ) 65 - 67 2021.5
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核小体ストレスにより形成するrDNA G-quadruplex集合体とその機能
杉本 渉, 桑本 慎, 建石 寿枝, 岩根 敦子, 高橋 宏隆, 三好 大輔, 川内 敬子
日本薬学会年会要旨集 141年会 29V05 - am10S 2021.3
Publisher：(公社)日本薬学会
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Combined Effects of Methylated Cytosine and Molecular Crowding on the Thermodynamic Stability of DNA Duplexes Invited Reviewed
Combined Effects of Methylated Cytosine, Molecular Crowding, on, the Thermodynamic Stability of, DNA Duplexes
Int. J. Mol. Sci. 22 ( 2 ) 947 2021.1
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Photosensitizers Based on G-Quadruplex Ligand for Cancer Photodynamic Therapy Invited International journal
Keiko Kawauchi, Ryoto Urano, Natsuki Kinoshita, Shin Kuwamoto, Takeru Torii, Yoshiki Hashimoto, Shinya Taniguchi, Mitsuki Tsuruta, Daisuke Miyoshi
Genes 11 ( 11 ) 1340 2020.11
Joint Work
G-quadruplex (G4) is the non-canonical secondary structure of DNA and RNA formed by guanine-rich sequences. G4-forming sequences are abundantly located in telomeric regions and in the promoter and untranslated regions (UTR) of cancer-related genes, such as RAS and MYC. Extensive research has suggested that G4 is a potential molecular target for cancer therapy. Here, we reviewed G4 ligands as photosensitizers for cancer photodynamic therapy (PDT), which is a minimally invasive therapeutic approach. The photosensitizers, such as porphyrins, were found to be highly toxic against cancer cells via the generation of reactive oxidative species (ROS) upon photo-irradiation. Several porphyrin derivatives and analogs, such as phthalocyanines, which can generate ROS upon photo-irradiation, have been reported to act as G4 ligands. Therefore, they have been implicated as promising photosensitizers that can selectively break down cancer-related DNA and RNA forming G4. In this review, we majorly focused on the potential application of G4 ligands as photosensitizers, which would provide a novel strategy for PDT, especially molecularly targeted PDT (mtPDT).
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TakahiroYamauchi, Takafumi Kubodera, Daisuke Miyoshi, Naoki Sugimoto, Shuji Hirohata
Journal of Bioscience and Bioengineering 2020.10
Joint Work
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Photodynamic therapy targeting RAS mRNA
Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi
Medical Science Digest 46 ( 9 ) 26 - 27 2020.7
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Novel Strategy of Photodynamic Therapy Targeting RAS mRNA with G-Quadruplex Ligands for Cancer Treatment Invited Reviewed
Takeru Torii, Wataru Sugimoto, Keiko Kawauchi, Daisuke Miyoshi
Journal of Data Mining in Genomics & Proteomics 2020.6
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RNA Phase Separation-Mediated Direction of Molecular Trafficking Under Conditions of Molecular Crowding Invited Reviewed
Kazuki Kohata, Daisuke Miyoshi
Biophys. Rev. 12 669 - 676 2020.5
Joint Work
Living cells are highly crowded with large and small biomolecules. The total concentration of biomolecules can reach 400 mg/ml, and 40% of the cell volume is occupied by biomolecules. Droplet formation in cells via liquid-liquid phase separation may play a role in controlling biochemical reactions in this complex molecular environment. Liquid-liquid phase separation generally involves nucleic acids and proteins as anionic and cationic components, respectively. Significant characteristics of droplets, which make them different from protein aggregation or fibril formation, are reversibility of formation and responsiveness to the molecular environment. In this review, we quantitatively describe the molecular environment inside cells and droplets that participate in controlling central dogma reactions. Finally, we discuss the importance of droplets under conditions of molecular crowding within living cells.
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Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA Reviewed
H. Tateishi-Karimata, D. Banerjee, T. Ohyama, S. Matsumoto, D. Miyoshi, S. Nakano, and N. Sugimoto
Biochemical and Biophysical Research Communications 525 ( 1 ) 177 - 183 2020.4
Joint Work
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Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA. Reviewed International journal
Hisae Tateishi-Karimata, Dipanwita Banerjee, Tatsuya Ohyama, Saki Matsumoto, Daisuke Miyoshi, Shu-Ich Nakano, Naoki Sugimoto
Biochemical and biophysical research communications 2020.2
Telomeric G-quadruplex topology has the ability to regulate telomerase activity, which counteracts the shortening of telomere with successive cell divisions, thereby causing genomic longevity. However, the detailed mechanism of G-quadruplexes topologies formed by telomeric sequences requires further investigation. In this study, we quantitatively investigated the effect of cosolutes, particularly the varying number of hydroxyl groups, on the structural transition between hybrid type and parallel G-quadruplexes formed by telomeric DNA sequences. Cosolutes with one or no hydroxyl groups in the vicinal position more efficiently induced the transition to parallel G-quadruplex from hybrid G-quadruplex than those with more hydroxyl groups. We also examined the effect of cosolute structures on the hydration of G-quadruplex formation; the results indicated that cosolutes with fewer hydroxyl groups lead to the release of greater amount of water during G-quadruplex formation. Molecular dynamics results showed that the parallel G-quadruplex was more dehydrated than the hybrid type G-quadruplex. Generally, a dehydrated structure is favored under crowding condition. Thus, depending on the surrounding cosolutes, the G-quadruplex topology can be controlled by the G-quadruplex hydration state.
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Shikhar Tyagi, Sarika Saxena, Nikita Kundu, Taniya Sharma, Amlan Chakraborty, Sarvpreet Kaur, Daisuke Miyoshi, Jadala Shankaraswamy
RSC Advances 9 40255 - 40262 2019.12
Joint Work
We described a novel synthetic peptide in which a glutamine residue binds through hydrogen bonding to a guanine-base and a trytophan residue intercalates with K+ resulting in stabilization of a human telomeric G-quadruplex with high selectivity over its complementary c-rich strand and a double-stranded DNA and its complementary C-rich strand. This peptide offers great potential for cancer treatment by inhibiting the telomere extension by telomerase.
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The RAS mRNA G-quadruplex: making undruggable cancer targets druggable
Keiko Kawauchi, Wataru Sugimoto, Daisuke Miyoshi
The Atlas of Science 2019.10
Joint Work
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細胞の分子夾雑環境における核酸構造と核酸構造リガンドの挙動 ～細胞内でも望みの機能を発現する分子の合理設計に向けて～
三好大輔
生命化学研究レター 58 ( 1 ) 17 - 21 2019.4
Single Work