Review Papers (Misc) - USUI Kenji
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Protein-Detecting Microarrays: Current Accomplishments and Requirements
K.-Y. Tomizaki, K. Usui and H. Mihara
ChemBioChem 6 782 - 799 2005
Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：WILEY-VCH
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Construction of Protein-Detection System using anomalous reflection of gold Reviewed
渡辺晋也, 臼井健二, 富崎欣也, 渡辺光昭, 梶川浩太郎, 三原久和
日本化学会講演予稿集 85th ( 2 ) 2005
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OPTIMIZATION OF PEPTIDE ARRAYS ON A NOVEL PROTEIN CHIP FOR A PRACTICAL PROTEIN CHARACTERIZATION SYSTEM
K. Nokihara, T. Ohyama, K. Yonemura, N. Sugioka, K. Usui, T. Ojima, H. Mihara
JOURNAL OF PEPTIDE SCIENCE 10 116 - 116 2004
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS LTD
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PROTEIN-DETECTION MICROARRAYS USING STRUCTURE-BASED DESIGNED PEPTIDE LIBRARIES
K. Usui, T. Ojima, M. Suzuki, S. Watanabe, K. Tomizaki, K. Nokihara, H. Mihara
JOURNAL OF PEPTIDE SCIENCE 10 158 - 158 2004
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS LTD
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Peptide microarrays using structure-based peptide libraries for protein chips
Kenji Usui, Mizuki Takahashi, Tetsunori Ojima, Masato Suzuki, Kiyoshi Nokihara, Eiichi Tamiya, Hisakazu Mihara
Peptide Revolution: Genomics, Proteomics & Therapeutics 258 - 259 2004
Publisher：AMER CHEMICAL SOC
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ペプチドアレイを用いたプロテインチップの実用化を目指した研究 Reviewed
軒原清史・大山貴史・臼井健二・米村耕一・富崎欣也・三原久和
高分子論文集 61 523 - 532 2004
Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：高分子学会
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Development of Peptide-Chips Focusing on a High Throughput Protein-Detection System Reviewed
K. Nokihara, T. Ohyama, K. Usui, K. Yoneyama, M. Takahashi and H. Mihara
Innovation and Perspectives in Solid Phase Synthesis & Combinatorial Libraries 83 - 88 2004
Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution) Publisher：Mayflower Worldwide Limited
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Construction of Protein-Detection System with Peptides Containing Nucleobase Amino Acids (NBAs) Reviewed
渡辺晋也, 臼井健二, 富崎欣也, 三原久和
日本化学会講演予稿集 84th ( 2 ) 2004
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Construction of a Phosphopeptide Array for Kinase-related Protein Detection Reviewed
鈴木正人, 臼井健二, 富崎欣也, 三原久和
日本化学会講演予稿集 84th ( 2 ) 2004
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Development of a practical protein-chip using designed synthetic peptide-arrays Reviewed
K Nokihara, T Ohyama, K Usui, K Yonemura, K Tomizaki, H Mihara
KOBUNSHI RONBUNSHU 61 ( 10 ) 523 - 532 2004
Publishing type：Article, review, commentary, editorial, etc. (scientific journal) Publisher：SOC POLYMER SCIENCE JAPAN
Novel high-throughput technologies, which can replace conventional electrophoresis and mass spectroscopic analyses, are in great demand for understanding the structure-function relationships of proteins. For a practical protein-detection system, arrays with immobilized designed synthetic peptides have been constructed. Peptides were labeled with fluorescent dyes in order to achieve high sensitivity. Two different types of prototype-arrayer have been constructed: one has a micro-dispensing system and the other a spot-printing system. Combinatorial peptide libraries, which consisted of beta-loop, beta-strand and alpha-helical peptides, were constructed by improved highly efficient solid-phase synthesis. Labeled peptides were covalently immobilized on solid supports such as precision glass plates. Various proteins were characterized with these peptide-arrays using a fluorescent scanner to give the "protein fingerprint" which is characteristic of the individual proteins. The results of the present prototype system demonstrate the practicality of protein-chips as a new generation of biochips.
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ペプチドマイクロアレイ ～ペプチドライブラリを用いた新規プロテインチップの創製～
臼井健二,三原久和
Bioベンチャー 3 99 - 102 2003.8
Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media) Publisher：羊土社
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Structure-designed Peptide Microarrays for Protein-Detection Reviewed
MIHARA Hisakazu, TAKAHASHI Mizuki, USUI Kenji, OJIMA Tetsunori, NOKIHARA Kiyoshi
2002 109 - 110 2003.2
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Microarrays with alpha-Helical Peptides for Protein Detection using a FRET Technique
K. Usui, M. Takahashi, A. Ueno, K. Nokihara, H. Mihara
Peptides 2002 646 - 647 2003
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Peptide microarrays using structure-based peptide libraries for protein chips
K Usui, M Takahashi, T Ojima, M Suzuki, K Nokihara, F Tamiya, H Mihara
BIOPOLYMERS 71 ( 3 ) 403 - 403 2003
Publishing type：Research paper, summary (international conference) Publisher：JOHN WILEY & SONS INC
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Construction of Protein-Detection Microarrays with FRET Fingerprint Patterns Using an α-Helical Peptide Library. Reviewed
臼井健二, 高橋瑞稀, 軒原清史, 三原久和
生体機能関連化学シンポジウム講演要旨集 18th 2003
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Construction of a Protein-Detection System using Designed Peptides with Fluorescent Labels
K. Usui, M. Takahashi, A. Ueno, K. Nokihara, H. Mihara
Peptide Science 2001 405 - 406 2002