論文 - 日下部 岳広
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Retinal Cone Mosaic in sws1-Mutant Medaka (Oryzias latipes), A Teleost 査読あり 国際誌
Megumi Matsuo, Makoto Matsuyama, Tomoe Kobayashi, Shinji Kanda, Satoshi Ansai, Taichi Kawakami, Erika Hosokawa, Yutaka Daido, Takehiro G. Kusakabe, Kiyoshi Naruse, and Shoji Fukamachi
Investigative Opthalmology & Visual Science 63 ( 11 ) 21 - 21 2022年10月
出版者・発行元：Association for Research in Vision and Ophthalmology (ARVO)
PURPOSE: Ablation of short single cones (SSCs) expressing short-wavelength-sensitive opsin (SWS1) is well analyzed in the field of regenerative retinal cells. In contrast with ablation studies, the phenomena caused by the complete deletion of SWS1 are less well-understood. To assess the effects of SWS1 deficiency on retinal structure, we established and analyzed sws1-mutant medaka. METHODS: To visualize SWS1, a monoclonal anti-SWS1 antibody and transgenic reporter fish (Tg(sws1:mem-egfp)) were generated. We also developed a CRISPR/Cas-driven sws1-mutant line. Retinal structure of sws1 mutant was visualized using anti-SWS1, 1D4, and ZPR1 antibodies and coumarin derivatives and compared with wild type, Tg(sws1:mem-egfp), and another opsin (lws) mutant. RESULTS: Our rat monoclonal antibody specifically recognized medaka SWS1. Sws1 mutant retained regularly arranged cone mosaic as lws mutant and its SSCs had neither SWS1 nor long wavelength sensitive opsin. Depletion of sws1 did not affect the expression of long wavelength sensitive opsin, and vice versa. ZPR1 antibody recognized arrestin spread throughout double cones and long single cones in wild-type, transgenic, and sws1-mutant lines. CONCLUSIONS: Comparative observation of sws1-mutant and wild-type retinas revealed that ZPR1 negativity is not a marker for SSCs with SWS1, but SSCs themselves. Loss of functional sws1 did not cause retinal degeneration, indicating that sws1 is not essential for cone mosaic development in medaka. Our two fish lines, one with visualized SWS1 and the other lacking functional SWS1, offer an opportunity to study neural network synapsing with SSCs and to clarify the role of SWS1 in vision.
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Neuronal identities derived by misexpression of the POU IV sensory determinant in a protovertebrate 査読あり 国際共著
Prakriti Paul Chacha, Ryoko Horie, Takehiro G. Kusakabe, Yasunori Sasakura, Mona Singh, Takeo Horie, Michael Levine
Proceedings of the National Academy of Sciences 119 ( 4 ) e2118817119 - e2118817119 2022年1月
出版者・発行元：Proceedings of the National Academy of Sciences
The protovertebrate <italic>Ciona intestinalis</italic> type A (sometimes called <italic>Ciona robusta</italic>) contains a series of sensory cell types distributed across the head–tail axis of swimming tadpoles. They arise from lateral regions of the neural plate that exhibit properties of vertebrate placodes and neural crest. The sensory determinant <italic>POU IV/Brn3</italic> is known to work in concert with regional determinants, such as <italic>Foxg</italic> and <italic>Neurogenin</italic>, to produce palp sensory cells (PSCs) and bipolar tail neurons (BTNs), in head and tail regions, respectively. A combination of single-cell RNA-sequencing (scRNA-seq) assays, computational analysis, and experimental manipulations suggests that misexpression of <italic>POU IV</italic> results in variable transformations of epidermal cells into hybrid sensory cell types, including those exhibiting properties of both PSCs and BTNs. Hybrid properties are due to coexpression of <italic>Foxg</italic> and <italic>Neurogenin</italic> that is triggered by an unexpected <italic>POU IV</italic> feedback loop. Hybrid cells were also found to express a synthetic gene battery that is not coexpressed in any known cell type. We discuss these results with respect to the opportunities and challenges of reprogramming cell types through the targeted misexpression of cellular determinants.
