Details of Database of Faculty members and Researchers

アミロイドβペプチドとグアニンリッチDNAを用いた新規ナノ構造体の作製 Reviewed

下岡正幸, 岡田亜梨沙, 柳原太志, 臼井健二

日本化学会講演予稿集 95th ( 3 ) 2015

第46回若手ペプチド夏の勉強会開催 Reviewed

臼井健二

Peptide Newsletter Japan 94 2014.10

A PNA Peptide for the Control of Site-Specific Silica Precipitation on DNA Reviewed

USUI Kenji, NAGAI Kazuma, NISHIYAMA Hiroto, TSURUOKA Takaaki, FUJII Satoshi

2012 375 - 376 2013.3

Construction of Artificial Peptides to Control DNA G-quadruplex Structures Depending on Protease Activity for Regulation of Protein Expression Reviewed

OKADA Arisa, USUI Kenji, TANIGUCHI Mai, KOBAYASHI Keita, SUGIMOTO Naoki

2012 285 - 286 2013.3

Designed peptide with peptide nucleic acids for addressable precipitation of silica on DNA

Kenji Usui, Kazuma Nagai, Hiroto Nishiyama, Tamaki Endoh, Takaaki Tsuruoka, Satoshi Fujii

PROTEIN SCIENCE 21 122 - 123 2012.8

Publishing type：Research paper, summary (international conference) Publisher：WILEY-BLACKWELL

Designed peptides with nucleic acid bases for control of DNA G-quadruplex structures depending on protease activity

Arisa Okada, Mai Taniguchi, Keita Kobayashi, Kenji Usui, Naoki Sugimoto

PROTEIN SCIENCE 21 74 - 74 2012.8

Publishing type：Research paper, summary (international conference) Publisher：WILEY-BLACKWELL

“オミクス”研究に向けた設計ペプチドアレイの構築 Reviewed

臼井健二、富崎欣也、三原久和

分析化学 61 523 - 534 2012.6

Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：日本分析化学会

A NOVEL NITRIC OXIDE SENSOR USING FLUORESCENT PEPTIDES ATTACHED TO IRON COMPLEXES

H. Miyazaki, K. Usui, S. Fujii

BIOPOLYMERS 96 ( 4 ) 510 - 510 2011

Publishing type：Research paper, summary (international conference) Publisher：WILEY-BLACKWELL

Fluorescent and luminescent fusion proteins for detection of amyloid beta peptide localization and aggregation

K. Usui, M. Mie, T. Andou, N. Sugimoto, H. Mihara, E. Kobatake

JOURNAL OF PEPTIDE SCIENCE 16 175 - 175 2010.9

Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS LTD

第１０章デザインペプチドを用いたプロテインアレイと細胞アレイ

臼井健二, 富崎欣也, 三原久和

化学フロンティア２２生命現象を理解する分子ツール、(化学同人) 79 - 87 2010.9

Protein-protein interactions and selection: array-based techniques for screening disease-associated biomarkers in predictive/early diagnosis Reviewed

K.-Y. Tomizaki, K. Usui, H. Mihara

FEBS Journal 277 1996 - 2005 2010.5

Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：the Federation of European Biochemical Societies

Proteins: Array-Based Techniques Reviewed

K.-Y. Tomizaki, K. Usui and H. Mihara

Wiley Encyclopedia of Chemical Biology 4 144 - 158 2009.1

Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：Wiley

細胞内外でのアミロイドβペプチドの局在と構造変化を検出できる発光・蛍光タンパク質の構築 Reviewed

臼井健二, 三重正和, 杉本直己, 三原久和, 小畠英理

日本化学会バイオテクノロジー部会シンポジウム講演要旨集 12th 2009

種々の細胞系を用いた新規αヘリックスペプチドの細胞導入活性スクリーニング Reviewed

菊池卓哉, 臼井健二, 高橋剛, 三原久和

日本化学会バイオテクノロジー部会シンポジウム講演要旨集 12th 2009

ペプチドライブラリを用いるタンパク質相互作用検出チップ Reviewed

富崎欣也・臼井健二・三原久和

Electrochemistry 75 ( 12 ) 981 - 985 2007

Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：電気化学会

A peptide array with a functional linker toward a cell assay chip format

T. Kakiyama, K. Usui, K. Tomizaki, H. Mihara

JOURNAL OF PEPTIDE SCIENCE 12 236 - 236 2006

Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS LTD

Detection of Interactions Between the Peptides Containing Nucleobase Amino Acids and Proteins using Anomalous Reflection of Gold Reviewed

渡辺晋也, 臼井健二, 富崎欣也, 梶川浩太郎, 三原久和

日本化学会講演予稿集 86th ( 2 ) 2006

設計ペプチドを用いるアレイフォーマット開発とタンパク機能解析 Reviewed

臼井健二, 富崎欣也, 渡辺晋也, 三原久和, 梶川浩太郎

高分子学会予稿集(CD-ROM) 55 ( 2 Disk1 ) 2006

Construction of a screening system for peptide ligands controlling calcineurin activity using designed α-helical peptide arrays Reviewed

臼井健二, 富崎欣也, 三原久和

日本化学会講演予稿集 86th ( 2 ) 2006

Construction of a PNA-DNA Hybrid Array Potential for a Novel Addressable Chip Reviewed

SANO Shusuke, USUI Kenji, TOMIZAKI Kin-ya, MIHARA Hisakazu

2004 387 - 388 2005.3

Peptide Microarrays Using Designed Libraries for Protein Detection Reviewed

MIHARA Hisakazu, USUI Kenji, OJIMA Tetsunori, SUZUKI Masato, WATANABE Sin-ya, TOMIZAKI Kin-ya, NOKIHARA Kiyoshi

