Papers - KUSAKABE Takehiro
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Retinal Cone Mosaic in sws1-Mutant Medaka (Oryzias latipes), A Teleost Reviewed International journal
Megumi Matsuo, Makoto Matsuyama, Tomoe Kobayashi, Shinji Kanda, Satoshi Ansai, Taichi Kawakami, Erika Hosokawa, Yutaka Daido, Takehiro G. Kusakabe, Kiyoshi Naruse, and Shoji Fukamachi
Investigative Opthalmology & Visual Science 63 ( 11 ) 21 - 21 2022.10
Publisher：Association for Research in Vision and Ophthalmology (ARVO)
PURPOSE: Ablation of short single cones (SSCs) expressing short-wavelength-sensitive opsin (SWS1) is well analyzed in the field of regenerative retinal cells. In contrast with ablation studies, the phenomena caused by the complete deletion of SWS1 are less well-understood. To assess the effects of SWS1 deficiency on retinal structure, we established and analyzed sws1-mutant medaka. METHODS: To visualize SWS1, a monoclonal anti-SWS1 antibody and transgenic reporter fish (Tg(sws1:mem-egfp)) were generated. We also developed a CRISPR/Cas-driven sws1-mutant line. Retinal structure of sws1 mutant was visualized using anti-SWS1, 1D4, and ZPR1 antibodies and coumarin derivatives and compared with wild type, Tg(sws1:mem-egfp), and another opsin (lws) mutant. RESULTS: Our rat monoclonal antibody specifically recognized medaka SWS1. Sws1 mutant retained regularly arranged cone mosaic as lws mutant and its SSCs had neither SWS1 nor long wavelength sensitive opsin. Depletion of sws1 did not affect the expression of long wavelength sensitive opsin, and vice versa. ZPR1 antibody recognized arrestin spread throughout double cones and long single cones in wild-type, transgenic, and sws1-mutant lines. CONCLUSIONS: Comparative observation of sws1-mutant and wild-type retinas revealed that ZPR1 negativity is not a marker for SSCs with SWS1, but SSCs themselves. Loss of functional sws1 did not cause retinal degeneration, indicating that sws1 is not essential for cone mosaic development in medaka. Our two fish lines, one with visualized SWS1 and the other lacking functional SWS1, offer an opportunity to study neural network synapsing with SSCs and to clarify the role of SWS1 in vision.
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Neuronal identities derived by misexpression of the POU IV sensory determinant in a protovertebrate Reviewed International coauthorship
Prakriti Paul Chacha, Ryoko Horie, Takehiro G. Kusakabe, Yasunori Sasakura, Mona Singh, Takeo Horie, Michael Levine
Proceedings of the National Academy of Sciences 119 ( 4 ) e2118817119 - e2118817119 2022.1
Publisher：Proceedings of the National Academy of Sciences
The protovertebrate <italic>Ciona intestinalis</italic> type A (sometimes called <italic>Ciona robusta</italic>) contains a series of sensory cell types distributed across the head–tail axis of swimming tadpoles. They arise from lateral regions of the neural plate that exhibit properties of vertebrate placodes and neural crest. The sensory determinant <italic>POU IV/Brn3</italic> is known to work in concert with regional determinants, such as <italic>Foxg</italic> and <italic>Neurogenin</italic>, to produce palp sensory cells (PSCs) and bipolar tail neurons (BTNs), in head and tail regions, respectively. A combination of single-cell RNA-sequencing (scRNA-seq) assays, computational analysis, and experimental manipulations suggests that misexpression of <italic>POU IV</italic> results in variable transformations of epidermal cells into hybrid sensory cell types, including those exhibiting properties of both PSCs and BTNs. Hybrid properties are due to coexpression of <italic>Foxg</italic> and <italic>Neurogenin</italic> that is triggered by an unexpected <italic>POU IV</italic> feedback loop. Hybrid cells were also found to express a synthetic gene battery that is not coexpressed in any known cell type. We discuss these results with respect to the opportunities and challenges of reprogramming cell types through the targeted misexpression of cellular determinants.