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A single motor neuron determines the rhythm of early motor behavior in Ciona 査読あり
Taichi Akahoshi, Madoka K. Utsumi, Kouhei Oonuma, Makoto Murakami, Takeo Horie, Takehiro G. Kusakabe, Kotaro Oka, Kohji Hotta
Science Advances 7 ( 50 ) 2021年12月
出版者・発行元：American Association for the Advancement of Science (AAAS)
Single motor neuron regulates rhythmic tail flick in prehatching
<italic>Ciona</italic>
embryo. -
Kouhei Oonuma, Maho Yamamoto, Naho Moritsugu, Nanako Okawa, Megumi Mukai, Miku Sotani, Shuto Tsunemi, Haruka Sugimoto, Eri Nakagome, Yuichi Hasegawa, Kotaro Shimai, Takeo Horie, Takehiro G. Kusakabe
Frontiers in Cell and Developmental Biology 9 ( 704367 ) 704367 - 704367 2021年5月
共著
担当区分：最終著者, 責任著者
In vertebrate embryos, dorsal midline tissues, including the notochord, the prechordal plate, and the floor plate, play important roles in patterning of the central nervous system, somites, and endodermal tissues by producing extracellular signaling molecules, such as Sonic hedgehog (Shh). In Ciona, hedgehog.b, one of the two hedgehog genes, is expressed in the floor plate of the embryonic neural tube, while none of the hedgehog genes are expressed in the notochord. We have identified a cis-regulatory region of hedgehog.b that was sufficient to drive a reporter gene expression in the floor plate. The hedgehog.b cis-regulatory region also drove ectopic expression of the reporter gene in the endodermal strand, suggesting that the floor plate and the endodermal strand share a part of their gene regulatory programs. The endodermal strand occupies the same topographic position of the embryo as does the vertebrate hypochord, which consists of a row of single cells lined up immediately ventral to the notochord. The hypochord shares expression of several genes with the floor plate, including Shh and FoxA, and play a role in dorsal aorta development. Whole-embryo single-cell transcriptome analysis identified a number of genes specifically expressed in both the floor plate and the endodermal strand in Ciona tailbud embryos. A Ciona FoxA orthologue FoxA.a is shown to be a candidate transcriptional activator for the midline gene battery. The present findings suggest an ancient evolutionary origin of a common developmental program for the midline structures in Olfactores.
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Kouhei Oonuma, Takehiro G. Kusakabe
Development dev.198754 ( 12 ) dev198754 2021年5月
共著
担当区分：最終著者
The Ciona larva has served as a unique model for understanding the development of dopaminergic cells at single-cell resolution due to the exceptionally small number of neurons in its brain and its fixed cell lineage during embryogenesis. A recent study suggested that the transcription factors Fer2 and Meis directly regulate the dopamine synthesis genes in Ciona, but the dopaminergic cell lineage and the gene regulatory networks that control the development of dopaminergic cells have not been fully elucidated. Here, we reveal that the dopaminergic cells in Ciona are derived from a bilateral pair of cells called a9.37 cells at the center of the neural plate. The a9.37 cells divide along the anterior-posterior axis, and all of the descendants of the posterior daughter cells differentiate into the dopaminergic cells. We show that the MAPK pathway and the transcription factor Otx are required for the expression of Fer2 in the dopaminergic cell lineage. Our findings establish the cellular and molecular framework for fully understanding the commitment to dopaminergic cells in the simple chordate brain.
DOI： 10.1242/dev.198754
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Nanako Okawa, Kotaro Shimai, Kohei Ohnishi, Masamichi Ohkura, Junichi Nakai, Takeo Horie, Atsushi Kuhara, Takehiro G Kusakabe
Scientific reports 10 ( 1 ) 18590 - 18590 2020年10月
共著
担当区分：最終著者, 責任著者
Tunicate larvae have a non-reproductive gonadotropin-releasing hormone (GnRH) system with multiple ligands and receptor heterodimerization enabling complex regulation. In Ciona intestinalis type A larvae, one of the gnrh genes, gnrh2, is conspicuously expressed in the motor ganglion and nerve cord, which are homologous structures to the hindbrain and spinal cord, respectively, of vertebrates. The gnrh2 gene is also expressed in the proto-placodal sensory neurons, which are the proposed homologue of vertebrate olfactory neurons. Tunicate larvae occupy a non-reproductive dispersal stage, yet the role of their GnRH system remains elusive. In this study, we investigated neuronal types of gnrh2-expressing cells in Ciona larvae and visualized the activity of these cells by fluorescence imaging using a calcium sensor protein. Some cholinergic neurons and dopaminergic cells express gnrh2, suggesting that GnRH plays a role in controlling swimming behavior. However, none of the gnrh2-expressing cells overlap with glycinergic or GABAergic neurons. A role in motor control is also suggested by a relationship between the activity of gnrh2-expressing cells and tail movements. Interestingly, gnrh2-positive ependymal cells in the nerve cord, known as a kind of glia cells, actively produced Ca2+ transients, suggesting that active intercellular signaling occurs in the glia cells of the nerve cord.
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Spatio-temporal regulation of Rx and mitotic patterns shape the eye-cup of the photoreceptor cells in Ciona 査読あり
Oonuma, K., Kusakabe, T. G.
Developmental Biology 445 ( 2 ) 245 - 255 2019年1月
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Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics 査読あり
Horie, T., Horie, R., Chen, K., Cao, C., Nakagawa, M., Kusakabe, T. G., Satoh, N., Sasakura, Y., Levine, M.
Genes & Development 32 ( 19-20 ) 1297 - 1302 2018年10月
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The Use of cis-Regulatory DNAs as Molecular Tools
Kotaro Shimai and Takehiro Kusakabe
Advances in Experimental Medicine and Biology 1029 49 - 68 2018年3月
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ANISEED 2017: extending the integrated ascidian database to the exploration and evolutionary comparison of genome-scale datasets 査読あり
Brozovic, M., Dantec, C., Dardaillon, J., Dauga, D., Faure, E., Gineste, M., Louis, A., Naville, M., Nitta, K.R., Piette, J., Reeves, W., Scornavacca, C., Simion, P., Vincentelli, R., Bellec, M., Aicha, S.B., Fagotto, M., Guéroult-Bellone, M., Haeussler, M., Jacox, E., Lowe, E.K., Mendez, M., Roberge, A., Stolfi, A., Yokomori, R., Brown, C.T., Cambillau, C., Christiaen, L., Delsuc, F., Douzery, E., Dumollard, R,, Kusakabe, T., Nakai, K., Nishida, H., Satou, Y., Swalla, B., Veeman, M., Volff, J.N., Lemaire, P.
