論文 - 甲元 一也
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Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size 査読あり 国際誌
Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
BIOCONJUGATE CHEMISTRY 26 ( 8 ) 1775 - 1781 2015年8月
出版者・発行元：AMER CHEMICAL SOC
The design and synthesis of materials capable of activating the immune system in a safe manner is of great interest in immunology and related fields. Lactobacilli activate the innate immune system of a host when acting as probiotics. Here, we constructed lactobacilli-mimicking materials in which polysaccharide peptidoglycan complexes (PS PGs) derived from lactobacilli were covalently conjugated to the surfaces of polymeric microparticles with a wide variety of sizes, ranging from 200 nm to 3 mu m. The artificial lactobacilli successfully stimulated macrophages without cytotoxicity. Importantly, we found that the size of artificial lactobacilli strongly influenced their immunostimulating activities, and that artificial lactobacilli of 1 mu m exhibited 10-fold higher activity than natural lactobacilli. One major advantage of the artificial lactobacilli is facile control of size, which cannot be changed in natural lactobacilli. These findings provide new insights into the design of materials for immunology as well as the molecular biology of lactobacillus.
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Mechanistic study of unusual enzyme activation caused by addition of inert betaine-type metabolite and the analogs
Yuichi Nakagawa, Kazuya Koumoto
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 249 2015年3月
出版者・発行元：AMER CHEMICAL SOC
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Synthesis and lmmunestimulating Activity of Lactobacilli-Originated Polysaccharide-Polymeric Microparticle Conjugates 査読あり 国際誌
Koji Nagahama, Takayuki Kumano, Tsubasa Nakata, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
LANGMUIR 31 ( 4 ) 1489 - 1495 2015年2月
出版者・発行元：AMER CHEMICAL SOC
The design and synthesis of biomaterials capable of activating the immune system are of interest in immunology-related fields because of their ability to tune up the immune defenses of the host. Lactobacilli are a major constituent of normal human indigenous flora, and some specific strains are known to activate the immune system of the host as probiotics. In this study, we first fabricated novel biohybrid materials in which lactobacilli (L. casei strain Shirota, LcS)-originated polysaccharide-peptidoglycan complexes (PS-PGs) are conjugated with polymeric microparticles (MPs). PS-PGs conjugated onto polymeric MPs surfaces bound its specific antibody, suggesting that PS-PGs kept their original molecular recognition ability. The PS-PGs-based hybrid MPs with an appropriate density of conjugated PS-PGs effectively induced high levels of IL-12 production from macrophages without cytotoxicity. These results suggest that LcS-originated PS-PGs could be available bio-originated materials for developing novel biomaterials capable of activating the immune system in a safe manner.
DOI： 10.1021/la5041757
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Cryoprotective ability of betaine-type metabolite analogs during freezing denaturation of enzymes 査読あり
Yuichi Nakagawa, Masahiro Sota, Kazuya Koumoto
Biotechnology Letters 37 1607 - 1613 2015年
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担当区分：筆頭著者
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Synthesis and Immunestimulating Activity of Lactobacilli-Originated Polysaccharide-Polymeric Microparticle Conjugates 査読あり
Koji Nagahama, Takayuki Kumano, Tsubasa Nakata, Hirokazu Tsuji, Kaoru Moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
Langmuir 31 1489 - 1495 2015年
共著
担当区分：筆頭著者
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Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size 査読あり
Koji Nagahama, Takayuki Kumano, Yuichi Nakagawa, Naho Oyama, Hirokazu Tsuji, Kaoru moriyama, Kan Shida, Koji Nomoto, Katsuyoshi Chiba, Kazuya Koumoto, Jun Matsui
Bioconjugate Chemistry 26 ( 8 ) 1775 - 1781 2015年
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担当区分：筆頭著者
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Significance of anionic functional group in betaine-type metabolite analogs on the facilitation of enzyme reactions 国際誌
Yuichi Nakagawa, Kotomi Takagi, Ryutaro Genjima, Kazuya Koumoto
Bioprocess and Biosystems Engineering 38 ( 9 ) 1811 - 1817 2015年
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Enhanced Chromogenic Sensitivity of Horseradish Peroxidase-catalyzed Oxidative Reactions in the Presence of Betaine-type Metabolite Analogs 査読あり
Kotomi Takagi, Yasuhiro Kashima, Satoshi Fujii, Kazuya Koumoto