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A single motor neuron determines the rhythm of early motor behavior in Ciona Reviewed
Taichi Akahoshi, Madoka K. Utsumi, Kouhei Oonuma, Makoto Murakami, Takeo Horie, Takehiro G. Kusakabe, Kotaro Oka, Kohji Hotta
Science Advances 7 ( 50 ) 2021.12
Publisher：American Association for the Advancement of Science (AAAS)
Single motor neuron regulates rhythmic tail flick in prehatching
<italic>Ciona</italic>
embryo. -
Evolution of Developmental Programs for the Midline Structures in Chordates: Insights From Gene Regulation in the Floor Plate and Hypochord Homologues of Ciona Embryos Reviewed International journal
Kouhei Oonuma, Maho Yamamoto, Naho Moritsugu, Nanako Okawa, Megumi Mukai, Miku Sotani, Shuto Tsunemi, Haruka Sugimoto, Eri Nakagome, Yuichi Hasegawa, Kotaro Shimai, Takeo Horie, Takehiro G. Kusakabe
Frontiers in Cell and Developmental Biology 9 ( 704367 ) 704367 - 704367 2021.5
Joint Work
Authorship：Last author, Corresponding author Publisher：Frontiers Media SA
In vertebrate embryos, dorsal midline tissues, including the notochord, the prechordal plate, and the floor plate, play important roles in patterning of the central nervous system, somites, and endodermal tissues by producing extracellular signaling molecules, such as Sonic hedgehog (Shh). In <italic>Ciona</italic>, <italic>hedgehog.b</italic>, one of the two <italic>hedgehog</italic> genes, is expressed in the floor plate of the embryonic neural tube, while none of the <italic>hedgehog</italic> genes are expressed in the notochord. We have identified a <italic>cis</italic>-regulatory region of <italic>hedgehog.b</italic> that was sufficient to drive a reporter gene expression in the floor plate. The <italic>hedgehog.b cis</italic>-regulatory region also drove ectopic expression of the reporter gene in the endodermal strand, suggesting that the floor plate and the endodermal strand share a part of their gene regulatory programs. The endodermal strand occupies the same topographic position of the embryo as does the vertebrate hypochord, which consists of a row of single cells lined up immediately ventral to the notochord. The hypochord shares expression of several genes with the floor plate, including <italic>Shh</italic> and <italic>FoxA</italic>, and play a role in dorsal aorta development. Whole-embryo single-cell transcriptome analysis identified a number of genes specifically expressed in both the floor plate and the endodermal strand in <italic>Ciona</italic> tailbud embryos. A <italic>Ciona</italic> FoxA ortholog FoxA.a is shown to be a candidate transcriptional activator for the midline gene battery. The present findings suggest an ancient evolutionary origin of a common developmental program for the midline structures in Olfactores.
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Kouhei Oonuma, Takehiro G. Kusakabe
Development dev.198754 ( 12 ) dev198754 2021.5
Joint Work
Authorship：Last author Publisher：The Company of Biologists
<title>ABSTRACT</title>
The Ciona larva has served as a unique model for understanding the development of dopaminergic cells at single-cell resolution owing to the exceptionally small number of neurons in its brain and its fixed cell lineage during embryogenesis. A recent study suggested that the transcription factors Fer2 and Meis directly regulate the dopamine synthesis genes in Ciona, but the dopaminergic cell lineage and the gene regulatory networks that control the development of dopaminergic cells have not been fully elucidated. Here, we reveal that the dopaminergic cells in Ciona are derived from a bilateral pair of cells called a9.37 cells at the center of the neural plate. The a9.37 cells divide along the anterior-posterior axis, and all of the descendants of the posterior daughter cells differentiate into the dopaminergic cells. We show that the MAPK pathway and the transcription factor Otx are required for the expression of Fer2 in the dopaminergic cell lineage. Our findings establish the cellular and molecular framework for fully understanding the commitment to dopaminergic cells in the simple chordate brain.DOI： 10.1242/dev.198754
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Cellular identity and Ca2+ signaling activity of the non-reproductive GnRH system in the Ciona intestinalis type A (Ciona robusta) larva. Reviewed International journal
Nanako Okawa, Kotaro Shimai, Kohei Ohnishi, Masamichi Ohkura, Junichi Nakai, Takeo Horie, Atsushi Kuhara, Takehiro G Kusakabe
Scientific reports 10 ( 1 ) 18590 - 18590 2020.10
Joint Work
Authorship：Last author, Corresponding author
Tunicate larvae have a non-reproductive gonadotropin-releasing hormone (GnRH) system with multiple ligands and receptor heterodimerization enabling complex regulation. In Ciona intestinalis type A larvae, one of the gnrh genes, gnrh2, is conspicuously expressed in the motor ganglion and nerve cord, which are homologous structures to the hindbrain and spinal cord, respectively, of vertebrates. The gnrh2 gene is also expressed in the proto-placodal sensory neurons, which are the proposed homologue of vertebrate olfactory neurons. Tunicate larvae occupy a non-reproductive dispersal stage, yet the role of their GnRH system remains elusive. In this study, we investigated neuronal types of gnrh2-expressing cells in Ciona larvae and visualized the activity of these cells by fluorescence imaging using a calcium sensor protein. Some cholinergic neurons and dopaminergic cells express gnrh2, suggesting that GnRH plays a role in controlling swimming behavior. However, none of the gnrh2-expressing cells overlap with glycinergic or GABAergic neurons. A role in motor control is also suggested by a relationship between the activity of gnrh2-expressing cells and tail movements. Interestingly, gnrh2-positive ependymal cells in the nerve cord, known as a kind of glia cells, actively produced Ca2+ transients, suggesting that active intercellular signaling occurs in the glia cells of the nerve cord.
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Spatio-temporal regulation of Rx and mitotic patterns shape the eye-cup of the photoreceptor cells in Ciona Reviewed
Oonuma, K., Kusakabe, T. G.
Developmental Biology 445 ( 2 ) 245 - 255 2019.1
Joint Work
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Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics Reviewed
Horie, T., Horie, R., Chen, K., Cao, C., Nakagawa, M., Kusakabe, T. G., Satoh, N., Sasakura, Y., Levine, M.