Nucleic Acids Research 46 ( D1 ) D718 - D725 2018年1月
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DOI： 10.1093/nar/gkx1108
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Constrained vertebrate evolution by pleiotropic genes 査読あり
Hu, H., Uesaka, M., Guo, S., Shimai, K., Lu, T.-M. Li, F., Fujimoto, S., Ishikawa, M., Liu, S., Sasagawa, Y., Zhang, G., Kuratani, S., Yu, J.-K. Kusakabe, T. G., Khaitovich, P., Irie, N., the EXPANDE Consortium
Nature Ecology and Evolution 1 (11), 1722-1730 1 ( 11 ) 1722 - 1730 2017年11月
共著
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Evolutionary steps involving counterion displacement in a tunicate opsin 査読あり
Kojima, K., Yamashita, T., Imamoto, Y., Kusakabe, T. G., Tsuda, M., Shichida, Y.
Proceedings of the National Academy of Sciences of the United States of America 114 ( 23 ) 6028 - 6033 2017年6月
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Identifying vertebrate brain prototypes in deuterostomes
Takehiro G. Kusakabe
Brain Evolution by Design: From Neural Origin to Cognitive Architecture 153 - 186 2017年2月
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Revised lineage of larval photoreceptor cells in Ciona reveals archetypal collaboration between neural tube and neural crest in sensory organ formation 査読あり
Oonuma, K., Tanaka, M., Nishitsuji, K., Kato, Y., Shimai, K., Kusakabe, T. G.
Developmental Biology 420 ( 1 ) 178 - 185 2016年12月
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Genome-wide identification and characterization of transcription start sites and promoters in the tunicate Ciona intestinalis 査読あり
Yokomori, R., Shimai, K., Nishitsuji, K., Suzuki, Y., Kusakabe, T. G. and Nakai K.
Genome Research 26 ( 1 ) 140 - 150 2016年1月
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The pre-vertebrate origins of neurogenic placodes 査読あり
Abitua, P. A., Gainous, T. B., Kaczmarczyk, A. N., Winchell, C. J., Hudson, C., Kamata, K., Nakagawa, M., Tsuda, M., Kusakabe, T. G. and Levine, M.
Nature 524 ( 7566 ) 462 - 465 2015年8月
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DOI： 10.1038/nature14657
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シロイヌナズナにおけるスフィンゴイドキナーゼ（LCBK1）の形質転換体の作製
柳川大樹，田中修，日下部岳広，本多大輔，後藤彩子，今井博之
甲南大学紀要 理工学編 62 ( 1 ) 1 - 18 2015年
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Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis 査読あり
Kamiya, C., Ohta, N., Ogura, Y., Yoshida, K., Horie, T., Kusakabe, T. G., Satake, H., and Sasakura, Y.
Developmental Dynamics 243 ( 12 ) 1524 - 1535 2014年12月
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DOI： 10.1002/dvdy.24176
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Transcriptional co-regulation of evolutionarily conserved microRNA/cone opsin gene pairs: Implications for photoreceptor subtype specification 査読あり
Daido, Y., Hamanishi, S., and Kusakabe, T. G.
Developmental Biology 392 ( 1 ) 117 - 129 2014年8月
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Characterization of the compact bicistronic microRNA precursor, miR-1/miR-133, expressed specifically in Ciona muscle tissues 査読あり
Kusakabe, R., Tani, S., Nishitsuji, K., Shindo, M., Okamura, K., Miyamoto, Y., Nakai, K., Suzuki, Y., Kusakabe, T. G., and Inoue, K.
Gene Expression Patterns 13 ( 1-2 ) 43 - 53 2013年2月
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Genome Structure, Functional Genomics, and Proteomics in Ascidians
Yasunori Sasakura, Nicolas Sierro, Kenta Nakai, Kazuo Inaba, and Takehiro G. Kusakabe
Genome Mapping and Genomics in Laboratory Animals. Genome Mapping and Genomics in Animals 4 87 - 132 2013年1月
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Transcriptional regulatory regions that promote innexin2 expression in somatic support cells in the Drosophila ovary
Kimura, T., Kita, H., Hira, S., Kuhara, A., Kusakabe, T. G., Michinomae, M., Kobayashi, S., Mukai, M.
Memoirs of Konan University, Science and Engineering Series 60 ( 1 ) 1 - 9 2013年
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DOI： 10.14990/00000186
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Determination of D-serine in several model organisms used for metabolic, developmental and/or genetic researches by liquid chromatography/fluorescence detection and tandem mass spectrometry
Syuto, M., Kusakabe, T., Honda, D., Watanabe, Y., Takeda, K., Tanaka, O., Imai, H.