Bulletin of the Chemical Society of Japan 88 ( 8 ) 1074 - 1082 2015年
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化学修飾技術を利用した乳酸菌模倣粒子による免疫賦活 招待あり
甲元一也、長濱宏治、松井淳
バイオインダストリー 32 ( 9 ) 42 - 47 2015年
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担当区分：筆頭著者
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Mechanistic study on the facilitation of enzymatic hydrolysis by alpha-glucosidase in the presence of betaine-type metabolite analogs 査読あり
Yuichi Nakagawa, Shiro Sehata, Satoshi Fujii, Hiroaki Yamamoto, Akihiko Tsuda, Kazuya Koumoto
TETRAHEDRON 70 ( 35 ) 5895 - 5903 2014年9月
出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD
Recently we reported that betaine-type metabolite analogs structure-dependently facilitate enzymatic hydrolysis reaction for alpha-glucosidases, beta-glucosidases, and alkaliphosphatases. To understand the facilitation mechanism for enzymes, in this study we expanded the analog library and measured the properties of analog solutions. The structural investigation on alpha-glucosidase-mediated hydrolysis reaction indicated that suitable structures to facilitate the enzyme reaction efficiently should have the ammonium cation in the betaine structure possess triplicate aliphatic chains from Cl to C7 without any polar functional groups. Analyses of the solution properties revealed that such analogs possess a large hydration layer with low water density. Such a specific hydration environment is generated by the characteristic structure of the betaine-type metabolite analogs. The characteristic hydration indirectly regulates enzyme activity and stability. These findings not only increase our understanding enzyme activation by betaine-type metabolite analogs, but also will contribute to the molecular design of enzyme regulators. (C) 2014 Elsevier Ltd. All rights reserved.
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Mechanistic study on the facilitation of enzymatic hydrolysis by α-glucosidase in the presence of betaine-type metabolite analogs 査読あり
Yuichi Nakagawa, Shiro Sehata, Satoshi Fujii, Hiroaki Yamamoto, Akihiko Tsuda, Kazuya Koumoto
Tetrahedron 70 5895 - 5903 2014年
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担当区分：筆頭著者
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Cellular zwitterionic metabolite analogs simultaneously enhance reaction rate, thermostability, salt tolerance, and substrate specificity of alpha-glucosidase 査読あり
Eisuke Deguchi, Kazuya Koumoto
Bioorganic and Medicinal Chemistry 19 3128 - 3134 2011年4月
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担当区分：筆頭著者
天然の代謝産物を模倣し、化学的に合成した代謝産物アナログがα−グルコシダーゼ、β−グルコシダーゼ、アルカリフォスファターゼといった加水分解酵素の活性（反応速度、耐熱性、耐塩性、基質選択性等）を溶液に共存させるだけで向上させることを明らかとした。
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Reversible stability switching of a hairpin DNA via a photo-responsive linker unit 招待あり 査読あり
Li Wu, Kazuya Koumoto, Naoki Sugimoto
Chemical Communications 1915 - 1917 2009年4月
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ヘアピンDNAのループ部位に導入し、光異性化によってヘアピンDNA全体の安定性を制御できる新規光応答性ユニットの開発に成功した。このユニットをヘアピン部位に導入したDNAは紫外光、可視光の照射に伴って安定性が可逆的に制御することができた。
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Structural effect of synthetic zwitterionic cosolutes on the stability of DNA duplexes 査読あり
甲元一也
Tetrahedron ( 64 ) 168 - 174 2008年11月
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担当区分：筆頭著者
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Artificial G-wire Switch with 2,2’-Bipyridine Units Responsive to Divalent Metal Ions
甲元一也
J. Am. Chem. Soc ( 129 ) 5919 - 5925 2007年11月
単著
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５）天然高分子 核酸 エネルギーデータベースに基づく核酸医薬の設計
甲元一也
絵で見てわかるナノDDS 遺伝子医学MOOK別冊（メディカルドゥ） 63 - 69 2007年11月
単著
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Conformational switch of a functional nanowire based on the DNA G-quadruplex
甲元一也
Nucleic Acids Symp. Ser. ( 51 ) 251 - 252 2007年11月
単著
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Delivery of antisense oligonucleotides to nuclear telomere RNA by use of a complex between polysaccharide and polynucleotide
甲元一也
Bull. Chem. Soc. Jpn ( 80 ) 1091 - 1098 2007年11月
単著
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Schizophyllan, beta-1,3-glucan,-oligoamine conjugates as non-viral vector 査読あり
Takeshi Nagasaki, Atsushi Uno, Kazuya Koumoto, Kazuo Sakurai, Seiji Shinkai
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 234 2007年8月
出版者・発行元：AMER CHEMICAL SOC
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Galactose-PEG Dual Conjugation of b-(1→3)-D-glucan schizophyllan for antisense oligonucleotide delivery to enhance cellular uptake
甲元一也
Biomaterials, ( 27 ) 1626 - 1635 2006年11月
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