写真a

松本 咲 (マツモト サキ)

MATSUMOTO Saki

職名

特任教員

専門分野

有機化学, 生体関連化学

出身大学 【 表示 / 非表示

  • 2008年04月
    -
    2012年03月

    大阪大学   理学部   化学科   卒業

出身大学院 【 表示 / 非表示

  • 2014年04月
    -
    2017年03月

    大阪大学  理学研究科  化学専攻  博士課程  修了

  • 2012年04月
    -
    2014年03月

    大阪大学  理学研究科  化学専攻  修士課程  修了

学外略歴 【 表示 / 非表示

  • 2017年04月
    -
    2019年04月

      大日本住友製薬株式会社   リサーチディビジョン、化学研究ユニット  

所属学協会 【 表示 / 非表示

  • 2019年09月
    -
    継続中
     

    日本化学会

 

論文 【 表示 / 非表示

  • Effect of molecular crowding on the stability of RNA G-quadruplexes with various numbers of quartets and lengths of loops.

    Saki Matsumoto, Hisae Tateishi-Karimata, Shuntaro Takahashi, Tatsuya Ohyama, Naoki Sugimoto

    Biochemistry     2020年06月  [査読有り]

    共著

    G-quadruplexes are non-canonical structures formed by guanine-rich regions of not only DNA but also RNA. RNA G-quadruplexes are present across the transcriptome in various regions of messenger RNAs and non-coding RNAs and play important roles in key cellular functions. Furthermore, stable RNA G-quadruplexes control the extent of biological functions, such as mRNA translation and antigen presentation. To understand and regulate functions controlled by RNA G-quadruplexes in cellular environments, which are molecularly crowded, it is important to investigate the stability of G-quadruplexes in molecular crowding. Here, we systematically investigated the thermodynamic stability of RNA G-quadruplexes with different numbers of G-quartets and lengths of loops. The molecular crowding conditions of poly(ethylene glycol) with an average molecular weight of 200 (PEG200) were found to stabilize RNA G-quadruplexes with three and four G-quartets, while G-quadruplexes with two G-quartets did not exhibit any stabilization upon addition of PEG200. On the other hand, no difference in stabilization by PEG200 was observed among the G-quadruplexes with different loop lengths. Thermodynamic analysis of the RNA G-quadruplexes revealed more appropriate motifs for the identification of G-quadruplex forming sequences. The informatics analysis with new motifs demonstrated that the distributions of G-quadruplexes in human non-coding RNAs differed depending on the number of G-quartets. Therefore, RNA G-quadruplexes with different numbers of G-quartets may present different roles in response to environmental changes in cells.

    DOI PubMed

  • Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA.

    Hisae Tateishi-Karimata, Dipanwita Banerjee, Tatsuya Ohyama, Saki Matsumoto, Daisuke Miyoshi, Shu-Ich Nakano, Naoki Sugimoto

    Biochemical and biophysical research communications     2020年02月  [査読有り]

    共著

    Telomeric G-quadruplex topology has the ability to regulate telomerase activity, which counteracts the shortening of telomere with successive cell divisions, thereby causing genomic longevity. However, the detailed mechanism of G-quadruplexes topologies formed by telomeric sequences requires further investigation. In this study, we quantitatively investigated the effect of cosolutes, particularly the varying number of hydroxyl groups, on the structural transition between hybrid type and parallel G-quadruplexes formed by telomeric DNA sequences. Cosolutes with one or no hydroxyl groups in the vicinal position more efficiently induced the transition to parallel G-quadruplex from hybrid G-quadruplex than those with more hydroxyl groups. We also examined the effect of cosolute structures on the hydration of G-quadruplex formation; the results indicated that cosolutes with fewer hydroxyl groups lead to the release of greater amount of water during G-quadruplex formation. Molecular dynamics results showed that the parallel G-quadruplex was more dehydrated than the hybrid type G-quadruplex. Generally, a dehydrated structure is favored under crowding condition. Thus, depending on the surrounding cosolutes, the G-quadruplex topology can be controlled by the G-quadruplex hydration state.

    DOI PubMed

  • Small synthetic molecule-stabilized RNA pseudoknot as an activator for -1 ribosomal frameshifting.

    Matsumoto S, Caliskan N, Rodnina MV, Murata A, Nakatani K

    Nucleic acids research   46 ( 16 ) 8079 - 8089   2018年09月

    共著

    DOI PubMed

  • Synthetic ligand promotes gene expression by affecting GC sequence in promoter.

    Matsumoto S, Iida K, Murata A, Denawa M, Hagiwara M, Nakatani K

    Bioorganic & medicinal chemistry letters   27 ( 15 ) 3391 - 3394   2017年08月

    共著

    DOI PubMed

  • Formation of a ligand-assisted complex of two RNA hairpin loops.

    Hong C, Otabe T, Matsumoto S, Dohno C, Murata A, Hagihara M, Nakatani K

    Chemistry (Weinheim an der Bergstrasse, Germany)   20 ( 18 ) 5282 - 5287   2014年04月

    共著

    DOI PubMed

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