Memoirs of Konan University, Science and Engineering Series 60 ( 1 ) 11 - 19 2013年
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DOI： 10.14990/00000187
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Cis-acting transcriptional repression establishes a sharp boundary in chordate embryos 査読あり
Imai, K. S., Daodo, Y., Kusakabe, T. G., and Satou, Y.
Science 337 ( 6097 ) 964 - 967 2012年8月
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A conserved non-reproductive GnRH system in chordates 査読あり
Kusakabe, T. G., Sakai, T., Aoyama, M., Kitajima, Y., Miyamoto, Y., Takigawa, T., Daido, Y., Fujiwara, K., Terashima, Y., Sugiuchi, Y., Matassi, G., Yagisawa, H., Park, M. K., Satake, H., and Tsuda, M.
PLoS One 7 ( 7 ) e41955 - e41955 (12 pages) 2012年7月
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Monoaminergic modulation of photoreception in ascidian: Evidence for a proto-hypothalamo-retinal territory 査読あり
Razy-Krajka, F., Brown, E. R., Horie, T., Callebert, J., Sasakura, Y., Joly, J. S. Kusakabe, T. G., and Vernier, P.
BMC Biology 10 ( 45 ) 1 - 14 2012年5月
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Evidence for differential regulation of GnRH signaling via heterodimerization among GnRH receptor paralogs in the protochordate, Ciona intestinalis 査読あり
Sakai, T., Aoyama, M., Kawada, T., Kusakabe, T., Tsuda, M., and Satake, H.
Endocrinology 153 ( 4 ) 1841 - 1849E 2012年4月
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DOI： 10.1210/en.2011-1668
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Cell lineage and cis-regulation for a unique GABAergic/glycinergic neuron type in the larval nerve cord of the ascidian Ciona intestinalis 査読あり
Nishitsuji, K., Horie, T., Ichinose, A., Sasakura, Y., Yasuo, H., and Kusakabe, T. G.
Development, Growth and Differentiation 54 ( 2 ) 177 - 186 2012年2月
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Profiling ascidian promoters as the primordial type of vertebrate promoter 査読あり
Okamura, K., Yamashita, R., Takimoto, N., Nishitsuji, K., Suzuki, Y., Kusakabe, T. G., and Nakai, K.
BMC Genomics 12 ( Suppl 3 ) S7 - S7 (14 pages) 2011年11月
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COQ6 mutations in human patients produce nephrotic syndrome with sensorineural deafness 査読あり
Heeringa, S. F., Chernin, G., Chaki, M., Zhou, W., Sloan, A. J., Ji, Z., Xie, L. X., Salviati, L., Hurd, T. W., Vega-Warner, V., Killen, P. D., Raphael, Y., Ashraf, S., Ovunc, B., Schoeb, D. S., McLaughlin, H. M., Airik, R., Vlangos, C. N., Gbadegesin, R., Hinkes, B., Saisawat, P., Trevisson, E., Doimo, M., Casarin, A., Pertegato, V., Giorgi, G., Prokisch, H., Rötig, A., Nürnberg, G., Becker, C., Wang, S., Ozaltin, F., Topaloglu, R., Bakkaloglu, A., Bakkaloglu, S. A., Müller, D., Beissert, A., Mir, S., Berdeli, A., Varpizen, S., Zenker, M., Matejas, V., Santos-Ocaña, C., Navas, P., Kusakabe, T., Kispert, A., Akman, S., Soliman, N. A., Krick, S., Mundel, P., Reiser, J., Nürnberg, P., Clarke, C. F., Wiggins, R. C., Faul, C., and Hildebrandt, F.
Journal of Clinical Investigation 121 ( 5 ) 2013 - 2024 2011年5月
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DOI： 10.1172/JCI45693
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Cross-validated methods for promoter/transcription start site mapping in SL trans-spliced genes, established using the Ciona intestinalis troponin I gene 査読あり
Khare, P., Mortimer, S., Cleto, C., Okamura, K., Suzuki, Y., Kusakabe, T., Nakai, K., Meedel, T., and Hastings, K.
Nucleic Acids Research 39 ( 7 ) 2638 - 2648 2011年4月
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DOI： 10.1093/nar/gkq1151
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Ependymal cells of chordate larvae are stem-like cells that form the adult nervous system 査読あり
Horie, T., Shinki, R., Ogura, Y., Kusakabe, T. G., Satoh, N., and Sasakura, Y.
Nature 469 ( 7331 ) 525 - 528 2011年1月
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DOI： 10.1038/nature09631
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The ANISEED database: Digital representation, formalization, and elucidation of a chordate developmental program 査読あり
Tassy, O., Dauga, D., Daian, F., Sobral, D., Robin, F., Khoueiry, P., Salgado, D., Fox, V., Caillol, D., Schiappa, R., Laporte, B., Rios, A., Luxardi, G., Kusakabe, T., Joly, J.-S., Darras, S., Christiaen, L., Contensin, M., Auger, H., Lamy, C., Hudson, C., Rothbächer, U., Gilchrist, M., Makabe, K. W., Hotta, K., Fujiwara, S., Satoh, N., Satou, Y., and Lemaire, P.
