論文 - 向 正則
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A truncated form of a transcription factor Mamo activates vasa in Drosophila embryos. 査読あり
Shoichi Nakamura, Seiji Hira, Masato Fujiwara, Nasa Miyagata, Takuma Tsuji, Akane Kondo, Hiroshi Kimura, Yuko Shinozuka, Makoto Hayashi, Satoru Kobayashi, Masanori Mukai
Communications biology 2019年11月
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H3K36 Trimethylation-Mediated Epigenetic Regulation is Activated by Bam and Promotes Germ Cell Differentiation During Early Oogenesis in Drosophila 査読あり
Mukai M, Hira S, Nakamura K, Nakamura S, Kimura H, Sato M, Kobayashi S.
Biol Open. 2015年
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担当区分:筆頭著者
ヒストン修飾H3K36me3が生殖幹細胞の分化制御に関与することを明らかにした。
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Innexin2 gap junctions in somatic support cells are required for cyst formation and for egg chamber formation in Drosophila 査読あり
M. Mukai, H. Kato, S. Hira, K. Nakamura, H. Kita, S. Kobayashi
Mech. Dev. 128 510 - 523 2011年10月
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担当区分:筆頭著者
Germ cells require intimate associations with surrounding somatic cells during gametogenesis. During oogenesis, gap junctions mediate communication between germ cells and somatic support cells. However, the molecular mechanisms by which gap junctions regulate the developmental processes during oogenesis are poorly understood. We have identified a female sterile allele of innexin2 (inx2), which encodes a gap junction protein in Drosophila. In females bearing this inx2 allele, cyst formation and egg chamber formation are impaired. In wild-type germaria, Inx2 is strongly expressed in escort cells and follicle cells, both of which make close contact with germline cells. We show that inx2 function in germarial somatic cells is required for the survival of early germ cells and promotes cyst formation, probably downstream of EGFR pathway, and that inx2 function in follicle cells promotes egg chamber formation through the regulation of DE-cadherin and Bazooka (Baz) at the boundary between germ cells and follicle cells. Furthermore, genetic experiments demonstrate that inx2 interacts with the zero population growth (zpg) gene, which encodes a germline-specific gap junction protein. These results indicate a multifunctional role for Inx2 gap junctions in somatic support cells in the regulation of early germ cell survival, cyst formation and egg chamber formation. Inx2 junctions may mediate the transfer of nutrients and signal molecules between germ cells and somatic support cells, as well as play a role in the regulation of cell adhesion.
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Genetically encoded system to track histone acetylation in vivo 査読あり
Y. Sato, M. Mukai, J. Ueda, M. Muraki, T. J. Stasevich, N. Horikoshi, T. Kujirai, H. Kita, T. Kimura, S. Hira, Y. Okada, Y. Hayashi-Takanaka, C. Obuse, H. Kurumizaka, A. Kawahara, K. Yamagata, N. Nozaki, and H. Kimura
Scientific Reports 2013年
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Binding of Drosophila maternal Mamo protein to chromatin and specific DNA sequences
S. Hira, T. Okamoto, M. Fujiwara, H. Kita, S. Kobayashi and M. Mukai
Biochem. Biophys Res. Commun. 2013年
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Maternal RNAs encoding transcription factors required for germline-specific gene expression in Drosophila embryos(共著) 査読あり
J. Yatsu, M. Hayashi, M. Mukai, K. Arita, S. Shigenobu, S. Kobayashi
Int. J. Dev. Biol 52 913 - 923 2008年7月
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Maternal RNAs encoding transcription factors required for germline-specific gene expression in Drosophila embryos
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MAMO, a maternal BTB/POZ-Zn-finger protein enriched in germline progenitors is required for the production of functional eggs in Drosophila. (共著) 査読あり
M. Mukai, Y. Hayashi, Y. Kitadate, S. Shigenobu, K. Arita, S. Kobayashi
Mech. Dev. 124 570 - 583 2007年5月
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担当区分:筆頭著者
MAMO, a maternal BTB/POZ-Zn-finger protein enriched in germline progenitors is required for the production of functional eggs in Drosophila. Mech. Dev. (2007)124, 570-583.