Genes & Development 32 ( 19-20 ) 1297 - 1302 2018.10
Joint Work
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The Use of cis-Regulatory DNAs as Molecular Tools
Kotaro Shimai and Takehiro Kusakabe
Advances in Experimental Medicine and Biology 1029 49 - 68 2018.3
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ANISEED 2017: extending the integrated ascidian database to the exploration and evolutionary comparison of genome-scale datasets Reviewed
Brozovic, M., Dantec, C., Dardaillon, J., Dauga, D., Faure, E., Gineste, M., Louis, A., Naville, M., Nitta, K.R., Piette, J., Reeves, W., Scornavacca, C., Simion, P., Vincentelli, R., Bellec, M., Aicha, S.B., Fagotto, M., Guéroult-Bellone, M., Haeussler, M., Jacox, E., Lowe, E.K., Mendez, M., Roberge, A., Stolfi, A., Yokomori, R., Brown, C.T., Cambillau, C., Christiaen, L., Delsuc, F., Douzery, E., Dumollard, R,, Kusakabe, T., Nakai, K., Nishida, H., Satou, Y., Swalla, B., Veeman, M., Volff, J.N., Lemaire, P.
Nucleic Acids Research 46 ( D1 ) D718 - D725 2018.1
Joint Work
DOI： 10.1093/nar/gkx1108
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Constrained vertebrate evolution by pleiotropic genes Reviewed
Hu, H., Uesaka, M., Guo, S., Shimai, K., Lu, T.-M. Li, F., Fujimoto, S., Ishikawa, M., Liu, S., Sasagawa, Y., Zhang, G., Kuratani, S., Yu, J.-K. Kusakabe, T. G., Khaitovich, P., Irie, N., the EXPANDE Consortium
Nature Ecology and Evolution 1 (11), 1722-1730 1 ( 11 ) 1722 - 1730 2017.11
Joint Work
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Evolutionary steps involving counterion displacement in a tunicate opsin Reviewed
Kojima, K., Yamashita, T., Imamoto, Y., Kusakabe, T. G., Tsuda, M., Shichida, Y.
Proceedings of the National Academy of Sciences of the United States of America 114 ( 23 ) 6028 - 6033 2017.6
Joint Work
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Identifying vertebrate brain prototypes in deuterostomes
Takehiro G. Kusakabe
Brain Evolution by Design: From Neural Origin to Cognitive Architecture 153 - 186 2017.2
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Revised lineage of larval photoreceptor cells in Ciona reveals archetypal collaboration between neural tube and neural crest in sensory organ formation Reviewed
Oonuma, K., Tanaka, M., Nishitsuji, K., Kato, Y., Shimai, K., Kusakabe, T. G.
Developmental Biology 420 ( 1 ) 178 - 185 2016.12
Joint Work
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Genome-wide identification and characterization of transcription start sites and promoters in the tunicate Ciona intestinalis Reviewed
Yokomori, R., Shimai, K., Nishitsuji, K., Suzuki, Y., Kusakabe, T. G. and Nakai K.
Genome Research 26 ( 1 ) 140 - 150 2016.1
Joint Work
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The pre-vertebrate origins of neurogenic placodes Reviewed
Abitua, P. A., Gainous, T. B., Kaczmarczyk, A. N., Winchell, C. J., Hudson, C., Kamata, K., Nakagawa, M., Tsuda, M., Kusakabe, T. G. and Levine, M.
Nature 524 ( 7566 ) 462 - 465 2015.8
Joint Work
DOI： 10.1038/nature14657
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Generation of Arabidopsis transformants expressing sphingoid long-chain base kinase (LCBK1)
Daiki Yanagawa, Osamu Tanaka, Takehiro Kusakabe, Daiske Honda, Ayako Gotoh, and Hiroyuki Imai
Memoirs of Konan University, Science and Engineering Series 62 ( 1 ) 1 - 18 2015
Joint Work
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Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis Reviewed
Kamiya, C., Ohta, N., Ogura, Y., Yoshida, K., Horie, T., Kusakabe, T. G., Satake, H., and Sasakura, Y.
Developmental Dynamics 243 ( 12 ) 1524 - 1535 2014.12
Joint Work
DOI： 10.1002/dvdy.24176
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Transcriptional co-regulation of evolutionarily conserved microRNA/cone opsin gene pairs: Implications for photoreceptor subtype specification Reviewed
Daido, Y., Hamanishi, S., and Kusakabe, T. G.
Developmental Biology 392 ( 1 ) 117 - 129 2014.8
Joint Work
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Characterization of the compact bicistronic microRNA precursor, miR-1/miR-133, expressed specifically in Ciona muscle tissues Reviewed
Kusakabe, R., Tani, S., Nishitsuji, K., Shindo, M., Okamura, K., Miyamoto, Y., Nakai, K., Suzuki, Y., Kusakabe, T. G., and Inoue, K.
Gene Expression Patterns 13 ( 1-2 ) 43 - 53 2013.2
Joint Work
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Genome Structure, Functional Genomics, and Proteomics in Ascidians
Yasunori Sasakura, Nicolas Sierro, Kenta Nakai, Kazuo Inaba, and Takehiro G. Kusakabe
Genome Mapping and Genomics in Laboratory Animals. Genome Mapping and Genomics in Animals 4 87 - 132 2013.1
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Transcriptional regulatory regions that promote innexin2 expression in somatic support cells in the Drosophila ovary
Kimura, T., Kita, H., Hira, S., Kuhara, A., Kusakabe, T. G., Michinomae, M., Kobayashi, S., Mukai, M.