Genome Research 20 ( 10 ) 1459 - 1468 2010年10月
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Functional diversity of signaling pathways through G protein-coupled receptor heterodimerization with a species-specific orphan receptor subtype 査読あり
Sakai, T., Aoyama, M., Kusakabe, T., Tsuda, M., and Satake, H.
Molecular Biology and Evolution 27 ( 5 ) 1097 - 1196 2010年5月
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Individuals with mutations in XPNPEP3, which encodes a mitochondrial protein, develop a nephronophthisis-like nephropathy 査読あり
O'Toole, J. F., Liu, Y., Davis, E. E., Westlake, C. J., Attanasio, M., Otto, E. A., Seelow, D., Nurnberg, G., Becker, C., Nuutinen, M., Kärppä, M., Ignatius, J., Uusimaa, J., Pakanen, S., Jaakkola, E., van den Heuvel, L. P., Fehrenbach, H., Wiggins, R., Goyal, M., Zhou, W., Wolf, M. T., Wise, E., Helou, J., Allen, S. J., Murga-Zamalloa, C. A., Ashraf, S., Chaki, M., Heeringa, S., Chernin, G., Hoskins, B. E., Chaib, H., Gleeson, J., Kusakabe, T., Suzuki, T., Isaac, R. E., Quarmby, L. M., Tennant, B., Fujioka, H., Tuominen, H., Hassinen, I., Lohi, H., van Houten, J. L., Rotig, A., Sayer, J. A., Rolinski, B., Freisinger, P., Madhavan, S. M., Herzer, M., Madignier, F., Prokisch, H., Nurnberg, P., Jackson, P. K., Khanna, H., Katsanis, N., and Hildebrandt, F.
Journal of Clinical Investigation 120 ( 3 ) 791 - 802 2010年3月
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DOI： 10.1172/JCI40076
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Distinctive expression patterns of Hedgehog pathway genes in the Ciona intestinalis 査読あり
Islam, A. F. M. T., Moly, P. K., Miyamoto, Y., and Kusakabe, T. G.
Zoological Science 27 ( 2 ) 181 - 190 2010年2月
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DOI： 10.2108/zsj.27.84
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Simple motor system of the ascidian larva: neuronal complex comprising putative cholinergic neurons and GABAergic/glycinergic neurons 査読あり
Horie, T., Nakagawa, M., Sasakura, Y., Kusakabe, T. G., and Tsuda, M.
Zoological Science 27 ( 2 ) 84 - 90 2010年2月
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DOI： 10.2108/zsj.27.181
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Evolution and the origin of the visual retinoid cycle in vertebrates 査読あり
Kusakabe, T. G., Takimoto, N., Jin, M., and Tsuda, M.
Philosophical Transactions of the Royal Society B 364 ( 1531 ) 2897 - 2910 2009年10月
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Tube formation by complex cellular processes in Ciona intestinalis notochord 査読あり
Dong, B., Horie, T., Denker, E., Kusakabe, T., Tsuda, M., Smith, W. C., and Jiang, D.
Developmental Biology 330 ( 2 ) 237 - 249 2009年6月
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Cell type and function of neurons in the ascidian nervous system 査読あり
Horie, T., Sasakura, Y., Nakagawa, M., and Kusakabe, T. G.
Development, Growth & Differentiation 51 ( 3 ) 207 - 220 2009年4月
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Pigmented and nonpigmented ocelli in the brain vesicle of the ascidian larva 査読あり
Horie, T., Sakurai, D., Ohtsuki, H., Terakita, A., Shichida, Y., Usukura, J., Kusakabe, T., and Tsuda, M.
Journal of Comparative Neurology 509 ( 1 ) 88 - 102 2008年7月
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DOI： 10.1002/cne.21733
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Functions of a GnRH receptor heterodimer of the ascidian, Ciona intestinalis 査読あり
Sakai, T., Aoyama, M., Kusakabe, T., Tsuda, M., and Satake, H.
Acta Biologica Hungarica 59 ( Suppl. ) 241 - 243 2008年6月
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Glutamatergic networks in the Ciona intestinalis larva 査読あり
Horie, T., Kusakabe, T., and Tsuda, M.
Journal of Comparative Neurology 508 ( 2 ) 249 - 263 2008年5月
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DOI： 10.1002/cne.21678
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Markov chain-based promoter structure modeling for tissue-specific expression pattern prediction 査読あり
Vandenbon, A., Miyamoto, Y., Takimoto, N., Kusakabe, T. and Nakai, K.
DNA Research 15 ( 1 ) 3 - 11 2008年2月
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Comparative genomics identifies a cis-regulatory module that activates transcription in specific subsets of neurons in Ciona intestinalis larvae 査読あり
Yoshida, R., Horie, T., Tsuda, M. and Kusakabe, T. G.
Development, Growth and Differentiation 48 ( 8 ) 657 - 667 2007年10月
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Origin of the vertebrate visual cycle 査読あり
Takimoto, N., Kusakabe, T., and Tsuda, M.