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Expression of the core promoter factors TBP and TRF2 in Drosophila germ cells and their distinct functions in germline development 査読あり
Shoichi Nakamura, Seiji Hira, Makoto Kojima, Akane Kondo, Masanori Mukai
Development, Growth & Differentiation 62 540 - 553 2020年12月
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担当区分:責任著者 出版者・発行元:Wiley
In Drosophila, the expression of germline genes is initiated in primordial germ cells (PGCs) and is known to be associated with germline establishment. However, the transcriptional regulation of germline genes remains elusive. Previously, we found that the BTB/POZ-Zn-finger protein, Mamo, is necessary for the expression of the germline gene, vasa, in PGCs. Moreover, truncated Mamo lacking the BTB/POZ do- main (MamoAF) is a potent vasa activator. In this study, we investigated the genetic interaction between MamoAF and specific transcriptional regulators to gain insight into the transcriptional regulation of germline development. We identified a general transcription factor, TATA box binding protein (TBP)-associated factor 3 (TAF3/BIP2), and a member of the TBP-like proteins, TBP-related factor 2 (TRF2), as new genetic modifiers of MamoAF. In contrast to TRF2, TBP was found to show no genetic inter- action with MamoAF, suggesting that Trf2 has a selective function. Therefore, we focused on Trf2 expression and investigated its function in germ cells. We found that Trf2 mRNA, rather than Tbp mRNA, was preferentially expressed in PGCs during embryogenesis. Depletion of TRF2 in PGCs resulted in decreased mRNA expression of vasa. RNA interference-mediated knockdown showed that, while Trf2 is required for maintenance of germ cells, Tbp is needed for their differentiation during oogen- esis. Therefore, these results suggest that Trf2 and Tbp expression is differentially regulated in germ cells and that these factors have distinct functions in Drosophila germline development.
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Histone acetylation on Drosophila polytene chromosomes visualized by mintbody. 査読あり
Yuko Sato, M. Mukai, H Kimura
Cytologia 80 ( 4 ) 383 - 384 2015年12月
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Transcriptional regulatory regions that promote innexin2 expression in somatic support cells in the Drosophila ovary
T. Kimura, H. Kita, S. Hira, A. Kuhara, T. G. Kusakabe, M. Michinomae, S. Kobayashi, and M. Mukai
Memoirs of Konan University, Science and Engineering Series 2013年
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ショウジョウバエ始原生殖細胞中におけるRpL-22 like遺伝子の発現様式(共著) 査読あり
向 正則, 本田皓子, 味村夏芽, 今井博之,本多大輔,小林 悟
甲南大学紀要 2010年11月
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担当区分:筆頭著者
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Maternal Nanos represses hid/skl-dependent apoptosis to maintain the germ line in Drosophila embryos. (共著) 査読あり
K. Sato, Y. Hayashi, Y., Ninomiya, S. Shigenobu, K.Arita, M. Mukai, S. Kobayashi.
PNAS 104 7455 - 7460 2007年5月
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Maternal Nanos represses hid/skl-dependent apoptosis to maintain the germ line in Drosophila embryos. PNAS (2007) 104, 7455-7460.
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Expression of meiotic genes in the germline progenitors of Drosophila embryos. (共著) 査読あり
M. Mukai, Y. Kitadate, K. Arita, S. Shigenobu, S. Kobayashi
Gene Exp. Pat. 6 256 - 266 2006年7月
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担当区分:筆頭著者
Expression of meiotic genes in the germline progenitors of Drosophila embryos. Gene Exp. Pat. (2006) 6, 256-266.
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Characterization of the isoforms of MOVO zinc finger protein, a mouse homologue of Drosophila Ovo, as transcription factors. (共著) 査読あり
S. Unezaki, M. Nishizawa, E. Okuda-Ashitaka, Y. Masu, M. Mukai, S. Kobayashi, K. Sawamoto, H. Okano, S. Ito
Gene 336 47 - 58 2004年5月
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Characterization of the isoforms of MOVO zinc finger protein, a mouse homologue of Drosophila Ovo, as transcription factors. (2004) Gene 336, 47-58.
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Maternal Nanos and Pumilio regulate zygotic vasa expression autonomously in the germ-line progenitors of Drosophila melanogaster embryos. 査読あり
H. Sano, M. Mukai and S. Kobayashi
Develop. Growth Differ. 43 545 - 552 2001年5月
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Maternal Nanos and Pumilio regulate zygotic vasa expression autonomously in the germ-line progenitors of Drosophila melanogaster embryos.
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Localization of mitochondorial large ribosomal RNA in germ plasm of Xenopus embryos.(共著)
S. Kobayashi, R. Amikura, M. Mukai
Curr. Biol. 8 1117 - 1120 1999年9月
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Induction of indora expression in pole cells by the mesoderm is required for female germ-line development in Drosophila melanogaster.(共著) 査読あり
M. Mukai, M. Kashikawa and S. Kobayashi
Development 126 1023 - 1029 1999年2月
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担当区分:筆頭著者
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Requirement for a noncoding RNA in Drosophila polar granules for germ cell establishment.(共著) 査読あり
A. Nakamura, R. Amikura, M. Mukai, S. Kobayashi, Paul F. Lasko
Science 274 2075 - 2079 1996年12月
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