Memoirs of Konan University, Science and Engineering Series 60 ( 1 ) 1 - 9 2013
Joint Work
DOI： 10.14990/00000186
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Determination of D-serine in several model organisms used for metabolic, developmental and/or genetic researches by liquid chromatography/fluorescence detection and tandem mass spectrometry
Syuto, M., Kusakabe, T., Honda, D., Watanabe, Y., Takeda, K., Tanaka, O., Imai, H.
Memoirs of Konan University, Science and Engineering Series 60 ( 1 ) 11 - 19 2013
Joint Work
DOI： 10.14990/00000187
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Cis-acting transcriptional repression establishes a sharp boundary in chordate embryos Reviewed
Imai, K. S., Daodo, Y., Kusakabe, T. G., and Satou, Y.
Science 337 ( 6097 ) 964 - 967 2012.8
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A conserved non-reproductive GnRH system in chordates Reviewed
Kusakabe, T. G., Sakai, T., Aoyama, M., Kitajima, Y., Miyamoto, Y., Takigawa, T., Daido, Y., Fujiwara, K., Terashima, Y., Sugiuchi, Y., Matassi, G., Yagisawa, H., Park, M. K., Satake, H., and Tsuda, M.
PLoS One 7 ( 7 ) e41955 - e41955 (12 pages) 2012.7
Joint Work
DOI： 10.1371/journal.pone.0041955
Other Link： http://dx.plos.org/10.1371/journal.pone.0041955
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Monoaminergic modulation of photoreception in ascidian: Evidence for a proto-hypothalamo-retinal territory Reviewed
Razy-Krajka, F., Brown, E. R., Horie, T., Callebert, J., Sasakura, Y., Joly, J. S. Kusakabe, T. G., and Vernier, P.
BMC Biology 10 ( 45 ) 1 - 14 2012.5
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Evidence for differential regulation of GnRH signaling via heterodimerization among GnRH receptor paralogs in the protochordate, Ciona intestinalis Reviewed
Sakai, T., Aoyama, M., Kawada, T., Kusakabe, T., Tsuda, M., and Satake, H.
Endocrinology 153 ( 4 ) 1841 - 1849E 2012.4
Joint Work
DOI： 10.1210/en.2011-1668
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Cell lineage and cis-regulation for a unique GABAergic/glycinergic neuron type in the larval nerve cord of the ascidian Ciona intestinalis Reviewed
Nishitsuji, K., Horie, T., Ichinose, A., Sasakura, Y., Yasuo, H., and Kusakabe, T. G.
Development, Growth and Differentiation 54 ( 2 ) 177 - 186 2012.2
Joint Work
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Profiling ascidian promoters as the primordial type of vertebrate promoter Reviewed
Okamura, K., Yamashita, R., Takimoto, N., Nishitsuji, K., Suzuki, Y., Kusakabe, T. G., and Nakai, K.
BMC Genomics 12 ( Suppl 3 ) S7 - S7 (14 pages) 2011.11
Joint Work
DOI： 10.1186/1471-2164-12-S3-S7
Other Link： http://www.biomedcentral.com/1471-2164/12/S3/S7
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COQ6 mutations in human patients produce nephrotic syndrome with sensorineural deafness Reviewed
Heeringa, S. F., Chernin, G., Chaki, M., Zhou, W., Sloan, A. J., Ji, Z., Xie, L. X., Salviati, L., Hurd, T. W., Vega-Warner, V., Killen, P. D., Raphael, Y., Ashraf, S., Ovunc, B., Schoeb, D. S., McLaughlin, H. M., Airik, R., Vlangos, C. N., Gbadegesin, R., Hinkes, B., Saisawat, P., Trevisson, E., Doimo, M., Casarin, A., Pertegato, V., Giorgi, G., Prokisch, H., Rötig, A., Nürnberg, G., Becker, C., Wang, S., Ozaltin, F., Topaloglu, R., Bakkaloglu, A., Bakkaloglu, S. A., Müller, D., Beissert, A., Mir, S., Berdeli, A., Varpizen, S., Zenker, M., Matejas, V., Santos-Ocaña, C., Navas, P., Kusakabe, T., Kispert, A., Akman, S., Soliman, N. A., Krick, S., Mundel, P., Reiser, J., Nürnberg, P., Clarke, C. F., Wiggins, R. C., Faul, C., and Hildebrandt, F.
Journal of Clinical Investigation 121 ( 5 ) 2013 - 2024 2011.5
Joint Work
DOI： 10.1172/JCI45693
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Cross-validated methods for promoter/transcription start site mapping in SL trans-spliced genes, established using the Ciona intestinalis troponin I gene Reviewed
Khare, P., Mortimer, S., Cleto, C., Okamura, K., Suzuki, Y., Kusakabe, T., Nakai, K., Meedel, T., and Hastings, K.
Nucleic Acids Research 39 ( 7 ) 2638 - 2648 2011.4
Joint Work
DOI： 10.1093/nar/gkq1151
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Ependymal cells of chordate larvae are stem-like cells that form the adult nervous system Reviewed
Horie, T., Shinki, R., Ogura, Y., Kusakabe, T. G., Satoh, N., and Sasakura, Y.