Photochemistry and Photobiology 83 ( 2 ) 242 - 247 2007年3月
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Photoreceptive systems in ascidians 査読あり
Kusakabe, T. and Tsuda, M.
Photochemistry and Photobiology 83 ( 2 ) 248 - 252 2007年3月
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Origin of the vertebrate visual cycle: III. Distinct distribution of RPE65 and β-carotene 15,15'-monooxygenase homologues in Ciona intestinalis 査読あり
Takimoto, N., Kusakabe, T., Horie, T., Miyamoto, Y., and Tsuda, M.
Photochemistry and Photobiology 82 ( 6 ) 1468 - 1474 2006年11月
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The centrosomal protein nephrocystin-6 is mutated in Joubert syndrome and activates transcription factor ATF4 査読あり
Sayer, J.A., Otto, E.A., O'toole, J.F., Nurnberg, G., Kennedy, M.A., Becker, C., Hennies, H.C., Helou, J., Attanasio, M., Fausett, B.V., Utsch, B., Khanna, H., Liu, Y., Drummond, I., Kawakami, I., Kusakabe, T., Tsuda, M., Ma, L., Lee, H., Larson, R.G., Allen, S.J., Wilkinson, C.J., Nigg, E.A., Shou, C., Lillo, C., Williams, D.S., Hoppe, B., Kemper, M.J., Neuhaus, T., Parisi, M.A., Glass, I.A., Petry, M., Kispert, A., Gloy, J., Ganner, A., Walz, G., Zhu, X., Goldman, D., Nurnberg, P., Swaroop, A., Leroux, M.R., and Hildebrandt, F.
Nature Genetics 38 ( 6 ) 674 - 681 2006年6月
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DOI： 10.1038/ng1786
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DBTGR: a database of tunicate promoters and their regulatory elements 査読あり
Sierro, N., Kusakabe, T., Park, K.-J., Yamashita, R., Kinoshita, K., and Nakai, K.
Nucleic Acids Research 34 D552 - D555 2006年1月
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DOI： 10.1093/nar/gkj064
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Evolution of constrained GnRH ligand conformation and receptor sensitivity 査読あり
Barran, P.E., Roeske, R.W., Pawson, A.J., Sellar, R., Bowers, M.T., Morgan, K., Lu, Z.-L., Tsuda, M., Kusakabe, T., and Millar, R.P.
Journal of Biological Chemistry 280 ( 46 ) 38568 - 38575 2005年11月
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Nephrocystin-5, a ciliary IQ domain protein, is mutated in Senior-Loken syndrome and interacts with RPGR and calmodulin 査読あり
Otto, E.A., Loeys, B., Khanna, H., Hellemans, J., Sudbrak, R., Fan, S., Muerb, U., O'Toole, J.F., Helou, J., Attanasio, M., Utsch, B., Sayer, J.A., Lillo, C., Jimeno, D., Coucke, P., De Paepe, A., Reinhardt, R., Klages, S., Tsuda, M., Kawakami, I., Kusakabe, T., Omran, H., Imm, A., Tippens, M., Raymond, P.A., Hill, J., Beales, P., He, S., Kispert, A., Margolis, B., Williams, D.S., Swaroop, A., and Hildebrandt, F.
Nature Genetics 37 ( 3 ) 282 - 288 2005年3月
共著
DOI： 10.1038/ng1520
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Decoding cis-regulatory systems in ascidians 査読あり
Kusakabe, T.
Zoological Science 22 ( 2 ) 129 - 146 2005年2月
単著
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Computational discovery of DNA motifs associated with cell type-specific gene expression in Ciona 査読あり
Kusakabe, T., Yoshida, R., Ikeda, Y., and Tsuda, M.
Developmental Biology 276 ( 2 ) 129 - 146 2004年12月
共著
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Localization of mRNAs encoding alpha and beta subunits of soluble guanylyl cyclase in the brain of rainbow trout: comparison with the distribution of neuronal nitric oxide synthase 査読あり
Ando, H., Shi, Q., Kusakabe, T., Ohya, T., Suzuki, N., and Urano, A.
Brain Research 1013 ( 1 ) 13 - 29 2004年7月
共著
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Identification of neuron-specific promoters in Ciona intestinalis 査読あり
Yoshida, R., Sakurai, D., Horie, T., Kawakami, I., Tsuda, M., and Kusakabe, T.
Genesis 39 ( 2 ) 130 - 140 2004年6月
共著
DOI： 10.1002/gene.20032
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原索動物ホヤの筋肉アクチン遺伝子の構造と発現調節
日下部岳広
2004年3月
単著
-
Structure, expression, and cluster organization of genes encoding gonadotropin-releasing hormone receptors found in the neural complex of the ascidian Ciona intestinalis 査読あり
Kusakabe, T., Mishima, S., Shimada, I., Kitajima, Y., and Tsuda, M.
Gene 322 77 - 84 2003年12月
共著
-
Origin of the vertebrate visual cycle. II. Visual cycle proteins are localized in whole brain including photoreceptor cells of a primitive chordate 査読あり
Tsuda, M., Kusakabe, T., Iwamoto, H., Horie, T., Nakashima, Y., Nakagawa, M., and Okunou, K.
