論文 - 臼井 健二
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DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity 査読あり
Kenji Usui, Arisa Okada, Shungo Sakashita, Masayuki Shimooka, Takaaki Tsuruoka, Shu-ichi Nakano, Daisuke Miyoshi, Tsukasa Mashima, Masato Katahira, Yoshio Hamada
Molecules 22 ( 11 ) 1991 2017年11月
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Fluorescent and Luminescent Fusion Proteins for Analyses of Amyloid Beta Peptide Aggregation 査読あり
Usui K, Mie M, Andou T, Mihara H, Kobatake E.
J Pept Sci. 23 ( 7-8 ) 659 - 665 2017年7月
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Development of Peptide Microarray to Detect the Protein in Saliva for Periodontal Disease Test 査読あり
Yuki Tominaga, Kenji Usui, Akiyoshi Hirata, Atsushi Kitagawa, Hiro-O Ito and Kiyoshi Nokihara
Peptide Science 2016 131 - 132 2017年3月
共著
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A Systematic Study on Molecular Mechanism of Pore-Forming Peptides for Discovering Antimicrobial Medicine
Y. Sekiya, H. Watanabe, K. Usui, R. Kawano
Proceedings of MicroTAS 2016 595 - 596 2016年10月
共著
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Site-Specific Control of Silica Mineralization on DNA Using a Designed Peptide 査読あり
M. Ozaki, K. Nagai, H. Nishiyama, T. Tsuruoka, S. Fujii, T. Endoh, T. Imai, K.-y. Tomizaki, K. Usui
Chem. Commun. 52 4010 - 4013 2016年3月
共著
担当区分：筆頭著者
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Development of Designed Peptides with Secondary Structure for Use in Nanobiotechnology 招待あり 査読あり
K.Usui
Peptide Science 2015 5 - 8 2016年3月
単著
担当区分：筆頭著者
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A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis. 査読あり
Usui K, Tomizaki KY, Mihara H
Methods in molecular biology (Clifton, N.J.) 1352 199 - 210 2016年
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Site-specific control of multiple mineralizations using a designed peptide and DNA 査読あり
Kenji Usui, Makoto Ozaki, Aoi Yamada, Yoshio Hamada, Takaaki Tsuruoka, Takahito Imai, Kin-ya Tomizaki
NANOSCALE 8 ( 39 ) 17081 - 17084 2016年
出版者・発行元：ROYAL SOC CHEMISTRY
We have developed a site-specific method for precipitating multiple inorganic compounds using target DNA and a designed peptide consisting of a peptide nucleic acid (PNA) sequence and an inorganic compound-precipitating sequence. This system for controlled site-specific precipitation represents a powerful tool for use in nanobiotechnology and materials science.
DOI： 10.1039/c6nr03468c
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Site-specific control of silica mineralization on DNA using a designed peptide 査読あり
Makoto Ozaki, Kazuma Nagai, Hiroto Nishiyama, Takaaki Tsuruoka, Satoshi Fujii, Tamaki Endoh, Takahito Imai, Kin-ya Tomizaki, Kenji Usui
CHEMICAL COMMUNICATIONS 52 ( 21 ) 4010 - 4013 2016年
出版者・発行元：ROYAL SOC CHEMISTRY
We developed a site-specific method for precipitating inorganic compounds using organic compounds, DNA, and designed peptides with peptide nucleic acids (PNAs). Such a system for site-specific precipitation represents a powerful tool for use in nanobiochemistry and materials chemistry.
DOI： 10.1039/c5cc07870a
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ATP-Binding Peptide-Hydrogel Composite Synthesized by Molecular Imprinting on Beads 査読あり
Ayana Takata, Kenji Usui, Jun Matsui
Molecular Imprinting 3 65 - 70 2015年12月
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A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis 査読あり
K. Usui, K.-y. Tomizaki, H. Mihara
Methods. Mol. Biol. 1352 199 - 210 2015年11月
共著
担当区分：筆頭著者
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Label and Label-Free Detection Techniques for Protein Microarrays 査読あり 国際共著
Amir Syahir, Kenji Usui, Kin-ya Tomizaki, Kotaro Kajikawa, Hisakazu Mihara
Microarrays 4 ( 4 ) 228 - 244 2015年4月
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Site-Specific Mineralization of Silica and Calcium on DNAs Using a Designed Peptide 査読あり
K. Usui, H. Nishiyama, A. Yamada, M. Ozaki, T. Tsuruoka, K.-y. Tomizaki
Peptide science 2014 325 - 326 2015年3月
共著
担当区分：筆頭著者
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Control of guanine-rich DNA secondary structures depending on the protease activity using a designed PNA peptide 査読あり
Kenji Usui, Arisa Okada, Keita Kobayashi, Naoki Sugimoto
ORGANIC & BIOMOLECULAR CHEMISTRY 13 ( 7 ) 2022 - 2025 2015年
出版者・発行元：ROYAL SOC CHEMISTRY
We constructed a regulation system for DNA secondary structure formation of G-rich sequences using a designed PNA peptide exhibiting an on-to-off switching functionality, depending on the protease activity. This study introduces the new concept of a simple and powerful system for regulating quadruplex-related important biological events.