Nature 469 ( 7331 ) 525 - 528 2011.1
Joint Work
DOI： 10.1038/nature09631
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The ANISEED database: Digital representation, formalization, and elucidation of a chordate developmental program Reviewed
Tassy, O., Dauga, D., Daian, F., Sobral, D., Robin, F., Khoueiry, P., Salgado, D., Fox, V., Caillol, D., Schiappa, R., Laporte, B., Rios, A., Luxardi, G., Kusakabe, T., Joly, J.-S., Darras, S., Christiaen, L., Contensin, M., Auger, H., Lamy, C., Hudson, C., Rothbächer, U., Gilchrist, M., Makabe, K. W., Hotta, K., Fujiwara, S., Satoh, N., Satou, Y., and Lemaire, P.
Genome Research 20 ( 10 ) 1459 - 1468 2010.10
Joint Work
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Functional diversity of signaling pathways through G protein-coupled receptor heterodimerization with a species-specific orphan receptor subtype Reviewed
Sakai, T., Aoyama, M., Kusakabe, T., Tsuda, M., and Satake, H.
Molecular Biology and Evolution 27 ( 5 ) 1097 - 1196 2010.5
Joint Work
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Individuals with mutations in XPNPEP3, which encodes a mitochondrial protein, develop a nephronophthisis-like nephropathy Reviewed
O'Toole, J. F., Liu, Y., Davis, E. E., Westlake, C. J., Attanasio, M., Otto, E. A., Seelow, D., Nurnberg, G., Becker, C., Nuutinen, M., Kärppä, M., Ignatius, J., Uusimaa, J., Pakanen, S., Jaakkola, E., van den Heuvel, L. P., Fehrenbach, H., Wiggins, R., Goyal, M., Zhou, W., Wolf, M. T., Wise, E., Helou, J., Allen, S. J., Murga-Zamalloa, C. A., Ashraf, S., Chaki, M., Heeringa, S., Chernin, G., Hoskins, B. E., Chaib, H., Gleeson, J., Kusakabe, T., Suzuki, T., Isaac, R. E., Quarmby, L. M., Tennant, B., Fujioka, H., Tuominen, H., Hassinen, I., Lohi, H., van Houten, J. L., Rotig, A., Sayer, J. A., Rolinski, B., Freisinger, P., Madhavan, S. M., Herzer, M., Madignier, F., Prokisch, H., Nurnberg, P., Jackson, P. K., Khanna, H., Katsanis, N., and Hildebrandt, F.
Journal of Clinical Investigation 120 ( 3 ) 791 - 802 2010.3
Joint Work
DOI： 10.1172/JCI40076
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Distinctive expression patterns of Hedgehog pathway genes in the Ciona intestinalis Reviewed
Islam, A. F. M. T., Moly, P. K., Miyamoto, Y., and Kusakabe, T. G.
Zoological Science 27 ( 2 ) 181 - 190 2010.2
Joint Work
DOI： 10.2108/zsj.27.84
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Simple motor system of the ascidian larva: neuronal complex comprising putative cholinergic neurons and GABAergic/glycinergic neurons Reviewed
Horie, T., Nakagawa, M., Sasakura, Y., Kusakabe, T. G., and Tsuda, M.
Zoological Science 27 ( 2 ) 84 - 90 2010.2
Joint Work
DOI： 10.2108/zsj.27.181
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Evolution and the origin of the visual retinoid cycle in vertebrates Reviewed
Kusakabe, T. G., Takimoto, N., Jin, M., and Tsuda, M.
Philosophical Transactions of the Royal Society B 364 ( 1531 ) 2897 - 2910 2009.10
Joint Work
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Tube formation by complex cellular processes in Ciona intestinalis notochord Reviewed
Dong, B., Horie, T., Denker, E., Kusakabe, T., Tsuda, M., Smith, W. C., and Jiang, D.
Developmental Biology 330 ( 2 ) 237 - 249 2009.6
Joint Work
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Cell type and function of neurons in the ascidian nervous system Reviewed
Horie, T., Sasakura, Y., Nakagawa, M., and Kusakabe, T. G.
Development, Growth & Differentiation 51 ( 3 ) 207 - 220 2009.4
Joint Work
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Pigmented and nonpigmented ocelli in the brain vesicle of the ascidian larva Reviewed
Horie, T., Sakurai, D., Ohtsuki, H., Terakita, A., Shichida, Y., Usukura, J., Kusakabe, T., and Tsuda, M.
Journal of Comparative Neurology 509 ( 1 ) 88 - 102 2008.7
Joint Work
DOI： 10.1002/cne.21733
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Functions of a GnRH receptor heterodimer of the ascidian, Ciona intestinalis Reviewed
Sakai, T., Aoyama, M., Kusakabe, T., Tsuda, M., and Satake, H.
Acta Biologica Hungarica 59 ( Suppl. ) 241 - 243 2008.6
Joint Work
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Glutamatergic networks in the Ciona intestinalis larva Reviewed
Horie, T., Kusakabe, T., and Tsuda, M.
Journal of Comparative Neurology 508 ( 2 ) 249 - 263 2008.5
Joint Work
DOI： 10.1002/cne.21678
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Markov chain-based promoter structure modeling for tissue-specific expression pattern prediction Reviewed
Vandenbon, A., Miyamoto, Y., Takimoto, N., Kusakabe, T. and Nakai, K.