Vision Research 43 ( 28 ) 3045 - 3053 2003年12月
共著
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Targeted knockdown of an opsin gene inhibits the swimming behaviour photoresponse of ascidian larvae 査読あり
Inada, K., Horie, T., Kusakabe, T., and Tsuda, M.
Neuroscience Letters 347 ( 3 ) 167 - 170 2003年8月
共著
-
Origin of the vertebrate visual cycle: genes encoding retinal photoisomerase and two putative visual cycle proteins are expressed in whole brain of a primitive chordate 査読あり
Nakashima, Y., Kusakabe, T., Kusakabe, R., Terakita, A., Shichida, Y., and Tsuda, M.
Journal of Comparative Neurology 460 ( 2 ) 180 - 190 2003年5月
共著
DOI： 10.1002/cne.10645
-
Central nervous system-specific expression of G protein α-subunits in the ascidian Ciona intestinalis 査読あり
Yoshida, R., Kusakabe, T., Kamatani, M., Daitoh, M., and Tsuda, M.
Zoological Science 19 ( 10 ) 1079 - 1088 2002年10月
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Phototransduction and visual cycle in the ascidian tadpole larva 査読あり
Kusakabe, T., Nakashima, Y., Kusakabe, R., Takeo, H., Kawakami, I., Yoshida, R., Inada, K., Nakagawa, M., and Tsuda, M.
Journal of Photoscience 9 ( 2 ) 37 - 40 2002年8月
共著
-
Ciona intestinalis cDNA projects: EST analyses and gene expression profiles during embryogenesis 査読あり
Satou, Y., Takatori, N., Fujiwara, S., Nishikata, T., Saiga, H., Kusakabe, T., Shin-i, T., Kohara, Y., and Satoh, N.
Gene 287 ( 1-2 ) 83 - 96 2002年4月
共著
-
Gene expression profiles in tadpole larvae of Ciona intestinalis
Kusakabe, T., Yoshida, R., Kawakami, I., Kusakabe, R., Mochizuki, Y., Yamada, L., Shin-i, T., Kohara, K., Satoh, N., Tsuda, M., and Satou, Y.
Developmental Biology 242 ( 2 ) 188 - 203 2002年2月
共著
-
Ci-opsin1, a vertebrate-type opsin gene, expressed in the larval ocellus of the ascidian Ciona intestinalis 査読あり
Kusakabe, T., Kusakabe, R., Kawakami, K., Satou, Y., Satoh, N., and Tsuda, M.
FEBS Letters 506 ( 1 ) 69 - 72 2001年9月
共著
-
A cis-regulatory element essential for photoreceptor-specific expression of a medaka retinal guanylyl cyclase gene 査読あり
Kusakabe, T. and Suzuki, N.
Development Genes and Evolution 211 ( 3 ) 145 - 149 2001年3月
共著
-
Photoreceptors and olfactory cells express the same retinal guanylyl cyclase isoform in medaka: visualization by promoter transgenics 査読あり
Kusakabe, T. and Suzuki, N.
FEBS Letters 483 ( 2-3 ) 143 - 148 2000年10月
共著
-
A detailed linkage map of medaka, Oryzias latipes: comparative genomics and genome evolution 査読あり
Naruse, K., Fukamachi, S., Mitani, H., Kondo, M., Matsuoka, T., Kondo, S., Hamamura, N., Morita, Y., Hasegawa, K., Nishigaki, R., Shimada, A., Wada, H., Kusakabe, T., Suzuki, N., Kinoshita, M., Kanamori, A., Terado, T., Kimura, H., Nonaka, M., and Shima, A.
Genetics 154 ( 4 ) 1773 - 1784 2000年4月
共著
-
The guanylyl cyclase family in medaka fish Oryzias latipes 査読あり
Kusakabe, T. and Suzuki, N.
Zoological Science 17 ( 2 ) 131 - 140 2000年2月
-
In vivo analysis of two striated muscle actin promoters reveals combinations of multiple regulatory modules required for skeletal and cardiac muscle-specific gene expression 査読あり
Kusakabe, R., Kusakabe, T., and Suzuki, N.
International Journal of Developmental Biology 43 ( 6 ) 541 - 554 1999年9月
共著
-
Tandem organization of medaka fish soluble guanylyl cyclase α1 and β1 subunit genes: implications for coordinated transcription of two subunit genes 査読あり
Mikami, T., Kusakabe, T., and Suzuki, N.
Journal of Biological Chemistry 274 ( 26 ) 18567 - 18573 1999年6月
共著
-
Evolution of the ascidian anural larva: evidence from embryos and molecules 査読あり
Jeffery, W. R., Billie J. Swalla, B. J., Ewing, N., and Kusakabe, T.
Molecular Biology and Evolution 16 ( 5 ) 646 - 654 1999年5月
共著
-
Sequence analysis of cDNA and genomic DNA, and mRNA expression of the medaka fish homolog of the mammalian guanylyl cyclase C 査読あり
Mantoku, T., Muramatsu, R., Nakauchi, M., Yamagami, S., Kusakabe, T., and Suzuki, N.