DOI： 10.1039/c4ob02535k
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Cellular differentiation assessments by measuring the degree of cellular internalization and membrane adsorption using designed peptides 査読あり
Kenji Usui, Takuya Kikuchi, Kunio Kikuchi, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 24 ( 17 ) 4129 - 4131 2014年9月
出版者・発行元：PERGAMON-ELSEVIER SCIENCE LTD
We demonstrate examples of cellular differentiation assessments, including cellular neurite outgrowth and fat cell maturation, by measuring the degree of membrane adsorption or cellular internalization using designed peptides. Because changes in the cellular membrane and cytosol during differentiation were shown to influence membrane adsorption and cellular internalization, we could successfully evaluate the extent of differentiation simply like stain indicators. (C) 2014 Elsevier Ltd. All rights reserved.
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A Designed Small Protein For Controlling Site-Specific Mineralization Of Silica And Calcium On Dnas 査読あり
Kenji Usui, Kazuma Nagai, Hiroto Nishiyama, Aoi Yamada, Makoto Ozaki, Takaaki Tsuruoka, Kin-ya Tomizaki
PROTEIN SCIENCE 23 143 - 143 2014年7月
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Site-Specific Control of Silica Mineralization on DNA Using Designed Peptides 査読あり
K. Usui, K. Nagai, H. Nishiyama, A. yamada, T. Tsuruoka, S. Fujii, K.-y. Tomizaki
Peptide Science 2013 2415 - 2416 2014年3月
共著
担当区分：筆頭著者
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Peptides targeting G-quadruplex structures 査読あり
Kenji Usui, Arisa Okada
Chemical Biology of Nucleic Acids: Fundamentals and Clinical Applications 459 - 475 2014年1月
出版者・発行元：Springer Berlin Heidelberg
Research in recent decades has revealed that some DNA and RNA secondary structures modulate a variety of cellular events. One secondary structure, the Guanine(G)-quadruplex, also regulates various cellular events that are mostly related to serious diseases. Systems capable of controlling DNA and RNA G-quadruplex structures would therefore be useful for the modulation of various cellular events to produce biological effects. Because of their biological importance, many G-quadruplex-targeting compounds have been described. However, the next generation of targeting molecules should exhibit increased G-quadruplex sequence specificity, a higher structure-inducing or -collapsing ability, and a greater degree of functionality, including on–off switches of binding ability and cellular penetration. Peptides might be good candidates for these next-generation Gquadruplex- targeting molecules due to the following advantages: (1) their easy design and synthesis, (2) their ability to mimic protein–G–quadruplex interactions, (3) the possibility of employing artificial amino acids in addition to naturally occurring amino acids, and (4) the ability to combine G-quadruplex-binding sequences with other functional sequences. Accordingly, several peptide-based compounds, such as furan-based cyclic peptides, PNA-conjugated peptides, and small molecule-peptide conjugates, have been developed. In this chapter, we introduce all these peptide ligands and describe most of the approaches for targeting G-quadruplex structures. We then conclude that peptides are among the most promising functional ligands for G-quadruplexes to control various biological events in next-generation approaches.
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Control of Site-Specific Silica Precipitation Using PNA Peptides and DNAs 査読あり
K. Usui, H. Nishiyama, K. Nagai, T. Tsuruoka, S. Fujii, K.-y. Tomizaki
Peptides 2013 162 - 163 2013年12月
共著
担当区分：筆頭著者
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Designed Peptide Libraries for Cell Analyzing Microarrays 査読あり
H. Mihara, K. Usui, H. Tsutsumi, K. Nokihara
Peptides 2013 22 - 23 2013年12月
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