DNA Research 15 ( 1 ) 3 - 11 2008.2
Joint Work
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Comparative genomics identifies a cis-regulatory module that activates transcription in specific subsets of neurons in Ciona intestinalis larvae Reviewed
Yoshida, R., Horie, T., Tsuda, M. and Kusakabe, T. G.
Development, Growth and Differentiation 48 ( 8 ) 657 - 667 2007.10
Joint Work
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Origin of the vertebrate visual cycle Reviewed
Takimoto, N., Kusakabe, T., and Tsuda, M.
Photochemistry and Photobiology 83 ( 2 ) 242 - 247 2007.3
Joint Work
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Photoreceptive systems in ascidians Reviewed
Kusakabe, T. and Tsuda, M.
Photochemistry and Photobiology 83 ( 2 ) 248 - 252 2007.3
Joint Work
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Origin of the vertebrate visual cycle: III. Distinct distribution of RPE65 and β-carotene 15,15'-monooxygenase homologues in Ciona intestinalis Reviewed
Takimoto, N., Kusakabe, T., Horie, T., Miyamoto, Y., and Tsuda, M.
Photochemistry and Photobiology 82 ( 6 ) 1468 - 1474 2006.11
Joint Work
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The centrosomal protein nephrocystin-6 is mutated in Joubert syndrome and activates transcription factor ATF4 Reviewed
Sayer, J.A., Otto, E.A., O'toole, J.F., Nurnberg, G., Kennedy, M.A., Becker, C., Hennies, H.C., Helou, J., Attanasio, M., Fausett, B.V., Utsch, B., Khanna, H., Liu, Y., Drummond, I., Kawakami, I., Kusakabe, T., Tsuda, M., Ma, L., Lee, H., Larson, R.G., Allen, S.J., Wilkinson, C.J., Nigg, E.A., Shou, C., Lillo, C., Williams, D.S., Hoppe, B., Kemper, M.J., Neuhaus, T., Parisi, M.A., Glass, I.A., Petry, M., Kispert, A., Gloy, J., Ganner, A., Walz, G., Zhu, X., Goldman, D., Nurnberg, P., Swaroop, A., Leroux, M.R., and Hildebrandt, F.
Nature Genetics 38 ( 6 ) 674 - 681 2006.6
Joint Work
DOI： 10.1038/ng1786
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DBTGR: a database of tunicate promoters and their regulatory elements Reviewed
Sierro, N., Kusakabe, T., Park, K.-J., Yamashita, R., Kinoshita, K., and Nakai, K.
Nucleic Acids Research 34 D552 - D555 2006.1
Joint Work
DOI： 10.1093/nar/gkj064
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Evolution of constrained GnRH ligand conformation and receptor sensitivity Reviewed
Barran, P.E., Roeske, R.W., Pawson, A.J., Sellar, R., Bowers, M.T., Morgan, K., Lu, Z.-L., Tsuda, M., Kusakabe, T., and Millar, R.P.
Journal of Biological Chemistry 280 ( 46 ) 38568 - 38575 2005.11
Joint Work
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Nephrocystin-5, a ciliary IQ domain protein, is mutated in Senior-Loken syndrome and interacts with RPGR and calmodulin Reviewed
Otto, E.A., Loeys, B., Khanna, H., Hellemans, J., Sudbrak, R., Fan, S., Muerb, U., O'Toole, J.F., Helou, J., Attanasio, M., Utsch, B., Sayer, J.A., Lillo, C., Jimeno, D., Coucke, P., De Paepe, A., Reinhardt, R., Klages, S., Tsuda, M., Kawakami, I., Kusakabe, T., Omran, H., Imm, A., Tippens, M., Raymond, P.A., Hill, J., Beales, P., He, S., Kispert, A., Margolis, B., Williams, D.S., Swaroop, A., and Hildebrandt, F.
Nature Genetics 37 ( 3 ) 282 - 288 2005.3
Joint Work
DOI： 10.1038/ng1520
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Decoding cis-regulatory systems in ascidians Reviewed
Kusakabe, T.
Zoological Science 22 ( 2 ) 129 - 146 2005.2
Single Work
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Computational discovery of DNA motifs associated with cell type-specific gene expression in Ciona Reviewed
Kusakabe, T., Yoshida, R., Ikeda, Y., and Tsuda, M.
Developmental Biology 276 ( 2 ) 129 - 146 2004.12
Joint Work
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Localization of mRNAs encoding alpha and beta subunits of soluble guanylyl cyclase in the brain of rainbow trout: comparison with the distribution of neuronal nitric oxide synthase Reviewed
Ando, H., Shi, Q., Kusakabe, T., Ohya, T., Suzuki, N., and Urano, A.
Brain Research 1013 ( 1 ) 13 - 29 2004.7
Joint Work
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Identification of neuron-specific promoters in Ciona intestinalis Reviewed
Yoshida, R., Sakurai, D., Horie, T., Kawakami, I., Tsuda, M., and Kusakabe, T.
Genesis 39 ( 2 ) 130 - 140 2004.6
Joint Work
DOI： 10.1002/gene.20032
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Muscle actin genes in the ascidian Halocynthia roretzi: structure, organization and transcriptional regulation
Takehiro Kusakabe
2004.3
Single Work
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Structure, expression, and cluster organization of genes encoding gonadotropin-releasing hormone receptors found in the neural complex of the ascidian Ciona intestinalis Reviewed
Kusakabe, T., Mishima, S., Shimada, I., Kitajima, Y., and Tsuda, M.