2004 113 - 114 2005.3

Studies on Fluorescent Dyes and Surface Chemistry focusing on Practical Peptide Array Preparation

NOKIHARA Kiyoshi, USUI Kenji, YONEMURA Koichi, OHYAMA Takafumi, OKA Yasuo, TOMIZAKI Kin‐ya, MIHARA Hisakazu

Pept Sci 2004 145 - 148 2005.3

A protein detection system with designed peptides using anomalous reflection of gold

S Watanabe, K Usui, K Tomizaki, H Mihara, M Watanabe, K Kajikawa

BIOPOLYMERS 80 ( 4 ) 599 - 599 2005

Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS INC

Designed peptide microarrays for protein detection and characterization

H Mihara, K Usui, K Tomizaki, K Nokihara

BIOPOLYMERS 80 ( 4 ) 493 - 493 2005

Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS INC

alpha-helical peptide microarrays using a dry peptide array method

K Usui, K Tomizaki, K Nokihara, H Mihara

BIOPOLYMERS 80 ( 4 ) 599 - 599 2005

Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS INC

Protein-Detecting Microarrays: Current Accomplishments and Requirements

K.-Y. Tomizaki, K. Usui and H. Mihara

ChemBioChem 6 782 - 799 2005

Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：WILEY-VCH

Construction of Protein-Detection System using anomalous reflection of gold Reviewed

渡辺晋也, 臼井健二, 富崎欣也, 渡辺光昭, 梶川浩太郎, 三原久和

日本化学会講演予稿集 85th ( 2 ) 2005

OPTIMIZATION OF PEPTIDE ARRAYS ON A NOVEL PROTEIN CHIP FOR A PRACTICAL PROTEIN CHARACTERIZATION SYSTEM

K. Nokihara, T. Ohyama, K. Yonemura, N. Sugioka, K. Usui, T. Ojima, H. Mihara

JOURNAL OF PEPTIDE SCIENCE 10 116 - 116 2004

Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS LTD

PROTEIN-DETECTION MICROARRAYS USING STRUCTURE-BASED DESIGNED PEPTIDE LIBRARIES

K. Usui, T. Ojima, M. Suzuki, S. Watanabe, K. Tomizaki, K. Nokihara, H. Mihara

JOURNAL OF PEPTIDE SCIENCE 10 158 - 158 2004

Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS LTD

Peptide microarrays using structure-based peptide libraries for protein chips

Kenji Usui, Mizuki Takahashi, Tetsunori Ojima, Masato Suzuki, Kiyoshi Nokihara, Eiichi Tamiya, Hisakazu Mihara

Peptide Revolution: Genomics, Proteomics & Therapeutics 258 - 259 2004

Publisher：AMER CHEMICAL SOC

ペプチドアレイを用いたプロテインチップの実用化を目指した研究 Reviewed

軒原清史・大山貴史・臼井健二・米村耕一・富崎欣也・三原久和

高分子論文集 61 523 - 532 2004

Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：高分子学会

Development of Peptide-Chips Focusing on a High Throughput Protein-Detection System Reviewed

K. Nokihara, T. Ohyama, K. Usui, K. Yoneyama, M. Takahashi and H. Mihara

Innovation and Perspectives in Solid Phase Synthesis & Combinatorial Libraries 83 - 88 2004

Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：Mayflower Worldwide Limited

Construction of Protein-Detection System with Peptides Containing Nucleobase Amino Acids (NBAs) Reviewed

渡辺晋也, 臼井健二, 富崎欣也, 三原久和

日本化学会講演予稿集 84th ( 2 ) 2004

Construction of a Phosphopeptide Array for Kinase-related Protein Detection Reviewed

鈴木正人, 臼井健二, 富崎欣也, 三原久和

日本化学会講演予稿集 84th ( 2 ) 2004

Development of a practical protein-chip using designed synthetic peptide-arrays Reviewed

K Nokihara, T Ohyama, K Usui, K Yonemura, K Tomizaki, H Mihara

KOBUNSHI RONBUNSHU 61 ( 10 ) 523 - 532 2004

Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：SOC POLYMER SCIENCE JAPAN

Novel high-throughput technologies, which can replace conventional electrophoresis and mass spectroscopic analyses, are in great demand for understanding the structure-function relationships of proteins. For a practical protein-detection system, arrays with immobilized designed synthetic peptides have been constructed. Peptides were labeled with fluorescent dyes in order to achieve high sensitivity. Two different types of prototype-arrayer have been constructed: one has a micro-dispensing system and the other a spot-printing system. Combinatorial peptide libraries, which consisted of beta-loop, beta-strand and alpha-helical peptides, were constructed by improved highly efficient solid-phase synthesis. Labeled peptides were covalently immobilized on solid supports such as precision glass plates. Various proteins were characterized with these peptide-arrays using a fluorescent scanner to give the "protein fingerprint" which is characteristic of the individual proteins. The results of the present prototype system demonstrate the practicality of protein-chips as a new generation of biochips.

プロテインチップ (特集1 バイオと化学産業の接点) -- (第1章医療分野におけるバイオ) Reviewed

ペプチドマイクロアレイ～ペプチドライブラリを用いた新規プロテインチップの創製～

臼井健二,三原久和

Bioベンチャー 3 99 - 102 2003.8

Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：羊土社

臼井健二, 三原久和

化学装置 45 ( 4 ) 36 - 38 2003.4

Publisher：工業調査会