Journal of Biochemistry 125 ( 3 ) 476 - 486 1999年3月
共著
-
Genomic organization and evolution of actin genes in the amphioxus Branchiostoma belcheri and Branchiostoma floridae 査読あり
Kusakabe, R., Satoh, N., Holland, L. Z., and Kusakabe, T.
Gene 227 ( 1 ) 1 - 10 1999年2月
共著
-
Sequence analysis of cDNAs and genomic DNA, and mRNA expression of the medaka fish homolog of the mammalian guanylyl cyclase C (共著)
日下部 岳広
Journal of Biochemistry 125 ( 3 ) 476 - 486 1999年
単著
-
Molecular cloning of cDNAs and expression of mRNAs encoding a and b subunits of soluble guanylyl cyclase from medaka fish Oryzias latipes 査読あり
Mikami, T., Kusakabe, T., and Suzuki, N.
European Journal of Biochemistry 253 ( 1 ) 42 - 48 1998年4月
-
Ascidian actin genes: developmental regulation of gene expression and molecular evolution 査読あり
Kusakabe, T.
Zoological Science 14 ( 5 ) 707 - 718 1997年10月
単著
-
Differential gene expression and intracellular mRNA localization of amphioxus actin isoforms throughout development: implications for conserved mechanisms of chordate development 査読あり
Kusakabe, R., Kusakabe, T., Satoh, N., Holland, N. D., and Holland, L. Z.
Development Genes and Evolution 207 ( 4 ) 203 - 215 1997年10月
共著
-
Primary structure and differential gene expression of three membrane forms of guanylyl cyclases found in the eye of the teleost Oryzias latipes 査読あり
Seimiya, M., Kusakabe, T., and Suzuki, N.
Journal of Biological Chemistry 272/37, 23407-23417 272 ( 37 ) 23407 - 23417 1997年9月
共著
-
Tunic cuticular protrusions in ascidians (Chordata, Tunicata): a perspective of their character-state distribution 査読あり
Hirose, E., Lambert, G., Kusakabe, T., and Nishikawa, T.
Zoological Science 14 ( 4 ) 683 - 689 1997年4月
共著
DOI： 10.2108/zsj.14.683
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Evolution of chordate actin genes: evidence from genomic organization and amino acid sequences 査読あり
Kusakabe, T., Araki, I., Satoh, N., and Jeffery, W. R.
Journal of Molecular Evolution 44 ( 3 ) 289 - 298 1997年3月
共著
DOI： 10.1007/PL00006146
-
Predominant expression of a cytoskeletal actin gene in mesenchyme cells during embryogenesis of the ascidian Halocynthia roretzi 査読あり
Araki, I., Tagawa, K., Kusakabe, T., and Satoh, N.
Development, Growth & Differentiation 38 ( 4 ) 401 - 411 1996年8月
-
Mechanism of an evolutionary change in muscle cell differentiation in ascidians with different modes of development 査読あり
Kusakabe, T., Swalla, B. J., Satoh, N., and Jeffery, W. R.
Developmental Biology 174 ( 2 ) 379 - 392 1996年3月
共著
-
Timing of initiation of muscle-specific gene expression in the ascidian embryo precedes that of developmental fate restriction in lineage cells 査読あり
Satou, Y., Kusakabe, T., Araki, I., and Satoh, N.
Development, Growth & Differentiation 37 ( 3 ) 319 - 327 1995年6月
-
Coexpression and promoter function in two muscle actin gene complexes of different structural organization in the ascidian Halocynthia roretzi 査読あり
Kusakabe, T., Hikosaka, A., and Satoh, N.
Developmental Biology 169 ( 2 ) 461 - 472 1995年6月
共著
-
Expression of larval-type muscle actin-encoding genes in the ascidian Halocynthia roretzi 査読あり
Kusakabe, T.
Gene 153 ( 2 ) 215 - 218 1995年2月
単著
-
Short upstream sequences associated with the muscle-specific expression of an actin gene in ascidian embryos 査読あり
Hikosaka, A., Kusakabe, T., and Satoh, N.
Developmental Biology 166 ( 2 ) 763 - 769 1994年12月
共著
-
Introduction and expression of recombinant genes in ascidian embryos 査読あり
Hikosaka, A., Kusakabe, T., Satoh, N., and Makabe, K. W.
Development, Growth & Differentiation 34 ( 6 ) 627 - 634 1992年12月
-
Tunicate muscle actin genes: structure and organization as a gene cluster 査読あり
Kusakabe, T., Makabe, K. W., and Satoh, N.
Journal of Molecular Biology 227 ( 3 ) 955 - 960 1992年10月
共著
-
Temporal and spatial expression of a muscle actin gene during embryogenesis of the ascidian Halocynthia roretzi 査読あり
Kusakabe, T., Suzuki, J., Saiga, H., Jeffery, W. R., Makabe, K. W., and Satoh, N.
Development, Growth & Differentiation 33 ( 3 ) 227 - 234 1991年6月
共著