Gene 322 77 - 84 2003.12
Joint Work
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Origin of the vertebrate visual cycle. II. Visual cycle proteins are localized in whole brain including photoreceptor cells of a primitive chordate Reviewed
Tsuda, M., Kusakabe, T., Iwamoto, H., Horie, T., Nakashima, Y., Nakagawa, M., and Okunou, K.
Vision Research 43 ( 28 ) 3045 - 3053 2003.12
Joint Work
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Targeted knockdown of an opsin gene inhibits the swimming behaviour photoresponse of ascidian larvae Reviewed
Inada, K., Horie, T., Kusakabe, T., and Tsuda, M.
Neuroscience Letters 347 ( 3 ) 167 - 170 2003.8
Joint Work
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Origin of the vertebrate visual cycle: genes encoding retinal photoisomerase and two putative visual cycle proteins are expressed in whole brain of a primitive chordate Reviewed
Nakashima, Y., Kusakabe, T., Kusakabe, R., Terakita, A., Shichida, Y., and Tsuda, M.
Journal of Comparative Neurology 460 ( 2 ) 180 - 190 2003.5
Joint Work
DOI： 10.1002/cne.10645
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Central nervous system-specific expression of G protein α-subunits in the ascidian Ciona intestinalis Reviewed
Yoshida, R., Kusakabe, T., Kamatani, M., Daitoh, M., and Tsuda, M.
Zoological Science 19 ( 10 ) 1079 - 1088 2002.10
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Phototransduction and visual cycle in the ascidian tadpole larva Reviewed
Kusakabe, T., Nakashima, Y., Kusakabe, R., Takeo, H., Kawakami, I., Yoshida, R., Inada, K., Nakagawa, M., and Tsuda, M.
Journal of Photoscience 9 ( 2 ) 37 - 40 2002.8
Joint Work
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Ciona intestinalis cDNA projects: EST analyses and gene expression profiles during embryogenesis Reviewed
Satou, Y., Takatori, N., Fujiwara, S., Nishikata, T., Saiga, H., Kusakabe, T., Shin-i, T., Kohara, Y., and Satoh, N.
Gene 287 ( 1-2 ) 83 - 96 2002.4
Joint Work
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Gene expression profiles in tadpole larvae of Ciona intestinalis
Kusakabe, T., Yoshida, R., Kawakami, I., Kusakabe, R., Mochizuki, Y., Yamada, L., Shin-i, T., Kohara, K., Satoh, N., Tsuda, M., and Satou, Y.
Developmental Biology 242 ( 2 ) 188 - 203 2002.2
Joint Work
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Ci-opsin1, a vertebrate-type opsin gene, expressed in the larval ocellus of the ascidian Ciona intestinalis Reviewed
Kusakabe, T., Kusakabe, R., Kawakami, K., Satou, Y., Satoh, N., and Tsuda, M.
FEBS Letters 506 ( 1 ) 69 - 72 2001.9
Joint Work
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A cis-regulatory element essential for photoreceptor-specific expression of a medaka retinal guanylyl cyclase gene Reviewed
Kusakabe, T. and Suzuki, N.
Development Genes and Evolution 211 ( 3 ) 145 - 149 2001.3
Joint Work
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Photoreceptors and olfactory cells express the same retinal guanylyl cyclase isoform in medaka: visualization by promoter transgenics Reviewed
Kusakabe, T. and Suzuki, N.
FEBS Letters 483 ( 2-3 ) 143 - 148 2000.10
Joint Work
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A detailed linkage map of medaka, Oryzias latipes: comparative genomics and genome evolution Reviewed
Naruse, K., Fukamachi, S., Mitani, H., Kondo, M., Matsuoka, T., Kondo, S., Hamamura, N., Morita, Y., Hasegawa, K., Nishigaki, R., Shimada, A., Wada, H., Kusakabe, T., Suzuki, N., Kinoshita, M., Kanamori, A., Terado, T., Kimura, H., Nonaka, M., and Shima, A.
Genetics 154 ( 4 ) 1773 - 1784 2000.4
Joint Work
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The guanylyl cyclase family in medaka fish Oryzias latipes Reviewed
Kusakabe, T. and Suzuki, N.
Zoological Science 17 ( 2 ) 131 - 140 2000.2
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In vivo analysis of two striated muscle actin promoters reveals combinations of multiple regulatory modules required for skeletal and cardiac muscle-specific gene expression Reviewed
Kusakabe, R., Kusakabe, T., and Suzuki, N.
International Journal of Developmental Biology 43 ( 6 ) 541 - 554 1999.9
Joint Work
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Tandem organization of medaka fish soluble guanylyl cyclase α1 and β1 subunit genes: implications for coordinated transcription of two subunit genes Reviewed
Mikami, T., Kusakabe, T., and Suzuki, N.
Journal of Biological Chemistry 274 ( 26 ) 18567 - 18573 1999.6
Joint Work
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Evolution of the ascidian anural larva: evidence from embryos and molecules Reviewed
Jeffery, W. R., Billie J. Swalla, B. J., Ewing, N., and Kusakabe, T.
Molecular Biology and Evolution 16 ( 5 ) 646 - 654 1999.5
Joint Work
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Sequence analysis of cDNA and genomic DNA, and mRNA expression of the medaka fish homolog of the mammalian guanylyl cyclase C Reviewed
Mantoku, T., Muramatsu, R., Nakauchi, M., Yamagami, S., Kusakabe, T., and Suzuki, N.
Journal of Biochemistry 125 ( 3 ) 476 - 486 1999.3
Joint Work
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Genomic organization and evolution of actin genes in the amphioxus Branchiostoma belcheri and Branchiostoma floridae Reviewed
Kusakabe, R., Satoh, N., Holland, L. Z., and Kusakabe, T.
Gene 227 ( 1 ) 1 - 10 1999.2
Joint Work
-
Sequence analysis of cDNAs and genomic DNA, and mRNA expression of the medaka fish homolog of the mammalian guanylyl cyclase C (共著)
日下部 岳広
Journal of Biochemistry 125 ( 3 ) 476 - 486 1999
Single Work
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Molecular cloning of cDNAs and expression of mRNAs encoding a and b subunits of soluble guanylyl cyclase from medaka fish Oryzias latipes Reviewed
Mikami, T., Kusakabe, T., and Suzuki, N.
European Journal of Biochemistry 253 ( 1 ) 42 - 48 1998.4
Joint Work
-
Ascidian actin genes: developmental regulation of gene expression and molecular evolution Reviewed
Kusakabe, T.
Zoological Science 14 ( 5 ) 707 - 718 1997.10
Single Work
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Differential gene expression and intracellular mRNA localization of amphioxus actin isoforms throughout development: implications for conserved mechanisms of chordate development Reviewed
Kusakabe, R., Kusakabe, T., Satoh, N., Holland, N. D., and Holland, L. Z.
Development Genes and Evolution 207 ( 4 ) 203 - 215 1997.10
Joint Work
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Primary structure and differential gene expression of three membrane forms of guanylyl cyclases found in the eye of the teleost Oryzias latipes Reviewed
Seimiya, M., Kusakabe, T., and Suzuki, N.
Journal of Biological Chemistry 272/37, 23407-23417 272 ( 37 ) 23407 - 23417 1997.9
Joint Work
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Tunic cuticular protrusions in ascidians (Chordata, Tunicata): a perspective of their character-state distribution Reviewed
Hirose, E., Lambert, G., Kusakabe, T., and Nishikawa, T.
Zoological Science 14 ( 4 ) 683 - 689 1997.4
Joint Work
DOI： 10.2108/zsj.14.683
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Evolution of chordate actin genes: evidence from genomic organization and amino acid sequences Reviewed
Kusakabe, T., Araki, I., Satoh, N., and Jeffery, W. R.
Journal of Molecular Evolution 44 ( 3 ) 289 - 298 1997.3
Joint Work
DOI： 10.1007/PL00006146
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Predominant expression of a cytoskeletal actin gene in mesenchyme cells during embryogenesis of the ascidian Halocynthia roretzi Reviewed
Araki, I., Tagawa, K., Kusakabe, T., and Satoh, N.
Development, Growth & Differentiation 38 ( 4 ) 401 - 411 1996.8
Joint Work
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Mechanism of an evolutionary change in muscle cell differentiation in ascidians with different modes of development Reviewed
Kusakabe, T., Swalla, B. J., Satoh, N., and Jeffery, W. R.
Developmental Biology 174 ( 2 ) 379 - 392 1996.3
Joint Work
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Timing of initiation of muscle-specific gene expression in the ascidian embryo precedes that of developmental fate restriction in lineage cells Reviewed
Satou, Y., Kusakabe, T., Araki, I., and Satoh, N.
Development, Growth & Differentiation 37 ( 3 ) 319 - 327 1995.6
Joint Work
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Coexpression and promoter function in two muscle actin gene complexes of different structural organization in the ascidian Halocynthia roretzi Reviewed
Kusakabe, T., Hikosaka, A., and Satoh, N.
Developmental Biology 169 ( 2 ) 461 - 472 1995.6
Joint Work
-
Expression of larval-type muscle actin-encoding genes in the ascidian Halocynthia roretzi Reviewed
Kusakabe, T.
Gene 153 ( 2 ) 215 - 218 1995.2
Single Work
-
Short upstream sequences associated with the muscle-specific expression of an actin gene in ascidian embryos Reviewed
Hikosaka, A., Kusakabe, T., and Satoh, N.
Developmental Biology 166 ( 2 ) 763 - 769 1994.12
Joint Work
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Introduction and expression of recombinant genes in ascidian embryos Reviewed
Hikosaka, A., Kusakabe, T., Satoh, N., and Makabe, K. W.
Development, Growth & Differentiation 34 ( 6 ) 627 - 634 1992.12
Joint Work
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Tunicate muscle actin genes: structure and organization as a gene cluster Reviewed
Kusakabe, T., Makabe, K. W., and Satoh, N.
Journal of Molecular Biology 227 ( 3 ) 955 - 960 1992.10
Joint Work
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Temporal and spatial expression of a muscle actin gene during embryogenesis of the ascidian Halocynthia roretzi Reviewed
Kusakabe, T., Suzuki, J., Saiga, H., Jeffery, W. R., Makabe, K. W., and Satoh, N.
Development, Growth & Differentiation 33 ( 3 ) 227 - 234 1991.6
Joint Work